NELFE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFE
Entrez Gene ID	7936
Full Name	negative elongation factor complex member E
Synonyms	D6S45,NELF-E,RD,RDBP,RDP
General protein information	Preferred Names negative elongation factor complex member E Names negative elongation factor E RD RNA-binding protein RNA-binding protein RD major histocompatibility complex gene RD negative elongation factor polypeptide E nuclear protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008].
Disorder MIM:	154040

mRNA and Protein(s)

mRNA	Protein	Name
XM_017011299.2	XP_016866788.1	negative elongation factor E isoform X3
XM_011514913.2	XP_011513215.1	negative elongation factor E isoform X2
XM_006715205.3	XP_006715268.1	negative elongation factor E isoform X1
XM_011514913.3	XP_011513215.1	negative elongation factor E isoform X2
XM_017011299.1	XP_016866788.1	negative elongation factor E isoform X3
NM_002904.5	NP_002895.3	negative elongation factor E
NM_002904.6	NP_002895.3	negative elongation factor E

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	nelfe	NP_001122129.1
Homo sapiens (human)	NELFE	NP_002895.3
	RDBP	XP_002803722.1
Canis lupus familiaris (dog)	NELFE	XP_532087.1
Bos taurus (cattle)	NELFE	NP_001069672.1
Mus musculus (house mouse)	Nelfe	NP_001039328.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for mutually exclusive co-transcriptional nuclear cap-binding complexes with either NELF-E or ARS2.
Schulze WM, Cusack S
Nature communications8(1)1302(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Architecture and RNA binding of the human negative elongation factor.
Vos SM, P?llmann D, Caizzi L, Hofmann KB, Rombaut P, Zimniak T, Herzog F, Cramer P
eLife5(2016 06)

Kinetically Defined Mechanisms and Positions of Action of Two New Modulators of Glucocorticoid Receptor-regulated Gene Induction.
Pradhan MA, Blackford JA Jr, Devaiah BN, Thompson PS, Chow CC, Singer DS, Simons SS Jr
The Journal of biological chemistry291(1)342-54(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.
Title: Architecture and RNA binding of the human negative elongation factor.

novel actions of BRD4 and of NELF-E in GR-controlled gene induction have been uncovered.
Title: Kinetically Defined Mechanisms and Positions of Action of Two New Modulators of Glucocorticoid Receptor-regulated Gene Induction.

Following NELF-E knockdown or tumor necrosis factor alpha stimulation, promoter-proximal RNAP II levels increase up to 3-fold, and there is a dramatic increase in RNAP II levels within the HIV genome.
Title: Negative elongation factor is required for the maintenance of proviral latency but does not induce promoter-proximal pausing of RNA polymerase II on the HIV long terminal repeat.

these results describe the RNA binding behavior of NELF-E and supports a biological role for NELF-E in promoter-proximal pausing of both HIV-1 and cellular genes.
Title: Defining NELF-E RNA binding in HIV-1 and promoter-proximal pause regions.

the transcription elongation of KSHV OriLytL-K7 lytic genes is inhibited by NELF during latency, but can also be promptly reactivated in an RTA-independent manner upon external stimuli
Title: Negative elongation factor-mediated suppression of RNA polymerase II elongation of Kaposi's sarcoma-associated herpesvirus lytic gene expression.

The following NELFE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25470	NM_002904.6 Latest version!	Homo sapiens negative elongation factor complex member E (NELFE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu40473	XM_006715205.3 Latest version!	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu61561	XM_011514913.3 Latest version!	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu96803	XM_017011299.2 Latest version!	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu61561	XM_011514913.2	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu96803	XM_017011299.1	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu25470	NM_002904.5	Homo sapiens negative elongation factor complex member E (NELFE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFE ( NM_002904.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002895.3 Homo sapiens negative elongation factor complex member E (NELFE), mRNA.

