DOHH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOHH
Entrez Gene ID	83475
Full Name	deoxyhypusine hydroxylase
Synonyms	HLRC1,hDOHH
General protein information	Preferred Names deoxyhypusine hydroxylase Names deoxyhypusine hydroxylase HEAT-like (PBS lyase) repeat containing 1 HEAT-like repeat-containing protein 1 deoxyhypusine dioxygenase deoxyhypusine hydroxylase/monooxygenase deoxyhypusine monooxygenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009].
Disorder MIM:	611262

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528340.1	XP_011526642.1	deoxyhypusine hydroxylase isoform X1
NM_031304.5	NP_112594.1	deoxyhypusine hydroxylase
XM_011528341.2	XP_011526643.1	deoxyhypusine hydroxylase isoform X1
NM_001145165.1	NP_001138637.1	deoxyhypusine hydroxylase
NM_031304.4	NP_112594.1	deoxyhypusine hydroxylase
NM_001145165.2	NP_001138637.1	deoxyhypusine hydroxylase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOHH	NP_001069354.1
Drosophila melanogaster (fruit fly)	nero	NP_651887.1
Magnaporthe oryzae (rice blast fungus)	MGG_01725	XP_003714722.1
Oryza sativa (rice)	Os12g0163300	NP_001066229.1
Homo sapiens (human)	DOHH	NP_112594.1
Pan troglodytes (chimpanzee)	DOHH	XP_512268.3
Mus musculus (house mouse)	Dohh	NP_598725.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002129	XP_320906.3
Caenorhabditis elegans (roundworm)	dohh-1	NP_496279.1
Kluyveromyces lactis	KLLA0B14080g	XP_452158.1
Arabidopsis thaliana (thale cress)	AT3G58180	NP_567062.1
	DOHH	XP_001100385.1
Canis lupus familiaris (dog)	LOC485060	XP_542178.2
Rattus norvegicus (Norway rat)	Dohh	NP_001020177.1
Saccharomyces cerevisiae (baker's yeast)	LIA1	NP_012604.1
Oryza sativa (rice)	Os12g0626100	NP_001067322.2
Gallus gallus (chicken)	DOHH	NP_001026584.1
Danio rerio (zebrafish)	dohh	NP_955857.1
Eremothecium gossypii	AGOS_AFR426C	NP_985973.2
Schizosaccharomyces pombe (fission yeast)	mmd1	NP_594654.1
Neurospora crassa	NCU05252	XP_961789.1
Xenopus tropicalis (tropical clawed frog)	LOC100145112	NP_001120098.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Han Z, Sakai N, B?ttger LH, Klinke S, Hauber J, Trautwein AX, Hilgenfeld R
Structure (London, England : 1993)23(5)882-892(2015 May)

Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase.
Frey AG, Nandal A, Park JH, Smith PM, Yabe T, Ryu MS, Ghosh MC, Lee J, Rouault TA, Park MH, Philpott CC
Proceedings of the National Academy of Sciences of the United States of America111(22)8031-6(2014 Jun)

Regulation of expression of deoxyhypusine hydroxylase (DOHH), the enzyme that catalyzes the activation of eIF5A, by miR-331-3p and miR-642-5p in prostate cancer cells.
Epis MR, Giles KM, Kalinowski FC, Barker A, Cohen RJ, Leedman PJ
The Journal of biological chemistry287(42)35251-9(2012 Oct)

Expression of eukaryotic initiation factor 5A and hypusine forming enzymes in glioblastoma patient samples: implications for new targeted therapies.
Preukschas M, Hagel C, Schulte A, Weber K, Lamszus K, Sievert H, P?llmann N, Bokemeyer C, Hauber J, Braig M, Balabanov S
PloS one7(8)e43468(2012)

Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center.
Vu VV, Emerson JP, Martinho M, Kim YS, M?nck E, Park MH, Que L Jr
Proceedings of the National Academy of Sciences of the United States of America106(35)14814-9(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data describes 3-D structure of hDOHH in two states, hDOHHperoxo (POX) and a complex of the diiron core with glycerol (GLC) providing an explanation for the extreme longevity of POX and illustrate its substrate specificity for deoxyhypusine-eIF-5A.
Title: Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.

