TEFM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TEFM
Entrez Gene ID	79736
Full Name	transcription elongation factor, mitochondrial
Synonyms	C17orf42
General protein information	Preferred Names transcription elongation factor, mitochondrial Names transcription elongation factor, mitochondrial transcription elongation factor of mitochondria
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Disorder MIM:	616422

mRNA and Protein(s)

mRNA	Protein	Name
NM_024683.4	NP_078959.3	transcription elongation factor, mitochondrial
XM_006722084.2	XP_006722147.1	transcription elongation factor, mitochondrial isoform X1
NM_024683.3	NP_078959.3	transcription elongation factor, mitochondrial
XM_006722084.1	XP_006722147.1	transcription elongation factor, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TEFM	NP_078959.3
Pan troglodytes (chimpanzee)	TEFM	XP_511389.2
Danio rerio (zebrafish)	tefm	NP_001073495.1
Canis lupus familiaris (dog)	TEFM	XP_537735.1
Xenopus tropicalis (tropical clawed frog)	tefm	XP_004916371.1
Bos taurus (cattle)	C19H17orf42	NP_001161482.1
Mus musculus (house mouse)	Tefm	NP_899098.2
	LOC713684	XP_001112593.1
Rattus norvegicus (Norway rat)	Tefm	NP_001020797.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism of Transcription Anti-termination in Human Mitochondria.
Hillen HS, Parshin AV, Agaronyan K, Morozov YI, Graber JJ, Chernev A, Schwinghammer K, Urlaub H, Anikin M, Cramer P, Temiakov D
Cell171(5)1082-1093.e13(2017 Nov)

TEFM is a potent stimulator of mitochondrial transcription elongation in vitro.
Posse V, Shahzad S, Falkenberg M, H?llberg BM, Gustafsson CM
Nucleic acids research43(5)2615-24(2015 Mar)

Mitochondrial biology. Replication-transcription switch in human mitochondria.
Agaronyan K, Morozov YI, Anikin M, Temiakov D
Science (New York, N.Y.)347(6221)548-51(2015 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data provide insights into target specificity of TEFM and mechanisms by which it regulates the switch between transcription and replication of mitochondrial DNA.
Title: Mechanism of Transcription Anti-termination in Human Mitochondria.

Data indicate transcription elongation factor (TEFM) as an essential component of the mitochondrial transcription machinery.
Title: TEFM is a potent stimulator of mitochondrial transcription elongation in vitro.

study found interaction of TEFM with mitochondrial RNA polymerase and nascent transcript prevents generation of replication primers and increases transcription processivity and serves as a molecular switch between replication and transcription, which appear to be mutually exclusive processes in mitochondria
Title: Mitochondrial biology. Replication-transcription switch in human mitochondria.

The TEFM protein is proposed to be a critical component of the transcription apparatus of human mitochondria.
Title: TEFM (c17orf42) is necessary for transcription of human mtDNA.

Observational study of gene-disease association. (HuGE Navigator)
Title: RNF135 mutations are not present in patients with Sotos syndrome-like features.

The following TEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13451	NM_024683.4 Latest version!	Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
OHu50668	XM_006722084.2 Latest version!	Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu13451	NM_024683.3	Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
OHu50668	XM_006722084.1	Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TEFM ( NM_024683.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_078959.3 Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.