Price & Availability↑

CloneID	OHu25470
Clone ID Related Accession (Same CDS sequence)	NM_002904.5 , NM_002904.6
Accession Version	NM_002904.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	negative elongation factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD579634.2, BC050617.2 and BU935034.1. On Jun 17, 2005 this sequence version replaced NM_002904.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.278559.1, SRR1163657.193053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGCTTTGT GTCTTCTAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002895.3
CDS	205..1347
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	NM_002904.5
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002904.5

ORF Insert Sequence:

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGCTTTGT GTCTTCTAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( NM_002904.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002895.3 Homo sapiens negative elongation factor complex member E (NELFE), mRNA.

Price & Availability↑

CloneID	OHu25470
Clone ID Related Accession (Same CDS sequence)	NM_002904.5 , NM_002904.6
Accession Version	NM_002904.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	negative elongation factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD579634.2, BC050617.2 and BU935034.1. On Nov 22, 2018 this sequence version replaced NM_002904.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.67520.1, SRR1163658.484386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375429.8/ ENSP00000364578.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGCTTTGT GTCTTCTAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002895.3
CDS	89..1231
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	NM_002904.6
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002904.6

ORF Insert Sequence:

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGCTTTGT GTCTTCTAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_006715205.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715268.1 Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu40473
Clone ID Related Accession (Same CDS sequence)	XM_006715205.3
Accession Version	XM_006715205.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	negative elongation factor E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGTGATCG ACTTCGAGAA 
CTAGGACCAG ATGGAGAAGA GGCAGAGGGC CCAGGGGCTG GTGATGGTCC CCCTCGAAGC 
TTTGACTGGG GCTATGAAGA ACGCAGTGGT GCCCACTCCT CAGCCTCCCC TCCCCGAAGC 
CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC CGGGACAGAG ACCGAGATCG GGAGCGGGAT 
CGAGACCGGG ATCGAGACAG AGACAGAGAG CGGGACAGGG ATCGGGATCG GGATCGAGAT 
CGAGACCGGG AACGGGACAG GGATCGGGAG CGGGATCGAG ACCGAGACCG AGAGGGTCCT 
TTCCGCAGGT CGGATTCATT CCCTGAACGG CGAGCCCCTA GGAAAGGGAA TACTCTCTAT 
GTATATGGAG AAGACATGAC ACCCACCCTT CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC 
ATCATTGACC TCTCCATGGA CCCACCCAGA AACTGTGCCT TCGTCACCTA TGAAAAGATG 
GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC AACGGGACCC AGGTGGAGTC TGTACAGCTC 
AAAGTCAACA TAGCCCGAAA ACAGCCCATG CTGGATGCCG CTACTGGCAA GTCTGTCTGG 
GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG GGTTGCCACC GGGACAAGAG GACCCAGATT 
GTCTACAGTG ATGACGTCTA CAAGGAAAAC CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715268.1
CDS	89..1216
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_006715205.3
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715205.3

ORF Insert Sequence:

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAAGAGGAGG CTCTGCAGAA GAAATTCAAC 
AAGCTCAAGA AAAAGAAAAA GGCATTGCTG GCTCTGAAGA AGCAAAGTAG CAGCAGCACA 
ACCAGCCAAG GTGGTGTCAA ACGCTCACTA TCAGAGCAGC CTGTCATGGA CACAGCCACA 
GCAACAGAGC AGGCAAAGCA GCTGGTGAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA GCGTTCTCGA ACCCTTGAGG GGAAGTTAAA GGACCCCGAG 
AAGGGACCAG TCCCCACTTT CCAGCCGTTC CAGAGGAGCA TATCTGCTGA TGATGACCTG 
CAAGAGTCAT CCAGACGTCC CCAGAGGAAA TCTCTGTATG AGAGTGATCG ACTTCGAGAA 
CTAGGACCAG ATGGAGAAGA GGCAGAGGGC CCAGGGGCTG GTGATGGTCC CCCTCGAAGC 
TTTGACTGGG GCTATGAAGA ACGCAGTGGT GCCCACTCCT CAGCCTCCCC TCCCCGAAGC 
CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC CGGGACAGAG ACCGAGATCG GGAGCGGGAT 
CGAGACCGGG ATCGAGACAG AGACAGAGAG CGGGACAGGG ATCGGGATCG GGATCGAGAT 
CGAGACCGGG AACGGGACAG GGATCGGGAG CGGGATCGAG ACCGAGACCG AGAGGGTCCT 
TTCCGCAGGT CGGATTCATT CCCTGAACGG CGAGCCCCTA GGAAAGGGAA TACTCTCTAT 
GTATATGGAG AAGACATGAC ACCCACCCTT CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC 
ATCATTGACC TCTCCATGGA CCCACCCAGA AACTGTGCCT TCGTCACCTA TGAAAAGATG 
GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC AACGGGACCC AGGTGGAGTC TGTACAGCTC 
AAAGTCAACA TAGCCCGAAA ACAGCCCATG CTGGATGCCG CTACTGGCAA GTCTGTCTGG 
GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG GGTTGCCACC GGGACAAGAG GACCCAGATT 
GTCTACAGTG ATGACGTCTA CAAGGAAAAC CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_011514913.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513215.1 Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61561
Clone ID Related Accession (Same CDS sequence)	XM_011514913.2 , XM_011514913.3
Accession Version	XM_011514913.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	negative elongation factor E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGC 
TTTGTGTCTT CTAGTGATCG ACTTCGAGAA CTAGGACCAG ATGGAGAAGA GGCAGAGGGC 
CCAGGGGCTG GTGATGGTCC CCCTCGAAGC TTTGACTGGG GCTATGAAGA ACGCAGTGGT 
GCCCACTCCT CAGCCTCCCC TCCCCGAAGC CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC 
CGGGACAGAG ACCGAGATCG GGAGCGGGAT CGAGACCGGG ATCGAGACAG AGACAGAGAG 
CGGGACAGGG ATCGGGATCG GGATCGAGAT CGAGACCGGG AACGGGACAG GGATCGGGAG 
CGGGATCGAG ACCGAGACCG AGAGGGTCCT TTCCGCAGGT CGGATTCATT CCCTGAACGG 
CGAGCCCCTA GGAAAGGGAA TACTCTCTAT GTATATGGAG AAGACATGAC ACCCACCCTT 
CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC ATCATTGACC TCTCCATGGA CCCACCCAGA 
AACTGTGCCT TCGTCACCTA TGAAAAGATG GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC 
AACGGGACCC AGGTGGAGTC TGTACAGCTC AAAGTCAACA TAGCCCGAAA ACAGCCCATG 
CTGGATGCCG CTACTGGCAA GTCTGTCTGG GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG 
GGTTGCCACC GGGACAAGAG GACCCAGATT GTCTACAGTG ATGACGTCTA CAAGGAAAAC 
CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513215.1
CDS	310..1287
Translation	MDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_011514913.2
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514913.2

ORF Insert Sequence:

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGC 
TTTGTGTCTT CTAGTGATCG ACTTCGAGAA CTAGGACCAG ATGGAGAAGA GGCAGAGGGC 
CCAGGGGCTG GTGATGGTCC CCCTCGAAGC TTTGACTGGG GCTATGAAGA ACGCAGTGGT 
GCCCACTCCT CAGCCTCCCC TCCCCGAAGC CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC 
CGGGACAGAG ACCGAGATCG GGAGCGGGAT CGAGACCGGG ATCGAGACAG AGACAGAGAG 
CGGGACAGGG ATCGGGATCG GGATCGAGAT CGAGACCGGG AACGGGACAG GGATCGGGAG 
CGGGATCGAG ACCGAGACCG AGAGGGTCCT TTCCGCAGGT CGGATTCATT CCCTGAACGG 
CGAGCCCCTA GGAAAGGGAA TACTCTCTAT GTATATGGAG AAGACATGAC ACCCACCCTT 
CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC ATCATTGACC TCTCCATGGA CCCACCCAGA 
AACTGTGCCT TCGTCACCTA TGAAAAGATG GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC 
AACGGGACCC AGGTGGAGTC TGTACAGCTC AAAGTCAACA TAGCCCGAAA ACAGCCCATG 
CTGGATGCCG CTACTGGCAA GTCTGTCTGG GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG 
GGTTGCCACC GGGACAAGAG GACCCAGATT GTCTACAGTG ATGACGTCTA CAAGGAAAAC 
CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_011514913.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513215.1 Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61561
Clone ID Related Accession (Same CDS sequence)	XM_011514913.2 , XM_011514913.3
Accession Version	XM_011514913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	negative elongation factor E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGC 
TTTGTGTCTT CTAGTGATCG ACTTCGAGAA CTAGGACCAG ATGGAGAAGA GGCAGAGGGC 
CCAGGGGCTG GTGATGGTCC CCCTCGAAGC TTTGACTGGG GCTATGAAGA ACGCAGTGGT 
GCCCACTCCT CAGCCTCCCC TCCCCGAAGC CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC 
CGGGACAGAG ACCGAGATCG GGAGCGGGAT CGAGACCGGG ATCGAGACAG AGACAGAGAG 
CGGGACAGGG ATCGGGATCG GGATCGAGAT CGAGACCGGG AACGGGACAG GGATCGGGAG 
CGGGATCGAG ACCGAGACCG AGAGGGTCCT TTCCGCAGGT CGGATTCATT CCCTGAACGG 
CGAGCCCCTA GGAAAGGGAA TACTCTCTAT GTATATGGAG AAGACATGAC ACCCACCCTT 
CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC ATCATTGACC TCTCCATGGA CCCACCCAGA 
AACTGTGCCT TCGTCACCTA TGAAAAGATG GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC 
AACGGGACCC AGGTGGAGTC TGTACAGCTC AAAGTCAACA TAGCCCGAAA ACAGCCCATG 
CTGGATGCCG CTACTGGCAA GTCTGTCTGG GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG 
GGTTGCCACC GGGACAAGAG GACCCAGATT GTCTACAGTG ATGACGTCTA CAAGGAAAAC 
CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513215.1
CDS	387..1364
Translation	MDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_011514913.3
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514913.3

ORF Insert Sequence:

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGC 
TTTGTGTCTT CTAGTGATCG ACTTCGAGAA CTAGGACCAG ATGGAGAAGA GGCAGAGGGC 
CCAGGGGCTG GTGATGGTCC CCCTCGAAGC TTTGACTGGG GCTATGAAGA ACGCAGTGGT 
GCCCACTCCT CAGCCTCCCC TCCCCGAAGC CGCAGCCGGG ACCGCAGCCA TGAGAGGAAC 
CGGGACAGAG ACCGAGATCG GGAGCGGGAT CGAGACCGGG ATCGAGACAG AGACAGAGAG 
CGGGACAGGG ATCGGGATCG GGATCGAGAT CGAGACCGGG AACGGGACAG GGATCGGGAG 
CGGGATCGAG ACCGAGACCG AGAGGGTCCT TTCCGCAGGT CGGATTCATT CCCTGAACGG 
CGAGCCCCTA GGAAAGGGAA TACTCTCTAT GTATATGGAG AAGACATGAC ACCCACCCTT 
CTCCGTGGGG CCTTCTCTCC TTTTGGAAAC ATCATTGACC TCTCCATGGA CCCACCCAGA 
AACTGTGCCT TCGTCACCTA TGAAAAGATG GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC 
AACGGGACCC AGGTGGAGTC TGTACAGCTC AAAGTCAACA TAGCCCGAAA ACAGCCCATG 
CTGGATGCCG CTACTGGCAA GTCTGTCTGG GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG 
GGTTGCCACC GGGACAAGAG GACCCAGATT GTCTACAGTG ATGACGTCTA CAAGGAAAAC 
CTTGTGGATG GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_017011299.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866788.1 Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu96803
Clone ID Related Accession (Same CDS sequence)	XM_017011299.2 , XM_017011299.1
Accession Version	XM_017011299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	negative elongation factor E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866788.1
CDS	390..1352
Translation	MDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_017011299.2
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011299.2

