FBXO31 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FBXO31
Entrez Gene ID	79791
Full Name	F-box protein 31
Synonyms	FBX14,FBXO14,Fbx31,MRT45,pp2386
General protein information	Preferred Names F-box protein 31 Names F-box only protein 31 SCF ubiquitin ligase specificity factor putative breast cancer tumor-suppressor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q24.2
Summary	This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013].
Disorder MIM:	609102
Disorder Html:	?Mental retardation, autosomal recessive 45, 615979 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282683.1	NP_001269612.1	F-box only protein 31 isoform 2
NM_024735.5	NP_079011.3	F-box only protein 31 isoform 1
NM_024735.4	NP_079011.3	F-box only protein 31 isoform 1
NM_001282683.2	NP_001269612.1	F-box only protein 31 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FBXO31	XP_536757.3
Rattus norvegicus (Norway rat)	Fbxo31	NP_001037724.2
Homo sapiens (human)	FBXO31	NP_079011.3
	FBXO31	XP_001086503.1
Gallus gallus (chicken)	FBXO31	XP_414190.4
Gallus gallus (chicken)	LOC100857445	XP_003643231.2
Gallus gallus (chicken)	LOC100858922	XP_003643710.2
Xenopus tropicalis (tropical clawed frog)	fbxo31	NP_001072525.1
Pan troglodytes (chimpanzee)	FBXO31	XP_001156413.1
Mus musculus (house mouse)	Fbxo31	NP_598526.3
Bos taurus (cattle)	FBXO31	XP_002694899.3
Danio rerio (zebrafish)	si:ch211-147k9.7	XP_005170757.1

Gene Ontology
Bibliography

Related articles in PubMed

Degradation of FBXO31 by APC/C is regulated by AKT- and ATM-mediated phosphorylation.
Choppara S, Malonia SK, Sankaran G, Green MR, Santra MK
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

Structural basis of the phosphorylation-independent recognition of cyclin D1 by the SCF
Li Y, Jin K, Bunker E, Zhang X, Luo X, Liu X, Hao B
Proceedings of the National Academy of Sciences of the United States of America115(2)319-324(2018 Jan)

Overexpression of F-box only protein 31 predicts poor prognosis and deregulates p38?- and JNK-mediated apoptosis in esophageal squamous cell carcinoma.
Liu J, Lv L, Gong J, Tan Y, Zhu Y, Dai Y, Pan X, Huen MSY, Li B, Tsao SW, Huo J, Cheung ALM
International journal of cancer142(1)145-155(2018 Jan)

FBXO31 Suppresses Gastric Cancer EMT by Targeting Snail1 for Proteasomal Degradation.
Zou S, Ma C, Yang F, Xu X, Jia J, Liu Z
Molecular cancer research : MCR(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in addition to revealing that FBXO31 is an independent prognostic marker for esophageal squamous cell carcinoma , our findings substantiate a novel regulatory role of FBXO31 in tumorigenesis and drug resistance of esophageal squamous cell carcinoma
Title: Overexpression of F-box only protein 31 predicts poor prognosis and deregulates p38?- and JNK-mediated apoptosis in esophageal squamous cell carcinoma.

FBXO31-mediated loss of MDM2 leads to elevated levels of p53, resulting in growth arrest. In cells depleted of FBXO31, MDM2 is not degraded and p53 levels do not increase following genotoxic stress
Title: F-box protein FBXO31 directs degradation of MDM2 to facilitate p53-mediated growth arrest following genotoxic stress.

Expression of FBXO31 inhibited xenograft tumor growth in mice. miR-20a and miR-17 mimics inhibited, whereas the inhibitor of miR-20a and miR-17 increased, the expression of FBXO31, respectively. miR-20a and miR-17 directly bind to the 3'-UTR of FBXO31.
Title: F-box protein FBXO31 is down-regulated in gastric cancer and negatively regulated by miR-17 and miR-20a.

uncover a new mechanism of deactivation of MKK6-p38 and substantiate a novel regulatory role of FBXO31 in stress response
Title: F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6).

FBXO31 interacts with Cdt1 and regulates the degradation of Cdt1 in G2 phase.
Title: SCF-FBXO31 E3 ligase targets DNA replication factor Cdt1 for proteolysis in the G2 phase of cell cycle to prevent re-replication.

