SPG11 cDNA ORF clone, Homo sapiens(human)

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu85698 XM_017022636.1
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu85697 XM_017022635.2
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu48597 XM_006720701.3
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu85696 XM_017022634.1
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu16493 NM_001160227.2
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu19157 NM_025137.4
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu48595 XM_006720700.1
Latest version!
Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OHu85697 XM_017022635.1 Homo sapiens spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu16493 NM_001160227.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu19157 NM_025137.3 Homo sapiens SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu85698
    Clone ID Related Accession (Same CDS sequence) XM_017022636.1
    Accession Version XM_017022636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTACCT TGACTGTTAC AAATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAATGCT 
    CAGATTTTGA TTCCCACCAA TCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACC
    CTCCTGGCCC TTGCTACTAC AATGTATTCT CCTGGGGGTG TCAGTCAGGT TGTTCAGAAT
    GAAGAAAATG AAAACTGTTT GAAGAAAGTG GATCCCCAGC TATTGAAGAT GGCATTAACT
    CCTTACCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTGCA CTCCTCCTAG TGTCCTGCCA
    TCTGATATTA CAATCTACCA CCTTATTCAG TCATTATCAC CCTTTGATCC TAGCAGATTG
    TTTGGCTGGC AGTCTGCTAA CACACTAGCT ATAGGAGATG CATGGAGTCA TCTCCCACAT
    TTCTCTAGCC CTGACCTGGT TAATAAATAT GCTATAGTGG AACGTCTGAA TTTTGCTTAT
    TATTTACATA ATGGGCGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAATC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTATAGGG
    CTCTCCTCCT TCCACATACC TTCAATAGGA GCTGCATGTG TTTGTTTCTT AGAATTGCTT
    GGCCTTGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC
    AAGTGCAGAA ATGAAGATGC TCAGTACAGC TTTATCAGAG AGTCTGTAGC CGAAAAACTA
    TCTAAACTAG CTGATGGTGA AAAGACAACC ACAGAAGAAT TGCTTGTTCT CTTAGAAGAA
    GGTACATGGA ACAGCATTCA GCAACAGGAA ATAAAGAGGT TATCCAGTGA ATCTAGCAGC
    CAATGGGCAT TAGTGGTGCA GTTCTGCAGG CTACACAATA TGAAACTAAG CATATCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGATTGG CTGCAGTTCA TTATTCACAG CCAACTCCAC
    AACTACCACC CAGCAGAGGT GAAATCCCTT ATCCAGTACT TCAGCCCAGT CATTCAAGAC
    CACTTAAGGC TGGCTTTTGA GAACTTGCCC TCAGTGCCCA CCTCCAAAAT GGACAGCGAT
    CAAGTCTGCA ATAAGTGCCC CCAGGAACTT CAAGGAAGCA AACAAGAGAT GACCGATTTA
    TTTGAAATTC TGCTCCAATG CTCAGAGGAG CCAGACTCCT GGCACTGGCT TCTGGTTGAA
    GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTTCTGGCCT CATGTCTCCA GGGTGCCAGT
    GCCATTTCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAATGT TGCAACTGAA
    GCAATGGGAC ACATTCAGGA CTCAACAGAG GACCATACCT GGAACCTTGA GGATCTTTCA
    GTCATCTGGA GAACATTATT AACAAGACAA AAGAGCAAAA CTCTCATCAG AGGTTTCCAG
    CTTTTCTTTA AGGATTCCCC GTTACTACTG GTGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAGGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TTCAGAAGAG CCTTGAAACG
    CTTAACACAG CAGCCACAAA GGTCCACCCT GTCATCCCTG CCATGTGGCT GGAGGATCAG
