TMX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMX1
Entrez Gene ID	81542
Full Name	thioredoxin related transmembrane protein 1
Synonyms	PDIA11,TMX,TXNDC,TXNDC1
General protein information	Preferred Names thioredoxin related transmembrane protein 1 Names thioredoxin-related transmembrane protein 1 protein disulfide isomerase family A, member 11 thioredoxin domain containing 1 thioredoxin domain-containing protein 1 transmembrane Trx-related protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q22.1
Summary	This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and one transmembrane domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The mature membrane-bound protein can both oxidize and reduce disulfide bonds and acts selectively on membrane-associated polypeptides. [provided by RefSeq, Jan 2017].
Disorder MIM:	610527

mRNA and Protein(s)

mRNA	Protein	Name
NM_030755.4	NP_110382.3	thioredoxin-related transmembrane protein 1 precursor
NM_030755.5	NP_110382.3	thioredoxin-related transmembrane protein 1 precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	dpy-11	NP_504655.1
Pan troglodytes (chimpanzee)	TMX1	XP_522851.2
Canis lupus familiaris (dog)	TMX1	XP_853432.2
Bos taurus (cattle)	TMX1	NP_001068853.1
Danio rerio (zebrafish)	tmx1	NP_001108605.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001613	XP_321485.5
Homo sapiens (human)	TMX1	NP_110382.3
Rattus norvegicus (Norway rat)	Tmx1	NP_001019971.1
Drosophila melanogaster (fruit fly)	CG5554	NP_611838.1
	TMX1	XP_001102891.1
Mus musculus (house mouse)	Tmx1	NP_082615.1
Xenopus tropicalis (tropical clawed frog)	tmx1	NP_001016906.1

Gene Ontology
Bibliography

Related articles in PubMed

TMX1 determines cancer cell metabolism as a thiol-based modulator of ER-mitochondria Ca2+ flux.
Raturi A, Guti?rrez T, Ortiz-Sandoval C, Ruangkittisakul A, Herrera-Cruz MS, Rockley JP, Gesson K, Ourdev D, Lou PH, Lucchinetti E, Tahbaz N, Zaugg M, Baksh S, Ballanyi K, Simmen T
The Journal of cell biology214(4)433-44(2016 Aug)

Division of labor among oxidoreductases: TMX1 preferentially acts on transmembrane polypeptides.
Pisoni GB, Ruddock LW, Bulleid N, Molinari M
Molecular biology of the cell26(19)3390-400(2015 Oct)

The human protein disulfide isomerase gene family.
Galligan JJ, Petersen DR
Human genomics66(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TMX1 increases mitochondrial ATP production and apoptosis progression.
Title: TMX1 determines cancer cell metabolism as a thiol-based modulator of ER-mitochondria Ca2+ flux.

Here we show that TMX1, one of the few transmembrane members of the family, forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins
Title: Division of labor among oxidoreductases: TMX1 preferentially acts on transmembrane polypeptides.

We conclude that TMX plays a major role in host defense under the type of inflammatory conditions associated with oxidative stress.
Title: The protective role of the transmembrane thioredoxin-related protein TMX in inflammatory liver injury.

the silencing of TMX in the prostatic cell line DU145 reduced the sensitivity of the cells to ricin intoxication further confirming a role for this enzyme in intracellular ricin activation.
Title: Reductive activation of type 2 ribosome-inactivating proteins is promoted by transmembrane thioredoxin-related protein.

TMX1 is enriched on the mitochondria-associated membrane. Targeting TMX to the MAM requires palmitoylation of two membrane-proximal cytosolic cysteines.
Title: Palmitoylated TMX and calnexin target to the mitochondria-associated membrane.

The following TMX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17207	NM_030755.5 Latest version!	Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu17207	NM_030755.4	Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMX1 ( NM_030755.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_110382.3 Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.