Price & Availability↑

CloneID	OHu13451
Clone ID Related Accession (Same CDS sequence)	NM_024683.4 , NM_024683.3
Accession Version	NM_024683.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	transcription elongation factor, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA647699.1, AJ314645.1 and BQ003763.1. On Nov 23, 2018 this sequence version replaced NM_024683.3. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1058795.1, SRR5189667.145602.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000581216.6/ ENSP00000462963.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGGGT CTGTCCTCTT CACGGCGGGA GAGAGGTGGA GATGCTTTCT GACCCCGTCG 
AGGTCATCCC TGTACTGGGC CTTACATAAT TTCTGCTGTC GGAAAAAATC CACTACACCT 
AAGAAAATTA CTCCCAATGT TACTTTTTGT GATGAAAATG CAAAGGAGCC CGAAAATGCA 
CTTGACAAGC TCTTCTCTTC AGAACAGCAG GCTTCCATCT TGCATGTGTT GAATACAGCA 
TCTACTAAAG AACTTGAAGC TTTCCGATTG CTTCGTGGAA GAAGGTCCAT CAATATCGTA 
GAGCACAGAG AAAACTTTGG GCCATTTCAG AATTTAGAGA GTTTAATGAA TGTGCCCTTG 
TTTAAGTATA AAAGTACAGT TCAAGTTTGT AACTCCATAC TTTGTCCAAA GACTGGACGG 
GAAAAAAGAA AGTCACCGGA AAACCGGTTC CTGAGAAAGC TCCTCAAACC AGACATAGAA 
AGAGAAAGAC TTAAGGCAGT TAATAGTATC ATATCTATCG TTTTTGGTAC TCGAAGAATT 
GCCTGGGCTC ACCTTGATCG TAAGTTGACA GTGCTGGACT GGCAGCAAAG TGACCGTTGG 
AGTTTAATGA GAGGAATATA CTCATCATCA GTCTATTTAG AAGAGATTTC CTCGATCATT 
TCAAAGATGC CTAAAGCAGA TTTCTATGTT CTGGAAAAAA CAGGACTTTC CATTCAGAAC 
TCATCTCTGT TTCCAATACT GTTACATTTT CATATCATGG AAGCCATGCT GTATGCCTTA 
TTAAATAAAA CTTTTGCCCA GGATGGGCAG CATCAGGTGC TGAGCATGAA TCGAAATGCA 
GTGGGGAAGC ATTTTGAACT GATGATTGGT GACTCCCGGA CTAGTGGAAA AGAGCTAGTG 
AAGCAGTTTC TCTTCGATTC TATACTGAAG GCGGATCCTC GGGTGTTCTT CCCATCAGAT 
AAAATAGTTC ACTACAGACA GATGTTTTTA TCTACTGAAC TACAAAGAGT AGAAGAGCTT 
TATGATTCAT TATTACAAGC TATTGCCTTC TATGAATTAG CAGTGTTTGA CTCTCAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078959.3
CDS	41..1123
Translation	MSGSVLFTAGERWRCFLTPSRSSLYWALHNFCCRKKSTTPKKITPNVTFCDENAKEPENALDKLFSSEQQASILHVLNTASTKELEAFRLLRGRRSINIVEHRENFGPFQNLESLMNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIVFGTRRIAWAHLDRKLTVLDWQQSDRWSLMRGIYSSSVYLEEISSIISKMPKADFYVLEKTGLSIQNSSLFPILLHFHIMEAMLYALLNKTFAQDGQHQVLSMNRNAVGKHFELMIGDSRTSGKELVKQFLFDSILKADPRVFFPSDKIVHYRQMFLSTELQRVEELYDSLLQAIAFYELAVFDSQP

Target ORF information:

RefSeq Version	NM_024683.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024683.4

ORF Insert Sequence:

ATGAGCGGGT CTGTCCTCTT CACGGCGGGA GAGAGGTGGA GATGCTTTCT GACCCCGTCG 
AGGTCATCCC TGTACTGGGC CTTACATAAT TTCTGCTGTC GGAAAAAATC CACTACACCT 
AAGAAAATTA CTCCCAATGT TACTTTTTGT GATGAAAATG CAAAGGAGCC CGAAAATGCA 
CTTGACAAGC TCTTCTCTTC AGAACAGCAG GCTTCCATCT TGCATGTGTT GAATACAGCA 
TCTACTAAAG AACTTGAAGC TTTCCGATTG CTTCGTGGAA GAAGGTCCAT CAATATCGTA 
GAGCACAGAG AAAACTTTGG GCCATTTCAG AATTTAGAGA GTTTAATGAA TGTGCCCTTG 
TTTAAGTATA AAAGTACAGT TCAAGTTTGT AACTCCATAC TTTGTCCAAA GACTGGACGG 
GAAAAAAGAA AGTCACCGGA AAACCGGTTC CTGAGAAAGC TCCTCAAACC AGACATAGAA 
AGAGAAAGAC TTAAGGCAGT TAATAGTATC ATATCTATCG TTTTTGGTAC TCGAAGAATT 
GCCTGGGCTC ACCTTGATCG TAAGTTGACA GTGCTGGACT GGCAGCAAAG TGACCGTTGG 
AGTTTAATGA GAGGAATATA CTCATCATCA GTCTATTTAG AAGAGATTTC CTCGATCATT 
TCAAAGATGC CTAAAGCAGA TTTCTATGTT CTGGAAAAAA CAGGACTTTC CATTCAGAAC 
TCATCTCTGT TTCCAATACT GTTACATTTT CATATCATGG AAGCCATGCT GTATGCCTTA 
TTAAATAAAA CTTTTGCCCA GGATGGGCAG CATCAGGTGC TGAGCATGAA TCGAAATGCA 
GTGGGGAAGC ATTTTGAACT GATGATTGGT GACTCCCGGA CTAGTGGAAA AGAGCTAGTG 
AAGCAGTTTC TCTTCGATTC TATACTGAAG GCGGATCCTC GGGTGTTCTT CCCATCAGAT 
AAAATAGTTC ACTACAGACA GATGTTTTTA TCTACTGAAC TACAAAGAGT AGAAGAGCTT 
TATGATTCAT TATTACAAGC TATTGCCTTC TATGAATTAG CAGTGTTTGA CTCTCAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEFM ( NM_024683.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_078959.3 Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.

