TRMT1L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT1L
Entrez Gene ID	81627
Full Name	tRNA methyltransferase 1 like
Synonyms	C1orf25,MST070,MSTP070,TRM1L,bG120K12.3
General protein information	Preferred Names tRNA methyltransferase 1 like Names TRMT1-like protein TRM1 tRNA methyltransferase 1-like TRM1-like protein tRNA methyltransferase 1 homolog-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.3
Summary	This gene encodes a protein that has some similarity to N2,N2-dimethylguanosine tRNA methyltransferase from other organisms. Studies of the mouse ortholog have shown that this protein plays a role in motor coordination and exploratory behavior, and it may also be involved in modulating postnatal neuronal functions. Alternatively spliced transcripts have been identified for this gene. [provided by RefSeq, Jan 2011].
Disorder MIM:	611673

mRNA and Protein(s)

mRNA	Protein	Name
XM_011510028.2	XP_011508330.1	TRMT1-like protein isoform X1
NM_030934.5	NP_112196.3	TRMT1-like protein isoform 1
NM_030934.4	NP_112196.3	TRMT1-like protein isoform 1
NM_001202423.1	NP_001189352.1	TRMT1-like protein isoform 2
XM_017002419.1	XP_016857908.1	TRMT1-like protein isoform X2
XM_017002419.2	XP_016857908.1	TRMT1-like protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT1L	NP_112196.3
Canis lupus familiaris (dog)	TRMT1L	XP_849853.1
Rattus norvegicus (Norway rat)	Trmt1l	NP_001032269.1
Pan troglodytes (chimpanzee)	TRMT1L	XP_514058.2
	TRMT1L	XP_001113820.1
Xenopus tropicalis (tropical clawed frog)	trmt1l	XP_004913842.1
Gallus gallus (chicken)	TRMT1L	XP_003643222.2
Danio rerio (zebrafish)	trmt1l	XP_688031.4
Bos taurus (cattle)	TRMT1L	NP_001091590.1
Mus musculus (house mouse)	Trmt1l	NP_081152.2

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mouse Trm1-like gene is expressed in neural tissues and plays a role in motor coordination and exploratory behaviour.
Vauti F, Goller T, Beine R, Becker L, Klopstock T, H?lter SM, Wurst W, Fuchs H, Gailus-Durner V, de Angelis MH, Arnold HH
Gene389(2)174-85(2007 Mar)

Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus.
Sood R, Bonner TI, Makalowska I, Stephan DA, Robbins CM, Connors TD, Morgenbesser SD, Su K, Faruque MU, Pinkett H, Graham C, Baxevanis AD, Klinger KW, Landes GM, Trent JM, Carpten JD
Genomics73(2)211-22(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional characterization of the mouse C1orf25 ortholog and comparison to the human protein.
Title: The mouse Trm1-like gene is expressed in neural tissues and plays a role in motor coordination and exploratory behaviour.

Includes the identification of the C1orf25 gene.
Title: Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus.

The following TRMT1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04551	NM_030934.5 Latest version!	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu55660	NM_001202423.1 Latest version!	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu55660	XM_011510028.2 Latest version!	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu90577	XM_017002419.2 Latest version!	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu04551	NM_030934.4	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu90577	XM_017002419.1	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT1L ( NM_030934.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_112196.3 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.