Here, we show that PCBP1 and PCBP2 also deliver iron to deoxyhypusine hydroxylase (DOHH), the dinuclear iron enzyme required for hypusine modification of the translation factor eukaryotic initiation factor 5A.
Title: Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase.

a novel role for miR-331-3p and miR-642-5p in the control of prostate cancer cell growth via the regulation of DOHH expression and eIF5A activity.
Title: Regulation of expression of deoxyhypusine hydroxylase (DOHH), the enzyme that catalyzes the activation of eIF5A, by miR-331-3p and miR-642-5p in prostate cancer cells.

eIF-5A as well as the hypusine-forming enzymes deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) are highly overexpressed in glioblastoma patient sample
Title: Expression of eukaryotic initiation factor 5A and hypusine forming enzymes in glioblastoma patient samples: implications for new targeted therapies.

Resonance Raman experiments show that its blue chromophore arises from a (mu-1,2-peroxo)diiron(III) center that forms in the reaction of the reduced enzyme with O2, so the peroxo form of hDOHH is unusually stable.
Title: Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center.

The following DOHH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOHH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02459	NM_001145165.2 Latest version!	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu02459	NM_031304.5 Latest version!	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu02459	XM_011528340.1 Latest version!	Homo sapiens deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu73069	XM_011528341.2 Latest version!	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu02459	NM_031304.4	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu02459	NM_001145165.1	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOHH ( NM_031304.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_112594.1 Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02459
Clone ID Related Accession (Same CDS sequence)	XM_011528340.1 , NM_031304.5 , NM_001145165.1 , NM_031304.4 , NM_001145165.2
Accession Version	NM_031304.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	deoxyhypusine hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005787.1, BC002817.2 and BU624567.1. On Jun 2, 2019 this sequence version replaced NM_031304.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC002817.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112594.1
CDS	215..1123
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

Target ORF information:

RefSeq Version	NM_031304.5
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031304.5

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOHH ( NM_001145165.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138637.1 Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02459
Clone ID Related Accession (Same CDS sequence)	XM_011528340.1 , NM_031304.5 , NM_001145165.1 , NM_031304.4 , NM_001145165.2
Accession Version	NM_001145165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	deoxyhypusine hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW195139.1, BG491509.1, BC002817.2, BU624567.1 and BC009863.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.301907.1, SRR1163657.572991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138637.1
CDS	453..1361
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

Target ORF information:

RefSeq Version	NM_001145165.1
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145165.1

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOHH ( NM_031304.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_112594.1 Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02459
Clone ID Related Accession (Same CDS sequence)	XM_011528340.1 , NM_031304.5 , NM_001145165.1 , NM_031304.4 , NM_001145165.2
Accession Version	NM_031304.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	deoxyhypusine hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002817.2, BU624567.1 and BC009863.2. On Feb 14, 2009 this sequence version replaced NM_031304.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC002817.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112594.1
CDS	164..1072
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

Target ORF information:

RefSeq Version	NM_031304.4
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031304.4

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOHH ( NM_001145165.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138637.1 Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.

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CloneID	OHu02459
Clone ID Related Accession (Same CDS sequence)	XM_011528340.1 , NM_031304.5 , NM_001145165.1 , NM_031304.4 , NM_001145165.2
Accession Version	NM_001145165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	deoxyhypusine hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG491509.1, BC002817.2 and BU624567.1. On Jul 5, 2019 this sequence version replaced NM_001145165.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.967830.1, SRR3476690.908221.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138637.1
CDS	187..1095
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

Target ORF information:

RefSeq Version	NM_001145165.2
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145165.2

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOHH ( XM_011528340.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526642.1 Homo sapiens deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu02459
Clone ID Related Accession (Same CDS sequence)	XM_011528340.1 , NM_031304.5 , NM_001145165.1 , NM_031304.4 , NM_001145165.2
Accession Version	XM_011528340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	deoxyhypusine hydroxylase isoform X1
Comment

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526642.1
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

Target ORF information:

RefSeq Version	XM_011528340.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens deoxyhypusine hydroxylase/monooxygenase (DOHH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528340.1