ORF Insert Sequence:

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_017011299.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866788.1 Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu96803
Clone ID Related Accession (Same CDS sequence)	XM_017011299.2 , XM_017011299.1
Accession Version	XM_017011299.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	negative elongation factor E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866788.1
CDS	310..1272
Translation	MDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_017011299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member E (NELFE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011299.1

ORF Insert Sequence:

ATGGACACAG CCACAGCAAC AGAGCAGGCA AAGCAGCTGG TGAAGTCAGG AGCCATCAGT 
GCCATCAAGG CTGAGACCAA GAACTCAGGC TTCAAGCGTT CTCGAACCCT TGAGGGGAAG 
TTAAAGGACC CCGAGAAGGG ACCAGTCCCC ACTTTCCAGC CGTTCCAGAG GAGCATATCT 
GCTGATGATG ACCTGCAAGA GTCATCCAGA CGTCCCCAGA GGAAATCTCT GTATGAGAGT 
GATCGACTTC GAGAACTAGG ACCAGATGGA GAAGAGGCAG AGGGCCCAGG GGCTGGTGAT 
GGTCCCCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGCA GTGGTGCCCA CTCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACCGC AGCCATGAGA GGAACCGGGA CAGAGACCGA 
GATCGGGAGC GGGATCGAGA CCGGGATCGA GACAGAGACA GAGAGCGGGA CAGGGATCGG 
GATCGGGATC GAGATCGAGA CCGGGAACGG GACAGGGATC GGGAGCGGGA TCGAGACCGA 
GACCGAGAGG GTCCTTTCCG CAGGTCGGAT TCATTCCCTG AACGGCGAGC CCCTAGGAAA 
GGGAATACTC TCTATGTATA TGGAGAAGAC ATGACACCCA CCCTTCTCCG TGGGGCCTTC 
TCTCCTTTTG GAAACATCAT TGACCTCTCC ATGGACCCAC CCAGAAACTG TGCCTTCGTC 
ACCTATGAAA AGATGGAGTC AGCAGATCAG GCCGTTGCTG AGCTCAACGG GACCCAGGTG 
GAGTCTGTAC AGCTCAAAGT CAACATAGCC CGAAAACAGC CCATGCTGGA TGCCGCTACT 
GGCAAGTCTG TCTGGGGCTC CCTCGCTGTC CAGAACAGCC CTAAGGGTTG CCACCGGGAC 
AAGAGGACCC AGATTGTCTA CAGTGATGAC GTCTACAAGG AAAACCTTGT GGATGGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for mutually exclusive co-transcriptional nuclear cap-binding complexes with either NELF-E or ARS2.
Nature communications8(1)1302(2017 Nov)
Schulze WM,Cusack S

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Architecture and RNA binding of the human negative elongation factor.
eLife5(2016 06)
Vos SM,P?llmann D,Caizzi L,Hofmann KB,Rombaut P,Zimniak T,Herzog F,Cramer P

Kinetically Defined Mechanisms and Positions of Action of Two New Modulators of Glucocorticoid Receptor-regulated Gene Induction.
The Journal of biological chemistry291(1)342-54(2016 Jan)
Pradhan MA,Blackford JA Jr,Devaiah BN,Thompson PS,Chow CC,Singer DS,Simons SS Jr

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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NELFE cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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