SPACA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPACA1
Entrez Gene ID	81833
Full Name	sperm acrosome associated 1
Synonyms	SAMP32
General protein information	Preferred Names sperm acrosome associated 1 Names sperm acrosome membrane-associated protein 1 sperm acrosomal membrane-associated protein 32
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q15
Summary	The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively. [provided by RefSeq, Jul 2008].
Disorder MIM:	612739

mRNA and Protein(s)

mRNA	Protein	Name
NM_030960.2	NP_112222.1	sperm acrosome membrane-associated protein 1 precursor
XM_011536160.2	XP_011534462.1	sperm acrosome membrane-associated protein 1 isoform X1
NM_030960.3	NP_112222.1	sperm acrosome membrane-associated protein 1 precursor
XM_017011335.1	XP_016866824.1	sperm acrosome membrane-associated protein 1 isoform X1

Pathways from BioSystems

Homologies

	SPACA1	XP_001093826.1
Canis lupus familiaris (dog)	SPACA1	XP_854415.1
Bos taurus (cattle)	SPACA1	NP_001040010.1
Homo sapiens (human)	SPACA1	NP_112222.1
Pan troglodytes (chimpanzee)	SPACA1	XP_518632.2
Mus musculus (house mouse)	Spaca1	NP_080569.1
Rattus norvegicus (Norway rat)	Spaca1	NP_001178911.1
Gallus gallus (chicken)	SPACA1	XP_419844.2

Gene Ontology
Bibliography

Related articles in PubMed

Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization.
Kishida K, Harayama H, Kimura F, Murakami T
Zygote (Cambridge, England)24(5)654-61(2016 Oct)

Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.
Chen X, Liu X, Ren X, Li X, Wang L, Zang W
Reproduction, fertility, and development28(4)416-27(2016 Mar)

L-type voltage-dependent calcium channel alpha subunit 1C is a novel candidate gene associated with secondary hyperparathyroidism: an application of haplotype-based analysis for multiple linked single nucleotide polymorphisms.
Yokoyama K, Urashima M, Ohkido I, Kono T, Yoshida T, Muramatsu M, Niu T, Hosoya T
Nephron. Clinical practice115(4)c237-43(2010)

Genome-wide association of echocardiographic dimensions, brachial artery endothelial function and treadmill exercise responses in the Framingham Heart Study.
Vasan RS, Larson MG, Aragam J, Wang TJ, Mitchell GF, Kathiresan S, Newton-Cheh C, Vita JA, Keyes MJ, O'Donnell CJ, Levy D, Benjamin EJ
BMC medical genetics8 Suppl 1S2(2007 Sep)

The DNA sequence and analysis of human chromosome 6.
Mungall AJ, Palmer SA, Sims SK, Edwards CA, Ashurst JL, Wilming L, Jones MC, Horton R, Hunt SE, Scott CE, Gilbert JG, Clamp ME, Bethel G, Milne S, Ainscough R, Almeida JP, Ambrose KD, Andrews TD, Ashwell RI, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beare DM, Beasley H, Beasley O, Bird CP, Blakey S, Bray-Allen S, Brook J, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Carder C, Carter NP, Chapman JC, Clark SY, Clark G, Clee CM, Clegg S, Cobley V, Collier RE, Collins JE, Colman LK, Corby NR, Coville GJ, Culley KM, Dhami P, Davies J, Dunn M, Earthrowl ME, Ellington AE, Evans KA, Faulkner L, Francis MD, Frankish A, Frankland J, French L, Garner P, Garnett J, Ghori MJ, Gilby LM, Gillson CJ, Glithero RJ, Grafham DV, Grant M, Gribble S, Griffiths C, Griffiths M, Hall R, Halls KS, Hammond S, Harley JL, Hart EA, Heath PD, Heathcott R, Holmes SJ, Howden PJ, Howe KL, Howell GR, Huckle E, Humphray SJ, Humphries MD, Hunt AR, Johnson CM, Joy AA, Kay M, Keenan SJ, Kimberley AM, King A, Laird GK, Langford C, Lawlor S, Leongamornlert DA, Leversha M, Lloyd CR, Lloyd DM, Loveland JE, Lovell J, Martin S, Mashreghi-Mohammadi M, Maslen GL, Matthews L, McCann OT, McLaren SJ, McLay K, McMurray A, Moore MJ, Mullikin JC, Niblett D, Nickerson T, Novik KL, Oliver K, Overton-Larty EK, Parker A, Patel R, Pearce AV, Peck AI, Phillimore B, Phillips S, Plumb RW, Porter KM, Ramsey Y, Ranby SA, Rice CM, Ross MT, Searle SM, Sehra HK, Sheridan E, Skuce CD, Smith S, Smith M, Spraggon L, Squares SL, Steward CA, Sycamore N, Tamlyn-Hall G, Tester J, Theaker AJ, Thomas DW, Thorpe A, Tracey A, Tromans A, Tubby B, Wall M, Wallis JM, West AP, White SS, Whitehead SL, Whittaker H, Wild A, Willey DJ, Wilmer TE, Wood JM, Wray PW, Wyatt JC, Young L, Younger RM, Bentley DR, Coulson A, Durbin R, Hubbard T, Sulston JE, Dunham I, Rogers J, Beck S
Nature425(6960)805-11(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spaca1 role in male infertility.Distribution of SPACA1 in the sperm affects the outcomes of conventional in vitro fertilization.
Title: Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization.

