SPSB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPSB1
Entrez Gene ID	80176
Full Name	splA/ryanodine receptor domain and SOCS box containing 1
Synonyms	SSB-1,SSB1
General protein information	Preferred Names splA/ryanodine receptor domain and SOCS box containing 1 Names SPRY domain-containing SOCS box protein 1 SPRY domain-containing SOCS box protein SSB-1 novel SPRY domain containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.22
Disorder MIM:	611657

mRNA and Protein(s)

mRNA	Protein	Name
NM_025106.3	NP_079382.2	SPRY domain-containing SOCS box protein 1
NM_025106.4	NP_079382.2	SPRY domain-containing SOCS box protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPSB1	XP_525174.3
	SPSB1	XP_002802207.1
Canis lupus familiaris (dog)	SPSB1	XP_005620557.1
Mus musculus (house mouse)	Spsb1	NP_083311.1
Rattus norvegicus (Norway rat)	Spsb1	NP_001101464.1
Gallus gallus (chicken)	SPSB1	XP_417599.1
Danio rerio (zebrafish)	spsb1	NP_001020631.1
Xenopus tropicalis (tropical clawed frog)	spsb1	XP_002936603.1
Homo sapiens (human)	SPSB1	NP_079382.2
Bos taurus (cattle)	SPSB1	NP_001029381.1

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.
Okumura F, Joo-Okumura A, Obara K, Petersen A, Nishikimi A, Fukui Y, Nakatsukasa K, Kamura T
Molecular biology of the cell28(24)3532-3541(2017 Nov)

SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling.
Wang F, Fu X, Chen P, Wu P, Fan X, Li N, Zhu H, Jia TT, Ji H, Wang Z, Wong CC, Hu R, Hui J
Cell research27(4)540-558(2017 Apr)

SPSB1, a Novel Negative Regulator of the Transforming Growth Factor-? Signaling Pathway Targeting the Type II Receptor.
Liu S, Nheu T, Luwor R, Nicholson SE, Zhu HJ
The Journal of biological chemistry290(29)17894-908(2015 Jul)

PKD1 phosphorylation-dependent degradation of SNAIL by SCF-FBXO11 regulates epithelial-mesenchymal transition and metastasis.
Zheng H, Shen M, Zha YL, Li W, Wei Y, Blanco MA, Ren G, Zhou T, Storz P, Wang HY, Kang Y
Cancer cell26(3)358-373(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EGF signaling upregulates an E3 ubiquitin (Ub) ligase adaptor, SPRY domain-containing SOCS box protein 1 (SPSB1), which recruits Elongin B/C-Cullin complexes to conjugate lysine 29-linked poly Ubiquitin chains onto hnRNP A1.
Title: SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling.

SPSB1 negatively regulates the TGF-beta signaling pathway through its interaction with both endogenous and overexpressed TbetaRII (and not TbetaRI) via its Spry domain.
Title: SPSB1, a Novel Negative Regulator of the Transforming Growth Factor-? Signaling Pathway Targeting the Type II Receptor.

Findings define a novel pathway that contributes to breast cancer recurrence and provide evidence implicating SPSB proteins in cancer.
Title: SPSB1 promotes breast cancer recurrence by potentiating c-MET signaling.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

SSB1/2 is part of an ssDNA-binding heterotrimeric complex, SOSS which is involved in the maintenance of genome stability.
Title: SOSS complexes participate in the maintenance of genomic stability.

The following SPSB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPSB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24458	NM_025106.4 Latest version!	Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu24458	NM_025106.3	Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPSB1 ( NM_025106.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079382.2 Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.

