NAA50 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAA50
Entrez Gene ID	80218
Full Name	N(alpha)-acetyltransferase 50, NatE catalytic subunit
Synonyms	MAK3,NAT13,NAT13P,NAT5,NAT5P,SAN,hNaa50p
General protein information	Preferred Names N(alpha)-acetyltransferase 50, NatE catalytic subunit Names N-alpha-acetyltransferase 50 N-acetyltransferase 13 (GCN5-related) N-acetyltransferase 5 N-acetyltransferase san homolog N-epsilon-acetyltransferase 50 natE catalytic subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.31
Disorder MIM:	610834

mRNA and Protein(s)

mRNA	Protein	Name
NM_025146.4	NP_079422.1	N-alpha-acetyltransferase 50 isoform 1
NM_001308445.1	NP_001295374.1	N-alpha-acetyltransferase 50 isoform 2
NM_025146.3	NP_079422.1	N-alpha-acetyltransferase 50 isoform 1
NM_001308445.2	NP_001295374.1	N-alpha-acetyltransferase 50 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NAA50	XP_003640176.1
Gallus gallus (chicken)	NAA50	NP_001025949.1
Xenopus tropicalis (tropical clawed frog)	naa50	NP_001011131.1
Bos taurus (cattle)	NAA50	NP_001069218.1
Homo sapiens (human)	NAA50	NP_079422.1
Pan troglodytes (chimpanzee)	NAA50	XP_526267.3
	NAA50	XP_002802699.1
Rattus norvegicus (Norway rat)	Naa50	NP_001099351.1
Mus musculus (house mouse)	Naa50	NP_082384.1
Danio rerio (zebrafish)	naa50	NP_001003623.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Human Naa50 Protein Displays Broad Substrate Specificity for Amino-terminal Acetylation: DETAILED STRUCTURAL AND BIOCHEMICAL ANALYSIS USING TETRAPEPTIDE LIBRARY.
Reddi R, Saddanapu V, Chinthapalli DK, Sankoju P, Sripadi P, Addlagatta A
The Journal of biological chemistry291(39)20530-8(2016 09)

Opposing Functions of the N-terminal Acetyltransferases Naa50 and NatA in Sister-chromatid Cohesion.
Rong Z, Ouyang Z, Magin RS, Marmorstein R, Yu H
The Journal of biological chemistry291(36)19079-91(2016 09)

N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
Van Damme P, Hole K, Gevaert K, Arnesen T
Proteomics15(14)2436-46(2015 Jul)

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, St?ve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T
Cell reports10(8)1362-74(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Because Naa10 is reported to acetylate all amino termini that are devoid of methionine and Naa50 acetylates all other peptides that are followed by methionine
Title: Human Naa50 Protein Displays Broad Substrate Specificity for Amino-terminal Acetylation: DETAILED STRUCTURAL AND BIOCHEMICAL ANALYSIS USING TETRAPEPTIDE LIBRARY.

co-depletion of NatA, a heterodimeric NAT complex that physically interacts with Naa50, rescues the sister-chromatid cohesion defects and the resulting mitotic arrest caused by Naa50 depletion, indicating that NatA and Naa50 play antagonistic roles in cohesion.
Title: Opposing Functions of the N-terminal Acetyltransferases Naa50 and NatA in Sister-chromatid Cohesion.

The study quantitatively compared the Nt-acetylomes of wild-type yeast S. cerevisiae expressing the endogenous yeast Naa50 (yNaa50), the congenic strain lacking yNaa50, and an otherwise identical strain expressing human Naa50 (hNaa50).
Title: N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.

Development of the first N-terminal acetyltransferase (NAT) inhibitors, including a specific Naa50 inhibitor.
Title: Design, synthesis, and kinetic characterization of protein N-terminal acetyltransferase inhibitors.

Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study.
Title: Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study.

