MADCAM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADCAM1
Entrez Gene ID	8174
Full Name	mucosal vascular addressin cell adhesion molecule 1
Synonyms	MACAM1
General protein information	Preferred Names mucosal vascular addressin cell adhesion molecule 1 Names mucosal addressin cell adhesion molecule 1 MAdCAM-1 hMAdCAM-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	102670

mRNA and Protein(s)

mRNA	Protein	Name
NM_130760.2	NP_570116.2	mucosal addressin cell adhesion molecule 1 isoform a precursor
NM_130762.2	NP_570118.1	mucosal addressin cell adhesion molecule 1 isoform b precursor
NM_130762.3	NP_570118.1	mucosal addressin cell adhesion molecule 1 isoform b precursor
NM_130760.3	NP_570116.2	mucosal addressin cell adhesion molecule 1 isoform a precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Development and validation of receptor occupancy pharmacodynamic assays used in the clinical development of the monoclonal antibody vedolizumab.
Wyant T, Estevam J, Yang L, Rosario M
Cytometry. Part B, Clinical cytometry90(2)168-76(2016 Mar)

Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells.
Wang J, Ma L, Tang X, Zhang X, Qiao Y, Shi Y, Xu Y, Wang Z, Yu Y, Sun F
Oncotarget6(27)24075-91(2015 Sep)

Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of ????.
Zhang K, Pan Y, Qi J, Yue J, Zhang M, Xu C, Li G, Chen J
Journal of cell science126(Pt 21)5030-41(2013 Nov)

Mucosal addressin cell adhesion molecule (MAdCAM-1) expression is upregulated in the cirrhotic liver and immunolocalises to the peribiliary plexus and lymphoid aggregates.
Ala A, Brown D, Khan K, Standish R, Odin JA, Fiel MI, Schiano TD, Hillan KJ, Rahman SA, Hodgson HJ, Dhillon AP
Digestive diseases and sciences58(9)2528-41(2013 Sep)

Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop.
Yu Y, Zhu J, Huang PS, Wang JH, Pullen N, Springer TA
The Journal of biological chemistry288(9)6284-94(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested.
Title: Development and validation of receptor occupancy pharmacodynamic assays used in the clinical development of the monoclonal antibody vedolizumab.

Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation
Title: Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells.

MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates
Title: Mucosal addressin cell adhesion molecule (MAdCAM-1) expression is upregulated in the cirrhotic liver and immunolocalises to the peribiliary plexus and lymphoid aggregates.

Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop.
Title: Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop.

Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding.
Title: The CC' and DE loops in Ig domains 1 and 2 of MAdCAM-1 play different roles in MAdCAM-1 binding to low- and high-affinity integrin alpha4beta7.

The following MADCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13360	NM_130762.3 Latest version!	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu13301	NM_130760.3 Latest version!	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu13360	NM_130762.2	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu13301	NM_130760.2	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADCAM1 ( NM_130762.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_570118.1 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13360
Clone ID Related Accession (Same CDS sequence)	NM_130762.2 , NM_130762.3
Accession Version	NM_130762.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	mucosal addressin cell adhesion molecule 1 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82483.1 and AY732484.1. On Jun 23, 2006 this sequence version replaced NM_130762.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) that lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2152568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG 
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC 
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC 
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570118.1
CDS	11..898
Translation	MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPASKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAEDDTHPPASLRLLPQVSAWAGLRGTGQVGISPS

Target ORF information:

RefSeq Version	NM_130762.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130762.2

ORF Insert Sequence:

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG 
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC 
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC 
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADCAM1 ( NM_130762.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_570118.1 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13360
Clone ID Related Accession (Same CDS sequence)	NM_130762.2 , NM_130762.3
Accession Version	NM_130762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	mucosal addressin cell adhesion molecule 1 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005775.1, U82483.1 and AY732484.1. On May 31, 2019 this sequence version replaced NM_130762.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) that lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2152568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG 
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC 
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC 
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570118.1
CDS	15..902
Translation	MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPASKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAEDDTHPPASLRLLPQVSAWAGLRGTGQVGISPS

Target ORF information:

RefSeq Version	NM_130762.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130762.3

ORF Insert Sequence:

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG 
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC 
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC 
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADCAM1 ( NM_130760.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_570116.2 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13301
Clone ID Related Accession (Same CDS sequence)	NM_130760.2 , NM_130760.3
Accession Version	NM_130760.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	mucosal addressin cell adhesion molecule 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82483.1, U43628.1 and DB323303.1. On or before Jun 23, 2006 this sequence version replaced NM_130761.1, NM_130760.1. Transcript Variant: This variant (1) is the longer, predominant transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U82483.1, AY819760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT 
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC 
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC 
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC 
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG 
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC 
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG 
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC 
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570116.2
CDS	11..1159
Translation	MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAEDDTHPPASLRLLPQVSAWAGLRGTGQVGISPS

Target ORF information:

RefSeq Version	NM_130760.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130760.2

ORF Insert Sequence:

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT 
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC 
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC 
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC 
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG 
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC 
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG 
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC 
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADCAM1 ( NM_130760.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_570116.2 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13301
Clone ID Related Accession (Same CDS sequence)	NM_130760.2 , NM_130760.3
Accession Version	NM_130760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	mucosal addressin cell adhesion molecule 1 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005775.1, U82483.1, U43628.1 and DB323303.1. On Nov 22, 2018 this sequence version replaced NM_130760.2. Transcript Variant: This variant (1) is the longer, predominant transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U82483.1, AY819760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000215637.8/ ENSP00000215637.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT 
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC 
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC 
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC 
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG 
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC 
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG 
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC 
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570116.2
CDS	15..1163
Translation	MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAEDDTHPPASLRLLPQVSAWAGLRGTGQVGISPS

Target ORF information:

RefSeq Version	NM_130760.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130760.3

ORF Insert Sequence:

ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC 
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG 
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC 
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC 
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC 
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG 
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG 
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC 
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC 
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC 
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT 
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC 
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC 
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC 
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG 
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC 
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG 
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC 
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development and validation of receptor occupancy pharmacodynamic assays used in the clinical development of the monoclonal antibody vedolizumab.
Cytometry. Part B, Clinical cytometry90(2)168-76(2016 Mar)
Wyant T,Estevam J,Yang L,Rosario M

Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells.
Oncotarget6(27)24075-91(2015 Sep)
Wang J,Ma L,Tang X,Zhang X,Qiao Y,Shi Y,Xu Y,Wang Z,Yu Y,Sun F

Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of ????.
Journal of cell science126(Pt 21)5030-41(2013 Nov)
Zhang K,Pan Y,Qi J,Yue J,Zhang M,Xu C,Li G,Chen J

Mucosal addressin cell adhesion molecule (MAdCAM-1) expression is upregulated in the cirrhotic liver and immunolocalises to the peribiliary plexus and lymphoid aggregates.
Digestive diseases and sciences58(9)2528-41(2013 Sep)
Ala A,Brown D,Khan K,Standish R,Odin JA,Fiel MI,Schiano TD,Hillan KJ,Rahman SA,Hodgson HJ,Dhillon AP

Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop.
The Journal of biological chemistry288(9)6284-94(2013 Mar)
Yu Y,Zhu J,Huang PS,Wang JH,Pullen N,Springer TA

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MADCAM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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