KIF18A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIF18A
Entrez Gene ID	81930
Full Name	kinesin family member 18A
Synonyms	MS-KIF18A,PPP1R99
General protein information	Preferred Names kinesin family member 18A Names kinesin-like protein KIF18A protein phosphatase 1, regulatory subunit 99
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p14.1
Summary	KIF18A is a member of the kinesin superfamily of microtubule-associated molecular motors (see MIM 148760) that use hydrolysis of ATP to produce force and movement along microtubules (Luboshits and Benayahu, 2005 [PubMed 15878648]).[supplied by OMIM, Aug 2008].
Disorder MIM:	611271

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018379.1	XP_016873868.1	kinesin-like protein KIF18A isoform X1
XM_017018380.1	XP_016873869.1	kinesin-like protein KIF18A isoform X2
NM_031217.3	NP_112494.3	kinesin-like protein KIF18A
NM_031217.4	NP_112494.3	kinesin-like protein KIF18A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Kif18a	NP_647464.1
Xenopus tropicalis (tropical clawed frog)	kif18a	NP_001016245.2
Homo sapiens (human)	KIF18A	NP_112494.3
Canis lupus familiaris (dog)	KIF18A	XP_005633821.1
Bos taurus (cattle)	KIF18A	NP_001179838.1
Danio rerio (zebrafish)	kif18a	NP_956533.1
Pan troglodytes (chimpanzee)	KIF18A	XP_001138250.1
	KIF18A	XP_001089262.1
Rattus norvegicus (Norway rat)	Kif18a	NP_001131114.1
Gallus gallus (chicken)	KIF18A	NP_001186355.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Exclusive destruction of mitotic spindles in human cancer cells.
Visochek L, Castiel A, Mittelman L, Elkin M, Atias D, Golan T, Izraeli S, Peretz T, Cohen-Armon M
Oncotarget8(13)20813-20824(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low post translational modifications of KIF18A protein is associated with neoplasms.
Title: Exclusive destruction of mitotic spindles in human cancer cells.

KIF18a role in microtubule assembly
Title: Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins.

Confocal microscopy shows that cells expressing SUMO-resistant Kif18A display a compromised dissociation of BubR1 from kinetochores after anaphase onset.
Title: Sumoylation of Kif18A plays a role in regulating mitotic progression.

a biomarker for hepatocellular carcinoma diagnosis and an independent predictor of overall survival
Title: High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma.

that the human mitotic kinesin-8, KIF18A, directly interacts with PP1gamma through a conserved RVxF motif
Title: The human mitotic kinesin KIF18A binds protein phosphatase 1 (PP1) through a highly conserved docking motif.

