COL18A1 cDNA ORF clone, Homo sapiens(human)

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu72836 NM_130444.3
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu19786 NM_130445.4
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $384.30
$549.00
OHu19882 NM_030582.4
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
View Old Accession Versions >>
OHu72836 NM_130444.2 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu19882 NM_030582.3 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu19786 NM_130445.3 Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu72836
    Clone ID Related Accession (Same CDS sequence) NM_130444.2 , NM_130444.3
    Accession Version NM_130444.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 3 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Sep 25, 2008 this sequence version replaced NM_130444.1. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 14614989 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
    TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
    CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
    ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
    TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
    AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
    GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
    CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
    GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
    TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
    CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
    AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
    GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
    CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
    CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
    AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
    GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
    TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
    CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
    GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
    ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
    GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
    CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
    GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
    ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
    GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
    GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
    GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
    GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
    CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
    CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
    GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
    GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
    GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
    CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
    CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
    GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
    GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
    GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
    CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
    GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
    GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
    CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
    GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
    GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
    CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
    CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
    CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
    AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
    GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
    GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
    GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
    CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
    GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
    CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
    GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
    GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
    GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
    CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
    CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
    CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
    CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
    GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
    GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
    CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
    GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
    CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
    CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
    GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
    GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
    TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
    CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
    AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
    CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
    ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
    AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
    CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
    TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_569711.2
    CDS22..5277
    Translation

    Target ORF information:

    RefSeq Version NM_130444.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
    TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
    CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
    ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
    TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
    AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
    GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
    CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
    GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
    TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
    CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
    AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
    GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
    CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
    CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
    AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
    GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
    TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
    CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
    GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
    ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
    GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
    CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
    GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
    ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
    GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
    GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
    GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
    GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
    CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
    CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
    GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
    GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
    GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
    CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
    CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
    GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
    GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
    GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
    CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
    GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
    GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
    CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
    GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
    GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
    CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
    CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
    CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
    AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
    GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
    GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
    GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
    CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
    GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
    CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
    GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
    GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
    GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
    CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
    CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
    CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
    CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
    GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
    GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
    CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
    GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
    CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
    CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
    GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
    GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
    TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
    CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
    AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
    CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
    ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
    AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
    CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
    TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72836
    Clone ID Related Accession (Same CDS sequence) NM_130444.2 , NM_130444.3
    Accession Version NM_130444.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 3 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Nov 21, 2018 this sequence version replaced NM_130444.2. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
    TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
    CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
    ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
    TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
    AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
    GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
    CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
    GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
    TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
    CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
    AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
    GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
    CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
    CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
    AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
    GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
    TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
    CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
    GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
    ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
    GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
    CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
    GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
    ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
    GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
    GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
    GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
    GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
    CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
    CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
    GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
    GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
    GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
    CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
    CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
    GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
    GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
    GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
    CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
    GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
    GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
    CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
    GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
    GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
    CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
    CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
    CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
    AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
    GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
    GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
    GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
    CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
    GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
    CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
    GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
    GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
    GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
    CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
    CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
    CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
    CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
    GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
    GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
    CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
    GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
    CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
    CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
    GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
    GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
    TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
    CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
    AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
    CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
    ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
    AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
    CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
    TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_569711.2
    CDS10..5265
    Translation

    Target ORF information:

    RefSeq Version NM_130444.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130444.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
    TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
    CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
    ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
    TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
    AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
    GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
    CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
    GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
    TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
    CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
    AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
    GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
    CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
    CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
    AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
    GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
    TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
    CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
    GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
    ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
    GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
    CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
    GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
    ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
    GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
    GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
    GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
    GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
    CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
    CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
    GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
    GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
    GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
    CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
    CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
    GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
    GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
    GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
    CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
    GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
    GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
    CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
    GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
    GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
    CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
    CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
    CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
    AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
    GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
    GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
    GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
    CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
    GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
    CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
    GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
    GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
    GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
    CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
    CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
    CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
    CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
    GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
    GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
    CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
    GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
    CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
    CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
    GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
    GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
    TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
    CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
    AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
    CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
    ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
    AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
    CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
    TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19786
    Clone ID Related Accession (Same CDS sequence) NM_130445.3 , NM_130445.4
    Accession Version NM_130445.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 2 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Dec 14, 2014 this sequence version replaced NM_130445.2. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
    CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_569712.2
    CDS95..4105
    Translation

    Target ORF information:

    RefSeq Version NM_130445.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130445.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
    CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19786
    Clone ID Related Accession (Same CDS sequence) NM_130445.3 , NM_130445.4
    Accession Version NM_130445.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 2 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Nov 21, 2018 this sequence version replaced NM_130445.3. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
    CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_569712.2
    CDS67..4077
    Translation

    Target ORF information:

    RefSeq Version NM_130445.4
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130445.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
    CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19882
    Clone ID Related Accession (Same CDS sequence) NM_030582.3 , NM_030582.4
    Accession Version NM_030582.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2006 this sequence version replaced NM_030582.2. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_085059.2
    CDS22..4572
    Translation

    Target ORF information:

    RefSeq Version NM_030582.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_030582.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19882
    Clone ID Related Accession (Same CDS sequence) NM_030582.3 , NM_030582.4
    Accession Version NM_030582.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product collagen alpha-1(XVIII) chain isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2018 this sequence version replaced NM_030582.3. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_085059.2
    CDS10..4560
    Translation

    Target ORF information:

    RefSeq Version NM_030582.4
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_030582.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
    GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
    GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
    ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
    GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
    GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
    ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
    GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
    CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
    CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
    CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
    GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
    GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
    CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
    GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
    CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
    GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
    CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
    GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
    GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
    GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
    GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
    CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
    ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
    GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
    ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
    CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
    TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
    GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
    CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
    CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
    GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
    TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
    GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
    CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
    CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
    GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
    GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
    GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
    GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
    TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
    AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
    GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
    GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
    GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
    TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
    GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
    GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
    AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
    TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
    GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
    CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
    TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
    CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
    TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
    AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
    CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
    CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
    CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
    CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
    ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
    CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
    GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
    GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
    CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
    AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
    GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
    CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
    GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
    GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
    GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
    TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
    GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
    CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
    CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
    GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.