Price & Availability↑

CloneID	OHu13451
Clone ID Related Accession (Same CDS sequence)	NM_024683.4 , NM_024683.3
Accession Version	NM_024683.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	transcription elongation factor, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA647699.1, AJ314645.1 and BQ003763.1. On Mar 24, 2007 this sequence version replaced NM_024683.2. ##Evidence-Data-START## Transcript exon combination :: AJ314645.1, SRR1163655.145746.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGGT CTGTCCTCTT CACGGCGGGA GAGAGGTGGA GATGCTTTCT GACCCCGTCG 
AGGTCATCCC TGTACTGGGC CTTACATAAT TTCTGCTGTC GGAAAAAATC CACTACACCT 
AAGAAAATTA CTCCCAATGT TACTTTTTGT GATGAAAATG CAAAGGAGCC CGAAAATGCA 
CTTGACAAGC TCTTCTCTTC AGAACAGCAG GCTTCCATCT TGCATGTGTT GAATACAGCA 
TCTACTAAAG AACTTGAAGC TTTCCGATTG CTTCGTGGAA GAAGGTCCAT CAATATCGTA 
GAGCACAGAG AAAACTTTGG GCCATTTCAG AATTTAGAGA GTTTAATGAA TGTGCCCTTG 
TTTAAGTATA AAAGTACAGT TCAAGTTTGT AACTCCATAC TTTGTCCAAA GACTGGACGG 
GAAAAAAGAA AGTCACCGGA AAACCGGTTC CTGAGAAAGC TCCTCAAACC AGACATAGAA 
AGAGAAAGAC TTAAGGCAGT TAATAGTATC ATATCTATCG TTTTTGGTAC TCGAAGAATT 
GCCTGGGCTC ACCTTGATCG TAAGTTGACA GTGCTGGACT GGCAGCAAAG TGACCGTTGG 
AGTTTAATGA GAGGAATATA CTCATCATCA GTCTATTTAG AAGAGATTTC CTCGATCATT 
TCAAAGATGC CTAAAGCAGA TTTCTATGTT CTGGAAAAAA CAGGACTTTC CATTCAGAAC 
TCATCTCTGT TTCCAATACT GTTACATTTT CATATCATGG AAGCCATGCT GTATGCCTTA 
TTAAATAAAA CTTTTGCCCA GGATGGGCAG CATCAGGTGC TGAGCATGAA TCGAAATGCA 
GTGGGGAAGC ATTTTGAACT GATGATTGGT GACTCCCGGA CTAGTGGAAA AGAGCTAGTG 
AAGCAGTTTC TCTTCGATTC TATACTGAAG GCGGATCCTC GGGTGTTCTT CCCATCAGAT 
AAAATAGTTC ACTACAGACA GATGTTTTTA TCTACTGAAC TACAAAGAGT AGAAGAGCTT 
TATGATTCAT TATTACAAGC TATTGCCTTC TATGAATTAG CAGTGTTTGA CTCTCAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078959.3
CDS	71..1153
Translation	MSGSVLFTAGERWRCFLTPSRSSLYWALHNFCCRKKSTTPKKITPNVTFCDENAKEPENALDKLFSSEQQASILHVLNTASTKELEAFRLLRGRRSINIVEHRENFGPFQNLESLMNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIVFGTRRIAWAHLDRKLTVLDWQQSDRWSLMRGIYSSSVYLEEISSIISKMPKADFYVLEKTGLSIQNSSLFPILLHFHIMEAMLYALLNKTFAQDGQHQVLSMNRNAVGKHFELMIGDSRTSGKELVKQFLFDSILKADPRVFFPSDKIVHYRQMFLSTELQRVEELYDSLLQAIAFYELAVFDSQP

