ARHGEF5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF5
Entrez Gene ID	7984
Full Name	Rho guanine nucleotide exchange factor 5
Synonyms	GEF5,P60,TIM,TIM1
General protein information	Preferred Names Rho guanine nucleotide exchange factor 5 Names rho guanine nucleotide exchange factor 5 Rho guanine nucleotide exchange factor (GEF) 5 ephexin-3 guanine nucleotide regulatory protein TIM oncogene TIM p60 TIM transforming immortalized mammary oncogene
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q35
Summary	Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008].
Disorder MIM:	600888

mRNA and Protein(s)

mRNA	Protein	Name
XM_017012623.1	XP_016868112.1	rho guanine nucleotide exchange factor 5 isoform X1
NM_005435.3	NP_005426.2	rho guanine nucleotide exchange factor 5
NM_005435.4	NP_005426.2	rho guanine nucleotide exchange factor 5
XM_017012623.2	XP_016868112.1	rho guanine nucleotide exchange factor 5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARHGEF5	NP_005426.2
	ARHGEF5	XP_001094223.2
Canis lupus familiaris (dog)	ARHGEF5	XP_005629580.1
Pan troglodytes (chimpanzee)	ARHGEF5	XP_519460.3
Mus musculus (house mouse)	Arhgef5	NP_598435.1
Rattus norvegicus (Norway rat)	Arhgef5	XP_342677.4
Bos taurus (cattle)	ARHGEF5	NP_001103545.2

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway.
Cal? F, Bharti SK, Di Perna R, Brosh RM Jr, Pisani FM
Nucleic acids research44(2)705-17(2016 Jan)

Rho Guanine Nucleotide Exchange Factor 5 Increases Lung Cancer Cell Tumorigenesis via MMP-2 and Cyclin D1 Upregulation.
He P, Wu W, Yang K, Tan D, Tang M, Liu H, Wu T, Zhang S, Wang H
Molecular cancer therapeutics14(7)1671-9(2015 Jul)

Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling.
Lee HK, Ji S, Park SJ, Choung HW, Choi Y, Lee HJ, Park SY, Park JC
The Journal of biological chemistry290(23)14740-53(2015 Jun)

Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer.
Huang O, Wu D, Xie F, Lin L, Wang X, Jiang M, Li Y, Chen W, Shen K, Hu X
Amino acids47(6)1239-46(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tim protein and DDX11 physically and functionally interact and act in concert to preserve replication fork progression in perturbed conditions.
Title: Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway.

Data indicate that Rho guanine nucleotide exchange factor 5 (ARHGEF5) is expressed in most human lung adenocarcinoma tissues and cell lines.
Title: Rho Guanine Nucleotide Exchange Factor 5 Increases Lung Cancer Cell Tumorigenesis via MMP-2 and Cyclin D1 Upregulation.

Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer.
Title: Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer.

The auto-inhibitory state of Rho guanine nucleotide exchange factor ARHGEF5/TIM can be relieved by targeting its SH3 domain with rationally designed peptide aptamers.
Title: The auto-inhibitory state of Rho guanine nucleotide exchange factor ARHGEF5/TIM can be relieved by targeting its SH3 domain with rationally designed peptide aptamers.

A receiver operating characteristic curve constructed with HSP105 and TIM gave a sensitivity of 54.3% and 95% (38/40) specificity in discriminating esophageal squamous cell carcinoma from matched controls.
Title: Identification of tumor antigens that elicit a humoral immune response in the sera of Chinese esophageal squamous cell carcinoma patients by modified serological proteome analysis.

The following ARHGEF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25392	NM_005435.4 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu97499	XM_017012623.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OHu25392	NM_005435.3	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu97499	XM_017012623.1	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF5 ( NM_005435.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005426.2 Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.