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGTGGCCGAG 
ACCTGCCAGC TGGCCGTGCG CAGGCTGGAG TGGCTGCAGC AGCACGGCGG GGAGCCGGCG 
GCGGGACCCT ACCTCTCCGT GGACCCTGCC CCGCCGGCTG AGGAGCGTGA CGTGGGGCGC 
CTGCGGGAGG CGCTGCTGGA TGAGTCCCGG CCGCTCTTCG AGCGATACCG CGCCATGTTC 
GCCCTGCGCA ACGCGGGAGG CGAGGAGGCC GCCCTGGCGC TGGCCGAGGG TCTGCACTGT 
GGGAGCGCCC TCTTCCGCCA CGAGGTCGGC TACGTCCTGG GACAGCTGCA GCACGAGGCG 
GCGGTGCCCC AGCTGGCGGC CGCCCTGGCC CGATGCACCG AGAACCCCAT GGTGCGGCAC 
GAGTGCGCGG AGGCCCTGGG CGCCATTGCC CGGCCCGCCT GCCTGGCCGC GCTGCAGGCT 
CACGCGGACG ACCCAGAGCG CGTGGTGCGT GAGAGCTGCG AGGTGGCTCT GGACATGTAT 
GAGCACGAGA CCGGGCGGGC CTTCCAGTAC GCGGACGGCC TGGAGCAGCT GCGCGGGGCC 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOHH ( XM_011528341.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526643.1 Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73069
Clone ID Related Accession (Same CDS sequence)	XM_011528341.2
Accession Version	XM_011528341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	deoxyhypusine hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGGCGACCAC 
TTGGCCAAGG ATGCCCCAGG GATGAGGAAG GCGAGGGCGA CAATCTGGAC AGACACTTGC 
TGCGCCCCAT TTGGCAAAAC GGCGACAACC AGCTTTGCTC ACCCGCTTCC ATCCCGGGCC 
TTGAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526643.1
CDS	161..652
Translation	MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEGDHLAKDAPGMRKARATIWTDTCCAPFGKTATTSFAHPLPSRALSP

Target ORF information:

RefSeq Version	XM_011528341.2
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxyhypusine hydroxylase (DOHH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528341.2

ORF Insert Sequence:

ATGGTGACGG AGCAGGAGGT GGATGCCATC GGGCAGACGC TGGTGGACCC CAAGCAGCCC 
CTGCAGGCCC GCTTCCGGGC GCTGTTCACG CTGCGTGGGC TCGGCGGCCC AGGCGCCATT 
GCATGGATCA GCCAGGCCTT CGATGACGAT TCCGCCCTGC TCAAGCACGA GCTGGCCTAC 
TGCCTGGGCC AGATGCAGGA TGCCCGCGCC ATCCCCATGC TGGTGGACGT GCTGCAAGAC 
ACCCGTCAGG AGCCCATGGT GCGCCATGAG GCAGGGGAGG CCCTGGGGGC CATCGGGGAC 
CCGGAAGTTC TGGAGATCCT GAAGCAGTAT TCCTCGGACC CCGTCATCGA GGGCGACCAC 
TTGGCCAAGG ATGCCCCAGG GATGAGGAAG GCGAGGGCGA CAATCTGGAC AGACACTTGC 
TGCGCCCCAT TTGGCAAAAC GGCGACAACC AGCTTTGCTC ACCCGCTTCC ATCCCGGGCC 
TTGAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Structure (London, England : 1993)23(5)882-892(2015 May)
Han Z,Sakai N,B?ttger LH,Klinke S,Hauber J,Trautwein AX,Hilgenfeld R

Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase.
Proceedings of the National Academy of Sciences of the United States of America111(22)8031-6(2014 Jun)
Frey AG,Nandal A,Park JH,Smith PM,Yabe T,Ryu MS,Ghosh MC,Lee J,Rouault TA,Park MH,Philpott CC

Regulation of expression of deoxyhypusine hydroxylase (DOHH), the enzyme that catalyzes the activation of eIF5A, by miR-331-3p and miR-642-5p in prostate cancer cells.
The Journal of biological chemistry287(42)35251-9(2012 Oct)
Epis MR,Giles KM,Kalinowski FC,Barker A,Cohen RJ,Leedman PJ

Expression of eukaryotic initiation factor 5A and hypusine forming enzymes in glioblastoma patient samples: implications for new targeted therapies.
PloS one7(8)e43468(2012)
Preukschas M,Hagel C,Schulte A,Weber K,Lamszus K,Sievert H,P?llmann N,Bokemeyer C,Hauber J,Braig M,Balabanov S

Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center.
Proceedings of the National Academy of Sciences of the United States of America106(35)14814-9(2009 Sep)
Vu VV,Emerson JP,Martinho M,Kim YS,M?nck E,Park MH,Que L Jr

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DOHH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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