Target ORF information:

RefSeq Version	NM_024683.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription elongation factor, mitochondrial (TEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024683.3

ORF Insert Sequence:

ATGAGCGGGT CTGTCCTCTT CACGGCGGGA GAGAGGTGGA GATGCTTTCT GACCCCGTCG 
AGGTCATCCC TGTACTGGGC CTTACATAAT TTCTGCTGTC GGAAAAAATC CACTACACCT 
AAGAAAATTA CTCCCAATGT TACTTTTTGT GATGAAAATG CAAAGGAGCC CGAAAATGCA 
CTTGACAAGC TCTTCTCTTC AGAACAGCAG GCTTCCATCT TGCATGTGTT GAATACAGCA 
TCTACTAAAG AACTTGAAGC TTTCCGATTG CTTCGTGGAA GAAGGTCCAT CAATATCGTA 
GAGCACAGAG AAAACTTTGG GCCATTTCAG AATTTAGAGA GTTTAATGAA TGTGCCCTTG 
TTTAAGTATA AAAGTACAGT TCAAGTTTGT AACTCCATAC TTTGTCCAAA GACTGGACGG 
GAAAAAAGAA AGTCACCGGA AAACCGGTTC CTGAGAAAGC TCCTCAAACC AGACATAGAA 
AGAGAAAGAC TTAAGGCAGT TAATAGTATC ATATCTATCG TTTTTGGTAC TCGAAGAATT 
GCCTGGGCTC ACCTTGATCG TAAGTTGACA GTGCTGGACT GGCAGCAAAG TGACCGTTGG 
AGTTTAATGA GAGGAATATA CTCATCATCA GTCTATTTAG AAGAGATTTC CTCGATCATT 
TCAAAGATGC CTAAAGCAGA TTTCTATGTT CTGGAAAAAA CAGGACTTTC CATTCAGAAC 
TCATCTCTGT TTCCAATACT GTTACATTTT CATATCATGG AAGCCATGCT GTATGCCTTA 
TTAAATAAAA CTTTTGCCCA GGATGGGCAG CATCAGGTGC TGAGCATGAA TCGAAATGCA 
GTGGGGAAGC ATTTTGAACT GATGATTGGT GACTCCCGGA CTAGTGGAAA AGAGCTAGTG 
AAGCAGTTTC TCTTCGATTC TATACTGAAG GCGGATCCTC GGGTGTTCTT CCCATCAGAT 
AAAATAGTTC ACTACAGACA GATGTTTTTA TCTACTGAAC TACAAAGAGT AGAAGAGCTT 
TATGATTCAT TATTACAAGC TATTGCCTTC TATGAATTAG CAGTGTTTGA CTCTCAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEFM ( XM_006722084.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722147.1 Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50668
Clone ID Related Accession (Same CDS sequence)	XM_006722084.2 , XM_006722084.1
Accession Version	XM_006722084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription elongation factor, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC CCTTGTTTAA GTATAAAAGT ACAGTTCAAG TTTGTAACTC CATACTTTGT 
CCAAAGACTG GACGGGAAAA AAGAAAGTCA CCGGAAAACC GGTTCCTGAG AAAGCTCCTC 
AAACCAGACA TAGAAAGAGA AAGACTTAAG GCAGTTAATA GTATCATATC TATCGTTTTT 
GGTACTCGAA GAATTGCCTG GGCTCACCTT GATCGTAAGT TGACAGTGCT GGACTGGCAG 
CAAAGTGACC GTTGGAGTTT AATGAGAGGA ATATACTCAT CATCAGTCTA TTTAGAAGAG 
ATTTCCTCGA TCATTTCAAA GATGCCTAAA GCAGATTTCT ATGTTCTGGA AAAAACAGGA 
CTTTCCATTC AGAACTCATC TCTGTTTCCA ATACTGTTAC ATTTTCATAT CATGGAAGCC 
ATGCTGTATG CCTTATTAAA TAAAACTTTT GCCCAGGATG GGCAGCATCA GGTGCTGAGC 
ATGAATCGAA ATGCAGTGGG GAAGCATTTT GAACTGATGA TTGGTGACTC CCGGACTAGT 
GGAAAAGAGC TAGTGAAGCA GTTTCTCTTC GATTCTATAC TGAAGGCGGA TCCTCGGGTG 
TTCTTCCCAT CAGATAAAAT AGTTCACTAC AGACAGATGT TTTTATCTAC TGAACTACAA 
AGAGTAGAAG AGCTTTATGA TTCATTATTA CAAGCTATTG CCTTCTATGA ATTAGCAGTG 
TTTGACTCTC AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722147.1
CDS	1754..2491
Translation	MNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIVFGTRRIAWAHLDRKLTVLDWQQSDRWSLMRGIYSSSVYLEEISSIISKMPKADFYVLEKTGLSIQNSSLFPILLHFHIMEAMLYALLNKTFAQDGQHQVLSMNRNAVGKHFELMIGDSRTSGKELVKQFLFDSILKADPRVFFPSDKIVHYRQMFLSTELQRVEELYDSLLQAIAFYELAVFDSQP

