NUP214 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011519055.2	XP_011517357.1	nuclear pore complex protein Nup214 isoform X7
NM_005085.3	NP_005076.3	nuclear pore complex protein Nup214 isoform 2
XM_005272216.3	XP_005272273.1	nuclear pore complex protein Nup214 isoform X1
NM_001318325.2	NP_001305254.1	nuclear pore complex protein Nup214 isoform 4
NM_001318325.1	NP_001305254.1	nuclear pore complex protein Nup214 isoform 4
XM_006717292.3	XP_006717355.1	nuclear pore complex protein Nup214 isoform X2
XM_005272218.3	XP_005272275.1	nuclear pore complex protein Nup214 isoform X5
XM_005272219.3	XP_005272276.1	nuclear pore complex protein Nup214 isoform X6
XM_011519053.2	XP_011517355.1	nuclear pore complex protein Nup214 isoform X3
XM_017015167.1	XP_016870656.1	nuclear pore complex protein Nup214 isoform X4
NM_001318324.2	NP_001305253.1	nuclear pore complex protein Nup214 isoform 3
NM_001318324.1	NP_001305253.1	nuclear pore complex protein Nup214 isoform 3
NM_005085.4	NP_005076.3	nuclear pore complex protein Nup214 isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu100617	NM_001318325.2 Latest version!	Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu61586	XM_011519055.2 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu100669	NM_001318324.2 Latest version!	Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98936	XM_017015167.1 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu27642	NM_005085.4 Latest version!	Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu43524	XM_005272216.3 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43526	XM_006717292.3 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43527	XM_005272218.3 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43528	XM_005272219.3 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu61584	XM_011519053.2 Latest version!	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu100617	NM_001318325.1	Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100669	NM_001318324.1	Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu27642	NM_005085.3	Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

NUP214 ( NM_001318325.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305254.1 Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100617
Clone ID Related Accession (Same CDS sequence)	NM_001318325.2 , NM_001318325.1
Accession Version	NM_001318325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK316417.1, AL157938.22 and BC105998.1. On May 31, 2019 this sequence version replaced NM_001318325.1. Transcript Variant: This variant (4) lacks several exons and contains an alternate 5' terminal exon resulting in a distinct 5' UTR and 5' coding region, compared to variant 2. It encodes isoform 4, which is shorter and has a distinct N-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316417.1, AK302676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCT CTCTTTTGTT ACGAGGTGTT CACTTCTGTT TTCACTTGCC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305254.1
CDS	291..3041
Translation	MKSSLLLRGVHFCFHLPGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_001318325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318325.2

ORF Insert Sequence:

ATGAAATCCT CTCTTTTGTT ACGAGGTGTT CACTTCTGTT TTCACTTGCC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( NM_001318325.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305254.1 Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100617
Clone ID Related Accession (Same CDS sequence)	NM_001318325.2 , NM_001318325.1
Accession Version	NM_001318325.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157938.22, AK316417.1, BC105998.1 and AA516455.1. Transcript Variant: This variant (4) lacks several exons and contains an alternate 5' terminal exon resulting in a distinct 5' UTR and 5' coding region, compared to variant 2. It encodes isoform 4, which is shorter and has a distinct N-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316417.1, AK302676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCT CTCTTTTGTT ACGAGGTGTT CACTTCTGTT TTCACTTGCC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305254.1
CDS	315..3065
Translation	MKSSLLLRGVHFCFHLPGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_001318325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318325.1

ORF Insert Sequence:

ATGAAATCCT CTCTTTTGTT ACGAGGTGTT CACTTCTGTT TTCACTTGCC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_011519055.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517357.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X7, mRNA.

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CloneID	OHu61586
Clone ID Related Accession (Same CDS sequence)	XM_011519055.2
Accession Version	XM_011519055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGACAGCCAA GATAGAAACA 
GCTGTGACTT CAACCCCATC TGCTTCTGGG CAGTTCAGCA AGCCTTTCTC ATTTTCTCCA 
TCAGGGACTG GCTTTAATTT TGGGATAATC ACACCAACAC CGTCTTCTAA TTTCACTGCT 
GCACAAGGGG CAACACCCTC CACTAAAGAG TCAAGCCAGC CGGACGCATT CTCATCTGGT 
GGGGGAAGCA AACCTTCTTA TGAGGCCATT CCTGAAAGCT CACCTCCCTC AGGAATCACA 
TCCGCATCAA ACACCACCCC AGGAGAACCT GCCGCATCTA GCAGCAGACC TGTGGCACCT 
TCTGGAACTG CTCTTTCCAC CACCTCTAGT AAGCTGGAAA CCCCACCGTC CAAGCTGGGA 
GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA GAGACTCTGG GAAGTTTTTC AGGACTGCGG 
GTTGGCCAAG CAGATGATTC TACAAAACCA ACCAATAAGG CTTCATCCAC AAGCCTAACT 
AGTACCCAGC CAACCAAGAC GTCAGGCGTG CCCTCAGGGT TTAATTTTAC TGCCCCCCCG 
GTGTTAGGGA AGCACACGGA GCCCCCTGTG ACATCCTCTG CAACCACCAC CTCAGTAGCA 
CCACCAGCAG CCACCAGCAC TTCCTCAACT GCCGTTTTTG GCAGTCTGCC AGTCACCAGT 
GCAGGATCCT CTGGGGTCAT CAGTTTTGGT GGGACATCTC TAAGTGCTGG CAAGACTAGT 
TTTTCATTTG GAAGCCAACA GACCAATAGC ACAGTGCCCC CATCTGCCCC ACCACCAACT 
ACAGCTGCCA CTCCCCTTCC AACATCATTC CCCACATTGT CATTTGGTAG CCTCCTGAGT 
TCAGCAACTA CCCCCTCCCT GCCTATGTCC GCTGGCAGAA GCACAGAAGA GGCCACTTCA 
TCAGCTTTGC CTGAGAAGCC AGGTGACAGT GAGGTCTCAG CATCAGCAGC CTCACTTCTA 
GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG GCTCCTCCGC AAACTTCTGA CTCTGTTAAA 
AAAGAACCTG TTCTTGCCCA GCCTGCAGTC AGCAACTCTG GCACTGCAGC ATCTAGTACT 
AGTCTTGTAG CACTTTCTGC AGAGGCTACC CCAGCCACCA CGGGGGTCCC TGATGCCAGG 
ACGGAGGCAG TACCACCTGC TTCCTCCTTT TCTGTGCCTG GGCAGACTGC TGTCACAGCA 
GCTGCTATCT CAAGTGCAGG CCCTGTGGCC GTCGAAACAT CAAGTACCCC CATAGCCTCC 
AGCACCACGT CCATTGTTGC TCCCGGCCCA TCTGCAGAGG CAGCAGCATT TGGTACCGTC 
ACTTCTGGCT CATCCGTCTT TGCTCAGCCT CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC 
CAGCTCACCA ACAACACAGC CACTGCCCCC TCTGCCACGC CCGTGTTTGG GCAAGTGGCA 
GCCAGCACCG CACCAAGTCT GTTTGGGCAG CAGACTGGTA GCACAGCCAG CACAGCAGCT 
GCCACACCAC AGGTCAGCAG CTCAGGGTTT AGCAGCCCAG CTTTTGGTAC CACAGCCCCA 
GGGGTCTTTG GACAGACAAC CTTCGGGCAG GCCTCAGTCT TTGGGCAGTC GGCGAGCAGT 
GCTGCAAGTG TCTTTTCCTT CAGTCAGCCT GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG 
CCTGCTTCCT CCACTCCCAC ATCCACCAGT GGAAGTGTCT TTGGTGCCGC CTCAAGTACC 
AGTAGCTCCA GTTCCTTCTC ATTTGGACAG TCTTCTCCCA ACACAGGAGG GGGGCTGTTT 
GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG AGTCCTGGCT TTGGACAGGG AGGCTCTGTC 
TTTGGTGGTA CCTCAGCTGC CACCACAACA GCAGCAACCT CTGGGTTCAG CTTTTGCCAA 
GCTTCAGGTT TTGGGTCTAG TAATACTGGT TCTGTGTTTG GTCAAGCAGC CAGTACTGGT 
GGAATAGTCT TTGGCCAGTA TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517357.1
CDS	22..5748
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQYL

Target ORF information:

RefSeq Version	XM_011519055.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519055.2

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGACAGCCAA GATAGAAACA 
GCTGTGACTT CAACCCCATC TGCTTCTGGG CAGTTCAGCA AGCCTTTCTC ATTTTCTCCA 
TCAGGGACTG GCTTTAATTT TGGGATAATC ACACCAACAC CGTCTTCTAA TTTCACTGCT 
GCACAAGGGG CAACACCCTC CACTAAAGAG TCAAGCCAGC CGGACGCATT CTCATCTGGT 
GGGGGAAGCA AACCTTCTTA TGAGGCCATT CCTGAAAGCT CACCTCCCTC AGGAATCACA 
TCCGCATCAA ACACCACCCC AGGAGAACCT GCCGCATCTA GCAGCAGACC TGTGGCACCT 
TCTGGAACTG CTCTTTCCAC CACCTCTAGT AAGCTGGAAA CCCCACCGTC CAAGCTGGGA 
GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA GAGACTCTGG GAAGTTTTTC AGGACTGCGG 
GTTGGCCAAG CAGATGATTC TACAAAACCA ACCAATAAGG CTTCATCCAC AAGCCTAACT 
AGTACCCAGC CAACCAAGAC GTCAGGCGTG CCCTCAGGGT TTAATTTTAC TGCCCCCCCG 
GTGTTAGGGA AGCACACGGA GCCCCCTGTG ACATCCTCTG CAACCACCAC CTCAGTAGCA 
CCACCAGCAG CCACCAGCAC TTCCTCAACT GCCGTTTTTG GCAGTCTGCC AGTCACCAGT 
GCAGGATCCT CTGGGGTCAT CAGTTTTGGT GGGACATCTC TAAGTGCTGG CAAGACTAGT 
TTTTCATTTG GAAGCCAACA GACCAATAGC ACAGTGCCCC CATCTGCCCC ACCACCAACT 
ACAGCTGCCA CTCCCCTTCC AACATCATTC CCCACATTGT CATTTGGTAG CCTCCTGAGT 
TCAGCAACTA CCCCCTCCCT GCCTATGTCC GCTGGCAGAA GCACAGAAGA GGCCACTTCA 
TCAGCTTTGC CTGAGAAGCC AGGTGACAGT GAGGTCTCAG CATCAGCAGC CTCACTTCTA 
GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG GCTCCTCCGC AAACTTCTGA CTCTGTTAAA 
AAAGAACCTG TTCTTGCCCA GCCTGCAGTC AGCAACTCTG GCACTGCAGC ATCTAGTACT 
AGTCTTGTAG CACTTTCTGC AGAGGCTACC CCAGCCACCA CGGGGGTCCC TGATGCCAGG 
ACGGAGGCAG TACCACCTGC TTCCTCCTTT TCTGTGCCTG GGCAGACTGC TGTCACAGCA 
GCTGCTATCT CAAGTGCAGG CCCTGTGGCC GTCGAAACAT CAAGTACCCC CATAGCCTCC 
AGCACCACGT CCATTGTTGC TCCCGGCCCA TCTGCAGAGG CAGCAGCATT TGGTACCGTC 
ACTTCTGGCT CATCCGTCTT TGCTCAGCCT CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC 
CAGCTCACCA ACAACACAGC CACTGCCCCC TCTGCCACGC CCGTGTTTGG GCAAGTGGCA 
GCCAGCACCG CACCAAGTCT GTTTGGGCAG CAGACTGGTA GCACAGCCAG CACAGCAGCT 
GCCACACCAC AGGTCAGCAG CTCAGGGTTT AGCAGCCCAG CTTTTGGTAC CACAGCCCCA 
GGGGTCTTTG GACAGACAAC CTTCGGGCAG GCCTCAGTCT TTGGGCAGTC GGCGAGCAGT 
GCTGCAAGTG TCTTTTCCTT CAGTCAGCCT GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG 
CCTGCTTCCT CCACTCCCAC ATCCACCAGT GGAAGTGTCT TTGGTGCCGC CTCAAGTACC 
AGTAGCTCCA GTTCCTTCTC ATTTGGACAG TCTTCTCCCA ACACAGGAGG GGGGCTGTTT 
GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG AGTCCTGGCT TTGGACAGGG AGGCTCTGTC 
TTTGGTGGTA CCTCAGCTGC CACCACAACA GCAGCAACCT CTGGGTTCAG CTTTTGCCAA 
GCTTCAGGTT TTGGGTCTAG TAATACTGGT TCTGTGTTTG GTCAAGCAGC CAGTACTGGT 
GGAATAGTCT TTGGCCAGTA TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( NM_001318324.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305253.1 Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.

