LAGE3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LAGE3
Entrez Gene ID	8270
Full Name	L antigen family member 3
Synonyms	CVG5,DXS9879E,DXS9951E,ESO3,GAMOS2,ITBA2,Pcc1
General protein information	Preferred Names L antigen family member 3 Names EKC/KEOPS complex subunit LAGE3 protein ESO-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	This gene belongs to the ESO/LAGE gene family, members of which are clustered together on chromosome Xq28, and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors, this gene is ubiquitously expressed in somatic tissues. The latter, combined with the finding that it is highly conserved in mouse and rat, suggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9. [provided by RefSeq, Jul 2008].
Disorder MIM:	300060
Disorder Html:	Galloway-Mowat syndrome 2, X-linked, 301006 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006014.4	NP_006005.2	EKC/KEOPS complex subunit LAGE3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LAGE3	XP_003435651.2
Canis lupus familiaris (dog)	LOC100856548	XP_003640320.1
Bos taurus (cattle)	LOC782436	XP_002699776.2
Pan troglodytes (chimpanzee)	LAGE3	XP_521343.3
Xenopus tropicalis (tropical clawed frog)	lage3	XP_002937978.1
Homo sapiens (human)	LAGE3	NP_006005.2
	LOC700924	XP_001089237.1
Danio rerio (zebrafish)	LOC100536597	XP_003199438.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Braun DA, Rao J, Mollet G, Schapiro D, Daugeron MC, Tan W, Gribouval O, Boyer O, Revy P, Jobst-Schwan T, Schmidt JM, Lawson JA, Schanze D, Ashraf S, Ullmann JFP, Hoogstraten CA, Boddaert N, Collinet B, Martin G, Liger D, Lovric S, Furlano M, Guerrera IC, Sanchez-Ferras O, Hu JF, Boschat AC, Sanquer S, Menten B, Vergult S, De Rocker N, Airik M, Hermle T, Shril S, Widmeier E, Gee HY, Choi WI, Sadowski CE, Pabst WL, Warejko JK, Daga A, Basta T, Matejas V, Scharmann K, Kienast SD, Behnam B, Beeson B, Begtrup A, Bruce M, Ch'ng GS, Lin SP, Chang JH, Chen CH, Cho MT, Gaffney PM, Gipson PE, Hsu CH, Kari JA, Ke YY, Kiraly-Borri C, Lai WM, Lemyre E, Littlejohn RO, Masri A, Moghtaderi M, Nakamura K, Ozaltin F, Praet M, Prasad C, Prytula A, Roeder ER, Rump P, Schnur RE, Shiihara T, Sinha MD, Soliman NA, Soulami K, Sweetser DA, Tsai WH, Tsai JD, Topaloglu R, Vester U, Viskochil DH, Vatanavicharn N, Waxler JL, Wierenga KJ, Wolf MTF, Wong SN, Leidel SA, Truglio G, Dedon PC, Poduri A, Mane S, Lifton RP, Bouchard M, Kannu P, Chitayat D, Magen D, Callewaert B, van Tilbeurgh H, Zenker M, Antignac C, Hildebrandt F
Nature genetics49(10)1529-1538(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7.
Wan LC, Maisonneuve P, Szilard RK, Lambert JP, Ng TF, Manczyk N, Huang H, Laister R, Caudy AA, Gingras AC, Durocher D, Sicheri F
Nucleic acids research45(2)805-817(2017 Jan)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME.
Costessi A, Mahrour N, Sharma V, Stunnenberg R, Stoel MA, Tijchon E, Conaway JW, Conaway RC, Stunnenberg HG
PloS one7(8)e42822(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LAGE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAGE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu05208	NM_006014.4 Latest version!	Homo sapiens L antigen family member 3 (LAGE3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LAGE3 ( NM_006014.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006005.2 Homo sapiens L antigen family member 3 (LAGE3), mRNA.

