ADAMTS20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADAMTS20
Entrez Gene ID	80070
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms	ADAM-TS20,ADAMTS-20,GON-1
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1 motif 20 Names A disintegrin and metalloproteinase with thrombospondin motifs 20 a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 20
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q12
Summary	The protein encoded by this gene is a member of the ADAMTS family of zinc-dependent proteases. The encoded protein has a signal peptide that is cleaved to release the mature peptide, which is secreted and found in the extracellular matrix. This protein may be involved in tissue remodeling. [provided by RefSeq, Sep 2011].
Disorder MIM:	611681

mRNA and Protein(s)

mRNA	Protein	Name
NM_025003.4	NP_079279.3	A disintegrin and metalloproteinase with thrombospondin motifs 20 preproprotein
XM_011538754.2	XP_011537056.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
NM_025003.5	NP_079279.3	A disintegrin and metalloproteinase with thrombospondin motifs 20 preproprotein
XM_017019979.1	XP_016875468.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Adamts20	NP_803180.3
Rattus norvegicus (Norway rat)	Adamts20	XP_006242337.1
	ADAMTS20	NP_001104009.1
Bos taurus (cattle)	ADAMTS20	NP_001193022.1
Gallus gallus (chicken)	ADAMTS20	XP_416037.4
Canis lupus familiaris (dog)	ADAMTS20	XP_852138.3
Xenopus tropicalis (tropical clawed frog)	adamts20	XP_002932868.2
Homo sapiens (human)	ADAMTS20	NP_079279.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association studies in dogs and humans identify ADAMTS20 as a risk variant for cleft lip and palate.
Wolf ZT, Brand HA, Shaffer JR, Leslie EJ, Arzi B, Willet CE, Cox TC, McHenry T, Narayan N, Feingold E, Wang X, Sliskovic S, Karmi N, Safra N, Sanchez C, Deleyiannis FW, Murray JC, Wade CM, Marazita ML, Bannasch DL
PLoS genetics11(3)e1005059(2015 03)

Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease.
Lambert JC, Ibrahim-Verbaas CA, Harold D, Naj AC, Sims R, Bellenguez C, DeStafano AL, Bis JC, Beecham GW, Grenier-Boley B, Russo G, Thorton-Wells TA, Jones N, Smith AV, Chouraki V, Thomas C, Ikram MA, Zelenika D, Vardarajan BN, Kamatani Y, Lin CF, Gerrish A, Schmidt H, Kunkle B, Dunstan ML, Ruiz A, Bihoreau MT, Choi SH, Reitz C, Pasquier F, Cruchaga C, Craig D, Amin N, Berr C, Lopez OL, De Jager PL, Deramecourt V, Johnston JA, Evans D, Lovestone S, Letenneur L, Mor?n FJ, Rubinsztein DC, Eiriksdottir G, Sleegers K, Goate AM, Fi?vet N, Huentelman MW, Gill M, Brown K, Kamboh MI, Keller L, Barberger-Gateau P, McGuiness B, Larson EB, Green R, Myers AJ, Dufouil C, Todd S, Wallon D, Love S, Rogaeva E, Gallacher J, St George-Hyslop P, Clarimon J, Lleo A, Bayer A, Tsuang DW, Yu L, Tsolaki M, Boss? P, Spalletta G, Proitsi P, Collinge J, Sorbi S, Sanchez-Garcia F, Fox NC, Hardy J, Deniz Naranjo MC, Bosco P, Clarke R, Brayne C, Galimberti D, Mancuso M, Matthews F, , , , , Moebus S, Mecocci P, Del Zompo M, Maier W, Hampel H, Pilotto A, Bullido M, Panza F, Caffarra P, Nacmias B, Gilbert JR, Mayhaus M, Lannefelt L, Hakonarson H, Pichler S, Carrasquillo MM, Ingelsson M, Beekly D, Alvarez V, Zou F, Valladares O, Younkin SG, Coto E, Hamilton-Nelson KL, Gu W, Razquin C, Pastor P, Mateo I, Owen MJ, Faber KM, Jonsson PV, Combarros O, O'Donovan MC, Cantwell LB, Soininen H, Blacker D, Mead S, Mosley TH Jr, Bennett DA, Harris TB, Fratiglioni L, Holmes C, de Bruijn RF, Passmore P, Montine TJ, Bettens K, Rotter JI, Brice A, Morgan K, Foroud TM, Kukull WA, Hannequin D, Powell JF, Nalls MA, Ritchie K, Lunetta KL, Kauwe JS, Boerwinkle E, Riemenschneider M, Boada M, Hiltuenen M, Martin ER, Schmidt R, Rujescu D, Wang LS, Dartigues JF, Mayeux R, Tzourio C, Hofman A, N?then MM, Graff C, Psaty BM, Jones L, Haines JL, Holmans PA, Lathrop M, Pericak-Vance MA, Launer LJ, Farrer LA, van Duijn CM, Van Broeckhoven C, Moskvina V, Seshadri S, Williams J, Schellenberg GD, Amouyel P
Nature genetics45(12)1452-8(2013 Dec)

Genome-wide mapping for clinically relevant predictors of lamotrigine- and phenytoin-induced hypersensitivity reactions.
McCormack M, Urban TJ, Shianna KV, Walley N, Pandolfo M, Depondt C, Chaila E, O'Conner GD, Kasperavi?i?t? D, Radtke RA, Heinzen EL, Sisodiya SM, Delanty N, Cavalleri GL
Pharmacogenomics13(4)399-405(2012 Mar)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In summary, this study provides genetic evidence for a role of ADAMTS20 in CL/P development in dogs and as a candidate gene for CL/P development in humans.
Title: Genome-wide association studies in dogs and humans identify ADAMTS20 as a risk variant for cleft lip and palate.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of the OCA2 polymorphism His615Arg with melanin content in east Asian populations: further evidence of convergent evolution of skin pigmentation.

This protein is identified and characterized and its relation to other disintegrins and thrombospondin 1 repeats is examined.
Title: Identification and characterization of ADAMTS-20 defines a novel subfamily of metalloproteinases-disintegrins with multiple thrombospondin-1 repeats and a unique GON domain.

ADAMTS-9 and ADAMTS-20 have an identical modular structure, are distinct in possessing 15 thrombospondin type I repeats and a unique C-terminal domain, and have a similar gene structure
Title: Characterization of ADAMTS-9 and ADAMTS-20 as a distinct ADAMTS subfamily related to Caenorhabditis elegans GON-1.

The following ADAMTS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19605	NM_025003.5 Latest version!	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu72529	XM_011538754.2 Latest version!	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72530	XM_017019979.1 Latest version!	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu19605	NM_025003.4	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADAMTS20 ( NM_025003.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079279.3 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