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CloneID	OHu100669
Clone ID Related Accession (Same CDS sequence)	NM_001318324.2 , NM_001318324.1
Accession Version	NM_001318324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC105998.1 and AL157938.22. On Jun 3, 2019 this sequence version replaced NM_001318324.1. Transcript Variant: This variant (3) uses two alternate splice sites at internal exons, compared to variant 2. It encodes isoform 3, which is shorter than isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305253.1
CDS	120..6362
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSENSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_001318324.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318324.2

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( NM_001318324.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305253.1 Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.

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CloneID	OHu100669
Clone ID Related Accession (Same CDS sequence)	NM_001318324.2 , NM_001318324.1
Accession Version	NM_001318324.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062584.1, BC105998.1, AL157938.22 and AA516455.1. On Jan 1, 2016 this sequence version replaced XM_005272217.2. Transcript Variant: This variant (3) uses two alternate splice sites at internal exons, compared to variant 2. It encodes isoform 3, which is shorter than isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:17264208) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305253.1
CDS	145..6387
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSENSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_001318324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318324.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_017015167.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870656.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X4, mRNA.

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CloneID	OHu98936
Clone ID Related Accession (Same CDS sequence)	XM_017015167.1
Accession Version	XM_017015167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AACTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGGACGATCT 
GCTCAGGGCA GTTCAAGCCC AGTGCCCTCA ATGGTACAGA AATCACCCAG GATAACCCCT 
CCAGCGGCAA AGCCAGGCTC TCCCCAGGCA AAGTCACTTC AGCCTGCTGT TGCAGAAAAG 
CAGGGACATC AGTGGAAAGA TTCAGATCCT GTAATGGCTG GAATTGGGGA GGAGATTGCA 
CACTTTCAGA AGGAGTTGGA AGAGTTAAAA GCCCGAACTT CCAAAGCCTG TTTCCAAGTG 
GGCACTTCTG AGGAGATGAA GATGCTGCGA ACAGAATCAG ATGACTTGCA TACCTTTCTT 
TTGGAGATTA AAGAGACCAC AGAGTCGCTT CATGGAGATA TAAGTAGCCT GAAAACAACT 
TTACTTGAGG GCTTTGCTGG TGTTGAGGAA GCCAGAGAAC AAAATGAAAG AAATCGTGAC 
TCTGGTTATC TGCATTTGCT TTATAAAAGA CCACTGGATC CCAAGAGTGA AGCTCAGCTT 
CAGGAAATTC GGCGCCTTCA TCAGTATGTG AAATTTGCTG TCCAAGATGT GAATGATGTT 
CTAGACTTGG AGTGGGATCA GCATCTGGAA CAAAAGAAAA AACAAAGGCA CCTGCTTGTG 
CCAGAGCGAG AGACACTGTT TAACACCCTA GCCAACAATC GGGAAATCAT CAACCAACAG 
AGGAAGAGGC TGAATCACCT GGTGGATAGT CTTCAGCAGC TCCGCCTTTA CAAACAGACT 
TCCCTGTGGA GCCTGTCCTC GGCTGTTCCT TCCCAGAGCA GCATTCACAG TTTTGACAGT 
GACCTGGAAA GCCTGTGCAA TGCTTTGTTG AAAACCACCA TAGAATCTCA CACCAAATCC 
TTGCCCAAAG TACCAGCCAA ACTGTCCCCC ATGAAACAGG CACAACTGAG AAACTTCTTG 
GCCAAGAGGA AGACCCCACC AGTGAGATCC ACTGCTCCAG CCAGCCTGTC TCGATCAGCC 
TTTCTGTCTC AGAGATATTA TGAAGACTTG GATGAAGTCA GCTCAACGTC ATCTGTCTCC 
CAGTCTCTGG AGAGTGAAGA TGCACGGACG TCCTGTAAAG ATGACGAGGC AGTGGTTCAG 
GCCCCTCGGC ACGCCCCCGT GGTTCGCACT CCTTCCATCC AGCCCAGTCT CTTGCCCCAT 
GCAGCACCTT TTGCTAAATC TCACCTGGTT CATGGTTCTT CACCTGGTGT GATGGGAACT 
TCAGTGGCTA CATCTGCTAG CAAAATTATT CCTCAAGGGG CCGATAGCAC AATGCTTGCC 
ACGAAAACCG TGAAACATGG TGCACCTAGT CCTTCCCACC CCATCTCAGC CCCGCAGGCA 
GCTGCCGCAG CAGCACTCAG GCGGCAGATG GCCAGTCAGG CACCAGCTGT AAACACTTTG 
ACTGAATCAA CGTTGAAGAA TGTCCCTCAA GTGGTAAATG TGCAGGAATT GAAGAATAAC 
CCTGCAACCC CTTCTACAGC CATGGGTTCT TCAGTGCCCT ACTCCACAGC CAAAACACCT 
CACCCAGTGT TGACCCCAGT GGCTGCTAAC CAAGCCAAGC AGGGGTCTCT AATAAATTCC 
CTTAAGCCAT CTGGGCCTAC ACCAGCATCC GGTCAGTTAT CATCTGGTGA CAAAGCTTCA 
GGGACAGCCA AGATAGAAAC AGCTGTGACT TCAACCCCAT CTGCTTCTGG GCAGTTCAGC 
AAGCCTTTCT CATTTTCTCC ATCAGGGACT GGCTTTAATT TTGGGATAAT CACACCAACA 
CCGTCTTCTA ATTTCACTGC TGCACAAGGG GCAACACCCT CCACTAAAGA GTCAAGCCAG 
CCGGACGCAT TCTCATCTGG TGGGGGAAGC AAACCTTCTT ATGAGGCCAT TCCTGAAAGC 
TCACCTCCCT CAGGAATCAC ATCCGCATCA AACACCACCC CAGGAGAACC TGCCGCATCT 
AGCAGCAGAC CTGTGGCACC TTCTGGAACT GCTCTTTCCA CCACCTCTAG TAAGCTGGAA 
ACCCCACCGT CCAAGCTGGG AGAGCTTCTG TTTCCAAGTT CTTTGGCTGG AGAGACTCTG 
GGAAGTTTTT CAGGACTGCG GGTTGGCCAA GCAGATGATT CTACAAAACC AACCAATAAG 
GCTTCATCCA CAAGCCTAAC TAGTACCCAG CCAACCAAGA CGTCAGGCGT GCCCTCAGGG 
TTTAATTTTA CTGCCCCCCC GGTGTTAGGG AAGCACACGG AGCCCCCTGT GACATCCTCT 
GCAACCACCA CCTCAGTAGC ACCACCAGCA GCCACCAGCA CTTCCTCAAC TGCCGTTTTT 
GGCAGTCTGC CAGTCACCAG TGCAGGATCC TCTGGGGTCA TCAGTTTTGG TGGGACATCT 
CTAAGTGCTG GCAAGACTAG TTTTTCATTT GGAAGCCAAC AGACCAATAG CACAGTGCCC 
CCATCTGCCC CACCACCAAC TACAGCTGCC ACTCCCCTTC CAACATCATT CCCCACATTG 
TCATTTGGTA GCCTCCTGAG TTCAGCAACT ACCCCCTCCC TGCCTATGTC CGCTGGCAGA 
AGCACAGAAG AGGCCACTTC ATCAGCTTTG CCTGAGAAGC CAGGTGACAG TGAGGTCTCA 
GCATCAGCAG CCTCACTTCT AGAGGAGCAA CAGTCAGCCC AGCTTCCCCA GGCTCCTCCG 
CAAACTTCTG ACTCTGTTAA AAAAGAACCT GTTCTTGCCC AGCCTGCAGT CAGCAACTCT 
GGCACTGCAG CATCTAGTAC TAGTCTTGTA GCACTTTCTG CAGAGGCTAC CCCAGCCACC 
ACGGGGGTCC CTGATGCCAG GACGGAGGCA GTACCACCTG CTTCCTCCTT TTCTGTGCCT 
GGGCAGACTG CTGTCACAGC AGCTGCTATC TCAAGTGCAG GCCCTGTGGC CGTCGAAACA 
TCAAGTACCC CCATAGCCTC CAGCACCACG TCCATTGTTG CTCCCGGCCC ATCTGCAGAG 
GCAGCAGCAT TTGGTACCGT CACTTCTGGC TCATCCGTCT TTGCTCAGCC TCCTGCTGCC 
AGTTCTAGCT CAGCTTTCAA CCAGCTCACC AACAACACAG CCACTGCCCC CTCTGCCACG 
CCCGTGTTTG GGCAAGTGGC AGCCAGCACC GCACCAAGTC TGTTTGGGCA GCAGACTGGT 
AGCACAGCCA GCACAGCAGC TGCCACACCA CAGGTCAGCA GCTCAGGGTT TAGCAGCCCA 
GCTTTTGGTA CCACAGCCCC AGGGGTCTTT GGACAGACAA CCTTCGGGCA GGCCTCAGTC 
TTTGGGCAGT CGGCGAGCAG TGCTGCAAGT GTCTTTTCCT TCAGTCAGCC TGGGTTCAGT 
TCCGTGCCTG CCTTCGGTCA GCCTGCTTCC TCCACTCCCA CATCCACCAG TGGAAGTGTC 
TTTGGTGCCG CCTCAAGTAC CAGTAGCTCC AGTTCCTTCT CATTTGGACA GTCTTCTCCC 
AACACAGGAG GGGGGCTGTT TGGCCAAAGC AACGCTCCTG CTTTTGGGCA GAGTCCTGGC 
TTTGGACAGG GAGGCTCTGT CTTTGGTGGT ACCTCAGCTG CCACCACAAC AGCAGCAACC 
TCTGGGTTCA GCTTTTGCCA AGCTTCAGGT TTTGGGTCTA GTAATACTGG TTCTGTGTTT 
GGTCAAGCAG CCAGTACTGG TGGAATAGTC TTTGGCCAGC AATCATCCTC TTCCAGTGGT 
AGCGTGTTTG GGTCTGGAAA CACTGGAAGA GGGGGAGGTT TCTTCAGTGG CCTTGGAGGA 
AAACCCAGTC AGGATGCAGC CAACAAAAAC CCATTCAGCT CGGCCAGTGG GGGCTTTGGA 
TCCACAGCTA CCTCAAATAC CTCTAACCTA TTTGGAAACA GTGGGGCCAA GACATTTGGT 
GGATTTGCCA GCTCGTCGTT TGGAGAGCAG AAACCCACTG GCACTTTCAG CTCTGGAGGA 
GGAAGTGTGG CATCCCAAGG CTTTGGGTTT TCCTCTCCAA ACAAAACAGG TGGCTTCGGT 
GCTGCTCCAG TGTTTGGCAG CCCTCCTACT TTTGGGGGAT CCCCTGGGTT TGGAGGGGTG 
CCAGCATTCG GTTCAGCCCC AGCCTTTACA AGCCCTCTGG GCTCGACGGG AGGCAAAGTG 
TTCGGAGAGG GCACTGCAGC TGCCAGCGCA GGAGGATTCG GGTTTGGGAG CAGCAGCAAC 
ACCACATCCT TCGGCACGCT CGCGAGTCAG AATGCCCCCA CTTTCGGATC ACTGTCCCAA 
CAGACTTCTG GTTTTGGGAC CCAGAGTAGC GGATTCTCTG GTTTTGGATC AGGCACAGGA 
GGGTTCAGCT TTGGGTCAAA TAACTCGTCT GTCCAGGGTT TTGGTGGCTG GCGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870656.1
CDS	22..6381
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSENSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_017015167.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015167.1

