gRNAs
The following TBC1D17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu22441 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_024682.2 , NM_024682.3 | |
| Accession Version | NM_024682.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-26 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118341.2, BC003516.1, KC877742.1 and AI927008.1. On Dec 4, 2009 this sequence version replaced NM_024682.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC003516.1, AK022230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_078958.2 |
| CDS | 300..2246 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_024682.2 |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu22441 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_024682.2 , NM_024682.3 | |
| Accession Version | NM_024682.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-01 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC003516.1, KC877742.1 and AI927008.1. On Nov 23, 2018 this sequence version replaced NM_024682.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.925937.1, AK022230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000221543.10/ ENSP00000221543.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_078958.2 |
| CDS | 23..1969 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_024682.3 |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu72070 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011527317.2 , XM_011527317.3 | |
| Accession Version | XM_011527317.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011525619.1 |
| CDS | 212..1717 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011527317.2 |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu72070 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011527317.2 , XM_011527317.3 | |
| Accession Version | XM_011527317.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011525619.1 |
| CDS | 194..1699 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011527317.3 |
1 | ATGGAAGGAG CCGGCTACAG GGTGGTGTTT GAGAAGGGCG GAGTGTACCT GCACACCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12929 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001168222.2 , NM_001168222.1 | |
| Accession Version | NM_001168222.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-30 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK300007.1, KC877742.1 and AI805386.1. On May 31, 2019 this sequence version replaced NM_001168222.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK300007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAAGGAG CCGGCTACAG GGACAATGAC GTCCTCCTGC ACTGGGCTCC TGTAGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001161694.1 |
| CDS | 23..1870 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001168222.2 |
1 | ATGGAAGGAG CCGGCTACAG GGACAATGAC GTCCTCCTGC ACTGGGCTCC TGTAGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12929 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001168222.2 , NM_001168222.1 | |
| Accession Version | NM_001168222.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-23 | |
| Organism | Homo sapiens(human) | |
| Product | TBC1 domain family member 17 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118341.2, AK300007.1, KC877742.1 and AI805386.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK300007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAAGGAG CCGGCTACAG GGACAATGAC GTCCTCCTGC ACTGGGCTCC TGTAGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001161694.1 |
| CDS | 300..2147 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001168222.1 |
1 | ATGGAAGGAG CCGGCTACAG GGACAATGAC GTCCTCCTGC ACTGGGCTCC TGTAGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy. |
Optineurin mediates a negative regulation of Rab8 by the GTPase-activating protein TBC1D17. |
Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. |
Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. |
Functional dissection of Rab GTPases involved in primary cilium formation. |
