COL18A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL18A1
Entrez Gene ID	80781
Full Name	collagen type XVIII alpha 1 chain
Synonyms	KNO,KNO1,KS
General protein information	Preferred Names collagen type XVIII alpha 1 chain Names collagen alpha-1(XVIII) chain antiangiogenic agent collagen alpha-1(XVIII) chain isoform 1 preproprotein collagen, type XVIII, alpha 1 endostatin multi-functional protein MFP
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.3
Summary	This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].
Disorder MIM:	120328
Disorder Html:	Knobloch syndrome, type 1, 267750 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130444.2	NP_569711.2	collagen alpha-1(XVIII) chain isoform 3 preproprotein
NM_030582.3	NP_085059.2	collagen alpha-1(XVIII) chain isoform 1 preproprotein
NM_130445.3	NP_569712.2	collagen alpha-1(XVIII) chain isoform 2 preproprotein
NM_130444.3	NP_569711.2	collagen alpha-1(XVIII) chain isoform 3 preproprotein
NM_030582.4	NP_085059.2	collagen alpha-1(XVIII) chain isoform 1 preproprotein
NM_130445.4	NP_569712.2	collagen alpha-1(XVIII) chain isoform 2 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col18a1	NP_445941.2
Pan troglodytes (chimpanzee)	COL18A1	XP_531500.4
Homo sapiens (human)	COL18A1	NP_085059.2
	COL18A1	XP_002803149.1
Mus musculus (house mouse)	Col18a1	NP_001103461.1
Xenopus tropicalis (tropical clawed frog)	col18a1	NP_989236.1
Gallus gallus (chicken)	COL18A1	NP_989495.1

Gene Ontology
Bibliography

Related articles in PubMed

Familial epilepsy with anterior polymicrogyria as a presentation of COL18A1 mutations.
Corbett MA, Turner SJ, Gardner A, Silver J, Stankovich J, Leventer RJ, Derry CP, Carroll R, Ha T, Scheffer IE, Bahlo M, Jackson GD, Mackey DA, Berkovic SF, Gecz J
European journal of medical genetics60(8)437-443(2017 Aug)

Homozygosity mapping and whole exome sequencing reveal a novel homozygous COL18A1 mutation causing Knobloch syndrome.
Haghighi A, Tiwari A, Piri N, N?rnberg G, Saleh-Gohari N, Haghighi A, Neidhardt J, N?rnberg P, Berger W
PloS one9(11)e112747(2014)

The multiple functions of collagen XVIII in development and disease.
Seppinen L, Pihlajaniemi T
Matrix biology : journal of the International Society for Matrix Biology30(2)83-92(2011 Mar)

Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases.
Heljasvaara R, Nyberg P, Luostarinen J, Parikka M, Heikkil? P, Rehn M, Sorsa T, Salo T, Pihlajaniemi T
Experimental cell research307(2)292-304(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two COL18A1 variants co-segregated with familial epilepsy and anterior polymicrogyria in a family.
Title: Familial epilepsy with anterior polymicrogyria as a presentation of COL18A1 mutations.

Lower endostatin levels or a predominance of VEGF over endostatin are predictors of poor short-term prognosis of intracranial atherosclerotic stenosis.
Title: Correlation of Plasma Vascular Endothelial Growth Factor and Endostatin Levels with Symptomatic Intra- and Extracranial Atherosclerotic Stenosis in a Chinese Han Population.

Blood endostatin wasdddddddddddddddddddddddddf significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis.
Title: Noninvasive estimation of liver fibrosis in biopsy-proven hepatitis C virus-infected patients: angiogenic fibrogenic link.

Structural and biological functions of COL18A1, such as its requirement in the maintenance of basement membrane integrity and its emerging roles in regulating cell survival, stem or progenitor cell maintenance and differentiation and inflammation have been reported. (Review)
Title: Collagen XVIII in tissue homeostasis and dysregulation - Lessons learned from model organisms and human patients.

We further show that the SAGA deubiquitinase-activated gene Multiplexin (Mp) is required in glia for proper photoreceptor axon targeting. Mutations in the human ortholog of Mp, COL18A1, have been identified in a family with a SCA7-like progressive visual disorder, suggesting that defects in the expression of this gene in SCA7 patients could play a role in the retinal degeneration that is unique to this ataxia.
Title: Transcriptome Profiling Identifies Multiplexin as a Target of SAGA Deubiquitinase Activity in Glia Required for Precise Axon Guidance During Drosophila Visual Development.

