CHPF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHPF
Entrez Gene ID	79586
Full Name	chondroitin polymerizing factor
Synonyms	CHSY2,CSS2
General protein information	Preferred Names chondroitin polymerizing factor Names chondroitin sulfate synthase 2 N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II N-acetylgalactosaminyltransferase 2 chondroitin glucuronyltransferase 2 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Disorder MIM:	610405

mRNA and Protein(s)

mRNA	Protein	Name
XM_011511838.2	XP_011510140.1	chondroitin sulfate synthase 2 isoform X1
NM_024536.6	NP_078812.3	chondroitin sulfate synthase 2 isoform 1
NM_001195731.1	NP_001182660.1	chondroitin sulfate synthase 2 isoform 2
XM_011511838.3	XP_011510140.1	chondroitin sulfate synthase 2 isoform X1
NM_024536.5	NP_078812.2	chondroitin sulfate synthase 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chpf	NP_001005906.1
Danio rerio (zebrafish)	chpfa	XP_002663375.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001050	XP_318249.4
Canis lupus familiaris (dog)	CHPF	XP_003640243.1
Drosophila melanogaster (fruit fly)	CG43313	NP_572853.2
Bos taurus (cattle)	CHPF	NP_001192680.1
Mus musculus (house mouse)	Chpf	NP_001001566.1
Danio rerio (zebrafish)	LOC794660	XP_001923418.1
Caenorhabditis elegans (roundworm)	mig-22	NP_498934.1
Homo sapiens (human)	CHPF	NP_078812.2
Pan troglodytes (chimpanzee)	CHPF	XP_003309517.1
	CHPF	XP_001104808.1
Gallus gallus (chicken)	CHPF	XP_001232317.2
Xenopus tropicalis (tropical clawed frog)	chpf	XP_002942593.2

Gene Ontology
Bibliography

Related articles in PubMed

TGF-? Stimulates Expression of Chondroitin Polymerizing Factor in Nucleus Pulposus Cells Through the Smad3, RhoA/ROCK1, and MAPK Signaling Pathways.
Hu B, Xu C, Cao P, Tian Y, Zhang Y, Shi C, Xu J, Yuan W, Chen H
Journal of cellular biochemistry119(1)566-579(2018 Jan)

Lentivirus?mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in?vitro.
Fan YH, Xiao B, Lv SG, Ye MH, Zhu XG, Wu MJ
Oncology reports38(2)1149-1155(2017 Aug)

Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential.
Kuroda Y, Sako W, Goto S, Sawada T, Uchida D, Izumi Y, Takahashi T, Kagawa N, Matsumoto M, Matsumoto M, Takahashi R, Kaji R, Mitsui T
Human molecular genetics21(5)991-1003(2012 Mar)

FOXM1 induces a global methylation signature that mimics the cancer epigenome in head and neck squamous cell carcinoma.
Teh MT, Gemenetzidis E, Patel D, Tariq R, Nadir A, Bahta AW, Waseem A, Hutchison IL
PloS one7(3)e34329(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study reports a novel protein named Klokin 1(a splice variant of chondroitin polymerizing factor) that transports Parkin to the mitochondria; findings indicate that mitochondrial Parkin prevents mitochondrial depolarization and that Klokin 1 may compensate for Parkin deficiency
Title: Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential.

CHPF was found to be differentially methylated in human squamous cell carcinomas.
Title: FOXM1 induces a global methylation signature that mimics the cancer epigenome in head and neck squamous cell carcinoma.

The present study focused on the expression of chondroitin-synthesizing enzymes in colorectal cancer.
Title: Chondroitin synthases I, II, III and chondroitin sulfate glucuronyltransferase expression in colorectal cancer.

Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function
Title: Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function.

chondroitin polymerizing activity requires concomitant expression of a ChPF with ChSy; coexpression of the ChPF and ChSy yielded markedly augmented glycosyltransferase activities, whereas simple mixing of the two separately expressed proteins did not.
Title: Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

The following CHPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32328	NM_001195731.1 Latest version!	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu71900	XM_011511838.3 Latest version!	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu04098	NM_024536.5 Latest version!	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu107333	NM_024536.6 Latest version!	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu71900	XM_011511838.2	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHPF ( NM_001195731.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182660.1 Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32328
Clone ID Related Accession (Same CDS sequence)	NM_001195731.1
Accession Version	NM_001195731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK302124.1, DC341343.1, AB086063.1 and BC021223.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1, resulting in translation initiation from an in-frame downstream AUG, and an isoform (2) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302124.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGG TGACGCTAGG CGAGGAGCGA CCCATTGGAC ACCTGCACCT GGCGCTGCGC 
CACCTGCTGG AGCAGCACGG CGACGACTTT GACTGGTTCT TCCTGGTGCC TGACACCACC 
TACACCGAGG CGCACGGCCT GGCACGCCTA ACTGGCCACC TCAGCCTGGC CTCCGCCGCC 
CACCTGTACC TGGGCCGGCC CCAGGACTTC ATCGGCGGAG AGCCCACCCC CGGCCGCTAC 
TGCCACGGAG GCTTTGGGGT GCTGCTGTCG CGCATGCTGC TGCAACAACT GCGCCCCCAC 
CTGGAAGGCT GCCGCAACGA CATCGTCAGT GCGCGCCCTG ACGAGTGGCT GGGTCGCTGC 
ATTCTCGATG CCACCGGGGT GGGCTGCACT GGTGACCACG AGGGGGTGCA CTATAGCCAT 
CTGGAGCTGA GCCCTGGGGA GCCAGTGCAG GAGGGGGACC CTCATTTCCG AAGTGCCCTG 
ACAGCCCACC CTGTGCGTGA CCCTGTGCAC ATGTACCAGC TGCACAAAGC TTTCGCCCGA 
GCTGAACTGG AACGCACGTA CCAGGAGATC CAGGAGTTAC AGTGGGAGAT CCAGAATACC 
AGCCATCTGG CCGTTGATGG GGACCGGGCA GCTGCTTGGC CCGTGGGTAT TCCAGCACCA 
TCCCGCCCGG CCTCCCGCTT TGAGGTGCTG CGCTGGGACT ACTTCACGGA GCAGCACGCT 
TTCTCCTGCG CCGATGGCTC ACCCCGCTGC CCACTGCGTG GGGCTGACCG GGCTGATGTG 
GCCGATGTTC TGGGGACAGC TCTAGAGGAG CTGAACCGCC GCTACCACCC GGCCTTGCGG 
CTCCAGAAGC AGCAGCTGGT GAATGGCTAC CGACGCTTTG ATCCGGCCCG GGGTATGGAA 
TACACGCTGG ACTTGCAGCT GGAGGCACTG ACCCCCCAGG GAGGCCGCCG GCCCCTCACT 
CGCCGAGTGC AGCTGCTCCG GCCGCTGAGC CGCGTGGAGA TCTTGCCTGT GCCCTATGTC 
ACTGAGGCCT CACGTCTCAC TGTGCTGCTG CCTCTAGCTG CGGCTGAGCG TGACCTGGCC 
CCTGGCTTCT TGGAGGCCTT TGCCACTGCA GCACTGGAGC CTGGTGATGC TGCGGCAGCC 
CTGACCCTGC TGCTACTGTA TGAGCCGCGC CAGGCCCAGC GCGTGGCCCA TGCAGATGTC 
TTCGCACCTG TCAAGGCCCA CGTGGCAGAG CTGGAGCGGC GTTTCCCCGG TGCCCGGGTG 
CCATGGCTCA GTGTGCAGAC AGCCGCACCC TCACCACTGC GCCTCATGGA TCTACTCTCC 
AAGAAGCACC CGCTGGACAC ACTGTTCCTG CTGGCCGGGC CAGACACGGT GCTCACGCCT 
GACTTCCTGA ACCGCTGCCG CATGCATGCC ATCTCCGGCT GGCAGGCCTT CTTTCCCATG 
CATTTCCAAG CCTTCCACCC AGCTGTGGCC CCACCACAAG GGCCTGGGCC CCCAGAGCTG 
GGCCGTGACA CTGGCCGCTT TGATCGCCAG GCAGCCAGCG AGGCCTGCTT CTACAACTCC 
GACTACGTGG CAGCCCGTGG GCGCCTGGCG GCAGCCTCAG AACAAGAAGA GGAGCTGCTG 
GAGAGCCTGG ATGTGTACGA GCTGTTCCTC CACTTCTCCA GTCTGCATGT GCTGCGGGCG 
GTGGAGCCGG CGCTGCTGCA GCGCTACCGG GCCCAGACGT GCAGCGCGAG GCTCAGTGAG 
GACCTGTACC ACCGCTGCCT CCAGAGCGTG CTTGAGGGCC TCGGCTCCCG AACCCAGCTG 
GCCATGCTAC TCTTTGAACA GGAGCAGGGC AACAGCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182660.1
CDS	358..2199
Translation	MAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSLASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDRAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	NM_001195731.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195731.1

