SSPN cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSPN
Entrez Gene ID	8082
Full Name	sarcospan
Synonyms	DAGA5,KRAG,NSPN,SPN1,SPN2
General protein information	Preferred Names sarcospan Names sarcospan K-ras oncogene-associated protein Kras oncogene-associated kirsten-ras-associated protein microspan nanospan sarcospan (Kras oncogene-associated gene)
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 12 Map Location: 12p12.1
Summary	This gene encodes a member of the dystrophin-glycoprotein complex (DGC). The DGC spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Two alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008].
Disorder MIM:	601599

mRNA and Protein(s)

mRNA	Protein	Name
XM_011520855.2	XP_011519157.1	sarcospan isoform X1
XM_011520855.1	XP_011519157.1	sarcospan isoform X1
XM_011520854.1	XP_011519156.1	sarcospan isoform X1
XM_011520853.2	XP_011519155.1	sarcospan isoform X1
NM_001135823.1	NP_001129295.1	sarcospan isoform 2
NM_005086.4	NP_005077.2	sarcospan isoform 1
XM_011520853.3	XP_011519155.1	sarcospan isoform X1
XM_011520854.2	XP_011519156.1	sarcospan isoform X1
XM_011520853.1	XP_011519155.1	sarcospan isoform X1

Pathways from BioSystems

Homologies

	SSPN	XP_001102485.1
Rattus norvegicus (Norway rat)	Sspn	NP_001102725.1
Canis lupus familiaris (dog)	SSPN	XP_003433567.2
Gallus gallus (chicken)	SSPN	XP_416438.1
Danio rerio (zebrafish)	sspn	NP_001032669.1
Homo sapiens (human)	SSPN	NP_005077.2
Pan troglodytes (chimpanzee)	SSPN	XP_001144827.1
Mus musculus (house mouse)	Sspn	NP_034786.1
Bos taurus (cattle)	SSPN	NP_001179934.1

Gene Ontology
Bibliography

Related articles in PubMed

Nkx2-5 and Sarcospan genetically interact in the development of the muscular ventricular septum of the heart.
Panzer AA, Regmi SD, Cormier D, Danzo MT, Chen ID, Winston JB, Hutchinson AK, Salm D, Schulkey CE, Cochran RS, Wilson DB, Jay PY
Scientific reports746438(2017 Apr)

High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.
Gibbs EM, Marshall JL, Ma E, Nguyen TM, Hong G, Lam JS, Spencer MJ, Crosbie-Watson RH
Human molecular genetics25(24)5395-5406(2016 12)

Differential Expression between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia.
Chew EG, Tan JH, Bahta AW, Ho BS, Liu X, Lim TC, Sia YY, Bigliardi PL, Heilmann S, Wan AC, N?then MM, Philpott MP, Hillmer AM
The Journal of investigative dermatology136(8)1559-67(2016 Aug)

Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distribution.
Heid IM, Jackson AU, Randall JC, Winkler TW, Qi L, Steinthorsdottir V, Thorleifsson G, Zillikens MC, Speliotes EK, M?gi R, Workalemahu T, White CC, Bouatia-Naji N, Harris TB, Berndt SI, Ingelsson E, Willer CJ, Weedon MN, Luan J, Vedantam S, Esko T, Kilpel?inen TO, Kutalik Z, Li S, Monda KL, Dixon AL, Holmes CC, Kaplan LM, Liang L, Min JL, Moffatt MF, Molony C, Nicholson G, Schadt EE, Zondervan KT, Feitosa MF, Ferreira T, Lango Allen H, Weyant RJ, Wheeler E, Wood AR, , Estrada K, Goddard ME, Lettre G, Mangino M, Nyholt DR, Purcell S, Smith AV, Visscher PM, Yang J, McCarroll SA, Nemesh J, Voight BF, Absher D, Amin N, Aspelund T, Coin L, Glazer NL, Hayward C, Heard-Costa NL, Hottenga JJ, Johansson A, Johnson T, Kaakinen M, Kapur K, Ketkar S, Knowles JW, Kraft P, Kraja AT, Lamina C, Leitzmann MF, McKnight B, Morris AP, Ong KK, Perry JR, Peters MJ, Polasek O, Prokopenko I, Rayner NW, Ripatti S, Rivadeneira F, Robertson NR, Sanna S, Sovio U, Surakka I, Teumer A, van Wingerden S, Vitart V, Zhao JH, Cavalcanti-Proen?a C, Chines PS, Fisher E, Kulzer JR, Lecoeur C, Narisu N, Sandholt C, Scott LJ, Silander K, Stark K, Tammesoo ML, Teslovich TM, Timpson NJ, Watanabe RM, Welch R, Chasman DI, Cooper MN, Jansson JO, Kettunen J, Lawrence RW, Pellikka N, Perola M, Vandenput L, Alavere H, Almgren P, Atwood LD, Bennett AJ, Biffar R, Bonnycastle LL, Bornstein SR, Buchanan TA, Campbell H, Day IN, Dei M, D?rr M, Elliott P, Erdos MR, Eriksson JG, Freimer NB, Fu M, Gaget S, Geus EJ, Gjesing AP, Grallert H, Gr?ssler J, Groves CJ, Guiducci C, Hartikainen AL, Hassanali N, Havulinna AS, Herzig KH, Hicks AA, Hui J, Igl W, Jousilahti P, Jula A, Kajantie E, Kinnunen L, Kolcic I, Koskinen S, Kovacs P, Kroemer HK, Krzelj V, Kuusisto J, Kvaloy K, Laitinen J, Lantieri O, Lathrop GM, Lokki ML, Luben RN, Ludwig B, McArdle WL, McCarthy A, Morken MA, Nelis M, Neville MJ, Par? G, Parker AN, Peden JF, Pichler I, Pietil?inen KH, Platou CG, Pouta A, Ridderstr?le M, Samani NJ, Saramies J, Sinisalo J, Smit JH, Strawbridge RJ, Stringham HM, Swift AJ, Teder-Laving M, Thomson B, Usala G, van Meurs JB, van Ommen GJ, Vatin V, Volpato CB, Wallaschofski H, Walters GB, Widen E, Wild SH, Willemsen G, Witte DR, Zgaga L, Zitting P, Beilby JP, James AL, K?h?nen M, Lehtim?ki T, Nieminen MS, Ohlsson C, Palmer LJ, Raitakari O, Ridker PM, Stumvoll M, T?njes A, Viikari J, Balkau B, Ben-Shlomo Y, Bergman RN, Boeing H, Smith GD, Ebrahim S, Froguel P, Hansen T, Hengstenberg C, Hveem K, Isomaa B, J?rgensen T, Karpe F, Khaw KT, Laakso M, Lawlor DA, Marre M, Meitinger T, Metspalu A, Midthjell K, Pedersen O, Salomaa V, Schwarz PE, Tuomi T, Tuomilehto J, Valle TT, Wareham NJ, Arnold AM, Beckmann JS, Bergmann S, Boerwinkle E, Boomsma DI, Caulfield MJ, Collins FS, Eiriksdottir G, Gudnason V, Gyllensten U, Hamsten A, Hattersley AT, Hofman A, Hu FB, Illig T, Iribarren C, Jarvelin MR, Kao WH, Kaprio J, Launer LJ, Munroe PB, Oostra B, Penninx BW, Pramstaller PP, Psaty BM, Quertermous T, Rissanen A, Rudan I, Shuldiner AR, Soranzo N, Spector TD, Syvanen AC, Uda M, Uitterlinden A, V?lzke H, Vollenweider P, Wilson JF, Witteman JC, Wright AF, Abecasis GR, Boehnke M, Borecki IB, Deloukas P, Frayling TM, Groop LC, Haritunians T, Hunter DJ, Kaplan RC, North KE, O'Connell JR, Peltonen L, Schlessinger D, Strachan DP, Hirschhorn JN, Assimes TL, Wichmann HE, Thorsteinsdottir U, van Duijn CM, Stefansson K, Cupples LA, Loos RJ, Barroso I, McCarthy MI, Fox CS, Mohlke KL, Lindgren CM
Nature genetics42(11)949-60(2010 Nov)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When human SSPN is expressed at three-fold levels in mdx mice, this increase in adhesion complex abundance improves muscle membrane stability, preventing many of the histopathological changes associated with Duchenne muscular dystrophy
Title: High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.