Price & Availability↑

CloneID	OHu17207
Clone ID Related Accession (Same CDS sequence)	NM_030755.4 , NM_030755.5
Accession Version	NM_030755.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	thioredoxin-related transmembrane protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA203669.1, AB048246.1, BC056874.1, AL591807.1 and BU754378.1. On Jul 5, 2007 this sequence version replaced NM_030755.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.138510.1, SRR1660805.46843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCCT CCGGGAGTCT TGCAGTTCCC CTGGCAGTCC TGGTGCTGTT GCTTTGGGGT 
GCTCCCTGGA CGCACGGGCG GCGGAGCAAC GTTCGCGTCA TCACGGACGA GAACTGGAGA 
GAACTGCTGG AAGGAGACTG GATGATAGAA TTTTATGCCC CGTGGTGCCC TGCTTGTCAA 
AATCTTCAAC CGGAATGGGA AAGTTTTGCT GAATGGGGAG AAGATCTTGA GGTTAATATT 
GCGAAAGTAG ATGTCACAGA GCAGCCAGGA CTGAGTGGAC GGTTTATCAT AACTGCTCTT 
CCTACTATTT ATCATTGTAA AGATGGTGAA TTTAGGCGCT ATCAGGGTCC AAGGACTAAG 
AAGGACTTCA TAAACTTTAT AAGTGATAAA GAGTGGAAGA GTATTGAGCC CGTTTCATCA 
TGGTTTGGTC CAGGTTCTGT TCTGATGAGT AGTATGTCAG CACTCTTTCA GCTATCTATG 
TGGATCAGGA CTTGCCATAA CTACTTTATT GAAGACCTTG GATTGCCAGT GTGGGGATCA 
TATACTGTTT TTGCTTTAGC AACTCTGTTT TCCGGACTGT TATTAGGACT CTGTATGATA 
TTTGTGGCAG ATTGCCTTTG TCCTTCAAAA AGGCGCAGAC CACAGCCGTA CCCATACCCT 
TCAAAAAAAT TATTATCAGA ATCTGCACAA CCTTTGAAAA AAGTGGAGGA GGAACAAGAG 
GCGGATGAAG AAGATGTTTC AGAAGAAGAA GCTGAAAGTA AAGAAGGAAC AAACAAAGAC 
TTTCCACAGA ATGCCATAAG ACAACGCTCT CTGGGTCCAT CATTGGCCAC AGATAAATCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110382.3
CDS	126..968
Translation	MAPSGSLAVPLAVLVLLLWGAPWTHGRRSNVRVITDENWRELLEGDWMIEFYAPWCPACQNLQPEWESFAEWGEDLEVNIAKVDVTEQPGLSGRFIITALPTIYHCKDGEFRRYQGPRTKKDFINFISDKEWKSIEPVSSWFGPGSVLMSSMSALFQLSMWIRTCHNYFIEDLGLPVWGSYTVFALATLFSGLLLGLCMIFVADCLCPSKRRRPQPYPYPSKKLLSESAQPLKKVEEEQEADEEDVSEEEAESKEGTNKDFPQNAIRQRSLGPSLATDKS

Target ORF information:

RefSeq Version	NM_030755.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030755.4

ORF Insert Sequence:

ATGGCGCCCT CCGGGAGTCT TGCAGTTCCC CTGGCAGTCC TGGTGCTGTT GCTTTGGGGT 
GCTCCCTGGA CGCACGGGCG GCGGAGCAAC GTTCGCGTCA TCACGGACGA GAACTGGAGA 
GAACTGCTGG AAGGAGACTG GATGATAGAA TTTTATGCCC CGTGGTGCCC TGCTTGTCAA 
AATCTTCAAC CGGAATGGGA AAGTTTTGCT GAATGGGGAG AAGATCTTGA GGTTAATATT 
GCGAAAGTAG ATGTCACAGA GCAGCCAGGA CTGAGTGGAC GGTTTATCAT AACTGCTCTT 
CCTACTATTT ATCATTGTAA AGATGGTGAA TTTAGGCGCT ATCAGGGTCC AAGGACTAAG 
AAGGACTTCA TAAACTTTAT AAGTGATAAA GAGTGGAAGA GTATTGAGCC CGTTTCATCA 
TGGTTTGGTC CAGGTTCTGT TCTGATGAGT AGTATGTCAG CACTCTTTCA GCTATCTATG 
TGGATCAGGA CTTGCCATAA CTACTTTATT GAAGACCTTG GATTGCCAGT GTGGGGATCA 
TATACTGTTT TTGCTTTAGC AACTCTGTTT TCCGGACTGT TATTAGGACT CTGTATGATA 
TTTGTGGCAG ATTGCCTTTG TCCTTCAAAA AGGCGCAGAC CACAGCCGTA CCCATACCCT 
TCAAAAAAAT TATTATCAGA ATCTGCACAA CCTTTGAAAA AAGTGGAGGA GGAACAAGAG 
GCGGATGAAG AAGATGTTTC AGAAGAAGAA GCTGAAAGTA AAGAAGGAAC AAACAAAGAC 
TTTCCACAGA ATGCCATAAG ACAACGCTCT CTGGGTCCAT CATTGGCCAC AGATAAATCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMX1 ( NM_030755.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_110382.3 Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.