ORF Insert Sequence:

ATGGCAGTGG TGACGCTAGG CGAGGAGCGA CCCATTGGAC ACCTGCACCT GGCGCTGCGC 
CACCTGCTGG AGCAGCACGG CGACGACTTT GACTGGTTCT TCCTGGTGCC TGACACCACC 
TACACCGAGG CGCACGGCCT GGCACGCCTA ACTGGCCACC TCAGCCTGGC CTCCGCCGCC 
CACCTGTACC TGGGCCGGCC CCAGGACTTC ATCGGCGGAG AGCCCACCCC CGGCCGCTAC 
TGCCACGGAG GCTTTGGGGT GCTGCTGTCG CGCATGCTGC TGCAACAACT GCGCCCCCAC 
CTGGAAGGCT GCCGCAACGA CATCGTCAGT GCGCGCCCTG ACGAGTGGCT GGGTCGCTGC 
ATTCTCGATG CCACCGGGGT GGGCTGCACT GGTGACCACG AGGGGGTGCA CTATAGCCAT 
CTGGAGCTGA GCCCTGGGGA GCCAGTGCAG GAGGGGGACC CTCATTTCCG AAGTGCCCTG 
ACAGCCCACC CTGTGCGTGA CCCTGTGCAC ATGTACCAGC TGCACAAAGC TTTCGCCCGA 
GCTGAACTGG AACGCACGTA CCAGGAGATC CAGGAGTTAC AGTGGGAGAT CCAGAATACC 
AGCCATCTGG CCGTTGATGG GGACCGGGCA GCTGCTTGGC CCGTGGGTAT TCCAGCACCA 
TCCCGCCCGG CCTCCCGCTT TGAGGTGCTG CGCTGGGACT ACTTCACGGA GCAGCACGCT 
TTCTCCTGCG CCGATGGCTC ACCCCGCTGC CCACTGCGTG GGGCTGACCG GGCTGATGTG 
GCCGATGTTC TGGGGACAGC TCTAGAGGAG CTGAACCGCC GCTACCACCC GGCCTTGCGG 
CTCCAGAAGC AGCAGCTGGT GAATGGCTAC CGACGCTTTG ATCCGGCCCG GGGTATGGAA 
TACACGCTGG ACTTGCAGCT GGAGGCACTG ACCCCCCAGG GAGGCCGCCG GCCCCTCACT 
CGCCGAGTGC AGCTGCTCCG GCCGCTGAGC CGCGTGGAGA TCTTGCCTGT GCCCTATGTC 
ACTGAGGCCT CACGTCTCAC TGTGCTGCTG CCTCTAGCTG CGGCTGAGCG TGACCTGGCC 
CCTGGCTTCT TGGAGGCCTT TGCCACTGCA GCACTGGAGC CTGGTGATGC TGCGGCAGCC 
CTGACCCTGC TGCTACTGTA TGAGCCGCGC CAGGCCCAGC GCGTGGCCCA TGCAGATGTC 
TTCGCACCTG TCAAGGCCCA CGTGGCAGAG CTGGAGCGGC GTTTCCCCGG TGCCCGGGTG 
CCATGGCTCA GTGTGCAGAC AGCCGCACCC TCACCACTGC GCCTCATGGA TCTACTCTCC 
AAGAAGCACC CGCTGGACAC ACTGTTCCTG CTGGCCGGGC CAGACACGGT GCTCACGCCT 
GACTTCCTGA ACCGCTGCCG CATGCATGCC ATCTCCGGCT GGCAGGCCTT CTTTCCCATG 
CATTTCCAAG CCTTCCACCC AGCTGTGGCC CCACCACAAG GGCCTGGGCC CCCAGAGCTG 
GGCCGTGACA CTGGCCGCTT TGATCGCCAG GCAGCCAGCG AGGCCTGCTT CTACAACTCC 
GACTACGTGG CAGCCCGTGG GCGCCTGGCG GCAGCCTCAG AACAAGAAGA GGAGCTGCTG 
GAGAGCCTGG ATGTGTACGA GCTGTTCCTC CACTTCTCCA GTCTGCATGT GCTGCGGGCG 
GTGGAGCCGG CGCTGCTGCA GCGCTACCGG GCCCAGACGT GCAGCGCGAG GCTCAGTGAG 
GACCTGTACC ACCGCTGCCT CCAGAGCGTG CTTGAGGGCC TCGGCTCCCG AACCCAGCTG 
GCCATGCTAC TCTTTGAACA GGAGCAGGGC AACAGCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHPF ( XM_011511838.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510140.1 Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71900
Clone ID Related Accession (Same CDS sequence)	XM_011511838.2 , XM_011511838.3
Accession Version	XM_011511838.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGA TACAGGGGGT GCACTATAGC CATCTGGAGC TGAGCCCTGG GGAGCCAGTG 
CAGGAGGGGG ACCCTCATTT CCGAAGTGCC CTGACAGCCC ACCCTGTGCG TGACCCTGTG 
CACATGTACC AGCTGCACAA AGCTTTCGCC CGAGCTGAAC TGGAACGCAC GTACCAGGAG 
ATCCAGGAGT TACAGTGGGA GATCCAGAAT ACCAGCCATC TGGCCGTTGA TGGGGACCAG 
GCAGCTGCTT GGCCCGTGGG TATTCCAGCA CCATCCCGCC CGGCCTCCCG CTTTGAGGTG 
CTGCGCTGGG ACTACTTCAC GGAGCAGCAC GCTTTCTCCT GCGCCGATGG CTCACCCCGC 
TGCCCACTGC GTGGGGCTGA CCGGGCTGAT GTGGCCGATG TTCTGGGGAC AGCTCTAGAG 
GAGCTGAACC GCCGCTACCA CCCGGCCTTG CGGCTCCAGA AGCAGCAGCT GGTGAATGGC 
TACCGACGCT TTGATCCGGC CCGGGGTATG GAATACACGC TGGACTTGCA GCTGGAGGCA 
CTGACCCCCC AGGGAGGCCG CCGGCCCCTC ACTCGCCGAG TGCAGCTGCT CCGGCCGCTG 
AGCCGCGTGG AGATCTTGCC TGTGCCCTAT GTCACTGAGG CCTCACGTCT CACTGTGCTG 
CTGCCTCTAG CTGCGGCTGA GCGTGACCTG GCCCCTGGCT TCTTGGAGGC CTTTGCCACT 
GCAGCACTGG AGCCTGGTGA TGCTGCGGCA GCCCTGACCC TGCTGCTACT GTATGAGCCG 
CGCCAGGCCC AGCGCGTGGC CCATGCAGAT GTCTTCGCAC CTGTCAAGGC CCACGTGGCA 
GAGCTGGAGC GGCGTTTCCC CGGTGCCCGG GTGCCATGGC TCAGTGTGCA GACAGCCGCA 
CCCTCACCAC TGCGCCTCAT GGATCTACTC TCCAAGAAGC ACCCGCTGGA CACACTGTTC 
CTGCTGGCCG GGCCAGACAC GGTGCTCACG CCTGACTTCC TGAACCGCTG CCGCATGCAT 
GCCATCTCCG GCTGGCAGGC CTTCTTTCCC ATGCATTTCC AAGCCTTCCA CCCAGCTGTG 
GCCCCACCAC AAGGGCCTGG GCCCCCAGAG CTGGGCCGTG ACACTGGCCG CTTTGATCGC 
CAGGCAGCCA GCGAGGCCTG CTTCTACAAC TCCGACTATG TGGCAGCCCG TGGGCGCCTG 
GCGGCAGCCT CAGAACAAGA AGAGGAGCTG CTGGAGAGCC TGGATGTGTA CGAGCTGTTC 
CTCCACTTCT CCAGTCTGCA TGTGCTGCGG GCGGTGGAGC CGGCGCTGCT GCAGCGCTAC 
CGGGCCCAGA CGTGCAGCGC GAGGCTCAGT GAGGACCTGT ACCACCGCTG CCTCCAGAGC 
GTGCTTGAGG GCCTCGGCTC CCGAACCCAG CTGGCCATGC TACTCTTTGA ACAGGAGCAG 
GGCAACAGCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510140.1
CDS	632..2086
Translation	MLEIQGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	XM_011511838.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511838.2

ORF Insert Sequence:

ATGTTGGAGA TACAGGGGGT GCACTATAGC CATCTGGAGC TGAGCCCTGG GGAGCCAGTG 
CAGGAGGGGG ACCCTCATTT CCGAAGTGCC CTGACAGCCC ACCCTGTGCG TGACCCTGTG 
CACATGTACC AGCTGCACAA AGCTTTCGCC CGAGCTGAAC TGGAACGCAC GTACCAGGAG 
ATCCAGGAGT TACAGTGGGA GATCCAGAAT ACCAGCCATC TGGCCGTTGA TGGGGACCAG 
GCAGCTGCTT GGCCCGTGGG TATTCCAGCA CCATCCCGCC CGGCCTCCCG CTTTGAGGTG 
CTGCGCTGGG ACTACTTCAC GGAGCAGCAC GCTTTCTCCT GCGCCGATGG CTCACCCCGC 
TGCCCACTGC GTGGGGCTGA CCGGGCTGAT GTGGCCGATG TTCTGGGGAC AGCTCTAGAG 
GAGCTGAACC GCCGCTACCA CCCGGCCTTG CGGCTCCAGA AGCAGCAGCT GGTGAATGGC 
TACCGACGCT TTGATCCGGC CCGGGGTATG GAATACACGC TGGACTTGCA GCTGGAGGCA 
CTGACCCCCC AGGGAGGCCG CCGGCCCCTC ACTCGCCGAG TGCAGCTGCT CCGGCCGCTG 
AGCCGCGTGG AGATCTTGCC TGTGCCCTAT GTCACTGAGG CCTCACGTCT CACTGTGCTG 
CTGCCTCTAG CTGCGGCTGA GCGTGACCTG GCCCCTGGCT TCTTGGAGGC CTTTGCCACT 
GCAGCACTGG AGCCTGGTGA TGCTGCGGCA GCCCTGACCC TGCTGCTACT GTATGAGCCG 
CGCCAGGCCC AGCGCGTGGC CCATGCAGAT GTCTTCGCAC CTGTCAAGGC CCACGTGGCA 
GAGCTGGAGC GGCGTTTCCC CGGTGCCCGG GTGCCATGGC TCAGTGTGCA GACAGCCGCA 
CCCTCACCAC TGCGCCTCAT GGATCTACTC TCCAAGAAGC ACCCGCTGGA CACACTGTTC 
CTGCTGGCCG GGCCAGACAC GGTGCTCACG CCTGACTTCC TGAACCGCTG CCGCATGCAT 
GCCATCTCCG GCTGGCAGGC CTTCTTTCCC ATGCATTTCC AAGCCTTCCA CCCAGCTGTG 
GCCCCACCAC AAGGGCCTGG GCCCCCAGAG CTGGGCCGTG ACACTGGCCG CTTTGATCGC 
CAGGCAGCCA GCGAGGCCTG CTTCTACAAC TCCGACTATG TGGCAGCCCG TGGGCGCCTG 
GCGGCAGCCT CAGAACAAGA AGAGGAGCTG CTGGAGAGCC TGGATGTGTA CGAGCTGTTC 
CTCCACTTCT CCAGTCTGCA TGTGCTGCGG GCGGTGGAGC CGGCGCTGCT GCAGCGCTAC 
CGGGCCCAGA CGTGCAGCGC GAGGCTCAGT GAGGACCTGT ACCACCGCTG CCTCCAGAGC 
GTGCTTGAGG GCCTCGGCTC CCGAACCCAG CTGGCCATGC TACTCTTTGA ACAGGAGCAG 
GGCAACAGCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHPF ( XM_011511838.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510140.1 Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71900
Clone ID Related Accession (Same CDS sequence)	XM_011511838.2 , XM_011511838.3
Accession Version	XM_011511838.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511838.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGA TACAGGGGGT GCACTATAGC CATCTGGAGC TGAGCCCTGG GGAGCCAGTG 
CAGGAGGGGG ACCCTCATTT CCGAAGTGCC CTGACAGCCC ACCCTGTGCG TGACCCTGTG 
CACATGTACC AGCTGCACAA AGCTTTCGCC CGAGCTGAAC TGGAACGCAC GTACCAGGAG 
ATCCAGGAGT TACAGTGGGA GATCCAGAAT ACCAGCCATC TGGCCGTTGA TGGGGACCAG 
GCAGCTGCTT GGCCCGTGGG TATTCCAGCA CCATCCCGCC CGGCCTCCCG CTTTGAGGTG 
CTGCGCTGGG ACTACTTCAC GGAGCAGCAC GCTTTCTCCT GCGCCGATGG CTCACCCCGC 
TGCCCACTGC GTGGGGCTGA CCGGGCTGAT GTGGCCGATG TTCTGGGGAC AGCTCTAGAG 
GAGCTGAACC GCCGCTACCA CCCGGCCTTG CGGCTCCAGA AGCAGCAGCT GGTGAATGGC 
TACCGACGCT TTGATCCGGC CCGGGGTATG GAATACACGC TGGACTTGCA GCTGGAGGCA 
CTGACCCCCC AGGGAGGCCG CCGGCCCCTC ACTCGCCGAG TGCAGCTGCT CCGGCCGCTG 
AGCCGCGTGG AGATCTTGCC TGTGCCCTAT GTCACTGAGG CCTCACGTCT CACTGTGCTG 
CTGCCTCTAG CTGCGGCTGA GCGTGACCTG GCCCCTGGCT TCTTGGAGGC CTTTGCCACT 
GCAGCACTGG AGCCTGGTGA TGCTGCGGCA GCCCTGACCC TGCTGCTACT GTATGAGCCG 
CGCCAGGCCC AGCGCGTGGC CCATGCAGAT GTCTTCGCAC CTGTCAAGGC CCACGTGGCA 
GAGCTGGAGC GGCGTTTCCC CGGTGCCCGG GTGCCATGGC TCAGTGTGCA GACAGCCGCA 
CCCTCACCAC TGCGCCTCAT GGATCTACTC TCCAAGAAGC ACCCGCTGGA CACACTGTTC 
CTGCTGGCCG GGCCAGACAC GGTGCTCACG CCTGACTTCC TGAACCGCTG CCGCATGCAT 
GCCATCTCCG GCTGGCAGGC CTTCTTTCCC ATGCATTTCC AAGCCTTCCA CCCAGCTGTG 
GCCCCACCAC AAGGGCCTGG GCCCCCAGAG CTGGGCCGTG ACACTGGCCG CTTTGATCGC 
CAGGCAGCCA GCGAGGCCTG CTTCTACAAC TCCGACTATG TGGCAGCCCG TGGGCGCCTG 
GCGGCAGCCT CAGAACAAGA AGAGGAGCTG CTGGAGAGCC TGGATGTGTA CGAGCTGTTC 
CTCCACTTCT CCAGTCTGCA TGTGCTGCGG GCGGTGGAGC CGGCGCTGCT GCAGCGCTAC 
CGGGCCCAGA CGTGCAGCGC GAGGCTCAGT GAGGACCTGT ACCACCGCTG CCTCCAGAGC 
GTGCTTGAGG GCCTCGGCTC CCGAACCCAG CTGGCCATGC TACTCTTTGA ACAGGAGCAG 
GGCAACAGCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510140.1
CDS	694..2148
Translation	MLEIQGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	XM_011511838.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511838.3

ORF Insert Sequence:

ATGTTGGAGA TACAGGGGGT GCACTATAGC CATCTGGAGC TGAGCCCTGG GGAGCCAGTG 
CAGGAGGGGG ACCCTCATTT CCGAAGTGCC CTGACAGCCC ACCCTGTGCG TGACCCTGTG 
CACATGTACC AGCTGCACAA AGCTTTCGCC CGAGCTGAAC TGGAACGCAC GTACCAGGAG 
ATCCAGGAGT TACAGTGGGA GATCCAGAAT ACCAGCCATC TGGCCGTTGA TGGGGACCAG 
GCAGCTGCTT GGCCCGTGGG TATTCCAGCA CCATCCCGCC CGGCCTCCCG CTTTGAGGTG 
CTGCGCTGGG ACTACTTCAC GGAGCAGCAC GCTTTCTCCT GCGCCGATGG CTCACCCCGC 
TGCCCACTGC GTGGGGCTGA CCGGGCTGAT GTGGCCGATG TTCTGGGGAC AGCTCTAGAG 
GAGCTGAACC GCCGCTACCA CCCGGCCTTG CGGCTCCAGA AGCAGCAGCT GGTGAATGGC 
TACCGACGCT TTGATCCGGC CCGGGGTATG GAATACACGC TGGACTTGCA GCTGGAGGCA 
CTGACCCCCC AGGGAGGCCG CCGGCCCCTC ACTCGCCGAG TGCAGCTGCT CCGGCCGCTG 
AGCCGCGTGG AGATCTTGCC TGTGCCCTAT GTCACTGAGG CCTCACGTCT CACTGTGCTG 
CTGCCTCTAG CTGCGGCTGA GCGTGACCTG GCCCCTGGCT TCTTGGAGGC CTTTGCCACT 
GCAGCACTGG AGCCTGGTGA TGCTGCGGCA GCCCTGACCC TGCTGCTACT GTATGAGCCG 
CGCCAGGCCC AGCGCGTGGC CCATGCAGAT GTCTTCGCAC CTGTCAAGGC CCACGTGGCA 
GAGCTGGAGC GGCGTTTCCC CGGTGCCCGG GTGCCATGGC TCAGTGTGCA GACAGCCGCA 
CCCTCACCAC TGCGCCTCAT GGATCTACTC TCCAAGAAGC ACCCGCTGGA CACACTGTTC 
CTGCTGGCCG GGCCAGACAC GGTGCTCACG CCTGACTTCC TGAACCGCTG CCGCATGCAT 
GCCATCTCCG GCTGGCAGGC CTTCTTTCCC ATGCATTTCC AAGCCTTCCA CCCAGCTGTG 
GCCCCACCAC AAGGGCCTGG GCCCCCAGAG CTGGGCCGTG ACACTGGCCG CTTTGATCGC 
CAGGCAGCCA GCGAGGCCTG CTTCTACAAC TCCGACTATG TGGCAGCCCG TGGGCGCCTG 
GCGGCAGCCT CAGAACAAGA AGAGGAGCTG CTGGAGAGCC TGGATGTGTA CGAGCTGTTC 
CTCCACTTCT CCAGTCTGCA TGTGCTGCGG GCGGTGGAGC CGGCGCTGCT GCAGCGCTAC 
CGGGCCCAGA CGTGCAGCGC GAGGCTCAGT GAGGACCTGT ACCACCGCTG CCTCCAGAGC 
GTGCTTGAGG GCCTCGGCTC CCGAACCCAG CTGGCCATGC TACTCTTTGA ACAGGAGCAG 
GGCAACAGCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHPF ( NM_024536.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_078812.2 Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04098
Clone ID Related Accession (Same CDS sequence)	NM_024536.5
Accession Version	NM_024536.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY358403.1, AB086063.1 and BC021223.2. On Feb 21, 2008 this sequence version replaced NM_024536.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC021223.2, AB095813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCAT CGCTGCTGCT GTCGGTGCTG CGGCCCGCAG GGCCCGTGGC CGTGGGCATC 
TCCCTGGGCT TCACCCTGAG CCTGCTCAGC GTCACCTGGG TGGAGGAGCC GTGCGGCCCA 
GGCCCGCCCC AACCTGGAGA CTCTGAGCTG CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAAGC CCGGGGCCGG CGAAGGCGCC 
GGGGAGAATT GGGAGCCGCG CGTCTTGCCC TACCACCCTG CACAGCCCGG CCAGGCCGCC 
AAAAAGGCCG TCAGGACCCG CTACATCAGC ACGGAGCTGG GCATCAGGCA GAGGCTGCTG 
GTGGCGGTGC TGACCTCTCA GACCACGCTG CCCACGCTGG GCGTGGCCGT GAACCGCACG 
