NPL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPL
Entrez Gene ID	80896
Full Name	N-acetylneuraminate pyruvate lyase
Synonyms	C112,C1orf13,NAL,NPL1
General protein information	Preferred Names N-acetylneuraminate pyruvate lyase Names N-acetylneuraminate lyase N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) N-acetylneuraminic acid aldolase NALase dihydrodipicolinate synthetase homolog 1 sialate-pyruvate lyase sialic acid aldolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.3
Summary	This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011].
Disorder MIM:	611412

mRNA and Protein(s)

mRNA	Protein	Name
NM_001200051.2	NP_001186980.1	N-acetylneuraminate lyase isoform 4
NM_001200050.1	NP_001186979.1	N-acetylneuraminate lyase isoform 2
NM_030769.2	NP_110396.1	N-acetylneuraminate lyase isoform 1
NM_001200056.1	NP_001186985.1	N-acetylneuraminate lyase isoform 3
NM_001200051.1	NP_001186980.1	N-acetylneuraminate lyase isoform 4
NM_001200052.1	NP_001186981.1	N-acetylneuraminate lyase isoform 5
NM_001200056.2	NP_001186985.1	N-acetylneuraminate lyase isoform 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPL	NP_001039443.1
Mus musculus (house mouse)	Npl	NP_083025.1
Gallus gallus (chicken)	NPL	NP_001026731.1
Pan troglodytes (chimpanzee)	NPL	NP_001233421.1
Canis lupus familiaris (dog)	NPL	XP_005622466.1
Danio rerio (zebrafish)	LOC100535292	XP_005166992.1
Homo sapiens (human)	NPL	NP_110396.1
Rattus norvegicus (Norway rat)	Npl	NP_001014006.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007700	XP_308175.3
Danio rerio (zebrafish)	npl	NP_996934.2
Xenopus tropicalis (tropical clawed frog)	npl	NP_001011207.1

Gene Ontology
Bibliography

Related articles in PubMed

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
Bergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A
The Journal of biological chemistry287(34)28865-81(2012 Aug)

Homology modeling and molecular dynamics study on N-acetylneuraminate lyase.
Chu HY, Zheng QC, Zhao YS, Zhang HX
Journal of molecular modeling15(3)323-8(2009 Mar)

A novel splice variant of human gene NPL, mainly expressed in human liver, kidney and peripheral blood leukocyte.
Wu M, Gu S, Xu J, Zou X, Zheng H, Jin Z, Xie Y, Ji C, Mao Y
DNA sequence : the journal of DNA sequencing and mapping16(2)137-42(2005 Apr)

Characterization of a sialate pyruvate-lyase in the cytosol of human erythrocytes.
Bulai T, Bratosin D, Artenie V, Montreuil J
Biochimie84(7)655-60(2002 Jul)

The sialate-pyruvate lyase from pig kidney. Elucidation of the primary structure and expression of recombinant enzyme activity.
Traving C, Bruse P, W?chter A, Schauer R
European journal of biochemistry268(24)6473-86(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

3D structure model of N-acetylneuraminate lyase from human (hNAL, EC 4.1.3.3) was created and refined
Title: Homology modeling and molecular dynamics study on N-acetylneuraminate lyase.

an NPL splice variant is mainly expressed in human liver, kidney and peripheral blood leukocytes
Title: A novel splice variant of human gene NPL, mainly expressed in human liver, kidney and peripheral blood leukocyte.

The following NPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06180	NM_001200051.2 Latest version!	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu08401	NM_030769.2 Latest version!	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu07959	NM_001200056.2 Latest version!	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu08488	NM_001200050.1 Latest version!	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu08512	NM_001200052.1 Latest version!	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu06180	NM_001200051.1	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu07959	NM_001200056.1	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPL ( NM_001200051.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186980.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu06180
Clone ID Related Accession (Same CDS sequence)	NM_001200051.2 , NM_001200051.1
Accession Version	NM_001200051.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058003.1 and AI249145.1. On Jun 2, 2019 this sequence version replaced NM_001200051.1. Transcript Variant: This variant (4) has multiple differences in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC058003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGTTTT GTATCCAGAG ATTTATCAAC TTTGTTGTCA AACTAGAAAA CTCAAAACTC 
AAAGTTTCAA AGAACCAAAG GACTCTTCCT CTGGGCACCA CAAACTTCCC CTTCCTCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186980.1
CDS	105..827
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEFCIQRFINFVVKLENSKLKVSKNQRTLPLGTTNFPFLH

