FLAD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FLAD1
Entrez Gene ID	80308
Full Name	flavin adenine dinucleotide synthetase 1
Synonyms	FAD1,FADS,LSMFLAD,PP591
General protein information	Preferred Names flavin adenine dinucleotide synthetase 1 Names FAD synthase FAD pyrophosphorylase FAD-synthetase FMN adenylyltransferase Fad1, flavin adenine dinucleotide synthetase, homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Summary	This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Disorder MIM:	610595
Disorder Html:	Lipid storage myopathy due to flavin adenine dinucleotide synthetase deficiency, 255100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184892.1	NP_001171821.1	FAD synthase isoform 4
NM_001184891.2	NP_001171820.1	FAD synthase isoform 3
NM_001184892.2	NP_001171821.1	FAD synthase isoform 4
NM_001184891.1	NP_001171820.1	FAD synthase isoform 3
NM_201398.3	NP_958800.1	FAD synthase isoform 2
NM_201398.2	NP_958800.1	FAD synthase isoform 2
NM_025207.4	NP_079483.3	FAD synthase isoform 1
NM_025207.5	NP_079483.3	FAD synthase isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Flad1	NP_796015.2
Gallus gallus (chicken)	FLAD1	XP_004948328.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002740	XP_312180.4
Saccharomyces cerevisiae (baker's yeast)	FAD1	NP_010239.1
Pan troglodytes (chimpanzee)	FLAD1	XP_003308466.1
Rattus norvegicus (Norway rat)	Flad1	NP_001103608.1
Danio rerio (zebrafish)	flad1	NP_001003997.1
Canis lupus familiaris (dog)	FLAD1	XP_855063.1
Bos taurus (cattle)	FLAD1	NP_001030211.2
Drosophila melanogaster (fruit fly)	CG4407	NP_572837.1
Schizosaccharomyces pombe (fission yeast)	SPCC1235.04c	NP_587730.1
	FLAD1	XP_001114732.1
Caenorhabditis elegans (roundworm)	flad-1	NP_001022287.1
Kluyveromyces lactis	KLLA0E13377g	XP_454552.1
Magnaporthe oryzae (rice blast fungus)	MGG_12267	XP_003715001.1
Homo sapiens (human)	FLAD1	NP_079483.3
Eremothecium gossypii	AGOS_AER198W	NP_985055.1
Neurospora crassa	NCU09233	XP_963810.2

Gene Ontology
Bibliography

Related articles in PubMed

Riboflavin-Responsive and -Non-responsive Mutations in FAD Synthase Cause Multiple Acyl-CoA Dehydrogenase and Combined Respiratory-Chain Deficiency.
Olsen RKJ, Ko?a??kov? E, Giancaspero TA, Mosegaard S, Boczonadi V, Matakovi? L, Veauville-Merlli? A, Terrile C, Schwarzmayr T, Haack TB, Auranen M, Leone P, Galluccio M, Imbard A, Gutierrez-Rios P, Palmfeldt J, Graf E, Vianey-Saban C, Oppenheim M, Schiff M, Pichard S, Rigal O, Pyle A, Chinnery PF, Konstantopoulou V, M?slinger D, Feichtinger RG, Talim B, Topaloglu H, Coskun T, Gucer S, Botta A, Pegoraro E, Malena A, Vergani L, Mazz? D, Zollino M, Ghezzi D, Acquaviva C, Tyni T, Boneh A, Meitinger T, Strom TM, Gregersen N, Mayr JA, Horvath R, Barile M, Prokisch H
American journal of human genetics98(6)1130-1145(2016 Jun)

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Eeles RA, Olama AA, Benlloch S, Saunders EJ, Leongamornlert DA, Tymrakiewicz M, Ghoussaini M, Luccarini C, Dennis J, Jugurnauth-Little S, Dadaev T, Neal DE, Hamdy FC, Donovan JL, Muir K, Giles GG, Severi G, Wiklund F, Gronberg H, Haiman CA, Schumacher F, Henderson BE, Le Marchand L, Lindstrom S, Kraft P, Hunter DJ, Gapstur S, Chanock SJ, Berndt SI, Albanes D, Andriole G, Schleutker J, Weischer M, Canzian F, Riboli E, Key TJ, Travis RC, Campa D, Ingles SA, John EM, Hayes RB, Pharoah PD, Pashayan N, Khaw KT, Stanford JL, Ostrander EA, Signorello LB, Thibodeau SN, Schaid D, Maier C, Vogel W, Kibel AS, Cybulski C, Lubinski J, Cannon-Albright L, Brenner H, Park JY, Kaneva R, Batra J, Spurdle AB, Clements JA, Teixeira MR, Dicks E, Lee A, Dunning AM, Baynes C, Conroy D, Maranian MJ, Ahmed S, Govindasami K, Guy M, Wilkinson RA, Sawyer EJ, Morgan A, Dearnaley DP, Horwich A, Huddart RA, Khoo VS, Parker CC, Van As NJ, Woodhouse CJ, Thompson A, Dudderidge T, Ogden C, Cooper CS, Lophatananon A, Cox A, Southey MC, Hopper JL, English DR, Aly M, Adolfsson J, Xu J, Zheng SL, Yeager M, Kaaks R, Diver WR, Gaudet MM, Stern MC, Corral R, Joshi AD, Shahabi A, Wahlfors T, Tammela TL, Auvinen A, Virtamo J, Klarskov P, Nordestgaard BG, R?der MA, Nielsen SF, Bojesen SE, Siddiq A, Fitzgerald LM, Kolb S, Kwon EM, Karyadi DM, Blot WJ, Zheng W, Cai Q, McDonnell SK, Rinckleb AE, Drake B, Colditz G, Wokolorczyk D, Stephenson RA, Teerlink C, Muller H, Rothenbacher D, Sellers TA, Lin HY, Slavov C, Mitev V, Lose F, Srinivasan S, Maia S, Paulo P, Lange E, Cooney KA, Antoniou AC, Vincent D, Bacot F, Tessier DC, , , , , , Kote-Jarai Z, Easton DF
Nature genetics45(4)385-91, 391e1-2(2013 Apr)