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice.
Title: Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.

Observational study of gene-disease association. (HuGE Navigator)
Title: L-type voltage-dependent calcium channel alpha subunit 1C is a novel candidate gene associated with secondary hyperparathyroidism: an application of haplotype-based analysis for multiple linked single nucleotide polymorphisms.

SAMP32 was associated with the inner acrosomal membrane and recombinant SAMP32 reacted with serum from an infertile man suggesting that it is isoantigenic
Title: SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein.

The following SPACA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPACA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32222	NM_030960.3 Latest version!	Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu72996	XM_017011335.1 Latest version!	Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu72996	XM_011536160.2 Latest version!	Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OHu32222	NM_030960.2	Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPACA1 ( NM_030960.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_112222.1 Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.

Price & Availability↑

CloneID	OHu32222
Clone ID Related Accession (Same CDS sequence)	NM_030960.2 , NM_030960.3
Accession Version	NM_030960.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	sperm acrosome membrane-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC029488.1, AF203447.1 and AL136096.7. On Sep 18, 2002 this sequence version replaced NM_030960.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF203447.1, BI829277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCCA GGGGCACGGG CTGCTCCGCC GGGCTGCTGA TGACTGTCGG CTGGCTGCTT 
CTGGCGGGCC TCCAGTCCGC GCGCGGGACC AACGTCACCG CTGCCGTCCA GGATGCCGGC 
CTGGCCCACG AAGGCGAGGG CGAGGAGGAG ACCGAAAACA ACGACAGCGA GACCGCGGAG 
AACTACGCTC CGCCTGAAAC CGAGGATGTT TCAAATAGGA ATGTCGTCAA AGAAGTAGAA 
TTCGGAATGT GCACCGTTAC ATGTGGTATT GGTGTTAGAG AAGTTATATT AACAAATGGA 
TGCCCTGGTG GTGAATCCAA GTGTGTTGTA CGGGTAGAAG AATGCCGTGG ACCAACAGAT 
TGTGGCTGGG GTAAACCAAT TTCAGAAAGT CTTGAAAGTG TTAGATTGGC ATGTATTCAC 
ACATCTCCCT TAAATCGTTT CAAATATATG TGGAAACTTC TAAGACAAGA CCAACAATCC 
ATTATACTTG TAAATGATTC AGCAATCCTA GAAGTACGCA AGGAAAGTCA CCCCTTGGCT 
TTCGAGTGTG ACACACTGGA TAATAATGAA ATAGTAGCAA CTATTAAATT CACAGTCTAT 
ACGAGCAGTG AATTGCAGAT GAGAAGATCA AGCCTACCAG CCACTGATGC AGCCCTAATT 
TTTGTGCTGA CCATAGGAGT CATTATCTGT GTATTTATAA TTTTCTTATT GATCTTCATA 
ATCATAAATT GGGCAGCAGT CAAGGCTTTC TGGGGGGCAA AAGCCTCTAC ACCTGAGGTA 
CAATCCGAGC AGAGTTCTGT GAGATACAAA GATTCAACTT CTCTTGACCA ATTACCAACA 
GAAATGCCTG GTGAAGATGA TGCTTTAAGT GAATGGAATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112222.1
CDS	118..1002
Translation	MSPRGTGCSAGLLMTVGWLLLAGLQSARGTNVTAAVQDAGLAHEGEGEEETENNDSETAENYAPPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSLDQLPTEMPGEDDALSEWNE

Target ORF information:

RefSeq Version	NM_030960.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030960.2

ORF Insert Sequence:

ATGAGCCCCA GGGGCACGGG CTGCTCCGCC GGGCTGCTGA TGACTGTCGG CTGGCTGCTT 
CTGGCGGGCC TCCAGTCCGC GCGCGGGACC AACGTCACCG CTGCCGTCCA GGATGCCGGC 
CTGGCCCACG AAGGCGAGGG CGAGGAGGAG ACCGAAAACA ACGACAGCGA GACCGCGGAG 
AACTACGCTC CGCCTGAAAC CGAGGATGTT TCAAATAGGA ATGTCGTCAA AGAAGTAGAA 
TTCGGAATGT GCACCGTTAC ATGTGGTATT GGTGTTAGAG AAGTTATATT AACAAATGGA 
TGCCCTGGTG GTGAATCCAA GTGTGTTGTA CGGGTAGAAG AATGCCGTGG ACCAACAGAT 
TGTGGCTGGG GTAAACCAAT TTCAGAAAGT CTTGAAAGTG TTAGATTGGC ATGTATTCAC 
ACATCTCCCT TAAATCGTTT CAAATATATG TGGAAACTTC TAAGACAAGA CCAACAATCC 
ATTATACTTG TAAATGATTC AGCAATCCTA GAAGTACGCA AGGAAAGTCA CCCCTTGGCT 
TTCGAGTGTG ACACACTGGA TAATAATGAA ATAGTAGCAA CTATTAAATT CACAGTCTAT 
ACGAGCAGTG AATTGCAGAT GAGAAGATCA AGCCTACCAG CCACTGATGC AGCCCTAATT 
TTTGTGCTGA CCATAGGAGT CATTATCTGT GTATTTATAA TTTTCTTATT GATCTTCATA 
ATCATAAATT GGGCAGCAGT CAAGGCTTTC TGGGGGGCAA AAGCCTCTAC ACCTGAGGTA 
CAATCCGAGC AGAGTTCTGT GAGATACAAA GATTCAACTT CTCTTGACCA ATTACCAACA 
GAAATGCCTG GTGAAGATGA TGCTTTAAGT GAATGGAATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPACA1 ( NM_030960.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_112222.1 Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.

Price & Availability↑

CloneID	OHu32222
Clone ID Related Accession (Same CDS sequence)	NM_030960.2 , NM_030960.3
Accession Version	NM_030960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	sperm acrosome membrane-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF203447.1, BC029488.1 and AL136096.7. On Nov 23, 2018 this sequence version replaced NM_030960.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF203447.1, BC029488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000237201.2/ ENSP00000237201.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCCCCA GGGGCACGGG CTGCTCCGCC GGGCTGCTGA TGACTGTCGG CTGGCTGCTT 
CTGGCGGGCC TCCAGTCCGC GCGCGGGACC AACGTCACCG CTGCCGTCCA GGATGCCGGC 
CTGGCCCACG AAGGCGAGGG CGAGGAGGAG ACCGAAAACA ACGACAGCGA GACCGCGGAG 
AACTACGCTC CGCCTGAAAC CGAGGATGTT TCAAATAGGA ATGTCGTCAA AGAAGTAGAA 
TTCGGAATGT GCACCGTTAC ATGTGGTATT GGTGTTAGAG AAGTTATATT AACAAATGGA 
TGCCCTGGTG GTGAATCCAA GTGTGTTGTA CGGGTAGAAG AATGCCGTGG ACCAACAGAT 
TGTGGCTGGG GTAAACCAAT TTCAGAAAGT CTTGAAAGTG TTAGATTGGC ATGTATTCAC 
ACATCTCCCT TAAATCGTTT CAAATATATG TGGAAACTTC TAAGACAAGA CCAACAATCC 
ATTATACTTG TAAATGATTC AGCAATCCTA GAAGTACGCA AGGAAAGTCA CCCCTTGGCT 
TTCGAGTGTG ACACACTGGA TAATAATGAA ATAGTAGCAA CTATTAAATT CACAGTCTAT 
ACGAGCAGTG AATTGCAGAT GAGAAGATCA AGCCTACCAG CCACTGATGC AGCCCTAATT 
TTTGTGCTGA CCATAGGAGT CATTATCTGT GTATTTATAA TTTTCTTATT GATCTTCATA 
ATCATAAATT GGGCAGCAGT CAAGGCTTTC TGGGGGGCAA AAGCCTCTAC ACCTGAGGTA 
CAATCCGAGC AGAGTTCTGT GAGATACAAA GATTCAACTT CTCTTGACCA ATTACCAACA 
GAAATGCCTG GTGAAGATGA TGCTTTAAGT GAATGGAATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112222.1
CDS	66..950
Translation	MSPRGTGCSAGLLMTVGWLLLAGLQSARGTNVTAAVQDAGLAHEGEGEEETENNDSETAENYAPPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSLDQLPTEMPGEDDALSEWNE

Target ORF information:

RefSeq Version	NM_030960.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm acrosome associated 1 (SPACA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030960.3

ORF Insert Sequence:

ATGAGCCCCA GGGGCACGGG CTGCTCCGCC GGGCTGCTGA TGACTGTCGG CTGGCTGCTT 
CTGGCGGGCC TCCAGTCCGC GCGCGGGACC AACGTCACCG CTGCCGTCCA GGATGCCGGC 
CTGGCCCACG AAGGCGAGGG CGAGGAGGAG ACCGAAAACA ACGACAGCGA GACCGCGGAG 
AACTACGCTC CGCCTGAAAC CGAGGATGTT TCAAATAGGA ATGTCGTCAA AGAAGTAGAA 
TTCGGAATGT GCACCGTTAC ATGTGGTATT GGTGTTAGAG AAGTTATATT AACAAATGGA 
TGCCCTGGTG GTGAATCCAA GTGTGTTGTA CGGGTAGAAG AATGCCGTGG ACCAACAGAT 
TGTGGCTGGG GTAAACCAAT TTCAGAAAGT CTTGAAAGTG TTAGATTGGC ATGTATTCAC 
ACATCTCCCT TAAATCGTTT CAAATATATG TGGAAACTTC TAAGACAAGA CCAACAATCC 
ATTATACTTG TAAATGATTC AGCAATCCTA GAAGTACGCA AGGAAAGTCA CCCCTTGGCT 
TTCGAGTGTG ACACACTGGA TAATAATGAA ATAGTAGCAA CTATTAAATT CACAGTCTAT 
ACGAGCAGTG AATTGCAGAT GAGAAGATCA AGCCTACCAG CCACTGATGC AGCCCTAATT 
TTTGTGCTGA CCATAGGAGT CATTATCTGT GTATTTATAA TTTTCTTATT GATCTTCATA 
ATCATAAATT GGGCAGCAGT CAAGGCTTTC TGGGGGGCAA AAGCCTCTAC ACCTGAGGTA 
CAATCCGAGC AGAGTTCTGT GAGATACAAA GATTCAACTT CTCTTGACCA ATTACCAACA 
GAAATGCCTG GTGAAGATGA TGCTTTAAGT GAATGGAATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPACA1 ( XM_011536160.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534462.1 Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu72996
Clone ID Related Accession (Same CDS sequence)	XM_011536160.2 , XM_017011335.1
Accession Version	XM_011536160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm acrosome membrane-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCG TTACATGTGG TATTGGTGTT AGAGAAGTTA TATTAACAAA TGGATGCCCT 
GGTGGTGAAT CCAAGTGTGT TGTACGGGTA GAAGAATGCC GTGGACCAAC AGATTGTGGC 
TGGGGTAAAC CAATTTCAGA AAGTCTTGAA AGTGTTAGAT TGGCATGTAT TCACACATCT 
CCCTTAAATC GTTTCAAATA TATGTGGAAA CTTCTAAGAC AAGACCAACA ATCCATTATA 
CTTGTAAATG ATTCAGCAAT CCTAGAAGTA CGCAAGGAAA GTCACCCCTT GGCTTTCGAG 
TGTGACACAC TGGATAATAA TGAAATAGTA GCAACTATTA AATTCACAGT CTATACGAGC 
AGTGAATTGC AGATGAGAAG ATCAAGCCTA CCAGCCACTG ATGCAGCCCT AATTTTTGTG 
CTGACCATAG GAGTCATTAT CTGTGTATTT ATAATTTTCT TATTGATCTT CATAATCATA 
AATTGGGCAG CAGTCAAGGC TTTCTGGGGG GCAAAAGCCT CTACACCTGA GGTACAATCC 
GAGCAGAGTT CTGTGAGATA CAAAGATTCA ACTTCTCTTG ACCAATTACC AACAGAAATG 
CCTGGTGAAG ATGATGCTTT AAGTGAATGG AATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534462.1
CDS	318..956
Translation	MCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSLDQLPTEMPGEDDALSEWNE

Target ORF information:

RefSeq Version	XM_011536160.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536160.2