CTGGGGCACC GGCTGGAGCG TGTGGTGTTC CTGACGGGCG CACGGGGCCG CCGGGCCCCA 
CCTGGCATGG CAGTGGTGAC GCTAGGCGAG GAGCGACCCA TTGGACACCT GCACCTGGCG 
CTGCGCCACC TGCTGGAGCA GCACGGCGAC GACTTTGACT GGTTCTTCCT GGTGCCTGAC 
ACCACCTACA CCGAGGCGCA CGGCCTGGCA CGCCTAACTG GCCACCTCAG CCTGGCCTCC 
GCCGCCCACC TGTACCTGGG CCGGCCCCAG GACTTCATCG GCGGAGAGCC CACCCCCGGC 
CGCTACTGCC ACGGAGGCTT TGGGGTGCTG CTGTCGCGCA TGCTGCTGCA ACAACTGCGC 
CCCCACCTGG AAGGCTGCCG CAACGACATC GTCAGTGCGC GCCCTGACGA GTGGCTGGGT 
CGCTGCATTC TCGATGCCAC CGGGGTGGGC TGCACTGGTG ACCACGAGGG GGTGCACTAT 
AGCCATCTGG AGCTGAGCCC TGGGGAGCCA GTGCAGGAGG GGGACCCTCA TTTCCGAAGT 
GCCCTGACAG CCCACCCTGT GCGTGACCCT GTGCACATGT ACCAGCTGCA CAAAGCTTTC 
GCCCGAGCTG AACTGGAACG CACGTACCAG GAGATCCAGG AGTTACAGTG GGAGATCCAG 
AATACCAGCC ATCTGGCCGT TGATGGGGAC CGGGCAGCTG CTTGGCCCGT GGGTATTCCA 
GCACCATCCC GCCCGGCCTC CCGCTTTGAG GTGCTGCGCT GGGACTACTT CACGGAGCAG 
CACGCTTTCT CCTGCGCCGA TGGCTCACCC CGCTGCCCAC TGCGTGGGGC TGACCGGGCT 
GATGTGGCCG ATGTTCTGGG GACAGCTCTA GAGGAGCTGA ACCGCCGCTA CCACCCGGCC 
TTGCGGCTCC AGAAGCAGCA GCTGGTGAAT GGCTACCGAC GCTTTGATCC GGCCCGGGGT 
ATGGAATACA CGCTGGACTT GCAGCTGGAG GCACTGACCC CCCAGGGAGG CCGCCGGCCC 
CTCACTCGCC GAGTGCAGCT GCTCCGGCCG CTGAGCCGCG TGGAGATCTT GCCTGTGCCC 
TATGTCACTG AGGCCTCACG TCTCACTGTG CTGCTGCCTC TAGCTGCGGC TGAGCGTGAC 
CTGGCCCCTG GCTTCTTGGA GGCCTTTGCC ACTGCAGCAC TGGAGCCTGG TGATGCTGCG 
GCAGCCCTGA CCCTGCTGCT ACTGTATGAG CCGCGCCAGG CCCAGCGCGT GGCCCATGCA 
GATGTCTTCG CACCTGTCAA GGCCCACGTG GCAGAGCTGG AGCGGCGTTT CCCCGGTGCC 
CGGGTGCCAT GGCTCAGTGT GCAGACAGCC GCACCCTCAC CACTGCGCCT CATGGATCTA 
CTCTCCAAGA AGCACCCGCT GGACACACTG TTCCTGCTGG CCGGGCCAGA CACGGTGCTC 
ACGCCTGACT TCCTGAACCG CTGCCGCATG CATGCCATCT CCGGCTGGCA GGCCTTCTTT 
CCCATGCATT TCCAAGCCTT CCACCCAGCT GTGGCCCCAC CACAAGGGCC TGGGCCCCCA 
GAGCTGGGCC GTGACACTGG CCGCTTTGAT CGCCAGGCAG CCAGCGAGGC CTGCTTCTAC 
AACTCCGACT ACGTGGCAGC CCGTGGGCGC CTGGCGGCAG CCTCAGAACA AGAAGAGGAG 
CTGCTGGAGA GCCTGGATGT GTACGAGCTG TTCCTCCACT TCTCCAGTCT GCATGTGCTG 
CGGGCGGTGG AGCCGGCGCT GCTGCAGCGC TACCGGGCCC AGACGTGCAG CGCGAGGCTC 
AGTGAGGACC TGTACCACCG CTGCCTCCAG AGCGTGCTTG AGGGCCTCGG CTCCCGAACC 
CAGCTGGCCA TGCTACTCTT TGAACAGGAG CAGGGCAACA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078812.2
CDS	228..2555
Translation	MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGAEREKPGAGEGAGENWEPRVLPYHPAQPGQAAKKAVRTRYISTELGIRQRLLVAVLTSQTTLPTLGVAVNRTLGHRLERVVFLTGARGRRAPPGMAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSLASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDRAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	NM_024536.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024536.5