The following FBXO31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FBXO31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00854	NM_024735.5 Latest version!	Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu05060	NM_001282683.2 Latest version!	Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu00854	NM_024735.4	Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu05060	NM_001282683.1	Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FBXO31 ( NM_024735.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_079011.3 Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00854
Clone ID Related Accession (Same CDS sequence)	NM_024735.5 , NM_024735.4
Accession Version	NM_024735.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	F-box only protein 31 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI596608.1, BC012748.1, AF318348.1, BM151642.1, BI914636.1, DA493744.1, BX329100.2, AL576585.3, BX106211.1 and BE465371.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024735.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012748.1, AF428140.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000311635.12/ ENSP00000310841.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGTGT GTGCTCGCCT TTGCGGCGTG GGCCCGTCGC GCGGATGTCG GCGCCGCCAG 
CAGCGCCGGG GCCCGGCCGA GACGGCGGCG GCCGACAGCG AGCCGGACAC AGACCCCGAG 
GAGGAGCGCA TCGAGGCTAG CGCCGGGGTC GGGGGCGGCT TGTGCGCGGG CCCCTCGCCG 
CCGCCCCCGC GCTGCTCGCT GCTGGAGCTG CCGCCCGAGC TGCTGGTGGA GATCTTCGCG 
TCGCTGCCGG GCACGGACCT ACCCAGCTTG GCCCAGGTCT GCACGAAGTT CCGGCGCATC 
CTCCACACCG ACACCATCTG GAGGAGGCGT TGCCGTGAGG AGTATGGTGT TTGCGAAAAC 
TTGCGGAAGC TGGAGATCAC AGGCGTGTCT TGTCGGGACG TCTATGCGAA GCTGCTTCAC 
CGATATAGAC ACATTTTGGG ATTGTGGCAG CCAGATATCG GGCCATACGG AGGACTGCTG 
AACGTGGTGG TGGACGGCCT GTTCATCATC GGGTGGATGT ACCTGCCTCC CCATGACCCC 
CACGTCGATG ACCCTATGAG ATTCAAGCCT CTGTTCAGGA TCCACCTGAT GGAGAGGAAG 
GCTGCCACAG TGGAGTGCAT GTACGGCCAC AAAGGGCCCC ACCACGGCCA CATCCAGATT 
GTGAAGAAGG ATGAGTTCTC CACCAAGTGC AACCAGACGG ACCACCACAG GATGTCCGGC 
GGGAGGCAGG AGGAGTTTCG GACGTGGCTG AGGGAGGAAT GGGGGCGCAC GCTGGAGGAC 
ATCTTCCACG AGCACATGCA GGAGCTCATC CTGATGAAGT TCATCTACAC CAGTCAGTAC 
GACAACTGCC TGACCTACCG CCGCATCTAC CTGCCGCCCA GCCGCCCCGA CGACCTCATC 
AAGCCTGGCC TCTTCAAAGG TACCTATGGC AGCCACGGCC TGGAGATTGT GATGCTCAGC 
TTCCACGGCC GGCGTGCCAG GGGCACCAAG ATCACGGGCG ACCCCAACAT CCCCGCTGGG 
CAGCAGACAG TGGAGATCGA CCTGAGGCAT CGGATCCAGC TGCCCGACCT CGAGAACCAG 
CGCAACTTCA ATGAGCTCTC CCGCATCGTC CTGGAGGTGC GCGAGAGGGT GCGCCAGGAG 
CAGCAGGAAG GCGGGCACGA GGCGGGCGAG GGTCGTGGCC GGCAGGGCCC CCGGGAGTCC 
CAGCCAAGCC CTGCCCAGCC CAGGGCAGAG GCGCCCAGCA AGGGCCCAGA TGGGACACCT 
GGTGAGGATG GTGGCGAGCC TGGGGATGCC GTAGCTGCGG CCGAGCAGCC TGCCCAGTGT 
GGGCAGGGGC AGCCGTTCGT GCTGCCCGTG GGCGTGAGCT CCAGGAATGA GGACTACCCC 
CGAACCTGCA GGATGTGTTT TTATGGCACA GGCCTCATCG CGGGCCACGG CTTCACCAGC 
CCTGAACGCA CCCCCGGGGT CTTCATCCTC TTCGATGAGG ACCGCTTCGG GTTCGTCTGG 
CTGGAGCTGA AATCCTTCAG CCTGTACAGC CGGGTCCAGG CCACCTTCCG GAACGCAGAT 
GCGCCGTCCC CACAGGCCTT CGATGAGATG CTCAAGAACA TTCAGTCCCT CACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079011.3
CDS	33..1652
Translation	MAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDLPSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKLLHRYRHILGLWQPDIGPYGGLLNVVVDGLFIIGWMYLPPHDPHVDDPMRFKPLFRIHLMERKAATVECMYGHKGPHHGHIQIVKKDEFSTKCNQTDHHRMSGGRQEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSRPDDLIKPGLFKGTYGSHGLEIVMLSFHGRRARGTKITGDPNIPAGQQTVEIDLRHRIQLPDLENQRNFNELSRIVLEVRERVRQEQQEGGHEAGEGRGRQGPRESQPSPAQPRAEAPSKGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQPFVLPVGVSSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFVWLELKSFSLYSRVQATFRNADAPSPQAFDEMLKNIQSLTS