Price & Availability↑

CloneID	OHu19605
Clone ID Related Accession (Same CDS sequence)	NM_025003.4 , NM_025003.5
Accession Version	NM_025003.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090525.8, AF488804.1, AJ515153.3, AC120104.4 and CN292892.1. This sequence is a reference standard in the RefSeqGene project. On Feb 16, 2017 this sequence version replaced NM_025003.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2153307 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAA ATATATGCAT GCATCTGTGG TGCACAAGCA CAGAAAAGCT TCACAAAGGC 
TGTTTCACTC AACACGTGCC ACCAGCAGAT GGAACAGACT GCGGTCCTGG AATGCATTGC 
CGTCATGGGC TATGTGTAAA CAAAGAAACG GAAACACGTC CTGTAAATGG TGAATGGGGA 
CCATGGGAAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GCGGAATCGA AAGTGCAACC 
AGGCGCTGTA ATCGTCCTGA GCCAAGAAAC GGAGGAAATT ACTGTGTGGG CCGCAGGATG 
AAATTTCGAT CATGTAATAC TGATTCATGT CCAAAAGGCA CACAAGACTT TCGAGAGAAG 
CAGTGCTCTG ATTTTAATGG TAAACATTTG GACATCAGTG GCATTCCCTC TAATGTGAGG 
TGGCTTCCAA GATACAGTGG CATTGGCACA AAGGATCGTT GTAAACTCTA TTGTCAGGTT 
GCTGGAACCA ATTATTTCTA CCTATTGAAG GATATGGTTG AAGATGGTAC TCCTTGTGGA 
ACTGAAACTC ATGACATCTG TGTTCAAGGC CAGTGTATGG CAGCTGGTTG TGATCACGTG 
TTAAACTCCA GTGCCAAGAT AGACAAATGT GGAGTGTGTG GTGGGGACAA CTCTTCATGC 
AAGACAATAA CAGGTGTCTT CAACAGTTCT CATTATGGTT ATAATGTTGT TGTAAAGATT 
CCCGCAGGAG CAACAAACGT TGACATTCGT CAGTACAGCT ATTCTGGACA ACCAGATGAC 
AGTTACCTTG CATTATCTGA CGCTGAAGGG AATTTTCTTT TCAATGGAAA TTTTCTTCTA 
AGTACGTCAA AAAAAGAAAT CAATGTGCAA GGAACAAGAA CTGTTATTGA ATACAGTGGA 
TCAAATAACG CAGTTGAAAG AATTAATAGT ACTAATCGAC AAGAGAAAGA ACTTATTTTG 
CAGGTGTTGT GTGTGGGTAA TTTATACAAC CCTGATGTAC ATTATTCCTT CAATATCCCT 
TTGGAAGAGA GGAGTGACAT GTTCACATGG GACCCCTATG GACCATGGGA AGGCTGTACC 
AAAATGTGTC AAGGTCTTCA GCGAAGAAAC ATAACTTGCA TACATAAGAG TGATCATAGT 
GTTGTGTCTG ATAAAGAATG TGACCACTTG CCACTTCCAT CATTTGTTAC TCAAAGTTGC 
AATACAGACT GTGAACTAAG GTGGCATGTT ATTGGCAAAA GTGAATGTTC ATCCCAATGT 
GGTCAAGGAT ATAGAACCTT GGACATCCAT TGCATGAAGT ATTCCATTCA TGAAGGACAG 
ACTGTTCAAG TTGATGACCA CTACTGTGGT GACCAGCTTA AACCTCCTAC CCAAGAACTA 
TGCCATGGTA ACTGTGTCTT CACAAGATGG CATTATTCAG AATGGTCTCA GTGTTCCAGG 
AGTTGTGGAG GAGGGGAAAG GTCTCGAGAA TCTTATTGTA TGAATAACTT TGGCCATCGT 
CTTGCTGACA ATGAATGCCA AGAACTGTCC CGAGTGACGA GAGAGAATTG CAATGAATTT 
TCCTGTCCCA GTTGGGCTGC TAGTGAATGG AGCGAGTGCC TTGTTACATG TGGTAAAGGA 
ACAAAGCAGC GGCAGGTATG GTGTCAGCTG AATGTAGATC ACTTGAGTGA TGGCTTCTGT 
AATTCAAGTA CCAAACCTGA ATCTCTGAGT CCATGTGAAC TTCATACATG TGCTTCCTGG 
CAAGTAGGAC CATGGGGTCC TTGCACAACC ACATGTGGAC ATGGGTATCA GATGCGAGAT 
GTTAAATGTG TCAATGAGCT AGCTAGTGCA GTGTTAGAGG ACACAGAATG CCATGAAGCT 
AGTCGCCCCA GTGACAGACA GAGCTGTGTA CTTACACCTT GCTCATTTAT TTCTAAACTT 
GAGACCGCTT TATTACCAAC TGTTCTCATA AAAAAGATGG CACAATGGCG ACATGGTTCT 
TGGACCCCAT GCTCCGTATC TTGTGGAAGA GGTACTCAAG CCCGCTATGT AAGCTGTCGT 
GATGCTCTTG ATAGAATAGC AGATGAATCA TATTGTGCCC ACTTACCCCG ACCTGCTGAA 
ATATGGGACT GTTTTACCCC TTGTGGAGAG TGGCAAGCAG GGGATTGGTC ACCCTGTTCA 
GCTTCCTGTG GCCATGGAAA AACAACTCGA CAAGTTTTAT GCATGAACTA CCATCAGCCA 
ATTGATGAGA ATTACTGTGA TCCTGAAGTT CGCCCTTTGA TGGAACAGGA ATGTAGCCTG 
GCAGCCTGCC CTCCTGCACA CAGCCACTTT CCTAGTTCCC CTGTGCAGCC AAGCTATTAT 
CTAAGCACGA ATTTGCCATT AACTCAAAAA CTTGAAGATA ATGAAAATCA GGTGGTCCAT 
CCATCAGTCA GAGGAAACCA GTGGAGAACC GGACCATGGG GATCATGCTC CAGCAGTTGT 
TCTGGAGGTC TTCAGCATAG GGCTGTGGTC TGCCAGGATG AAAATGGACA AAGTGCTAGT 
TACTGCGATG CAGCCTCCAA GCCTCCAGAG TTACAGCAAT GTGGTCCAGG GCCTTGTCCA 
CAGTGGAACT ACGGAAATTG GGGAGAATGT TCACAAACAT GTGGAGGAGG AATAAAATCA 
AGACTTGTAA TATGTCAATT TCCCAATGGC CAAATATTAG AAGATCACAA CTGTGAAATT 
GTAAACAAGC CACCTAGCGT AATACAGTGT CATATGCATG CTTGCCCTGC TGATGTGTCA 
TGGCATCAGG AACCATGGAC ATCGTGCTCA GCTTCTTGTG GTAAAGGTAG AAAGTACCGT 
GAAGTGTTTT GCATTGACCA ATTCCAAAGG AAATTAGAAG ACACAAATTG CAGTCAAGTA 
CAGAAACCTC CAACTCACAA AGCCTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT 
AGCTGGAATG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAGAGGGA TGTATACTGC 
AGACTGAAAG GTGTTGGTCA GGTGGTTGAA GAAATGTGTG ATCAGTCCAC CCGACCTTGT 
TCTCAGAGGC GATGTTGGAG TCAGGACTGT GTGCAGCACA AGGGGATGGA AAGAGGGAGG 
CTGAATTGTT CAACATCATG TGAGAGAAAA GATTCACATC AACGAATGGA GTGCACAGAT 
AACCAAATCA GACAAGTGAA TGAAATAGTC TATAATTCTT CAACCATATC TCTTACATCC 
AAGAATTGCA GGAACCCTCC TTGCAATTAC ATTGTGGTAA CAGCAGACTC ATCACAGTGT 
GCAAACAACT GTGGATTTAG TTACAGACAA AGGATTACAT ATTGCACCGA GATCCCATCT 
ACTAAGAAAC ATAAGCTCCA TCGACTTCGG CCTATAGTTT ATCAAGAATG CCCTGTGGTG 
CCTTCCTCTC AGGTTTACCA ATGCATTAAC AGCTGTTTGC ATTTGGCCAC TTGGAAAGTT 
GGAAAATGGA GCAAGTGCTC AGTGACTTGT GGAATTGGGA TAATGAAGAG ACAAGTGAAA 
TGCATTACCA AACATGGTTT GTCCAGTGAC TTATGTTTAA ACCATTTAAA ACCAGGTGCT 
CAAAAGAAAT GTTATGCCAA TGACTGTAAA TCATTTACCA CCTGCAAAGA AATTCAAGTG 
AAAAACCACA TTAGAAAGGA TGGTGACTAT TACCTTAACA TTAAGGGAAG AATAATAAAG 
ATTTATTGTG CAGACATGTA CTTGGAGAAC CCTAAGGAAT ATTTAACACT GGTCCAAGGT 
GAAGAAAACT TTTCTGAAGT GTATGGCTTT AGACTAAAAA ATCCATATCA ATGTCCTTTT 
AATGGGAGTA GAAGGGAAGA CTGTGAATGT GACAATGGAC ACTTAGCTGC TGGATACACT 
GTTTTCAGCA AAATAAGAAT TGATCTCACT TCCATGCAAA TTAAAACTAC GGACCTTCTT 
TTTTCCAAAA CAATATTTGG AAATGCAGTT CCATTTGCCA CAGCTGGAGA TTGCTACAGT 
GCTTTCAGAT GCCCACAGGG ACAGTTTAGC ATTAATTTGT CAGGAACTGG GATGAAGATA 
TCCAGCACAG CAAAGTGGCT CACTCAGGGG AGTTATACCT CTGTCAGCAT ACGAAGATCA 
GAGGATGGAA CTAGATTTTT CGGCAAATGT GGAGGGTACT GTGGAAAGTG TCTTCCTCAC 
ATGACTACTG GTCTCCCAAT TCAAGTCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079279.3
CDS	51..5783
Translation	MWVAKWLTGLLYHLSLFITRSWEVDFHPRQEALVRTLTSYEVVIPERVNEFGEVFPQSHHFSRQKRSSEALEPMPFRTHYRFTAYGQLFQLNLTADASFLAAGYTEVHLGTPERGAWESDAGPSDLRHCFYRGQVNSQEDYKAVVSLCGGLTGTFKGQNGEYFLEPIMKADGNEYEDGHNKPHLIYRQDLNNSFLQTLKYCSVSESQIKETSLPFHTYSNMNEDLNVMKERVLGHTSKNVPLKDERRHSRKKRLISYPRYIEIMVTADAKVVSAHGSNLQNYILTLMSIVATIYKDPSIGNLIHIVVVKLVMIHREEEGPVINFDGATTLKNFCSWQQTQNDLDDVHPSHHDTAVLITREDICSSKEKCNMLGLSYLGTICDPLQSCFINEEKGLISAFTIAHELGHTLGVQHDDNPRCKEMKVTKYHVMAPALSFHMSPWSWSNCSRKYVTEFLDTGYGECLLDKPDEEIYNLPSELPGSRYDGNKQCELAFGPGSQMCPHINICMHLWCTSTEKLHKGCFTQHVPPADGTDCGPGMHCRHGLCVNKETETRPVNGEWGPWEPYSSCSRTCGGGIESATRRCNRPEPRNGGNYCVGRRMKFRSCNTDSCPKGTQDFREKQCSDFNGKHLDISGIPSNVRWLPRYSGIGTKDRCKLYCQVAGTNYFYLLKDMVEDGTPCGTETHDICVQGQCMAAGCDHVLNSSAKIDKCGVCGGDNSSCKTITGVFNSSHYGYNVVVKIPAGATNVDIRQYSYSGQPDDSYLALSDAEGNFLFNGNFLLSTSKKEINVQGTRTVIEYSGSNNAVERINSTNRQEKELILQVLCVGNLYNPDVHYSFNIPLEERSDMFTWDPYGPWEGCTKMCQGLQRRNITCIHKSDHSVVSDKECDHLPLPSFVTQSCNTDCELRWHVIGKSECSSQCGQGYRTLDIHCMKYSIHEGQTVQVDDHYCGDQLKPPTQELCHGNCVFTRWHYSEWSQCSRSCGGGERSRESYCMNNFGHRLADNECQELSRVTRENCNEFSCPSWAASEWSECLVTCGKGTKQRQVWCQLNVDHLSDGFCNSSTKPESLSPCELHTCASWQVGPWGPCTTTCGHGYQMRDVKCVNELASAVLEDTECHEASRPSDRQSCVLTPCSFISKLETALLPTVLIKKMAQWRHGSWTPCSVSCGRGTQARYVSCRDALDRIADESYCAHLPRPAEIWDCFTPCGEWQAGDWSPCSASCGHGKTTRQVLCMNYHQPIDENYCDPEVRPLMEQECSLAACPPAHSHFPSSPVQPSYYLSTNLPLTQKLEDNENQVVHPSVRGNQWRTGPWGSCSSSCSGGLQHRAVVCQDENGQSASYCDAASKPPELQQCGPGPCPQWNYGNWGECSQTCGGGIKSRLVICQFPNGQILEDHNCEIVNKPPSVIQCHMHACPADVSWHQEPWTSCSASCGKGRKYREVFCIDQFQRKLEDTNCSQVQKPPTHKACRSVRCPSWKANSWNECSVTCGSGVQQRDVYCRLKGVGQVVEEMCDQSTRPCSQRRCWSQDCVQHKGMERGRLNCSTSCERKDSHQRMECTDNQIRQVNEIVYNSSTISLTSKNCRNPPCNYIVVTADSSQCANNCGFSYRQRITYCTEIPSTKKHKLHRLRPIVYQECPVVPSSQVYQCINSCLHLATWKVGKWSKCSVTCGIGIMKRQVKCITKHGLSSDLCLNHLKPGAQKKCYANDCKSFTTCKEIQVKNHIRKDGDYYLNIKGRIIKIYCADMYLENPKEYLTLVQGEENFSEVYGFRLKNPYQCPFNGSRREDCECDNGHLAAGYTVFSKIRIDLTSMQIKTTDLLFSKTIFGNAVPFATAGDCYSAFRCPQGQFSINLSGTGMKISSTAKWLTQGSYTSVSIRRSEDGTRFFGKCGGYCGKCLPHMTTGLPIQVI

Target ORF information:

RefSeq Version	NM_025003.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025003.4

ORF Insert Sequence:

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAA ATATATGCAT GCATCTGTGG TGCACAAGCA CAGAAAAGCT TCACAAAGGC 
TGTTTCACTC AACACGTGCC ACCAGCAGAT GGAACAGACT GCGGTCCTGG AATGCATTGC 
CGTCATGGGC TATGTGTAAA CAAAGAAACG GAAACACGTC CTGTAAATGG TGAATGGGGA 
CCATGGGAAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GCGGAATCGA AAGTGCAACC 
AGGCGCTGTA ATCGTCCTGA GCCAAGAAAC GGAGGAAATT ACTGTGTGGG CCGCAGGATG 
AAATTTCGAT CATGTAATAC TGATTCATGT CCAAAAGGCA CACAAGACTT TCGAGAGAAG 
CAGTGCTCTG ATTTTAATGG TAAACATTTG GACATCAGTG GCATTCCCTC TAATGTGAGG 
TGGCTTCCAA GATACAGTGG CATTGGCACA AAGGATCGTT GTAAACTCTA TTGTCAGGTT 
GCTGGAACCA ATTATTTCTA CCTATTGAAG GATATGGTTG AAGATGGTAC TCCTTGTGGA 
ACTGAAACTC ATGACATCTG TGTTCAAGGC CAGTGTATGG CAGCTGGTTG TGATCACGTG 
TTAAACTCCA GTGCCAAGAT AGACAAATGT GGAGTGTGTG GTGGGGACAA CTCTTCATGC 
AAGACAATAA CAGGTGTCTT CAACAGTTCT CATTATGGTT ATAATGTTGT TGTAAAGATT 
CCCGCAGGAG CAACAAACGT TGACATTCGT CAGTACAGCT ATTCTGGACA ACCAGATGAC 
AGTTACCTTG CATTATCTGA CGCTGAAGGG AATTTTCTTT TCAATGGAAA TTTTCTTCTA 
AGTACGTCAA AAAAAGAAAT CAATGTGCAA GGAACAAGAA CTGTTATTGA ATACAGTGGA 
TCAAATAACG CAGTTGAAAG AATTAATAGT ACTAATCGAC AAGAGAAAGA ACTTATTTTG 
CAGGTGTTGT GTGTGGGTAA TTTATACAAC CCTGATGTAC ATTATTCCTT CAATATCCCT 
TTGGAAGAGA GGAGTGACAT GTTCACATGG GACCCCTATG GACCATGGGA AGGCTGTACC 
AAAATGTGTC AAGGTCTTCA GCGAAGAAAC ATAACTTGCA TACATAAGAG TGATCATAGT 
GTTGTGTCTG ATAAAGAATG TGACCACTTG CCACTTCCAT CATTTGTTAC TCAAAGTTGC 
AATACAGACT GTGAACTAAG GTGGCATGTT ATTGGCAAAA GTGAATGTTC ATCCCAATGT 
GGTCAAGGAT ATAGAACCTT GGACATCCAT TGCATGAAGT ATTCCATTCA TGAAGGACAG 
ACTGTTCAAG TTGATGACCA CTACTGTGGT GACCAGCTTA AACCTCCTAC CCAAGAACTA 
TGCCATGGTA ACTGTGTCTT CACAAGATGG CATTATTCAG AATGGTCTCA GTGTTCCAGG 
AGTTGTGGAG GAGGGGAAAG GTCTCGAGAA TCTTATTGTA TGAATAACTT TGGCCATCGT 
CTTGCTGACA ATGAATGCCA AGAACTGTCC CGAGTGACGA GAGAGAATTG CAATGAATTT 
TCCTGTCCCA GTTGGGCTGC TAGTGAATGG AGCGAGTGCC TTGTTACATG TGGTAAAGGA 
ACAAAGCAGC GGCAGGTATG GTGTCAGCTG AATGTAGATC ACTTGAGTGA TGGCTTCTGT 
AATTCAAGTA CCAAACCTGA ATCTCTGAGT CCATGTGAAC TTCATACATG TGCTTCCTGG 
CAAGTAGGAC CATGGGGTCC TTGCACAACC ACATGTGGAC ATGGGTATCA GATGCGAGAT 
GTTAAATGTG TCAATGAGCT AGCTAGTGCA GTGTTAGAGG ACACAGAATG CCATGAAGCT 
AGTCGCCCCA GTGACAGACA GAGCTGTGTA CTTACACCTT GCTCATTTAT TTCTAAACTT 
GAGACCGCTT TATTACCAAC TGTTCTCATA AAAAAGATGG CACAATGGCG ACATGGTTCT 
TGGACCCCAT GCTCCGTATC TTGTGGAAGA GGTACTCAAG CCCGCTATGT AAGCTGTCGT 
GATGCTCTTG ATAGAATAGC AGATGAATCA TATTGTGCCC ACTTACCCCG ACCTGCTGAA 
ATATGGGACT GTTTTACCCC TTGTGGAGAG TGGCAAGCAG GGGATTGGTC ACCCTGTTCA 
GCTTCCTGTG GCCATGGAAA AACAACTCGA CAAGTTTTAT GCATGAACTA CCATCAGCCA 
ATTGATGAGA ATTACTGTGA TCCTGAAGTT CGCCCTTTGA TGGAACAGGA ATGTAGCCTG 
GCAGCCTGCC CTCCTGCACA CAGCCACTTT CCTAGTTCCC CTGTGCAGCC AAGCTATTAT 
CTAAGCACGA ATTTGCCATT AACTCAAAAA CTTGAAGATA ATGAAAATCA GGTGGTCCAT 
CCATCAGTCA GAGGAAACCA GTGGAGAACC GGACCATGGG GATCATGCTC CAGCAGTTGT 
TCTGGAGGTC TTCAGCATAG GGCTGTGGTC TGCCAGGATG AAAATGGACA AAGTGCTAGT 
TACTGCGATG CAGCCTCCAA GCCTCCAGAG TTACAGCAAT GTGGTCCAGG GCCTTGTCCA 
CAGTGGAACT ACGGAAATTG GGGAGAATGT TCACAAACAT GTGGAGGAGG AATAAAATCA 
AGACTTGTAA TATGTCAATT TCCCAATGGC CAAATATTAG AAGATCACAA CTGTGAAATT 
GTAAACAAGC CACCTAGCGT AATACAGTGT CATATGCATG CTTGCCCTGC TGATGTGTCA 
TGGCATCAGG AACCATGGAC ATCGTGCTCA GCTTCTTGTG GTAAAGGTAG AAAGTACCGT 
GAAGTGTTTT GCATTGACCA ATTCCAAAGG AAATTAGAAG ACACAAATTG CAGTCAAGTA 
CAGAAACCTC CAACTCACAA AGCCTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT 
AGCTGGAATG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAGAGGGA TGTATACTGC 
AGACTGAAAG GTGTTGGTCA GGTGGTTGAA GAAATGTGTG ATCAGTCCAC CCGACCTTGT 
TCTCAGAGGC GATGTTGGAG TCAGGACTGT GTGCAGCACA AGGGGATGGA AAGAGGGAGG 
CTGAATTGTT CAACATCATG TGAGAGAAAA GATTCACATC AACGAATGGA GTGCACAGAT 
AACCAAATCA GACAAGTGAA TGAAATAGTC TATAATTCTT CAACCATATC TCTTACATCC 
AAGAATTGCA GGAACCCTCC TTGCAATTAC ATTGTGGTAA CAGCAGACTC ATCACAGTGT 
GCAAACAACT GTGGATTTAG TTACAGACAA AGGATTACAT ATTGCACCGA GATCCCATCT 
ACTAAGAAAC ATAAGCTCCA TCGACTTCGG CCTATAGTTT ATCAAGAATG CCCTGTGGTG 
CCTTCCTCTC AGGTTTACCA ATGCATTAAC AGCTGTTTGC ATTTGGCCAC TTGGAAAGTT 
GGAAAATGGA GCAAGTGCTC AGTGACTTGT GGAATTGGGA TAATGAAGAG ACAAGTGAAA 
TGCATTACCA AACATGGTTT GTCCAGTGAC TTATGTTTAA ACCATTTAAA ACCAGGTGCT 
CAAAAGAAAT GTTATGCCAA TGACTGTAAA TCATTTACCA CCTGCAAAGA AATTCAAGTG 
AAAAACCACA TTAGAAAGGA TGGTGACTAT TACCTTAACA TTAAGGGAAG AATAATAAAG 
ATTTATTGTG CAGACATGTA CTTGGAGAAC CCTAAGGAAT ATTTAACACT GGTCCAAGGT 
GAAGAAAACT TTTCTGAAGT GTATGGCTTT AGACTAAAAA ATCCATATCA ATGTCCTTTT 
AATGGGAGTA GAAGGGAAGA CTGTGAATGT GACAATGGAC ACTTAGCTGC TGGATACACT 
GTTTTCAGCA AAATAAGAAT TGATCTCACT TCCATGCAAA TTAAAACTAC GGACCTTCTT 
TTTTCCAAAA CAATATTTGG AAATGCAGTT CCATTTGCCA CAGCTGGAGA TTGCTACAGT 
GCTTTCAGAT GCCCACAGGG ACAGTTTAGC ATTAATTTGT CAGGAACTGG GATGAAGATA 
TCCAGCACAG CAAAGTGGCT CACTCAGGGG AGTTATACCT CTGTCAGCAT ACGAAGATCA 
GAGGATGGAA CTAGATTTTT CGGCAAATGT GGAGGGTACT GTGGAAAGTG TCTTCCTCAC 
ATGACTACTG GTCTCCCAAT TCAAGTCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( NM_025003.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_079279.3 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