ORF Insert Sequence:

ATGCGGGCAT CGCTGCTGCT GTCGGTGCTG CGGCCCGCAG GGCCCGTGGC CGTGGGCATC 
TCCCTGGGCT TCACCCTGAG CCTGCTCAGC GTCACCTGGG TGGAGGAGCC GTGCGGCCCA 
GGCCCGCCCC AACCTGGAGA CTCTGAGCTG CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAAGC CCGGGGCCGG CGAAGGCGCC 
GGGGAGAATT GGGAGCCGCG CGTCTTGCCC TACCACCCTG CACAGCCCGG CCAGGCCGCC 
AAAAAGGCCG TCAGGACCCG CTACATCAGC ACGGAGCTGG GCATCAGGCA GAGGCTGCTG 
GTGGCGGTGC TGACCTCTCA GACCACGCTG CCCACGCTGG GCGTGGCCGT GAACCGCACG 
CTGGGGCACC GGCTGGAGCG TGTGGTGTTC CTGACGGGCG CACGGGGCCG CCGGGCCCCA 
CCTGGCATGG CAGTGGTGAC GCTAGGCGAG GAGCGACCCA TTGGACACCT GCACCTGGCG 
CTGCGCCACC TGCTGGAGCA GCACGGCGAC GACTTTGACT GGTTCTTCCT GGTGCCTGAC 
ACCACCTACA CCGAGGCGCA CGGCCTGGCA CGCCTAACTG GCCACCTCAG CCTGGCCTCC 
GCCGCCCACC TGTACCTGGG CCGGCCCCAG GACTTCATCG GCGGAGAGCC CACCCCCGGC 
CGCTACTGCC ACGGAGGCTT TGGGGTGCTG CTGTCGCGCA TGCTGCTGCA ACAACTGCGC 
CCCCACCTGG AAGGCTGCCG CAACGACATC GTCAGTGCGC GCCCTGACGA GTGGCTGGGT 
CGCTGCATTC TCGATGCCAC CGGGGTGGGC TGCACTGGTG ACCACGAGGG GGTGCACTAT 
AGCCATCTGG AGCTGAGCCC TGGGGAGCCA GTGCAGGAGG GGGACCCTCA TTTCCGAAGT 
GCCCTGACAG CCCACCCTGT GCGTGACCCT GTGCACATGT ACCAGCTGCA CAAAGCTTTC 
GCCCGAGCTG AACTGGAACG CACGTACCAG GAGATCCAGG AGTTACAGTG GGAGATCCAG 
AATACCAGCC ATCTGGCCGT TGATGGGGAC CGGGCAGCTG CTTGGCCCGT GGGTATTCCA 
GCACCATCCC GCCCGGCCTC CCGCTTTGAG GTGCTGCGCT GGGACTACTT CACGGAGCAG 
CACGCTTTCT CCTGCGCCGA TGGCTCACCC CGCTGCCCAC TGCGTGGGGC TGACCGGGCT 
GATGTGGCCG ATGTTCTGGG GACAGCTCTA GAGGAGCTGA ACCGCCGCTA CCACCCGGCC 
TTGCGGCTCC AGAAGCAGCA GCTGGTGAAT GGCTACCGAC GCTTTGATCC GGCCCGGGGT 
ATGGAATACA CGCTGGACTT GCAGCTGGAG GCACTGACCC CCCAGGGAGG CCGCCGGCCC 
CTCACTCGCC GAGTGCAGCT GCTCCGGCCG CTGAGCCGCG TGGAGATCTT GCCTGTGCCC 
TATGTCACTG AGGCCTCACG TCTCACTGTG CTGCTGCCTC TAGCTGCGGC TGAGCGTGAC 
CTGGCCCCTG GCTTCTTGGA GGCCTTTGCC ACTGCAGCAC TGGAGCCTGG TGATGCTGCG 
GCAGCCCTGA CCCTGCTGCT ACTGTATGAG CCGCGCCAGG CCCAGCGCGT GGCCCATGCA 
GATGTCTTCG CACCTGTCAA GGCCCACGTG GCAGAGCTGG AGCGGCGTTT CCCCGGTGCC 
CGGGTGCCAT GGCTCAGTGT GCAGACAGCC GCACCCTCAC CACTGCGCCT CATGGATCTA 
CTCTCCAAGA AGCACCCGCT GGACACACTG TTCCTGCTGG CCGGGCCAGA CACGGTGCTC 
ACGCCTGACT TCCTGAACCG CTGCCGCATG CATGCCATCT CCGGCTGGCA GGCCTTCTTT 
CCCATGCATT TCCAAGCCTT CCACCCAGCT GTGGCCCCAC CACAAGGGCC TGGGCCCCCA 
GAGCTGGGCC GTGACACTGG CCGCTTTGAT CGCCAGGCAG CCAGCGAGGC CTGCTTCTAC 
AACTCCGACT ACGTGGCAGC CCGTGGGCGC CTGGCGGCAG CCTCAGAACA AGAAGAGGAG 
CTGCTGGAGA GCCTGGATGT GTACGAGCTG TTCCTCCACT TCTCCAGTCT GCATGTGCTG 
CGGGCGGTGG AGCCGGCGCT GCTGCAGCGC TACCGGGCCC AGACGTGCAG CGCGAGGCTC 
AGTGAGGACC TGTACCACCG CTGCCTCCAG AGCGTGCTTG AGGGCCTCGG CTCCCGAACC 
CAGCTGGCCA TGCTACTCTT TGAACAGGAG CAGGGCAACA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHPF ( NM_024536.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_078812.3 Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107333
Clone ID Related Accession (Same CDS sequence)	NM_024536.6
Accession Version	NM_024536.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009955.4. On Nov 23, 2018 this sequence version replaced NM_024536.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.369180.1, SRR3476690.908193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000243776.11/ ENSP00000243776.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGCAT CGCTGCTGCT GTCGGTGCTG CGGCCCGCAG GGCCCGTGGC CGTGGGCATC 