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CloneID	OHu25392
Clone ID Related Accession (Same CDS sequence)	NM_005435.3 , NM_005435.4
Accession Version	NM_005435.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK160365.1, CD674013.1, BF209445.1, BI256663.1, AK123596.1 and BC010046.2. On Aug 4, 2004 this sequence version replaced NM_005435.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160365.1, BC136661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTCAAATTT 
GAGCTGATTG TGTCAGAGGC CTCCTACCTG CGCAGTCTAA ACATAGCTGT GGATCATTTC 
CAACTTTCAA CTTCACTCCG GGCCACACTT TCCAACCAGG AGCACCAATG GCTCTTCTCT 
CGTTTACAGG ATGTGCGAGA CGTCAGCGCC ACGTTCCTTT CAGACCTGGA AGAGAACTTT 
GAGAACAATA TCTTCTCCTT CCAAGTATGT GACGTAGTCC TGAACCACGC CCCAGACTTC 
CGCCGGGTCT ACCTGCCTTA TGTCACCAAC CAGACCTATC AGGAACGCAC CTTCCAGAGC 
CTGATGAATA GCAACAGCAA TTTCCGGGAG GTCTTGGAGA AGCTGGAGAG CGACCCCGTC 
TGCCAGCGCC TTTCCCTCAA GTCCTTTCTG ATTCTGCCCT TCCAACGCAT CACCCGCCTC 
AAACTGCTGC TCCAGAACAT TCTGAAGAGA ACACAGCCTG GCTCCTCGGA GGAGGCAGAG 
GCCACGAAGG CACACCACGC CCTGGAGCAG CTGATCCGGG ACTGCAATAA CAATGTCCAG 
AGTATGCGAC GGACAGAGGA ACTAATCTAC CTGAGCCAGA AGATTGAGTT TGAGTGCAAA 
ATATTCCCGC TCATTTCTCA GTCACGCTGG CTGGTGAAAA GTGGGGAGCT GACAGCCTTG 
GAGTTCAGTG CTTCCCCAGG GCTACGAAGG AAGCTGAACA CGCGTCCAGT CCACCTGCAC 
CTCTTCAATG ACTGTCTGCT GCTGTCTCGG CCCCGAGAGG GTAGCCGATT CCTGGTATTT 
GACCATGCTC CCTTCTCCTC CATTCGGGGG GAAAAGTGTG AAATGAAGCT ACATGGACCT 
CACAAAAACC TGTTCCGACT CTTTCTGCGG CAGAACACTC AGGGCGCCCA GGCCGAGTTC 
CTCTTCCGCA CGGAGACTCA AAGTGAAAAG CTTCGGTGGA TCTCAGCCTT GGCCATGCCA 
AGAGAGGAGT TGGACCTTCT GGAGTGTTAC AACTCCCCCC AGGTACAGTG CCTTCGAGCC 
TACAAGCCCC GAGAGAATGA TGAATTGGCA CTGGAGAAAG CCGACGTGGT GATGGTGACT 
CAGCAGAGCA GTGACGGCTG GCTGGAGGGC GTGAGGCTCT CAGACGGGGA GCGAGGCTGG 
TTTCCTGTGC AGCAGGTGGA GTTCATTTCC AACCCAGAGG TCCGTGCACA GAACCTGAAG 
GAAGCTCATC GAGTCAAGAC TGCCAAACTA CAGCTGGTGG AACAGCAAGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005426.2
CDS	119..4912
Translation	MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Target ORF information:

RefSeq Version	NM_005435.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005435.3

ORF Insert Sequence:

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTCAAATTT 
GAGCTGATTG TGTCAGAGGC CTCCTACCTG CGCAGTCTAA ACATAGCTGT GGATCATTTC 
CAACTTTCAA CTTCACTCCG GGCCACACTT TCCAACCAGG AGCACCAATG GCTCTTCTCT 
CGTTTACAGG ATGTGCGAGA CGTCAGCGCC ACGTTCCTTT CAGACCTGGA AGAGAACTTT 
GAGAACAATA TCTTCTCCTT CCAAGTATGT GACGTAGTCC TGAACCACGC CCCAGACTTC 
CGCCGGGTCT ACCTGCCTTA TGTCACCAAC CAGACCTATC AGGAACGCAC CTTCCAGAGC 
CTGATGAATA GCAACAGCAA TTTCCGGGAG GTCTTGGAGA AGCTGGAGAG CGACCCCGTC 
TGCCAGCGCC TTTCCCTCAA GTCCTTTCTG ATTCTGCCCT TCCAACGCAT CACCCGCCTC 
AAACTGCTGC TCCAGAACAT TCTGAAGAGA ACACAGCCTG GCTCCTCGGA GGAGGCAGAG 
GCCACGAAGG CACACCACGC CCTGGAGCAG CTGATCCGGG ACTGCAATAA CAATGTCCAG 
AGTATGCGAC GGACAGAGGA ACTAATCTAC CTGAGCCAGA AGATTGAGTT TGAGTGCAAA 
ATATTCCCGC TCATTTCTCA GTCACGCTGG CTGGTGAAAA GTGGGGAGCT GACAGCCTTG 
GAGTTCAGTG CTTCCCCAGG GCTACGAAGG AAGCTGAACA CGCGTCCAGT CCACCTGCAC 
CTCTTCAATG ACTGTCTGCT GCTGTCTCGG CCCCGAGAGG GTAGCCGATT CCTGGTATTT 
GACCATGCTC CCTTCTCCTC CATTCGGGGG GAAAAGTGTG AAATGAAGCT ACATGGACCT 
CACAAAAACC TGTTCCGACT CTTTCTGCGG CAGAACACTC AGGGCGCCCA GGCCGAGTTC 
CTCTTCCGCA CGGAGACTCA AAGTGAAAAG CTTCGGTGGA TCTCAGCCTT GGCCATGCCA 
AGAGAGGAGT TGGACCTTCT GGAGTGTTAC AACTCCCCCC AGGTACAGTG CCTTCGAGCC 
TACAAGCCCC GAGAGAATGA TGAATTGGCA CTGGAGAAAG CCGACGTGGT GATGGTGACT 
CAGCAGAGCA GTGACGGCTG GCTGGAGGGC GTGAGGCTCT CAGACGGGGA GCGAGGCTGG 
TTTCCTGTGC AGCAGGTGGA GTTCATTTCC AACCCAGAGG TCCGTGCACA GAACCTGAAG 
GAAGCTCATC GAGTCAAGAC TGCCAAACTA CAGCTGGTGG AACAGCAAGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( NM_005435.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005426.2 Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.