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CloneID	OHu19605
Clone ID Related Accession (Same CDS sequence)	NM_025003.4 , NM_025003.5
Accession Version	NM_025003.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090525.8, AF488804.1, AJ515153.3, AC120104.4 and CN292892.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_025003.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2153307 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389420.8/ ENSP00000374071.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END##

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAA ATATATGCAT GCATCTGTGG TGCACAAGCA CAGAAAAGCT TCACAAAGGC 
TGTTTCACTC AACACGTGCC ACCAGCAGAT GGAACAGACT GCGGTCCTGG AATGCATTGC 
CGTCATGGGC TATGTGTAAA CAAAGAAACG GAAACACGTC CTGTAAATGG TGAATGGGGA 
CCATGGGAAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GCGGAATCGA AAGTGCAACC 
AGGCGCTGTA ATCGTCCTGA GCCAAGAAAC GGAGGAAATT ACTGTGTGGG CCGCAGGATG 
AAATTTCGAT CATGTAATAC TGATTCATGT CCAAAAGGCA CACAAGACTT TCGAGAGAAG 
CAGTGCTCTG ATTTTAATGG TAAACATTTG GACATCAGTG GCATTCCCTC TAATGTGAGG 
TGGCTTCCAA GATACAGTGG CATTGGCACA AAGGATCGTT GTAAACTCTA TTGTCAGGTT 
GCTGGAACCA ATTATTTCTA CCTATTGAAG GATATGGTTG AAGATGGTAC TCCTTGTGGA 
ACTGAAACTC ATGACATCTG TGTTCAAGGC CAGTGTATGG CAGCTGGTTG TGATCACGTG 
TTAAACTCCA GTGCCAAGAT AGACAAATGT GGAGTGTGTG GTGGGGACAA CTCTTCATGC 
AAGACAATAA CAGGTGTCTT CAACAGTTCT CATTATGGTT ATAATGTTGT TGTAAAGATT 
CCCGCAGGAG CAACAAACGT TGACATTCGT CAGTACAGCT ATTCTGGACA ACCAGATGAC 
AGTTACCTTG CATTATCTGA CGCTGAAGGG AATTTTCTTT TCAATGGAAA TTTTCTTCTA 
AGTACGTCAA AAAAAGAAAT CAATGTGCAA GGAACAAGAA CTGTTATTGA ATACAGTGGA 
TCAAATAACG CAGTTGAAAG AATTAATAGT ACTAATCGAC AAGAGAAAGA ACTTATTTTG 
CAGGTGTTGT GTGTGGGTAA TTTATACAAC CCTGATGTAC ATTATTCCTT CAATATCCCT 
TTGGAAGAGA GGAGTGACAT GTTCACATGG GACCCCTATG GACCATGGGA AGGCTGTACC 
AAAATGTGTC AAGGTCTTCA GCGAAGAAAC ATAACTTGCA TACATAAGAG TGATCATAGT 
GTTGTGTCTG ATAAAGAATG TGACCACTTG CCACTTCCAT CATTTGTTAC TCAAAGTTGC 
AATACAGACT GTGAACTAAG GTGGCATGTT ATTGGCAAAA GTGAATGTTC ATCCCAATGT 
GGTCAAGGAT ATAGAACCTT GGACATCCAT TGCATGAAGT ATTCCATTCA TGAAGGACAG 
ACTGTTCAAG TTGATGACCA CTACTGTGGT GACCAGCTTA AACCTCCTAC CCAAGAACTA 
TGCCATGGTA ACTGTGTCTT CACAAGATGG CATTATTCAG AATGGTCTCA GTGTTCCAGG 
AGTTGTGGAG GAGGGGAAAG GTCTCGAGAA TCTTATTGTA TGAATAACTT TGGCCATCGT 
CTTGCTGACA ATGAATGCCA AGAACTGTCC CGAGTGACGA GAGAGAATTG CAATGAATTT 
TCCTGTCCCA GTTGGGCTGC TAGTGAATGG AGCGAGTGCC TTGTTACATG TGGTAAAGGA 
ACAAAGCAGC GGCAGGTATG GTGTCAGCTG AATGTAGATC ACTTGAGTGA TGGCTTCTGT 
AATTCAAGTA CCAAACCTGA ATCTCTGAGT CCATGTGAAC TTCATACATG TGCTTCCTGG 
CAAGTAGGAC CATGGGGTCC TTGCACAACC ACATGTGGAC ATGGGTATCA GATGCGAGAT 
GTTAAATGTG TCAATGAGCT AGCTAGTGCA GTGTTAGAGG ACACAGAATG CCATGAAGCT 
AGTCGCCCCA GTGACAGACA GAGCTGTGTA CTTACACCTT GCTCATTTAT TTCTAAACTT 
GAGACCGCTT TATTACCAAC TGTTCTCATA AAAAAGATGG CACAATGGCG ACATGGTTCT 
TGGACCCCAT GCTCCGTATC TTGTGGAAGA GGTACTCAAG CCCGCTATGT AAGCTGTCGT 
GATGCTCTTG ATAGAATAGC AGATGAATCA TATTGTGCCC ACTTACCCCG ACCTGCTGAA 
ATATGGGACT GTTTTACCCC TTGTGGAGAG TGGCAAGCAG GGGATTGGTC ACCCTGTTCA 
GCTTCCTGTG GCCATGGAAA AACAACTCGA CAAGTTTTAT GCATGAACTA CCATCAGCCA 
ATTGATGAGA ATTACTGTGA TCCTGAAGTT CGCCCTTTGA TGGAACAGGA ATGTAGCCTG 
GCAGCCTGCC CTCCTGCACA CAGCCACTTT CCTAGTTCCC CTGTGCAGCC AAGCTATTAT 
CTAAGCACGA ATTTGCCATT AACTCAAAAA CTTGAAGATA ATGAAAATCA GGTGGTCCAT 
CCATCAGTCA GAGGAAACCA GTGGAGAACC GGACCATGGG GATCATGCTC CAGCAGTTGT 
TCTGGAGGTC TTCAGCATAG GGCTGTGGTC TGCCAGGATG AAAATGGACA AAGTGCTAGT 
TACTGCGATG CAGCCTCCAA GCCTCCAGAG TTACAGCAAT GTGGTCCAGG GCCTTGTCCA 
CAGTGGAACT ACGGAAATTG GGGAGAATGT TCACAAACAT GTGGAGGAGG AATAAAATCA 
AGACTTGTAA TATGTCAATT TCCCAATGGC CAAATATTAG AAGATCACAA CTGTGAAATT 
GTAAACAAGC CACCTAGCGT AATACAGTGT CATATGCATG CTTGCCCTGC TGATGTGTCA 
TGGCATCAGG AACCATGGAC ATCGTGCTCA GCTTCTTGTG GTAAAGGTAG AAAGTACCGT 
GAAGTGTTTT GCATTGACCA ATTCCAAAGG AAATTAGAAG ACACAAATTG CAGTCAAGTA 
CAGAAACCTC CAACTCACAA AGCCTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT 
AGCTGGAATG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAGAGGGA TGTATACTGC 
AGACTGAAAG GTGTTGGTCA GGTGGTTGAA GAAATGTGTG ATCAGTCCAC CCGACCTTGT 
TCTCAGAGGC GATGTTGGAG TCAGGACTGT GTGCAGCACA AGGGGATGGA AAGAGGGAGG 
CTGAATTGTT CAACATCATG TGAGAGAAAA GATTCACATC AACGAATGGA GTGCACAGAT 
AACCAAATCA GACAAGTGAA TGAAATAGTC TATAATTCTT CAACCATATC TCTTACATCC 
AAGAATTGCA GGAACCCTCC TTGCAATTAC ATTGTGGTAA CAGCAGACTC ATCACAGTGT 
GCAAACAACT GTGGATTTAG TTACAGACAA AGGATTACAT ATTGCACCGA GATCCCATCT 
ACTAAGAAAC ATAAGCTCCA TCGACTTCGG CCTATAGTTT ATCAAGAATG CCCTGTGGTG 
CCTTCCTCTC AGGTTTACCA ATGCATTAAC AGCTGTTTGC ATTTGGCCAC TTGGAAAGTT 
GGAAAATGGA GCAAGTGCTC AGTGACTTGT GGAATTGGGA TAATGAAGAG ACAAGTGAAA 
TGCATTACCA AACATGGTTT GTCCAGTGAC TTATGTTTAA ACCATTTAAA ACCAGGTGCT 
CAAAAGAAAT GTTATGCCAA TGACTGTAAA TCATTTACCA CCTGCAAAGA AATTCAAGTG 
AAAAACCACA TTAGAAAGGA TGGTGACTAT TACCTTAACA TTAAGGGAAG AATAATAAAG 
ATTTATTGTG CAGACATGTA CTTGGAGAAC CCTAAGGAAT ATTTAACACT GGTCCAAGGT 
GAAGAAAACT TTTCTGAAGT GTATGGCTTT AGACTAAAAA ATCCATATCA ATGTCCTTTT 
AATGGGAGTA GAAGGGAAGA CTGTGAATGT GACAATGGAC ACTTAGCTGC TGGATACACT 
GTTTTCAGCA AAATAAGAAT TGATCTCACT TCCATGCAAA TTAAAACTAC GGACCTTCTT 
TTTTCCAAAA CAATATTTGG AAATGCAGTT CCATTTGCCA CAGCTGGAGA TTGCTACAGT 
GCTTTCAGAT GCCCACAGGG ACAGTTTAGC ATTAATTTGT CAGGAACTGG GATGAAGATA 
TCCAGCACAG CAAAGTGGCT CACTCAGGGG AGTTATACCT CTGTCAGCAT ACGAAGATCA 
GAGGATGGAA CTAGATTTTT CGGCAAATGT GGAGGGTACT GTGGAAAGTG TCTTCCTCAC 
ATGACTACTG GTCTCCCAAT TCAAGTCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079279.3
CDS	283..6015
Translation	MWVAKWLTGLLYHLSLFITRSWEVDFHPRQEALVRTLTSYEVVIPERVNEFGEVFPQSHHFSRQKRSSEALEPMPFRTHYRFTAYGQLFQLNLTADASFLAAGYTEVHLGTPERGAWESDAGPSDLRHCFYRGQVNSQEDYKAVVSLCGGLTGTFKGQNGEYFLEPIMKADGNEYEDGHNKPHLIYRQDLNNSFLQTLKYCSVSESQIKETSLPFHTYSNMNEDLNVMKERVLGHTSKNVPLKDERRHSRKKRLISYPRYIEIMVTADAKVVSAHGSNLQNYILTLMSIVATIYKDPSIGNLIHIVVVKLVMIHREEEGPVINFDGATTLKNFCSWQQTQNDLDDVHPSHHDTAVLITREDICSSKEKCNMLGLSYLGTICDPLQSCFINEEKGLISAFTIAHELGHTLGVQHDDNPRCKEMKVTKYHVMAPALSFHMSPWSWSNCSRKYVTEFLDTGYGECLLDKPDEEIYNLPSELPGSRYDGNKQCELAFGPGSQMCPHINICMHLWCTSTEKLHKGCFTQHVPPADGTDCGPGMHCRHGLCVNKETETRPVNGEWGPWEPYSSCSRTCGGGIESATRRCNRPEPRNGGNYCVGRRMKFRSCNTDSCPKGTQDFREKQCSDFNGKHLDISGIPSNVRWLPRYSGIGTKDRCKLYCQVAGTNYFYLLKDMVEDGTPCGTETHDICVQGQCMAAGCDHVLNSSAKIDKCGVCGGDNSSCKTITGVFNSSHYGYNVVVKIPAGATNVDIRQYSYSGQPDDSYLALSDAEGNFLFNGNFLLSTSKKEINVQGTRTVIEYSGSNNAVERINSTNRQEKELILQVLCVGNLYNPDVHYSFNIPLEERSDMFTWDPYGPWEGCTKMCQGLQRRNITCIHKSDHSVVSDKECDHLPLPSFVTQSCNTDCELRWHVIGKSECSSQCGQGYRTLDIHCMKYSIHEGQTVQVDDHYCGDQLKPPTQELCHGNCVFTRWHYSEWSQCSRSCGGGERSRESYCMNNFGHRLADNECQELSRVTRENCNEFSCPSWAASEWSECLVTCGKGTKQRQVWCQLNVDHLSDGFCNSSTKPESLSPCELHTCASWQVGPWGPCTTTCGHGYQMRDVKCVNELASAVLEDTECHEASRPSDRQSCVLTPCSFISKLETALLPTVLIKKMAQWRHGSWTPCSVSCGRGTQARYVSCRDALDRIADESYCAHLPRPAEIWDCFTPCGEWQAGDWSPCSASCGHGKTTRQVLCMNYHQPIDENYCDPEVRPLMEQECSLAACPPAHSHFPSSPVQPSYYLSTNLPLTQKLEDNENQVVHPSVRGNQWRTGPWGSCSSSCSGGLQHRAVVCQDENGQSASYCDAASKPPELQQCGPGPCPQWNYGNWGECSQTCGGGIKSRLVICQFPNGQILEDHNCEIVNKPPSVIQCHMHACPADVSWHQEPWTSCSASCGKGRKYREVFCIDQFQRKLEDTNCSQVQKPPTHKACRSVRCPSWKANSWNECSVTCGSGVQQRDVYCRLKGVGQVVEEMCDQSTRPCSQRRCWSQDCVQHKGMERGRLNCSTSCERKDSHQRMECTDNQIRQVNEIVYNSSTISLTSKNCRNPPCNYIVVTADSSQCANNCGFSYRQRITYCTEIPSTKKHKLHRLRPIVYQECPVVPSSQVYQCINSCLHLATWKVGKWSKCSVTCGIGIMKRQVKCITKHGLSSDLCLNHLKPGAQKKCYANDCKSFTTCKEIQVKNHIRKDGDYYLNIKGRIIKIYCADMYLENPKEYLTLVQGEENFSEVYGFRLKNPYQCPFNGSRREDCECDNGHLAAGYTVFSKIRIDLTSMQIKTTDLLFSKTIFGNAVPFATAGDCYSAFRCPQGQFSINLSGTGMKISSTAKWLTQGSYTSVSIRRSEDGTRFFGKCGGYCGKCLPHMTTGLPIQVI