Specific electron transport chain abnormalities in amyotrophic lateral sclerosis.
Lin J, Diamanduros A, Chowdhury SA, Scelsa S, Latov N, Sadiq SA
Journal of neurology256(5)774-82(2009 May)

Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase.
Brizio C, Galluccio M, Wait R, Torchetti EM, Bafunno V, Accardi R, Gianazza E, Indiveri C, Barile M
Biochemical and biophysical research communications344(3)1008-16(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Frameshift mutation in the FADS gene is Associated with Multiple Acyl-CoA Dehydrogenase and Combined Respiratory-Chain Deficiency.
Title: Riboflavin-Responsive and -Non-responsive Mutations in FAD Synthase Cause Multiple Acyl-CoA Dehydrogenase and Combined Respiratory-Chain Deficiency.

Our data show that a reduction in electron transport proteins, namely FAD synthetase, RFK, CYC1, and SDHB, is seen in patients with ALS.
Title: Specific electron transport chain abnormalities in amyotrophic lateral sclerosis.

The following FLAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13970	NM_201398.3 Latest version!	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13989	NM_025207.5 Latest version!	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu13904	NM_001184892.2 Latest version!	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu13971	NM_001184891.2 Latest version!	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu13904	NM_001184892.1	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu13971	NM_001184891.1	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu13970	NM_201398.2	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13989	NM_025207.4	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLAD1 ( NM_201398.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_958800.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13970
Clone ID Related Accession (Same CDS sequence)	NM_201398.3 , NM_201398.2
Accession Version	NM_201398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	FAD synthase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and BC021096.1. On May 31, 2019 this sequence version replaced NM_201398.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.172101.1, BC032323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGATA CACATCACTG GGGAGTCGGG AGAATACCGT GCGGAACCCG 
GCCCTGAAGT GCCTGAGCCC AGGAGGACAC CCCACATACC GTCCAGCCTA TCTACTGGAG 
AACGAAGAAG AGGAGCGGAA CTCCCGCACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958800.1
CDS	199..1671
Translation	MTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Target ORF information:

RefSeq Version	NM_201398.3
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201398.3

ORF Insert Sequence:

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGATA CACATCACTG GGGAGTCGGG AGAATACCGT GCGGAACCCG 
GCCCTGAAGT GCCTGAGCCC AGGAGGACAC CCCACATACC GTCCAGCCTA TCTACTGGAG 
AACGAAGAAG AGGAGCGGAA CTCCCGCACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_201398.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_958800.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13970
Clone ID Related Accession (Same CDS sequence)	NM_201398.3 , NM_201398.2
Accession Version	NM_201398.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	FAD synthase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and BC021096.1. On May 21, 2010 this sequence version replaced NM_201398.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC032323.1, SRR1163655.474089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGATA CACATCACTG GGGAGTCGGG AGAATACCGT GCGGAACCCG 
GCCCTGAAGT GCCTGAGCCC AGGAGGACAC CCCACATACC GTCCAGCCTA TCTACTGGAG 
AACGAAGAAG AGGAGCGGAA CTCCCGCACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958800.1
CDS	249..1721
Translation	MTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Target ORF information:

RefSeq Version	NM_201398.2
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201398.2

ORF Insert Sequence:

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGATA CACATCACTG GGGAGTCGGG AGAATACCGT GCGGAACCCG 
GCCCTGAAGT GCCTGAGCCC AGGAGGACAC CCCACATACC GTCCAGCCTA TCTACTGGAG 
AACGAAGAAG AGGAGCGGAA CTCCCGCACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_025207.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079483.3 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.