Price & Availability↑

CloneID	OHu24458
Clone ID Related Accession (Same CDS sequence)	NM_025106.3 , NM_025106.4
Accession Version	NM_025106.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	SPRY domain-containing SOCS box protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB242202.1, AL008734.10, BC015711.1 and AL358252.13. On Oct 31, 2008 this sequence version replaced NM_025106.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026046.1, AK225417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGA AGGTCACTGG AGGGATCAAG ACTGTGGACA TGAGGGACCC CACGTACAGG 
CCCCTGAAGC AGGAGCTCCA GGGTCTGGAT TACTGCAAGC CCACCCGGCT GGATCTGCTA 
CTGGACATGC CCCCTGTGTC CTATGATGTC CAGCTGCTGC ATTCATGGAA CAACAACGAC 
CGATCGCTCA ATGTCTTTGT GAAGGAGGAC GACAAGCTCA TCTTTCACCG GCATCCGGTG 
GCCCAGAGCA CGGACGCTAT CAGGGGCAAA GTCGGGTATA CCCGTGGGCT GCACGTGTGG 
CAGATCACGT GGGCCATGAG ACAGCGGGGC ACACACGCCG TGGTGGGGGT GGCGACGGCA 
GACGCCCCCC TGCACTCTGT CGGGTACACA ACCCTCGTGG GGAATAACCA CGAGTCCTGG 
GGCTGGGACT TGGGGCGCAA CCGGCTCTAC CACGATGGCA AGAACCAGCC AAGCAAAACA 
TACCCAGCCT TTCTGGAACC AGATGAGACA TTCATTGTCC CTGACTCCTT CCTGGTAGCC 
CTGGACATGG ACGACGGGAC TCTGAGCTTC ATTGTGGATG GACAGTACAT GGGAGTGGCT 
TTTCGGGGAC TCAAGGGCAA AAAACTGTAT CCTGTAGTGA GTGCCGTCTG GGGCCACTGT 
GAGATCCGAA TGCGCTACTT GAACGGACTC GATCCCGAGC CGCTGCCGCT CATGGATTTG 
TGCCGTCGCT CGGTGCGCCT GGCCCTGGGG AGGGAGCGCC TGGGGGAGAT CCACACGCTG 
CCGCTGCCGG CTTCCCTCAA GGCCTACCTC CTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079382.2
CDS	340..1161
Translation	MGQKVTGGIKTVDMRDPTYRPLKQELQGLDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFVKEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLGRNRLYHDGKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFRGLKGKKLYPVVSAVWGHCEIRMRYLNGLDPEPLPLMDLCRRSVRLALGRERLGEIHTLPLPASLKAYLLYQ

Target ORF information:

RefSeq Version	NM_025106.3
Organism	Homo sapiens(human)
Definition	Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025106.3

ORF Insert Sequence:

ATGGGTCAGA AGGTCACTGG AGGGATCAAG ACTGTGGACA TGAGGGACCC CACGTACAGG 
CCCCTGAAGC AGGAGCTCCA GGGTCTGGAT TACTGCAAGC CCACCCGGCT GGATCTGCTA 
CTGGACATGC CCCCTGTGTC CTATGATGTC CAGCTGCTGC ATTCATGGAA CAACAACGAC 
CGATCGCTCA ATGTCTTTGT GAAGGAGGAC GACAAGCTCA TCTTTCACCG GCATCCGGTG 
GCCCAGAGCA CGGACGCTAT CAGGGGCAAA GTCGGGTATA CCCGTGGGCT GCACGTGTGG 
CAGATCACGT GGGCCATGAG ACAGCGGGGC ACACACGCCG TGGTGGGGGT GGCGACGGCA 
GACGCCCCCC TGCACTCTGT CGGGTACACA ACCCTCGTGG GGAATAACCA CGAGTCCTGG 
GGCTGGGACT TGGGGCGCAA CCGGCTCTAC CACGATGGCA AGAACCAGCC AAGCAAAACA 
TACCCAGCCT TTCTGGAACC AGATGAGACA TTCATTGTCC CTGACTCCTT CCTGGTAGCC 
CTGGACATGG ACGACGGGAC TCTGAGCTTC ATTGTGGATG GACAGTACAT GGGAGTGGCT 
TTTCGGGGAC TCAAGGGCAA AAAACTGTAT CCTGTAGTGA GTGCCGTCTG GGGCCACTGT 
GAGATCCGAA TGCGCTACTT GAACGGACTC GATCCCGAGC CGCTGCCGCT CATGGATTTG 
TGCCGTCGCT CGGTGCGCCT GGCCCTGGGG AGGGAGCGCC TGGGGGAGAT CCACACGCTG 
CCGCTGCCGG CTTCCCTCAA GGCCTACCTC CTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPSB1 ( NM_025106.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079382.2 Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.