Target ORF information:

RefSeq Version	XM_006722084.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722084.2

ORF Insert Sequence:

ATGAATGTGC CCTTGTTTAA GTATAAAAGT ACAGTTCAAG TTTGTAACTC CATACTTTGT 
CCAAAGACTG GACGGGAAAA AAGAAAGTCA CCGGAAAACC GGTTCCTGAG AAAGCTCCTC 
AAACCAGACA TAGAAAGAGA AAGACTTAAG GCAGTTAATA GTATCATATC TATCGTTTTT 
GGTACTCGAA GAATTGCCTG GGCTCACCTT GATCGTAAGT TGACAGTGCT GGACTGGCAG 
CAAAGTGACC GTTGGAGTTT AATGAGAGGA ATATACTCAT CATCAGTCTA TTTAGAAGAG 
ATTTCCTCGA TCATTTCAAA GATGCCTAAA GCAGATTTCT ATGTTCTGGA AAAAACAGGA 
CTTTCCATTC AGAACTCATC TCTGTTTCCA ATACTGTTAC ATTTTCATAT CATGGAAGCC 
ATGCTGTATG CCTTATTAAA TAAAACTTTT GCCCAGGATG GGCAGCATCA GGTGCTGAGC 
ATGAATCGAA ATGCAGTGGG GAAGCATTTT GAACTGATGA TTGGTGACTC CCGGACTAGT 
GGAAAAGAGC TAGTGAAGCA GTTTCTCTTC GATTCTATAC TGAAGGCGGA TCCTCGGGTG 
TTCTTCCCAT CAGATAAAAT AGTTCACTAC AGACAGATGT TTTTATCTAC TGAACTACAA 
AGAGTAGAAG AGCTTTATGA TTCATTATTA CAAGCTATTG CCTTCTATGA ATTAGCAGTG 
TTTGACTCTC AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TEFM ( XM_006722084.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722147.1 Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50668
Clone ID Related Accession (Same CDS sequence)	XM_006722084.2 , XM_006722084.1
Accession Version	XM_006722084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transcription elongation factor, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC CCTTGTTTAA GTATAAAAGT ACAGTTCAAG TTTGTAACTC CATACTTTGT 
CCAAAGACTG GACGGGAAAA AAGAAAGTCA CCGGAAAACC GGTTCCTGAG AAAGCTCCTC 
AAACCAGACA TAGAAAGAGA AAGACTTAAG GCAGTTAATA GTATCATATC TATCGTTTTT 
GGTACTCGAA GAATTGCCTG GGCTCACCTT GATCGTAAGT TGACAGTGCT GGACTGGCAG 
CAAAGTGACC GTTGGAGTTT AATGAGAGGA ATATACTCAT CATCAGTCTA TTTAGAAGAG 
ATTTCCTCGA TCATTTCAAA GATGCCTAAA GCAGATTTCT ATGTTCTGGA AAAAACAGGA 
CTTTCCATTC AGAACTCATC TCTGTTTCCA ATACTGTTAC ATTTTCATAT CATGGAAGCC 
ATGCTGTATG CCTTATTAAA TAAAACTTTT GCCCAGGATG GGCAGCATCA GGTGCTGAGC 
ATGAATCGAA ATGCAGTGGG GAAGCATTTT GAACTGATGA TTGGTGACTC CCGGACTAGT 
GGAAAAGAGC TAGTGAAGCA GTTTCTCTTC GATTCTATAC TGAAGGCGGA TCCTCGGGTG 
TTCTTCCCAT CAGATAAAAT AGTTCACTAC AGACAGATGT TTTTATCTAC TGAACTACAA 
AGAGTAGAAG AGCTTTATGA TTCATTATTA CAAGCTATTG CCTTCTATGA ATTAGCAGTG 
TTTGACTCTC AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722147.1
CDS	900..1637
Translation	MNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIERERLKAVNSIISIVFGTRRIAWAHLDRKLTVLDWQQSDRWSLMRGIYSSSVYLEEISSIISKMPKADFYVLEKTGLSIQNSSLFPILLHFHIMEAMLYALLNKTFAQDGQHQVLSMNRNAVGKHFELMIGDSRTSGKELVKQFLFDSILKADPRVFFPSDKIVHYRQMFLSTELQRVEELYDSLLQAIAFYELAVFDSQP