Target ORF information:

RefSeq Version	NM_001200051.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200051.2

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGTTTT GTATCCAGAG ATTTATCAAC TTTGTTGTCA AACTAGAAAA CTCAAAACTC 
AAAGTTTCAA AGAACCAAAG GACTCTTCCT CTGGGCACCA CAAACTTCCC CTTCCTCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_001200051.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186980.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu06180
Clone ID Related Accession (Same CDS sequence)	NM_001200051.2 , NM_001200051.1
Accession Version	NM_001200051.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB961482.1, BC058003.1 and AI249145.1. Transcript Variant: This variant (4) has multiple differences in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC058003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGTTTT GTATCCAGAG ATTTATCAAC TTTGTTGTCA AACTAGAAAA CTCAAAACTC 
AAAGTTTCAA AGAACCAAAG GACTCTTCCT CTGGGCACCA CAAACTTCCC CTTCCTCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186980.1
CDS	429..1151
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEFCIQRFINFVVKLENSKLKVSKNQRTLPLGTTNFPFLH

Target ORF information:

RefSeq Version	NM_001200051.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200051.1

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGTTTT GTATCCAGAG ATTTATCAAC TTTGTTGTCA AACTAGAAAA CTCAAAACTC 
AAAGTTTCAA AGAACCAAAG GACTCTTCCT CTGGGCACCA CAAACTTCCC CTTCCTCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_030769.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_110396.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08401
Clone ID Related Accession (Same CDS sequence)	NM_030769.2
Accession Version	NM_030769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB961482.1, AK313225.1 and AL355999.9. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2011 this sequence version replaced NM_030769.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK313225.1, SRR1803616.147797.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGGT 
TTTGGAGTGT CACAGACCAA AGCCATCATG ACTCTGGTCT CTGGGATTCC AATGGGCCCA 
CCCCGGCTTC CACTGCAGAA AGCCTCCAGG GAGTTTACTG ATAGTGCTGA AGCTAAACTG 
AAGAGCCTGG ATTTCCTTTC TTTCACTGAT TTAAAGGATG GAAACTTGGA AGCTGGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110396.1
CDS	429..1391
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLGFGVSQTKAIMTLVSGIPMGPPRLPLQKASREFTDSAEAKLKSLDFLSFTDLKDGNLEAGS

Target ORF information:

RefSeq Version	NM_030769.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030769.2

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGGT 
TTTGGAGTGT CACAGACCAA AGCCATCATG ACTCTGGTCT CTGGGATTCC AATGGGCCCA 
CCCCGGCTTC CACTGCAGAA AGCCTCCAGG GAGTTTACTG ATAGTGCTGA AGCTAAACTG 
AAGAGCCTGG ATTTCCTTTC TTTCACTGAT TTAAAGGATG GAAACTTGGA AGCTGGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_001200056.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186985.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07959
Clone ID Related Accession (Same CDS sequence)	NM_001200056.1 , NM_001200056.2
Accession Version	NM_001200056.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513344.15, BC042003.1, AK313225.1 and AL355999.9. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC042003.1, SRR1660809.119925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGAA 
AACTCAAAAC TCAAAGTTTC AAAGAACCAA AGGACTCTTC CTCTGGGCAC CACAAACTTC 
CCCTTCCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186985.1
CDS	429..1283
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLENSKLKVSKNQRTLPLGTTNFPFLH

Target ORF information:

RefSeq Version	NM_001200056.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200056.1

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGAA 
AACTCAAAAC TCAAAGTTTC AAAGAACCAA AGGACTCTTC CTCTGGGCAC CACAAACTTC 
CCCTTCCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_001200056.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186985.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.

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CloneID	OHu07959
Clone ID Related Accession (Same CDS sequence)	NM_001200056.1 , NM_001200056.2
Accession Version	NM_001200056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC042003.1, AK313225.1 and AL355999.9. On Jun 2, 2019 this sequence version replaced NM_001200056.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.443922.1, SRR5189658.132867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGAA 
AACTCAAAAC TCAAAGTTTC AAAGAACCAA AGGACTCTTC CTCTGGGCAC CACAAACTTC 
CCCTTCCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186985.1
CDS	105..959
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLENSKLKVSKNQRTLPLGTTNFPFLH

Target ORF information:

RefSeq Version	NM_001200056.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200056.2

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTACCTAT 
AACTACCTGG GAAAAAAGAC AAACCAGATG TTGGAGGCTT TTGAACAAAA GGACTTCTCT 
TTAGCCCTGA ACTATCAGTT TTGTATCCAG AGATTTATCA ACTTTGTTGT CAAACTAGAA 
AACTCAAAAC TCAAAGTTTC AAAGAACCAA AGGACTCTTC CTCTGGGCAC CACAAACTTC 
CCCTTCCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_001200050.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186979.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 2, mRNA.