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CloneID	OHu13989
Clone ID Related Accession (Same CDS sequence)	NM_025207.4 , NM_025207.5
Accession Version	NM_025207.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	FAD synthase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and AF520568.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2010 this sequence version replaced NM_025207.3. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AF520568.1, SRR1803613.157146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTGGG ATTTGGGAAC ACGTTTATTC CAGAGGCAGG AACAAAGGAG TCGCTTGTCA 
AGGATCTGGT TAGAGAAGAC TAGGGTCTTC CTCGAAGGAA GCACGCGCAC GCCTGCTCTC 
CCCCATTGTC TTTTCTGGCT TCTCCAGGTT CCCTCGACCC AGGACCCCCT GTTCCCAGGC 
TATGGCCCCC AGTGCCCTGT AGACCTGGCA GGCCCCCCGT GCTTGCGACC CCTATTTGGG 
GGTCTGGGTG GCTACTGGAG GGCCTTGCAG AGGGGCAGAG AAGGCAGGAC CATGACATCT 
AGGGCCTCTG AACTTTCTCC GGGGCGCAGC GTGACGGCTG GCATCATCAT TGTTGGAGAT 
GAGATCCTTA AGGGACACAC TCAGGACACC AACACCTTCT TTCTGTGCCG GACACTGCGC 
TCCCTAGGGG TCCAGGTTTG CCGAGTCTCA GTTGTACCTG ATGAGGTAGC CACCATTGCA 
GCTGAGGTCA CTTCTTTCTC CAACCGCTTC ACCCATGTCC TCACAGCAGG GGGCATCGGC 
CCCACTCATG ATGATGTGAC CTTTGAGGCA GTGGCACAGG CCTTTGGAGA TGAGCTGAAG 
CCACACCCCA AGTTGGAAGC AGCCACCAAA GCCCTAGGAG GGGAAGGCTG GGAGAAGCTA 
TCATTGGTGC CCTCCTCTGC CCGCCTGCAT TATGGCACAG ATCCTTGCAC TGGTCAACCT 
TTCAGATTCC CTCTGGTCTC CGTCCGAAAC GTCTACCTCT TCCCAGGCAT TCCAGAGCTG 
CTGCGGCGGG TGCTGGAGGG GATGAAGGGA CTATTCCAAA ACCCAGCTGT TCAGTTCCAC 
TCAAAGGAGC TATATGTGGC TGCTGATGAA GCCTCCATCG CCCCCATTCT GGCTGAGGCC 
CAGGCCCACT TTGGACGTAG GCTTGGCCTG GGTTCCTACC CTGACTGGGG CAGCAACTAC 
TATCAGGTGA AGCTGACTCT AGACTCAGAG GAAGAAGGAC CCCTGGAGGA ATGCTTGGCC 
TACCTGACTG CCCGTTTGCC CCAGGGATCG CTGGTCCCCT ACATGCCCAA CGCTGTGGAG 
CAGGCCAGTG AGGCTGTATA CAAACTCGCT GAATCAGGGT CTTCTTTGGG GAAAAAGGTG 
GCAGGTGCCC TACAGACCAT TGAGACCTCC CTGGCTCAGT ACAGCCTCAC CCAGCTCTGT 
GTGGGCTTCA ACGGGGGCAA AGACTGCACT GCCCTCCTGC ACCTCTTCCA TGCAGCTGTG 
CAGAGGAAAT TACCTGATGT TCCAAACCCC CTCCAGATCC TGTATATCCG CAGCATCTCC 
CCTTTCCCTG AGCTGGAACA GTTTCTACAG GACACTATCA AGAGGTATAA TCTGCAGATG 
TTGGAAGCTG AGGGCAGCAT GAAGCAGGCC CTGGGTGAAC TGCAGGCACG GCACCCCCAG 
CTGGAGGCTG TCCTTATGGG CACCCGCCGG ACTGACCCCT ACTCCTGTAG CCTCTGCCCT 
TTCAGCCCCA CTGACCCAGG CTGGCCCGCA TTCATGCGCA TCAACCCACT GCTGGACTGG 
ACCTACAGAG ACATCTGGGA TTTTCTGCGT CAGCTGTTTG TCCCATACTG TATCCTGTAT 
GACCGAGGAT ACACATCACT GGGGAGTCGG GAGAATACCG TGCGGAACCC GGCCCTGAAG 
TGCCTGAGCC CAGGAGGACA CCCCACATAC CGTCCAGCCT ATCTACTGGA GAACGAAGAA 
GAGGAGCGGA ACTCCCGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079483.3
CDS	402..2165
Translation	MGWDLGTRLFQRQEQRSRLSRIWLEKTRVFLEGSTRTPALPHCLFWLLQVPSTQDPLFPGYGPQCPVDLAGPPCLRPLFGGLGGYWRALQRGREGRTMTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Target ORF information:

RefSeq Version	NM_025207.4
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025207.4

ORF Insert Sequence:

ATGGGTTGGG ATTTGGGAAC ACGTTTATTC CAGAGGCAGG AACAAAGGAG TCGCTTGTCA 
AGGATCTGGT TAGAGAAGAC TAGGGTCTTC CTCGAAGGAA GCACGCGCAC GCCTGCTCTC 
CCCCATTGTC TTTTCTGGCT TCTCCAGGTT CCCTCGACCC AGGACCCCCT GTTCCCAGGC 
TATGGCCCCC AGTGCCCTGT AGACCTGGCA GGCCCCCCGT GCTTGCGACC CCTATTTGGG 
GGTCTGGGTG GCTACTGGAG GGCCTTGCAG AGGGGCAGAG AAGGCAGGAC CATGACATCT 
AGGGCCTCTG AACTTTCTCC GGGGCGCAGC GTGACGGCTG GCATCATCAT TGTTGGAGAT 
GAGATCCTTA AGGGACACAC TCAGGACACC AACACCTTCT TTCTGTGCCG GACACTGCGC 
TCCCTAGGGG TCCAGGTTTG CCGAGTCTCA GTTGTACCTG ATGAGGTAGC CACCATTGCA 
GCTGAGGTCA CTTCTTTCTC CAACCGCTTC ACCCATGTCC TCACAGCAGG GGGCATCGGC 
CCCACTCATG ATGATGTGAC CTTTGAGGCA GTGGCACAGG CCTTTGGAGA TGAGCTGAAG 
CCACACCCCA AGTTGGAAGC AGCCACCAAA GCCCTAGGAG GGGAAGGCTG GGAGAAGCTA 
TCATTGGTGC CCTCCTCTGC CCGCCTGCAT TATGGCACAG ATCCTTGCAC TGGTCAACCT 
TTCAGATTCC CTCTGGTCTC CGTCCGAAAC GTCTACCTCT TCCCAGGCAT TCCAGAGCTG 
CTGCGGCGGG TGCTGGAGGG GATGAAGGGA CTATTCCAAA ACCCAGCTGT TCAGTTCCAC 
TCAAAGGAGC TATATGTGGC TGCTGATGAA GCCTCCATCG CCCCCATTCT GGCTGAGGCC 
CAGGCCCACT TTGGACGTAG GCTTGGCCTG GGTTCCTACC CTGACTGGGG CAGCAACTAC 
TATCAGGTGA AGCTGACTCT AGACTCAGAG GAAGAAGGAC CCCTGGAGGA ATGCTTGGCC 
TACCTGACTG CCCGTTTGCC CCAGGGATCG CTGGTCCCCT ACATGCCCAA CGCTGTGGAG 
CAGGCCAGTG AGGCTGTATA CAAACTCGCT GAATCAGGGT CTTCTTTGGG GAAAAAGGTG 
GCAGGTGCCC TACAGACCAT TGAGACCTCC CTGGCTCAGT ACAGCCTCAC CCAGCTCTGT 
GTGGGCTTCA ACGGGGGCAA AGACTGCACT GCCCTCCTGC ACCTCTTCCA TGCAGCTGTG 
CAGAGGAAAT TACCTGATGT TCCAAACCCC CTCCAGATCC TGTATATCCG CAGCATCTCC 
CCTTTCCCTG AGCTGGAACA GTTTCTACAG GACACTATCA AGAGGTATAA TCTGCAGATG 
TTGGAAGCTG AGGGCAGCAT GAAGCAGGCC CTGGGTGAAC TGCAGGCACG GCACCCCCAG 
CTGGAGGCTG TCCTTATGGG CACCCGCCGG ACTGACCCCT ACTCCTGTAG CCTCTGCCCT 
TTCAGCCCCA CTGACCCAGG CTGGCCCGCA TTCATGCGCA TCAACCCACT GCTGGACTGG 
ACCTACAGAG ACATCTGGGA TTTTCTGCGT CAGCTGTTTG TCCCATACTG TATCCTGTAT 
GACCGAGGAT ACACATCACT GGGGAGTCGG GAGAATACCG TGCGGAACCC GGCCCTGAAG 
TGCCTGAGCC CAGGAGGACA CCCCACATAC CGTCCAGCCT ATCTACTGGA GAACGAAGAA 
GAGGAGCGGA ACTCCCGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_025207.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_079483.3 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.