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CloneID	OHu04551
Clone ID Related Accession (Same CDS sequence)	NM_030934.5 , NM_030934.4
Accession Version	NM_030934.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB093548.1, BC045535.1, AK226103.1 and AF288399.1. On Nov 22, 2018 this sequence version replaced NM_030934.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803611.205757.1, SRR1660807.101521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367506.10/ ENSP00000356476.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGAAAACCC ATATAGACAG CTGCCTTGTA ACTGTCATGG AAGCATGCCT 
GGAAAGACAG CAATAGAACT TGGACCTCTG TGGTCAAGTT CCCTTTTCAA TACTGGATTC 
CTCAAAAGAA TGCTATTTGA ATCTCTTCAC CATGGTTTGG ATGACATTCA GACCCTAATA 
AAGACATTAA TCTTTGAATC AGAGTGTACG CCTCAAAGTC AGTTTTCAAT TCATGCATCT 
TCAAATGTCA ACAAGCAAGA AGAAAATGGT GTATTTATTA AAACTACAGA TGACACCACA 
ACAGATAATT ACATTGCACA AGGAAAGAGA AAAAGTAATG AAATGATCAC AAATTTAGGC 
AAGAAGCAAA AGACTGATGT CAGTACTGAA CATCCTCCCT TTTATTACAA CATTCACAGA 
CACAGCATTA AAGGAATGAA TATGCCAAAG TTAAAAAAGT TTTTGTGCTA TTTATCTCAA 
GCAGGCTTTC GAGTAAGCCG AACTCATTTT GACCCAATGG GTGTACGCAC AGATGCACCT 
CTGATGCAGT TTAAATCTAT CCTTTTAAAG TACAGCACCC CCACCTACAC TGGAGGACAG 
TCAGAAAGCC ATGTCCAGTC AGCATCTGAA GATACAGTAA CTGAAAGAGT TGAAATGTCA 
GTGAATGACA AAGCAGAAGC AAGTGGCTGC AGAAGATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112196.3
CDS	242..2443
Translation	MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW

Target ORF information:

RefSeq Version	NM_030934.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030934.5