    GTGTGTTTCC TTTTGAAGCT TATGCTACAG CAGTGTAAGA CCCAGTATGA GCTGGGGAAG
    CTTTTACAGC TCTTTGTTGA AAGAGAGCAT CTCTTCTCTG ATGGTCCAGA TGTGAAAAAG
    CTTTGCATCC TTTGCCAGAT TTTGAAGGAT ACATCCATAG CCATTAATCA TACAATTATT
    ACCAGCTACA GCATTGAGAA TCTTCAGCAT GAATGTAGAT CTATTTTGGA AAGACTGCAG
    ACAGATGGAC AATTCGCTTT GGCCAGGAGG GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC
    AACTTGGTTA TTAAAGAGAT AACACAGGAA ATGCAGACCC TAAAACACAT TGAACAGTGG
    TCACTAAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT
    TCAATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAACCC AGGCCCATGT GGCATGTGAG
    CACCCAACTG GATGGAGCAG CATGGAGGAG CGCCATCTGC TGCTCACCTT GGCAGGGCAC
    TGGCTTGCCC AGGAGGACGT GGTGCCCTTG GATAAGCTGG AGGAGCTGGA GAAGCAGATC
    TGGCTGTGCC GCATCACCCA GCACACTCTT GGAAGAAATC AGGAGGAAAC AGAGCCCAGA
    TTTTCTCGAC AGATCTCAAC TAGTGGTGAA CTTTCCTTTG ATAGTTTAGC CAGTGAGTTT
    TCCTTCTCCA AGTTGGCTGC TCTGAACACA TCAAAATACT TAGAACTTAA CAGCCTTCCA
    TCCAAAGAGA CATGCGAGAA TAGATTGGAT TGGAAAGAGC AGGAGTCACT AAACTTTTTG
    ATTGGGCGCC TACTGGATGA TGGCTGTGTG CATGAAGCAA GTAGAGTATG CCGGTATTTT
    CATTTTTATA ATCCAGATGT CGCCTTGGTA TTGCACTGCA GAGCACTGGC CTCAGGGGAA
    GCTAGTATGG AGGATCTGCA CCCAGAGATC CATGCTCTCC TACAAAGTGC TGAGCTGCTT
    GAGGAAGAAG CACCCGACAT TCCCCTAAGG AGAGTCCACA GCACTTCAAG TCTGGATAGT
    CAGAAGTTTG TGACAGTGCC CTCCAGTAAT GAAGTGGTAA CTAACCTGGA AGTGCTGACA
    AGCAAATGCC TCCATGGGAA GAACTACTGT CGACAGGTCC TCTGTCTGTA TGATCTTGCC
    AAGGAGTTGG GCTGTTCCTA CACAGATGTT GCTGCTCAGG ATGGTGAAGC CATGCTCCGG
    AAAATCTTGG CCTCTCAGCA GCCTGACCGA TGCAAACGAG CCCAGGCCTT CATCAGCACA
    CAGGGCCTTA AGCCAGATAC TGTGGCTGAA CTCGTGGCAG AAGAGGTGAC ACGGGAGCTG
    CTTACTTCAT CACAGGGAAC AGGACATAAG CAGATGTTCA ACCCAACAGA GGAAAGCCAG
    ACATTTCTTC AGCTGACCAC TCTGTGTCAA GACCGCACAT TGGTAGGCAT GAAGTTGTTG
    GATAAGATTT CCTCCGTTCC CCATGGGGAA CTGTCTTGCA CCACAGAGCT CCTGATCCTG
    GCCCATCATT GCTTCACCCT GACGTGCCAC ATGGAGGGCA TCATCCGAGT CCTACAGGCC
    GCCCACATGC TCACAGATAA CCACCTGGCC CCCAGTGAGG AGTATGGGCT GGTGGTACGG
    CTCCTCACTG GCATTGGAAG GTACAACGAG ATGACATACA TATTTGATTT GCTGCATAAA
    AAGCACTACT TTGAAGTGCT AATGAGGAAG AAGTTGGATC CGAGTGGTAC CCTGAAAACA
    GCCCTGCTGG ACTACATCAA ACGCTGCCGT CCTGGAGACA GTGAAAAGCA CAATATGATT
    GCCCTGTGCT TCAGCATGTG CCGGGAGATT GGCGAGAACC ACGAGGCAGC TGCCCGCATC
    CAACTGAAAT TGATTGAGTC TCAGCCCTGG GAGGACAGCC TCAAGGATGG GCACCAGCTG
    AAACAACTGC TGCTGAAGGC CCTGACTCTG ATGTTGGATG CAGCAGAGAG TTATGCCAAG
    GACTCCTGTG TGCGACAGGC CCAGCACTGT CAGCGGCTCA CCAAGTTGAT AACTCTGCAG
    ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACTTGGGCCG CCACAAGCTG
    ATGGACTGTA TTCTGGCCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC TGAGGCCTAC
    GATTTTGTTC CAGATTGGGC TGAAATTTTA TACCAGCAAG TGATTCTTAA AGGAGACTTT
    AATTACTTGG AAGAATTTAA GCAGCAAAGG TTATTAAAGT CCAGTATATT TGAAGAGATT
    TCCAAAAAAT ATAAACAACA TCAGCCTACT GACATGGTCA TGGAAAACCT GAAGAAATTA
    CTCACATATT GTGAAGATGT TTACCTGTAT TACAAGTTGG CATACGAACA CAAGTTTTAT
    GAAATTGTAA ATGTGCTTCT GAAGGACCCT CAGACAGGTT GCTGTCTAAA GGACATGCTA
    GCAGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878125.1
    CDS964..5172
    Translation

    Target ORF information:

    RefSeq Version XM_017022636.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTACCT TGACTGTTAC AAATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAATGCT 
    CAGATTTTGA TTCCCACCAA TCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACC
    CTCCTGGCCC TTGCTACTAC AATGTATTCT CCTGGGGGTG TCAGTCAGGT TGTTCAGAAT
    GAAGAAAATG AAAACTGTTT GAAGAAAGTG GATCCCCAGC TATTGAAGAT GGCATTAACT
    CCTTACCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTGCA CTCCTCCTAG TGTCCTGCCA
    TCTGATATTA CAATCTACCA CCTTATTCAG TCATTATCAC CCTTTGATCC TAGCAGATTG
    TTTGGCTGGC AGTCTGCTAA CACACTAGCT ATAGGAGATG CATGGAGTCA TCTCCCACAT
    TTCTCTAGCC CTGACCTGGT TAATAAATAT GCTATAGTGG AACGTCTGAA TTTTGCTTAT
    TATTTACATA ATGGGCGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAATC
    AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTATAGGG
    CTCTCCTCCT TCCACATACC TTCAATAGGA GCTGCATGTG TTTGTTTCTT AGAATTGCTT
    GGCCTTGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC
    AAGTGCAGAA ATGAAGATGC TCAGTACAGC TTTATCAGAG AGTCTGTAGC CGAAAAACTA
    TCTAAACTAG CTGATGGTGA AAAGACAACC ACAGAAGAAT TGCTTGTTCT CTTAGAAGAA
    GGTACATGGA ACAGCATTCA GCAACAGGAA ATAAAGAGGT TATCCAGTGA ATCTAGCAGC
    CAATGGGCAT TAGTGGTGCA GTTCTGCAGG CTACACAATA TGAAACTAAG CATATCTTAC
    CTTAGAGAAT GTGCCAAAGC AAATGATTGG CTGCAGTTCA TTATTCACAG CCAACTCCAC
    AACTACCACC CAGCAGAGGT GAAATCCCTT ATCCAGTACT TCAGCCCAGT CATTCAAGAC
    CACTTAAGGC TGGCTTTTGA GAACTTGCCC TCAGTGCCCA CCTCCAAAAT GGACAGCGAT
    CAAGTCTGCA ATAAGTGCCC CCAGGAACTT CAAGGAAGCA AACAAGAGAT GACCGATTTA
    TTTGAAATTC TGCTCCAATG CTCAGAGGAG CCAGACTCCT GGCACTGGCT TCTGGTTGAA
    GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTTCTGGCCT CATGTCTCCA GGGTGCCAGT
    GCCATTTCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAATGT TGCAACTGAA
    GCAATGGGAC ACATTCAGGA CTCAACAGAG GACCATACCT GGAACCTTGA GGATCTTTCA
    GTCATCTGGA GAACATTATT AACAAGACAA AAGAGCAAAA CTCTCATCAG AGGTTTCCAG
    CTTTTCTTTA AGGATTCCCC GTTACTACTG GTGATGGAGA TGTATGAACT GTGTATGTTC
    TTCAGGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TTCAGAAGAG CCTTGAAACG
    CTTAACACAG CAGCCACAAA GGTCCACCCT GTCATCCCTG CCATGTGGCT GGAGGATCAG
    GTGTGTTTCC TTTTGAAGCT TATGCTACAG CAGTGTAAGA CCCAGTATGA GCTGGGGAAG
    CTTTTACAGC TCTTTGTTGA AAGAGAGCAT CTCTTCTCTG ATGGTCCAGA TGTGAAAAAG
    CTTTGCATCC TTTGCCAGAT TTTGAAGGAT ACATCCATAG CCATTAATCA TACAATTATT
    ACCAGCTACA GCATTGAGAA TCTTCAGCAT GAATGTAGAT CTATTTTGGA AAGACTGCAG
    ACAGATGGAC AATTCGCTTT GGCCAGGAGG GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC
    AACTTGGTTA TTAAAGAGAT AACACAGGAA ATGCAGACCC TAAAACACAT TGAACAGTGG
    TCACTAAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT
    TCAATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAACCC AGGCCCATGT GGCATGTGAG