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CloneID	OHu13989
Clone ID Related Accession (Same CDS sequence)	NM_025207.4 , NM_025207.5
Accession Version	NM_025207.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	FAD synthase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and AF520568.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_025207.4. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AF520568.1, SRR1803613.157146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000292180.8/ ENSP00000292180.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTTGGG ATTTGGGAAC ACGTTTATTC CAGAGGCAGG AACAAAGGAG TCGCTTGTCA 
AGGATCTGGT TAGAGAAGAC TAGGGTCTTC CTCGAAGGAA GCACGCGCAC GCCTGCTCTC 
CCCCATTGTC TTTTCTGGCT TCTCCAGGTT CCCTCGACCC AGGACCCCCT GTTCCCAGGC 
TATGGCCCCC AGTGCCCTGT AGACCTGGCA GGCCCCCCGT GCTTGCGACC CCTATTTGGG 
GGTCTGGGTG GCTACTGGAG GGCCTTGCAG AGGGGCAGAG AAGGCAGGAC CATGACATCT 
AGGGCCTCTG AACTTTCTCC GGGGCGCAGC GTGACGGCTG GCATCATCAT TGTTGGAGAT 
GAGATCCTTA AGGGACACAC TCAGGACACC AACACCTTCT TTCTGTGCCG GACACTGCGC 
TCCCTAGGGG TCCAGGTTTG CCGAGTCTCA GTTGTACCTG ATGAGGTAGC CACCATTGCA 
GCTGAGGTCA CTTCTTTCTC CAACCGCTTC ACCCATGTCC TCACAGCAGG GGGCATCGGC 
CCCACTCATG ATGATGTGAC CTTTGAGGCA GTGGCACAGG CCTTTGGAGA TGAGCTGAAG 
CCACACCCCA AGTTGGAAGC AGCCACCAAA GCCCTAGGAG GGGAAGGCTG GGAGAAGCTA 
TCATTGGTGC CCTCCTCTGC CCGCCTGCAT TATGGCACAG ATCCTTGCAC TGGTCAACCT 
TTCAGATTCC CTCTGGTCTC CGTCCGAAAC GTCTACCTCT TCCCAGGCAT TCCAGAGCTG 
CTGCGGCGGG TGCTGGAGGG GATGAAGGGA CTATTCCAAA ACCCAGCTGT TCAGTTCCAC 
TCAAAGGAGC TATATGTGGC TGCTGATGAA GCCTCCATCG CCCCCATTCT GGCTGAGGCC 
CAGGCCCACT TTGGACGTAG GCTTGGCCTG GGTTCCTACC CTGACTGGGG CAGCAACTAC 
TATCAGGTGA AGCTGACTCT AGACTCAGAG GAAGAAGGAC CCCTGGAGGA ATGCTTGGCC 
TACCTGACTG CCCGTTTGCC CCAGGGATCG CTGGTCCCCT ACATGCCCAA CGCTGTGGAG 
CAGGCCAGTG AGGCTGTATA CAAACTCGCT GAATCAGGGT CTTCTTTGGG GAAAAAGGTG 
GCAGGTGCCC TACAGACCAT TGAGACCTCC CTGGCTCAGT ACAGCCTCAC CCAGCTCTGT 
GTGGGCTTCA ACGGGGGCAA AGACTGCACT GCCCTCCTGC ACCTCTTCCA TGCAGCTGTG 
CAGAGGAAAT TACCTGATGT TCCAAACCCC CTCCAGATCC TGTATATCCG CAGCATCTCC 
CCTTTCCCTG AGCTGGAACA GTTTCTACAG GACACTATCA AGAGGTATAA TCTGCAGATG 
TTGGAAGCTG AGGGCAGCAT GAAGCAGGCC CTGGGTGAAC TGCAGGCACG GCACCCCCAG 
CTGGAGGCTG TCCTTATGGG CACCCGCCGG ACTGACCCCT ACTCCTGTAG CCTCTGCCCT 
TTCAGCCCCA CTGACCCAGG CTGGCCCGCA TTCATGCGCA TCAACCCACT GCTGGACTGG 
ACCTACAGAG ACATCTGGGA TTTTCTGCGT CAGCTGTTTG TCCCATACTG TATCCTGTAT 
GACCGAGGAT ACACATCACT GGGGAGTCGG GAGAATACCG TGCGGAACCC GGCCCTGAAG 
TGCCTGAGCC CAGGAGGACA CCCCACATAC CGTCCAGCCT ATCTACTGGA GAACGAAGAA 
GAGGAGCGGA ACTCCCGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079483.3
CDS	352..2115
Translation	MGWDLGTRLFQRQEQRSRLSRIWLEKTRVFLEGSTRTPALPHCLFWLLQVPSTQDPLFPGYGPQCPVDLAGPPCLRPLFGGLGGYWRALQRGREGRTMTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Target ORF information:

RefSeq Version	NM_025207.5
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025207.5

ORF Insert Sequence:

ATGGGTTGGG ATTTGGGAAC ACGTTTATTC CAGAGGCAGG AACAAAGGAG TCGCTTGTCA 
AGGATCTGGT TAGAGAAGAC TAGGGTCTTC CTCGAAGGAA GCACGCGCAC GCCTGCTCTC 
CCCCATTGTC TTTTCTGGCT TCTCCAGGTT CCCTCGACCC AGGACCCCCT GTTCCCAGGC 
TATGGCCCCC AGTGCCCTGT AGACCTGGCA GGCCCCCCGT GCTTGCGACC CCTATTTGGG 
GGTCTGGGTG GCTACTGGAG GGCCTTGCAG AGGGGCAGAG AAGGCAGGAC CATGACATCT 
AGGGCCTCTG AACTTTCTCC GGGGCGCAGC GTGACGGCTG GCATCATCAT TGTTGGAGAT 
GAGATCCTTA AGGGACACAC TCAGGACACC AACACCTTCT TTCTGTGCCG GACACTGCGC 
TCCCTAGGGG TCCAGGTTTG CCGAGTCTCA GTTGTACCTG ATGAGGTAGC CACCATTGCA 
GCTGAGGTCA CTTCTTTCTC CAACCGCTTC ACCCATGTCC TCACAGCAGG GGGCATCGGC 
CCCACTCATG ATGATGTGAC CTTTGAGGCA GTGGCACAGG CCTTTGGAGA TGAGCTGAAG 
CCACACCCCA AGTTGGAAGC AGCCACCAAA GCCCTAGGAG GGGAAGGCTG GGAGAAGCTA 
TCATTGGTGC CCTCCTCTGC CCGCCTGCAT TATGGCACAG ATCCTTGCAC TGGTCAACCT 
TTCAGATTCC CTCTGGTCTC CGTCCGAAAC GTCTACCTCT TCCCAGGCAT TCCAGAGCTG 
CTGCGGCGGG TGCTGGAGGG GATGAAGGGA CTATTCCAAA ACCCAGCTGT TCAGTTCCAC 
TCAAAGGAGC TATATGTGGC TGCTGATGAA GCCTCCATCG CCCCCATTCT GGCTGAGGCC 
CAGGCCCACT TTGGACGTAG GCTTGGCCTG GGTTCCTACC CTGACTGGGG CAGCAACTAC 
TATCAGGTGA AGCTGACTCT AGACTCAGAG GAAGAAGGAC CCCTGGAGGA ATGCTTGGCC 
TACCTGACTG CCCGTTTGCC CCAGGGATCG CTGGTCCCCT ACATGCCCAA CGCTGTGGAG 
CAGGCCAGTG AGGCTGTATA CAAACTCGCT GAATCAGGGT CTTCTTTGGG GAAAAAGGTG 
GCAGGTGCCC TACAGACCAT TGAGACCTCC CTGGCTCAGT ACAGCCTCAC CCAGCTCTGT 
GTGGGCTTCA ACGGGGGCAA AGACTGCACT GCCCTCCTGC ACCTCTTCCA TGCAGCTGTG 
CAGAGGAAAT TACCTGATGT TCCAAACCCC CTCCAGATCC TGTATATCCG CAGCATCTCC 
CCTTTCCCTG AGCTGGAACA GTTTCTACAG GACACTATCA AGAGGTATAA TCTGCAGATG 
TTGGAAGCTG AGGGCAGCAT GAAGCAGGCC CTGGGTGAAC TGCAGGCACG GCACCCCCAG 
CTGGAGGCTG TCCTTATGGG CACCCGCCGG ACTGACCCCT ACTCCTGTAG CCTCTGCCCT 
TTCAGCCCCA CTGACCCAGG CTGGCCCGCA TTCATGCGCA TCAACCCACT GCTGGACTGG 
ACCTACAGAG ACATCTGGGA TTTTCTGCGT CAGCTGTTTG TCCCATACTG TATCCTGTAT 
GACCGAGGAT ACACATCACT GGGGAGTCGG GAGAATACCG TGCGGAACCC GGCCCTGAAG 
TGCCTGAGCC CAGGAGGACA CCCCACATAC CGTCCAGCCT ATCTACTGGA GAACGAAGAA 
GAGGAGCGGA ACTCCCGCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_001184892.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171821.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.