The following KIF18A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIF18A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu92558	XM_017018380.1 Latest version!	Homo sapiens kinesin family member 18A (KIF18A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu28001	NM_031217.4 Latest version!	Homo sapiens kinesin family member 18A (KIF18A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28001	XM_017018379.1 Latest version!	Homo sapiens kinesin family member 18A (KIF18A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu28001	NM_031217.3	Homo sapiens kinesin family member 18A (KIF18A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIF18A ( XM_017018380.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873869.1 Homo sapiens kinesin family member 18A (KIF18A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92558
Clone ID Related Accession (Same CDS sequence)	XM_017018380.1
Accession Version	XM_017018380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinesin-like protein KIF18A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACTTA AAGAAAATGA ACTTAAATCA TTCTACCAAC AACAGTGCCA TAAACAAATA 
GAAATGATGT GTTCTGAAGA CAAAGTAGAA AAGGCCACTG GAAAACGAGA TCATAGACTT 
GCAATGTTGA AAACTCGTCG CTCCTACCTG GAGAAAAGGA GGGAGGAGGA ATTGAAGCAA 
TTTGATGAGA ATACTAATTG GCTCCATCGT GTCGAAAAAG AAATGGGACT CTTAAGTCAA 
AACGGTCATA TTCCAAAGGA ACTCAAGAAA GATCTTCATT GTCACCATTT GCACCTCCAG 
AACAAAGATT TGAAAGCACA AATTAGACAT ATGATGGATC TAGCTTGTCT TCAGGAACAG 
CAACACAGGC AGACTGAAGC AGTATTGAAT GCTTTACTTC CAACCCTAAG AAAACAATAT 
TGCACATTAA AAGAAGCCGG CCTGTCAAAT GCTGCTTTTG AATCTGACTT CAAAGAGATC 
GAACATTTGG TAGAGAGGAA AAAAGTGGTA GTTTGGGCTG ACCAAACTGC CGAACAACCA 
AAGCAAAACG ATCTACCAGG GATTTCTGTT CTTATGACCT TTCCACAACT TGGACCAGTT 
CAGCCTATTC CTTGTTGCTC ATCTTCAGGT GGAACTAATC TGGTTAAGAT TCCTACAGAA 
AAAAGAACTC GGAGAAAACT AATGCCATCT CCCTTGAAAG GACAGCATAC TCTAAAGTCT 
CCACCATCTC AAAGTGTGCA GCTCAATGAT TCTCTTAGCA AAGAACTTCA GCCTATTGTA 
TATACACCAG AAGACTGTAG AAAAGCTTTT CAAAATCCGT CTACAGTAAC CTTAATGAAA 
CCATCATCAT TTACTACAAG TTTTCAGGCT ATCAGCTCAA ACATAAACAG TGATAATTGT 
CTGAAAATGT TGTGTGAAGT AGCTATCCCT CATAATAGAA GAAAAGAATG TGGACAGGAG 
GACTTGGACT CTACATTTAC TATATGTGAA GACATCAAGA GCTCGAAGTG TAAATTACCC 
GAACAAGAAT CACTACCAAA TGATAACAAA GACATTTTAC AACGGCTTGA TCCTTCTTCA 
TTCTCAACTA AGCATTCTAT GCCTGTACCA AGCATGGTGC CATCCTACAT GGCAATGACT 
ACTGCTGCCA AAAGGAAACG GAAATTAACA AGTTCTACAT CAAACAGTTC GTTAACTGCA 
GACGTAAATT CTGGATTTGC CAAACGTGTT CGACAAGATA ATTCAAGTGA GAAGCACTTA 
CAAGAAAACA AACCAACAAT GGAACATAAA AGAAACATCT GTAAAATAAA TCCAAGCATG 
GTTAGAAAAT TTGGAAGAAA TATTTCAAAA GGAAATCTAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873869.1
CDS	223..1587
Translation	MLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Target ORF information:

RefSeq Version	XM_017018380.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinesin family member 18A (KIF18A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018380.1

ORF Insert Sequence:

ATGTTACTTA AAGAAAATGA ACTTAAATCA TTCTACCAAC AACAGTGCCA TAAACAAATA 
GAAATGATGT GTTCTGAAGA CAAAGTAGAA AAGGCCACTG GAAAACGAGA TCATAGACTT 
GCAATGTTGA AAACTCGTCG CTCCTACCTG GAGAAAAGGA GGGAGGAGGA ATTGAAGCAA 
TTTGATGAGA ATACTAATTG GCTCCATCGT GTCGAAAAAG AAATGGGACT CTTAAGTCAA 
AACGGTCATA TTCCAAAGGA ACTCAAGAAA GATCTTCATT GTCACCATTT GCACCTCCAG 
AACAAAGATT TGAAAGCACA AATTAGACAT ATGATGGATC TAGCTTGTCT TCAGGAACAG 
CAACACAGGC AGACTGAAGC AGTATTGAAT GCTTTACTTC CAACCCTAAG AAAACAATAT 
TGCACATTAA AAGAAGCCGG CCTGTCAAAT GCTGCTTTTG AATCTGACTT CAAAGAGATC 
GAACATTTGG TAGAGAGGAA AAAAGTGGTA GTTTGGGCTG ACCAAACTGC CGAACAACCA 
AAGCAAAACG ATCTACCAGG GATTTCTGTT CTTATGACCT TTCCACAACT TGGACCAGTT 
CAGCCTATTC CTTGTTGCTC ATCTTCAGGT GGAACTAATC TGGTTAAGAT TCCTACAGAA 
AAAAGAACTC GGAGAAAACT AATGCCATCT CCCTTGAAAG GACAGCATAC TCTAAAGTCT 
CCACCATCTC AAAGTGTGCA GCTCAATGAT TCTCTTAGCA AAGAACTTCA GCCTATTGTA 
TATACACCAG AAGACTGTAG AAAAGCTTTT CAAAATCCGT CTACAGTAAC CTTAATGAAA 
CCATCATCAT TTACTACAAG TTTTCAGGCT ATCAGCTCAA ACATAAACAG TGATAATTGT 
CTGAAAATGT TGTGTGAAGT AGCTATCCCT CATAATAGAA GAAAAGAATG TGGACAGGAG 
GACTTGGACT CTACATTTAC TATATGTGAA GACATCAAGA GCTCGAAGTG TAAATTACCC 
GAACAAGAAT CACTACCAAA TGATAACAAA GACATTTTAC AACGGCTTGA TCCTTCTTCA 
TTCTCAACTA AGCATTCTAT GCCTGTACCA AGCATGGTGC CATCCTACAT GGCAATGACT 
ACTGCTGCCA AAAGGAAACG GAAATTAACA AGTTCTACAT CAAACAGTTC GTTAACTGCA 
GACGTAAATT CTGGATTTGC CAAACGTGTT CGACAAGATA ATTCAAGTGA GAAGCACTTA 
CAAGAAAACA AACCAACAAT GGAACATAAA AGAAACATCT GTAAAATAAA TCCAAGCATG 
GTTAGAAAAT TTGGAAGAAA TATTTCAAAA GGAAATCTAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF18A ( NM_031217.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_112494.3 Homo sapiens kinesin family member 18A (KIF18A), mRNA.