Target ORF information:

RefSeq Version	NM_025003.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025003.5

ORF Insert Sequence:

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAA ATATATGCAT GCATCTGTGG TGCACAAGCA CAGAAAAGCT TCACAAAGGC 
TGTTTCACTC AACACGTGCC ACCAGCAGAT GGAACAGACT GCGGTCCTGG AATGCATTGC 
CGTCATGGGC TATGTGTAAA CAAAGAAACG GAAACACGTC CTGTAAATGG TGAATGGGGA 
CCATGGGAAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GCGGAATCGA AAGTGCAACC 
AGGCGCTGTA ATCGTCCTGA GCCAAGAAAC GGAGGAAATT ACTGTGTGGG CCGCAGGATG 
AAATTTCGAT CATGTAATAC TGATTCATGT CCAAAAGGCA CACAAGACTT TCGAGAGAAG 
CAGTGCTCTG ATTTTAATGG TAAACATTTG GACATCAGTG GCATTCCCTC TAATGTGAGG 
TGGCTTCCAA GATACAGTGG CATTGGCACA AAGGATCGTT GTAAACTCTA TTGTCAGGTT 
GCTGGAACCA ATTATTTCTA CCTATTGAAG GATATGGTTG AAGATGGTAC TCCTTGTGGA 
ACTGAAACTC ATGACATCTG TGTTCAAGGC CAGTGTATGG CAGCTGGTTG TGATCACGTG 
TTAAACTCCA GTGCCAAGAT AGACAAATGT GGAGTGTGTG GTGGGGACAA CTCTTCATGC 
AAGACAATAA CAGGTGTCTT CAACAGTTCT CATTATGGTT ATAATGTTGT TGTAAAGATT 
CCCGCAGGAG CAACAAACGT TGACATTCGT CAGTACAGCT ATTCTGGACA ACCAGATGAC 
AGTTACCTTG CATTATCTGA CGCTGAAGGG AATTTTCTTT TCAATGGAAA TTTTCTTCTA 
AGTACGTCAA AAAAAGAAAT CAATGTGCAA GGAACAAGAA CTGTTATTGA ATACAGTGGA 
TCAAATAACG CAGTTGAAAG AATTAATAGT ACTAATCGAC AAGAGAAAGA ACTTATTTTG 
CAGGTGTTGT GTGTGGGTAA TTTATACAAC CCTGATGTAC ATTATTCCTT CAATATCCCT 
TTGGAAGAGA GGAGTGACAT GTTCACATGG GACCCCTATG GACCATGGGA AGGCTGTACC 
AAAATGTGTC AAGGTCTTCA GCGAAGAAAC ATAACTTGCA TACATAAGAG TGATCATAGT 
GTTGTGTCTG ATAAAGAATG TGACCACTTG CCACTTCCAT CATTTGTTAC TCAAAGTTGC 
AATACAGACT GTGAACTAAG GTGGCATGTT ATTGGCAAAA GTGAATGTTC ATCCCAATGT 
GGTCAAGGAT ATAGAACCTT GGACATCCAT TGCATGAAGT ATTCCATTCA TGAAGGACAG 
ACTGTTCAAG TTGATGACCA CTACTGTGGT GACCAGCTTA AACCTCCTAC CCAAGAACTA 
TGCCATGGTA ACTGTGTCTT CACAAGATGG CATTATTCAG AATGGTCTCA GTGTTCCAGG 
AGTTGTGGAG GAGGGGAAAG GTCTCGAGAA TCTTATTGTA TGAATAACTT TGGCCATCGT 
CTTGCTGACA ATGAATGCCA AGAACTGTCC CGAGTGACGA GAGAGAATTG CAATGAATTT 
TCCTGTCCCA GTTGGGCTGC TAGTGAATGG AGCGAGTGCC TTGTTACATG TGGTAAAGGA 
ACAAAGCAGC GGCAGGTATG GTGTCAGCTG AATGTAGATC ACTTGAGTGA TGGCTTCTGT 
AATTCAAGTA CCAAACCTGA ATCTCTGAGT CCATGTGAAC TTCATACATG TGCTTCCTGG 
CAAGTAGGAC CATGGGGTCC TTGCACAACC ACATGTGGAC ATGGGTATCA GATGCGAGAT 
GTTAAATGTG TCAATGAGCT AGCTAGTGCA GTGTTAGAGG ACACAGAATG CCATGAAGCT 
AGTCGCCCCA GTGACAGACA GAGCTGTGTA CTTACACCTT GCTCATTTAT TTCTAAACTT 
GAGACCGCTT TATTACCAAC TGTTCTCATA AAAAAGATGG CACAATGGCG ACATGGTTCT 
TGGACCCCAT GCTCCGTATC TTGTGGAAGA GGTACTCAAG CCCGCTATGT AAGCTGTCGT 
GATGCTCTTG ATAGAATAGC AGATGAATCA TATTGTGCCC ACTTACCCCG ACCTGCTGAA 
ATATGGGACT GTTTTACCCC TTGTGGAGAG TGGCAAGCAG GGGATTGGTC ACCCTGTTCA 
GCTTCCTGTG GCCATGGAAA AACAACTCGA CAAGTTTTAT GCATGAACTA CCATCAGCCA 
ATTGATGAGA ATTACTGTGA TCCTGAAGTT CGCCCTTTGA TGGAACAGGA ATGTAGCCTG 
GCAGCCTGCC CTCCTGCACA CAGCCACTTT CCTAGTTCCC CTGTGCAGCC AAGCTATTAT 
CTAAGCACGA ATTTGCCATT AACTCAAAAA CTTGAAGATA ATGAAAATCA GGTGGTCCAT 
CCATCAGTCA GAGGAAACCA GTGGAGAACC GGACCATGGG GATCATGCTC CAGCAGTTGT 
TCTGGAGGTC TTCAGCATAG GGCTGTGGTC TGCCAGGATG AAAATGGACA AAGTGCTAGT 
TACTGCGATG CAGCCTCCAA GCCTCCAGAG TTACAGCAAT GTGGTCCAGG GCCTTGTCCA 
CAGTGGAACT ACGGAAATTG GGGAGAATGT TCACAAACAT GTGGAGGAGG AATAAAATCA 
AGACTTGTAA TATGTCAATT TCCCAATGGC CAAATATTAG AAGATCACAA CTGTGAAATT 
GTAAACAAGC CACCTAGCGT AATACAGTGT CATATGCATG CTTGCCCTGC TGATGTGTCA 
TGGCATCAGG AACCATGGAC ATCGTGCTCA GCTTCTTGTG GTAAAGGTAG AAAGTACCGT 
GAAGTGTTTT GCATTGACCA ATTCCAAAGG AAATTAGAAG ACACAAATTG CAGTCAAGTA 
CAGAAACCTC CAACTCACAA AGCCTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT 
AGCTGGAATG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAGAGGGA TGTATACTGC 
AGACTGAAAG GTGTTGGTCA GGTGGTTGAA GAAATGTGTG ATCAGTCCAC CCGACCTTGT 
TCTCAGAGGC GATGTTGGAG TCAGGACTGT GTGCAGCACA AGGGGATGGA AAGAGGGAGG 
CTGAATTGTT CAACATCATG TGAGAGAAAA GATTCACATC AACGAATGGA GTGCACAGAT 
AACCAAATCA GACAAGTGAA TGAAATAGTC TATAATTCTT CAACCATATC TCTTACATCC 
AAGAATTGCA GGAACCCTCC TTGCAATTAC ATTGTGGTAA CAGCAGACTC ATCACAGTGT 
GCAAACAACT GTGGATTTAG TTACAGACAA AGGATTACAT ATTGCACCGA GATCCCATCT 
ACTAAGAAAC ATAAGCTCCA TCGACTTCGG CCTATAGTTT ATCAAGAATG CCCTGTGGTG 
CCTTCCTCTC AGGTTTACCA ATGCATTAAC AGCTGTTTGC ATTTGGCCAC TTGGAAAGTT 
GGAAAATGGA GCAAGTGCTC AGTGACTTGT GGAATTGGGA TAATGAAGAG ACAAGTGAAA 
TGCATTACCA AACATGGTTT GTCCAGTGAC TTATGTTTAA ACCATTTAAA ACCAGGTGCT 
CAAAAGAAAT GTTATGCCAA TGACTGTAAA TCATTTACCA CCTGCAAAGA AATTCAAGTG 
AAAAACCACA TTAGAAAGGA TGGTGACTAT TACCTTAACA TTAAGGGAAG AATAATAAAG 
ATTTATTGTG CAGACATGTA CTTGGAGAAC CCTAAGGAAT ATTTAACACT GGTCCAAGGT 
GAAGAAAACT TTTCTGAAGT GTATGGCTTT AGACTAAAAA ATCCATATCA ATGTCCTTTT 
AATGGGAGTA GAAGGGAAGA CTGTGAATGT GACAATGGAC ACTTAGCTGC TGGATACACT 
GTTTTCAGCA AAATAAGAAT TGATCTCACT TCCATGCAAA TTAAAACTAC GGACCTTCTT 
TTTTCCAAAA CAATATTTGG AAATGCAGTT CCATTTGCCA CAGCTGGAGA TTGCTACAGT 
GCTTTCAGAT GCCCACAGGG ACAGTTTAGC ATTAATTTGT CAGGAACTGG GATGAAGATA 
TCCAGCACAG CAAAGTGGCT CACTCAGGGG AGTTATACCT CTGTCAGCAT ACGAAGATCA 
GAGGATGGAA CTAGATTTTT CGGCAAATGT GGAGGGTACT GTGGAAAGTG TCTTCCTCAC 
ATGACTACTG GTCTCCCAAT TCAAGTCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_011538754.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537056.1 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