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CloneID	OHu13904
Clone ID Related Accession (Same CDS sequence)	NM_001184892.1 , NM_001184892.2
Accession Version	NM_001184892.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	FAD synthase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and AF218022.1. Transcript Variant: This variant (4) has multiple differences and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 4) has a distinct N- and C-termini and is shorter when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF218022.1, SRR1660803.310878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCAT CATCTTCAAC CCCTCCCCTT CATCCCTACA GTACTGATGG CCTCATCTTC 
CCCTTCAACC CCCAGGGACA CACTCAGGAC ACCAACACCT TCTTTCTGTG CCGGACACTG 
CGCTCCCTAG GGGTCCAGGT TTGCCGAGTC TCAGTTGTAC CTGATGAGGT AGCCACCATT 
GCAGCTGAGG TCACTTCTTT CTCCAACCGC TTCACCCATG TCCTCACAGC AGGGGGCATC 
GGCCCCACTC ATGATGATGT GACCTTTGAG GCAGTGGCAC AGGCCTTTGG AGATGAGCTG 
AAGCCACACC CCAAGTTGGA AGCAGCCACC AAAGCCCTAG GAGGGGAAGG CTGGGAGAAG 
CTATCATTGG TGCCCTCCTC TGCCCGCCTG CATTATGGCA CAGATCCTTG CACTGGTCAA 
CCTTTCAGAT TCCCTCTGGT CTCCGTCCGA AACGTCTACC TCTTCCCAGG CATTCCAGAG 
CTGCTGCGGC GGGTGCTGGA GGGGATGAAG GGACTATTCC AAAACCCAGC TGTTCAGTTC 
CACTCAAAGG AGCTATATGT GGCTGCTGAT GAAGCCTCCA TCGCCCCCAT TCTGGCTGAG 
GCCCAGGCCC ACTTTGGACG TAGGCTTGGC CTGGGTTCCT ACCCTGACTG GGGCAGCAAC 
TACTATCAGG TGAAGCTGAC TCTAGACTCA GAGGAAGAAG GACCCCTGGA GGAATGCTTG 
GCCTACCTGA CTGCCCGTTT GCCCCAGGGA TCGCTGGTCC CCTACATGCC CAACGCTGTG 
GAGCAGGCCA GTGAGGCTGT ATACAAACTC GCTGAATCAG GTAGGGACCT TATGGAGGAG 
GGGCATTATG CCCAAAGCCA TTGGTGGCAC CCCAGATCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171821.1
CDS	894..1778
Translation	MQPSSSTPPLHPYSTDGLIFPFNPQGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGRDLMEEGHYAQSHWWHPRSQ

Target ORF information:

RefSeq Version	NM_001184892.1
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184892.1

ORF Insert Sequence:

ATGCAGCCAT CATCTTCAAC CCCTCCCCTT CATCCCTACA GTACTGATGG CCTCATCTTC 
CCCTTCAACC CCCAGGGACA CACTCAGGAC ACCAACACCT TCTTTCTGTG CCGGACACTG 
CGCTCCCTAG GGGTCCAGGT TTGCCGAGTC TCAGTTGTAC CTGATGAGGT AGCCACCATT 
GCAGCTGAGG TCACTTCTTT CTCCAACCGC TTCACCCATG TCCTCACAGC AGGGGGCATC 
GGCCCCACTC ATGATGATGT GACCTTTGAG GCAGTGGCAC AGGCCTTTGG AGATGAGCTG 
AAGCCACACC CCAAGTTGGA AGCAGCCACC AAAGCCCTAG GAGGGGAAGG CTGGGAGAAG 
CTATCATTGG TGCCCTCCTC TGCCCGCCTG CATTATGGCA CAGATCCTTG CACTGGTCAA 
CCTTTCAGAT TCCCTCTGGT CTCCGTCCGA AACGTCTACC TCTTCCCAGG CATTCCAGAG 
CTGCTGCGGC GGGTGCTGGA GGGGATGAAG GGACTATTCC AAAACCCAGC TGTTCAGTTC 
CACTCAAAGG AGCTATATGT GGCTGCTGAT GAAGCCTCCA TCGCCCCCAT TCTGGCTGAG 
GCCCAGGCCC ACTTTGGACG TAGGCTTGGC CTGGGTTCCT ACCCTGACTG GGGCAGCAAC 
TACTATCAGG TGAAGCTGAC TCTAGACTCA GAGGAAGAAG GACCCCTGGA GGAATGCTTG 
GCCTACCTGA CTGCCCGTTT GCCCCAGGGA TCGCTGGTCC CCTACATGCC CAACGCTGTG 
GAGCAGGCCA GTGAGGCTGT ATACAAACTC GCTGAATCAG GTAGGGACCT TATGGAGGAG 
GGGCATTATG CCCAAAGCCA TTGGTGGCAC CCCAGATCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_001184892.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171821.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.

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CloneID	OHu13904
Clone ID Related Accession (Same CDS sequence)	NM_001184892.1 , NM_001184892.2
Accession Version	NM_001184892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	FAD synthase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1 and AF218022.1. On May 31, 2019 this sequence version replaced NM_001184892.1. Transcript Variant: This variant (4) has multiple differences and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 4) has a distinct N- and C-termini and is shorter when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF218022.1, SRR1660803.310878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCAT CATCTTCAAC CCCTCCCCTT CATCCCTACA GTACTGATGG CCTCATCTTC 
CCCTTCAACC CCCAGGGACA CACTCAGGAC ACCAACACCT TCTTTCTGTG CCGGACACTG 
CGCTCCCTAG GGGTCCAGGT TTGCCGAGTC TCAGTTGTAC CTGATGAGGT AGCCACCATT 
GCAGCTGAGG TCACTTCTTT CTCCAACCGC TTCACCCATG TCCTCACAGC AGGGGGCATC 
GGCCCCACTC ATGATGATGT GACCTTTGAG GCAGTGGCAC AGGCCTTTGG AGATGAGCTG 
AAGCCACACC CCAAGTTGGA AGCAGCCACC AAAGCCCTAG GAGGGGAAGG CTGGGAGAAG 
CTATCATTGG TGCCCTCCTC TGCCCGCCTG CATTATGGCA CAGATCCTTG CACTGGTCAA 
CCTTTCAGAT TCCCTCTGGT CTCCGTCCGA AACGTCTACC TCTTCCCAGG CATTCCAGAG 
CTGCTGCGGC GGGTGCTGGA GGGGATGAAG GGACTATTCC AAAACCCAGC TGTTCAGTTC 
CACTCAAAGG AGCTATATGT GGCTGCTGAT GAAGCCTCCA TCGCCCCCAT TCTGGCTGAG 
GCCCAGGCCC ACTTTGGACG TAGGCTTGGC CTGGGTTCCT ACCCTGACTG GGGCAGCAAC 
TACTATCAGG TGAAGCTGAC TCTAGACTCA GAGGAAGAAG GACCCCTGGA GGAATGCTTG 
GCCTACCTGA CTGCCCGTTT GCCCCAGGGA TCGCTGGTCC CCTACATGCC CAACGCTGTG 
GAGCAGGCCA GTGAGGCTGT ATACAAACTC GCTGAATCAG GTAGGGACCT TATGGAGGAG 
GGGCATTATG CCCAAAGCCA TTGGTGGCAC CCCAGATCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171821.1
CDS	844..1728
Translation	MQPSSSTPPLHPYSTDGLIFPFNPQGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGRDLMEEGHYAQSHWWHPRSQ

