HSD17B8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSD17B8
Entrez Gene ID	7923
Full Name	hydroxysteroid 17-beta dehydrogenase 8
Synonyms	D6S2245E,FABG,FABGL,H2-KE6,HKE6,KE6,RING2,SDR30C1,dJ1033B10.9
General protein information	Preferred Names hydroxysteroid 17-beta dehydrogenase 8 Names estradiol 17-beta-dehydrogenase 8 17-beta-HSD 8 17-beta-hydroxysteroid dehydrogenase 8 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit 3-oxoacyl-[acyl-carrier-protein] reductase KAR alpha subunit estrogen 17-oxidoreductase ke-6 protein Ke6 really interesting new gene 2 protein short chain dehydrogenase/reductase family 30C member 1 testosterone 17-beta-dehydrogenase 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	601417

mRNA and Protein(s)

mRNA	Protein	Name
NM_014234.4	NP_055049.1	estradiol 17-beta-dehydrogenase 8
NM_014234.5	NP_055049.1	estradiol 17-beta-dehydrogenase 8

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	H2-Ke6	NP_038571.2
Rattus norvegicus (Norway rat)	Hsd17b8	NP_997694.1
Danio rerio (zebrafish)	hsd17b8	NP_001005292.2
Caenorhabditis elegans (roundworm)	dhs-25	NP_508282.2
	HSD17B8	NP_001108430.1
Bos taurus (cattle)	HSD17B8	NP_001039789.1
Drosophila melanogaster (fruit fly)	CG3603	NP_001259199.1
Homo sapiens (human)	HSD17B8	NP_055049.1
Oryza sativa (rice)	Os04g0376300	NP_001052580.1
Pan troglodytes (chimpanzee)	HSD17B8	XP_001169236.1
Canis lupus familiaris (dog)	HSD17B8	XP_851004.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009633	XP_318664.3
Caenorhabditis elegans (roundworm)	dhs-11	NP_499346.1
Xenopus tropicalis (tropical clawed frog)	hsd17b8	NP_001016671.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase.
Venkatesan R, Sah-Teli SK, Awoniyi LO, Jiang G, Prus P, Kastaniotis AJ, Hiltunen JK, Wierenga RK, Chen Z
Nature communications54805(2014 Sep)

Association of granulomatosis with polyangiitis (Wegener's) with HLA-DPB1*04 and SEMA6A gene variants: evidence from genome-wide analysis.
Xie G, Roshandel D, Sherva R, Monach PA, Lu EY, Kung T, Carrington K, Zhang SS, Pulit SL, Ripke S, Carette S, Dellaripa PF, Edberg JC, Hoffman GS, Khalidi N, Langford CA, Mahr AD, St Clair EW, Seo P, Specks U, Spiera RF, Stone JH, Ytterberg SR, Raychaudhuri S, de Bakker PI, Farrer LA, Amos CI, Merkel PA, Siminovitch KA
Arthritis and rheumatism65(9)2457-68(2013 Sep)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection.
Ucisik-Akkaya E, Davis CF, Do TN, Morrison BA, Stemmer SM, Amadio WJ, Dorak MT
Molecular human reproduction16(10)770-7(2010 Oct)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variation in the estrogen metabolic pathway and mammographic density as an intermediate phenotype of breast cancer.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following HSD17B8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSD17B8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu56064	NM_014234.5 Latest version!	Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu56064	NM_014234.4	Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSD17B8 ( NM_014234.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055049.1 Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.