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGAAAACCC ATATAGACAG CTGCCTTGTA ACTGTCATGG AAGCATGCCT 
GGAAAGACAG CAATAGAACT TGGACCTCTG TGGTCAAGTT CCCTTTTCAA TACTGGATTC 
CTCAAAAGAA TGCTATTTGA ATCTCTTCAC CATGGTTTGG ATGACATTCA GACCCTAATA 
AAGACATTAA TCTTTGAATC AGAGTGTACG CCTCAAAGTC AGTTTTCAAT TCATGCATCT 
TCAAATGTCA ACAAGCAAGA AGAAAATGGT GTATTTATTA AAACTACAGA TGACACCACA 
ACAGATAATT ACATTGCACA AGGAAAGAGA AAAAGTAATG AAATGATCAC AAATTTAGGC 
AAGAAGCAAA AGACTGATGT CAGTACTGAA CATCCTCCCT TTTATTACAA CATTCACAGA 
CACAGCATTA AAGGAATGAA TATGCCAAAG TTAAAAAAGT TTTTGTGCTA TTTATCTCAA 
GCAGGCTTTC GAGTAAGCCG AACTCATTTT GACCCAATGG GTGTACGCAC AGATGCACCT 
CTGATGCAGT TTAAATCTAT CCTTTTAAAG TACAGCACCC CCACCTACAC TGGAGGACAG 
TCAGAAAGCC ATGTCCAGTC AGCATCTGAA GATACAGTAA CTGAAAGAGT TGAAATGTCA 
GTGAATGACA AAGCAGAAGC AAGTGGCTGC AGAAGATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( NM_001202423.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189352.1 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55660
Clone ID Related Accession (Same CDS sequence)	XM_011510028.2 , NM_001202423.1
Accession Version	NM_001202423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398320.1, AK302166.1, AK074993.1, AF163261.1, AK226103.1 and BU198525.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.271710.1, AK302166.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCATCT GTCACTTACC TTGTCGACCA GTGAAACCAA ACATTATTGG AGAACAGATA 
ACCAGTAAAA TGGGAGCCCA TTATCATTGT ATCATTTGTT CAGCAACAAT CACCAGAAGA 
ACTGATATGC TAGGACATGT TAGGCGCCAC ATGAATAAAG GAGAGACTAA ATCTAGTTAT 
ATTGCAGCTT CCACTGCTAA ACCACCTAAG GAAATTTTGA AAGAGGCAGA CACGGATGTA 
CAAGTTTGTC CCAACTATTC TATACCTCAG AAAACAGATT CCTATTTTAA CCCCAAAATG 
AAACTAAATC GGCAGCTAAT ATTCTGTACA TTGGCTGCTT TGGCTGAGGA ACGAAAACCT 
TTGGAATGTC TAGATGCTTT TGGAGCCACT GGGATAATGG GATTACAGTG GGCAAAACAT 
CTTGGAAATG CAGTCAAAGT TACAATCAAT GACTTGAATG AAAATTCTGT GACACTGATT 
CAGGAAAACT GCCATTTAAA CAAATTGAAA GTGGTGGTGG ACAGTAAGGA AAAGGAAAAG 
AGTGATGATA TTCTTGAAGA AGGAGAGAAA AATCTTGGTA ATATTAAGGT GACCAAAATG 
GATGCCAATG TACTGATGCA TTTGAGATCT TTTGATTTCA TACATCTAGA CCCTTTTGGA 
ACATCAGTGA ATTATCTAGA TTCTGCATTC AGAAATATAA GAAACCTTGG CATAGTGTCA 
GTGACTTCTA CAGATATCAG TTCTTTATAT GCCAAGGCAC AGCATGTTGC CCGGCGTCAC 
TACGGATGTA ACATTGTCCG AACTGAATAT TACAAGGAAC TAGCAGCCAG AATTGTTGTA 
GCTGCAGTGG CAAGAGCTGC AGCCCGATGC AACAAAGGCA TAGAAGTACT GTTTGCAGTG 
GCTCTGGAAC ATTTTGTGTT GGTAGTTGTG AGAGTTTTGA GGGGACCTAC TTCAGCAGAT 
GAAACAGCCA AGAAGATTCA ATACCTGATC CATTGTCAGT GGTGTGAAGA GAGAATTTTT 
CAGAAGGATG GTAATATGGT AGAAGAAAAC CCATATAGAC AGCTGCCTTG TAACTGTCAT 
GGAAGCATGC CTGGAAAGAC AGCAATAGAA CTTGGACCTC TGTGGTCAAG TTCCCTTTTC 
AATACTGGAT TCCTCAAAAG AATGCTATTT GAATCTCTTC ACCATGGTTT GGATGACATT 
CAGACCCTAA TAAAGACATT AATCTTTGAA TCAGAGTGTA CGCCTCAAAG TCAGTTTTCA 
ATTCATGCAT CTTCAAATGT CAACAAGCAA GAAGAAAATG GTGTATTTAT TAAAACTACA 
GATGACACCA CAACAGATAA TTACATTGCA CAAGGAAAGA GAAAAAGTAA TGAAATGATC 
ACAAATTTAG GCAAGAAGCA AAAGACTGAT GTCAGTACTG AACATCCTCC CTTTTATTAC 
AACATTCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATTTATCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGTGTACGC 
ACAGATGCAC CTCTGATGCA GTTTAAATCT ATCCTTTTAA AGTACAGCAC CCCCACCTAC 
ACTGGAGGAC AGTCAGAAAG CCATGTCCAG TCAGCATCTG AAGATACAGT AACTGAAAGA 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAAGTGGCT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189352.1
CDS	349..2082
Translation	MCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW

Target ORF information:

RefSeq Version	NM_001202423.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202423.1

ORF Insert Sequence:

ATGTGCATCT GTCACTTACC TTGTCGACCA GTGAAACCAA ACATTATTGG AGAACAGATA 
ACCAGTAAAA TGGGAGCCCA TTATCATTGT ATCATTTGTT CAGCAACAAT CACCAGAAGA 
ACTGATATGC TAGGACATGT TAGGCGCCAC ATGAATAAAG GAGAGACTAA ATCTAGTTAT 
ATTGCAGCTT CCACTGCTAA ACCACCTAAG GAAATTTTGA AAGAGGCAGA CACGGATGTA 
CAAGTTTGTC CCAACTATTC TATACCTCAG AAAACAGATT CCTATTTTAA CCCCAAAATG 
AAACTAAATC GGCAGCTAAT ATTCTGTACA TTGGCTGCTT TGGCTGAGGA ACGAAAACCT 
TTGGAATGTC TAGATGCTTT TGGAGCCACT GGGATAATGG GATTACAGTG GGCAAAACAT 
CTTGGAAATG CAGTCAAAGT TACAATCAAT GACTTGAATG AAAATTCTGT GACACTGATT 
CAGGAAAACT GCCATTTAAA CAAATTGAAA GTGGTGGTGG ACAGTAAGGA AAAGGAAAAG 
AGTGATGATA TTCTTGAAGA AGGAGAGAAA AATCTTGGTA ATATTAAGGT GACCAAAATG 
GATGCCAATG TACTGATGCA TTTGAGATCT TTTGATTTCA TACATCTAGA CCCTTTTGGA 
ACATCAGTGA ATTATCTAGA TTCTGCATTC AGAAATATAA GAAACCTTGG CATAGTGTCA 
GTGACTTCTA CAGATATCAG TTCTTTATAT GCCAAGGCAC AGCATGTTGC CCGGCGTCAC 
TACGGATGTA ACATTGTCCG AACTGAATAT TACAAGGAAC TAGCAGCCAG AATTGTTGTA 
GCTGCAGTGG CAAGAGCTGC AGCCCGATGC AACAAAGGCA TAGAAGTACT GTTTGCAGTG 
GCTCTGGAAC ATTTTGTGTT GGTAGTTGTG AGAGTTTTGA GGGGACCTAC TTCAGCAGAT 
GAAACAGCCA AGAAGATTCA ATACCTGATC CATTGTCAGT GGTGTGAAGA GAGAATTTTT 
CAGAAGGATG GTAATATGGT AGAAGAAAAC CCATATAGAC AGCTGCCTTG TAACTGTCAT 
GGAAGCATGC CTGGAAAGAC AGCAATAGAA CTTGGACCTC TGTGGTCAAG TTCCCTTTTC 
AATACTGGAT TCCTCAAAAG AATGCTATTT GAATCTCTTC ACCATGGTTT GGATGACATT 
CAGACCCTAA TAAAGACATT AATCTTTGAA TCAGAGTGTA CGCCTCAAAG TCAGTTTTCA 
ATTCATGCAT CTTCAAATGT CAACAAGCAA GAAGAAAATG GTGTATTTAT TAAAACTACA 
GATGACACCA CAACAGATAA TTACATTGCA CAAGGAAAGA GAAAAAGTAA TGAAATGATC 
ACAAATTTAG GCAAGAAGCA AAAGACTGAT GTCAGTACTG AACATCCTCC CTTTTATTAC 
AACATTCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATTTATCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGTGTACGC 
ACAGATGCAC CTCTGATGCA GTTTAAATCT ATCCTTTTAA AGTACAGCAC CCCCACCTAC 
ACTGGAGGAC AGTCAGAAAG CCATGTCCAG TCAGCATCTG AAGATACAGT AACTGAAAGA 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAAGTGGCT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_011510028.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508330.1 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