Target ORF information:

RefSeq Version	NM_001184892.2
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184892.2

ORF Insert Sequence:

ATGCAGCCAT CATCTTCAAC CCCTCCCCTT CATCCCTACA GTACTGATGG CCTCATCTTC 
CCCTTCAACC CCCAGGGACA CACTCAGGAC ACCAACACCT TCTTTCTGTG CCGGACACTG 
CGCTCCCTAG GGGTCCAGGT TTGCCGAGTC TCAGTTGTAC CTGATGAGGT AGCCACCATT 
GCAGCTGAGG TCACTTCTTT CTCCAACCGC TTCACCCATG TCCTCACAGC AGGGGGCATC 
GGCCCCACTC ATGATGATGT GACCTTTGAG GCAGTGGCAC AGGCCTTTGG AGATGAGCTG 
AAGCCACACC CCAAGTTGGA AGCAGCCACC AAAGCCCTAG GAGGGGAAGG CTGGGAGAAG 
CTATCATTGG TGCCCTCCTC TGCCCGCCTG CATTATGGCA CAGATCCTTG CACTGGTCAA 
CCTTTCAGAT TCCCTCTGGT CTCCGTCCGA AACGTCTACC TCTTCCCAGG CATTCCAGAG 
CTGCTGCGGC GGGTGCTGGA GGGGATGAAG GGACTATTCC AAAACCCAGC TGTTCAGTTC 
CACTCAAAGG AGCTATATGT GGCTGCTGAT GAAGCCTCCA TCGCCCCCAT TCTGGCTGAG 
GCCCAGGCCC ACTTTGGACG TAGGCTTGGC CTGGGTTCCT ACCCTGACTG GGGCAGCAAC 
TACTATCAGG TGAAGCTGAC TCTAGACTCA GAGGAAGAAG GACCCCTGGA GGAATGCTTG 
GCCTACCTGA CTGCCCGTTT GCCCCAGGGA TCGCTGGTCC CCTACATGCC CAACGCTGTG 
GAGCAGGCCA GTGAGGCTGT ATACAAACTC GCTGAATCAG GTAGGGACCT TATGGAGGAG 
GGGCATTATG CCCAAAGCCA TTGGTGGCAC CCCAGATCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_001184891.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171820.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13971
Clone ID Related Accession (Same CDS sequence)	NM_001184891.2 , NM_001184891.1
Accession Version	NM_001184891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	FAD synthase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1, BC011378.1 and AF520568.1. On Jun 1, 2019 this sequence version replaced NM_001184891.1. Transcript Variant: This variant (3) differs in its 5' and 3' ends and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus and a distinct and shorter C-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC011378.1, SRR1803611.255889.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171820.1
CDS	199..1539
Translation	MTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRG

Target ORF information:

RefSeq Version	NM_001184891.2
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184891.2

ORF Insert Sequence:

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLAD1 ( NM_001184891.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171820.1 Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13971
Clone ID Related Accession (Same CDS sequence)	NM_001184891.2 , NM_001184891.1
Accession Version	NM_001184891.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	FAD synthase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC371856.1, BC011378.1 and AF520568.1. Transcript Variant: This variant (3) differs in its 5' and 3' ends and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus and a distinct and shorter C-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC011378.1, SRR1803611.255889.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171820.1
CDS	249..1589
Translation	MTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRG

Target ORF information:

RefSeq Version	NM_001184891.1
Organism	Homo sapiens(human)
Definition	Homo sapiens flavin adenine dinucleotide synthetase 1 (FLAD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184891.1

ORF Insert Sequence:

ATGACATCTA GGGCCTCTGA ACTTTCTCCG GGGCGCAGCG TGACGGCTGG CATCATCATT 
GTTGGAGATG AGATCCTTAA GGGACACACT CAGGACACCA ACACCTTCTT TCTGTGCCGG 
ACACTGCGCT CCCTAGGGGT CCAGGTTTGC CGAGTCTCAG TTGTACCTGA TGAGGTAGCC 
ACCATTGCAG CTGAGGTCAC TTCTTTCTCC AACCGCTTCA CCCATGTCCT CACAGCAGGG 
GGCATCGGCC CCACTCATGA TGATGTGACC TTTGAGGCAG TGGCACAGGC CTTTGGAGAT 
GAGCTGAAGC CACACCCCAA GTTGGAAGCA GCCACCAAAG CCCTAGGAGG GGAAGGCTGG 
GAGAAGCTAT CATTGGTGCC CTCCTCTGCC CGCCTGCATT ATGGCACAGA TCCTTGCACT 
GGTCAACCTT TCAGATTCCC TCTGGTCTCC GTCCGAAACG TCTACCTCTT CCCAGGCATT 
CCAGAGCTGC TGCGGCGGGT GCTGGAGGGG ATGAAGGGAC TATTCCAAAA CCCAGCTGTT 
CAGTTCCACT CAAAGGAGCT ATATGTGGCT GCTGATGAAG CCTCCATCGC CCCCATTCTG 
GCTGAGGCCC AGGCCCACTT TGGACGTAGG CTTGGCCTGG GTTCCTACCC TGACTGGGGC 
AGCAACTACT ATCAGGTGAA GCTGACTCTA GACTCAGAGG AAGAAGGACC CCTGGAGGAA 
TGCTTGGCCT ACCTGACTGC CCGTTTGCCC CAGGGATCGC TGGTCCCCTA CATGCCCAAC 
GCTGTGGAGC AGGCCAGTGA GGCTGTATAC AAACTCGCTG AATCAGGGTC TTCTTTGGGG 
AAAAAGGTGG CAGGTGCCCT ACAGACCATT GAGACCTCCC TGGCTCAGTA CAGCCTCACC 
CAGCTCTGTG TGGGCTTCAA CGGGGGCAAA GACTGCACTG CCCTCCTGCA CCTCTTCCAT 
GCAGCTGTGC AGAGGAAATT ACCTGATGTT CCAAACCCCC TCCAGATCCT GTATATCCGC 
AGCATCTCCC CTTTCCCTGA GCTGGAACAG TTTCTACAGG ACACTATCAA GAGGTATAAT 
CTGCAGATGT TGGAAGCTGA GGGCAGCATG AAGCAGGCCC TGGGTGAACT GCAGGCACGG 
CACCCCCAGC TGGAGGCTGT CCTTATGGGC ACCCGCCGGA CTGACCCCTA CTCCTGTAGC 
CTCTGCCCTT TCAGCCCCAC TGACCCAGGC TGGCCCGCAT TCATGCGCAT CAACCCACTG 
CTGGACTGGA CCTACAGAGA CATCTGGGAT TTTCTGCGTC AGCTGTTTGT CCCATACTGT 
ATCCTGTATG ACCGAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Riboflavin-Responsive and -Non-responsive Mutations in FAD Synthase Cause Multiple Acyl-CoA Dehydrogenase and Combined Respiratory-Chain Deficiency.
American journal of human genetics98(6)1130-1145(2016 Jun)
Olsen RKJ,Ko?a??kov? E,Giancaspero TA,Mosegaard S,Boczonadi V,Matakovi? L,Veauville-Merlli? A,Terrile C,Schwarzmayr T,Haack TB,Auranen M,Leone P,Galluccio M,Imbard A,Gutierrez-Rios P,Palmfeldt J,Graf E,Vianey-Saban C,Oppenheim M,Schiff M,Pichard S,Rigal O,Pyle A,Chinnery PF,Konstantopoulou V,M?slinger D,Feichtinger RG,Talim B,Topaloglu H,Coskun T,Gucer S,Botta A,Pegoraro E,Malena A,Vergani L,Mazz? D,Zollino M,Ghezzi D,Acquaviva C,Tyni T,Boneh A,Meitinger T,Strom TM,Gregersen N,Mayr JA,Horvath R,Barile M,Prokisch H

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Nature genetics45(4)385-91, 391e1-2(2013 Apr)
Eeles RA,Olama AA,Benlloch S,Saunders EJ,Leongamornlert DA,Tymrakiewicz M,Ghoussaini M,Luccarini C,Dennis J,Jugurnauth-Little S,Dadaev T,Neal DE,Hamdy FC,Donovan JL,Muir K,Giles GG,Severi G,Wiklund F,Gronberg H,Haiman CA,Schumacher F,Henderson BE,Le Marchand L,Lindstrom S,Kraft P,Hunter DJ,Gapstur S,Chanock SJ,Berndt SI,Albanes D,Andriole G,Schleutker J,Weischer M,Canzian F,Riboli E,Key TJ,Travis RC,Campa D,Ingles SA,John EM,Hayes RB,Pharoah PD,Pashayan N,Khaw KT,Stanford JL,Ostrander EA,Signorello LB,Thibodeau SN,Schaid D,Maier C,Vogel W,Kibel AS,Cybulski C,Lubinski J,Cannon-Albright L,Brenner H,Park JY,Kaneva R,Batra J,Spurdle AB,Clements JA,Teixeira MR,Dicks E,Lee A,Dunning AM,Baynes C,Conroy D,Maranian MJ,Ahmed S,Govindasami K,Guy M,Wilkinson RA,Sawyer EJ,Morgan A,Dearnaley DP,Horwich A,Huddart RA,Khoo VS,Parker CC,Van As NJ,Woodhouse CJ,Thompson A,Dudderidge T,Ogden C,Cooper CS,Lophatananon A,Cox A,Southey MC,Hopper JL,English DR,Aly M,Adolfsson J,Xu J,Zheng SL,Yeager M,Kaaks R,Diver WR,Gaudet MM,Stern MC,Corral R,Joshi AD,Shahabi A,Wahlfors T,Tammela TL,Auvinen A,Virtamo J,Klarskov P,Nordestgaard BG,R?der MA,Nielsen SF,Bojesen SE,Siddiq A,Fitzgerald LM,Kolb S,Kwon EM,Karyadi DM,Blot WJ,Zheng W,Cai Q,McDonnell SK,Rinckleb AE,Drake B,Colditz G,Wokolorczyk D,Stephenson RA,Teerlink C,Muller H,Rothenbacher D,Sellers TA,Lin HY,Slavov C,Mitev V,Lose F,Srinivasan S,Maia S,Paulo P,Lange E,Cooney KA,Antoniou AC,Vincent D,Bacot F,Tessier DC,,,,,,Kote-Jarai Z,Easton DF

Specific electron transport chain abnormalities in amyotrophic lateral sclerosis.
Journal of neurology256(5)774-82(2009 May)
Lin J,Diamanduros A,Chowdhury SA,Scelsa S,Latov N,Sadiq SA

Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase.
Biochemical and biophysical research communications344(3)1008-16(2006 Jun)
Brizio C,Galluccio M,Wait R,Torchetti EM,Bafunno V,Accardi R,Gianazza E,Indiveri C,Barile M

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FLAD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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