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CloneID	OHu25392
Clone ID Related Accession (Same CDS sequence)	NM_005435.3 , NM_005435.4
Accession Version	NM_005435.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK160365.1, CD674013.1, BF209445.1, BI256663.1, AK123596.1 and BC010046.2. On Nov 22, 2018 this sequence version replaced NM_005435.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160365.1, BC136661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000056217.10/ ENSP00000056217.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTCAAATTT 
GAGCTGATTG TGTCAGAGGC CTCCTACCTG CGCAGTCTAA ACATAGCTGT GGATCATTTC 
CAACTTTCAA CTTCACTCCG GGCCACACTT TCCAACCAGG AGCACCAATG GCTCTTCTCT 
CGTTTACAGG ATGTGCGAGA CGTCAGCGCC ACGTTCCTTT CAGACCTGGA AGAGAACTTT 
GAGAACAATA TCTTCTCCTT CCAAGTATGT GACGTAGTCC TGAACCACGC CCCAGACTTC 
CGCCGGGTCT ACCTGCCTTA TGTCACCAAC CAGACCTATC AGGAACGCAC CTTCCAGAGC 
CTGATGAATA GCAACAGCAA TTTCCGGGAG GTCTTGGAGA AGCTGGAGAG CGACCCCGTC 
TGCCAGCGCC TTTCCCTCAA GTCCTTTCTG ATTCTGCCCT TCCAACGCAT CACCCGCCTC 
AAACTGCTGC TCCAGAACAT TCTGAAGAGA ACACAGCCTG GCTCCTCGGA GGAGGCAGAG 
GCCACGAAGG CACACCACGC CCTGGAGCAG CTGATCCGGG ACTGCAATAA CAATGTCCAG 
AGTATGCGAC GGACAGAGGA ACTAATCTAC CTGAGCCAGA AGATTGAGTT TGAGTGCAAA 
ATATTCCCGC TCATTTCTCA GTCACGCTGG CTGGTGAAAA GTGGGGAGCT GACAGCCTTG 
GAGTTCAGTG CTTCCCCAGG GCTACGAAGG AAGCTGAACA CGCGTCCAGT CCACCTGCAC 
CTCTTCAATG ACTGTCTGCT GCTGTCTCGG CCCCGAGAGG GTAGCCGATT CCTGGTATTT 
GACCATGCTC CCTTCTCCTC CATTCGGGGG GAAAAGTGTG AAATGAAGCT ACATGGACCT 
CACAAAAACC TGTTCCGACT CTTTCTGCGG CAGAACACTC AGGGCGCCCA GGCCGAGTTC 
CTCTTCCGCA CGGAGACTCA AAGTGAAAAG CTTCGGTGGA TCTCAGCCTT GGCCATGCCA 
AGAGAGGAGT TGGACCTTCT GGAGTGTTAC AACTCCCCCC AGGTACAGTG CCTTCGAGCC 
TACAAGCCCC GAGAGAATGA TGAATTGGCA CTGGAGAAAG CCGACGTGGT GATGGTGACT 
CAGCAGAGCA GTGACGGCTG GCTGGAGGGC GTGAGGCTCT CAGACGGGGA GCGAGGCTGG 
TTTCCTGTGC AGCAGGTGGA GTTCATTTCC AACCCAGAGG TCCGTGCACA GAACCTGAAG 
GAAGCTCATC GAGTCAAGAC TGCCAAACTA CAGCTGGTGG AACAGCAAGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005426.2
CDS	113..4906
Translation	MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Target ORF information:

RefSeq Version	NM_005435.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005435.4

ORF Insert Sequence:

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTCAAATTT 
GAGCTGATTG TGTCAGAGGC CTCCTACCTG CGCAGTCTAA ACATAGCTGT GGATCATTTC 
CAACTTTCAA CTTCACTCCG GGCCACACTT TCCAACCAGG AGCACCAATG GCTCTTCTCT 
CGTTTACAGG ATGTGCGAGA CGTCAGCGCC ACGTTCCTTT CAGACCTGGA AGAGAACTTT 
GAGAACAATA TCTTCTCCTT CCAAGTATGT GACGTAGTCC TGAACCACGC CCCAGACTTC 
CGCCGGGTCT ACCTGCCTTA TGTCACCAAC CAGACCTATC AGGAACGCAC CTTCCAGAGC 
CTGATGAATA GCAACAGCAA TTTCCGGGAG GTCTTGGAGA AGCTGGAGAG CGACCCCGTC 
TGCCAGCGCC TTTCCCTCAA GTCCTTTCTG ATTCTGCCCT TCCAACGCAT CACCCGCCTC 
AAACTGCTGC TCCAGAACAT TCTGAAGAGA ACACAGCCTG GCTCCTCGGA GGAGGCAGAG 
GCCACGAAGG CACACCACGC CCTGGAGCAG CTGATCCGGG ACTGCAATAA CAATGTCCAG 
AGTATGCGAC GGACAGAGGA ACTAATCTAC CTGAGCCAGA AGATTGAGTT TGAGTGCAAA 
ATATTCCCGC TCATTTCTCA GTCACGCTGG CTGGTGAAAA GTGGGGAGCT GACAGCCTTG 
GAGTTCAGTG CTTCCCCAGG GCTACGAAGG AAGCTGAACA CGCGTCCAGT CCACCTGCAC 
CTCTTCAATG ACTGTCTGCT GCTGTCTCGG CCCCGAGAGG GTAGCCGATT CCTGGTATTT 
GACCATGCTC CCTTCTCCTC CATTCGGGGG GAAAAGTGTG AAATGAAGCT ACATGGACCT 
CACAAAAACC TGTTCCGACT CTTTCTGCGG CAGAACACTC AGGGCGCCCA GGCCGAGTTC 
CTCTTCCGCA CGGAGACTCA AAGTGAAAAG CTTCGGTGGA TCTCAGCCTT GGCCATGCCA 
AGAGAGGAGT TGGACCTTCT GGAGTGTTAC AACTCCCCCC AGGTACAGTG CCTTCGAGCC 
TACAAGCCCC GAGAGAATGA TGAATTGGCA CTGGAGAAAG CCGACGTGGT GATGGTGACT 
CAGCAGAGCA GTGACGGCTG GCTGGAGGGC GTGAGGCTCT CAGACGGGGA GCGAGGCTGG 
TTTCCTGTGC AGCAGGTGGA GTTCATTTCC AACCCAGAGG TCCGTGCACA GAACCTGAAG 
GAAGCTCATC GAGTCAAGAC TGCCAAACTA CAGCTGGTGG AACAGCAAGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_017012623.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868112.1 Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97499
Clone ID Related Accession (Same CDS sequence)	XM_017012623.1 , XM_017012623.2
Accession Version	XM_017012623.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTTCCTTTC 
AGACCTGGAA GAGAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868112.1
CDS	174..3734
Translation	MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVPFRPGREL

Target ORF information:

RefSeq Version	XM_017012623.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012623.1

ORF Insert Sequence:

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTTCCTTTC 
AGACCTGGAA GAGAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_017012623.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868112.1 Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

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CloneID	OHu97499
Clone ID Related Accession (Same CDS sequence)	XM_017012623.1 , XM_017012623.2
Accession Version	XM_017012623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTTCCTTTC 
AGACCTGGAA GAGAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868112.1
CDS	119..3679
Translation	MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVPFRPGREL

Target ORF information:

RefSeq Version	XM_017012623.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012623.2

ORF Insert Sequence:

ATGGAGGCTG AGGAGGCCCA GCGTGGAGCC TCTCCTCCCA TCTCTGCCAT AGAGGAATTC 
AGCATTATCC CTGAGGCTCC CATGAGGAGC AGCCAGGTCT CTGCCTTGGG GCTTGAAGCT 
CAAGAAGATG AGGACCCATC CTATAAGTGG AGAGAGGAAC ACAGACTCTC AGCAACTCAG 
CAGAGTGAGT TAAGGGATGT GTGTGACTAT GCGATTGAGA CGATGCCCTC TTTTCCCAAG 
GAAGGTTCTG CAGATGTGGA GCCCAATCAG GAAAGCCTTG TGGCTGAGGC CTGTGACACT 
CCGGAACACT GGGAGGCAGT ACCCCAGAGC CTAGCAGGCC GACAAGCAAG GACTCTAGCT 
CCCCCAGAGC TCTGGGCCTG CCCCATTCAG AGTGAGCATC TAGACATGGC CCCATTTTCC 
AGTGACCTGG GAAGCGAAGA AGAGGAGGTG GAATTTTGGC CAGGACTTAC TTCTTTGACA 
TTGGGATCTG GACAGGCAGA AGAAGAAGAG GAAACCTCTT CAGATAACTC TGGTCAGACC 
AGATATTATT CTCCCTGCGA AGAGCATCCT GCAGAGACCA ACCAGAATGA AGGCTCTGAA 
AGTGGGACTA TCAGGCAGGG GGAAGAGCTG CCACCTGAGG AGCTGCAGGA AAGTCAAGGG 
CTCTTGCATC CCCAGGAGGT CCAAGTTCTG GAGGAGCAGG GACAGCAGGA AGCAGGATTT 
CGGGGGGAAG GAACTCTGAG GGAGGATGTT TGTGCCGATG GGCTATTAGG GGAGGAACAG 
ATGATAGAGC AGGTTAATGA TGAAAAGGGA GAACAGAAGC AAAAACAGGA ACAGGTACAA 
GATGTGATGC TTGGGAGACA AGGAGAAAGA ATGGGGCTCA CTGGGGAGCC AGAGGGTCTG 
AATGACGGTG AGTGGGAGCA GGAGGATATG GAGAGGAAGG CTCAGGGTCA GGGAGGTCCA 
GAACAGGGAG AAGAGAGGAA GAGGGAGCTG CAGGTGCCAG AAGAGAATAG GGCGGACTCT 
CAGGACGAAA AGAGTCAAAC CTTTTTGGGA AAATCAGAGG AAGTAACTGG AAAGCAAGAA 
GATCATGGTA TAAAGGAGAA AGGGGTGCCA GTCAGCGGGC AGGAGGCGAA AGAGCCAGAG 
AGTTGGGATG GGGGCAGGCT GGGGGCAGTG GGAAGAGCGA GGAGCAGGGA AGAGGAGAAT 
GAGCATCATG GGCCTTCAAT GCCCGCTCTG ATAGCCCCTG AGGACTCTCC TCACTGTGAC 
CTGTTTCCAG GTGCCTCATA TCTCATGACT CAGATTCCCG GGACTCAGAC AGAGTCCAGG 
GCTGAGGAAC TGTCCCCCGC AGCTCTGTCT CCCTCGCTAG AGCCCATCAG GTGCTCTCAC 
CAGCCCATTT CTCTACTGGG CTCCTTTTTG ACTGAGGAGT CACCTGACAA GGAAATAGAT 
CAAAACAGCC AGCAAGAGGA ATCCAGGCTG AGGAAGGGAA CAGTGTCCAG CCAAGGGACT 
GAGGTGGTCT TTGCCAGTGC ATCTGTGACT CCTCCAAGGA CACCAGATTC AGCTCCTCCC 
AGTCCTGCTG AAGCCTACCC CATCACACCT GCCTCGGTAT CTGCCAGGCC CCCAGTTGCC 
TTTCCCAGGA GGGAAACCTC TTGTGCTGCA CGTGCTCCAG AAACTGCCAG TGCCCCTCTC 
TCAATGGATG ACCCATCTCC CTGTGGGACT TCTGAGATGT GCCCGGCTGC CCTCTATGGC 