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AACTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGGACGATCT 
GCTCAGGGCA GTTCAAGCCC AGTGCCCTCA ATGGTACAGA AATCACCCAG GATAACCCCT 
CCAGCGGCAA AGCCAGGCTC TCCCCAGGCA AAGTCACTTC AGCCTGCTGT TGCAGAAAAG 
CAGGGACATC AGTGGAAAGA TTCAGATCCT GTAATGGCTG GAATTGGGGA GGAGATTGCA 
CACTTTCAGA AGGAGTTGGA AGAGTTAAAA GCCCGAACTT CCAAAGCCTG TTTCCAAGTG 
GGCACTTCTG AGGAGATGAA GATGCTGCGA ACAGAATCAG ATGACTTGCA TACCTTTCTT 
TTGGAGATTA AAGAGACCAC AGAGTCGCTT CATGGAGATA TAAGTAGCCT GAAAACAACT 
TTACTTGAGG GCTTTGCTGG TGTTGAGGAA GCCAGAGAAC AAAATGAAAG AAATCGTGAC 
TCTGGTTATC TGCATTTGCT TTATAAAAGA CCACTGGATC CCAAGAGTGA AGCTCAGCTT 
CAGGAAATTC GGCGCCTTCA TCAGTATGTG AAATTTGCTG TCCAAGATGT GAATGATGTT 
CTAGACTTGG AGTGGGATCA GCATCTGGAA CAAAAGAAAA AACAAAGGCA CCTGCTTGTG 
CCAGAGCGAG AGACACTGTT TAACACCCTA GCCAACAATC GGGAAATCAT CAACCAACAG 
AGGAAGAGGC TGAATCACCT GGTGGATAGT CTTCAGCAGC TCCGCCTTTA CAAACAGACT 
TCCCTGTGGA GCCTGTCCTC GGCTGTTCCT TCCCAGAGCA GCATTCACAG TTTTGACAGT 
GACCTGGAAA GCCTGTGCAA TGCTTTGTTG AAAACCACCA TAGAATCTCA CACCAAATCC 
TTGCCCAAAG TACCAGCCAA ACTGTCCCCC ATGAAACAGG CACAACTGAG AAACTTCTTG 
GCCAAGAGGA AGACCCCACC AGTGAGATCC ACTGCTCCAG CCAGCCTGTC TCGATCAGCC 
TTTCTGTCTC AGAGATATTA TGAAGACTTG GATGAAGTCA GCTCAACGTC ATCTGTCTCC 
CAGTCTCTGG AGAGTGAAGA TGCACGGACG TCCTGTAAAG ATGACGAGGC AGTGGTTCAG 
GCCCCTCGGC ACGCCCCCGT GGTTCGCACT CCTTCCATCC AGCCCAGTCT CTTGCCCCAT 
GCAGCACCTT TTGCTAAATC TCACCTGGTT CATGGTTCTT CACCTGGTGT GATGGGAACT 
TCAGTGGCTA CATCTGCTAG CAAAATTATT CCTCAAGGGG CCGATAGCAC AATGCTTGCC 
ACGAAAACCG TGAAACATGG TGCACCTAGT CCTTCCCACC CCATCTCAGC CCCGCAGGCA 
GCTGCCGCAG CAGCACTCAG GCGGCAGATG GCCAGTCAGG CACCAGCTGT AAACACTTTG 
ACTGAATCAA CGTTGAAGAA TGTCCCTCAA GTGGTAAATG TGCAGGAATT GAAGAATAAC 
CCTGCAACCC CTTCTACAGC CATGGGTTCT TCAGTGCCCT ACTCCACAGC CAAAACACCT 
CACCCAGTGT TGACCCCAGT GGCTGCTAAC CAAGCCAAGC AGGGGTCTCT AATAAATTCC 
CTTAAGCCAT CTGGGCCTAC ACCAGCATCC GGTCAGTTAT CATCTGGTGA CAAAGCTTCA 
GGGACAGCCA AGATAGAAAC AGCTGTGACT TCAACCCCAT CTGCTTCTGG GCAGTTCAGC 
AAGCCTTTCT CATTTTCTCC ATCAGGGACT GGCTTTAATT TTGGGATAAT CACACCAACA 
CCGTCTTCTA ATTTCACTGC TGCACAAGGG GCAACACCCT CCACTAAAGA GTCAAGCCAG 
CCGGACGCAT TCTCATCTGG TGGGGGAAGC AAACCTTCTT ATGAGGCCAT TCCTGAAAGC 
TCACCTCCCT CAGGAATCAC ATCCGCATCA AACACCACCC CAGGAGAACC TGCCGCATCT 
AGCAGCAGAC CTGTGGCACC TTCTGGAACT GCTCTTTCCA CCACCTCTAG TAAGCTGGAA 
ACCCCACCGT CCAAGCTGGG AGAGCTTCTG TTTCCAAGTT CTTTGGCTGG AGAGACTCTG 
GGAAGTTTTT CAGGACTGCG GGTTGGCCAA GCAGATGATT CTACAAAACC AACCAATAAG 
GCTTCATCCA CAAGCCTAAC TAGTACCCAG CCAACCAAGA CGTCAGGCGT GCCCTCAGGG 
TTTAATTTTA CTGCCCCCCC GGTGTTAGGG AAGCACACGG AGCCCCCTGT GACATCCTCT 
GCAACCACCA CCTCAGTAGC ACCACCAGCA GCCACCAGCA CTTCCTCAAC TGCCGTTTTT 
GGCAGTCTGC CAGTCACCAG TGCAGGATCC TCTGGGGTCA TCAGTTTTGG TGGGACATCT 
CTAAGTGCTG GCAAGACTAG TTTTTCATTT GGAAGCCAAC AGACCAATAG CACAGTGCCC 
CCATCTGCCC CACCACCAAC TACAGCTGCC ACTCCCCTTC CAACATCATT CCCCACATTG 
TCATTTGGTA GCCTCCTGAG TTCAGCAACT ACCCCCTCCC TGCCTATGTC CGCTGGCAGA 
AGCACAGAAG AGGCCACTTC ATCAGCTTTG CCTGAGAAGC CAGGTGACAG TGAGGTCTCA 
GCATCAGCAG CCTCACTTCT AGAGGAGCAA CAGTCAGCCC AGCTTCCCCA GGCTCCTCCG 
CAAACTTCTG ACTCTGTTAA AAAAGAACCT GTTCTTGCCC AGCCTGCAGT CAGCAACTCT 
GGCACTGCAG CATCTAGTAC TAGTCTTGTA GCACTTTCTG CAGAGGCTAC CCCAGCCACC 
ACGGGGGTCC CTGATGCCAG GACGGAGGCA GTACCACCTG CTTCCTCCTT TTCTGTGCCT 
GGGCAGACTG CTGTCACAGC AGCTGCTATC TCAAGTGCAG GCCCTGTGGC CGTCGAAACA 
TCAAGTACCC CCATAGCCTC CAGCACCACG TCCATTGTTG CTCCCGGCCC ATCTGCAGAG 
GCAGCAGCAT TTGGTACCGT CACTTCTGGC TCATCCGTCT TTGCTCAGCC TCCTGCTGCC 
AGTTCTAGCT CAGCTTTCAA CCAGCTCACC AACAACACAG CCACTGCCCC CTCTGCCACG 
CCCGTGTTTG GGCAAGTGGC AGCCAGCACC GCACCAAGTC TGTTTGGGCA GCAGACTGGT 
AGCACAGCCA GCACAGCAGC TGCCACACCA CAGGTCAGCA GCTCAGGGTT TAGCAGCCCA 
GCTTTTGGTA CCACAGCCCC AGGGGTCTTT GGACAGACAA CCTTCGGGCA GGCCTCAGTC 
TTTGGGCAGT CGGCGAGCAG TGCTGCAAGT GTCTTTTCCT TCAGTCAGCC TGGGTTCAGT 
TCCGTGCCTG CCTTCGGTCA GCCTGCTTCC TCCACTCCCA CATCCACCAG TGGAAGTGTC 
TTTGGTGCCG CCTCAAGTAC CAGTAGCTCC AGTTCCTTCT CATTTGGACA GTCTTCTCCC 
AACACAGGAG GGGGGCTGTT TGGCCAAAGC AACGCTCCTG CTTTTGGGCA GAGTCCTGGC 
TTTGGACAGG GAGGCTCTGT CTTTGGTGGT ACCTCAGCTG CCACCACAAC AGCAGCAACC 
TCTGGGTTCA GCTTTTGCCA AGCTTCAGGT TTTGGGTCTA GTAATACTGG TTCTGTGTTT 
GGTCAAGCAG CCAGTACTGG TGGAATAGTC TTTGGCCAGC AATCATCCTC TTCCAGTGGT 
AGCGTGTTTG GGTCTGGAAA CACTGGAAGA GGGGGAGGTT TCTTCAGTGG CCTTGGAGGA 
AAACCCAGTC AGGATGCAGC CAACAAAAAC CCATTCAGCT CGGCCAGTGG GGGCTTTGGA 
TCCACAGCTA CCTCAAATAC CTCTAACCTA TTTGGAAACA GTGGGGCCAA GACATTTGGT 
GGATTTGCCA GCTCGTCGTT TGGAGAGCAG AAACCCACTG GCACTTTCAG CTCTGGAGGA 
GGAAGTGTGG CATCCCAAGG CTTTGGGTTT TCCTCTCCAA ACAAAACAGG TGGCTTCGGT 
GCTGCTCCAG TGTTTGGCAG CCCTCCTACT TTTGGGGGAT CCCCTGGGTT TGGAGGGGTG 
CCAGCATTCG GTTCAGCCCC AGCCTTTACA AGCCCTCTGG GCTCGACGGG AGGCAAAGTG 
TTCGGAGAGG GCACTGCAGC TGCCAGCGCA GGAGGATTCG GGTTTGGGAG CAGCAGCAAC 
ACCACATCCT TCGGCACGCT CGCGAGTCAG AATGCCCCCA CTTTCGGATC ACTGTCCCAA 
CAGACTTCTG GTTTTGGGAC CCAGAGTAGC GGATTCTCTG GTTTTGGATC AGGCACAGGA 
GGGTTCAGCT TTGGGTCAAA TAACTCGTCT GTCCAGGGTT TTGGTGGCTG GCGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( NM_005085.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005076.3 Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.