TCCCTGGGCT TCACCCTGAG CCTGCTCAGC GTCACCTGGG TGGAGGAGCC GTGCGGCCCA 
GGCCCGCCCC AACCTGGAGA CTCTGAGCTG CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAAGC CCGGGGCCGG CGAAGGCGCC 
GGGGAGAATT GGGAGCCGCG CGTCTTGCCC TACCACCCTG CACAGCCCGG CCAGGCCGCC 
AAAAAGGCCG TCAGGACCCG CTACATCAGC ACGGAGCTGG GCATCAGGCA GAGGCTGCTG 
GTGGCGGTGC TGACCTCTCA GACCACGCTG CCCACGCTGG GCGTGGCCGT GAACCGCACG 
CTGGGGCACC GGCTGGAGCG TGTGGTGTTC CTGACGGGCG CACGGGGCCG CCGGGCCCCA 
CCTGGCATGG CAGTGGTGAC GCTAGGCGAG GAGCGACCCA TTGGACACCT GCACCTGGCG 
CTGCGCCACC TGCTGGAGCA GCACGGCGAC GACTTTGACT GGTTCTTCCT GGTGCCTGAC 
ACCACCTACA CCGAGGCGCA CGGCCTGGCA CGCCTAACTG GCCACCTCAG CCTGGCCTCC 
GCCGCCCACC TGTACCTGGG CCGGCCCCAG GACTTCATCG GCGGAGAGCC CACCCCCGGC 
CGCTACTGCC ACGGAGGCTT TGGGGTGCTG CTGTCGCGCA TGCTGCTGCA ACAACTGCGC 
CCCCACCTGG AAGGCTGCCG CAACGACATC GTCAGTGCGC GCCCTGACGA GTGGCTGGGT 
CGCTGCATTC TCGATGCCAC CGGGGTGGGC TGCACTGGTG ACCACGAGGG GGTGCACTAT 
AGCCATCTGG AGCTGAGCCC TGGGGAGCCA GTGCAGGAGG GGGACCCTCA TTTCCGAAGT 
GCCCTGACAG CCCACCCTGT GCGTGACCCT GTGCACATGT ACCAGCTGCA CAAAGCTTTC 
GCCCGAGCTG AACTGGAACG CACGTACCAG GAGATCCAGG AGTTACAGTG GGAGATCCAG 
AATACCAGCC ATCTGGCCGT TGATGGGGAC CAGGCAGCTG CTTGGCCCGT GGGTATTCCA 
GCACCATCCC GCCCGGCCTC CCGCTTTGAG GTGCTGCGCT GGGACTACTT CACGGAGCAG 
CACGCTTTCT CCTGCGCCGA TGGCTCACCC CGCTGCCCAC TGCGTGGGGC TGACCGGGCT 
GATGTGGCCG ATGTTCTGGG GACAGCTCTA GAGGAGCTGA ACCGCCGCTA CCACCCGGCC 
TTGCGGCTCC AGAAGCAGCA GCTGGTGAAT GGCTACCGAC GCTTTGATCC GGCCCGGGGT 
ATGGAATACA CGCTGGACTT GCAGCTGGAG GCACTGACCC CCCAGGGAGG CCGCCGGCCC 
CTCACTCGCC GAGTGCAGCT GCTCCGGCCG CTGAGCCGCG TGGAGATCTT GCCTGTGCCC 
TATGTCACTG AGGCCTCACG TCTCACTGTG CTGCTGCCTC TAGCTGCGGC TGAGCGTGAC 
CTGGCCCCTG GCTTCTTGGA GGCCTTTGCC ACTGCAGCAC TGGAGCCTGG TGATGCTGCG 
GCAGCCCTGA CCCTGCTGCT ACTGTATGAG CCGCGCCAGG CCCAGCGCGT GGCCCATGCA 
GATGTCTTCG CACCTGTCAA GGCCCACGTG GCAGAGCTGG AGCGGCGTTT CCCCGGTGCC 
CGGGTGCCAT GGCTCAGTGT GCAGACAGCC GCACCCTCAC CACTGCGCCT CATGGATCTA 
CTCTCCAAGA AGCACCCGCT GGACACACTG TTCCTGCTGG CCGGGCCAGA CACGGTGCTC 
ACGCCTGACT TCCTGAACCG CTGCCGCATG CATGCCATCT CCGGCTGGCA GGCCTTCTTT 
CCCATGCATT TCCAAGCCTT CCACCCAGCT GTGGCCCCAC CACAAGGGCC TGGGCCCCCA 
GAGCTGGGCC GTGACACTGG CCGCTTTGAT CGCCAGGCAG CCAGCGAGGC CTGCTTCTAC 
AACTCCGACT ATGTGGCAGC CCGTGGGCGC CTGGCGGCAG CCTCAGAACA AGAAGAGGAG 
CTGCTGGAGA GCCTGGATGT GTACGAGCTG TTCCTCCACT TCTCCAGTCT GCATGTGCTG 
CGGGCGGTGG AGCCGGCGCT GCTGCAGCGC TACCGGGCCC AGACGTGCAG CGCGAGGCTC 
AGTGAGGACC TGTACCACCG CTGCCTCCAG AGCGTGCTTG AGGGCCTCGG CTCCCGAACC 
CAGCTGGCCA TGCTACTCTT TGAACAGGAG CAGGGCAACA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078812.3
CDS	272..2599
Translation	MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGAEREKPGAGEGAGENWEPRVLPYHPAQPGQAAKKAVRTRYISTELGIRQRLLVAVLTSQTTLPTLGVAVNRTLGHRLERVVFLTGARGRRAPPGMAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSLASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	NM_024536.6
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024536.6

ORF Insert Sequence:

ATGCGGGCAT CGCTGCTGCT GTCGGTGCTG CGGCCCGCAG GGCCCGTGGC CGTGGGCATC 
TCCCTGGGCT TCACCCTGAG CCTGCTCAGC GTCACCTGGG TGGAGGAGCC GTGCGGCCCA 
GGCCCGCCCC AACCTGGAGA CTCTGAGCTG CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAAGC CCGGGGCCGG CGAAGGCGCC 
GGGGAGAATT GGGAGCCGCG CGTCTTGCCC TACCACCCTG CACAGCCCGG CCAGGCCGCC 
AAAAAGGCCG TCAGGACCCG CTACATCAGC ACGGAGCTGG GCATCAGGCA GAGGCTGCTG 
GTGGCGGTGC TGACCTCTCA GACCACGCTG CCCACGCTGG GCGTGGCCGT GAACCGCACG 
CTGGGGCACC GGCTGGAGCG TGTGGTGTTC CTGACGGGCG CACGGGGCCG CCGGGCCCCA 
CCTGGCATGG CAGTGGTGAC GCTAGGCGAG GAGCGACCCA TTGGACACCT GCACCTGGCG 
CTGCGCCACC TGCTGGAGCA GCACGGCGAC GACTTTGACT GGTTCTTCCT GGTGCCTGAC 
ACCACCTACA CCGAGGCGCA CGGCCTGGCA CGCCTAACTG GCCACCTCAG CCTGGCCTCC 
GCCGCCCACC TGTACCTGGG CCGGCCCCAG GACTTCATCG GCGGAGAGCC CACCCCCGGC 
CGCTACTGCC ACGGAGGCTT TGGGGTGCTG CTGTCGCGCA TGCTGCTGCA ACAACTGCGC 
CCCCACCTGG AAGGCTGCCG CAACGACATC GTCAGTGCGC GCCCTGACGA GTGGCTGGGT 
CGCTGCATTC TCGATGCCAC CGGGGTGGGC TGCACTGGTG ACCACGAGGG GGTGCACTAT 
AGCCATCTGG AGCTGAGCCC TGGGGAGCCA GTGCAGGAGG GGGACCCTCA TTTCCGAAGT 
GCCCTGACAG CCCACCCTGT GCGTGACCCT GTGCACATGT ACCAGCTGCA CAAAGCTTTC 
GCCCGAGCTG AACTGGAACG CACGTACCAG GAGATCCAGG AGTTACAGTG GGAGATCCAG 
AATACCAGCC ATCTGGCCGT TGATGGGGAC CAGGCAGCTG CTTGGCCCGT GGGTATTCCA 
GCACCATCCC GCCCGGCCTC CCGCTTTGAG GTGCTGCGCT GGGACTACTT CACGGAGCAG 
CACGCTTTCT CCTGCGCCGA TGGCTCACCC CGCTGCCCAC TGCGTGGGGC TGACCGGGCT 
GATGTGGCCG ATGTTCTGGG GACAGCTCTA GAGGAGCTGA ACCGCCGCTA CCACCCGGCC 
TTGCGGCTCC AGAAGCAGCA GCTGGTGAAT GGCTACCGAC GCTTTGATCC GGCCCGGGGT 
ATGGAATACA CGCTGGACTT GCAGCTGGAG GCACTGACCC CCCAGGGAGG CCGCCGGCCC 
CTCACTCGCC GAGTGCAGCT GCTCCGGCCG CTGAGCCGCG TGGAGATCTT GCCTGTGCCC 
TATGTCACTG AGGCCTCACG TCTCACTGTG CTGCTGCCTC TAGCTGCGGC TGAGCGTGAC 
CTGGCCCCTG GCTTCTTGGA GGCCTTTGCC ACTGCAGCAC TGGAGCCTGG TGATGCTGCG 
GCAGCCCTGA CCCTGCTGCT ACTGTATGAG CCGCGCCAGG CCCAGCGCGT GGCCCATGCA 
GATGTCTTCG CACCTGTCAA GGCCCACGTG GCAGAGCTGG AGCGGCGTTT CCCCGGTGCC 
CGGGTGCCAT GGCTCAGTGT GCAGACAGCC GCACCCTCAC CACTGCGCCT CATGGATCTA 
CTCTCCAAGA AGCACCCGCT GGACACACTG TTCCTGCTGG CCGGGCCAGA CACGGTGCTC 
ACGCCTGACT TCCTGAACCG CTGCCGCATG CATGCCATCT CCGGCTGGCA GGCCTTCTTT 
CCCATGCATT TCCAAGCCTT CCACCCAGCT GTGGCCCCAC CACAAGGGCC TGGGCCCCCA 
GAGCTGGGCC GTGACACTGG CCGCTTTGAT CGCCAGGCAG CCAGCGAGGC CTGCTTCTAC 
AACTCCGACT ATGTGGCAGC CCGTGGGCGC CTGGCGGCAG CCTCAGAACA AGAAGAGGAG 
CTGCTGGAGA GCCTGGATGT GTACGAGCTG TTCCTCCACT TCTCCAGTCT GCATGTGCTG 
CGGGCGGTGG AGCCGGCGCT GCTGCAGCGC TACCGGGCCC AGACGTGCAG CGCGAGGCTC 
AGTGAGGACC TGTACCACCG CTGCCTCCAG AGCGTGCTTG AGGGCCTCGG CTCCCGAACC 
CAGCTGGCCA TGCTACTCTT TGAACAGGAG CAGGGCAACA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TGF-? Stimulates Expression of Chondroitin Polymerizing Factor in Nucleus Pulposus Cells Through the Smad3, RhoA/ROCK1, and MAPK Signaling Pathways.
Journal of cellular biochemistry119(1)566-579(2018 Jan)
Hu B,Xu C,Cao P,Tian Y,Zhang Y,Shi C,Xu J,Yuan W,Chen H

Lentivirus?mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in?vitro.
Oncology reports38(2)1149-1155(2017 Aug)
Fan YH,Xiao B,Lv SG,Ye MH,Zhu XG,Wu MJ

Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential.
Human molecular genetics21(5)991-1003(2012 Mar)
Kuroda Y,Sako W,Goto S,Sawada T,Uchida D,Izumi Y,Takahashi T,Kagawa N,Matsumoto M,Matsumoto M,Takahashi R,Kaji R,Mitsui T

FOXM1 induces a global methylation signature that mimics the cancer epigenome in head and neck squamous cell carcinoma.
PloS one7(3)e34329(2012)
Teh MT,Gemenetzidis E,Patel D,Tariq R,Nadir A,Bahta AW,Waseem A,Hutchison IL

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CHPF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報