Price & Availability↑

CloneID	OHu56064
Clone ID Related Accession (Same CDS sequence)	NM_014234.4 , NM_014234.5
Accession Version	NM_014234.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	estradiol 17-beta-dehydrogenase 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY167181.1, BC008185.1 and AW025057.1. On Jul 13, 2012 this sequence version replaced NM_014234.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.81525.1, BC008185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCTC AGCTCCAGAA CCGACTCCGC TCCGCACTGG CCTTGGTCAC AGGTGCGGGG 
AGCGGCATCG GCCGAGCGGT CAGTGTACGC CTGGCCGGAG AGGGGGCCAC CGTAGCTGCC 
TGCGACCTGG ACCGGGCAGC GGCACAGGAG ACGGTGCGGC TGCTGGGCGG GCCAGGGAGC 
AAGGAGGGGC CGCCCCGAGG GAACCATGCT GCCTTCCAGG CTGACGTGTC TGAGGCCAGG 
GCCGCCAGGT GCCTGCTGGA ACAAGTGCAG GCCTGCTTTT CTCGCCCACC ATCTGTCGTT 
GTGTCCTGTG CGGGCATCAC CCAGGATGAG TTTCTGCTGC ACATGTCTGA GGATGACTGG 
GACAAAGTCA TAGCTGTCAA CCTCAAGGGC ACCTTCCTAG TCACTCAGGC TGCAGCACAA 
GCCCTGGTGT CCAATGGTTG TCGTGGTTCC ATCATCAACA TCAGTAGCAT CGTAGGAAAG 
GTGGGGAACG TGGGGCAGAC AAACTATGCA GCATCCAAGG CTGGAGTGAT TGGGCTGACC 
CAGACCGCAG CCCGGGAGCT TGGACGACAT GGGATCCGCT GTAACTCTGT CCTCCCAGGG 
TTCATTGCAA CACCCATGAC ACAGAAAGTG CCACAGAAAG TGGTGGACAA GATTACTGAA 
ATGATCCCGA TGGGACACTT GGGGGACCCT GAGGATGTGG CAGATGTGGT CGCATTCTTG 
GCATCTGAAG ATAGTGGATA CATCACAGGG ACCTCAGTGG AAGTCACTGG AGGTCTTTTC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055049.1
CDS	33..818
Translation	MASQLQNRLRSALALVTGAGSGIGRAVSVRLAGEGATVAACDLDRAAAQETVRLLGGPGSKEGPPRGNHAAFQADVSEARAARCLLEQVQACFSRPPSVVVSCAGITQDEFLLHMSEDDWDKVIAVNLKGTFLVTQAAAQALVSNGCRGSIINISSIVGKVGNVGQTNYAASKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKVPQKVVDKITEMIPMGHLGDPEDVADVVAFLASEDSGYITGTSVEVTGGLFM

Target ORF information:

RefSeq Version	NM_014234.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014234.4

ORF Insert Sequence:

ATGGCGTCTC AGCTCCAGAA CCGACTCCGC TCCGCACTGG CCTTGGTCAC AGGTGCGGGG 
AGCGGCATCG GCCGAGCGGT CAGTGTACGC CTGGCCGGAG AGGGGGCCAC CGTAGCTGCC 
TGCGACCTGG ACCGGGCAGC GGCACAGGAG ACGGTGCGGC TGCTGGGCGG GCCAGGGAGC 
AAGGAGGGGC CGCCCCGAGG GAACCATGCT GCCTTCCAGG CTGACGTGTC TGAGGCCAGG 
GCCGCCAGGT GCCTGCTGGA ACAAGTGCAG GCCTGCTTTT CTCGCCCACC ATCTGTCGTT 
GTGTCCTGTG CGGGCATCAC CCAGGATGAG TTTCTGCTGC ACATGTCTGA GGATGACTGG 
GACAAAGTCA TAGCTGTCAA CCTCAAGGGC ACCTTCCTAG TCACTCAGGC TGCAGCACAA 
GCCCTGGTGT CCAATGGTTG TCGTGGTTCC ATCATCAACA TCAGTAGCAT CGTAGGAAAG 
GTGGGGAACG TGGGGCAGAC AAACTATGCA GCATCCAAGG CTGGAGTGAT TGGGCTGACC 
CAGACCGCAG CCCGGGAGCT TGGACGACAT GGGATCCGCT GTAACTCTGT CCTCCCAGGG 
TTCATTGCAA CACCCATGAC ACAGAAAGTG CCACAGAAAG TGGTGGACAA GATTACTGAA 
ATGATCCCGA TGGGACACTT GGGGGACCCT GAGGATGTGG CAGATGTGGT CGCATTCTTG 
GCATCTGAAG ATAGTGGATA CATCACAGGG ACCTCAGTGG AAGTCACTGG AGGTCTTTTC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSD17B8 ( NM_014234.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055049.1 Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.