TTCCCCTCCA CCGGGACCAG CCCTCCGAGG CCCCCAGCCA ACTCCACAGG CACCGTCCAG 
CACTTACGGA GTGACTCCTT CCCTGGTTCT CACAGGACAG AGCAGACTCC AGACCTGGTG 
GGAATGTTGC TTTCCTACTC CCACTCAGAG CTGCCCCAGA GGCCCCCCAA ACCTGCCATC 
TACAGCTCTG TGACCCCAAG AAGGGACAGA AGGAGTGGTA GGGACTACAG CACCGTTTCA 
GCATCCCCTA CTGCCTTATC CACGCTGAAG CAGGACTCTC AAGAATCCAT CTCAAATCTA 
GAGAGACCCA GCAGTCCTCC CAGCATCCAG CCCTGGGTCT CCCCACATAA TCCAGCCTTT 
GCCACAGAGT CTCCCGCCTA CGGTTCTTCC CCATCCTTTG TCTCCATGGA GGATGTGAGG 
ATCCACGAAC CTCTGCCCCC TCCTCCCCCA CAGAGGAGGG ACACCCATCC CTCCGTGGTG 
GAGACAGATG GCCATGCTCG TGTAGTGGTT CCCACGCTGA AGCAGCATAG CCACCCTCCT 
CCATTGGCCC TAGGTTCAGG GCTGCATGCC CCCCATAAAG GCCCACTTCC CCAAGCCTCT 
GACCCCGCTG TGGCCAGGCA GCACCGACCT CTGCCATCTA CCCCAGACAG CTCCCACCAT 
GCTCAGGCCA CCCCCAGGTG GAGATACAAC AAGCCGCTAC CCCCTACCCC TGATTTGCCG 
CAGCCCCACC TTCCTCCCAT TTCTGCTCCT GGTAGCTCAA GGATCTACAG GCCTCTACCC 
CCACTACCCA TCATAGACCC TCCCACCGAA CCACCCCCAT TGCCCCCAAA GTCCAGGGGG 
AGGAGCAGGA GCACTCGGGG AGGACATATG AACTCAGGGG GTCATGCCAA AACAAGACCT 
GCTTGTCAAG ACTGGACAGT CCCCCTCCCT GCCTCTGCTG GACGCACCTC CTGGCCCCCG 
GCCACAGCTA GATCAACAGA GTCTTTCACT TCCACCAGCA GGAGTAAGAG CGAAGTGTCC 
CCTGGCATGG CTTTCAGCAA CATGACAAAC TTCCTATGCC CCTCTTCCCC TACCACTCCC 
TGGACTCCGG AGCTCCAGGG ACCCACCTCT AAGGATGAAG CAGGGGTCTC AGAACACCCT 
GAGGCCCCTG CGAGAGAACC TTTGAGAAGG ACAACCCCTC AGCAAGGAGC CAGTGGCCCA 
GGGAGGTCAC CTGTGGGCCA AGCAAGGCAG CCAGAAAAAC CCAGCCATCT GCACCTGGAG 
AAGGCGTCCA GCTGGCCCCA CAGGCGGGAC TCAGGGAGGC CACCAGGGGA CAGCAGTGGA 
CAGGCTGTGG CTCCTAGTGA GGGGGCCAAC AAGCACAAGG GCTGGAGCCG GCAGGGCCTG 
CGCAGACCTT CCATCTTGCC TGAGGGCTCT TCAGATTCAA GAGGTCCAGC CGTGGAGAAA 
CATCCGGGAC CCTCAGACAC TGTTGTTTTT CGGGAGAAAA AACCAAAGGA GGTGATGGGA 
GGCTTTTCAA GACGCTGCTC CAAACTCATC AACTCCTCCC AGCTGCTTTA CCAGGAGTAT 
AGTGATGTTG TCCTGAATAA GGAGATCCAG AGCCAGCAGC GGCTGGAGAG CCTGTCCGAG 
ACACCCGGGC CTAGCTCTCC GCGGCAGCCT CGGAAGGCCC TGGTCTCCTC CGAGTCGTAC 
CTGCAGCGGC TCTCCATGGC CTCCAGCGGC TCCCTCTGGC AGGAAATCCC CGTGGTGCGC 
AACAGCACCG TGCTGCTCTC CATGACCCAT GAAGACCAAA AGCTGCAAGA GGTTCCTTTC 
AGACCTGGAA GAGAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway.
Nucleic acids research44(2)705-17(2016 Jan)
Cal? F,Bharti SK,Di Perna R,Brosh RM Jr,Pisani FM

Rho Guanine Nucleotide Exchange Factor 5 Increases Lung Cancer Cell Tumorigenesis via MMP-2 and Cyclin D1 Upregulation.
Molecular cancer therapeutics14(7)1671-9(2015 Jul)
He P,Wu W,Yang K,Tan D,Tang M,Liu H,Wu T,Zhang S,Wang H

Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling.
The Journal of biological chemistry290(23)14740-53(2015 Jun)
Lee HK,Ji S,Park SJ,Choung HW,Choi Y,Lee HJ,Park SY,Park JC

Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer.
Amino acids47(6)1239-46(2015 Jun)
Huang O,Wu D,Xie F,Lin L,Wang X,Jiang M,Li Y,Chen W,Shen K,Hu X

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ARHGEF5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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