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CloneID	OHu27642
Clone ID Related Accession (Same CDS sequence)	NM_005085.3 , NM_005085.4
Accession Version	NM_005085.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062584.1, AB159230.1, BC045620.1, BC105998.1 and AA516455.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2013 this sequence version replaced NM_005085.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X64228.1, SRR1803612.247797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:17264208) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCTG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CCCGATGTCG CCATTCTCTT CTGCCTCCAA GCCAGCTGCT 
TCTGGACCAC TCAGCCACCC CACACCTCTC TCAGCACCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ATATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGACT TGGAGTGGGA TCAGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ATCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAT 
AGTCTTCAGC AGCTCCGCCT TTACAAACAG ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTGG AAAGCCTGTG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTGTA AAGATGACGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGC CCTACTCCAC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCTGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT TCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCAACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCCGGACG CATTCTCATC TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCGCA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACCACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGGGTTGGC 
CAAGCAGATG ATTCTACAAA ACCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCGGTGTTA 
GGGAAGCACA CGGAGCCCCC TGTGACATCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGCCGTT TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCACC AACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCATTTG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCGCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAGCC ACCACGGGGG TCCCTGATGC CAGGACGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTA CCCCCATAGC CTCCAGCACC 
ACGTCCATTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCCG TCTTTGCTCA GCCTCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CCCCTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACCGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GTACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA 
AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACTC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT CCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGCA ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAATCATC CTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC AGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGGTC AAATAACTCG 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005076.3
CDS	145..6417
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_005085.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005085.3

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCTG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CCCGATGTCG CCATTCTCTT CTGCCTCCAA GCCAGCTGCT 
TCTGGACCAC TCAGCCACCC CACACCTCTC TCAGCACCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ATATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGACT TGGAGTGGGA TCAGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ATCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAT 
AGTCTTCAGC AGCTCCGCCT TTACAAACAG ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTGG AAAGCCTGTG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTGTA AAGATGACGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGC CCTACTCCAC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCTGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT TCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCAACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCCGGACG CATTCTCATC TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCGCA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACCACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGGGTTGGC 
CAAGCAGATG ATTCTACAAA ACCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCGGTGTTA 
GGGAAGCACA CGGAGCCCCC TGTGACATCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGCCGTT TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCACC AACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCATTTG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCGCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAGCC ACCACGGGGG TCCCTGATGC CAGGACGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTA CCCCCATAGC CTCCAGCACC 
ACGTCCATTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCCG TCTTTGCTCA GCCTCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CCCCTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACCGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GTACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA 
AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACTC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT CCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGCA ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAATCATC CTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC AGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGGTC AAATAACTCG 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( NM_005085.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005076.3 Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.

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CloneID	OHu27642
Clone ID Related Accession (Same CDS sequence)	NM_005085.3 , NM_005085.4
Accession Version	NM_005085.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062584.1, AB159230.1, BC045620.1 and BC105998.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_005085.3. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X64228.1, SRR1803612.247797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359428.10/ ENSP00000352400.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCTG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CCCGATGTCG CCATTCTCTT CTGCCTCCAA GCCAGCTGCT 
TCTGGACCAC TCAGCCACCC CACACCTCTC TCAGCACCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ATATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGACT TGGAGTGGGA TCAGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ATCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAT 
AGTCTTCAGC AGCTCCGCCT TTACAAACAG ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTGG AAAGCCTGTG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTGTA AAGATGACGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGC CCTACTCCAC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCTGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT TCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCAACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCCGGACG CATTCTCATC TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCGCA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACCACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGGGTTGGC 
CAAGCAGATG ATTCTACAAA ACCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCGGTGTTA 
GGGAAGCACA CGGAGCCCCC TGTGACATCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGCCGTT TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCACC AACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCATTTG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCGCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAGCC ACCACGGGGG TCCCTGATGC CAGGACGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTA CCCCCATAGC CTCCAGCACC 
ACGTCCATTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCCG TCTTTGCTCA GCCTCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CCCCTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACCGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GTACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA 
AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACTC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT CCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGCA ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAATCATC CTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC AGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGGTC AAATAACTCG 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005076.3
CDS	120..6392
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	NM_005085.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214 (NUP214), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005085.4

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTTGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTCGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAACAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGCGTGC ATGATGTCCA GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGATTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCCATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTT ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGTCGT AGACTATACA AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCAACCTCC 
TCTCAAGCCC CACAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTGCCACCT 
TCATCACCTG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTA CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCAG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCTGTGGCT 
CCATCAGCTG CTTCATTCTC CTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAATGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCTG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CCCGATGTCG CCATTCTCTT CTGCCTCCAA GCCAGCTGCT 
TCTGGACCAC TCAGCCACCC CACACCTCTC TCAGCACCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ATATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGACT TGGAGTGGGA TCAGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ATCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAT 
AGTCTTCAGC AGCTCCGCCT TTACAAACAG ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTGG AAAGCCTGTG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTGTA AAGATGACGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGC CCTACTCCAC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCTGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT TCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCAACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCCGGACG CATTCTCATC TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCGCA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACCACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGGGTTGGC 
CAAGCAGATG ATTCTACAAA ACCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCGGTGTTA 
GGGAAGCACA CGGAGCCCCC TGTGACATCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGCCGTT TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCACC AACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCATTTG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCGCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAGCC ACCACGGGGG TCCCTGATGC CAGGACGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTA CCCCCATAGC CTCCAGCACC 
ACGTCCATTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCCG TCTTTGCTCA GCCTCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CCCCTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACCGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GTACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA 
AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACTC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT CCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGCA ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAATCATC CTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC AGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGGTC AAATAACTCG 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_005272216.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272273.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

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CloneID	OHu43524
Clone ID Related Accession (Same CDS sequence)	XM_005272216.3
Accession Version	XM_005272216.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGACAGCCAA GATAGAAACA 
GCTGTGACTT CAACCCCATC TGCTTCTGGG CAGTTCAGCA AGCCTTTCTC ATTTTCTCCA 
TCAGGGACTG GCTTTAATTT TGGGATAATC ACACCAACAC CGTCTTCTAA TTTCACTGCT 
GCACAAGGGG CAACACCCTC CACTAAAGAG TCAAGCCAGC CGGACGCATT CTCATCTGGT 
GGGGGAAGCA AACCTTCTTA TGAGGCCATT CCTGAAAGCT CACCTCCCTC AGGAATCACA 
TCCGCATCAA ACACCACCCC AGGAGAACCT GCCGCATCTA GCAGCAGACC TGTGGCACCT 
TCTGGAACTG CTCTTTCCAC CACCTCTAGT AAGCTGGAAA CCCCACCGTC CAAGCTGGGA 
GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA GAGACTCTGG GAAGTTTTTC AGGACTGCGG 
GTTGGCCAAG CAGATGATTC TACAAAACCA ACCAATAAGG CTTCATCCAC AAGCCTAACT 
AGTACCCAGC CAACCAAGAC GTCAGGCGTG CCCTCAGGGT TTAATTTTAC TGCCCCCCCG 
GTGTTAGGGA AGCACACGGA GCCCCCTGTG ACATCCTCTG CAACCACCAC CTCAGTAGCA 
CCACCAGCAG CCACCAGCAC TTCCTCAACT GCCGTTTTTG GCAGTCTGCC AGTCACCAGT 
GCAGGATCCT CTGGGGTCAT CAGTTTTGGT GGGACATCTC TAAGTGCTGG CAAGACTAGT 
TTTTCATTTG GAAGCCAACA GACCAATAGC ACAGTGCCCC CATCTGCCCC ACCACCAACT 
ACAGCTGCCA CTCCCCTTCC AACATCATTC CCCACATTGT CATTTGGTAG CCTCCTGAGT 
TCAGCAACTA CCCCCTCCCT GCCTATGTCC GCTGGCAGAA GCACAGAAGA GGCCACTTCA 
TCAGCTTTGC CTGAGAAGCC AGGTGACAGT GAGGTCTCAG CATCAGCAGC CTCACTTCTA 
GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG GCTCCTCCGC AAACTTCTGA CTCTGTTAAA 
AAAGAACCTG TTCTTGCCCA GCCTGCAGTC AGCAACTCTG GCACTGCAGC ATCTAGTACT 
AGTCTTGTAG CACTTTCTGC AGAGGCTACC CCAGCCACCA CGGGGGTCCC TGATGCCAGG 
ACGGAGGCAG TACCACCTGC TTCCTCCTTT TCTGTGCCTG GGCAGACTGC TGTCACAGCA 
GCTGCTATCT CAAGTGCAGG CCCTGTGGCC GTCGAAACAT CAAGTACCCC CATAGCCTCC 
AGCACCACGT CCATTGTTGC TCCCGGCCCA TCTGCAGAGG CAGCAGCATT TGGTACCGTC 
ACTTCTGGCT CATCCGTCTT TGCTCAGCCT CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC 
CAGCTCACCA ACAACACAGC CACTGCCCCC TCTGCCACGC CCGTGTTTGG GCAAGTGGCA 
GCCAGCACCG CACCAAGTCT GTTTGGGCAG CAGACTGGTA GCACAGCCAG CACAGCAGCT 
GCCACACCAC AGGTCAGCAG CTCAGGGTTT AGCAGCCCAG CTTTTGGTAC CACAGCCCCA 
GGGGTCTTTG GACAGACAAC CTTCGGGCAG GCCTCAGTCT TTGGGCAGTC GGCGAGCAGT 
GCTGCAAGTG TCTTTTCCTT CAGTCAGCCT GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG 
CCTGCTTCCT CCACTCCCAC ATCCACCAGT GGAAGTGTCT TTGGTGCCGC CTCAAGTACC 
AGTAGCTCCA GTTCCTTCTC ATTTGGACAG TCTTCTCCCA ACACAGGAGG GGGGCTGTTT 
GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG AGTCCTGGCT TTGGACAGGG AGGCTCTGTC 
TTTGGTGGTA CCTCAGCTGC CACCACAACA GCAGCAACCT CTGGGTTCAG CTTTTGCCAA 
GCTTCAGGTT TTGGGTCTAG TAATACTGGT TCTGTGTTTG GTCAAGCAGC CAGTACTGGT 
GGAATAGTCT TTGGCCAGCA ATCATCCTCT TCCAGTGGTA GCGTGTTTGG GTCTGGAAAC 
ACTGGAAGAG GGGGAGGTTT CTTCAGTGGC CTTGGAGGAA AACCCAGTCA GGATGCAGCC 
AACAAAAACC CATTCAGCTC GGCCAGTGGG GGCTTTGGAT CCACAGCTAC CTCAAATACC 
TCTAACCTAT TTGGAAACAG TGGGGCCAAG ACATTTGGTG GATTTGCCAG CTCGTCGTTT 
GGAGAGCAGA AACCCACTGG CACTTTCAGC TCTGGAGGAG GAAGTGTGGC ATCCCAAGGC 
TTTGGGTTTT CCTCTCCAAA CAAAACAGGT GGCTTCGGTG CTGCTCCAGT GTTTGGCAGC 
CCTCCTACTT TTGGGGGATC CCCTGGGTTT GGAGGGGTGC CAGCATTCGG TTCAGCCCCA 
GCCTTTACAA GCCCTCTGGG CTCGACGGGA GGCAAAGTGT TCGGAGAGGG CACTGCAGCT 
GCCAGCGCAG GAGGATTCGG GTTTGGGAGC AGCAGCAACA CCACATCCTT CGGCACGCTC 
GCGAGTCAGA ATGCCCCCAC TTTCGGATCA CTGTCCCAAC AGACTTCTGG TTTTGGGACC 
CAGAGTAGCG GATTCTCTGG TTTTGGATCA GGCACAGGAG GGTTCAGCTT TGGGTCAAAT 
AACTCGTCTG TCCAGGGTTT TGGTGGCTGG CGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272273.1
CDS	22..6420
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_005272216.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272216.3