Price & Availability↑

CloneID	OHu56064
Clone ID Related Accession (Same CDS sequence)	NM_014234.4 , NM_014234.5
Accession Version	NM_014234.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	estradiol 17-beta-dehydrogenase 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008185.1 and AW025057.1. On Nov 23, 2018 this sequence version replaced NM_014234.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.81525.1, BC008185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374662.4/ ENSP00000363794.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCTC AGCTCCAGAA CCGACTCCGC TCCGCACTGG CCTTGGTCAC AGGTGCGGGG 
AGCGGCATCG GCCGAGCGGT CAGTGTACGC CTGGCCGGAG AGGGGGCCAC CGTAGCTGCC 
TGCGACCTGG ACCGGGCAGC GGCACAGGAG ACGGTGCGGC TGCTGGGCGG GCCAGGGAGC 
AAGGAGGGGC CGCCCCGAGG GAACCATGCT GCCTTCCAGG CTGACGTGTC TGAGGCCAGG 
GCCGCCAGGT GCCTGCTGGA ACAAGTGCAG GCCTGCTTTT CTCGCCCACC ATCTGTCGTT 
GTGTCCTGTG CGGGCATCAC CCAGGATGAG TTTCTGCTGC ACATGTCTGA GGATGACTGG 
GACAAAGTCA TAGCTGTCAA CCTCAAGGGC ACCTTCCTAG TCACTCAGGC TGCAGCACAA 
GCCCTGGTGT CCAATGGTTG TCGTGGTTCC ATCATCAACA TCAGTAGCAT CGTAGGAAAG 
GTGGGGAACG TGGGGCAGAC AAACTATGCA GCATCCAAGG CTGGAGTGAT TGGGCTGACC 
CAGACCGCAG CCCGGGAGCT TGGACGACAT GGGATCCGCT GTAACTCTGT CCTCCCAGGG 
TTCATTGCAA CACCCATGAC ACAGAAAGTG CCACAGAAAG TGGTGGACAA GATTACTGAA 
ATGATCCCGA TGGGACACTT GGGGGACCCT GAGGATGTGG CAGATGTGGT CGCATTCTTG 
GCATCTGAAG ATAGTGGATA CATCACAGGG ACCTCAGTGG AAGTCACTGG AGGTCTTTTC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055049.1
CDS	15..800
Translation	MASQLQNRLRSALALVTGAGSGIGRAVSVRLAGEGATVAACDLDRAAAQETVRLLGGPGSKEGPPRGNHAAFQADVSEARAARCLLEQVQACFSRPPSVVVSCAGITQDEFLLHMSEDDWDKVIAVNLKGTFLVTQAAAQALVSNGCRGSIINISSIVGKVGNVGQTNYAASKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKVPQKVVDKITEMIPMGHLGDPEDVADVVAFLASEDSGYITGTSVEVTGGLFM

Target ORF information:

RefSeq Version	NM_014234.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxysteroid 17-beta dehydrogenase 8 (HSD17B8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014234.5

ORF Insert Sequence:

ATGGCGTCTC AGCTCCAGAA CCGACTCCGC TCCGCACTGG CCTTGGTCAC AGGTGCGGGG 
AGCGGCATCG GCCGAGCGGT CAGTGTACGC CTGGCCGGAG AGGGGGCCAC CGTAGCTGCC 
TGCGACCTGG ACCGGGCAGC GGCACAGGAG ACGGTGCGGC TGCTGGGCGG GCCAGGGAGC 
AAGGAGGGGC CGCCCCGAGG GAACCATGCT GCCTTCCAGG CTGACGTGTC TGAGGCCAGG 
GCCGCCAGGT GCCTGCTGGA ACAAGTGCAG GCCTGCTTTT CTCGCCCACC ATCTGTCGTT 
GTGTCCTGTG CGGGCATCAC CCAGGATGAG TTTCTGCTGC ACATGTCTGA GGATGACTGG 
GACAAAGTCA TAGCTGTCAA CCTCAAGGGC ACCTTCCTAG TCACTCAGGC TGCAGCACAA 
GCCCTGGTGT CCAATGGTTG TCGTGGTTCC ATCATCAACA TCAGTAGCAT CGTAGGAAAG 
GTGGGGAACG TGGGGCAGAC AAACTATGCA GCATCCAAGG CTGGAGTGAT TGGGCTGACC 
CAGACCGCAG CCCGGGAGCT TGGACGACAT GGGATCCGCT GTAACTCTGT CCTCCCAGGG 
TTCATTGCAA CACCCATGAC ACAGAAAGTG CCACAGAAAG TGGTGGACAA GATTACTGAA 
ATGATCCCGA TGGGACACTT GGGGGACCCT GAGGATGTGG CAGATGTGGT CGCATTCTTG 
GCATCTGAAG ATAGTGGATA CATCACAGGG ACCTCAGTGG AAGTCACTGG AGGTCTTTTC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase.
Nature communications54805(2014 Sep)
Venkatesan R,Sah-Teli SK,Awoniyi LO,Jiang G,Prus P,Kastaniotis AJ,Hiltunen JK,Wierenga RK,Chen Z

Association of granulomatosis with polyangiitis (Wegener's) with HLA-DPB1*04 and SEMA6A gene variants: evidence from genome-wide analysis.
Arthritis and rheumatism65(9)2457-68(2013 Sep)
Xie G,Roshandel D,Sherva R,Monach PA,Lu EY,Kung T,Carrington K,Zhang SS,Pulit SL,Ripke S,Carette S,Dellaripa PF,Edberg JC,Hoffman GS,Khalidi N,Langford CA,Mahr AD,St Clair EW,Seo P,Specks U,Spiera RF,Stone JH,Ytterberg SR,Raychaudhuri S,de Bakker PI,Farrer LA,Amos CI,Merkel PA,Siminovitch KA

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection.
Molecular human reproduction16(10)770-7(2010 Oct)
Ucisik-Akkaya E,Davis CF,Do TN,Morrison BA,Stemmer SM,Amadio WJ,Dorak MT

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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HSD17B8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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