Target ORF information:

RefSeq Version	NM_024735.5
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024735.5

ORF Insert Sequence:

ATGGCGGTGT GTGCTCGCCT TTGCGGCGTG GGCCCGTCGC GCGGATGTCG GCGCCGCCAG 
CAGCGCCGGG GCCCGGCCGA GACGGCGGCG GCCGACAGCG AGCCGGACAC AGACCCCGAG 
GAGGAGCGCA TCGAGGCTAG CGCCGGGGTC GGGGGCGGCT TGTGCGCGGG CCCCTCGCCG 
CCGCCCCCGC GCTGCTCGCT GCTGGAGCTG CCGCCCGAGC TGCTGGTGGA GATCTTCGCG 
TCGCTGCCGG GCACGGACCT ACCCAGCTTG GCCCAGGTCT GCACGAAGTT CCGGCGCATC 
CTCCACACCG ACACCATCTG GAGGAGGCGT TGCCGTGAGG AGTATGGTGT TTGCGAAAAC 
TTGCGGAAGC TGGAGATCAC AGGCGTGTCT TGTCGGGACG TCTATGCGAA GCTGCTTCAC 
CGATATAGAC ACATTTTGGG ATTGTGGCAG CCAGATATCG GGCCATACGG AGGACTGCTG 
AACGTGGTGG TGGACGGCCT GTTCATCATC GGGTGGATGT ACCTGCCTCC CCATGACCCC 
CACGTCGATG ACCCTATGAG ATTCAAGCCT CTGTTCAGGA TCCACCTGAT GGAGAGGAAG 
GCTGCCACAG TGGAGTGCAT GTACGGCCAC AAAGGGCCCC ACCACGGCCA CATCCAGATT 
GTGAAGAAGG ATGAGTTCTC CACCAAGTGC AACCAGACGG ACCACCACAG GATGTCCGGC 
GGGAGGCAGG AGGAGTTTCG GACGTGGCTG AGGGAGGAAT GGGGGCGCAC GCTGGAGGAC 
ATCTTCCACG AGCACATGCA GGAGCTCATC CTGATGAAGT TCATCTACAC CAGTCAGTAC 
GACAACTGCC TGACCTACCG CCGCATCTAC CTGCCGCCCA GCCGCCCCGA CGACCTCATC 
AAGCCTGGCC TCTTCAAAGG TACCTATGGC AGCCACGGCC TGGAGATTGT GATGCTCAGC 
TTCCACGGCC GGCGTGCCAG GGGCACCAAG ATCACGGGCG ACCCCAACAT CCCCGCTGGG 
CAGCAGACAG TGGAGATCGA CCTGAGGCAT CGGATCCAGC TGCCCGACCT CGAGAACCAG 
CGCAACTTCA ATGAGCTCTC CCGCATCGTC CTGGAGGTGC GCGAGAGGGT GCGCCAGGAG 
CAGCAGGAAG GCGGGCACGA GGCGGGCGAG GGTCGTGGCC GGCAGGGCCC CCGGGAGTCC 
CAGCCAAGCC CTGCCCAGCC CAGGGCAGAG GCGCCCAGCA AGGGCCCAGA TGGGACACCT 
GGTGAGGATG GTGGCGAGCC TGGGGATGCC GTAGCTGCGG CCGAGCAGCC TGCCCAGTGT 
GGGCAGGGGC AGCCGTTCGT GCTGCCCGTG GGCGTGAGCT CCAGGAATGA GGACTACCCC 
CGAACCTGCA GGATGTGTTT TTATGGCACA GGCCTCATCG CGGGCCACGG CTTCACCAGC 
CCTGAACGCA CCCCCGGGGT CTTCATCCTC TTCGATGAGG ACCGCTTCGG GTTCGTCTGG 
CTGGAGCTGA AATCCTTCAG CCTGTACAGC CGGGTCCAGG CCACCTTCCG GAACGCAGAT 
GCGCCGTCCC CACAGGCCTT CGATGAGATG CTCAAGAACA TTCAGTCCCT CACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO31 ( NM_024735.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079011.3 Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00854
Clone ID Related Accession (Same CDS sequence)	NM_024735.5 , NM_024735.4
Accession Version	NM_024735.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	F-box only protein 31 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010531.8, BI596608.1, BC012748.1, AF318348.1, BM151642.1, BI914636.1, DA493744.1, BX329100.2, AL576585.3, BX106211.1 and BE465371.1. This sequence is a reference standard in the RefSeqGene project. On Sep 18, 2013 this sequence version replaced NM_024735.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012748.1, AF428140.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGT GTGCTCGCCT TTGCGGCGTG GGCCCGTCGC GCGGATGTCG GCGCCGCCAG 