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGACAGCCAA GATAGAAACA 
GCTGTGACTT CAACCCCATC TGCTTCTGGG CAGTTCAGCA AGCCTTTCTC ATTTTCTCCA 
TCAGGGACTG GCTTTAATTT TGGGATAATC ACACCAACAC CGTCTTCTAA TTTCACTGCT 
GCACAAGGGG CAACACCCTC CACTAAAGAG TCAAGCCAGC CGGACGCATT CTCATCTGGT 
GGGGGAAGCA AACCTTCTTA TGAGGCCATT CCTGAAAGCT CACCTCCCTC AGGAATCACA 
TCCGCATCAA ACACCACCCC AGGAGAACCT GCCGCATCTA GCAGCAGACC TGTGGCACCT 
TCTGGAACTG CTCTTTCCAC CACCTCTAGT AAGCTGGAAA CCCCACCGTC CAAGCTGGGA 
GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA GAGACTCTGG GAAGTTTTTC AGGACTGCGG 
GTTGGCCAAG CAGATGATTC TACAAAACCA ACCAATAAGG CTTCATCCAC AAGCCTAACT 
AGTACCCAGC CAACCAAGAC GTCAGGCGTG CCCTCAGGGT TTAATTTTAC TGCCCCCCCG 
GTGTTAGGGA AGCACACGGA GCCCCCTGTG ACATCCTCTG CAACCACCAC CTCAGTAGCA 
CCACCAGCAG CCACCAGCAC TTCCTCAACT GCCGTTTTTG GCAGTCTGCC AGTCACCAGT 
GCAGGATCCT CTGGGGTCAT CAGTTTTGGT GGGACATCTC TAAGTGCTGG CAAGACTAGT 
TTTTCATTTG GAAGCCAACA GACCAATAGC ACAGTGCCCC CATCTGCCCC ACCACCAACT 
ACAGCTGCCA CTCCCCTTCC AACATCATTC CCCACATTGT CATTTGGTAG CCTCCTGAGT 
TCAGCAACTA CCCCCTCCCT GCCTATGTCC GCTGGCAGAA GCACAGAAGA GGCCACTTCA 
TCAGCTTTGC CTGAGAAGCC AGGTGACAGT GAGGTCTCAG CATCAGCAGC CTCACTTCTA 
GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG GCTCCTCCGC AAACTTCTGA CTCTGTTAAA 
AAAGAACCTG TTCTTGCCCA GCCTGCAGTC AGCAACTCTG GCACTGCAGC ATCTAGTACT 
AGTCTTGTAG CACTTTCTGC AGAGGCTACC CCAGCCACCA CGGGGGTCCC TGATGCCAGG 
ACGGAGGCAG TACCACCTGC TTCCTCCTTT TCTGTGCCTG GGCAGACTGC TGTCACAGCA 
GCTGCTATCT CAAGTGCAGG CCCTGTGGCC GTCGAAACAT CAAGTACCCC CATAGCCTCC 
AGCACCACGT CCATTGTTGC TCCCGGCCCA TCTGCAGAGG CAGCAGCATT TGGTACCGTC 
ACTTCTGGCT CATCCGTCTT TGCTCAGCCT CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC 
CAGCTCACCA ACAACACAGC CACTGCCCCC TCTGCCACGC CCGTGTTTGG GCAAGTGGCA 
GCCAGCACCG CACCAAGTCT GTTTGGGCAG CAGACTGGTA GCACAGCCAG CACAGCAGCT 
GCCACACCAC AGGTCAGCAG CTCAGGGTTT AGCAGCCCAG CTTTTGGTAC CACAGCCCCA 
GGGGTCTTTG GACAGACAAC CTTCGGGCAG GCCTCAGTCT TTGGGCAGTC GGCGAGCAGT 
GCTGCAAGTG TCTTTTCCTT CAGTCAGCCT GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG 
CCTGCTTCCT CCACTCCCAC ATCCACCAGT GGAAGTGTCT TTGGTGCCGC CTCAAGTACC 
AGTAGCTCCA GTTCCTTCTC ATTTGGACAG TCTTCTCCCA ACACAGGAGG GGGGCTGTTT 
GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG AGTCCTGGCT TTGGACAGGG AGGCTCTGTC 
TTTGGTGGTA CCTCAGCTGC CACCACAACA GCAGCAACCT CTGGGTTCAG CTTTTGCCAA 
GCTTCAGGTT TTGGGTCTAG TAATACTGGT TCTGTGTTTG GTCAAGCAGC CAGTACTGGT 
GGAATAGTCT TTGGCCAGCA ATCATCCTCT TCCAGTGGTA GCGTGTTTGG GTCTGGAAAC 
ACTGGAAGAG GGGGAGGTTT CTTCAGTGGC CTTGGAGGAA AACCCAGTCA GGATGCAGCC 
AACAAAAACC CATTCAGCTC GGCCAGTGGG GGCTTTGGAT CCACAGCTAC CTCAAATACC 
TCTAACCTAT TTGGAAACAG TGGGGCCAAG ACATTTGGTG GATTTGCCAG CTCGTCGTTT 
GGAGAGCAGA AACCCACTGG CACTTTCAGC TCTGGAGGAG GAAGTGTGGC ATCCCAAGGC 
TTTGGGTTTT CCTCTCCAAA CAAAACAGGT GGCTTCGGTG CTGCTCCAGT GTTTGGCAGC 
CCTCCTACTT TTGGGGGATC CCCTGGGTTT GGAGGGGTGC CAGCATTCGG TTCAGCCCCA 
GCCTTTACAA GCCCTCTGGG CTCGACGGGA GGCAAAGTGT TCGGAGAGGG CACTGCAGCT 
GCCAGCGCAG GAGGATTCGG GTTTGGGAGC AGCAGCAACA CCACATCCTT CGGCACGCTC 
GCGAGTCAGA ATGCCCCCAC TTTCGGATCA CTGTCCCAAC AGACTTCTGG TTTTGGGACC 
CAGAGTAGCG GATTCTCTGG TTTTGGATCA GGCACAGGAG GGTTCAGCTT TGGGTCAAAT 
AACTCGTCTG TCCAGGGTTT TGGTGGCTGG CGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_006717292.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717355.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

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CloneID	OHu43526
Clone ID Related Accession (Same CDS sequence)	XM_006717292.3
Accession Version	XM_006717292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AATAGCTCCC AGAGCGCACC CCCGATGTCG 
CCATTCTCTT CTGCCTCCAA GCCAGCTGCT TCTGGACCAC TCAGCCACCC CACACCTCTC 
TCAGCACCAC CTAGTTCCGT GCCATTGAAG TCCTCAGTCT TGCCCTCACC ATCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717355.1
CDS	22..6384
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSENSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_006717292.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717292.3

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AATAGCTCCC AGAGCGCACC CCCGATGTCG 
CCATTCTCTT CTGCCTCCAA GCCAGCTGCT TCTGGACCAC TCAGCCACCC CACACCTCTC 
TCAGCACCAC CTAGTTCCGT GCCATTGAAG TCCTCAGTCT TGCCCTCACC ATCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_005272218.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272275.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X5, mRNA.

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CloneID	OHu43527
Clone ID Related Accession (Same CDS sequence)	XM_005272218.3
Accession Version	XM_005272218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGGCAACACC CTCCACTAAA 
GAGTCAAGCC AGCCGGACGC ATTCTCATCT GGTGGGGGAA GCAAACCTTC TTATGAGGCC 
ATTCCTGAAA GCTCACCTCC CTCAGGAATC ACATCCGCAT CAAACACCAC CCCAGGAGAA 
CCTGCCGCAT CTAGCAGCAG ACCTGTGGCA CCTTCTGGAA CTGCTCTTTC CACCACCTCT 
AGTAAGCTGG AAACCCCACC GTCCAAGCTG GGAGAGCTTC TGTTTCCAAG TTCTTTGGCT 
GGAGAGACTC TGGGAAGTTT TTCAGGACTG CGGGTTGGCC AAGCAGATGA TTCTACAAAA 
CCAACCAATA AGGCTTCATC CACAAGCCTA ACTAGTACCC AGCCAACCAA GACGTCAGGC 
GTGCCCTCAG GGTTTAATTT TACTGCCCCC CCGGTGTTAG GGAAGCACAC GGAGCCCCCT 
GTGACATCCT CTGCAACCAC CACCTCAGTA GCACCACCAG CAGCCACCAG CACTTCCTCA 
ACTGCCGTTT TTGGCAGTCT GCCAGTCACC AGTGCAGGAT CCTCTGGGGT CATCAGTTTT 
GGTGGGACAT CTCTAAGTGC TGGCAAGACT AGTTTTTCAT TTGGAAGCCA ACAGACCAAT 
AGCACAGTGC CCCCATCTGC CCCACCACCA ACTACAGCTG CCACTCCCCT TCCAACATCA 
TTCCCCACAT TGTCATTTGG TAGCCTCCTG AGTTCAGCAA CTACCCCCTC CCTGCCTATG 
TCCGCTGGCA GAAGCACAGA AGAGGCCACT TCATCAGCTT TGCCTGAGAA GCCAGGTGAC 
AGTGAGGTCT CAGCATCAGC AGCCTCACTT CTAGAGGAGC AACAGTCAGC CCAGCTTCCC 
CAGGCTCCTC CGCAAACTTC TGACTCTGTT AAAAAAGAAC CTGTTCTTGC CCAGCCTGCA 
GTCAGCAACT CTGGCACTGC AGCATCTAGT ACTAGTCTTG TAGCACTTTC TGCAGAGGCT 
ACCCCAGCCA CCACGGGGGT CCCTGATGCC AGGACGGAGG CAGTACCACC TGCTTCCTCC 
TTTTCTGTGC CTGGGCAGAC TGCTGTCACA GCAGCTGCTA TCTCAAGTGC AGGCCCTGTG 
GCCGTCGAAA CATCAAGTAC CCCCATAGCC TCCAGCACCA CGTCCATTGT TGCTCCCGGC 
CCATCTGCAG AGGCAGCAGC ATTTGGTACC GTCACTTCTG GCTCATCCGT CTTTGCTCAG 
CCTCCTGCTG CCAGTTCTAG CTCAGCTTTC AACCAGCTCA CCAACAACAC AGCCACTGCC 
CCCTCTGCCA CGCCCGTGTT TGGGCAAGTG GCAGCCAGCA CCGCACCAAG TCTGTTTGGG 
CAGCAGACTG GTAGCACAGC CAGCACAGCA GCTGCCACAC CACAGGTCAG CAGCTCAGGG 
TTTAGCAGCC CAGCTTTTGG TACCACAGCC CCAGGGGTCT TTGGACAGAC AACCTTCGGG 
CAGGCCTCAG TCTTTGGGCA GTCGGCGAGC AGTGCTGCAA GTGTCTTTTC CTTCAGTCAG 
CCTGGGTTCA GTTCCGTGCC TGCCTTCGGT CAGCCTGCTT CCTCCACTCC CACATCCACC 
AGTGGAAGTG TCTTTGGTGC CGCCTCAAGT ACCAGTAGCT CCAGTTCCTT CTCATTTGGA 
CAGTCTTCTC CCAACACAGG AGGGGGGCTG TTTGGCCAAA GCAACGCTCC TGCTTTTGGG 
CAGAGTCCTG GCTTTGGACA GGGAGGCTCT GTCTTTGGTG GTACCTCAGC TGCCACCACA 
ACAGCAGCAA CCTCTGGGTT CAGCTTTTGC CAAGCTTCAG GTTTTGGGTC TAGTAATACT 
GGTTCTGTGT TTGGTCAAGC AGCCAGTACT GGTGGAATAG TCTTTGGCCA GCAATCATCC 
TCTTCCAGTG GTAGCGTGTT TGGGTCTGGA AACACTGGAA GAGGGGGAGG TTTCTTCAGT 
GGCCTTGGAG GAAAACCCAG TCAGGATGCA GCCAACAAAA ACCCATTCAG CTCGGCCAGT 
GGGGGCTTTG GATCCACAGC TACCTCAAAT ACCTCTAACC TATTTGGAAA CAGTGGGGCC 
AAGACATTTG GTGGATTTGC CAGCTCGTCG TTTGGAGAGC AGAAACCCAC TGGCACTTTC 
AGCTCTGGAG GAGGAAGTGT GGCATCCCAA GGCTTTGGGT TTTCCTCTCC AAACAAAACA 
GGTGGCTTCG GTGCTGCTCC AGTGTTTGGC AGCCCTCCTA CTTTTGGGGG ATCCCCTGGG 
TTTGGAGGGG TGCCAGCATT CGGTTCAGCC CCAGCCTTTA CAAGCCCTCT GGGCTCGACG 
GGAGGCAAAG TGTTCGGAGA GGGCACTGCA GCTGCCAGCG CAGGAGGATT CGGGTTTGGG 
AGCAGCAGCA ACACCACATC CTTCGGCACG CTCGCGAGTC AGAATGCCCC CACTTTCGGA 
TCACTGTCCC AACAGACTTC TGGTTTTGGG ACCCAGAGTA GCGGATTCTC TGGTTTTGGA 
TCAGGCACAG GAGGGTTCAG CTTTGGGTCA AATAACTCGT CTGTCCAGGG TTTTGGTGGC 
TGGCGAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272275.1
CDS	22..6273
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_005272218.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272218.3