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CloneID	OHu72529
Clone ID Related Accession (Same CDS sequence)	XM_011538754.2
Accession Version	XM_011538754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAG AGAATATATG CATGCATCTG TGGTGCACAA GCACAGAAAA GCTTCACAAA 
GGCTGTTTCA CTCAACACGT GCCACCAGCA GATGGAACAG ACTGCGGTCC TGGAATGCAT 
TGCCGTCATG GGCTATGTGT AAACAAAGAA ACGGAAACAC GTCCTGTAAA TGGTGAATGG 
GGACCATGGG AACCTTACAG TTCTTGTTCA AGAACATGTG GAGGCGGAAT CGAAAGTGCA 
ACCAGGCGCT GTAATCGTCC TGAGCCAAGA AACGGAGGAA ATTACTGTGT GGGCCGCAGG 
ATGAAATTTC GATCATGTAA TACTGATTCA TGTCCAAAAG GCACACAAGA CTTTCGAGAG 
AAGCAGTGCT CTGATTTTAA TGGTAAACAT TTGGACATCA GTGGCATTCC CTCTAATGTG 
AGGTGGCTTC CAAGATACAG TGGCATTGGC ACAAAGGATC GTTGTAAACT CTATTGTCAG 
GTTGCTGGAA CCAATTATTT CTACCTATTG AAGGATATGG TTGAAGATGG TACTCCTTGT 
GGAACTGAAA CTCATGACAT CTGTGTTCAA GGCCAGTGTA TGGCAGCTGG TTGTGATCAC 
GTGTTAAACT CCAGTGCCAA GATAGACAAA TGTGGAGTGT GTGGTGGGGA CAACTCTTCA 
TGCAAGACAA TAACAGGTGT CTTCAACAGT TCTCATTATG GTTATAATGT TGTTGTAAAG 
ATTCCCGCAG GAGCAACAAA CGTTGACATT CGTCAGTACA GCTATTCTGG ACAACCAGAT 
GACAGTTACC TTGCATTATC TGACGCTGAA GGGAATTTTC TTTTCAATGG AAATTTTCTT 
CTAAGTACGT CAAAAAAAGA AATCAATGTG CAAGGAACAA GAACTGTTAT TGAATACAGT 
GGATCAAATA ACGCAGTTGA AAGAATTAAT AGTACTAATC GACAAGAGAA AGAACTTATT 
TTGCAGGTGT TGTGTGTGGG TAATTTATAC AACCCTGATG TACATTATTC CTTCAATATC 
CCTTTGGAAG AGAGGAGTGA CATGTTCACA TGGGACCCCT ATGGACCATG GGAAGGCTGT 
ACCAAAATGT GTCAAGGTCT TCAGCGAAGA AACATAACTT GCATACATAA GAGTGATCAT 
AGTGTTGTGT CTGATAAAGA ATGTGACCAC TTGCCACTTC CATCATTTGT TACTCAAAGT 
TGCAATACAG ACTGTGAACT AAGGTGGCAT GTTATTGGCA AAAGTGAATG TTCATCCCAA 
TGTGGTCAAG GATATAGAAC CTTGGACATC CATTGCATGA AGTATTCCAT TCATGAAGGA 
CAGACTGTTC AAGTTGATGA CCACTACTGT GGTGACCAGC TTAAACCTCC TACCCAAGAA 
CTATGCCATG GTAACTGTGT CTTCACAAGA TGGCATTATT CAGAATGGTC TCAGTGTTCC 
AGGAGTTGTG GAGGAGGGGA AAGGTCTCGA GAATCTTATT GTATGAATAA CTTTGGCCAT 
CGTCTTGCTG ACAATGAATG CCAAGAACTG TCCCGAGTGA CGAGAGAGAA TTGCAATGAA 
TTTTCCTGTC CCAGTTGGGC TGCTAGTGAA TGGAGCGAGT GCCTTGTTAC ATGTGGTAAA 
GGAACAAAGC AGCGGCAGGT ATGGTGTCAG CTGAATGTAG ATCACTTGAG TGATGGCTTC 
TGTAATTCAA GTACCAAACC TGAATCTCTG AGTCCATGTG AACTTCATAC ATGTGCTTCC 
TGGCAAGTAG GACCATGGGG TCCTTGCACA ACCACATGTG GACATGGGTA TCAGATGCGA 
GATGTTAAAT GTGTCAATGA GCTAGCTAGT GCAGTGTTAG AGGACACAGA ATGCCATGAA 
GCTAGTCGCC CCAGTGACAG ACAGAGCTGT GTACTTACAC CTTGCTCATT TATTTCTAAA 
CTTGAGACCG CTTTATTACC AACTGTTCTC ATAAAAAAGA TGGCACAATG GCGACATGGT 
TCTTGGACCC CATGCTCCGT ATCTTGTGGA AGAGGTACTC AAGCCCGCTA TGTAAGCTGT 
CGTGATGCTC TTGATAGAAT AGCAGATGAA TCATATTGTG CCCACTTACC CCGACCTGCT 
GAAATATGGG ACTGTTTTAC CCCTTGTGGA GAGTGGCAAG CAGGGGATTG GTCACCCTGT 
TCAGCTTCCT GTGGCCATGG AAAAACAACT CGACAAGTTT TATGCATGAA CTACCATCAG 
CCAATTGATG AGAATTACTG TGATCCTGAA GTTCGCCCTT TGATGGAACA GGAATGTAGC 
CTGGCAGCCT GCCCTCCTGC ACACAGCCAC TTTCCTAGTT CCCCTGTGCA GCCAAGCTAT 
TATCTAAGCA CGAATTTGCC ATTAACTCAA AAACTTGAAG ATAATGAAAA TCAGGTGGTC 
CATCCATCAG TCAGAGGAAA CCAGTGGAGA ACCGGACCAT GGGGATCATG CTCCAGCAGT 
TGTTCTGGAG GTCTTCAGCA TAGGGCTGTG GTCTGCCAGG ATGAAAATGG ACAAAGTGCT 
AGTTACTGCG ATGCAGCCTC CAAGCCTCCA GAGTTACAGC AATGTGGTCC AGGGCCTTGT 
CCACAGTGGA ACTACGGAAA TTGGGGAGAA TGTTCACAAA CATGTGGAGG AGGAATAAAA 
TCAAGACTTG TAATATGTCA ATTTCCCAAT GGCCAAATAT TAGAAGATCA CAACTGTGAA 
ATTGTAAACA AGCCACCTAG CGTAATACAG TGTCATATGC ATGCTTGCCC TGCTGATGTG 
TCATGGCATC AGGAACCATG GACATCGTGC TCAGCTTCTT GTGGTAAAGG TAGAAAGTAC 
CGTGAAGTGT TTTGCATTGA CCAATTCCAA AGGAAATTAG AAGACACAAA TTGCAGTCAA 
GTACAGAAAC CTCCAACTCA CAAAGCCTGT AGATCTGTCA GATGCCCTTC ATGGAAAGCC 
AATAGCTGGA ATGAGTGCTC TGTGACCTGT GGCTCTGGAG TTCAGCAGAG GGATGTATAC 
TGCAGACTGA AAGGTGTTGG TCAGGTGGTT GAAGAAATGT GTGATCAGTC CACCCGACCT 
TGTTCTCAGA GGCGATGTTG GAGTCAGGAC TGTGTGCAGC ACAAGGGGAT GGAAAGAGGG 
AGGCTGAATT GTTCAACATC ATGTGAGAGA AAAGATTCAC ATCAACGAAT GGAGTGCACA 
GATAACCAAA TCAGACAAGT GAATGAAATA GTCTATAATT CTTCAACCAT ATCTCTTACA 
TCCAAGAATT GCAGGAACCC TCCTTGCAAT TACATTGTGG TAACAGCAGA CTCATCACAG 
TGTGCAAACA ACTGTGGATT TAGTTACAGA CAAAGGATTA CATATTGCAC CGAGATCCCA 
TCTACTAAGA AACATAAGCT CCATCGACTT CGGCCTATAG TTTATCAAGA ATGCCCTGTG 
GTGCCTTCCT CTCAGGTTTA CCAATGCATT AACAGCTGTT TGCATTTGGC CACTTGGAAA 
GTTGGAAAAT GGAGCAAGTG CTCAGTGACT TGTGGAATTG GGATAATGAA GAGACAAGTG 
AAATGCATTA CCAAACATGG TTTGTCCAGT GACTTATGTT TAAACCATTT AAAACCAGGT 
GCTCAAAAGA AATGTTATGC CAATGACTGT AAATCATTTA CCACCTGCAA AGAAATTCAA 
GTGAAAAACC ACATTAGAAA GGATGGTGAC TATTACCTTA ACATTAAGGG AAGAATAATA 
AAGATTTATT GTGCAGACAT GTACTTGGAG AACCCTAAGG AATATTTAAC ACTGGTCCAA 
GGTGAAGAAA ACTTTTCTGA AGTGTATGGC TTTAGACTAA AAAATCCATA TCAATGTCCT 
TTTAATGGGA GTAGAAGGGA AGACTGTGAA TGTGACAATG GACACTTAGC TGCTGGATAC 
ACTGTTTTCA GCAAAATAAG AATTGATCTC ACTTCCATGC AAATTAAAAC TACGGACCTT 
CTTTTTTCCA AAACAATATT TGGAAATGCA GTTCCATTTG CCACAGCTGG AGATTGCTAC 
AGTGCTTTCA GATGCCCACA GGGACAGTTT AGCATTAATT TGTCAGGAAC TGGGATGAAG 
ATATCCAGCA CAGCAAAGTG GCTCACTCAG GGGAGTTATA CCTCTGTCAG CATACGAAGA 
TCAGAGGATG GAACTAGATT TTTCGGCAAA TGTGGAGGGT ACTGTGGAAA GTGTCTTCCT 
CACATGACTA CTGGTCTCCC AATTCAAGTC ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537056.1
CDS	51..5786
Translation	MWVAKWLTGLLYHLSLFITRSWEVDFHPRQEALVRTLTSYEVVIPERVNEFGEVFPQSHHFSRQKRSSEALEPMPFRTHYRFTAYGQLFQLNLTADASFLAAGYTEVHLGTPERGAWESDAGPSDLRHCFYRGQVNSQEDYKAVVSLCGGLTGTFKGQNGEYFLEPIMKADGNEYEDGHNKPHLIYRQDLNNSFLQTLKYCSVSESQIKETSLPFHTYSNMNEDLNVMKERVLGHTSKNVPLKDERRHSRKKRLISYPRYIEIMVTADAKVVSAHGSNLQNYILTLMSIVATIYKDPSIGNLIHIVVVKLVMIHREEEGPVINFDGATTLKNFCSWQQTQNDLDDVHPSHHDTAVLITREDICSSKEKCNMLGLSYLGTICDPLQSCFINEEKGLISAFTIAHELGHTLGVQHDDNPRCKEMKVTKYHVMAPALSFHMSPWSWSNCSRKYVTEFLDTGYGECLLDKPDEEIYNLPSELPGSRYDGNKQCELAFGPGSQMCPHIENICMHLWCTSTEKLHKGCFTQHVPPADGTDCGPGMHCRHGLCVNKETETRPVNGEWGPWEPYSSCSRTCGGGIESATRRCNRPEPRNGGNYCVGRRMKFRSCNTDSCPKGTQDFREKQCSDFNGKHLDISGIPSNVRWLPRYSGIGTKDRCKLYCQVAGTNYFYLLKDMVEDGTPCGTETHDICVQGQCMAAGCDHVLNSSAKIDKCGVCGGDNSSCKTITGVFNSSHYGYNVVVKIPAGATNVDIRQYSYSGQPDDSYLALSDAEGNFLFNGNFLLSTSKKEINVQGTRTVIEYSGSNNAVERINSTNRQEKELILQVLCVGNLYNPDVHYSFNIPLEERSDMFTWDPYGPWEGCTKMCQGLQRRNITCIHKSDHSVVSDKECDHLPLPSFVTQSCNTDCELRWHVIGKSECSSQCGQGYRTLDIHCMKYSIHEGQTVQVDDHYCGDQLKPPTQELCHGNCVFTRWHYSEWSQCSRSCGGGERSRESYCMNNFGHRLADNECQELSRVTRENCNEFSCPSWAASEWSECLVTCGKGTKQRQVWCQLNVDHLSDGFCNSSTKPESLSPCELHTCASWQVGPWGPCTTTCGHGYQMRDVKCVNELASAVLEDTECHEASRPSDRQSCVLTPCSFISKLETALLPTVLIKKMAQWRHGSWTPCSVSCGRGTQARYVSCRDALDRIADESYCAHLPRPAEIWDCFTPCGEWQAGDWSPCSASCGHGKTTRQVLCMNYHQPIDENYCDPEVRPLMEQECSLAACPPAHSHFPSSPVQPSYYLSTNLPLTQKLEDNENQVVHPSVRGNQWRTGPWGSCSSSCSGGLQHRAVVCQDENGQSASYCDAASKPPELQQCGPGPCPQWNYGNWGECSQTCGGGIKSRLVICQFPNGQILEDHNCEIVNKPPSVIQCHMHACPADVSWHQEPWTSCSASCGKGRKYREVFCIDQFQRKLEDTNCSQVQKPPTHKACRSVRCPSWKANSWNECSVTCGSGVQQRDVYCRLKGVGQVVEEMCDQSTRPCSQRRCWSQDCVQHKGMERGRLNCSTSCERKDSHQRMECTDNQIRQVNEIVYNSSTISLTSKNCRNPPCNYIVVTADSSQCANNCGFSYRQRITYCTEIPSTKKHKLHRLRPIVYQECPVVPSSQVYQCINSCLHLATWKVGKWSKCSVTCGIGIMKRQVKCITKHGLSSDLCLNHLKPGAQKKCYANDCKSFTTCKEIQVKNHIRKDGDYYLNIKGRIIKIYCADMYLENPKEYLTLVQGEENFSEVYGFRLKNPYQCPFNGSRREDCECDNGHLAAGYTVFSKIRIDLTSMQIKTTDLLFSKTIFGNAVPFATAGDCYSAFRCPQGQFSINLSGTGMKISSTAKWLTQGSYTSVSIRRSEDGTRFFGKCGGYCGKCLPHMTTGLPIQVI

Target ORF information:

RefSeq Version	XM_011538754.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538754.2

ORF Insert Sequence:

ATGTGGGTGG CCAAGTGGCT GACTGGGCTG CTCTACCATC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTGACTTCCA CCCCAGGCAA GAAGCCCTGG TGAGGACACT GACCTCCTAC 
GAAGTAGTGA TCCCCGAGCG GGTCAATGAG TTTGGAGAAG TGTTCCCTCA GAGCCACCAC 
TTCAGCCGGC AGAAACGCAG CTCCGAGGCG CTGGAACCCA TGCCGTTCCG AACCCACTAT 
CGCTTCACTG CCTACGGGCA GCTCTTCCAG CTGAACCTGA CCGCCGATGC ATCCTTTCTG 
GCCGCCGGCT ACACCGAGGT GCACTTGGGA ACCCCGGAGC GCGGGGCCTG GGAGAGCGAC 
GCAGGGCCCT CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACTC ACAGGAGGAT 
TACAAGGCCG TCGTCAGCTT ATGCGGAGGC CTGACGGGAA CATTTAAAGG ACAGAACGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAATG AATATGAAGA TGGTCACAAC 
AAGCCACATC TTATATACAG ACAAGACTTA AATAACTCTT TTCTGCAGAC TCTGAAGTAT 
TGCAGTGTGT CAGAAAGTCA AATAAAGGAA ACCAGTTTAC CCTTTCATAC CTACAGCAAC 
ATGAATGAAG ATCTTAATGT AATGAAAGAA AGAGTTTTAG GACACACATC AAAAAATGTA 
CCATTGAAAG ATGAAAGAAG ACATTCCAGG AAAAAACGTC TTATATCATA TCCAAGATAC 
ATTGAAATTA TGGTTACAGC TGATGCTAAA GTGGTTTCTG CTCATGGATC GAATTTGCAA 
AACTATATAC TGACTCTAAT GTCAATTGTT GCAACAATCT ACAAAGATCC AAGTATTGGA 
AATTTGATAC ACATAGTAGT GGTAAAATTA GTTATGATTC ACCGTGAGGA GGAAGGACCA 
GTCATTAATT TTGATGGTGC TACCACATTA AAGAACTTTT GTTCATGGCA ACAAACTCAG 
AATGACCTTG ATGATGTTCA CCCTTCCCAC CATGACACTG CTGTTCTTAT CACTAGGGAA 
GACATTTGTT CATCTAAAGA GAAATGTAAC ATGTTAGGTT TATCATATTT AGGTACCATA 
TGTGATCCTT TACAAAGCTG CTTTATTAAT GAAGAAAAAG GACTCATTTC TGCTTTTACT 
ATAGCCCATG AGCTTGGGCA CACACTTGGT GTTCAACATG ATGATAATCC TAGATGTAAA 
GAAATGAAAG TTACAAAGTA TCATGTAATG GCCCCTGCTT TAAGTTTTCA CATGAGTCCT 
TGGAGCTGGT CAAACTGTAG TCGGAAATAT GTTACTGAAT TCCTAGATAC TGGTTACGGG 
GAATGTCTTC TTGACAAACC AGATGAAGAA ATATATAATC TGCCTTCAGA ACTTCCTGGA 
TCACGATATG ATGGAAACAA GCAGTGTGAG CTTGCGTTTG GTCCTGGGTC ACAAATGTGT 
CCCCATATAG AGAATATATG CATGCATCTG TGGTGCACAA GCACAGAAAA GCTTCACAAA 
GGCTGTTTCA CTCAACACGT GCCACCAGCA GATGGAACAG ACTGCGGTCC TGGAATGCAT 
TGCCGTCATG GGCTATGTGT AAACAAAGAA ACGGAAACAC GTCCTGTAAA TGGTGAATGG 
GGACCATGGG AACCTTACAG TTCTTGTTCA AGAACATGTG GAGGCGGAAT CGAAAGTGCA 
ACCAGGCGCT GTAATCGTCC TGAGCCAAGA AACGGAGGAA ATTACTGTGT GGGCCGCAGG 
ATGAAATTTC GATCATGTAA TACTGATTCA TGTCCAAAAG GCACACAAGA CTTTCGAGAG 
AAGCAGTGCT CTGATTTTAA TGGTAAACAT TTGGACATCA GTGGCATTCC CTCTAATGTG 
AGGTGGCTTC CAAGATACAG TGGCATTGGC ACAAAGGATC GTTGTAAACT CTATTGTCAG 
GTTGCTGGAA CCAATTATTT CTACCTATTG AAGGATATGG TTGAAGATGG TACTCCTTGT 
GGAACTGAAA CTCATGACAT CTGTGTTCAA GGCCAGTGTA TGGCAGCTGG TTGTGATCAC 
GTGTTAAACT CCAGTGCCAA GATAGACAAA TGTGGAGTGT GTGGTGGGGA CAACTCTTCA 
TGCAAGACAA TAACAGGTGT CTTCAACAGT TCTCATTATG GTTATAATGT TGTTGTAAAG 
ATTCCCGCAG GAGCAACAAA CGTTGACATT CGTCAGTACA GCTATTCTGG ACAACCAGAT 
GACAGTTACC TTGCATTATC TGACGCTGAA GGGAATTTTC TTTTCAATGG AAATTTTCTT 
CTAAGTACGT CAAAAAAAGA AATCAATGTG CAAGGAACAA GAACTGTTAT TGAATACAGT 
GGATCAAATA ACGCAGTTGA AAGAATTAAT AGTACTAATC GACAAGAGAA AGAACTTATT 
TTGCAGGTGT TGTGTGTGGG TAATTTATAC AACCCTGATG TACATTATTC CTTCAATATC 
CCTTTGGAAG AGAGGAGTGA CATGTTCACA TGGGACCCCT ATGGACCATG GGAAGGCTGT 
ACCAAAATGT GTCAAGGTCT TCAGCGAAGA AACATAACTT GCATACATAA GAGTGATCAT 
AGTGTTGTGT CTGATAAAGA ATGTGACCAC TTGCCACTTC CATCATTTGT TACTCAAAGT 
TGCAATACAG ACTGTGAACT AAGGTGGCAT GTTATTGGCA AAAGTGAATG TTCATCCCAA 
TGTGGTCAAG GATATAGAAC CTTGGACATC CATTGCATGA AGTATTCCAT TCATGAAGGA 
CAGACTGTTC AAGTTGATGA CCACTACTGT GGTGACCAGC TTAAACCTCC TACCCAAGAA 
CTATGCCATG GTAACTGTGT CTTCACAAGA TGGCATTATT CAGAATGGTC TCAGTGTTCC 
AGGAGTTGTG GAGGAGGGGA AAGGTCTCGA GAATCTTATT GTATGAATAA CTTTGGCCAT 
CGTCTTGCTG ACAATGAATG CCAAGAACTG TCCCGAGTGA CGAGAGAGAA TTGCAATGAA 
TTTTCCTGTC CCAGTTGGGC TGCTAGTGAA TGGAGCGAGT GCCTTGTTAC ATGTGGTAAA 
GGAACAAAGC AGCGGCAGGT ATGGTGTCAG CTGAATGTAG ATCACTTGAG TGATGGCTTC 
TGTAATTCAA GTACCAAACC TGAATCTCTG AGTCCATGTG AACTTCATAC ATGTGCTTCC 
TGGCAAGTAG GACCATGGGG TCCTTGCACA ACCACATGTG GACATGGGTA TCAGATGCGA 
GATGTTAAAT GTGTCAATGA GCTAGCTAGT GCAGTGTTAG AGGACACAGA ATGCCATGAA 
GCTAGTCGCC CCAGTGACAG ACAGAGCTGT GTACTTACAC CTTGCTCATT TATTTCTAAA 
CTTGAGACCG CTTTATTACC AACTGTTCTC ATAAAAAAGA TGGCACAATG GCGACATGGT 
TCTTGGACCC CATGCTCCGT ATCTTGTGGA AGAGGTACTC AAGCCCGCTA TGTAAGCTGT 
CGTGATGCTC TTGATAGAAT AGCAGATGAA TCATATTGTG CCCACTTACC CCGACCTGCT 
GAAATATGGG ACTGTTTTAC CCCTTGTGGA GAGTGGCAAG CAGGGGATTG GTCACCCTGT 
TCAGCTTCCT GTGGCCATGG AAAAACAACT CGACAAGTTT TATGCATGAA CTACCATCAG 
CCAATTGATG AGAATTACTG TGATCCTGAA GTTCGCCCTT TGATGGAACA GGAATGTAGC 
CTGGCAGCCT GCCCTCCTGC ACACAGCCAC TTTCCTAGTT CCCCTGTGCA GCCAAGCTAT 
TATCTAAGCA CGAATTTGCC ATTAACTCAA AAACTTGAAG ATAATGAAAA TCAGGTGGTC 
CATCCATCAG TCAGAGGAAA CCAGTGGAGA ACCGGACCAT GGGGATCATG CTCCAGCAGT 
TGTTCTGGAG GTCTTCAGCA TAGGGCTGTG GTCTGCCAGG ATGAAAATGG ACAAAGTGCT 
AGTTACTGCG ATGCAGCCTC CAAGCCTCCA GAGTTACAGC AATGTGGTCC AGGGCCTTGT 
CCACAGTGGA ACTACGGAAA TTGGGGAGAA TGTTCACAAA CATGTGGAGG AGGAATAAAA 
TCAAGACTTG TAATATGTCA ATTTCCCAAT GGCCAAATAT TAGAAGATCA CAACTGTGAA 
ATTGTAAACA AGCCACCTAG CGTAATACAG TGTCATATGC ATGCTTGCCC TGCTGATGTG 
TCATGGCATC AGGAACCATG GACATCGTGC TCAGCTTCTT GTGGTAAAGG TAGAAAGTAC 
CGTGAAGTGT TTTGCATTGA CCAATTCCAA AGGAAATTAG AAGACACAAA TTGCAGTCAA 
GTACAGAAAC CTCCAACTCA CAAAGCCTGT AGATCTGTCA GATGCCCTTC ATGGAAAGCC 
AATAGCTGGA ATGAGTGCTC TGTGACCTGT GGCTCTGGAG TTCAGCAGAG GGATGTATAC 
TGCAGACTGA AAGGTGTTGG TCAGGTGGTT GAAGAAATGT GTGATCAGTC CACCCGACCT 
TGTTCTCAGA GGCGATGTTG GAGTCAGGAC TGTGTGCAGC ACAAGGGGAT GGAAAGAGGG 
AGGCTGAATT GTTCAACATC ATGTGAGAGA AAAGATTCAC ATCAACGAAT GGAGTGCACA 
GATAACCAAA TCAGACAAGT GAATGAAATA GTCTATAATT CTTCAACCAT ATCTCTTACA 
TCCAAGAATT GCAGGAACCC TCCTTGCAAT TACATTGTGG TAACAGCAGA CTCATCACAG 
TGTGCAAACA ACTGTGGATT TAGTTACAGA CAAAGGATTA CATATTGCAC CGAGATCCCA 
TCTACTAAGA AACATAAGCT CCATCGACTT CGGCCTATAG TTTATCAAGA ATGCCCTGTG 
GTGCCTTCCT CTCAGGTTTA CCAATGCATT AACAGCTGTT TGCATTTGGC CACTTGGAAA 
GTTGGAAAAT GGAGCAAGTG CTCAGTGACT TGTGGAATTG GGATAATGAA GAGACAAGTG 
AAATGCATTA CCAAACATGG TTTGTCCAGT GACTTATGTT TAAACCATTT AAAACCAGGT 
GCTCAAAAGA AATGTTATGC CAATGACTGT AAATCATTTA CCACCTGCAA AGAAATTCAA 
GTGAAAAACC ACATTAGAAA GGATGGTGAC TATTACCTTA ACATTAAGGG AAGAATAATA 
AAGATTTATT GTGCAGACAT GTACTTGGAG AACCCTAAGG AATATTTAAC ACTGGTCCAA 
GGTGAAGAAA ACTTTTCTGA AGTGTATGGC TTTAGACTAA AAAATCCATA TCAATGTCCT 
TTTAATGGGA GTAGAAGGGA AGACTGTGAA TGTGACAATG GACACTTAGC TGCTGGATAC 
ACTGTTTTCA GCAAAATAAG AATTGATCTC ACTTCCATGC AAATTAAAAC TACGGACCTT 
CTTTTTTCCA AAACAATATT TGGAAATGCA GTTCCATTTG CCACAGCTGG AGATTGCTAC 
AGTGCTTTCA GATGCCCACA GGGACAGTTT AGCATTAATT TGTCAGGAAC TGGGATGAAG 
ATATCCAGCA CAGCAAAGTG GCTCACTCAG GGGAGTTATA CCTCTGTCAG CATACGAAGA 
TCAGAGGATG GAACTAGATT TTTCGGCAAA TGTGGAGGGT ACTGTGGAAA GTGTCTTCCT 
CACATGACTA CTGGTCTCCC AATTCAAGTC ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_017019979.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875468.1 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