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CloneID	OHu08488
Clone ID Related Accession (Same CDS sequence)	NM_001200050.1
Accession Version	NM_001200050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB961482.1, AY336748.1 and AL355999.9. On Aug 31, 2013 this sequence version replaced XM_005245505.1. Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate splice site in the coding region and initiates translation at a downstream start site, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY336748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGGG CCCCCGGGAT CCTGGCTTCT TGGAGAAGAG CCCCCTCCCT TTCTGTGCAG 
AAGGGCAGCC AAACTGCTAG ACACACCTGC CACCCAGAGG TTCCGCTGGG AAACTGCTTC 
TTACCTGTCT ACAAGGCCAG TCCCCTCACA GTTACCCGTC TTTGGGCTGA AAGGCTGGAT 
CAGGTGATAA TTCACGTAGG AGCACTGAGC TTGAAGGAGT CACAGGAACT GGCCCAACAT 
GCAGCAGAAA TAGGAGCTGA TGGCATCGCT GTCATTGCAC CGTTCTTCCT CAAGCCATGG 
ACCAAAGATA TCCTGATTAA TTTCCTAAAG GAAGTGGCTG CTGCCGCCCC TGCCCTGCCA 
TTTTATTACT ATCACATTCC TGCCTTGACA GGGGTAAAGA TTCGTGCTGA GGAGTTGTTG 
GATGGGATTC TGGATAAGAT CCCCACCTTC CAAGGGCTGA AATTCAGTGA TACAGATCTC 
TTAGACTTCG GGCAATGTGT TGATCAGAAT CGCCAGCAAC AGTTTGCTTT CCTTTTTGGG 
GTGGATGAGC AACTGTTGAG TGCTCTGGTG ATGGGAGCAA CTGGAGCAGT GGGCAGTACC 
TATAACTACC TGGGAAAAAA GACAAACCAG ATGTTGGAGG CTTTTGAACA AAAGGACTTC 
TCTTTAGCCC TGAACTATCA GTTTTGTATC CAGAGATTTA TCAACTTTGT TGTCAAACTA 
GGTTTTGGAG TGTCACAGAC CAAAGCCATC ATGACTCTGG TCTCTGGGAT TCCAATGGGC 
CCACCCCGGC TTCCACTGCA GAAAGCCTCC AGGGAGTTTA CTGATAGTGC TGAAGCTAAA 
CTGAAGAGCC TGGATTTCCT TTCTTTCACT GATTTAAAGG ATGGAAACTT GGAAGCTGGT 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186979.1
CDS	646..1551
Translation	MSRAPGILASWRRAPSLSVQKGSQTARHTCHPEVPLGNCFLPVYKASPLTVTRLWAERLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLGFGVSQTKAIMTLVSGIPMGPPRLPLQKASREFTDSAEAKLKSLDFLSFTDLKDGNLEAGS

Target ORF information:

RefSeq Version	NM_001200050.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200050.1

ORF Insert Sequence:

ATGAGCAGGG CCCCCGGGAT CCTGGCTTCT TGGAGAAGAG CCCCCTCCCT TTCTGTGCAG 
AAGGGCAGCC AAACTGCTAG ACACACCTGC CACCCAGAGG TTCCGCTGGG AAACTGCTTC 
TTACCTGTCT ACAAGGCCAG TCCCCTCACA GTTACCCGTC TTTGGGCTGA AAGGCTGGAT 
CAGGTGATAA TTCACGTAGG AGCACTGAGC TTGAAGGAGT CACAGGAACT GGCCCAACAT 
GCAGCAGAAA TAGGAGCTGA TGGCATCGCT GTCATTGCAC CGTTCTTCCT CAAGCCATGG 
ACCAAAGATA TCCTGATTAA TTTCCTAAAG GAAGTGGCTG CTGCCGCCCC TGCCCTGCCA 
TTTTATTACT ATCACATTCC TGCCTTGACA GGGGTAAAGA TTCGTGCTGA GGAGTTGTTG 
GATGGGATTC TGGATAAGAT CCCCACCTTC CAAGGGCTGA AATTCAGTGA TACAGATCTC 
TTAGACTTCG GGCAATGTGT TGATCAGAAT CGCCAGCAAC AGTTTGCTTT CCTTTTTGGG 
GTGGATGAGC AACTGTTGAG TGCTCTGGTG ATGGGAGCAA CTGGAGCAGT GGGCAGTACC 
TATAACTACC TGGGAAAAAA GACAAACCAG ATGTTGGAGG CTTTTGAACA AAAGGACTTC 
TCTTTAGCCC TGAACTATCA GTTTTGTATC CAGAGATTTA TCAACTTTGT TGTCAAACTA 
GGTTTTGGAG TGTCACAGAC CAAAGCCATC ATGACTCTGG TCTCTGGGAT TCCAATGGGC 
CCACCCCGGC TTCCACTGCA GAAAGCCTCC AGGGAGTTTA CTGATAGTGC TGAAGCTAAA 
CTGAAGAGCC TGGATTTCCT TTCTTTCACT GATTTAAAGG ATGGAAACTT GGAAGCTGGT 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPL ( NM_001200052.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186981.1 Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu08512
Clone ID Related Accession (Same CDS sequence)	NM_001200052.1
Accession Version	NM_001200052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	N-acetylneuraminate lyase isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB961482.1, AL525867.3 and AL355999.9. Transcript Variant: This variant (5) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (5) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.135896.1, SRR1803617.240070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTTTTGTA 
TCCAGAGATT TATCAACTTT GTTGTCAAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186981.1
CDS	429..1121
Translation	MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSFVSRDLSTLLSN

Target ORF information:

RefSeq Version	NM_001200052.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acetylneuraminate pyruvate lyase (NPL), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200052.1

ORF Insert Sequence:

ATGGCCTTCC CAAAGAAGAA ACTTCAGGGT CTTGTGGCTG CAACCATCAC GCCAATGACT 
GAGAATGGAG AAATCAACTT TTCAGTAATT GGTCAGTATG TGGATTATCT TGTGAAAGAA 
CAGGGAGTGA AGAACATTTT TGTGAATGGC ACAACAGGAG AAGGCCTGTC CCTGAGCGTC 
TCAGAGCGTC GCCAGGTTGC AGAGGAGTGG GTGACAAAAG GGAAGGACAA GCTGGATCAG 
GTGATAATTC ACGTAGGAGC ACTGAGCTTG AAGGAGTCAC AGGAACTGGC CCAACATGCA 
GCAGAAATAG GAGCTGATGG CATCGCTGTC ATTGCACCGT TCTTCCTCAA GCCATGGACC 
AAAGATATCC TGATTAATTT CCTAAAGGAA GTGGCTGCTG CCGCCCCTGC CCTGCCATTT 
TATTACTATC ACATTCCTGC CTTGACAGGG GTAAAGATTC GTGCTGAGGA GTTGTTGGAT 
GGGATTCTGG ATAAGATCCC CACCTTCCAA GGGCTGAAAT TCAGTGATAC AGATCTCTTA 
GACTTCGGGC AATGTGTTGA TCAGAATCGC CAGCAACAGT TTGCTTTCCT TTTTGGGGTG 
GATGAGCAAC TGTTGAGTGC TCTGGTGATG GGAGCAACTG GAGCAGTGGG CAGTTTTGTA 
TCCAGAGATT TATCAACTTT GTTGTCAAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.
The Journal of biological chemistry287(34)28865-81(2012 Aug)
Bergfeld AK,Pearce OM,Diaz SL,Pham T,Varki A

Homology modeling and molecular dynamics study on N-acetylneuraminate lyase.
Journal of molecular modeling15(3)323-8(2009 Mar)
Chu HY,Zheng QC,Zhao YS,Zhang HX

A novel splice variant of human gene NPL, mainly expressed in human liver, kidney and peripheral blood leukocyte.
DNA sequence : the journal of DNA sequencing and mapping16(2)137-42(2005 Apr)
Wu M,Gu S,Xu J,Zou X,Zheng H,Jin Z,Xie Y,Ji C,Mao Y

Characterization of a sialate pyruvate-lyase in the cytosol of human erythrocytes.
Biochimie84(7)655-60(2002 Jul)
Bulai T,Bratosin D,Artenie V,Montreuil J

The sialate-pyruvate lyase from pig kidney. Elucidation of the primary structure and expression of recombinant enzyme activity.
European journal of biochemistry268(24)6473-86(2001 Dec)
Traving C,Bruse P,W?chter A,Schauer R

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NPL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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