Price & Availability↑

CloneID	OHu05208
Clone ID Related Accession (Same CDS sequence)	NM_006014.4
Accession Version	NM_006014.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	EKC/KEOPS complex subunit LAGE3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015744.1 and BQ649305.1. On Sep 12, 2013 this sequence version replaced NM_006014.3. ##Evidence-Data-START## Transcript exon combination :: BC015744.1, SRR1163658.189391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGGACG CGGATGCAGA CGCAGGCGGA GGCGCTGACG GCGGGGATGG CCGGGGTGGC 
CACAGCTGCC GCGGGGGCGT GGACACAGCC GCAGCTCCGG CCGGTGGAGC TCCCCCAGCG 
CACGCGCCAG GTCCGGGCAG AGACGCCGCG TCTGCGGCCA GGGGGTCACG AATGCGGCCG 
CACATATTCA CCCTCAGCGT GCCTTTCCCG ACCCCCTTGG AGGCGGAAAT CGCCCATGGG 
TCCCTGGCAC CAGATGCCGA GCCCCACCAA AGGGTGGTTG GGAAGGATCT CACAGTGAGT 
GGCAGGATCC TGGTCGTCCG CTGGAAAGCT GAAGACTGTC GCCTGCTCCG AATTTCCGTC 
ATCAACTTTC TTGACCAGCT TTCCCTGGTG GTGCGGACCA TGCAGCGCTT TGGGCCCCCC 
GTTTCCCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006005.2
CDS	343..774
Translation	MRDADADAGGGADGGDGRGGHSCRGGVDTAAAPAGGAPPAHAPGPGRDAASAARGSRMRPHIFTLSVPFPTPLEAEIAHGSLAPDAEPHQRVVGKDLTVSGRILVVRWKAEDCRLLRISVINFLDQLSLVVRTMQRFGPPVSR

Target ORF information:

RefSeq Version	NM_006014.4
Organism	Homo sapiens(human)
Definition	Homo sapiens L antigen family member 3 (LAGE3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006014.4

ORF Insert Sequence:

ATGCGGGACG CGGATGCAGA CGCAGGCGGA GGCGCTGACG GCGGGGATGG CCGGGGTGGC 
CACAGCTGCC GCGGGGGCGT GGACACAGCC GCAGCTCCGG CCGGTGGAGC TCCCCCAGCG 
CACGCGCCAG GTCCGGGCAG AGACGCCGCG TCTGCGGCCA GGGGGTCACG AATGCGGCCG 
CACATATTCA CCCTCAGCGT GCCTTTCCCG ACCCCCTTGG AGGCGGAAAT CGCCCATGGG 
TCCCTGGCAC CAGATGCCGA GCCCCACCAA AGGGTGGTTG GGAAGGATCT CACAGTGAGT 
GGCAGGATCC TGGTCGTCCG CTGGAAAGCT GAAGACTGTC GCCTGCTCCG AATTTCCGTC 
ATCAACTTTC TTGACCAGCT TTCCCTGGTG GTGCGGACCA TGCAGCGCTT TGGGCCCCCC 
GTTTCCCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Nature genetics49(10)1529-1538(2017 Oct)
Braun DA,Rao J,Mollet G,Schapiro D,Daugeron MC,Tan W,Gribouval O,Boyer O,Revy P,Jobst-Schwan T,Schmidt JM,Lawson JA,Schanze D,Ashraf S,Ullmann JFP,Hoogstraten CA,Boddaert N,Collinet B,Martin G,Liger D,Lovric S,Furlano M,Guerrera IC,Sanchez-Ferras O,Hu JF,Boschat AC,Sanquer S,Menten B,Vergult S,De Rocker N,Airik M,Hermle T,Shril S,Widmeier E,Gee HY,Choi WI,Sadowski CE,Pabst WL,Warejko JK,Daga A,Basta T,Matejas V,Scharmann K,Kienast SD,Behnam B,Beeson B,Begtrup A,Bruce M,Ch'ng GS,Lin SP,Chang JH,Chen CH,Cho MT,Gaffney PM,Gipson PE,Hsu CH,Kari JA,Ke YY,Kiraly-Borri C,Lai WM,Lemyre E,Littlejohn RO,Masri A,Moghtaderi M,Nakamura K,Ozaltin F,Praet M,Prasad C,Prytula A,Roeder ER,Rump P,Schnur RE,Shiihara T,Sinha MD,Soliman NA,Soulami K,Sweetser DA,Tsai WH,Tsai JD,Topaloglu R,Vester U,Viskochil DH,Vatanavicharn N,Waxler JL,Wierenga KJ,Wolf MTF,Wong SN,Leidel SA,Truglio G,Dedon PC,Poduri A,Mane S,Lifton RP,Bouchard M,Kannu P,Chitayat D,Magen D,Callewaert B,van Tilbeurgh H,Zenker M,Antignac C,Hildebrandt F

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7.
Nucleic acids research45(2)805-817(2017 Jan)
Wan LC,Maisonneuve P,Szilard RK,Lambert JP,Ng TF,Manczyk N,Huang H,Laister R,Caudy AA,Gingras AC,Durocher D,Sicheri F

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME.
PloS one7(8)e42822(2012)
Costessi A,Mahrour N,Sharma V,Stunnenberg R,Stoel MA,Tijchon E,Conaway JW,Conaway RC,Stunnenberg HG

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LAGE3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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