Our data suggest TWIST1 and SSPN to be the functionally relevant androgenetic alopecia genes at the 7p21.1 and 12p12.1 risk loci, respectively.
Title: Differential Expression between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distribution.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following SSPN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSPN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18779	NM_001135823.1 Latest version!	Homo sapiens sarcospan (SSPN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18939	NM_005086.4 Latest version!	Homo sapiens sarcospan (SSPN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520855.2 Latest version!	Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520853.3 Latest version!	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520854.2 Latest version!	Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18779	XM_011520855.1	Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520853.2	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520854.1	Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18779	XM_011520853.1	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSPN ( NM_001135823.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001129295.1 Homo sapiens sarcospan (SSPN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	NM_001135823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-15
Organism	Homo sapiens(human)
Product	sarcospan isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA073350.1, AK092925.1, AC022509.21 and CA419006.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) uses a different segment for its 5' UTR and lacks a 5' coding segment which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.28010.1, SRR1660805.131462.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129295.1
CDS	227..649
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	NM_001135823.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135823.1

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( NM_005086.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005077.2 Homo sapiens sarcospan (SSPN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18939
Clone ID Related Accession (Same CDS sequence)	NM_005086.4
Accession Version	NM_005086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	sarcospan isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP341300.1, AL136756.1, AC022509.21 and CA419006.1. This sequence is a reference standard in the RefSeqGene project. On Oct 16, 2008 this sequence version replaced NM_005086.3. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP341300.1, AL136756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGGCAAGA ACAAGCAGCC ACGCGGCCAG CAGAGGCAGG GGGGCCCGCC GGCCGCGGAC 
GCCGCTGGGC CCGACGACAT GGAGCCGAAG AAGGGCACGG GGGCCCCCAA GGAGTGCGGG 
GAGGAGGAGC CCCGGACCTG CTGCGGCTGC CGGTTCCCGC TGCTGCTCGC CCTGCTGCAG 
CTGGCCCTGG GCATCGCCGT GACCGTGGTG GGCTTCCTCA TGGCGAGCAT CAGCTCCTCC 
CTGCTAGTCA GGGACACTCC ATTTTGGGCT GGGATCATTG TCTGCTTAGT GGCCTATCTT 
GGCTTGTTTA TGCTTTGTGT CTCATATCAG GTTGACGAAC GGACATGTAT TCAATTTTCT 
ATGAAACTGT TATACTTTCT GCTGAGTGCC CTGGGCCTGA CGGTCTGTGT GCTGGCCGTG 
GCCTTTGCCG CCCACCACTA TTCGCAGCTC ACACAGTTTA CCTGTGAGAC CACACTCGAC 
TCTTGCCAGT GCAAACTGCC CTCCTCGGAG CCGCTCAGCA GGACCTTTGT TTACCGGGAT 
GTGACGGACT GTACCAGCGT CACTGGCACT TTCAAACTGT TCTTACTCAT CCAGATGATT 
CTTAATTTGG TCTGCGGCCT TGTGTGCTTG TTGGCCTGCT TTGTGATGTG GAAACATAGG 
TACCAGGTCT TCTATGTGGG TGTCAGGATA TGCTCCCTCA CGGCTTCCGA AGGCCCCCAG 
CAAAAGATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005077.2
CDS	101..832
Translation	MGKNKQPRGQQRQGGPPAADAAGPDDMEPKKGTGAPKECGEEEPRTCCGCRFPLLLALLQLALGIAVTVVGFLMASISSSLLVRDTPFWAGIIVCLVAYLGLFMLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	NM_005086.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005086.4

ORF Insert Sequence:

ATGGGCAAGA ACAAGCAGCC ACGCGGCCAG CAGAGGCAGG GGGGCCCGCC GGCCGCGGAC 
GCCGCTGGGC CCGACGACAT GGAGCCGAAG AAGGGCACGG GGGCCCCCAA GGAGTGCGGG 
GAGGAGGAGC CCCGGACCTG CTGCGGCTGC CGGTTCCCGC TGCTGCTCGC CCTGCTGCAG 
CTGGCCCTGG GCATCGCCGT GACCGTGGTG GGCTTCCTCA TGGCGAGCAT CAGCTCCTCC 
CTGCTAGTCA GGGACACTCC ATTTTGGGCT GGGATCATTG TCTGCTTAGT GGCCTATCTT 
GGCTTGTTTA TGCTTTGTGT CTCATATCAG GTTGACGAAC GGACATGTAT TCAATTTTCT 
ATGAAACTGT TATACTTTCT GCTGAGTGCC CTGGGCCTGA CGGTCTGTGT GCTGGCCGTG 
GCCTTTGCCG CCCACCACTA TTCGCAGCTC ACACAGTTTA CCTGTGAGAC CACACTCGAC 
TCTTGCCAGT GCAAACTGCC CTCCTCGGAG CCGCTCAGCA GGACCTTTGT TTACCGGGAT 
GTGACGGACT GTACCAGCGT CACTGGCACT TTCAAACTGT TCTTACTCAT CCAGATGATT 
CTTAATTTGG TCTGCGGCCT TGTGTGCTTG TTGGCCTGCT TTGTGATGTG GAAACATAGG 
TACCAGGTCT TCTATGTGGG TGTCAGGATA TGCTCCCTCA CGGCTTCCGA AGGCCCCCAG 
CAAAAGATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520855.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519157.1 Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519157.1
CDS	76..498
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520855.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520855.2

