H2AC18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2AC18
Entrez Gene ID	8337
Full Name	histone cluster 2 H2A family member a3
Synonyms	H2A,H2A.2,H2A/O,H2A/q,H2AFO,H2a-615,HIST2H2AA
General protein information	Preferred Names histone cluster 2 H2A family member a3 Names histone H2A type 2-A H2A histone family, member O histone 2, H2aa3 histone cluster 2, H2aa3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015].
Disorder MIM:	142720

mRNA and Protein(s)

mRNA	Protein	Name
NM_003516.2	NP_003507.1	histone H2A type 2-A
NM_003516.3	NP_003507.1	histone H2A type 2-A

Pathways from BioSystems

Homologies

Homo sapiens (human)	HIST2H2AA4	NP_001035807.1
Mus musculus (house mouse)	Hist2h2aa1	NP_038577.1
Rattus norvegicus (Norway rat)	LOC690131	XP_003749398.1
Rattus norvegicus (Norway rat)	Hist2h2aa3	XP_345256.3
Pan troglodytes (chimpanzee)	HIST2H2AA4	XP_003949577.1
Bos taurus (cattle)	LOC788724	XP_002686133.1
Homo sapiens (human)	HIST2H2AA3	NP_003507.1
	LOC696463	XP_001084365.1
Mus musculus (house mouse)	Hist2h2aa2	NP_835584.1

Gene Ontology
Bibliography

Related articles in PubMed

A subset of replication-dependent histone mRNAs are expressed as polyadenylated RNAs in terminally differentiated tissues.
Lyons SM, Cunningham CH, Welch JD, Groh B, Guo AY, Wei B, Whitfield ML, Xiong Y, Marzluff WF
Nucleic acids research44(19)9190-9205(2016 Nov)

RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK
Cell150(6)1182-95(2012 Sep)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Functional characterization of a human histone gene cluster duplication.
Braastad CD, Hovhannisyan H, van Wijnen AJ, Stein JL, Stein GS
Gene342(1)35-40(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubiquitin-dependent signaling during the DNA damage response (DDR) to double-strand breaks is initiated by two E3 ligases, RNF8 and RNF168, targeting histone H2A and H2AX. Study shows that ubiquitin chains per se are insufficient for signaling, but RNF168 target ubiquitination is required for DDR damage.
Title: RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.

The following H2AC18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2AC18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02148	NM_003516.3 Latest version!	Homo sapiens H2A clustered histone 18 (H2AC18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02148	NM_003516.2	Homo sapiens histone cluster 2 H2A family member a3 (HIST2H2AA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2AC18 ( NM_003516.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003507.1 Homo sapiens histone cluster 2 H2A family member a3 (HIST2H2AA3), mRNA.

Price & Availability↑

CloneID	OHu02148
Clone ID Related Accession (Same CDS sequence)	NM_003516.2 , NM_003516.3
Accession Version	NM_003516.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	histone H2A type 2-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L19779.1. On Jun 7, 2002 this sequence version replaced NM_003516.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: L19779.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGTC GTGGCAAGCA AGGAGGCAAG GCCCGCGCCA AGGCCAAGTC GCGCTCGTCC 
CGCGCTGGCC TTCAGTTCCC GGTAGGGCGA GTGCATCGCT TGCTGCGCAA AGGCAACTAC 
GCGGAGCGAG TGGGGGCCGG CGCGCCCGTC TACATGGCTG CGGTCCTCGA GTATCTGACC 
GCCGAGATCC TGGAGCTGGC GGGCAACGCG GCTCGGGACA ACAAGAAGAC GCGCATCATC 
CCTCGTCACC TCCAGCTGGC CATCCGCAAC GACGAGGAAC TGAACAAGCT GCTGGGCAAA 
GTCACCATCG CCCAGGGCGG CGTCTTGCCT AACATCCAGG CCGTACTGCT CCCTAAGAAG 
ACGGAGAGTC ACCACAAGGC AAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003507.1
CDS	54..446
Translation	MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Target ORF information:

RefSeq Version	NM_003516.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 2 H2A family member a3 (HIST2H2AA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003516.2

ORF Insert Sequence:

ATGTCTGGTC GTGGCAAGCA AGGAGGCAAG GCCCGCGCCA AGGCCAAGTC GCGCTCGTCC 
CGCGCTGGCC TTCAGTTCCC GGTAGGGCGA GTGCATCGCT TGCTGCGCAA AGGCAACTAC 
GCGGAGCGAG TGGGGGCCGG CGCGCCCGTC TACATGGCTG CGGTCCTCGA GTATCTGACC 
GCCGAGATCC TGGAGCTGGC GGGCAACGCG GCTCGGGACA ACAAGAAGAC GCGCATCATC 
CCTCGTCACC TCCAGCTGGC CATCCGCAAC GACGAGGAAC TGAACAAGCT GCTGGGCAAA 
GTCACCATCG CCCAGGGCGG CGTCTTGCCT AACATCCAGG CCGTACTGCT CCCTAAGAAG 
ACGGAGAGTC ACCACAAGGC AAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2AC18 ( NM_003516.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003507.1 Homo sapiens H2A clustered histone 18 (H2AC18), mRNA.

Price & Availability↑

CloneID	OHu02148
Clone ID Related Accession (Same CDS sequence)	NM_003516.2 , NM_003516.3
Accession Version	NM_003516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone H2A type 2-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC239868.2. On Dec 3, 2019 this sequence version replaced NM_003516.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: DA436359.1, CB998207.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGTC GTGGCAAGCA AGGAGGCAAG GCCCGCGCCA AGGCCAAGTC GCGCTCGTCC 
CGCGCTGGCC TTCAGTTCCC GGTAGGGCGA GTGCATCGCT TGCTGCGCAA AGGCAACTAC 
GCGGAGCGAG TGGGGGCCGG CGCGCCCGTC TACATGGCTG CGGTCCTCGA GTATCTGACC 
GCCGAGATCC TGGAGCTGGC GGGCAACGCG GCTCGGGACA ACAAGAAGAC GCGCATCATC 
CCTCGTCACC TCCAGCTGGC CATCCGCAAC GACGAGGAAC TGAACAAGCT GCTGGGCAAA 
GTCACCATCG CCCAGGGCGG CGTCTTGCCT AACATCCAGG CCGTACTGCT CCCTAAGAAG 
ACGGAGAGTC ACCACAAGGC AAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003507.1
CDS	53..445
Translation	MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Target ORF information:

RefSeq Version	NM_003516.3
Organism	Homo sapiens(human)
Definition	Homo sapiens H2A clustered histone 18 (H2AC18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003516.3

ORF Insert Sequence:

ATGTCTGGTC GTGGCAAGCA AGGAGGCAAG GCCCGCGCCA AGGCCAAGTC GCGCTCGTCC 
CGCGCTGGCC TTCAGTTCCC GGTAGGGCGA GTGCATCGCT TGCTGCGCAA AGGCAACTAC 
GCGGAGCGAG TGGGGGCCGG CGCGCCCGTC TACATGGCTG CGGTCCTCGA GTATCTGACC 
GCCGAGATCC TGGAGCTGGC GGGCAACGCG GCTCGGGACA ACAAGAAGAC GCGCATCATC 
CCTCGTCACC TCCAGCTGGC CATCCGCAAC GACGAGGAAC TGAACAAGCT GCTGGGCAAA 
GTCACCATCG CCCAGGGCGG CGTCTTGCCT AACATCCAGG CCGTACTGCT CCCTAAGAAG 
ACGGAGAGTC ACCACAAGGC AAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A subset of replication-dependent histone mRNAs are expressed as polyadenylated RNAs in terminally differentiated tissues.
Nucleic acids research44(19)9190-9205(2016 Nov)
Lyons SM,Cunningham CH,Welch JD,Groh B,Guo AY,Wei B,Whitfield ML,Xiong Y,Marzluff WF

RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Cell150(6)1182-95(2012 Sep)
Mattiroli F,Vissers JH,van Dijk WJ,Ikpa P,Citterio E,Vermeulen W,Marteijn JA,Sixma TK

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Functional characterization of a human histone gene cluster duplication.
Gene342(1)35-40(2004 Nov)
Braastad CD,Hovhannisyan H,van Wijnen AJ,Stein JL,Stein GS

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H2AC18 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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