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CloneID	OHu55660
Clone ID Related Accession (Same CDS sequence)	XM_011510028.2 , NM_001202423.1
Accession Version	XM_011510028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATCT GTCACTTACC TTGTCGACCA GTGAAACCAA ACATTATTGG AGAACAGATA 
ACCAGTAAAA TGGGAGCCCA TTATCATTGT ATCATTTGTT CAGCAACAAT CACCAGAAGA 
ACTGATATGC TAGGACATGT TAGGCGCCAC ATGAATAAAG GAGAGACTAA ATCTAGTTAT 
ATTGCAGCTT CCACTGCTAA ACCACCTAAG GAAATTTTGA AAGAGGCAGA CACGGATGTA 
CAAGTTTGTC CCAACTATTC TATACCTCAG AAAACAGATT CCTATTTTAA CCCCAAAATG 
AAACTAAATC GGCAGCTAAT ATTCTGTACA TTGGCTGCTT TGGCTGAGGA ACGAAAACCT 
TTGGAATGTC TAGATGCTTT TGGAGCCACT GGGATAATGG GATTACAGTG GGCAAAACAT 
CTTGGAAATG CAGTCAAAGT TACAATCAAT GACTTGAATG AAAATTCTGT GACACTGATT 
CAGGAAAACT GCCATTTAAA CAAATTGAAA GTGGTGGTGG ACAGTAAGGA AAAGGAAAAG 
AGTGATGATA TTCTTGAAGA AGGAGAGAAA AATCTTGGTA ATATTAAGGT GACCAAAATG 
GATGCCAATG TACTGATGCA TTTGAGATCT TTTGATTTCA TACATCTAGA CCCTTTTGGA 
ACATCAGTGA ATTATCTAGA TTCTGCATTC AGAAATATAA GAAACCTTGG CATAGTGTCA 
GTGACTTCTA CAGATATCAG TTCTTTATAT GCCAAGGCAC AGCATGTTGC CCGGCGTCAC 
TACGGATGTA ACATTGTCCG AACTGAATAT TACAAGGAAC TAGCAGCCAG AATTGTTGTA 
GCTGCAGTGG CAAGAGCTGC AGCCCGATGC AACAAAGGCA TAGAAGTACT GTTTGCAGTG 
GCTCTGGAAC ATTTTGTGTT GGTAGTTGTG AGAGTTTTGA GGGGACCTAC TTCAGCAGAT 
GAAACAGCCA AGAAGATTCA ATACCTGATC CATTGTCAGT GGTGTGAAGA GAGAATTTTT 
CAGAAGGATG GTAATATGGT AGAAGAAAAC CCATATAGAC AGCTGCCTTG TAACTGTCAT 
GGAAGCATGC CTGGAAAGAC AGCAATAGAA CTTGGACCTC TGTGGTCAAG TTCCCTTTTC 
AATACTGGAT TCCTCAAAAG AATGCTATTT GAATCTCTTC ACCATGGTTT GGATGACATT 
CAGACCCTAA TAAAGACATT AATCTTTGAA TCAGAGTGTA CGCCTCAAAG TCAGTTTTCA 
ATTCATGCAT CTTCAAATGT CAACAAGCAA GAAGAAAATG GTGTATTTAT TAAAACTACA 
GATGACACCA CAACAGATAA TTACATTGCA CAAGGAAAGA GAAAAAGTAA TGAAATGATC 
ACAAATTTAG GCAAGAAGCA AAAGACTGAT GTCAGTACTG AACATCCTCC CTTTTATTAC 
AACATTCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATTTATCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGTGTACGC 
ACAGATGCAC CTCTGATGCA GTTTAAATCT ATCCTTTTAA AGTACAGCAC CCCCACCTAC 
ACTGGAGGAC AGTCAGAAAG CCATGTCCAG TCAGCATCTG AAGATACAGT AACTGAAAGA 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAAGTGGCT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508330.1
CDS	2178..3911
Translation	MCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW

Target ORF information:

RefSeq Version	XM_011510028.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510028.2

ORF Insert Sequence:

ATGTGCATCT GTCACTTACC TTGTCGACCA GTGAAACCAA ACATTATTGG AGAACAGATA 
ACCAGTAAAA TGGGAGCCCA TTATCATTGT ATCATTTGTT CAGCAACAAT CACCAGAAGA 
ACTGATATGC TAGGACATGT TAGGCGCCAC ATGAATAAAG GAGAGACTAA ATCTAGTTAT 
ATTGCAGCTT CCACTGCTAA ACCACCTAAG GAAATTTTGA AAGAGGCAGA CACGGATGTA 
CAAGTTTGTC CCAACTATTC TATACCTCAG AAAACAGATT CCTATTTTAA CCCCAAAATG 
AAACTAAATC GGCAGCTAAT ATTCTGTACA TTGGCTGCTT TGGCTGAGGA ACGAAAACCT 
TTGGAATGTC TAGATGCTTT TGGAGCCACT GGGATAATGG GATTACAGTG GGCAAAACAT 
CTTGGAAATG CAGTCAAAGT TACAATCAAT GACTTGAATG AAAATTCTGT GACACTGATT 
CAGGAAAACT GCCATTTAAA CAAATTGAAA GTGGTGGTGG ACAGTAAGGA AAAGGAAAAG 
AGTGATGATA TTCTTGAAGA AGGAGAGAAA AATCTTGGTA ATATTAAGGT GACCAAAATG 
GATGCCAATG TACTGATGCA TTTGAGATCT TTTGATTTCA TACATCTAGA CCCTTTTGGA 
ACATCAGTGA ATTATCTAGA TTCTGCATTC AGAAATATAA GAAACCTTGG CATAGTGTCA 
GTGACTTCTA CAGATATCAG TTCTTTATAT GCCAAGGCAC AGCATGTTGC CCGGCGTCAC 
TACGGATGTA ACATTGTCCG AACTGAATAT TACAAGGAAC TAGCAGCCAG AATTGTTGTA 
GCTGCAGTGG CAAGAGCTGC AGCCCGATGC AACAAAGGCA TAGAAGTACT GTTTGCAGTG 
GCTCTGGAAC ATTTTGTGTT GGTAGTTGTG AGAGTTTTGA GGGGACCTAC TTCAGCAGAT 
GAAACAGCCA AGAAGATTCA ATACCTGATC CATTGTCAGT GGTGTGAAGA GAGAATTTTT 
CAGAAGGATG GTAATATGGT AGAAGAAAAC CCATATAGAC AGCTGCCTTG TAACTGTCAT 
GGAAGCATGC CTGGAAAGAC AGCAATAGAA CTTGGACCTC TGTGGTCAAG TTCCCTTTTC 
AATACTGGAT TCCTCAAAAG AATGCTATTT GAATCTCTTC ACCATGGTTT GGATGACATT 
CAGACCCTAA TAAAGACATT AATCTTTGAA TCAGAGTGTA CGCCTCAAAG TCAGTTTTCA 
ATTCATGCAT CTTCAAATGT CAACAAGCAA GAAGAAAATG GTGTATTTAT TAAAACTACA 
GATGACACCA CAACAGATAA TTACATTGCA CAAGGAAAGA GAAAAAGTAA TGAAATGATC 
ACAAATTTAG GCAAGAAGCA AAAGACTGAT GTCAGTACTG AACATCCTCC CTTTTATTAC 
AACATTCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATTTATCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGTGTACGC 
ACAGATGCAC CTCTGATGCA GTTTAAATCT ATCCTTTTAA AGTACAGCAC CCCCACCTAC 
ACTGGAGGAC AGTCAGAAAG CCATGTCCAG TCAGCATCTG AAGATACAGT AACTGAAAGA 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAAGTGGCT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( NM_030934.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_112196.3 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.

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CloneID	OHu04551
Clone ID Related Accession (Same CDS sequence)	NM_030934.5 , NM_030934.4
Accession Version	NM_030934.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB093548.1, BC045535.1, AK226103.1 and AF288399.1. On Dec 20, 2007 this sequence version replaced NM_030934.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK226103.1, SRR1660807.101521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGAAAACCC ATATAGACAG CTGCCTTGTA ACTGTCATGG AAGCATGCCT 
GGAAAGACAG CAATAGAACT TGGACCTCTG TGGTCAAGTT CCCTTTTCAA TACTGGATTC 
CTCAAAAGAA TGCTATTTGA ATCTCTTCAC CATGGTTTGG ATGACATTCA GACCCTAATA 
AAGACATTAA TCTTTGAATC AGAGTGTACG CCTCAAAGTC AGTTTTCAAT TCATGCATCT 
TCAAATGTCA ACAAGCAAGA AGAAAATGGT GTATTTATTA AAACTACAGA TGACACCACA 
ACAGATAATT ACATTGCACA AGGAAAGAGA AAAAGTAATG AAATGATCAC AAATTTAGGC 
AAGAAGCAAA AGACTGATGT CAGTACTGAA CATCCTCCCT TTTATTACAA CATTCACAGA 
CACAGCATTA AAGGAATGAA TATGCCAAAG TTAAAAAAGT TTTTGTGCTA TTTATCTCAA 
GCAGGCTTTC GAGTAAGCCG AACTCATTTT GACCCAATGG GTGTACGCAC AGATGCACCT 
CTGATGCAGT TTAAATCTAT CCTTTTAAAG TACAGCACCC CCACCTACAC TGGAGGACAG 
TCAGAAAGCC ATGTCCAGTC AGCATCTGAA GATACAGTAA CTGAAAGAGT TGAAATGTCA 
GTGAATGACA AAGCAGAAGC AAGTGGCTGC AGAAGATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112196.3
CDS	273..2474
Translation	MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW

Target ORF information:

RefSeq Version	NM_030934.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030934.4

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGAAAACCC ATATAGACAG CTGCCTTGTA ACTGTCATGG AAGCATGCCT 
GGAAAGACAG CAATAGAACT TGGACCTCTG TGGTCAAGTT CCCTTTTCAA TACTGGATTC 
CTCAAAAGAA TGCTATTTGA ATCTCTTCAC CATGGTTTGG ATGACATTCA GACCCTAATA 
AAGACATTAA TCTTTGAATC AGAGTGTACG CCTCAAAGTC AGTTTTCAAT TCATGCATCT 
TCAAATGTCA ACAAGCAAGA AGAAAATGGT GTATTTATTA AAACTACAGA TGACACCACA 
ACAGATAATT ACATTGCACA AGGAAAGAGA AAAAGTAATG AAATGATCAC AAATTTAGGC 
AAGAAGCAAA AGACTGATGT CAGTACTGAA CATCCTCCCT TTTATTACAA CATTCACAGA 
CACAGCATTA AAGGAATGAA TATGCCAAAG TTAAAAAAGT TTTTGTGCTA TTTATCTCAA 
GCAGGCTTTC GAGTAAGCCG AACTCATTTT GACCCAATGG GTGTACGCAC AGATGCACCT 
CTGATGCAGT TTAAATCTAT CCTTTTAAAG TACAGCACCC CCACCTACAC TGGAGGACAG 
TCAGAAAGCC ATGTCCAGTC AGCATCTGAA GATACAGTAA CTGAAAGAGT TGAAATGTCA 
GTGAATGACA AAGCAGAAGC AAGTGGCTGC AGAAGATGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_017002419.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857908.1 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.

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CloneID	OHu90577
Clone ID Related Accession (Same CDS sequence)	XM_017002419.1 , XM_017002419.2
Accession Version	XM_017002419.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGATTACAG TGCTAACTTT GTTATTTCCT ATACTGGATT TCCTTTCGTC 
AACAGACAAG ACATTCGTGT GAGTTTATTA AAGTTATTTA CTCATGCTGT ACAATATATG 
CTTTCTAGCT CTGCCTTCTA TCGAAACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857908.1
CDS	245..1897
Translation	MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEDYSANFVISYTGFPFVNRQDIRVSLLKLFTHAVQYMLSSSAFYRNI

Target ORF information:

RefSeq Version	XM_017002419.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002419.1

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGATTACAG TGCTAACTTT GTTATTTCCT ATACTGGATT TCCTTTCGTC 
AACAGACAAG ACATTCGTGT GAGTTTATTA AAGTTATTTA CTCATGCTGT ACAATATATG 
CTTTCTAGCT CTGCCTTCTA TCGAAACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_017002419.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857908.1 Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

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CloneID	OHu90577
Clone ID Related Accession (Same CDS sequence)	XM_017002419.1 , XM_017002419.2
Accession Version	XM_017002419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	TRMT1-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGATTACAG TGCTAACTTT GTTATTTCCT ATACTGGATT TCCTTTCGTC 
AACAGACAAG ACATTCGTGT GAGTTTATTA AAGTTATTTA CTCATGCTGT ACAATATATG 
CTTTCTAGCT CTGCCTTCTA TCGAAACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857908.1
CDS	253..1905
Translation	MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEDYSANFVISYTGFPFVNRQDIRVSLLKLFTHAVQYMLSSSAFYRNI

Target ORF information:

RefSeq Version	XM_017002419.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002419.2