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AAGTTTACTG CTGCAGCTAC CTCTACTCCT 
GTTAGTAGCT CCCAGAGCGC ACCCCCGATG TCGCCATTCT CTTCTGCCTC CAAGCCAGCT 
GCTTCTGGAC CACTCAGCCA CCCCACACCT CTCTCAGCAC CACCTAGTTC CGTGCCATTG 
AAGTCCTCAG TCTTGCCCTC ACCATCAGCA GGACGATCTG CTCAGGGCAG TTCAAGCCCA 
GTGCCCTCAA TGGTACAGAA ATCACCCAGG ATAACCCCTC CAGCGGCAAA GCCAGGCTCT 
CCCCAGGCAA AGTCACTTCA GCCTGCTGTT GCAGAAAAGC AGGGACATCA GTGGAAAGAT 
TCAGATCCTG TAATGGCTGG AATTGGGGAG GAGATTGCAC ACTTTCAGAA GGAGTTGGAA 
GAGTTAAAAG CCCGAACTTC CAAAGCCTGT TTCCAAGTGG GCACTTCTGA GGAGATGAAG 
ATGCTGCGAA CAGAATCAGA TGACTTGCAT ACCTTTCTTT TGGAGATTAA AGAGACCACA 
GAGTCGCTTC ATGGAGATAT AAGTAGCCTG AAAACAACTT TACTTGAGGG CTTTGCTGGT 
GTTGAGGAAG CCAGAGAACA AAATGAAAGA AATCGTGACT CTGGTTATCT GCATTTGCTT 
TATAAAAGAC CACTGGATCC CAAGAGTGAA GCTCAGCTTC AGGAAATTCG GCGCCTTCAT 
CAGTATGTGA AATTTGCTGT CCAAGATGTG AATGATGTTC TAGACTTGGA GTGGGATCAG 
CATCTGGAAC AAAAGAAAAA ACAAAGGCAC CTGCTTGTGC CAGAGCGAGA GACACTGTTT 
AACACCCTAG CCAACAATCG GGAAATCATC AACCAACAGA GGAAGAGGCT GAATCACCTG 
GTGGATAGTC TTCAGCAGCT CCGCCTTTAC AAACAGACTT CCCTGTGGAG CCTGTCCTCG 
GCTGTTCCTT CCCAGAGCAG CATTCACAGT TTTGACAGTG ACCTGGAAAG CCTGTGCAAT 
GCTTTGTTGA AAACCACCAT AGAATCTCAC ACCAAATCCT TGCCCAAAGT ACCAGCCAAA 
CTGTCCCCCA TGAAACAGGC ACAACTGAGA AACTTCTTGG CCAAGAGGAA GACCCCACCA 
GTGAGATCCA CTGCTCCAGC CAGCCTGTCT CGATCAGCCT TTCTGTCTCA GAGATATTAT 
GAAGACTTGG ATGAAGTCAG CTCAACGTCA TCTGTCTCCC AGTCTCTGGA GAGTGAAGAT 
GCACGGACGT CCTGTAAAGA TGACGAGGCA GTGGTTCAGG CCCCTCGGCA CGCCCCCGTG 
GTTCGCACTC CTTCCATCCA GCCCAGTCTC TTGCCCCATG CAGCACCTTT TGCTAAATCT 
CACCTGGTTC ATGGTTCTTC ACCTGGTGTG ATGGGAACTT CAGTGGCTAC ATCTGCTAGC 
AAAATTATTC CTCAAGGGGC CGATAGCACA ATGCTTGCCA CGAAAACCGT GAAACATGGT 
GCACCTAGTC CTTCCCACCC CATCTCAGCC CCGCAGGCAG CTGCCGCAGC AGCACTCAGG 
CGGCAGATGG CCAGTCAGGC ACCAGCTGTA AACACTTTGA CTGAATCAAC GTTGAAGAAT 
GTCCCTCAAG TGGTAAATGT GCAGGAATTG AAGAATAACC CTGCAACCCC TTCTACAGCC 
ATGGGTTCTT CAGTGCCCTA CTCCACAGCC AAAACACCTC ACCCAGTGTT GACCCCAGTG 
GCTGCTAACC AAGCCAAGCA GGGGTCTCTA ATAAATTCCC TTAAGCCATC TGGGCCTACA 
CCAGCATCCG GTCAGTTATC ATCTGGTGAC AAAGCTTCAG GGGCAACACC CTCCACTAAA 
GAGTCAAGCC AGCCGGACGC ATTCTCATCT GGTGGGGGAA GCAAACCTTC TTATGAGGCC 
ATTCCTGAAA GCTCACCTCC CTCAGGAATC ACATCCGCAT CAAACACCAC CCCAGGAGAA 
CCTGCCGCAT CTAGCAGCAG ACCTGTGGCA CCTTCTGGAA CTGCTCTTTC CACCACCTCT 
AGTAAGCTGG AAACCCCACC GTCCAAGCTG GGAGAGCTTC TGTTTCCAAG TTCTTTGGCT 
GGAGAGACTC TGGGAAGTTT TTCAGGACTG CGGGTTGGCC AAGCAGATGA TTCTACAAAA 
CCAACCAATA AGGCTTCATC CACAAGCCTA ACTAGTACCC AGCCAACCAA GACGTCAGGC 
GTGCCCTCAG GGTTTAATTT TACTGCCCCC CCGGTGTTAG GGAAGCACAC GGAGCCCCCT 
GTGACATCCT CTGCAACCAC CACCTCAGTA GCACCACCAG CAGCCACCAG CACTTCCTCA 
ACTGCCGTTT TTGGCAGTCT GCCAGTCACC AGTGCAGGAT CCTCTGGGGT CATCAGTTTT 
GGTGGGACAT CTCTAAGTGC TGGCAAGACT AGTTTTTCAT TTGGAAGCCA ACAGACCAAT 
AGCACAGTGC CCCCATCTGC CCCACCACCA ACTACAGCTG CCACTCCCCT TCCAACATCA 
TTCCCCACAT TGTCATTTGG TAGCCTCCTG AGTTCAGCAA CTACCCCCTC CCTGCCTATG 
TCCGCTGGCA GAAGCACAGA AGAGGCCACT TCATCAGCTT TGCCTGAGAA GCCAGGTGAC 
AGTGAGGTCT CAGCATCAGC AGCCTCACTT CTAGAGGAGC AACAGTCAGC CCAGCTTCCC 
CAGGCTCCTC CGCAAACTTC TGACTCTGTT AAAAAAGAAC CTGTTCTTGC CCAGCCTGCA 
GTCAGCAACT CTGGCACTGC AGCATCTAGT ACTAGTCTTG TAGCACTTTC TGCAGAGGCT 
ACCCCAGCCA CCACGGGGGT CCCTGATGCC AGGACGGAGG CAGTACCACC TGCTTCCTCC 
TTTTCTGTGC CTGGGCAGAC TGCTGTCACA GCAGCTGCTA TCTCAAGTGC AGGCCCTGTG 
GCCGTCGAAA CATCAAGTAC CCCCATAGCC TCCAGCACCA CGTCCATTGT TGCTCCCGGC 
CCATCTGCAG AGGCAGCAGC ATTTGGTACC GTCACTTCTG GCTCATCCGT CTTTGCTCAG 
CCTCCTGCTG CCAGTTCTAG CTCAGCTTTC AACCAGCTCA CCAACAACAC AGCCACTGCC 
CCCTCTGCCA CGCCCGTGTT TGGGCAAGTG GCAGCCAGCA CCGCACCAAG TCTGTTTGGG 
CAGCAGACTG GTAGCACAGC CAGCACAGCA GCTGCCACAC CACAGGTCAG CAGCTCAGGG 
TTTAGCAGCC CAGCTTTTGG TACCACAGCC CCAGGGGTCT TTGGACAGAC AACCTTCGGG 
CAGGCCTCAG TCTTTGGGCA GTCGGCGAGC AGTGCTGCAA GTGTCTTTTC CTTCAGTCAG 
CCTGGGTTCA GTTCCGTGCC TGCCTTCGGT CAGCCTGCTT CCTCCACTCC CACATCCACC 
AGTGGAAGTG TCTTTGGTGC CGCCTCAAGT ACCAGTAGCT CCAGTTCCTT CTCATTTGGA 
CAGTCTTCTC CCAACACAGG AGGGGGGCTG TTTGGCCAAA GCAACGCTCC TGCTTTTGGG 
CAGAGTCCTG GCTTTGGACA GGGAGGCTCT GTCTTTGGTG GTACCTCAGC TGCCACCACA 
ACAGCAGCAA CCTCTGGGTT CAGCTTTTGC CAAGCTTCAG GTTTTGGGTC TAGTAATACT 
GGTTCTGTGT TTGGTCAAGC AGCCAGTACT GGTGGAATAG TCTTTGGCCA GCAATCATCC 
TCTTCCAGTG GTAGCGTGTT TGGGTCTGGA AACACTGGAA GAGGGGGAGG TTTCTTCAGT 
GGCCTTGGAG GAAAACCCAG TCAGGATGCA GCCAACAAAA ACCCATTCAG CTCGGCCAGT 
GGGGGCTTTG GATCCACAGC TACCTCAAAT ACCTCTAACC TATTTGGAAA CAGTGGGGCC 
AAGACATTTG GTGGATTTGC CAGCTCGTCG TTTGGAGAGC AGAAACCCAC TGGCACTTTC 
AGCTCTGGAG GAGGAAGTGT GGCATCCCAA GGCTTTGGGT TTTCCTCTCC AAACAAAACA 
GGTGGCTTCG GTGCTGCTCC AGTGTTTGGC AGCCCTCCTA CTTTTGGGGG ATCCCCTGGG 
TTTGGAGGGG TGCCAGCATT CGGTTCAGCC CCAGCCTTTA CAAGCCCTCT GGGCTCGACG 
GGAGGCAAAG TGTTCGGAGA GGGCACTGCA GCTGCCAGCG CAGGAGGATT CGGGTTTGGG 
AGCAGCAGCA ACACCACATC CTTCGGCACG CTCGCGAGTC AGAATGCCCC CACTTTCGGA 
TCACTGTCCC AACAGACTTC TGGTTTTGGG ACCCAGAGTA GCGGATTCTC TGGTTTTGGA 
TCAGGCACAG GAGGGTTCAG CTTTGGGTCA AATAACTCGT CTGTCCAGGG TTTTGGTGGC 
TGGCGAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_005272219.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272276.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X6, mRNA.