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520855.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519157.1 Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520855.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sarcospan isoform X1
Comment

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519157.1
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520855.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520855.1

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520854.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519156.1 Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520854.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sarcospan isoform X1
Comment

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519156.1
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520854.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520854.1

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520853.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519155.1 Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520853.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519155.1
CDS	160..582
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520853.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520853.2

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520853.3 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519155.1 Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519155.1
CDS	233..655
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520853.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520853.3

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520854.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519156.1 Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519156.1
CDS	334..756
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520854.2

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSPN ( XM_011520853.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011519155.1 Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18779
Clone ID Related Accession (Same CDS sequence)	XM_011520855.2 , XM_011520855.1 , XM_011520854.1 , XM_011520853.2 , NM_001135823.1 , XM_011520853.3 , XM_011520854.2 , XM_011520853.1
Accession Version	XM_011520853.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sarcospan isoform X1
Comment

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519155.1
Translation	MLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI

Target ORF information:

RefSeq Version	XM_011520853.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sarcospan (SSPN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520853.1

ORF Insert Sequence:

ATGCTTTGTG TCTCATATCA GGTTGACGAA CGGACATGTA TTCAATTTTC TATGAAACTG 
TTATACTTTC TGCTGAGTGC CCTGGGCCTG ACGGTCTGTG TGCTGGCCGT GGCCTTTGCC 
GCCCACCACT ATTCGCAGCT CACACAGTTT ACCTGTGAGA CCACACTCGA CTCTTGCCAG 
TGCAAACTGC CCTCCTCGGA GCCGCTCAGC AGGACCTTTG TTTACCGGGA TGTGACGGAC 
TGTACCAGCG TCACTGGCAC TTTCAAACTG TTCTTACTCA TCCAGATGAT TCTTAATTTG 
GTCTGCGGCC TTGTGTGCTT GTTGGCCTGC TTTGTGATGT GGAAACATAG GTACCAGGTC 
TTCTATGTGG GTGTCAGGAT ATGCTCCCTC ACGGCTTCCG AAGGCCCCCA GCAAAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nkx2-5 and Sarcospan genetically interact in the development of the muscular ventricular septum of the heart.
Scientific reports746438(2017 Apr)
Panzer AA,Regmi SD,Cormier D,Danzo MT,Chen ID,Winston JB,Hutchinson AK,Salm D,Schulkey CE,Cochran RS,Wilson DB,Jay PY

High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.
Human molecular genetics25(24)5395-5406(2016 12)
Gibbs EM,Marshall JL,Ma E,Nguyen TM,Hong G,Lam JS,Spencer MJ,Crosbie-Watson RH

Differential Expression between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia.
The Journal of investigative dermatology136(8)1559-67(2016 Aug)
Chew EG,Tan JH,Bahta AW,Ho BS,Liu X,Lim TC,Sia YY,Bigliardi PL,Heilmann S,Wan AC,N?then MM,Philpott MP,Hillmer AM

Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distribution.
Nature genetics42(11)949-60(2010 Nov)
Heid IM,Jackson AU,Randall JC,Winkler TW,Qi L,Steinthorsdottir V,Thorleifsson G,Zillikens MC,Speliotes EK,M?gi R,Workalemahu T,White CC,Bouatia-Naji N,Harris TB,Berndt SI,Ingelsson E,Willer CJ,Weedon MN,Luan J,Vedantam S,Esko T,Kilpel?inen TO,Kutalik Z,Li S,Monda KL,Dixon AL,Holmes CC,Kaplan LM,Liang L,Min JL,Moffatt MF,Molony C,Nicholson G,Schadt EE,Zondervan KT,Feitosa MF,Ferreira T,Lango Allen H,Weyant RJ,Wheeler E,Wood AR,,Estrada K,Goddard ME,Lettre G,Mangino M,Nyholt DR,Purcell S,Smith AV,Visscher PM,Yang J,McCarroll SA,Nemesh J,Voight BF,Absher D,Amin N,Aspelund T,Coin L,Glazer NL,Hayward C,Heard-Costa NL,Hottenga JJ,Johansson A,Johnson T,Kaakinen M,Kapur K,Ketkar S,Knowles JW,Kraft P,Kraja AT,Lamina C,Leitzmann MF,McKnight B,Morris AP,Ong KK,Perry JR,Peters MJ,Polasek O,Prokopenko I,Rayner NW,Ripatti S,Rivadeneira F,Robertson NR,Sanna S,Sovio U,Surakka I,Teumer A,van Wingerden S,Vitart V,Zhao JH,Cavalcanti-Proen?a C,Chines PS,Fisher E,Kulzer JR,Lecoeur C,Narisu N,Sandholt C,Scott LJ,Silander K,Stark K,Tammesoo ML,Teslovich TM,Timpson NJ,Watanabe RM,Welch R,Chasman DI,Cooper MN,Jansson JO,Kettunen J,Lawrence RW,Pellikka N,Perola M,Vandenput L,Alavere H,Almgren P,Atwood LD,Bennett AJ,Biffar R,Bonnycastle LL,Bornstein SR,Buchanan TA,Campbell H,Day IN,Dei M,D?rr M,Elliott P,Erdos MR,Eriksson JG,Freimer NB,Fu M,Gaget S,Geus EJ,Gjesing AP,Grallert H,Gr?ssler J,Groves CJ,Guiducci C,Hartikainen AL,Hassanali N,Havulinna AS,Herzig KH,Hicks AA,Hui J,Igl W,Jousilahti P,Jula A,Kajantie E,Kinnunen L,Kolcic I,Koskinen S,Kovacs P,Kroemer HK,Krzelj V,Kuusisto J,Kvaloy K,Laitinen J,Lantieri O,Lathrop GM,Lokki ML,Luben RN,Ludwig B,McArdle WL,McCarthy A,Morken MA,Nelis M,Neville MJ,Par? G,Parker AN,Peden JF,Pichler I,Pietil?inen KH,Platou CG,Pouta A,Ridderstr?le M,Samani NJ,Saramies J,Sinisalo J,Smit JH,Strawbridge RJ,Stringham HM,Swift AJ,Teder-Laving M,Thomson B,Usala G,van Meurs JB,van Ommen GJ,Vatin V,Volpato CB,Wallaschofski H,Walters GB,Widen E,Wild SH,Willemsen G,Witte DR,Zgaga L,Zitting P,Beilby JP,James AL,K?h?nen M,Lehtim?ki T,Nieminen MS,Ohlsson C,Palmer LJ,Raitakari O,Ridker PM,Stumvoll M,T?njes A,Viikari J,Balkau B,Ben-Shlomo Y,Bergman RN,Boeing H,Smith GD,Ebrahim S,Froguel P,Hansen T,Hengstenberg C,Hveem K,Isomaa B,J?rgensen T,Karpe F,Khaw KT,Laakso M,Lawlor DA,Marre M,Meitinger T,Metspalu A,Midthjell K,Pedersen O,Salomaa V,Schwarz PE,Tuomi T,Tuomilehto J,Valle TT,Wareham NJ,Arnold AM,Beckmann JS,Bergmann S,Boerwinkle E,Boomsma DI,Caulfield MJ,Collins FS,Eiriksdottir G,Gudnason V,Gyllensten U,Hamsten A,Hattersley AT,Hofman A,Hu FB,Illig T,Iribarren C,Jarvelin MR,Kao WH,Kaprio J,Launer LJ,Munroe PB,Oostra B,Penninx BW,Pramstaller PP,Psaty BM,Quertermous T,Rissanen A,Rudan I,Shuldiner AR,Soranzo N,Spector TD,Syvanen AC,Uda M,Uitterlinden A,V?lzke H,Vollenweider P,Wilson JF,Witteman JC,Wright AF,Abecasis GR,Boehnke M,Borecki IB,Deloukas P,Frayling TM,Groop LC,Haritunians T,Hunter DJ,Kaplan RC,North KE,O'Connell JR,Peltonen L,Schlessinger D,Strachan DP,Hirschhorn JN,Assimes TL,Wichmann HE,Thorsteinsdottir U,van Duijn CM,Stefansson K,Cupples LA,Loos RJ,Barroso I,McCarthy MI,Fox CS,Mohlke KL,Lindgren CM

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

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SSPN cDNA ORF clone, Homo sapiens(Human)

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Clones

gRNAs

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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