Price & Availability↑

CloneID	OHu17207
Clone ID Related Accession (Same CDS sequence)	NM_030755.4 , NM_030755.5
Accession Version	NM_030755.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	thioredoxin-related transmembrane protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB048246.1, BC056874.1, AL591807.1 and BU754378.1. On Nov 22, 2018 this sequence version replaced NM_030755.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.138510.1, SRR1660805.46843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000457354.7/ ENSP00000393316.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCCCT CCGGGAGTCT TGCAGTTCCC CTGGCAGTCC TGGTGCTGTT GCTTTGGGGT 
GCTCCCTGGA CGCACGGGCG GCGGAGCAAC GTTCGCGTCA TCACGGACGA GAACTGGAGA 
GAACTGCTGG AAGGAGACTG GATGATAGAA TTTTATGCCC CGTGGTGCCC TGCTTGTCAA 
AATCTTCAAC CGGAATGGGA AAGTTTTGCT GAATGGGGAG AAGATCTTGA GGTTAATATT 
GCGAAAGTAG ATGTCACAGA GCAGCCAGGA CTGAGTGGAC GGTTTATCAT AACTGCTCTT 
CCTACTATTT ATCATTGTAA AGATGGTGAA TTTAGGCGCT ATCAGGGTCC AAGGACTAAG 
AAGGACTTCA TAAACTTTAT AAGTGATAAA GAGTGGAAGA GTATTGAGCC CGTTTCATCA 
TGGTTTGGTC CAGGTTCTGT TCTGATGAGT AGTATGTCAG CACTCTTTCA GCTATCTATG 
TGGATCAGGA CTTGCCATAA CTACTTTATT GAAGACCTTG GATTGCCAGT GTGGGGATCA 
TATACTGTTT TTGCTTTAGC AACTCTGTTT TCCGGACTGT TATTAGGACT CTGTATGATA 
TTTGTGGCAG ATTGCCTTTG TCCTTCAAAA AGGCGCAGAC CACAGCCGTA CCCATACCCT 
TCAAAAAAAT TATTATCAGA ATCTGCACAA CCTTTGAAAA AAGTGGAGGA GGAACAAGAG 
GCGGATGAAG AAGATGTTTC AGAAGAAGAA GCTGAAAGTA AAGAAGGAAC AAACAAAGAC 
TTTCCACAGA ATGCCATAAG ACAACGCTCT CTGGGTCCAT CATTGGCCAC AGATAAATCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110382.3
CDS	47..889
Translation	MAPSGSLAVPLAVLVLLLWGAPWTHGRRSNVRVITDENWRELLEGDWMIEFYAPWCPACQNLQPEWESFAEWGEDLEVNIAKVDVTEQPGLSGRFIITALPTIYHCKDGEFRRYQGPRTKKDFINFISDKEWKSIEPVSSWFGPGSVLMSSMSALFQLSMWIRTCHNYFIEDLGLPVWGSYTVFALATLFSGLLLGLCMIFVADCLCPSKRRRPQPYPYPSKKLLSESAQPLKKVEEEQEADEEDVSEEEAESKEGTNKDFPQNAIRQRSLGPSLATDKS

Target ORF information:

RefSeq Version	NM_030755.5
Organism	Homo sapiens(human)
Definition	Homo sapiens thioredoxin related transmembrane protein 1 (TMX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030755.5

ORF Insert Sequence:

ATGGCGCCCT CCGGGAGTCT TGCAGTTCCC CTGGCAGTCC TGGTGCTGTT GCTTTGGGGT 
GCTCCCTGGA CGCACGGGCG GCGGAGCAAC GTTCGCGTCA TCACGGACGA GAACTGGAGA 
GAACTGCTGG AAGGAGACTG GATGATAGAA TTTTATGCCC CGTGGTGCCC TGCTTGTCAA 
AATCTTCAAC CGGAATGGGA AAGTTTTGCT GAATGGGGAG AAGATCTTGA GGTTAATATT 
GCGAAAGTAG ATGTCACAGA GCAGCCAGGA CTGAGTGGAC GGTTTATCAT AACTGCTCTT 
CCTACTATTT ATCATTGTAA AGATGGTGAA TTTAGGCGCT ATCAGGGTCC AAGGACTAAG 
AAGGACTTCA TAAACTTTAT AAGTGATAAA GAGTGGAAGA GTATTGAGCC CGTTTCATCA 
TGGTTTGGTC CAGGTTCTGT TCTGATGAGT AGTATGTCAG CACTCTTTCA GCTATCTATG 
TGGATCAGGA CTTGCCATAA CTACTTTATT GAAGACCTTG GATTGCCAGT GTGGGGATCA 
TATACTGTTT TTGCTTTAGC AACTCTGTTT TCCGGACTGT TATTAGGACT CTGTATGATA 
TTTGTGGCAG ATTGCCTTTG TCCTTCAAAA AGGCGCAGAC CACAGCCGTA CCCATACCCT 
TCAAAAAAAT TATTATCAGA ATCTGCACAA CCTTTGAAAA AAGTGGAGGA GGAACAAGAG 
GCGGATGAAG AAGATGTTTC AGAAGAAGAA GCTGAAAGTA AAGAAGGAAC AAACAAAGAC 
TTTCCACAGA ATGCCATAAG ACAACGCTCT CTGGGTCCAT CATTGGCCAC AGATAAATCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TMX1 determines cancer cell metabolism as a thiol-based modulator of ER-mitochondria Ca2+ flux.
The Journal of cell biology214(4)433-44(2016 Aug)
Raturi A,Guti?rrez T,Ortiz-Sandoval C,Ruangkittisakul A,Herrera-Cruz MS,Rockley JP,Gesson K,Ourdev D,Lou PH,Lucchinetti E,Tahbaz N,Zaugg M,Baksh S,Ballanyi K,Simmen T

Division of labor among oxidoreductases: TMX1 preferentially acts on transmembrane polypeptides.
Molecular biology of the cell26(19)3390-400(2015 Oct)
Pisoni GB,Ruddock LW,Bulleid N,Molinari M

The human protein disulfide isomerase gene family.
Human genomics66(2012 Jul)
Galligan JJ,Petersen DR

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TMX1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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