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu72836	NM_130444.3 Latest version!	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu19786	NM_130445.4 Latest version!	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu19882	NM_030582.4 Latest version!	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
View Old Accession Versions >>
OHu72836	NM_130444.2	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu19882	NM_030582.3	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu19786	NM_130445.3	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL18A1 ( NM_130444.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569711.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

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CloneID	OHu72836
Clone ID Related Accession (Same CDS sequence)	NM_130444.2 , NM_130444.3
Accession Version	NM_130444.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Sep 25, 2008 this sequence version replaced NM_130444.1. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 14614989 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC 
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA 
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC 
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC 
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC 
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT 
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC 
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC 
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC 
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC 
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG 
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT 
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG 
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG 
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC 
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA 
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC 
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC 
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA 
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA 
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG 
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG 
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT 
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG 
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG 
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG 
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT 
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA 
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC 
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC 
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC 
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC 
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA 
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG 
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT 
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA 
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT 
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA 
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA 
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA 
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA 
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC 
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC 
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC 
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC 
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC 
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG 
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC 
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG 
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA 
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT 
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG 
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA 
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC 
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA 
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG 
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG 
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC 
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC 
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC 
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT 
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA 
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG 
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC 
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC 
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC 
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG 
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG 
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG 
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG 
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG 
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC 
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT 
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC 
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG 
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG 
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC 
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569711.2
CDS	22..5277
Translation	MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADTTTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKGIRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGAHTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSGRASLSSLLGGAPPWGSLQDPDSQGLSPAAAAPSQQLQRPDVRLRTPLLHPLVMGSLGKHAAPSAFSSGLPGALSQVAVTTLTRDSGAWVSHVANSVGPGLANNSALLGADPEAPAGRCLPLPPSLPVCGHLGISRFWLPNHLHHESGEQVRAGARAWGGLLQTHCHPFLAWFFCLLLVPPCGSVPPPAPPPCCQFCEALQDACWSRLGGGRLPVACASLPTQEDGYCVLIGPAAERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_130444.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130444.2

ORF Insert Sequence:

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC 
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA 
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC 
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC 
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC 
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT 
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC 
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC 
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC 
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC 
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG 
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT 
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG 
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG 
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC 
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA 
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC 
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC 
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA 
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA 
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG 
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG 
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT 
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG 
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG 
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG 
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT 
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA 
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC 
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC 
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC 
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC 
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA 
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG 
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT 
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA 
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT 
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA 
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA 
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA 
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA 
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC 
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC 
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC 
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC 
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC 
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG 
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC 
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG 
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA 
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT 
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG 
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA 
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC 
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA 
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG 
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG 
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC 
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC 
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC 
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT 
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA 
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG 
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC 
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC 
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC 
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG 
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG 
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG 
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG 
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG 
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC 
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT 
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC 
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG 
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG 
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC 
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_130444.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569711.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

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CloneID	OHu72836
Clone ID Related Accession (Same CDS sequence)	NM_130444.2 , NM_130444.3
Accession Version	NM_130444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Nov 21, 2018 this sequence version replaced NM_130444.2. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC 
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA 
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC 
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC 
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC 
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT 
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC 
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC 
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC 
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC 
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG 
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT 
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG 
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG 
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC 
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA 
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC 
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC 
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA 
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA 
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG 
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG 
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT 
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG 
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG 
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG 
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT 
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA 
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC 
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC 
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC 
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC 
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA 
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG 
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT 
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA 
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT 
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA 
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA 
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA 
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA 
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC 
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC 
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC 
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC 
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC 
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG 
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC 
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG 
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA 
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT 
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG 
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA 
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC 
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA 
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG 
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG 
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC 
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC 
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC 
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT 
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA 
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG 
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC 
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC 
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC 
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG 
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG 
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG 
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG 
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG 
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC 
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT 
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC 
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG 
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG 
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC 
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569711.2
CDS	10..5265
Translation	MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADTTTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKGIRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGAHTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSGRASLSSLLGGAPPWGSLQDPDSQGLSPAAAAPSQQLQRPDVRLRTPLLHPLVMGSLGKHAAPSAFSSGLPGALSQVAVTTLTRDSGAWVSHVANSVGPGLANNSALLGADPEAPAGRCLPLPPSLPVCGHLGISRFWLPNHLHHESGEQVRAGARAWGGLLQTHCHPFLAWFFCLLLVPPCGSVPPPAPPPCCQFCEALQDACWSRLGGGRLPVACASLPTQEDGYCVLIGPAAERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_130444.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130444.3

ORF Insert Sequence:

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC 
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA 
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC 
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC 
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC 
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT 
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC 
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC 
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC 
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC 
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG 
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT 
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG 
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG 
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC 
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA 
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC 
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC 
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA 
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA 
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG 
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG 
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT 
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG 
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG 
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG 
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT 
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA 
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC 
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC 
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC 
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC 
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA 
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG 
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT 
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA 
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT 
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA 
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA 
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA 
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA 
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC 
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC 
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC 
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC 
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC 
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG 
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC 
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG 
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA 
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT 
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG 
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA 
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC 
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA 
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG 
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG 
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC 
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC 
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC 
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT 
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA 
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG 
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC 
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC 
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC 
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG 
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG 
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG 
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG 
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG 
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC 
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT 
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC 
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG 
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG 
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC 
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_130445.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569712.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

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CloneID	OHu19786
Clone ID Related Accession (Same CDS sequence)	NM_130445.3 , NM_130445.4
Accession Version	NM_130445.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Dec 14, 2014 this sequence version replaced NM_130445.2. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569712.2
CDS	95..4105
Translation	MAPRCPWPWPRRRRLLDVLAPLVLLLGVRAASAEPERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_130445.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130445.3

ORF Insert Sequence:

ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_130445.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_569712.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19786
Clone ID Related Accession (Same CDS sequence)	NM_130445.3 , NM_130445.4
Accession Version	NM_130445.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Nov 21, 2018 this sequence version replaced NM_130445.3. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569712.2
CDS	67..4077
Translation	MAPRCPWPWPRRRRLLDVLAPLVLLLGVRAASAEPERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_130445.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130445.4

ORF Insert Sequence:

ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_030582.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_085059.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

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CloneID	OHu19882
Clone ID Related Accession (Same CDS sequence)	NM_030582.3 , NM_030582.4
Accession Version	NM_030582.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2006 this sequence version replaced NM_030582.2. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085059.2
CDS	22..4572
Translation	MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADTTTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKGIRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGAHTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_030582.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030582.3

ORF Insert Sequence:

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_030582.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_085059.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

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CloneID	OHu19882
Clone ID Related Accession (Same CDS sequence)	NM_030582.3 , NM_030582.4
Accession Version	NM_030582.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVIII) chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2018 this sequence version replaced NM_030582.3. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085059.2
CDS	10..4560
Translation	MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADTTTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKGIRSFVQLWNDTVPTESLARAETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGAHTTEAGTLPAPTPSPPSLGRPWAPLTGPSVPPPSSERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Target ORF information:

RefSeq Version	NM_030582.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030582.4

ORF Insert Sequence:

ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT 
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC 
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT 
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG 
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC 
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG 
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC 
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC 
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG 
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG 
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT 
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC 
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA 
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG 
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA 
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC 
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT 
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC 
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC 
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA 
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG 
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC 
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG 
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC 
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG 
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA 
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA 
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC 
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG 
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT 
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC 
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG 
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC 
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC 
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA 
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA 
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG 
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT 
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC 
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT 
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA 
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG 
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG 
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA 
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT 
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT 
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG 
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG 
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA 
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC 
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG 
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA 
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC 
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG 
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC 
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC 
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC 
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG 
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC 
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG 
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC 
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG 
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC 
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC 
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC 
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG 
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC 
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC 
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA 
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC 
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC 
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG 
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC 
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC 
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Familial epilepsy with anterior polymicrogyria as a presentation of COL18A1 mutations.
European journal of medical genetics60(8)437-443(2017 Aug)
Corbett MA,Turner SJ,Gardner A,Silver J,Stankovich J,Leventer RJ,Derry CP,Carroll R,Ha T,Scheffer IE,Bahlo M,Jackson GD,Mackey DA,Berkovic SF,Gecz J

Homozygosity mapping and whole exome sequencing reveal a novel homozygous COL18A1 mutation causing Knobloch syndrome.
PloS one9(11)e112747(2014)
Haghighi A,Tiwari A,Piri N,N?rnberg G,Saleh-Gohari N,Haghighi A,Neidhardt J,N?rnberg P,Berger W

The multiple functions of collagen XVIII in development and disease.
Matrix biology : journal of the International Society for Matrix Biology30(2)83-92(2011 Mar)
Seppinen L,Pihlajaniemi T

Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases.
Experimental cell research307(2)292-304(2005 Jul)
Heljasvaara R,Nyberg P,Luostarinen J,Parikka M,Heikkil? P,Rehn M,Sorsa T,Salo T,Pihlajaniemi T

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COL18A1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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