Price & Availability↑

CloneID	OHu24458
Clone ID Related Accession (Same CDS sequence)	NM_025106.3 , NM_025106.4
Accession Version	NM_025106.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	SPRY domain-containing SOCS box protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB242202.1, AL008734.10, BC015711.1 and AL358252.13. On Nov 22, 2018 this sequence version replaced NM_025106.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.488534.1, AK026046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328089.11/ ENSP00000330221.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCAGA AGGTCACTGG AGGGATCAAG ACTGTGGACA TGAGGGACCC CACGTACAGG 
CCCCTGAAGC AGGAGCTCCA GGGTCTGGAT TACTGCAAGC CCACCCGGCT GGATCTGCTA 
CTGGACATGC CCCCTGTGTC CTATGATGTC CAGCTGCTGC ATTCATGGAA CAACAACGAC 
CGATCGCTCA ATGTCTTTGT GAAGGAGGAC GACAAGCTCA TCTTTCACCG GCATCCGGTG 
GCCCAGAGCA CGGACGCTAT CAGGGGCAAA GTCGGGTATA CCCGTGGGCT GCACGTGTGG 
CAGATCACGT GGGCCATGAG ACAGCGGGGC ACACACGCCG TGGTGGGGGT GGCGACGGCA 
GACGCCCCCC TGCACTCTGT CGGGTACACA ACCCTCGTGG GGAATAACCA CGAGTCCTGG 
GGCTGGGACT TGGGGCGCAA CCGGCTCTAC CACGATGGCA AGAACCAGCC AAGCAAAACA 
TACCCAGCCT TTCTGGAACC AGATGAGACA TTCATTGTCC CTGACTCCTT CCTGGTAGCC 
CTGGACATGG ACGACGGGAC TCTGAGCTTC ATTGTGGATG GACAGTACAT GGGAGTGGCT 
TTTCGGGGAC TCAAGGGCAA AAAACTGTAT CCTGTAGTGA GTGCCGTCTG GGGCCACTGT 
GAGATCCGAA TGCGCTACTT GAACGGACTC GATCCCGAGC CGCTGCCGCT CATGGATTTG 
TGCCGTCGCT CGGTGCGCCT GGCCCTGGGG AGGGAGCGCC TGGGGGAGAT CCACACGCTG 
CCGCTGCCGG CTTCCCTCAA GGCCTACCTC CTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079382.2
CDS	328..1149
Translation	MGQKVTGGIKTVDMRDPTYRPLKQELQGLDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFVKEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLGRNRLYHDGKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFRGLKGKKLYPVVSAVWGHCEIRMRYLNGLDPEPLPLMDLCRRSVRLALGRERLGEIHTLPLPASLKAYLLYQ

Target ORF information:

RefSeq Version	NM_025106.4
Organism	Homo sapiens(human)
Definition	Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025106.4

ORF Insert Sequence:

ATGGGTCAGA AGGTCACTGG AGGGATCAAG ACTGTGGACA TGAGGGACCC CACGTACAGG 
CCCCTGAAGC AGGAGCTCCA GGGTCTGGAT TACTGCAAGC CCACCCGGCT GGATCTGCTA 
CTGGACATGC CCCCTGTGTC CTATGATGTC CAGCTGCTGC ATTCATGGAA CAACAACGAC 
CGATCGCTCA ATGTCTTTGT GAAGGAGGAC GACAAGCTCA TCTTTCACCG GCATCCGGTG 
GCCCAGAGCA CGGACGCTAT CAGGGGCAAA GTCGGGTATA CCCGTGGGCT GCACGTGTGG 
CAGATCACGT GGGCCATGAG ACAGCGGGGC ACACACGCCG TGGTGGGGGT GGCGACGGCA 
GACGCCCCCC TGCACTCTGT CGGGTACACA ACCCTCGTGG GGAATAACCA CGAGTCCTGG 
GGCTGGGACT TGGGGCGCAA CCGGCTCTAC CACGATGGCA AGAACCAGCC AAGCAAAACA 
TACCCAGCCT TTCTGGAACC AGATGAGACA TTCATTGTCC CTGACTCCTT CCTGGTAGCC 
CTGGACATGG ACGACGGGAC TCTGAGCTTC ATTGTGGATG GACAGTACAT GGGAGTGGCT 
TTTCGGGGAC TCAAGGGCAA AAAACTGTAT CCTGTAGTGA GTGCCGTCTG GGGCCACTGT 
GAGATCCGAA TGCGCTACTT GAACGGACTC GATCCCGAGC CGCTGCCGCT CATGGATTTG 
TGCCGTCGCT CGGTGCGCCT GGCCCTGGGG AGGGAGCGCC TGGGGGAGAT CCACACGCTG 
CCGCTGCCGG CTTCCCTCAA GGCCTACCTC CTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.
Molecular biology of the cell28(24)3532-3541(2017 Nov)
Okumura F,Joo-Okumura A,Obara K,Petersen A,Nishikimi A,Fukui Y,Nakatsukasa K,Kamura T

SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling.
Cell research27(4)540-558(2017 Apr)
Wang F,Fu X,Chen P,Wu P,Fan X,Li N,Zhu H,Jia TT,Ji H,Wang Z,Wong CC,Hu R,Hui J

SPSB1, a Novel Negative Regulator of the Transforming Growth Factor-? Signaling Pathway Targeting the Type II Receptor.
The Journal of biological chemistry290(29)17894-908(2015 Jul)
Liu S,Nheu T,Luwor R,Nicholson SE,Zhu HJ

PKD1 phosphorylation-dependent degradation of SNAIL by SCF-FBXO11 regulates epithelial-mesenchymal transition and metastasis.
Cancer cell26(3)358-373(2014 Sep)
Zheng H,Shen M,Zha YL,Li W,Wei Y,Blanco MA,Ren G,Zhou T,Storz P,Wang HY,Kang Y

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SPSB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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