PDHX cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDHX
Entrez Gene ID	8050
Full Name	pyruvate dehydrogenase complex component X
Synonyms	DLDBP,E3BP,OPDX,PDHXD,PDX1,proX
General protein information	Preferred Names pyruvate dehydrogenase complex component X Names pyruvate dehydrogenase protein X component, mitochondrial dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex lipoyl-containing pyruvate dehydrogenase complex component X pyruvate dehydrogenase complex, E3-binding protein subunit pyruvate dehydrogenase complex, lipoyl-containing component X
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p13
Summary	The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009].
Disorder MIM:	608769
Disorder Html:	Lacticacidemia due to PDX1 deficiency, 245349 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003477.3	NP_003468.2	pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
NM_001166158.1	NP_001159630.1	pyruvate dehydrogenase protein X component, mitochondrial isoform 3 precursor
XM_011520390.1	XP_011518692.1	pyruvate dehydrogenase protein X component, mitochondrial isoform X1
NM_003477.2	NP_003468.2	pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
NM_001135024.1	NP_001128496.1	pyruvate dehydrogenase protein X component, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pdhx	NP_780303.1
Gallus gallus (chicken)	PDHX	NP_001026358.1
Xenopus tropicalis (tropical clawed frog)	pdhx	XP_002937341.2
Pan troglodytes (chimpanzee)	PDHX	XP_001149489.1
	PDHX	XP_001109997.2
Bos taurus (cattle)	LOC517402	NP_001069219.1
Homo sapiens (human)	PDHX	NP_003468.2
Canis lupus familiaris (dog)	PDHX	XP_533153.2
Danio rerio (zebrafish)	pdhx	NP_956854.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R, Rowland EA, Kang Y, Shenk T, Cristea IM
Cell159(7)1615-25(2014 Dec)

Founder p.Arg 446* mutation in the PDHX gene explains over half of cases with congenital lactic acidosis in Roma children.
Ivanov IS, Azmanov DN, Ivanova MB, Chamova T, Pacheva IH, Panova MV, Song S, Morar B, Yordanova RV, Galabova FK, Sotkova IG, Linev AJ, Bitchev S, Shearwood AM, Kancheva D, Gabrikova D, Karcagi V, Guergueltcheva V, Geneva IE, Bozhinova V, Stoyanova VK, Kremensky I, Jordanova A, Savov A, Horvath R, Brown MA, Tournev I, Filipovska A, Kalaydjieva L
Molecular genetics and metabolism113(1-2)76-83(2014 Sep-Oct)

MicroRNA-26a regulates glucose metabolism by direct targeting PDHX in colorectal cancer cells.
Chen B, Liu Y, Jin X, Lu W, Liu J, Xia Z, Yuan Q, Zhao X, Xu N, Liang S
BMC cancer14443(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We propose testing for the R446* mutation in PDHX as a rapid first screening in Roma infants with metabolic acidosis.
Title: Founder p.Arg 446* mutation in the PDHX gene explains over half of cases with congenital lactic acidosis in Roma children.

MiR-26a regulates glucose metabolism of colorectal cancer cells by direct targeting PDHX.
Title: MicroRNA-26a regulates glucose metabolism by direct targeting PDHX in colorectal cancer cells.

New mutation in PDHX gene found in two unrelated patients with Pyruvate dehydrogenase deficiency.
Title: Pyruvate dehydrogenase deficiency caused by a new mutation of PDHX gene in two Moroccan patients.

genetic association with systemic lupus erythematosus to a haplotype between PDHX and CD44 was established.
Title: Identification of a systemic lupus erythematosus susceptibility locus at 11p13 between PDHX and CD44 in a multiethnic study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following PDHX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10429	NM_001166158.1 Latest version!	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu19013	NM_003477.3 Latest version!	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61608	XM_011520390.1 Latest version!	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu07230	NM_001135024.1 Latest version!	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$286.30 $409.00
View Old Accession Versions >>
OHu19013	NM_003477.2	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDHX ( NM_001166158.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159630.1 Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10429
Clone ID Related Accession (Same CDS sequence)	NM_001166158.1
Accession Version	NM_001166158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase protein X component, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. Transcript Variant: This variant (3) lacks multiple in-frame exons in the central coding region, compared to variant 1, resulting in a protein (isoform 3) that lacks 227 aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.157379.1, AK301384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT 
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG 
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG 
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT 
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA 
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT 
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA 
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT 
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159630.1
CDS	527..1351
Translation	MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Target ORF information:

RefSeq Version	NM_001166158.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166158.1

ORF Insert Sequence:

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT 
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG 
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG 
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT 
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA 
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT 
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA 
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT 
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHX ( NM_003477.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003468.2 Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19013
Clone ID Related Accession (Same CDS sequence)	NM_003477.3 , NM_003477.2
Accession Version	NM_003477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_003477.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U82328.1, SRR1660803.16316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000227868.9/ ENSP00000227868.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003468.2
CDS	39..1544
Translation	MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Target ORF information:

RefSeq Version	NM_003477.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003477.3

ORF Insert Sequence:

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHX ( NM_003477.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003468.2 Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19013
Clone ID Related Accession (Same CDS sequence)	NM_003477.3 , NM_003477.2
Accession Version	NM_003477.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2008 this sequence version replaced NM_003477.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.266182.1, SRR1660805.33870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003468.2
CDS	527..2032
Translation	MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Target ORF information:

RefSeq Version	NM_003477.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003477.2

ORF Insert Sequence:

ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA 
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA 
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHX ( XM_011520390.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518692.1 Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.

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CloneID	OHu61608
Clone ID Related Accession (Same CDS sequence)	XM_011520390.1
Accession Version	XM_011520390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase protein X component, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518692.1
CDS	131..1456
Translation	MPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Target ORF information:

RefSeq Version	XM_011520390.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520390.1

ORF Insert Sequence:

ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT 
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA 
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT 
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC 
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA 
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC 
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC 
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT 
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC 
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC 
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT 
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC 
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT 
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT 
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT 
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA 
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG 
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG 
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA 
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC 
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHX ( NM_001135024.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128496.1 Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 2, mRNA.

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CloneID	OHu07230
Clone ID Related Accession (Same CDS sequence)	NM_001135024.1
Accession Version	NM_001135024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase protein X component, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA573433.1, BI915296.1, U79296.1 and AA282215.1. Transcript Variant: This variant (2) lacks a segment in the 5' region, resulting in upstream in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.78234.1, SRR1660803.174236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT 
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA 
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT 
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT 
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA 
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA 
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC 
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC 
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT 
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA 
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT 
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC 
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA 
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT 
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA 
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA 
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT 
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT 
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT 
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT 
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA 
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT 
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG 
AATCCTATCC GACTTGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128496.1
CDS	243..1703
Translation	MQSGGAEGSPGAGRTGRGPGSGKAPPAEISSGAPDFPGGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Target ORF information:

RefSeq Version	NM_001135024.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase complex component X (PDHX), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135024.1

ORF Insert Sequence:

ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT 
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA 
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT 
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT 
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA 
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA 
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC 
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC 
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT 
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA 
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT 
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC 
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA 
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT 
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA 
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA 
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT 
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT 
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT 
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT 
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA 
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT 
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG 
AATCCTATCC GACTTGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Cell159(7)1615-25(2014 Dec)
Mathias RA,Greco TM,Oberstein A,Budayeva HG,Chakrabarti R,Rowland EA,Kang Y,Shenk T,Cristea IM

Founder p.Arg 446* mutation in the PDHX gene explains over half of cases with congenital lactic acidosis in Roma children.
Molecular genetics and metabolism113(1-2)76-83(2014 Sep-Oct)
Ivanov IS,Azmanov DN,Ivanova MB,Chamova T,Pacheva IH,Panova MV,Song S,Morar B,Yordanova RV,Galabova FK,Sotkova IG,Linev AJ,Bitchev S,Shearwood AM,Kancheva D,Gabrikova D,Karcagi V,Guergueltcheva V,Geneva IE,Bozhinova V,Stoyanova VK,Kremensky I,Jordanova A,Savov A,Horvath R,Brown MA,Tournev I,Filipovska A,Kalaydjieva L

MicroRNA-26a regulates glucose metabolism by direct targeting PDHX in colorectal cancer cells.
BMC cancer14443(2014 Jun)
Chen B,Liu Y,Jin X,Lu W,Liu J,Xia Z,Yuan Q,Zhao X,Xu N,Liang S

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PDHX cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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