Target ORF information:

RefSeq Version	XM_006722084.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription elongation factor, mitochondrial (TEFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722084.1

ORF Insert Sequence:

ATGAATGTGC CCTTGTTTAA GTATAAAAGT ACAGTTCAAG TTTGTAACTC CATACTTTGT 
CCAAAGACTG GACGGGAAAA AAGAAAGTCA CCGGAAAACC GGTTCCTGAG AAAGCTCCTC 
AAACCAGACA TAGAAAGAGA AAGACTTAAG GCAGTTAATA GTATCATATC TATCGTTTTT 
GGTACTCGAA GAATTGCCTG GGCTCACCTT GATCGTAAGT TGACAGTGCT GGACTGGCAG 
CAAAGTGACC GTTGGAGTTT AATGAGAGGA ATATACTCAT CATCAGTCTA TTTAGAAGAG 
ATTTCCTCGA TCATTTCAAA GATGCCTAAA GCAGATTTCT ATGTTCTGGA AAAAACAGGA 
CTTTCCATTC AGAACTCATC TCTGTTTCCA ATACTGTTAC ATTTTCATAT CATGGAAGCC 
ATGCTGTATG CCTTATTAAA TAAAACTTTT GCCCAGGATG GGCAGCATCA GGTGCTGAGC 
ATGAATCGAA ATGCAGTGGG GAAGCATTTT GAACTGATGA TTGGTGACTC CCGGACTAGT 
GGAAAAGAGC TAGTGAAGCA GTTTCTCTTC GATTCTATAC TGAAGGCGGA TCCTCGGGTG 
TTCTTCCCAT CAGATAAAAT AGTTCACTAC AGACAGATGT TTTTATCTAC TGAACTACAA 
AGAGTAGAAG AGCTTTATGA TTCATTATTA CAAGCTATTG CCTTCTATGA ATTAGCAGTG 
TTTGACTCTC AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism of Transcription Anti-termination in Human Mitochondria.
Cell171(5)1082-1093.e13(2017 Nov)
Hillen HS,Parshin AV,Agaronyan K,Morozov YI,Graber JJ,Chernev A,Schwinghammer K,Urlaub H,Anikin M,Cramer P,Temiakov D

TEFM is a potent stimulator of mitochondrial transcription elongation in vitro.
Nucleic acids research43(5)2615-24(2015 Mar)
Posse V,Shahzad S,Falkenberg M,H?llberg BM,Gustafsson CM

Mitochondrial biology. Replication-transcription switch in human mitochondria.
Science (New York, N.Y.)347(6221)548-51(2015 Jan)
Agaronyan K,Morozov YI,Anikin M,Temiakov D

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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TEFM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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