CAGCGCCGGG GCCCGGCCGA GACGGCGGCG GCCGACAGCG AGCCGGACAC AGACCCCGAG 
GAGGAGCGCA TCGAGGCTAG CGCCGGGGTC GGGGGCGGCT TGTGCGCGGG CCCCTCGCCG 
CCGCCCCCGC GCTGCTCGCT GCTGGAGCTG CCGCCCGAGC TGCTGGTGGA GATCTTCGCG 
TCGCTGCCGG GCACGGACCT ACCCAGCTTG GCCCAGGTCT GCACGAAGTT CCGGCGCATC 
CTCCACACCG ACACCATCTG GAGGAGGCGT TGCCGTGAGG AGTATGGTGT TTGCGAAAAC 
TTGCGGAAGC TGGAGATCAC AGGCGTGTCT TGTCGGGACG TCTATGCGAA GCTGCTTCAC 
CGATATAGAC ACATTTTGGG ATTGTGGCAG CCAGATATCG GGCCATACGG AGGACTGCTG 
AACGTGGTGG TGGACGGCCT GTTCATCATC GGGTGGATGT ACCTGCCTCC CCATGACCCC 
CACGTCGATG ACCCTATGAG ATTCAAGCCT CTGTTCAGGA TCCACCTGAT GGAGAGGAAG 
GCTGCCACAG TGGAGTGCAT GTACGGCCAC AAAGGGCCCC ACCACGGCCA CATCCAGATT 
GTGAAGAAGG ATGAGTTCTC CACCAAGTGC AACCAGACGG ACCACCACAG GATGTCCGGC 
GGGAGGCAGG AGGAGTTTCG GACGTGGCTG AGGGAGGAAT GGGGGCGCAC GCTGGAGGAC 
ATCTTCCACG AGCACATGCA GGAGCTCATC CTGATGAAGT TCATCTACAC CAGTCAGTAC 
GACAACTGCC TGACCTACCG CCGCATCTAC CTGCCGCCCA GCCGCCCCGA CGACCTCATC 
AAGCCTGGCC TCTTCAAAGG TACCTATGGC AGCCACGGCC TGGAGATTGT GATGCTCAGC 
TTCCACGGCC GGCGTGCCAG GGGCACCAAG ATCACGGGCG ACCCCAACAT CCCCGCTGGG 
CAGCAGACAG TGGAGATCGA CCTGAGGCAT CGGATCCAGC TGCCCGACCT CGAGAACCAG 
CGCAACTTCA ATGAGCTCTC CCGCATCGTC CTGGAGGTGC GCGAGAGGGT GCGCCAGGAG 
CAGCAGGAAG GCGGGCACGA GGCGGGCGAG GGTCGTGGCC GGCAGGGCCC CCGGGAGTCC 
CAGCCAAGCC CTGCCCAGCC CAGGGCAGAG GCGCCCAGCA AGGGCCCAGA TGGGACACCT 
GGTGAGGATG GTGGCGAGCC TGGGGATGCC GTAGCTGCGG CCGAGCAGCC TGCCCAGTGT 
GGGCAGGGGC AGCCGTTCGT GCTGCCCGTG GGCGTGAGCT CCAGGAATGA GGACTACCCC 
CGAACCTGCA GGATGTGTTT TTATGGCACA GGCCTCATCG CGGGCCACGG CTTCACCAGC 
CCTGAACGCA CCCCCGGGGT CTTCATCCTC TTCGATGAGG ACCGCTTCGG GTTCGTCTGG 
CTGGAGCTGA AATCCTTCAG CCTGTACAGC CGGGTCCAGG CCACCTTCCG GAACGCAGAT 
GCGCCGTCCC CACAGGCCTT CGATGAGATG CTCAAGAACA TTCAGTCCCT CACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079011.3
CDS	50..1669
Translation	MAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDLPSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKLLHRYRHILGLWQPDIGPYGGLLNVVVDGLFIIGWMYLPPHDPHVDDPMRFKPLFRIHLMERKAATVECMYGHKGPHHGHIQIVKKDEFSTKCNQTDHHRMSGGRQEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSRPDDLIKPGLFKGTYGSHGLEIVMLSFHGRRARGTKITGDPNIPAGQQTVEIDLRHRIQLPDLENQRNFNELSRIVLEVRERVRQEQQEGGHEAGEGRGRQGPRESQPSPAQPRAEAPSKGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQPFVLPVGVSSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFVWLELKSFSLYSRVQATFRNADAPSPQAFDEMLKNIQSLTS