    CACCCAACTG GATGGAGCAG CATGGAGGAG CGCCATCTGC TGCTCACCTT GGCAGGGCAC
    TGGCTTGCCC AGGAGGACGT GGTGCCCTTG GATAAGCTGG AGGAGCTGGA GAAGCAGATC
    TGGCTGTGCC GCATCACCCA GCACACTCTT GGAAGAAATC AGGAGGAAAC AGAGCCCAGA
    TTTTCTCGAC AGATCTCAAC TAGTGGTGAA CTTTCCTTTG ATAGTTTAGC CAGTGAGTTT
    TCCTTCTCCA AGTTGGCTGC TCTGAACACA TCAAAATACT TAGAACTTAA CAGCCTTCCA
    TCCAAAGAGA CATGCGAGAA TAGATTGGAT TGGAAAGAGC AGGAGTCACT AAACTTTTTG
    ATTGGGCGCC TACTGGATGA TGGCTGTGTG CATGAAGCAA GTAGAGTATG CCGGTATTTT
    CATTTTTATA ATCCAGATGT CGCCTTGGTA TTGCACTGCA GAGCACTGGC CTCAGGGGAA
    GCTAGTATGG AGGATCTGCA CCCAGAGATC CATGCTCTCC TACAAAGTGC TGAGCTGCTT
    GAGGAAGAAG CACCCGACAT TCCCCTAAGG AGAGTCCACA GCACTTCAAG TCTGGATAGT
    CAGAAGTTTG TGACAGTGCC CTCCAGTAAT GAAGTGGTAA CTAACCTGGA AGTGCTGACA
    AGCAAATGCC TCCATGGGAA GAACTACTGT CGACAGGTCC TCTGTCTGTA TGATCTTGCC
    AAGGAGTTGG GCTGTTCCTA CACAGATGTT GCTGCTCAGG ATGGTGAAGC CATGCTCCGG
    AAAATCTTGG CCTCTCAGCA GCCTGACCGA TGCAAACGAG CCCAGGCCTT CATCAGCACA
    CAGGGCCTTA AGCCAGATAC TGTGGCTGAA CTCGTGGCAG AAGAGGTGAC ACGGGAGCTG
    CTTACTTCAT CACAGGGAAC AGGACATAAG CAGATGTTCA ACCCAACAGA GGAAAGCCAG
    ACATTTCTTC AGCTGACCAC TCTGTGTCAA GACCGCACAT TGGTAGGCAT GAAGTTGTTG
    GATAAGATTT CCTCCGTTCC CCATGGGGAA CTGTCTTGCA CCACAGAGCT CCTGATCCTG
    GCCCATCATT GCTTCACCCT GACGTGCCAC ATGGAGGGCA TCATCCGAGT CCTACAGGCC
    GCCCACATGC TCACAGATAA CCACCTGGCC CCCAGTGAGG AGTATGGGCT GGTGGTACGG
    CTCCTCACTG GCATTGGAAG GTACAACGAG ATGACATACA TATTTGATTT GCTGCATAAA
    AAGCACTACT TTGAAGTGCT AATGAGGAAG AAGTTGGATC CGAGTGGTAC CCTGAAAACA
    GCCCTGCTGG ACTACATCAA ACGCTGCCGT CCTGGAGACA GTGAAAAGCA CAATATGATT
    GCCCTGTGCT TCAGCATGTG CCGGGAGATT GGCGAGAACC ACGAGGCAGC TGCCCGCATC
    CAACTGAAAT TGATTGAGTC TCAGCCCTGG GAGGACAGCC TCAAGGATGG GCACCAGCTG
    AAACAACTGC TGCTGAAGGC CCTGACTCTG ATGTTGGATG CAGCAGAGAG TTATGCCAAG
    GACTCCTGTG TGCGACAGGC CCAGCACTGT CAGCGGCTCA CCAAGTTGAT AACTCTGCAG
    ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACTTGGGCCG CCACAAGCTG
    ATGGACTGTA TTCTGGCCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC TGAGGCCTAC
    GATTTTGTTC CAGATTGGGC TGAAATTTTA TACCAGCAAG TGATTCTTAA AGGAGACTTT
    AATTACTTGG AAGAATTTAA GCAGCAAAGG TTATTAAAGT CCAGTATATT TGAAGAGATT
    TCCAAAAAAT ATAAACAACA TCAGCCTACT GACATGGTCA TGGAAAACCT GAAGAAATTA
    CTCACATATT GTGAAGATGT TTACCTGTAT TACAAGTTGG CATACGAACA CAAGTTTTAT
    GAAATTGTAA ATGTGCTTCT GAAGGACCCT CAGACAGGTT GCTGTCTAAA GGACATGCTA
    GCAGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85697
    Clone ID Related Accession (Same CDS sequence) XM_017022635.1 , XM_017022635.2
    Accession Version XM_017022635.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878124.1
    CDS32..4834
    Translation