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CloneID	OHu43528
Clone ID Related Accession (Same CDS sequence)	XM_005272219.3
Accession Version	XM_005272219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGCCT CCCTAGCCCC CACCCCTGCA 
GCGTCTCCTG TGGCTCCATC AGCTGCTTCA TTCTCCTTTG GATCATCTGG TTTTAAGCCT 
ACCCTGGAAA GCACACCAGT GCCAAGTGTG TCTGCTCCAA ATATAGCAAT GAAGCCCTCC 
TTCCCACCCT CAACCTCTGC TGTCAAAGTC AACCTTAGTG AAAAGTTTAC TGCTGCAGCT 
ACCTCTACTC CTGTTAGTAG CTCCCAGAGC GCACCCCCGA TGTCGCCATT CTCTTCTGCC 
TCCAAGCCAG CTGCTTCTGG ACCACTCAGC CACCCCACAC CTCTCTCAGC ACCACCTAGT 
TCCGTGCCAT TGAAGTCCTC AGTCTTGCCC TCACCATCAG CAGGACGATC TGCTCAGGGC 
AGTTCAAGCC CAGTGCCCTC AATGGTACAG AAATCACCCA GGATAACCCC TCCAGCGGCA 
AAGCCAGGCT CTCCCCAGGC AAAGTCACTT CAGCCTGCTG TTGCAGAAAA GCAGGGACAT 
CAGTGGAAAG ATTCAGATCC TGTAATGGCT GGAATTGGGG AGGAGATTGC ACACTTTCAG 
AAGGAGTTGG AAGAGTTAAA AGCCCGAACT TCCAAAGCCT GTTTCCAAGT GGGCACTTCT 
GAGGAGATGA AGATGCTGCG AACAGAATCA GATGACTTGC ATACCTTTCT TTTGGAGATT 
AAAGAGACCA CAGAGTCGCT TCATGGAGAT ATAAGTAGCC TGAAAACAAC TTTACTTGAG 
GGCTTTGCTG GTGTTGAGGA AGCCAGAGAA CAAAATGAAA GAAATCGTGA CTCTGGTTAT 
CTGCATTTGC TTTATAAAAG ACCACTGGAT CCCAAGAGTG AAGCTCAGCT TCAGGAAATT 
CGGCGCCTTC ATCAGTATGT GAAATTTGCT GTCCAAGATG TGAATGATGT TCTAGACTTG 
GAGTGGGATC AGCATCTGGA ACAAAAGAAA AAACAAAGGC ACCTGCTTGT GCCAGAGCGA 
GAGACACTGT TTAACACCCT AGCCAACAAT CGGGAAATCA TCAACCAACA GAGGAAGAGG 
CTGAATCACC TGGTGGATAG TCTTCAGCAG CTCCGCCTTT ACAAACAGAC TTCCCTGTGG 
AGCCTGTCCT CGGCTGTTCC TTCCCAGAGC AGCATTCACA GTTTTGACAG TGACCTGGAA 
AGCCTGTGCA ATGCTTTGTT GAAAACCACC ATAGAATCTC ACACCAAATC CTTGCCCAAA 
GTACCAGCCA AACTGTCCCC CATGAAACAG GCACAACTGA GAAACTTCTT GGCCAAGAGG 
AAGACCCCAC CAGTGAGATC CACTGCTCCA GCCAGCCTGT CTCGATCAGC CTTTCTGTCT 
CAGAGATATT ATGAAGACTT GGATGAAGTC AGCTCAACGT CATCTGTCTC CCAGTCTCTG 
GAGAGTGAAG ATGCACGGAC GTCCTGTAAA GATGACGAGG CAGTGGTTCA GGCCCCTCGG 
CACGCCCCCG TGGTTCGCAC TCCTTCCATC CAGCCCAGTC TCTTGCCCCA TGCAGCACCT 
TTTGCTAAAT CTCACCTGGT TCATGGTTCT TCACCTGGTG TGATGGGAAC TTCAGTGGCT 
ACATCTGCTA GCAAAATTAT TCCTCAAGGG GCCGATAGCA CAATGCTTGC CACGAAAACC 
GTGAAACATG GTGCACCTAG TCCTTCCCAC CCCATCTCAG CCCCGCAGGC AGCTGCCGCA 
GCAGCACTCA GGCGGCAGAT GGCCAGTCAG GCACCAGCTG TAAACACTTT GACTGAATCA 
ACGTTGAAGA ATGTCCCTCA AGTGGTAAAT GTGCAGGAAT TGAAGAATAA CCCTGCAACC 
CCTTCTACAG CCATGGGTTC TTCAGTGCCC TACTCCACAG CCAAAACACC TCACCCAGTG 
TTGACCCCAG TGGCTGCTAA CCAAGCCAAG CAGGGGTCTC TAATAAATTC CCTTAAGCCA 
TCTGGGCCTA CACCAGCATC CGGTCAGTTA TCATCTGGTG ACAAAGCTTC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272276.1
CDS	22..6132
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_005272219.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272219.3

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGCCT CCCTAGCCCC CACCCCTGCA 
GCGTCTCCTG TGGCTCCATC AGCTGCTTCA TTCTCCTTTG GATCATCTGG TTTTAAGCCT 
ACCCTGGAAA GCACACCAGT GCCAAGTGTG TCTGCTCCAA ATATAGCAAT GAAGCCCTCC 
TTCCCACCCT CAACCTCTGC TGTCAAAGTC AACCTTAGTG AAAAGTTTAC TGCTGCAGCT 
ACCTCTACTC CTGTTAGTAG CTCCCAGAGC GCACCCCCGA TGTCGCCATT CTCTTCTGCC 
TCCAAGCCAG CTGCTTCTGG ACCACTCAGC CACCCCACAC CTCTCTCAGC ACCACCTAGT 
TCCGTGCCAT TGAAGTCCTC AGTCTTGCCC TCACCATCAG CAGGACGATC TGCTCAGGGC 
AGTTCAAGCC CAGTGCCCTC AATGGTACAG AAATCACCCA GGATAACCCC TCCAGCGGCA 
AAGCCAGGCT CTCCCCAGGC AAAGTCACTT CAGCCTGCTG TTGCAGAAAA GCAGGGACAT 
CAGTGGAAAG ATTCAGATCC TGTAATGGCT GGAATTGGGG AGGAGATTGC ACACTTTCAG 
AAGGAGTTGG AAGAGTTAAA AGCCCGAACT TCCAAAGCCT GTTTCCAAGT GGGCACTTCT 
GAGGAGATGA AGATGCTGCG AACAGAATCA GATGACTTGC ATACCTTTCT TTTGGAGATT 
AAAGAGACCA CAGAGTCGCT TCATGGAGAT ATAAGTAGCC TGAAAACAAC TTTACTTGAG 
GGCTTTGCTG GTGTTGAGGA AGCCAGAGAA CAAAATGAAA GAAATCGTGA CTCTGGTTAT 
CTGCATTTGC TTTATAAAAG ACCACTGGAT CCCAAGAGTG AAGCTCAGCT TCAGGAAATT 
CGGCGCCTTC ATCAGTATGT GAAATTTGCT GTCCAAGATG TGAATGATGT TCTAGACTTG 
GAGTGGGATC AGCATCTGGA ACAAAAGAAA AAACAAAGGC ACCTGCTTGT GCCAGAGCGA 
GAGACACTGT TTAACACCCT AGCCAACAAT CGGGAAATCA TCAACCAACA GAGGAAGAGG 
CTGAATCACC TGGTGGATAG TCTTCAGCAG CTCCGCCTTT ACAAACAGAC TTCCCTGTGG 
AGCCTGTCCT CGGCTGTTCC TTCCCAGAGC AGCATTCACA GTTTTGACAG TGACCTGGAA 
AGCCTGTGCA ATGCTTTGTT GAAAACCACC ATAGAATCTC ACACCAAATC CTTGCCCAAA 
GTACCAGCCA AACTGTCCCC CATGAAACAG GCACAACTGA GAAACTTCTT GGCCAAGAGG 
AAGACCCCAC CAGTGAGATC CACTGCTCCA GCCAGCCTGT CTCGATCAGC CTTTCTGTCT 
CAGAGATATT ATGAAGACTT GGATGAAGTC AGCTCAACGT CATCTGTCTC CCAGTCTCTG 
GAGAGTGAAG ATGCACGGAC GTCCTGTAAA GATGACGAGG CAGTGGTTCA GGCCCCTCGG 
CACGCCCCCG TGGTTCGCAC TCCTTCCATC CAGCCCAGTC TCTTGCCCCA TGCAGCACCT 
TTTGCTAAAT CTCACCTGGT TCATGGTTCT TCACCTGGTG TGATGGGAAC TTCAGTGGCT 
ACATCTGCTA GCAAAATTAT TCCTCAAGGG GCCGATAGCA CAATGCTTGC CACGAAAACC 
GTGAAACATG GTGCACCTAG TCCTTCCCAC CCCATCTCAG CCCCGCAGGC AGCTGCCGCA 
GCAGCACTCA GGCGGCAGAT GGCCAGTCAG GCACCAGCTG TAAACACTTT GACTGAATCA 
ACGTTGAAGA ATGTCCCTCA AGTGGTAAAT GTGCAGGAAT TGAAGAATAA CCCTGCAACC 
CCTTCTACAG CCATGGGTTC TTCAGTGCCC TACTCCACAG CCAAAACACC TCACCCAGTG 
TTGACCCCAG TGGCTGCTAA CCAAGCCAAG CAGGGGTCTC TAATAAATTC CCTTAAGCCA 
TCTGGGCCTA CACCAGCATC CGGTCAGTTA TCATCTGGTG ACAAAGCTTC AGGGACAGCC 
AAGATAGAAA CAGCTGTGAC TTCAACCCCA TCTGCTTCTG GGCAGTTCAG CAAGCCTTTC 
TCATTTTCTC CATCAGGGAC TGGCTTTAAT TTTGGGATAA TCACACCAAC ACCGTCTTCT 
AATTTCACTG CTGCACAAGG GGCAACACCC TCCACTAAAG AGTCAAGCCA GCCGGACGCA 
TTCTCATCTG GTGGGGGAAG CAAACCTTCT TATGAGGCCA TTCCTGAAAG CTCACCTCCC 
TCAGGAATCA CATCCGCATC AAACACCACC CCAGGAGAAC CTGCCGCATC TAGCAGCAGA 
CCTGTGGCAC CTTCTGGAAC TGCTCTTTCC ACCACCTCTA GTAAGCTGGA AACCCCACCG 
TCCAAGCTGG GAGAGCTTCT GTTTCCAAGT TCTTTGGCTG GAGAGACTCT GGGAAGTTTT 
TCAGGACTGC GGGTTGGCCA AGCAGATGAT TCTACAAAAC CAACCAATAA GGCTTCATCC 
ACAAGCCTAA CTAGTACCCA GCCAACCAAG ACGTCAGGCG TGCCCTCAGG GTTTAATTTT 
ACTGCCCCCC CGGTGTTAGG GAAGCACACG GAGCCCCCTG TGACATCCTC TGCAACCACC 
ACCTCAGTAG CACCACCAGC AGCCACCAGC ACTTCCTCAA CTGCCGTTTT TGGCAGTCTG 
CCAGTCACCA GTGCAGGATC CTCTGGGGTC ATCAGTTTTG GTGGGACATC TCTAAGTGCT 
GGCAAGACTA GTTTTTCATT TGGAAGCCAA CAGACCAATA GCACAGTGCC CCCATCTGCC 
CCACCACCAA CTACAGCTGC CACTCCCCTT CCAACATCAT TCCCCACATT GTCATTTGGT 
AGCCTCCTGA GTTCAGCAAC TACCCCCTCC CTGCCTATGT CCGCTGGCAG AAGCACAGAA 
GAGGCCACTT CATCAGCTTT GCCTGAGAAG CCAGGTGACA GTGAGGTCTC AGCATCAGCA 
GCCTCACTTC TAGAGGAGCA ACAGTCAGCC CAGCTTCCCC AGGCTCCTCC GCAAACTTCT 
GACTCTGTTA AAAAAGAACC TGTTCTTGCC CAGCCTGCAG TCAGCAACTC TGGCACTGCA 
GCATCTAGTA CTAGTCTTGT AGCACTTTCT GCAGAGGCTA CCCCAGCCAC CACGGGGGTC 
CCTGATGCCA GGACGGAGGC AGTACCACCT GCTTCCTCCT TTTCTGTGCC TGGGCAGACT 
GCTGTCACAG CAGCTGCTAT CTCAAGTGCA GGCCCTGTGG CCGTCGAAAC ATCAAGTACC 
CCCATAGCCT CCAGCACCAC GTCCATTGTT GCTCCCGGCC CATCTGCAGA GGCAGCAGCA 
TTTGGTACCG TCACTTCTGG CTCATCCGTC TTTGCTCAGC CTCCTGCTGC CAGTTCTAGC 
TCAGCTTTCA ACCAGCTCAC CAACAACACA GCCACTGCCC CCTCTGCCAC GCCCGTGTTT 
GGGCAAGTGG CAGCCAGCAC CGCACCAAGT CTGTTTGGGC AGCAGACTGG TAGCACAGCC 
AGCACAGCAG CTGCCACACC ACAGGTCAGC AGCTCAGGGT TTAGCAGCCC AGCTTTTGGT 
ACCACAGCCC CAGGGGTCTT TGGACAGACA ACCTTCGGGC AGGCCTCAGT CTTTGGGCAG 
TCGGCGAGCA GTGCTGCAAG TGTCTTTTCC TTCAGTCAGC CTGGGTTCAG TTCCGTGCCT 
GCCTTCGGTC AGCCTGCTTC CTCCACTCCC ACATCCACCA GTGGAAGTGT CTTTGGTGCC 
GCCTCAAGTA CCAGTAGCTC CAGTTCCTTC TCATTTGGAC AGTCTTCTCC CAACACAGGA 
GGGGGGCTGT TTGGCCAAAG CAACGCTCCT GCTTTTGGGC AGAGTCCTGG CTTTGGACAG 
GGAGGCTCTG TCTTTGGTGG TACCTCAGCT GCCACCACAA CAGCAGCAAC CTCTGGGTTC 
AGCTTTTGCC AAGCTTCAGG TTTTGGGTCT AGTAATACTG GTTCTGTGTT TGGTCAAGCA 
GCCAGTACTG GTGGAATAGT CTTTGGCCAG CAATCATCCT CTTCCAGTGG TAGCGTGTTT 
GGGTCTGGAA ACACTGGAAG AGGGGGAGGT TTCTTCAGTG GCCTTGGAGG AAAACCCAGT 
CAGGATGCAG CCAACAAAAA CCCATTCAGC TCGGCCAGTG GGGGCTTTGG ATCCACAGCT 
ACCTCAAATA CCTCTAACCT ATTTGGAAAC AGTGGGGCCA AGACATTTGG TGGATTTGCC 
AGCTCGTCGT TTGGAGAGCA GAAACCCACT GGCACTTTCA GCTCTGGAGG AGGAAGTGTG 
GCATCCCAAG GCTTTGGGTT TTCCTCTCCA AACAAAACAG GTGGCTTCGG TGCTGCTCCA 
GTGTTTGGCA GCCCTCCTAC TTTTGGGGGA TCCCCTGGGT TTGGAGGGGT GCCAGCATTC 
GGTTCAGCCC CAGCCTTTAC AAGCCCTCTG GGCTCGACGG GAGGCAAAGT GTTCGGAGAG 
GGCACTGCAG CTGCCAGCGC AGGAGGATTC GGGTTTGGGA GCAGCAGCAA CACCACATCC 
TTCGGCACGC TCGCGAGTCA GAATGCCCCC ACTTTCGGAT CACTGTCCCA ACAGACTTCT 
GGTTTTGGGA CCCAGAGTAG CGGATTCTCT GGTTTTGGAT CAGGCACAGG AGGGTTCAGC 
TTTGGGTCAA ATAACTCGTC TGTCCAGGGT TTTGGTGGCT GGCGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_011519053.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517355.1 Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