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCTGGAGA AGGAGGAGGT GGAGGTGGCC 
CAGGTCCAGG TCCCGACCCC GGCCCGGGAC TCGGCTGGGG TCCCAGCTCC GGCCCCGGAT 
TCGGCTCTGG ACTCGGCTCC GACTCCGGCC TCGGCTCCAG CCCCAGCCCC TGCCCTGGCC 
CAGGCTCCGG CCCTGTCCCC GTCCCTAGCC TCTGCCCCTG AGGAGGCTAA AAGCAAGAGA 
CACATCTCAA TTCAAAGGCA GCTTGCTGAT CTAGAGAATT TAGCTTTTGT AACTGATGGA 
AATTTTGACT CTGCCAGCTC ATTGAACTCA GATAATCTTG ATGCAGGCAA CAGACAGGCT 
TGTCCATTGT GCCCTAAGGA AAAATTCAGA GCTTGTAATA GCCATAAGCT TCGTCGTCAC 
CTCCAGAATT TACACTGGAA AGTCTCAGTT GAATTTGAAG GTTACAGGAT GTGCATCTGT 
CACTTACCTT GTCGACCAGT GAAACCAAAC ATTATTGGAG AACAGATAAC CAGTAAAATG 
GGAGCCCATT ATCATTGTAT CATTTGTTCA GCAACAATCA CCAGAAGAAC TGATATGCTA 
GGACATGTTA GGCGCCACAT GAATAAAGGA GAGACTAAAT CTAGTTATAT TGCAGCTTCC 
ACTGCTAAAC CACCTAAGGA AATTTTGAAA GAGGCAGACA CGGATGTACA AGTTTGTCCC 
AACTATTCTA TACCTCAGAA AACAGATTCC TATTTTAACC CCAAAATGAA ACTAAATCGG 
CAGCTAATAT TCTGTACATT GGCTGCTTTG GCTGAGGAAC GAAAACCTTT GGAATGTCTA 
GATGCTTTTG GAGCCACTGG GATAATGGGA TTACAGTGGG CAAAACATCT TGGAAATGCA 
GTCAAAGTTA CAATCAATGA CTTGAATGAA AATTCTGTGA CACTGATTCA GGAAAACTGC 
CATTTAAACA AATTGAAAGT GGTGGTGGAC AGTAAGGAAA AGGAAAAGAG TGATGATATT 
CTTGAAGAAG GAGAGAAAAA TCTTGGTAAT ATTAAGGTGA CCAAAATGGA TGCCAATGTA 
CTGATGCATT TGAGATCTTT TGATTTCATA CATCTAGACC CTTTTGGAAC ATCAGTGAAT 
TATCTAGATT CTGCATTCAG AAATATAAGA AACCTTGGCA TAGTGTCAGT GACTTCTACA 
GATATCAGTT CTTTATATGC CAAGGCACAG CATGTTGCCC GGCGTCACTA CGGATGTAAC 
ATTGTCCGAA CTGAATATTA CAAGGAACTA GCAGCCAGAA TTGTTGTAGC TGCAGTGGCA 
AGAGCTGCAG CCCGATGCAA CAAAGGCATA GAAGTACTGT TTGCAGTGGC TCTGGAACAT 
TTTGTGTTGG TAGTTGTGAG AGTTTTGAGG GGACCTACTT CAGCAGATGA AACAGCCAAG 
AAGATTCAAT ACCTGATCCA TTGTCAGTGG TGTGAAGAGA GAATTTTTCA GAAGGATGGT 
AATATGGTAG AAGATTACAG TGCTAACTTT GTTATTTCCT ATACTGGATT TCCTTTCGTC 
AACAGACAAG ACATTCGTGT GAGTTTATTA AAGTTATTTA CTCATGCTGT ACAATATATG 
CTTTCTAGCT CTGCCTTCTA TCGAAACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mouse Trm1-like gene is expressed in neural tissues and plays a role in motor coordination and exploratory behaviour.
Gene389(2)174-85(2007 Mar)
Vauti F,Goller T,Beine R,Becker L,Klopstock T,H?lter SM,Wurst W,Fuchs H,Gailus-Durner V,de Angelis MH,Arnold HH

Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus.
Genomics73(2)211-22(2001 Apr)
Sood R,Bonner TI,Makalowska I,Stephan DA,Robbins CM,Connors TD,Morgenbesser SD,Su K,Faruque MU,Pinkett H,Graham C,Baxevanis AD,Klinger KW,Landes GM,Trent JM,Carpten JD

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TRMT1L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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