    Target ORF information:

    RefSeq Version XM_017022635.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85697
    Clone ID Related Accession (Same CDS sequence) XM_017022635.1 , XM_017022635.2
    Accession Version XM_017022635.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878124.1
    CDS32..4834
    Translation

    Target ORF information:

    RefSeq Version XM_017022635.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022635.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48597
    Clone ID Related Accession (Same CDS sequence) XM_006720701.3
    Accession Version XM_006720701.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGGTCCAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720764.1
    CDS32..4786
    Translation

    Target ORF information:

    RefSeq Version XM_006720701.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720701.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGGTCCAGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85696
    Clone ID Related Accession (Same CDS sequence) XM_017022634.1
    Accession Version XM_017022634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7224bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGAGT
    GGTACCCTGA AAACAGCCCT GCTGGACTAC ATCAAACGCT GCCGTCCTGG AGACAGTGAA
    AAGCACAATA TGATTGCCCT GTGCTTCAGC ATGTGCCGGG AGATTGGCGA GAACCACGAG
    GCAGCTGCCC GCATCCAACT GAAATTGATT GAGTCTCAGC CCTGGGAGGA CAGCCTCAAG
    GATGGGCACC AGCTGAAACA ACTGCTGCTG AAGGCCCTGA CTCTGATGTT GGATGCAGCA
    GAGAGTTATG CCAAGGACTC CTGTGTGCGA CAGGCCCAGC ACTGTCAGCG GCTCACCAAG
    TTGATAACTC TGCAGATTCA CTTTCTGAAC ACTGGCCAGA ACACAATGCT CATCAACTTG
    GGCCGCCACA AGCTGATGGA CTGTATTCTG GCCCTACCTC GGTTCTACCA GGCTTCTATT
    GTGGCTGAGG CCTACGATTT TGTTCCAGAT TGGGCTGAAA TTTTATACCA GCAAGTGATT
    CTTAAAGGAG ACTTTAATTA CTTGGAAGAA TTTAAGCAGC AAAGGTTATT AAAGTCCAGT
    ATATTTGAAG AGATTTCCAA AAAATATAAA CAACATCAGC CTACTGACAT GGTCATGGAA
    AACCTGAAGA AATTACTCAC ATATTGTGAA GATGTTTACC TGTATTACAA GTTGGCATAC
    GAACACAAGT TTTATGAAAT TGTAAATGTG CTTCTGAAGG ACCCTCAGAC AGGTTGCTGT
    CTAAAGGACA TGCTAGCAGG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878123.1
    CDS32..7255
    Translation

    Target ORF information:

    RefSeq Version XM_017022634.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGAGT
    GGTACCCTGA AAACAGCCCT GCTGGACTAC ATCAAACGCT GCCGTCCTGG AGACAGTGAA
    AAGCACAATA TGATTGCCCT GTGCTTCAGC ATGTGCCGGG AGATTGGCGA GAACCACGAG
    GCAGCTGCCC GCATCCAACT GAAATTGATT GAGTCTCAGC CCTGGGAGGA CAGCCTCAAG
    GATGGGCACC AGCTGAAACA ACTGCTGCTG AAGGCCCTGA CTCTGATGTT GGATGCAGCA
    GAGAGTTATG CCAAGGACTC CTGTGTGCGA CAGGCCCAGC ACTGTCAGCG GCTCACCAAG
    TTGATAACTC TGCAGATTCA CTTTCTGAAC ACTGGCCAGA ACACAATGCT CATCAACTTG
    GGCCGCCACA AGCTGATGGA CTGTATTCTG GCCCTACCTC GGTTCTACCA GGCTTCTATT
    GTGGCTGAGG CCTACGATTT TGTTCCAGAT TGGGCTGAAA TTTTATACCA GCAAGTGATT
    CTTAAAGGAG ACTTTAATTA CTTGGAAGAA TTTAAGCAGC AAAGGTTATT AAAGTCCAGT
    ATATTTGAAG AGATTTCCAA AAAATATAAA CAACATCAGC CTACTGACAT GGTCATGGAA
    AACCTGAAGA AATTACTCAC ATATTGTGAA GATGTTTACC TGTATTACAA GTTGGCATAC
    GAACACAAGT TTTATGAAAT TGTAAATGTG CTTCTGAAGG ACCCTCAGAC AGGTTGCTGT
    CTAAAGGACA TGCTAGCAGG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16493
    Clone ID Related Accession (Same CDS sequence) NM_001160227.1 , NM_001160227.2
    Accession Version NM_001160227.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product spatacsin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA926289.1, BC150640.1 and BC094704.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG
    TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC
    ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT
    TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG
    GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT
    GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA
    TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG
    GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA
    GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG
    AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC
    AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG
    GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG
    CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC
    ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG
    GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG
    CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA
    AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG
    GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG
    TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA
    GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153699.1
    CDS32..7024
    Translation

    Target ORF information:

    RefSeq Version NM_001160227.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG
    TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC
    ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT
    TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG
    GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT
    GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA
    TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG
    GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA
    GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG
    AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC
    AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG
    GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG
    CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC
    ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG
    GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG
    CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA
    AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG
    GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG
    TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA
    GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16493
    Clone ID Related Accession (Same CDS sequence) NM_001160227.1 , NM_001160227.2
    Accession Version NM_001160227.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product spatacsin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC150640.1 and BC094704.1. On May 31, 2019 this sequence version replaced NM_001160227.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG
    TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC
    ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT
    TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG
    GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT
    GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA
    TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG
    GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA
    GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG
    AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC
    AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG
    GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG
    CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC
    ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG
    GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG
    CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA
    AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG
    GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG
    TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA
    GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153699.1
    CDS16..7008
    Translation

    Target ORF information:

    RefSeq Version NM_001160227.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG
    TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC
    ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT
    TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG
    GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT
    GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA
    TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG
    GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA
    GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG
    AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC
    AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG
    GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG
    CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC
    ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG
    GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG
    CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA
    AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG
    GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG
    TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA
    GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19157
    Clone ID Related Accession (Same CDS sequence) NM_025137.4 , NM_025137.3
    Accession Version NM_025137.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product spatacsin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009996.7, AB058743.2 and BC094704.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_025137.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153879.1, AB058743.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261866.12/ ENSP00000261866.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA
    CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT
    AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA
    ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG
    ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC
    ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG
    CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC
    AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG
    CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG
    CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC
    TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC
    TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG
    ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA
    TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT
    TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG
    CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079413.3
    CDS16..7347
    Translation

    Target ORF information:

    RefSeq Version NM_025137.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025137.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA
    CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT
    AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA
    ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG
    ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC
    ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG
    CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC
    AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG
    CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG
    CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC
    TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC
    TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG
    ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA
    TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT
    TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG
    CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19157
    Clone ID Related Accession (Same CDS sequence) NM_025137.4 , NM_025137.3
    Accession Version NM_025137.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product spatacsin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009996.7, AB058743.2 and BC094704.1. This sequence is a reference standard in the RefSeqGene project. On Apr 22, 2006 this sequence version replaced NM_025137.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153879.1, AB058743.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA
    CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT
    AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA
    ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG
    ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC
    ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG
    CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC
    AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG
    CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG
    CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC
    TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC
    TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG
    ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA
    TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT
    TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG
    CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079413.3
    CDS32..7363
    Translation

    Target ORF information:

    RefSeq Version NM_025137.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025137.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG
    TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA
    AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT
    GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG
    CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG
    ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC
    AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG
    TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT
    CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT
    TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT
    TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG
    GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG
    CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT
    AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG
    ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT
    GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC
    CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC
    ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG
    CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC
    CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG
    TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC
    CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG
    GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA
    CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT
    AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA
    ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG
    ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC
    ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG
    CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC
    AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG
    CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG
    CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC
    TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC
    TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG
    ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA
    TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT
    TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG
    CTAGCAGGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48595
    Clone ID Related Accession (Same CDS sequence) XM_006720700.1
    Accession Version XM_006720700.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GGCCCATGTG GCATGTGAGC ACCCAACTGG ATGGAGCAGC
    ATGGAGGAGC GCCATCTGCT GCTCACCTTG GCAGGGCACT GGCTTGCCCA GGAGGACGTG
    GTGCCCTTGG ATAAGCTGGA GGAGCTGGAG AAGCAGATCT GGCTGTGCCG CATCACCCAG
    CACACTCTTG GAAGAAATCA GGAGGAAACA GAGCCCAGAT TTTCTCGACA GATCTCAACT
    AGTGGTGAAC TTTCCTTTGA TAGTTTAGCC AGTGAGTTTT CCTTCTCCAA GTTGGCTGCT
    CTGAACACAT CAAAATACTT AGAACTTAAC AGCCTTCCAT CCAAAGAGAC ATGCGAGAAT
    AGATTGGATT GGAAAGAGCA GGAGTCACTA AACTTTTTGA TTGGGCGCCT ACTGGATGAT
    GGCTGTGTGC ATGAAGCAAG TAGAGTATGC CGGTATTTTC ATTTTTATAA TCCAGATGTC
    GCCTTGGTAT TGCACTGCAG AGCACTGGCC TCAGGGGAAG CTAGTATGGA GGATCTGCAC
    CCAGAGATCC ATGCTCTCCT ACAAAGTGCT GAGCTGCTTG AGGAAGAAGC ACCCGACATT
    CCCCTAAGGA GAGTCCACAG CACTTCAAGT CTGGATAGTC AGAAGTTTGT GACAGTGCCC
    TCCAGTAATG AAGTGGTAAC TAACCTGGAA GTGCTGACAA GCAAATGCCT CCATGGGAAG
    AACTACTGTC GACAGGTCCT CTGTCTGTAT GATCTTGCCA AGGAGTTGGG CTGTTCCTAC
    ACAGATGTTG CTGCTCAGGA TGGTGAAGCC ATGCTCCGGA AAATCTTGGC CTCTCAGCAG
    CCTGACCGAT GCAAACGAGC CCAGGCCTTC ATCAGCACAC AGGGCCTTAA GCCAGATACT
    GTGGCTGAAC TCGTGGCAGA AGAGGTGACA CGGGAGCTGC TTACTTCATC ACAGGGAACA
    GGACATAAGC AGATGTTCAA CCCAACAGAG GAAAGCCAGA CATTTCTTCA GCTGACCACT
    CTGTGTCAAG ACCGCACATT GGTAGGCATG AAGTTGTTGG ATAAGATTTC CTCCGTTCCC
    CATGGGGAAC TGTCTTGCAC CACAGAGCTC CTGATCCTGG CCCATCATTG CTTCACCCTG
    ACGTGCCACA TGGAGGGCAT CATCCGAGTC CTACAGGCCG CCCACATGCT CACAGATAAC
    CACCTGGCCC CCAGTGAGGA GTATGGGCTG GTGGTACGGC TCCTCACTGG CATTGGAAGG
    TACAACGAGA TGACATACAT ATTTGATTTG CTGCATAAAA AGCACTACTT TGAAGTGCTA
    ATGAGGAAGA AGTTGGATCC GAGTGGTACC CTGAAAACAG CCCTGCTGGA CTACATCAAA
    CGCTGCCGTC CTGGAGACAG TGAAAAGCAC AATATGATTG CCCTGTGCTT CAGCATGTGC
    CGGGAGATTG GCGAGAACCA CGAGGCAGCT GCCCGCATCC AACTGAAATT GATTGAGTCT
    CAGCCCTGGG AGGACAGCCT CAAGGATGGG CACCAGCTGA AACAACTGCT GCTGAAGGCC
    CTGACTCTGA TGTTGGATGC AGCAGAGAGT TATGCCAAGG ACTCCTGTGT GCGACAGGCC
    CAGCACTGTC AGCGGCTCAC CAAGTTGATA ACTCTGCAGA TTCACTTTCT GAACACTGGC
    CAGAACACAA TGCTCATCAA CTTGGGCCGC CACAAGCTGA TGGACTGTAT TCTGGCCCTA
    CCTCGGTTCT ACCAGGCTTC TATTGTGGCT GAGGCCTACG ATTTTGTTCC AGATTGGGCT
    GAAATTTTAT ACCAGCAAGT GATTCTTAAA GGAGACTTTA ATTACTTGGA AGAATTTAAG
    CAGCAAAGGT TATTAAAGTC CAGTATATTT GAAGAGATTT CCAAAAAATA TAAACAACAT
    CAGCCTACTG ACATGGTCAT GGAAAACCTG AAGAAATTAC TCACATATTG TGAAGATGTT
    TACCTGTATT ACAAGTTGGC ATACGAACAC AAGTTTTATG AAATTGTAAA TGTGCTTCTG
    AAGGACCCTC AGACAGGTTG CTGTCTAAAG GACATGCTAG CAGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720763.1
    CDS32..7219
    Translation

    Target ORF information:

    RefSeq Version XM_006720700.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720700.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
    GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG
    CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG
    GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG
    GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA
    AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG
    AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG
    CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC
    AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA
    AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG
    GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC
    TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT
    CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT
    TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA
    GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA
    ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC
    TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC
    CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT
    CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT
    GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG
    AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA
    CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT
    TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG
    CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG
    TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG
    TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC
    AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT
    CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA
    AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG
    GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA
    CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC
    CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG
    AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG
    CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC
    AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA
    ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG
    CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA
    GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC
    TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT
    TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT
    TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA
    CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT
    GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA
    ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT
    GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT
    GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG
    ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA
    GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC
    CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC
    AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT
    CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC
    TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT
    CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT
    GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT
    ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG
    AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA
    ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG
    CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA
    TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA
    CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT
    TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA
    ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA
    GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG
    CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC
    TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA
    CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA
    GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC
    AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT
    TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC
    CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA
    GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC
    GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT
    TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT
    GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC
    AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT
    GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT
    TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC
    CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG
    TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA
    ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT
    CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG
    AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA
    AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT
    ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG
    CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG
    GACAACTTGG TTATTAAAGA GGCCCATGTG GCATGTGAGC ACCCAACTGG ATGGAGCAGC
    ATGGAGGAGC GCCATCTGCT GCTCACCTTG GCAGGGCACT GGCTTGCCCA GGAGGACGTG
    GTGCCCTTGG ATAAGCTGGA GGAGCTGGAG AAGCAGATCT GGCTGTGCCG CATCACCCAG
    CACACTCTTG GAAGAAATCA GGAGGAAACA GAGCCCAGAT TTTCTCGACA GATCTCAACT
    AGTGGTGAAC TTTCCTTTGA TAGTTTAGCC AGTGAGTTTT CCTTCTCCAA GTTGGCTGCT
    CTGAACACAT CAAAATACTT AGAACTTAAC AGCCTTCCAT CCAAAGAGAC ATGCGAGAAT
    AGATTGGATT GGAAAGAGCA GGAGTCACTA AACTTTTTGA TTGGGCGCCT ACTGGATGAT
    GGCTGTGTGC ATGAAGCAAG TAGAGTATGC CGGTATTTTC ATTTTTATAA TCCAGATGTC
    GCCTTGGTAT TGCACTGCAG AGCACTGGCC TCAGGGGAAG CTAGTATGGA GGATCTGCAC
    CCAGAGATCC ATGCTCTCCT ACAAAGTGCT GAGCTGCTTG AGGAAGAAGC ACCCGACATT
    CCCCTAAGGA GAGTCCACAG CACTTCAAGT CTGGATAGTC AGAAGTTTGT GACAGTGCCC
    TCCAGTAATG AAGTGGTAAC TAACCTGGAA GTGCTGACAA GCAAATGCCT CCATGGGAAG
    AACTACTGTC GACAGGTCCT CTGTCTGTAT GATCTTGCCA AGGAGTTGGG CTGTTCCTAC
    ACAGATGTTG CTGCTCAGGA TGGTGAAGCC ATGCTCCGGA AAATCTTGGC CTCTCAGCAG
    CCTGACCGAT GCAAACGAGC CCAGGCCTTC ATCAGCACAC AGGGCCTTAA GCCAGATACT
    GTGGCTGAAC TCGTGGCAGA AGAGGTGACA CGGGAGCTGC TTACTTCATC ACAGGGAACA
    GGACATAAGC AGATGTTCAA CCCAACAGAG GAAAGCCAGA CATTTCTTCA GCTGACCACT
    CTGTGTCAAG ACCGCACATT GGTAGGCATG AAGTTGTTGG ATAAGATTTC CTCCGTTCCC
    CATGGGGAAC TGTCTTGCAC CACAGAGCTC CTGATCCTGG CCCATCATTG CTTCACCCTG
    ACGTGCCACA TGGAGGGCAT CATCCGAGTC CTACAGGCCG CCCACATGCT CACAGATAAC
    CACCTGGCCC CCAGTGAGGA GTATGGGCTG GTGGTACGGC TCCTCACTGG CATTGGAAGG
    TACAACGAGA TGACATACAT ATTTGATTTG CTGCATAAAA AGCACTACTT TGAAGTGCTA
    ATGAGGAAGA AGTTGGATCC GAGTGGTACC CTGAAAACAG CCCTGCTGGA CTACATCAAA
    CGCTGCCGTC CTGGAGACAG TGAAAAGCAC AATATGATTG CCCTGTGCTT CAGCATGTGC
    CGGGAGATTG GCGAGAACCA CGAGGCAGCT GCCCGCATCC AACTGAAATT GATTGAGTCT
    CAGCCCTGGG AGGACAGCCT CAAGGATGGG CACCAGCTGA AACAACTGCT GCTGAAGGCC
    CTGACTCTGA TGTTGGATGC AGCAGAGAGT TATGCCAAGG ACTCCTGTGT GCGACAGGCC
    CAGCACTGTC AGCGGCTCAC CAAGTTGATA ACTCTGCAGA TTCACTTTCT GAACACTGGC
    CAGAACACAA TGCTCATCAA CTTGGGCCGC CACAAGCTGA TGGACTGTAT TCTGGCCCTA
    CCTCGGTTCT ACCAGGCTTC TATTGTGGCT GAGGCCTACG ATTTTGTTCC AGATTGGGCT
    GAAATTTTAT ACCAGCAAGT GATTCTTAAA GGAGACTTTA ATTACTTGGA AGAATTTAAG
    CAGCAAAGGT TATTAAAGTC CAGTATATTT GAAGAGATTT CCAAAAAATA TAAACAACAT
    CAGCCTACTG ACATGGTCAT GGAAAACCTG AAGAAATTAC TCACATATTG TGAAGATGTT
    TACCTGTATT ACAAGTTGGC ATACGAACAC AAGTTTTATG AAATTGTAAA TGTGCTTCTG
    AAGGACCCTC AGACAGGTTG CTGTCTAAAG GACATGCTAG CAGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.