Target ORF information:

RefSeq Version	NM_024735.4
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 31 (FBXO31), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024735.4

ORF Insert Sequence:

ATGGCGGTGT GTGCTCGCCT TTGCGGCGTG GGCCCGTCGC GCGGATGTCG GCGCCGCCAG 
CAGCGCCGGG GCCCGGCCGA GACGGCGGCG GCCGACAGCG AGCCGGACAC AGACCCCGAG 
GAGGAGCGCA TCGAGGCTAG CGCCGGGGTC GGGGGCGGCT TGTGCGCGGG CCCCTCGCCG 
CCGCCCCCGC GCTGCTCGCT GCTGGAGCTG CCGCCCGAGC TGCTGGTGGA GATCTTCGCG 
TCGCTGCCGG GCACGGACCT ACCCAGCTTG GCCCAGGTCT GCACGAAGTT CCGGCGCATC 
CTCCACACCG ACACCATCTG GAGGAGGCGT TGCCGTGAGG AGTATGGTGT TTGCGAAAAC 
TTGCGGAAGC TGGAGATCAC AGGCGTGTCT TGTCGGGACG TCTATGCGAA GCTGCTTCAC 
CGATATAGAC ACATTTTGGG ATTGTGGCAG CCAGATATCG GGCCATACGG AGGACTGCTG 
AACGTGGTGG TGGACGGCCT GTTCATCATC GGGTGGATGT ACCTGCCTCC CCATGACCCC 
CACGTCGATG ACCCTATGAG ATTCAAGCCT CTGTTCAGGA TCCACCTGAT GGAGAGGAAG 
GCTGCCACAG TGGAGTGCAT GTACGGCCAC AAAGGGCCCC ACCACGGCCA CATCCAGATT 
GTGAAGAAGG ATGAGTTCTC CACCAAGTGC AACCAGACGG ACCACCACAG GATGTCCGGC 
GGGAGGCAGG AGGAGTTTCG GACGTGGCTG AGGGAGGAAT GGGGGCGCAC GCTGGAGGAC 
ATCTTCCACG AGCACATGCA GGAGCTCATC CTGATGAAGT TCATCTACAC CAGTCAGTAC 
GACAACTGCC TGACCTACCG CCGCATCTAC CTGCCGCCCA GCCGCCCCGA CGACCTCATC 
AAGCCTGGCC TCTTCAAAGG TACCTATGGC AGCCACGGCC TGGAGATTGT GATGCTCAGC 
TTCCACGGCC GGCGTGCCAG GGGCACCAAG ATCACGGGCG ACCCCAACAT CCCCGCTGGG 
CAGCAGACAG TGGAGATCGA CCTGAGGCAT CGGATCCAGC TGCCCGACCT CGAGAACCAG 
CGCAACTTCA ATGAGCTCTC CCGCATCGTC CTGGAGGTGC GCGAGAGGGT GCGCCAGGAG 
CAGCAGGAAG GCGGGCACGA GGCGGGCGAG GGTCGTGGCC GGCAGGGCCC CCGGGAGTCC 
CAGCCAAGCC CTGCCCAGCC CAGGGCAGAG GCGCCCAGCA AGGGCCCAGA TGGGACACCT 
GGTGAGGATG GTGGCGAGCC TGGGGATGCC GTAGCTGCGG CCGAGCAGCC TGCCCAGTGT 
GGGCAGGGGC AGCCGTTCGT GCTGCCCGTG GGCGTGAGCT CCAGGAATGA GGACTACCCC 
CGAACCTGCA GGATGTGTTT TTATGGCACA GGCCTCATCG CGGGCCACGG CTTCACCAGC 
CCTGAACGCA CCCCCGGGGT CTTCATCCTC TTCGATGAGG ACCGCTTCGG GTTCGTCTGG 
CTGGAGCTGA AATCCTTCAG CCTGTACAGC CGGGTCCAGG CCACCTTCCG GAACGCAGAT 
GCGCCGTCCC CACAGGCCTT CGATGAGATG CTCAAGAACA TTCAGTCCCT CACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO31 ( NM_001282683.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269612.1 Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05060
Clone ID Related Accession (Same CDS sequence)	NM_001282683.1 , NM_001282683.2