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CloneID	OHu72530
Clone ID Related Accession (Same CDS sequence)	XM_017019979.1
Accession Version	XM_017019979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAC TTGGTGTTCA ACATGATGAT AATCCTAGAT GTAAAGAAAT GAAAGTTACA 
AAGTATCATG TAATGGCCCC TGCTTTAAGT TTTCACATGA GTCCTTGGAG CTGGTCAAAC 
TGTAGTCGGA AATATGTTAC TGAATTCCTA GATACTGGTT ACGGGGAATG TCTTCTTGAC 
AAACCAGATG AAGAAATATA TAATCTGCCT TCAGAACTTC CTGGATCACG ATATGATGGA 
AACAAGCAGT GTGAGCTTGC GTTTGGTCCT GGGTCACAAA TGTGTCCCCA TATAGAGAAT 
ATATGCATGC ATCTGTGGTG CACAAGCACA GAAAAGCTTC ACAAAGGCTG TTTCACTCAA 
CACGTGCCAC CAGCAGATGG AACAGACTGC GGTCCTGGAA TGCATTGCCG TCATGGGCTA 
TGTGTAAACA AAGAAACGGA AACACGTCCT GTAAATGGTG AATGGGGACC ATGGGAACCT 
TACAGTTCTT GTTCAAGAAC ATGTGGAGGC GGAATCGAAA GTGCAACCAG GCGCTGTAAT 
CGTCCTGAGC CAAGAAACGG AGGAAATTAC TGTGTGGGCC GCAGGATGAA ATTTCGATCA 
TGTAATACTG ATTCATGTCC AAAAGGCACA CAAGACTTTC GAGAGAAGCA GTGCTCTGAT 
TTTAATGGTA AACATTTGGA CATCAGTGGC ATTCCCTCTA ATGTGAGGTG GCTTCCAAGA 
TACAGTGGCA TTGGCACAAA GGATCGTTGT AAACTCTATT GTCAGGTTGC TGGAACCAAT 
TATTTCTACC TATTGAAGGA TATGGTTGAA GATGGTACTC CTTGTGGAAC TGAAACTCAT 
GACATCTGTG TTCAAGGCCA GTGTATGGCA GCTGGTTGTG ATCACGTGTT AAACTCCAGT 
GCCAAGATAG ACAAATGTGG AGTGTGTGGT GGGGACAACT CTTCATGCAA GACAATAACA 
GGTGTCTTCA ACAGTTCTCA TTATGGTTAT AATGTTGTTG TAAAGATTCC CGCAGGAGCA 
ACAAACGTTG ACATTCGTCA GTACAGCTAT TCTGGACAAC CAGATGACAG TTACCTTGCA 
TTATCTGACG CTGAAGGGAA TTTTCTTTTC AATGGAAATT TTCTTCTAAG TACGTCAAAA 
AAAGAAATCA ATGTGCAAGG AACAAGAACT GTTATTGAAT ACAGTGGATC AAATAACGCA 
GTTGAAAGAA TTAATAGTAC TAATCGACAA GAGAAAGAAC TTATTTTGCA GGTGTTGTGT 
GTGGGTAATT TATACAACCC TGATGTACAT TATTCCTTCA ATATCCCTTT GGAAGAGAGG 
AGTGACATGT TCACATGGGA CCCCTATGGA CCATGGGAAG GCTGTACCAA AATGTGTCAA 
GGTCTTCAGC GAAGAAACAT AACTTGCATA CATAAGAGTG ATCATAGTGT TGTGTCTGAT 
AAAGAATGTG ACCACTTGCC ACTTCCATCA TTTGTTACTC AAAGTTGCAA TACAGACTGT 
GAACTAAGGT GGCATGTTAT TGGCAAAAGT GAATGTTCAT CCCAATGTGG TCAAGGATAT 
AGAACCTTGG ACATCCATTG CATGAAGTAT TCCATTCATG AAGGACAGAC TGTTCAAGTT 
GATGACCACT ACTGTGGTGA CCAGCTTAAA CCTCCTACCC AAGAACTATG CCATGGTAAC 
TGTGTCTTCA CAAGATGGCA TTATTCAGAA TGGTCTCAGT GTTCCAGGAG TTGTGGAGGA 
GGGGAAAGGT CTCGAGAATC TTATTGTATG AATAACTTTG GCCATCGTCT TGCTGACAAT 
GAATGCCAAG AACTGTCCCG AGTGACGAGA GAGAATTGCA ATGAATTTTC CTGTCCCAGT 
TGGGCTGCTA GTGAATGGAG CGAGTGCCTT GTTACATGTG GTAAAGGAAC AAAGCAGCGG 
CAGGTATGGT GTCAGCTGAA TGTAGATCAC TTGAGTGATG GCTTCTGTAA TTCAAGTACC 
AAACCTGAAT CTCTGAGTCC ATGTGAACTT CATACATGTG CTTCCTGGCA AGTAGGACCA 
TGGGGTCCTT GCACAACCAC ATGTGGACAT GGGTATCAGA TGCGAGATGT TAAATGTGTC 
AATGAGCTAG CTAGTGCAGT GTTAGAGGAC ACAGAATGCC ATGAAGCTAG TCGCCCCAGT 
GACAGACAGA GCTGTGTACT TACACCTTGC TCATTTATTT CTAAACTTGA GACCGCTTTA 
TTACCAACTG TTCTCATAAA AAAGATGGCA CAATGGCGAC ATGGTTCTTG GACCCCATGC 
TCCGTATCTT GTGGAAGAGG TACTCAAGCC CGCTATGTAA GCTGTCGTGA TGCTCTTGAT 
AGAATAGCAG ATGAATCATA TTGTGCCCAC TTACCCCGAC CTGCTGAAAT ATGGGACTGT 
TTTACCCCTT GTGGAGAGTG GCAAGCAGGG GATTGGTCAC CCTGTTCAGC TTCCTGTGGC 
CATGGAAAAA CAACTCGACA AGTTTTATGC ATGAACTACC ATCAGCCAAT TGATGAGAAT 
TACTGTGATC CTGAAGTTCG CCCTTTGATG GAACAGGAAT GTAGCCTGGC AGCCTGCCCT 
CCTGCACACA GCCACTTTCC TAGTTCCCCT GTGCAGCCAA GCTATTATCT AAGCACGAAT 
TTGCCATTAA CTCAAAAACT TGAAGATAAT GAAAATCAGG TGGTCCATCC ATCAGTCAGA 
GGAAACCAGT GGAGAACCGG ACCATGGGGA TCATGCTCCA GCAGTTGTTC TGGAGGTCTT 
CAGCATAGGG CTGTGGTCTG CCAGGATGAA AATGGACAAA GTGCTAGTTA CTGCGATGCA 
GCCTCCAAGC CTCCAGAGTT ACAGCAATGT GGTCCAGGGC CTTGTCCACA GTGGAACTAC 
GGAAATTGGG GAGAATGTTC ACAAACATGT GGAGGAGGAA TAAAATCAAG ACTTGTAATA 
TGTCAATTTC CCAATGGCCA AATATTAGAA GATCACAACT GTGAAATTGT AAACAAGCCA 
CCTAGCGTAA TACAGTGTCA TATGCATGCT TGCCCTGCTG ATGTGTCATG GCATCAGGAA 
CCATGGACAT CGTGCTCAGC TTCTTGTGGT AAAGGTAGAA AGTACCGTGA AGTGTTTTGC 
ATTGACCAAT TCCAAAGGAA ATTAGAAGAC ACAAATTGCA GTCAAGTACA GAAACCTCCA 
ACTCACAAAG CCTGTAGATC TGTCAGATGC CCTTCATGGA AAGCCAATAG CTGGAATGAG 
TGCTCTGTGA CCTGTGGCTC TGGAGTTCAG CAGAGGGATG TATACTGCAG ACTGAAAGGT 
GTTGGTCAGG TGGTTGAAGA AATGTGTGAT CAGTCCACCC GACCTTGTTC TCAGAGGCGA 
TGTTGGAGTC AGGACTGTGT GCAGCACAAG GGGATGGAAA GAGGGAGGCT GAATTGTTCA 
ACATCATGTG AGAGAAAAGA TTCACATCAA CGAATGGAGT GCACAGATAA CCAAATCAGA 
CAAGTGAATG AAATAGTCTA TAATTCTTCA ACCATATCTC TTACATCCAA GAATTGCAGG 
AACCCTCCTT GCAATTACAT TGTGGTAACA GCAGACTCAT CACAGTGTGC AAACAACTGT 
GGATTTAGTT ACAGACAAAG GATTACATAT TGCACCGAGA TCCCATCTAC TAAGAAACAT 
AAGCTCCATC GACTTCGGCC TATAGTTTAT CAAGAATGCC CTGTGGTGCC TTCCTCTCAG 
GTTTACCAAT GCATTAACAG CTGTTTGCAT TTGGCCACTT GGAAAGTTGG AAAATGGAGC 
AAGTGCTCAG TGACTTGTGG AATTGGGATA ATGAAGAGAC AAGTGAAATG CATTACCAAA 
CATGGTTTGT CCAGTGACTT ATGTTTAAAC CATTTAAAAC CAGGTGCTCA AAAGAAATGT 
TATGCCAATG ACTGTAAATC ATTTACCACC TGCAAAGAAA TTCAAGTGAA AAACCACATT 
AGAAAGGATG GTGACTATTA CCTTAACATT AAGGGAAGAA TAATAAAGAT TTATTGTGCA 
GACATGTACT TGGAGAACCC TAAGGAATAT TTAACACTGG TCCAAGGTGA AGAAAACTTT 
TCTGAAGTGT ATGGCTTTAG ACTAAAAAAT CCATATCAAT GTCCTTTTAA TGGGAGTAGA 
AGGGAAGACT GTGAATGTGA CAATGGACAC TTAGCTGCTG GATACACTGT TTTCAGCAAA 
ATAAGAATTG ATCTCACTTC CATGCAAATT AAAACTACGG ACCTTCTTTT TTCCAAAACA 
ATATTTGGAA ATGCAGTTCC ATTTGCCACA GCTGGAGATT GCTACAGTGC TTTCAGATGC 
CCACAGGGAC AGTTTAGCAT TAATTTGTCA GGAACTGGGA TGAAGATATC CAGCACAGCA 
AAGTGGCTCA CTCAGGGGAG TTATACCTCT GTCAGCATAC GAAGATCAGA GGATGGAACT 
AGATTTTTCG GCAAATGTGG AGGGTACTGT GGAAAGTGTC TTCCTCACAT GACTACTGGT 
CTCCCAATTC AAGTCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875468.1
CDS	282..4802
Translation	MTRLGVQHDDNPRCKEMKVTKYHVMAPALSFHMSPWSWSNCSRKYVTEFLDTGYGECLLDKPDEEIYNLPSELPGSRYDGNKQCELAFGPGSQMCPHIENICMHLWCTSTEKLHKGCFTQHVPPADGTDCGPGMHCRHGLCVNKETETRPVNGEWGPWEPYSSCSRTCGGGIESATRRCNRPEPRNGGNYCVGRRMKFRSCNTDSCPKGTQDFREKQCSDFNGKHLDISGIPSNVRWLPRYSGIGTKDRCKLYCQVAGTNYFYLLKDMVEDGTPCGTETHDICVQGQCMAAGCDHVLNSSAKIDKCGVCGGDNSSCKTITGVFNSSHYGYNVVVKIPAGATNVDIRQYSYSGQPDDSYLALSDAEGNFLFNGNFLLSTSKKEINVQGTRTVIEYSGSNNAVERINSTNRQEKELILQVLCVGNLYNPDVHYSFNIPLEERSDMFTWDPYGPWEGCTKMCQGLQRRNITCIHKSDHSVVSDKECDHLPLPSFVTQSCNTDCELRWHVIGKSECSSQCGQGYRTLDIHCMKYSIHEGQTVQVDDHYCGDQLKPPTQELCHGNCVFTRWHYSEWSQCSRSCGGGERSRESYCMNNFGHRLADNECQELSRVTRENCNEFSCPSWAASEWSECLVTCGKGTKQRQVWCQLNVDHLSDGFCNSSTKPESLSPCELHTCASWQVGPWGPCTTTCGHGYQMRDVKCVNELASAVLEDTECHEASRPSDRQSCVLTPCSFISKLETALLPTVLIKKMAQWRHGSWTPCSVSCGRGTQARYVSCRDALDRIADESYCAHLPRPAEIWDCFTPCGEWQAGDWSPCSASCGHGKTTRQVLCMNYHQPIDENYCDPEVRPLMEQECSLAACPPAHSHFPSSPVQPSYYLSTNLPLTQKLEDNENQVVHPSVRGNQWRTGPWGSCSSSCSGGLQHRAVVCQDENGQSASYCDAASKPPELQQCGPGPCPQWNYGNWGECSQTCGGGIKSRLVICQFPNGQILEDHNCEIVNKPPSVIQCHMHACPADVSWHQEPWTSCSASCGKGRKYREVFCIDQFQRKLEDTNCSQVQKPPTHKACRSVRCPSWKANSWNECSVTCGSGVQQRDVYCRLKGVGQVVEEMCDQSTRPCSQRRCWSQDCVQHKGMERGRLNCSTSCERKDSHQRMECTDNQIRQVNEIVYNSSTISLTSKNCRNPPCNYIVVTADSSQCANNCGFSYRQRITYCTEIPSTKKHKLHRLRPIVYQECPVVPSSQVYQCINSCLHLATWKVGKWSKCSVTCGIGIMKRQVKCITKHGLSSDLCLNHLKPGAQKKCYANDCKSFTTCKEIQVKNHIRKDGDYYLNIKGRIIKIYCADMYLENPKEYLTLVQGEENFSEVYGFRLKNPYQCPFNGSRREDCECDNGHLAAGYTVFSKIRIDLTSMQIKTTDLLFSKTIFGNAVPFATAGDCYSAFRCPQGQFSINLSGTGMKISSTAKWLTQGSYTSVSIRRSEDGTRFFGKCGGYCGKCLPHMTTGLPIQVI

Target ORF information:

RefSeq Version	XM_017019979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019979.1

ORF Insert Sequence:

ATGACCAGAC TTGGTGTTCA ACATGATGAT AATCCTAGAT GTAAAGAAAT GAAAGTTACA 
AAGTATCATG TAATGGCCCC TGCTTTAAGT TTTCACATGA GTCCTTGGAG CTGGTCAAAC 
TGTAGTCGGA AATATGTTAC TGAATTCCTA GATACTGGTT ACGGGGAATG TCTTCTTGAC 
AAACCAGATG AAGAAATATA TAATCTGCCT TCAGAACTTC CTGGATCACG ATATGATGGA 
AACAAGCAGT GTGAGCTTGC GTTTGGTCCT GGGTCACAAA TGTGTCCCCA TATAGAGAAT 
ATATGCATGC ATCTGTGGTG CACAAGCACA GAAAAGCTTC ACAAAGGCTG TTTCACTCAA 
CACGTGCCAC CAGCAGATGG AACAGACTGC GGTCCTGGAA TGCATTGCCG TCATGGGCTA 
TGTGTAAACA AAGAAACGGA AACACGTCCT GTAAATGGTG AATGGGGACC ATGGGAACCT 
TACAGTTCTT GTTCAAGAAC ATGTGGAGGC GGAATCGAAA GTGCAACCAG GCGCTGTAAT 
CGTCCTGAGC CAAGAAACGG AGGAAATTAC TGTGTGGGCC GCAGGATGAA ATTTCGATCA 
TGTAATACTG ATTCATGTCC AAAAGGCACA CAAGACTTTC GAGAGAAGCA GTGCTCTGAT 
TTTAATGGTA AACATTTGGA CATCAGTGGC ATTCCCTCTA ATGTGAGGTG GCTTCCAAGA 
TACAGTGGCA TTGGCACAAA GGATCGTTGT AAACTCTATT GTCAGGTTGC TGGAACCAAT 
TATTTCTACC TATTGAAGGA TATGGTTGAA GATGGTACTC CTTGTGGAAC TGAAACTCAT 
GACATCTGTG TTCAAGGCCA GTGTATGGCA GCTGGTTGTG ATCACGTGTT AAACTCCAGT 
GCCAAGATAG ACAAATGTGG AGTGTGTGGT GGGGACAACT CTTCATGCAA GACAATAACA 
GGTGTCTTCA ACAGTTCTCA TTATGGTTAT AATGTTGTTG TAAAGATTCC CGCAGGAGCA 
ACAAACGTTG ACATTCGTCA GTACAGCTAT TCTGGACAAC CAGATGACAG TTACCTTGCA 
TTATCTGACG CTGAAGGGAA TTTTCTTTTC AATGGAAATT TTCTTCTAAG TACGTCAAAA 
AAAGAAATCA ATGTGCAAGG AACAAGAACT GTTATTGAAT ACAGTGGATC AAATAACGCA 
GTTGAAAGAA TTAATAGTAC TAATCGACAA GAGAAAGAAC TTATTTTGCA GGTGTTGTGT 
GTGGGTAATT TATACAACCC TGATGTACAT TATTCCTTCA ATATCCCTTT GGAAGAGAGG 
AGTGACATGT TCACATGGGA CCCCTATGGA CCATGGGAAG GCTGTACCAA AATGTGTCAA 
GGTCTTCAGC GAAGAAACAT AACTTGCATA CATAAGAGTG ATCATAGTGT TGTGTCTGAT 
AAAGAATGTG ACCACTTGCC ACTTCCATCA TTTGTTACTC AAAGTTGCAA TACAGACTGT 
GAACTAAGGT GGCATGTTAT TGGCAAAAGT GAATGTTCAT CCCAATGTGG TCAAGGATAT 
AGAACCTTGG ACATCCATTG CATGAAGTAT TCCATTCATG AAGGACAGAC TGTTCAAGTT 
GATGACCACT ACTGTGGTGA CCAGCTTAAA CCTCCTACCC AAGAACTATG CCATGGTAAC 
TGTGTCTTCA CAAGATGGCA TTATTCAGAA TGGTCTCAGT GTTCCAGGAG TTGTGGAGGA 
GGGGAAAGGT CTCGAGAATC TTATTGTATG AATAACTTTG GCCATCGTCT TGCTGACAAT 
GAATGCCAAG AACTGTCCCG AGTGACGAGA GAGAATTGCA ATGAATTTTC CTGTCCCAGT 
TGGGCTGCTA GTGAATGGAG CGAGTGCCTT GTTACATGTG GTAAAGGAAC AAAGCAGCGG 
CAGGTATGGT GTCAGCTGAA TGTAGATCAC TTGAGTGATG GCTTCTGTAA TTCAAGTACC 
AAACCTGAAT CTCTGAGTCC ATGTGAACTT CATACATGTG CTTCCTGGCA AGTAGGACCA 
TGGGGTCCTT GCACAACCAC ATGTGGACAT GGGTATCAGA TGCGAGATGT TAAATGTGTC 
AATGAGCTAG CTAGTGCAGT GTTAGAGGAC ACAGAATGCC ATGAAGCTAG TCGCCCCAGT 
GACAGACAGA GCTGTGTACT TACACCTTGC TCATTTATTT CTAAACTTGA GACCGCTTTA 
TTACCAACTG TTCTCATAAA AAAGATGGCA CAATGGCGAC ATGGTTCTTG GACCCCATGC 
TCCGTATCTT GTGGAAGAGG TACTCAAGCC CGCTATGTAA GCTGTCGTGA TGCTCTTGAT 
AGAATAGCAG ATGAATCATA TTGTGCCCAC TTACCCCGAC CTGCTGAAAT ATGGGACTGT 
TTTACCCCTT GTGGAGAGTG GCAAGCAGGG GATTGGTCAC CCTGTTCAGC TTCCTGTGGC 
CATGGAAAAA CAACTCGACA AGTTTTATGC ATGAACTACC ATCAGCCAAT TGATGAGAAT 
TACTGTGATC CTGAAGTTCG CCCTTTGATG GAACAGGAAT GTAGCCTGGC AGCCTGCCCT 
CCTGCACACA GCCACTTTCC TAGTTCCCCT GTGCAGCCAA GCTATTATCT AAGCACGAAT 
TTGCCATTAA CTCAAAAACT TGAAGATAAT GAAAATCAGG TGGTCCATCC ATCAGTCAGA 
GGAAACCAGT GGAGAACCGG ACCATGGGGA TCATGCTCCA GCAGTTGTTC TGGAGGTCTT 
CAGCATAGGG CTGTGGTCTG CCAGGATGAA AATGGACAAA GTGCTAGTTA CTGCGATGCA 
GCCTCCAAGC CTCCAGAGTT ACAGCAATGT GGTCCAGGGC CTTGTCCACA GTGGAACTAC 
GGAAATTGGG GAGAATGTTC ACAAACATGT GGAGGAGGAA TAAAATCAAG ACTTGTAATA 
TGTCAATTTC CCAATGGCCA AATATTAGAA GATCACAACT GTGAAATTGT AAACAAGCCA 
CCTAGCGTAA TACAGTGTCA TATGCATGCT TGCCCTGCTG ATGTGTCATG GCATCAGGAA 
CCATGGACAT CGTGCTCAGC TTCTTGTGGT AAAGGTAGAA AGTACCGTGA AGTGTTTTGC 
ATTGACCAAT TCCAAAGGAA ATTAGAAGAC ACAAATTGCA GTCAAGTACA GAAACCTCCA 
ACTCACAAAG CCTGTAGATC TGTCAGATGC CCTTCATGGA AAGCCAATAG CTGGAATGAG 
TGCTCTGTGA CCTGTGGCTC TGGAGTTCAG CAGAGGGATG TATACTGCAG ACTGAAAGGT 
GTTGGTCAGG TGGTTGAAGA AATGTGTGAT CAGTCCACCC GACCTTGTTC TCAGAGGCGA 
TGTTGGAGTC AGGACTGTGT GCAGCACAAG GGGATGGAAA GAGGGAGGCT GAATTGTTCA 
ACATCATGTG AGAGAAAAGA TTCACATCAA CGAATGGAGT GCACAGATAA CCAAATCAGA 
CAAGTGAATG AAATAGTCTA TAATTCTTCA ACCATATCTC TTACATCCAA GAATTGCAGG 
AACCCTCCTT GCAATTACAT TGTGGTAACA GCAGACTCAT CACAGTGTGC AAACAACTGT 
GGATTTAGTT ACAGACAAAG GATTACATAT TGCACCGAGA TCCCATCTAC TAAGAAACAT 
AAGCTCCATC GACTTCGGCC TATAGTTTAT CAAGAATGCC CTGTGGTGCC TTCCTCTCAG 
GTTTACCAAT GCATTAACAG CTGTTTGCAT TTGGCCACTT GGAAAGTTGG AAAATGGAGC 
AAGTGCTCAG TGACTTGTGG AATTGGGATA ATGAAGAGAC AAGTGAAATG CATTACCAAA 
CATGGTTTGT CCAGTGACTT ATGTTTAAAC CATTTAAAAC CAGGTGCTCA AAAGAAATGT 
TATGCCAATG ACTGTAAATC ATTTACCACC TGCAAAGAAA TTCAAGTGAA AAACCACATT 
AGAAAGGATG GTGACTATTA CCTTAACATT AAGGGAAGAA TAATAAAGAT TTATTGTGCA 
GACATGTACT TGGAGAACCC TAAGGAATAT TTAACACTGG TCCAAGGTGA AGAAAACTTT 
TCTGAAGTGT ATGGCTTTAG ACTAAAAAAT CCATATCAAT GTCCTTTTAA TGGGAGTAGA 
AGGGAAGACT GTGAATGTGA CAATGGACAC TTAGCTGCTG GATACACTGT TTTCAGCAAA 
ATAAGAATTG ATCTCACTTC CATGCAAATT AAAACTACGG ACCTTCTTTT TTCCAAAACA 
ATATTTGGAA ATGCAGTTCC ATTTGCCACA GCTGGAGATT GCTACAGTGC TTTCAGATGC 
CCACAGGGAC AGTTTAGCAT TAATTTGTCA GGAACTGGGA TGAAGATATC CAGCACAGCA 
AAGTGGCTCA CTCAGGGGAG TTATACCTCT GTCAGCATAC GAAGATCAGA GGATGGAACT 
AGATTTTTCG GCAAATGTGG AGGGTACTGT GGAAAGTGTC TTCCTCACAT GACTACTGGT 
CTCCCAATTC AAGTCATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association studies in dogs and humans identify ADAMTS20 as a risk variant for cleft lip and palate.
PLoS genetics11(3)e1005059(2015 03)
Wolf ZT,Brand HA,Shaffer JR,Leslie EJ,Arzi B,Willet CE,Cox TC,McHenry T,Narayan N,Feingold E,Wang X,Sliskovic S,Karmi N,Safra N,Sanchez C,Deleyiannis FW,Murray JC,Wade CM,Marazita ML,Bannasch DL

Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease.
Nature genetics45(12)1452-8(2013 Dec)
Lambert JC,Ibrahim-Verbaas CA,Harold D,Naj AC,Sims R,Bellenguez C,DeStafano AL,Bis JC,Beecham GW,Grenier-Boley B,Russo G,Thorton-Wells TA,Jones N,Smith AV,Chouraki V,Thomas C,Ikram MA,Zelenika D,Vardarajan BN,Kamatani Y,Lin CF,Gerrish A,Schmidt H,Kunkle B,Dunstan ML,Ruiz A,Bihoreau MT,Choi SH,Reitz C,Pasquier F,Cruchaga C,Craig D,Amin N,Berr C,Lopez OL,De Jager PL,Deramecourt V,Johnston JA,Evans D,Lovestone S,Letenneur L,Mor?n FJ,Rubinsztein DC,Eiriksdottir G,Sleegers K,Goate AM,Fi?vet N,Huentelman MW,Gill M,Brown K,Kamboh MI,Keller L,Barberger-Gateau P,McGuiness B,Larson EB,Green R,Myers AJ,Dufouil C,Todd S,Wallon D,Love S,Rogaeva E,Gallacher J,St George-Hyslop P,Clarimon J,Lleo A,Bayer A,Tsuang DW,Yu L,Tsolaki M,Boss? P,Spalletta G,Proitsi P,Collinge J,Sorbi S,Sanchez-Garcia F,Fox NC,Hardy J,Deniz Naranjo MC,Bosco P,Clarke R,Brayne C,Galimberti D,Mancuso M,Matthews F,,,,,Moebus S,Mecocci P,Del Zompo M,Maier W,Hampel H,Pilotto A,Bullido M,Panza F,Caffarra P,Nacmias B,Gilbert JR,Mayhaus M,Lannefelt L,Hakonarson H,Pichler S,Carrasquillo MM,Ingelsson M,Beekly D,Alvarez V,Zou F,Valladares O,Younkin SG,Coto E,Hamilton-Nelson KL,Gu W,Razquin C,Pastor P,Mateo I,Owen MJ,Faber KM,Jonsson PV,Combarros O,O'Donovan MC,Cantwell LB,Soininen H,Blacker D,Mead S,Mosley TH Jr,Bennett DA,Harris TB,Fratiglioni L,Holmes C,de Bruijn RF,Passmore P,Montine TJ,Bettens K,Rotter JI,Brice A,Morgan K,Foroud TM,Kukull WA,Hannequin D,Powell JF,Nalls MA,Ritchie K,Lunetta KL,Kauwe JS,Boerwinkle E,Riemenschneider M,Boada M,Hiltuenen M,Martin ER,Schmidt R,Rujescu D,Wang LS,Dartigues JF,Mayeux R,Tzourio C,Hofman A,N?then MM,Graff C,Psaty BM,Jones L,Haines JL,Holmans PA,Lathrop M,Pericak-Vance MA,Launer LJ,Farrer LA,van Duijn CM,Van Broeckhoven C,Moskvina V,Seshadri S,Williams J,Schellenberg GD,Amouyel P

Genome-wide mapping for clinically relevant predictors of lamotrigine- and phenytoin-induced hypersensitivity reactions.
Pharmacogenomics13(4)399-405(2012 Mar)
McCormack M,Urban TJ,Shianna KV,Walley N,Pandolfo M,Depondt C,Chaila E,O'Conner GD,Kasperavi?i?t? D,Radtke RA,Heinzen EL,Sisodiya SM,Delanty N,Cavalleri GL

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

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ADAMTS20 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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