The following NAA50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAA50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15478	NM_025146.4 Latest version!	Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu38158	NM_001308445.2 Latest version!	Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu15478	NM_025146.3	Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu38158	NM_001308445.1	Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAA50 ( NM_025146.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079422.1 Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15478
Clone ID Related Accession (Same CDS sequence)	NM_025146.4 , NM_025146.3
Accession Version	NM_025146.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 50 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM831426.1, BC012731.2, DB198553.1, DB096143.1, DA408894.1, DA599265.1, HX784836.1, AW954963.1, H15889.1, H39948.1, BX102492.1 and AC108693.5. On Nov 22, 2018 this sequence version replaced NM_025146.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK023256.1, SRR1803612.19842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000240922.8/ ENSP00000240922.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA 
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG 
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC 
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG 
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT 
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA 
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG 
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG 
AATGCAGATG TGCAAAAGAC AGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079422.1
CDS	301..810
Translation	MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN

Target ORF information:

RefSeq Version	NM_025146.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025146.4

ORF Insert Sequence:

ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA 
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG 
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC 
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG 
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT 
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA 
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG 
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG 
AATGCAGATG TGCAAAAGAC AGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAA50 ( NM_025146.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079422.1 Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15478
Clone ID Related Accession (Same CDS sequence)	NM_025146.4 , NM_025146.3
Accession Version	NM_025146.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 50 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA854531.1, BM831426.1, BC012731.2, DB198553.1, DB096143.1, DA408894.1, DA599265.1, HX784836.1, AW954963.1, H15889.1, H39948.1 and BX102492.1. On May 5, 2015 this sequence version replaced NM_025146.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.19842.1, SRR1803615.249830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA 
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG 
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC 
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG 
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT 
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA 
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG 
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG 
AATGCAGATG TGCAAAAGAC AGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079422.1
CDS	351..860
Translation	MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN

Target ORF information:

RefSeq Version	NM_025146.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025146.3

ORF Insert Sequence:

ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA 
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG 
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC 
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG 
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT 
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA 
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG 
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG 
AATGCAGATG TGCAAAAGAC AGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAA50 ( NM_001308445.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295374.1 Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu38158
Clone ID Related Accession (Same CDS sequence)	NM_001308445.1 , NM_001308445.2
Accession Version	NM_001308445.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 50 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108693.5, AL601126.1 and BX102492.1. On May 5, 2015 this sequence version replaced XM_005247794.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.51106.1, SRR1660803.1249.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA 
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG 
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT 
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA 
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA 
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT 
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG 
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT 
GCAGATGTGC AAAAGACAGA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295374.1
CDS	351..857
Translation	MKGRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN

Target ORF information:

RefSeq Version	NM_001308445.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308445.1

ORF Insert Sequence:

ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA 
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG 
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT 
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA 
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA 
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT 
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG 
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT 
GCAGATGTGC AAAAGACAGA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAA50 ( NM_001308445.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295374.1 Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu38158
Clone ID Related Accession (Same CDS sequence)	NM_001308445.1 , NM_001308445.2
Accession Version	NM_001308445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 50 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108693.5, AL601126.1 and BX102492.1. On May 31, 2019 this sequence version replaced NM_001308445.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.20858.1, SRR1803614.121389.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA 
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG 
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT 
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA 
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA 
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT 
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG 
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT 
GCAGATGTGC AAAAGACAGA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295374.1
CDS	301..807
Translation	MKGRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN

Target ORF information:

RefSeq Version	NM_001308445.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308445.2

ORF Insert Sequence:

ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA 
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG 
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT 
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA 
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA 
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT 
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG 
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT 
GCAGATGTGC AAAAGACAGA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Human Naa50 Protein Displays Broad Substrate Specificity for Amino-terminal Acetylation: DETAILED STRUCTURAL AND BIOCHEMICAL ANALYSIS USING TETRAPEPTIDE LIBRARY.
The Journal of biological chemistry291(39)20530-8(2016 09)
Reddi R,Saddanapu V,Chinthapalli DK,Sankoju P,Sripadi P,Addlagatta A

Opposing Functions of the N-terminal Acetyltransferases Naa50 and NatA in Sister-chromatid Cohesion.
The Journal of biological chemistry291(36)19079-91(2016 09)
Rong Z,Ouyang Z,Magin RS,Marmorstein R,Yu H

N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
Proteomics15(14)2436-46(2015 Jul)
Van Damme P,Hole K,Gevaert K,Arnesen T

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Cell reports10(8)1362-74(2015 Mar)
Aksnes H,Van Damme P,Goris M,Starheim KK,Marie M,St?ve SI,Hoel C,Kalvik TV,Hole K,Glomnes N,Furnes C,Ljostveit S,Ziegler M,Niere M,Gevaert K,Arnesen T

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NAA50 cDNA ORF clone, Homo sapiens(human)

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