Accession Version	NM_001282683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	F-box only protein 31 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB444853.1, BG718342.1, BC012748.1, AL117444.1, BM151642.1, BI914636.1, DA493744.1, BX329100.2, AL576585.3, BX106211.1 and BE465371.1. On Sep 18, 2013 this sequence version replaced NR_024568.1. Transcript Variant: This variant (2) represents use of an alternate promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC012748.1, SRR1803612.135580.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCTGC CTCCCCATGA CCCCCACGTC GATGACCCTA TGAGATTCAA GCCTCTGTTC 
AGGATCCACC TGATGGAGAG GAAGGCTGCC ACAGTGGAGT GCATGTACGG CCACAAAGGG 
CCCCACCACG GCCACATCCA GATTGTGAAG AAGGATGAGT TCTCCACCAA GTGCAACCAG 
ACGGACCACC ACAGGATGTC CGGCGGGAGG CAGGAGGAGT TTCGGACGTG GCTGAGGGAG 
GAATGGGGGC GCACGCTGGA GGACATCTTC CACGAGCACA TGCAGGAGCT CATCCTGATG 
AAGTTCATCT ACACCAGTCA GTACGACAAC TGCCTGACCT ACCGCCGCAT CTACCTGCCG 
CCCAGCCGCC CCGACGACCT CATCAAGCCT GGCCTCTTCA AAGGTACCTA TGGCAGCCAC 
GGCCTGGAGA TTGTGATGCT CAGCTTCCAC GGCCGGCGTG CCAGGGGCAC CAAGATCACG 
GGCGACCCCA ACATCCCCGC TGGGCAGCAG ACAGTGGAGA TCGACCTGAG GCATCGGATC 
CAGCTGCCCG ACCTCGAGAA CCAGCGCAAC TTCAATGAGC TCTCCCGCAT CGTCCTGGAG 
GTGCGCGAGA GGGTGCGCCA GGAGCAGCAG GAAGGCGGGC ACGAGGCGGG CGAGGGTCGT 
GGCCGGCAGG GCCCCCGGGA GTCCCAGCCA AGCCCTGCCC AGCCCAGGGC AGAGGCGCCC 
AGCAAGGGCC CAGATGGGAC ACCTGGTGAG GATGGTGGCG AGCCTGGGGA TGCCGTAGCT 
GCGGCCGAGC AGCCTGCCCA GTGTGGGCAG GGGCAGCCGT TCGTGCTGCC CGTGGGCGTG 
AGCTCCAGGA ATGAGGACTA CCCCCGAACC TGCAGGATGT GTTTTTATGG CACAGGCCTC 
ATCGCGGGCC ACGGCTTCAC CAGCCCTGAA CGCACCCCCG GGGTCTTCAT CCTCTTCGAT 
GAGGACCGCT TCGGGTTCGT CTGGCTGGAG CTGAAATCCT TCAGCCTGTA CAGCCGGGTC 
CAGGCCACCT TCCGGAACGC AGATGCGCCG TCCCCACAGG CCTTCGATGA GATGCTCAAG 
AACATTCAGT CCCTCACCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269612.1
CDS	314..1417
Translation	MYLPPHDPHVDDPMRFKPLFRIHLMERKAATVECMYGHKGPHHGHIQIVKKDEFSTKCNQTDHHRMSGGRQEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSRPDDLIKPGLFKGTYGSHGLEIVMLSFHGRRARGTKITGDPNIPAGQQTVEIDLRHRIQLPDLENQRNFNELSRIVLEVRERVRQEQQEGGHEAGEGRGRQGPRESQPSPAQPRAEAPSKGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQPFVLPVGVSSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFVWLELKSFSLYSRVQATFRNADAPSPQAFDEMLKNIQSLTS