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CloneID	OHu61584
Clone ID Related Accession (Same CDS sequence)	XM_011519053.2
Accession Version	XM_011519053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AACTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517355.1
CDS	22..6384
Translation	MRGQLRAAGAEGRKFAVERPGFRGQGRGRQRWLLRHTEGGAMGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSENSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSAGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_011519053.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519053.2

ORF Insert Sequence:

ATGCGAGGTC AACTGCGCGC CGCTGGCGCT GAGGGGAGGA AGTTTGCTGT CGAGCGGCCT 
GGGTTCCGTG GGCAAGGCCG TGGGAGGCAG CGTTGGCTGC TTCGACACAC TGAGGGCGGC 
GCGATGGGAG ACGAGATGGA TGCCATGATT CCCGAGCGGG AGATGAAGGA TTTTCAGTTT 
AGAGCGCTAA AGAAGGTGAG AATCTTTGAC TCCCCTGAGG AATTGCCCAA GGAACGCTCG 
AGTCTGCTTG CTGTGTCCAA CAAATATGGT CTGGTCTTCG CTGGTGGAGC CAGTGGCTTG 
CAGATTTTTC CTACTAAAAA TCTTCTTATT CAAAATAAAC CCGGAGATGA TCCCAACAAA 
ATAGTTGATA AAGTCCAAGG CTTGCTAGTT CCTATGAAAT TCCCAATCCA TCACCTGGCC 
TTGAGCTGTG ATAACCTCAC ACTCTCTGCG TGCATGATGT CCAGTGAATA TGGTTCCATT 
ATTGCTTTTT TTGATGTTCG CACATTCTCA AATGAGGCTA AACAGCAAAA ACGCCCATTT 
GCCTATCATA AGCTTTTGAA AGATGCAGGA GGCATGGTGA TTGATATGAA GTGGAACCCC 
ACTGTCCCCT CCATGGTGGC AGTTTGTCTG GCTGATGGTA GTATTGCTGT CCTGCAAGTC 
ACGGAAACAG TGAAAGTATG TGCAACTCTT CCTTCCACGG TAGCAGTAAC CTCTGTGTGC 
TGGAGCCCCA AAGGAAAGCA GCTGGCAGTG GGAAAACAGA ATGGAACTGT GGTCCAGTAT 
CTTCCTACTT TGCAGGAAAA AAAAGTCATT CCTTGTCCTC CGTTTTATGA GTCAGATCAT 
CCTGTCAGAG TTCTGGATGT GCTGTGGATT GGTACCTACG TCTTCGCCAT AGTGTATGCT 
GCTGCAGATG GGACCCTGGA AACGTCTCCA GATGTGGTGA TGGCTCTACT ACCGAAAAAA 
GAAGAAAAGC ACCCAGAGAT ATTTGTGAAC TTTATGGAGC CCTGTTATGG CAGCTGCACG 
GAGAGACAGC ATCATTACTA CCTCAGTTAC ATTGAGGAAT GGGATTTAGT GCTGGCAGCA 
TCTGCGGCTT CAACAGAAGT TAGTATCCTT GCTCGACAAA GTGATCAGAT TAATTGGGAA 
TCTTGGCTAC TGGAGGATTC TAGTCGAGCT GAATTGCCTG TGACAGACAA GAGTGATGAC 
TCCTTGCCCA TGGGAGTTGT CGTAGACTAT ACAAACCAAG TGGAAATCAC CATCAGTGAT 
GAAAAGACTC TTCCTCCTGC TCCAGTTCTC ATGTTACTTT CAACAGATGG TGTGCTTTGT 
CCATTTTATA TGATTAATCA AAATCCTGGG GTTAAGTCTC TCATCAAAAC ACCAGAGCGA 
CTTTCATTAG AAGGAGAGCG ACAGCCCAAG TCACCAGGAA GTACTCCCAC TACCCCAACC 
TCCTCTCAAG CCCCACAGAA ACTGGATGCT TCTGCAGCTG CAGCCCCTGC CTCTCTGCCA 
CCTTCATCAC CTGCTGCTCC CATTGCCACT TTTTCTTTGC TTCCTGCTGG TGGAGCCCCC 
ACTGTGTTCT CCTTTGGTTC TTCATCTTTG AAGTCATCTG CTACGGTCAC TGGGGAGCCC 
CCTTCATATT CCAGTGGCTC CGACAGCTCC AAAGCAGCCC CAGGCCCTGG CCCATCAACC 
TTCTCTTTTG TTCCCCCTTC TAAAGCCTCC CTAGCCCCCA CCCCTGCAGC GTCTCCTGTG 
GCTCCATCAG CTGCTTCATT CTCCTTTGGA TCATCTGGTT TTAAGCCTAC CCTGGAAAGC 
ACACCAGTGC CAAGTGTGTC TGCTCCAAAT ATAGCAATGA AGCCCTCCTT CCCACCCTCA 
ACCTCTGCTG TCAAAGTCAA CCTTAGTGAA AACTCCCAGA GCGCACCCCC GATGTCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTGCTTCT GGACCACTCA GCCACCCCAC ACCTCTCTCA 
GCACCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTTCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGCGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAA 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ATATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGACT TGGAGTGGGA TCAGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ATCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAT AGTCTTCAGC AGCTCCGCCT TTACAAACAG 
ACTTCCCTGT GGAGCCTGTC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTGG AAAGCCTGTG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTGTA AAGATGACGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCA TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGC CCTACTCCAC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCTGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
TCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCAACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCCGGACG CATTCTCATC TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCGCA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACCACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGGGTTGGC CAAGCAGATG ATTCTACAAA ACCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCGGTGTTA GGGAAGCACA CGGAGCCCCC TGTGACATCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGCCGTT 
TTTGGCAGTC TGCCAGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG CTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCACC AACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCATTTG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCGCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGTTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAGCC 
ACCACGGGGG TCCCTGATGC CAGGACGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTA CCCCCATAGC CTCCAGCACC ACGTCCATTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCCG TCTTTGCTCA GCCTCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CCCCTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACCGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GTACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTCTTTGGGC AGTCGGCGAG CAGTGCTGCA AGTGTCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACTC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
CCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTCTTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGCA 
ACCTCTGGGT TCAGCTTTTG CCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAATCATC CTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC AGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGGTC AAATAACTCG TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NUP214
Entrez Gene ID	8021
Full Name	nucleoporin 214
Synonyms	CAIN,CAN
General protein information	Preferred Names nucleoporin 214 Names nuclear pore complex protein Nup214 CAN protein, putative oncogene nucleoporin 214kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.13
Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015].
Disorder MIM:	114350
Disorder Html:	Leukemia, acute myeloid, somatic, 601626 (3); Leukemia, T-cell acute lymphoblastic, somatic, 613065 (3)

Pan troglodytes (chimpanzee)	NUP214	XP_001167010.3
	NUP214	XP_001118571.2
Gallus gallus (chicken)	NUP214	XP_425333.4
Homo sapiens (human)	NUP214	NP_005076.3
Mus musculus (house mouse)	Nup214	NP_758472.2
Rattus norvegicus (Norway rat)	Nup214	NP_001162031.1
Canis lupus familiaris (dog)	NUP214	XP_850680.3
Bos taurus (cattle)	NUP214	NP_001192372.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NUP214 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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