ORF Insert Sequence:

ATGTGCACCG TTACATGTGG TATTGGTGTT AGAGAAGTTA TATTAACAAA TGGATGCCCT 
GGTGGTGAAT CCAAGTGTGT TGTACGGGTA GAAGAATGCC GTGGACCAAC AGATTGTGGC 
TGGGGTAAAC CAATTTCAGA AAGTCTTGAA AGTGTTAGAT TGGCATGTAT TCACACATCT 
CCCTTAAATC GTTTCAAATA TATGTGGAAA CTTCTAAGAC AAGACCAACA ATCCATTATA 
CTTGTAAATG ATTCAGCAAT CCTAGAAGTA CGCAAGGAAA GTCACCCCTT GGCTTTCGAG 
TGTGACACAC TGGATAATAA TGAAATAGTA GCAACTATTA AATTCACAGT CTATACGAGC 
AGTGAATTGC AGATGAGAAG ATCAAGCCTA CCAGCCACTG ATGCAGCCCT AATTTTTGTG 
CTGACCATAG GAGTCATTAT CTGTGTATTT ATAATTTTCT TATTGATCTT CATAATCATA 
AATTGGGCAG CAGTCAAGGC TTTCTGGGGG GCAAAAGCCT CTACACCTGA GGTACAATCC 
GAGCAGAGTT CTGTGAGATA CAAAGATTCA ACTTCTCTTG ACCAATTACC AACAGAAATG 
CCTGGTGAAG ATGATGCTTT AAGTGAATGG AATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPACA1 ( XM_017011335.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866824.1 Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu72996
Clone ID Related Accession (Same CDS sequence)	XM_011536160.2 , XM_017011335.1
Accession Version	XM_017011335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm acrosome membrane-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCG TTACATGTGG TATTGGTGTT AGAGAAGTTA TATTAACAAA TGGATGCCCT 
GGTGGTGAAT CCAAGTGTGT TGTACGGGTA GAAGAATGCC GTGGACCAAC AGATTGTGGC 
TGGGGTAAAC CAATTTCAGA AAGTCTTGAA AGTGTTAGAT TGGCATGTAT TCACACATCT 
CCCTTAAATC GTTTCAAATA TATGTGGAAA CTTCTAAGAC AAGACCAACA ATCCATTATA 
CTTGTAAATG ATTCAGCAAT CCTAGAAGTA CGCAAGGAAA GTCACCCCTT GGCTTTCGAG 
TGTGACACAC TGGATAATAA TGAAATAGTA GCAACTATTA AATTCACAGT CTATACGAGC 
AGTGAATTGC AGATGAGAAG ATCAAGCCTA CCAGCCACTG ATGCAGCCCT AATTTTTGTG 
CTGACCATAG GAGTCATTAT CTGTGTATTT ATAATTTTCT TATTGATCTT CATAATCATA 
AATTGGGCAG CAGTCAAGGC TTTCTGGGGG GCAAAAGCCT CTACACCTGA GGTACAATCC 
GAGCAGAGTT CTGTGAGATA CAAAGATTCA ACTTCTCTTG ACCAATTACC AACAGAAATG 
CCTGGTGAAG ATGATGCTTT AAGTGAATGG AATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866824.1
CDS	314..952
Translation	MCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSLDQLPTEMPGEDDALSEWNE

Target ORF information:

RefSeq Version	XM_017011335.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm acrosome associated 1 (SPACA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011335.1

ORF Insert Sequence:

ATGTGCACCG TTACATGTGG TATTGGTGTT AGAGAAGTTA TATTAACAAA TGGATGCCCT 
GGTGGTGAAT CCAAGTGTGT TGTACGGGTA GAAGAATGCC GTGGACCAAC AGATTGTGGC 
TGGGGTAAAC CAATTTCAGA AAGTCTTGAA AGTGTTAGAT TGGCATGTAT TCACACATCT 
CCCTTAAATC GTTTCAAATA TATGTGGAAA CTTCTAAGAC AAGACCAACA ATCCATTATA 
CTTGTAAATG ATTCAGCAAT CCTAGAAGTA CGCAAGGAAA GTCACCCCTT GGCTTTCGAG 
TGTGACACAC TGGATAATAA TGAAATAGTA GCAACTATTA AATTCACAGT CTATACGAGC 
AGTGAATTGC AGATGAGAAG ATCAAGCCTA CCAGCCACTG ATGCAGCCCT AATTTTTGTG 
CTGACCATAG GAGTCATTAT CTGTGTATTT ATAATTTTCT TATTGATCTT CATAATCATA 
AATTGGGCAG CAGTCAAGGC TTTCTGGGGG GCAAAAGCCT CTACACCTGA GGTACAATCC 
GAGCAGAGTT CTGTGAGATA CAAAGATTCA ACTTCTCTTG ACCAATTACC AACAGAAATG 
CCTGGTGAAG ATGATGCTTT AAGTGAATGG AATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization.
Zygote (Cambridge, England)24(5)654-61(2016 Oct)
Kishida K,Harayama H,Kimura F,Murakami T

Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.
Reproduction, fertility, and development28(4)416-27(2016 Mar)
Chen X,Liu X,Ren X,Li X,Wang L,Zang W

L-type voltage-dependent calcium channel alpha subunit 1C is a novel candidate gene associated with secondary hyperparathyroidism: an application of haplotype-based analysis for multiple linked single nucleotide polymorphisms.
Nephron. Clinical practice115(4)c237-43(2010)
Yokoyama K,Urashima M,Ohkido I,Kono T,Yoshida T,Muramatsu M,Niu T,Hosoya T

Genome-wide association of echocardiographic dimensions, brachial artery endothelial function and treadmill exercise responses in the Framingham Heart Study.
BMC medical genetics8 Suppl 1S2(2007 Sep)
Vasan RS,Larson MG,Aragam J,Wang TJ,Mitchell GF,Kathiresan S,Newton-Cheh C,Vita JA,Keyes MJ,O'Donnell CJ,Levy D,Benjamin EJ

The DNA sequence and analysis of human chromosome 6.
Nature425(6960)805-11(2003 Oct)
Mungall AJ,Palmer SA,Sims SK,Edwards CA,Ashurst JL,Wilming L,Jones MC,Horton R,Hunt SE,Scott CE,Gilbert JG,Clamp ME,Bethel G,Milne S,Ainscough R,Almeida JP,Ambrose KD,Andrews TD,Ashwell RI,Babbage AK,Bagguley CL,Bailey J,Banerjee R,Barker DJ,Barlow KF,Bates K,Beare DM,Beasley H,Beasley O,Bird CP,Blakey S,Bray-Allen S,Brook J,Brown AJ,Brown JY,Burford DC,Burrill W,Burton J,Carder C,Carter NP,Chapman JC,Clark SY,Clark G,Clee CM,Clegg S,Cobley V,Collier RE,Collins JE,Colman LK,Corby NR,Coville GJ,Culley KM,Dhami P,Davies J,Dunn M,Earthrowl ME,Ellington AE,Evans KA,Faulkner L,Francis MD,Frankish A,Frankland J,French L,Garner P,Garnett J,Ghori MJ,Gilby LM,Gillson CJ,Glithero RJ,Grafham DV,Grant M,Gribble S,Griffiths C,Griffiths M,Hall R,Halls KS,Hammond S,Harley JL,Hart EA,Heath PD,Heathcott R,Holmes SJ,Howden PJ,Howe KL,Howell GR,Huckle E,Humphray SJ,Humphries MD,Hunt AR,Johnson CM,Joy AA,Kay M,Keenan SJ,Kimberley AM,King A,Laird GK,Langford C,Lawlor S,Leongamornlert DA,Leversha M,Lloyd CR,Lloyd DM,Loveland JE,Lovell J,Martin S,Mashreghi-Mohammadi M,Maslen GL,Matthews L,McCann OT,McLaren SJ,McLay K,McMurray A,Moore MJ,Mullikin JC,Niblett D,Nickerson T,Novik KL,Oliver K,Overton-Larty EK,Parker A,Patel R,Pearce AV,Peck AI,Phillimore B,Phillips S,Plumb RW,Porter KM,Ramsey Y,Ranby SA,Rice CM,Ross MT,Searle SM,Sehra HK,Sheridan E,Skuce CD,Smith S,Smith M,Spraggon L,Squares SL,Steward CA,Sycamore N,Tamlyn-Hall G,Tester J,Theaker AJ,Thomas DW,Thorpe A,Tracey A,Tromans A,Tubby B,Wall M,Wallis JM,West AP,White SS,Whitehead SL,Whittaker H,Wild A,Willey DJ,Wilmer TE,Wood JM,Wray PW,Wyatt JC,Young L,Younger RM,Bentley DR,Coulson A,Durbin R,Hubbard T,Sulston JE,Dunham I,Rogers J,Beck S

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SPACA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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