Target ORF information:

RefSeq Version	NM_001282683.1
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282683.1

ORF Insert Sequence:

ATGTACCTGC CTCCCCATGA CCCCCACGTC GATGACCCTA TGAGATTCAA GCCTCTGTTC 
AGGATCCACC TGATGGAGAG GAAGGCTGCC ACAGTGGAGT GCATGTACGG CCACAAAGGG 
CCCCACCACG GCCACATCCA GATTGTGAAG AAGGATGAGT TCTCCACCAA GTGCAACCAG 
ACGGACCACC ACAGGATGTC CGGCGGGAGG CAGGAGGAGT TTCGGACGTG GCTGAGGGAG 
GAATGGGGGC GCACGCTGGA GGACATCTTC CACGAGCACA TGCAGGAGCT CATCCTGATG 
AAGTTCATCT ACACCAGTCA GTACGACAAC TGCCTGACCT ACCGCCGCAT CTACCTGCCG 
CCCAGCCGCC CCGACGACCT CATCAAGCCT GGCCTCTTCA AAGGTACCTA TGGCAGCCAC 
GGCCTGGAGA TTGTGATGCT CAGCTTCCAC GGCCGGCGTG CCAGGGGCAC CAAGATCACG 
GGCGACCCCA ACATCCCCGC TGGGCAGCAG ACAGTGGAGA TCGACCTGAG GCATCGGATC 
CAGCTGCCCG ACCTCGAGAA CCAGCGCAAC TTCAATGAGC TCTCCCGCAT CGTCCTGGAG 
GTGCGCGAGA GGGTGCGCCA GGAGCAGCAG GAAGGCGGGC ACGAGGCGGG CGAGGGTCGT 
GGCCGGCAGG GCCCCCGGGA GTCCCAGCCA AGCCCTGCCC AGCCCAGGGC AGAGGCGCCC 
AGCAAGGGCC CAGATGGGAC ACCTGGTGAG GATGGTGGCG AGCCTGGGGA TGCCGTAGCT 
GCGGCCGAGC AGCCTGCCCA GTGTGGGCAG GGGCAGCCGT TCGTGCTGCC CGTGGGCGTG 
AGCTCCAGGA ATGAGGACTA CCCCCGAACC TGCAGGATGT GTTTTTATGG CACAGGCCTC 
ATCGCGGGCC ACGGCTTCAC CAGCCCTGAA CGCACCCCCG GGGTCTTCAT CCTCTTCGAT 
GAGGACCGCT TCGGGTTCGT CTGGCTGGAG CTGAAATCCT TCAGCCTGTA CAGCCGGGTC 
CAGGCCACCT TCCGGAACGC AGATGCGCCG TCCCCACAGG CCTTCGATGA GATGCTCAAG 
AACATTCAGT CCCTCACCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO31 ( NM_001282683.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269612.1 Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.

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CloneID	OHu05060
Clone ID Related Accession (Same CDS sequence)	NM_001282683.1 , NM_001282683.2
Accession Version	NM_001282683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	F-box only protein 31 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010531.8, DB444853.1, BG718342.1, BC012748.1, AL117444.1, BM151642.1, BI914636.1, DA493744.1, BX329100.2, AL576585.3, BX106211.1 and BE465371.1. On Jun 1, 2019 this sequence version replaced NM_001282683.1. Transcript Variant: This variant (2) represents use of an alternate promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660809.185952.1, SRR1660803.242164.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCTGC CTCCCCATGA CCCCCACGTC GATGACCCTA TGAGATTCAA GCCTCTGTTC 
AGGATCCACC TGATGGAGAG GAAGGCTGCC ACAGTGGAGT GCATGTACGG CCACAAAGGG 
CCCCACCACG GCCACATCCA GATTGTGAAG AAGGATGAGT TCTCCACCAA GTGCAACCAG 
ACGGACCACC ACAGGATGTC CGGCGGGAGG CAGGAGGAGT TTCGGACGTG GCTGAGGGAG 
GAATGGGGGC GCACGCTGGA GGACATCTTC CACGAGCACA TGCAGGAGCT CATCCTGATG 
AAGTTCATCT ACACCAGTCA GTACGACAAC TGCCTGACCT ACCGCCGCAT CTACCTGCCG 
CCCAGCCGCC CCGACGACCT CATCAAGCCT GGCCTCTTCA AAGGTACCTA TGGCAGCCAC 
GGCCTGGAGA TTGTGATGCT CAGCTTCCAC GGCCGGCGTG CCAGGGGCAC CAAGATCACG 
GGCGACCCCA ACATCCCCGC TGGGCAGCAG ACAGTGGAGA TCGACCTGAG GCATCGGATC 
CAGCTGCCCG ACCTCGAGAA CCAGCGCAAC TTCAATGAGC TCTCCCGCAT CGTCCTGGAG 
GTGCGCGAGA GGGTGCGCCA GGAGCAGCAG GAAGGCGGGC ACGAGGCGGG CGAGGGTCGT 
GGCCGGCAGG GCCCCCGGGA GTCCCAGCCA AGCCCTGCCC AGCCCAGGGC AGAGGCGCCC 
AGCAAGGGCC CAGATGGGAC ACCTGGTGAG GATGGTGGCG AGCCTGGGGA TGCCGTAGCT 
GCGGCCGAGC AGCCTGCCCA GTGTGGGCAG GGGCAGCCGT TCGTGCTGCC CGTGGGCGTG 
AGCTCCAGGA ATGAGGACTA CCCCCGAACC TGCAGGATGT GTTTTTATGG CACAGGCCTC 
ATCGCGGGCC ACGGCTTCAC CAGCCCTGAA CGCACCCCCG GGGTCTTCAT CCTCTTCGAT 
GAGGACCGCT TCGGGTTCGT CTGGCTGGAG CTGAAATCCT TCAGCCTGTA CAGCCGGGTC 
CAGGCCACCT TCCGGAACGC AGATGCGCCG TCCCCACAGG CCTTCGATGA GATGCTCAAG 
AACATTCAGT CCCTCACCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269612.1
CDS	328..1431
Translation	MYLPPHDPHVDDPMRFKPLFRIHLMERKAATVECMYGHKGPHHGHIQIVKKDEFSTKCNQTDHHRMSGGRQEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSRPDDLIKPGLFKGTYGSHGLEIVMLSFHGRRARGTKITGDPNIPAGQQTVEIDLRHRIQLPDLENQRNFNELSRIVLEVRERVRQEQQEGGHEAGEGRGRQGPRESQPSPAQPRAEAPSKGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQPFVLPVGVSSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFVWLELKSFSLYSRVQATFRNADAPSPQAFDEMLKNIQSLTS