Price & Availability↑

CloneID	OHu28001
Clone ID Related Accession (Same CDS sequence)	XM_017018379.1 , NM_031217.3 , NM_031217.4
Accession Version	NM_031217.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	kinesin-like protein KIF18A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB080843.1, AL136819.1 and AW770226.1. On Jun 6, 2007 this sequence version replaced NM_031217.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY791349.1, AB062483.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112494.3
CDS	183..2879
Translation	MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIERFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Target ORF information:

RefSeq Version	NM_031217.3
Organism	Homo sapiens(human)
Definition	Homo sapiens kinesin family member 18A (KIF18A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031217.3

ORF Insert Sequence:

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF18A ( NM_031217.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_112494.3 Homo sapiens kinesin family member 18A (KIF18A), mRNA.

Price & Availability↑

CloneID	OHu28001
Clone ID Related Accession (Same CDS sequence)	XM_017018379.1 , NM_031217.3 , NM_031217.4
Accession Version	NM_031217.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	kinesin-like protein KIF18A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB080843.1, AL136819.1 and AW770226.1. On Nov 23, 2018 this sequence version replaced NM_031217.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY791349.1, AL136819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263181.7/ ENSP00000263181.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112494.3
CDS	140..2836
Translation	MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIERFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Target ORF information:

RefSeq Version	NM_031217.4
Organism	Homo sapiens(human)
Definition	Homo sapiens kinesin family member 18A (KIF18A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031217.4

ORF Insert Sequence:

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF18A ( XM_017018379.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873868.1 Homo sapiens kinesin family member 18A (KIF18A), transcript variant X1, mRNA.

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CloneID	OHu28001
Clone ID Related Accession (Same CDS sequence)	XM_017018379.1 , NM_031217.3 , NM_031217.4
Accession Version	XM_017018379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinesin-like protein KIF18A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873868.1
CDS	739..3435
Translation	MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIERFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Target ORF information:

RefSeq Version	XM_017018379.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinesin family member 18A (KIF18A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018379.1

ORF Insert Sequence:

ATGTCTGTCA CTGAGGAAGA CCTGTGCCAC CATATGAAAG TAGTAGTTCG TGTACGTCCG 
GAAAACACTA AAGAAAAAGC AGCTGGATTT CATAAAGTGG TTCATGTTGT GGATAAACAT 
ATCCTAGTTT TTGATCCCAA ACAAGAAGAA GTCAGTTTTT TCCATGGAAA GAAAACTACA 
AATCAAAATG TTATAAAGAA ACAAAATAAG GATCTTAAAT TTGTATTTGA TGCTGTTTTT 
GATGAAACGT CAACTCAGTC AGAAGTTTTT GAACACACTA CTAAGCCAAT TCTTCGTAGT 
TTTTTGAATG GATATAATTG CACAGTACTT GCCTATGGTG CCACTGGTGC TGGGAAGACC 
CACACTATGC TAGGATCAGC TGATGAACCT GGAGTGATGT ATCTAACAAT GTTACACCTT 
TACAAATGCA TGGATGAGAT TAAAGAAGAG AAAATATGTA GTACTGCAGT TTCATATCTG 
GAGGTATATA ATGAACAGAT TCGTGATCTC TTAGTAAATT CAGGGCCACT TGCTGTCCGG 
GAAGATACCC AAAAAGGGGT GGTCGTTCAT GGACTTACTT TACACCAGCC CAAATCCTCA 
GAAGAAATTT TACATTTATT GGATAATGGA AACAAAAACA GGACACAACA TCCCACTGAT 
ATGAATGCCA CATCTTCTCG TTCTCATGCT GTTTTCCAAA TTTACTTGCG ACAACAAGAC 
AAAACAGCAA GTATCAATCA AAATGTCCGT ATTGCCAAGA TGTCACTCAT TGACCTGGCA 
GGATCTGAGC GAGCAAGTAC TTCCGGTGCT AAGGGGACCC GATTTGTAGA AGGCACAAAT 
ATTAATAGAT CACTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAGA TTCAAAGAGA 
AAGAATCAGC ATATCCCTTA CAGAAATAGT AAGCTTACTC GCTTGTTAAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTGT ATTCTACGAT 
GACACATATA ACACTCTTAA GTATGCTAAC CGGGCAAAGG ACATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATGTCAA TAATCATATA ACTCAATATG TAAAGATCTG TAATGAGCAG 
AAGGCAGAGA TTTTATTGTT AAAAGAAAAA CTAAAAGCCT ATGAAGAACA GAAAGCCTTC 
ACTAATGAAA ATGACCAAGC AAAGTTAATG ATTTCAAACC CTCAGGAAAA AGAAATCGAA 
AGGTTTCAAG AAATCCTGAA CTGCTTGTTC CAGAATCGAG AAGAAATTAG ACAAGAATAT 
CTGAAGTTGG AAATGTTACT TAAAGAAAAT GAACTTAAAT CATTCTACCA ACAACAGTGC 
CATAAACAAA TAGAAATGAT GTGTTCTGAA GACAAAGTAG AAAAGGCCAC TGGAAAACGA 
GATCATAGAC TTGCAATGTT GAAAACTCGT CGCTCCTACC TGGAGAAAAG GAGGGAGGAG 
GAATTGAAGC AATTTGATGA GAATACTAAT TGGCTCCATC GTGTCGAAAA AGAAATGGGA 
CTCTTAAGTC AAAACGGTCA TATTCCAAAG GAACTCAAGA AAGATCTTCA TTGTCACCAT 
TTGCACCTCC AGAACAAAGA TTTGAAAGCA CAAATTAGAC ATATGATGGA TCTAGCTTGT 
CTTCAGGAAC AGCAACACAG GCAGACTGAA GCAGTATTGA ATGCTTTACT TCCAACCCTA 
AGAAAACAAT ATTGCACATT AAAAGAAGCC GGCCTGTCAA ATGCTGCTTT TGAATCTGAC 
TTCAAAGAGA TCGAACATTT GGTAGAGAGG AAAAAAGTGG TAGTTTGGGC TGACCAAACT 
GCCGAACAAC CAAAGCAAAA CGATCTACCA GGGATTTCTG TTCTTATGAC CTTTCCACAA 
CTTGGACCAG TTCAGCCTAT TCCTTGTTGC TCATCTTCAG GTGGAACTAA TCTGGTTAAG 
ATTCCTACAG AAAAAAGAAC TCGGAGAAAA CTAATGCCAT CTCCCTTGAA AGGACAGCAT 
ACTCTAAAGT CTCCACCATC TCAAAGTGTG CAGCTCAATG ATTCTCTTAG CAAAGAACTT 
CAGCCTATTG TATATACACC AGAAGACTGT AGAAAAGCTT TTCAAAATCC GTCTACAGTA 
ACCTTAATGA AACCATCATC ATTTACTACA AGTTTTCAGG CTATCAGCTC AAACATAAAC 
AGTGATAATT GTCTGAAAAT GTTGTGTGAA GTAGCTATCC CTCATAATAG AAGAAAAGAA 
TGTGGACAGG AGGACTTGGA CTCTACATTT ACTATATGTG AAGACATCAA GAGCTCGAAG 
TGTAAATTAC CCGAACAAGA ATCACTACCA AATGATAACA AAGACATTTT ACAACGGCTT 
GATCCTTCTT CATTCTCAAC TAAGCATTCT ATGCCTGTAC CAAGCATGGT GCCATCCTAC 
ATGGCAATGA CTACTGCTGC CAAAAGGAAA CGGAAATTAA CAAGTTCTAC ATCAAACAGT 
TCGTTAACTG CAGACGTAAA TTCTGGATTT GCCAAACGTG TTCGACAAGA TAATTCAAGT 
GAGAAGCACT TACAAGAAAA CAAACCAACA ATGGAACATA AAAGAAACAT CTGTAAAATA 
AATCCAAGCA TGGTTAGAAA ATTTGGAAGA AATATTTCAA AAGGAAATCT AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Exclusive destruction of mitotic spindles in human cancer cells.
Oncotarget8(13)20813-20824(2017 Mar)
Visochek L,Castiel A,Mittelman L,Elkin M,Atias D,Golan T,Izraeli S,Peretz T,Cohen-Armon M

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KIF18A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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