Target ORF information:

RefSeq Version	NM_001282683.2
Organism	Homo sapiens(human)
Definition	Homo sapiens F-box protein 31 (FBXO31), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282683.2

ORF Insert Sequence:

ATGTACCTGC CTCCCCATGA CCCCCACGTC GATGACCCTA TGAGATTCAA GCCTCTGTTC 
AGGATCCACC TGATGGAGAG GAAGGCTGCC ACAGTGGAGT GCATGTACGG CCACAAAGGG 
CCCCACCACG GCCACATCCA GATTGTGAAG AAGGATGAGT TCTCCACCAA GTGCAACCAG 
ACGGACCACC ACAGGATGTC CGGCGGGAGG CAGGAGGAGT TTCGGACGTG GCTGAGGGAG 
GAATGGGGGC GCACGCTGGA GGACATCTTC CACGAGCACA TGCAGGAGCT CATCCTGATG 
AAGTTCATCT ACACCAGTCA GTACGACAAC TGCCTGACCT ACCGCCGCAT CTACCTGCCG 
CCCAGCCGCC CCGACGACCT CATCAAGCCT GGCCTCTTCA AAGGTACCTA TGGCAGCCAC 
GGCCTGGAGA TTGTGATGCT CAGCTTCCAC GGCCGGCGTG CCAGGGGCAC CAAGATCACG 
GGCGACCCCA ACATCCCCGC TGGGCAGCAG ACAGTGGAGA TCGACCTGAG GCATCGGATC 
CAGCTGCCCG ACCTCGAGAA CCAGCGCAAC TTCAATGAGC TCTCCCGCAT CGTCCTGGAG 
GTGCGCGAGA GGGTGCGCCA GGAGCAGCAG GAAGGCGGGC ACGAGGCGGG CGAGGGTCGT 
GGCCGGCAGG GCCCCCGGGA GTCCCAGCCA AGCCCTGCCC AGCCCAGGGC AGAGGCGCCC 
AGCAAGGGCC CAGATGGGAC ACCTGGTGAG GATGGTGGCG AGCCTGGGGA TGCCGTAGCT 
GCGGCCGAGC AGCCTGCCCA GTGTGGGCAG GGGCAGCCGT TCGTGCTGCC CGTGGGCGTG 
AGCTCCAGGA ATGAGGACTA CCCCCGAACC TGCAGGATGT GTTTTTATGG CACAGGCCTC 
ATCGCGGGCC ACGGCTTCAC CAGCCCTGAA CGCACCCCCG GGGTCTTCAT CCTCTTCGAT 
GAGGACCGCT TCGGGTTCGT CTGGCTGGAG CTGAAATCCT TCAGCCTGTA CAGCCGGGTC 
CAGGCCACCT TCCGGAACGC AGATGCGCCG TCCCCACAGG CCTTCGATGA GATGCTCAAG 
AACATTCAGT CCCTCACCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Degradation of FBXO31 by APC/C is regulated by AKT- and ATM-mediated phosphorylation.
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Choppara S,Malonia SK,Sankaran G,Green MR,Santra MK

Structural basis of the phosphorylation-independent recognition of cyclin D1 by the SCF
Proceedings of the National Academy of Sciences of the United States of America115(2)319-324(2018 Jan)
Li Y,Jin K,Bunker E,Zhang X,Luo X,Liu X,Hao B

Overexpression of F-box only protein 31 predicts poor prognosis and deregulates p38?- and JNK-mediated apoptosis in esophageal squamous cell carcinoma.
International journal of cancer142(1)145-155(2018 Jan)
Liu J,Lv L,Gong J,Tan Y,Zhu Y,Dai Y,Pan X,Huen MSY,Li B,Tsao SW,Huo J,Cheung ALM

FBXO31 Suppresses Gastric Cancer EMT by Targeting Snail1 for Proteasomal Degradation.
Molecular cancer research : MCR(2017 Nov)
Zou S,Ma C,Yang F,Xu X,Jia J,Liu Z

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FBXO31 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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