FA2H cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FA2H
Entrez Gene ID	79152
Full Name	fatty acid 2-hydroxylase
Synonyms	FAAH,FAH1,FAXDC1,SCS7,SPG35
General protein information	Preferred Names fatty acid 2-hydroxylase Names fatty acid 2-hydroxylase fatty acid alpha-hydroxylase fatty acid hydroxylase domain containing 1 spastic paraplegia 35 (autosomal recessive)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.1
Summary	This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010].
Disorder MIM:	611026
Disorder Html:	Spastic paraplegia 35, autosomal recessive, 612319 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011523319.2	XP_011521621.1	fatty acid 2-hydroxylase isoform X2
NM_024306.4	NP_077282.3	fatty acid 2-hydroxylase
NM_024306.5	NP_077282.3	fatty acid 2-hydroxylase
XM_011523317.2	XP_011521619.1	fatty acid 2-hydroxylase isoform X1
XM_011523317.3	XP_011521619.1	fatty acid 2-hydroxylase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FA2H	XP_511106.3
	FA2H	XP_001108607.1
Mus musculus (house mouse)	Fa2h	NP_835187.2
Neurospora crassa	NCU03492	XP_955879.1
Saccharomyces cerevisiae (baker's yeast)	SCS7	NP_013999.1
Kluyveromyces lactis	KLLA0D01639g	XP_453142.1
Arabidopsis thaliana (thale cress)	FAH1	NP_181023.1
Xenopus tropicalis (tropical clawed frog)	fa2h	NP_001025677.1
Canis lupus familiaris (dog)	FA2H	XP_853014.1
Bos taurus (cattle)	FA2H	NP_001179384.1
Oryza sativa (rice)	Os03g0780800	NP_001051453.1
Oryza sativa (rice)	Os12g0628400	NP_001067329.1
Homo sapiens (human)	FA2H	NP_077282.3
Rattus norvegicus (Norway rat)	Fa2h	NP_001129055.1
Eremothecium gossypii	AGOS_AAL183W	NP_982359.1
Magnaporthe oryzae (rice blast fungus)	MGG_03920	XP_003719946.1
Gallus gallus (chicken)	FA2H	XP_414053.4
Drosophila melanogaster (fruit fly)	fa2h	NP_610279.3
Caenorhabditis elegans (roundworm)	CELE_C25A1.5	NP_492678.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in FA2H in three Arab families with a clinical spectrum of neurodegeneration and hereditary spastic paraparesis.
Zaki MS, Selim L, Mansour L, Mahmoud IG, Fenstermaker AG, Gabriel SB, Gleeson JG
Clinical genetics88(1)95-7(2015 Jul)

Results of a "GWAS plus:" general cognitive ability is substantially heritable and massively polygenic.
Kirkpatrick RM, McGue M, Iacono WG, Miller MB, Basu S
PloS one9(11)e112390(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Novel mutations in FA2H in Arab patients with a clinical spectrum of neurodegeneration and hereditary spastic paraparesis were identified.
Title: Mutations in FA2H in three Arab families with a clinical spectrum of neurodegeneration and hereditary spastic paraparesis.

Three novel mutations in Chinese patients significantly reduce FA2H enzyme activity leading to hereditary spastic paraplegia.
Title: SPG35 contributes to the second common subtype of AR-HSP in China: frequency analysis and functional characterization of FA2H gene mutations.

Induced levels of PPARalpha may be involved in the Delta(9)-THC up-regulation of FA2H in MDA-MB-231 cells.
Title: ?(9)-THC modulation of fatty acid 2-hydroxylase (FA2H) gene expression: possible involvement of induced levels of PPAR? in MDA-MB-231 breast cancer cells.

One heterozygous deletion within 16q22.3-q23.1 including FA2H was observed in two siblings who share symptoms of autism and severe cognitive impairment, axial T2-FLAIR weighted MRI posterior periventricular white matter lesions.
Title: Heterozygous FA2H mutations in autism spectrum disorders.

Identification of a novel triple heterozygous mutations in FA2H gene (c.968C>A; c.976G>A; c.688G>A) in a Chinese family with Hereditary Spastic Paraplegia Type 35.
Title: A rare family with Hereditary Spastic Paraplegia Type 35 due to novel FA2H mutations: a case report with literature review.

The following FA2H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FA2H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26599	NM_024306.5 Latest version!	Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu71804	XM_011523319.2 Latest version!	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu71802	XM_011523317.3 Latest version!	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu26599	NM_024306.4	Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu71802	XM_011523317.2	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FA2H ( NM_024306.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077282.3 Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.

Price & Availability↑

CloneID	OHu26599
Clone ID Related Accession (Same CDS sequence)	NM_024306.4 , NM_024306.5
Accession Version	NM_024306.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	fatty acid 2-hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA890369.1, BC017049.1 and AA857184.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2010 this sequence version replaced NM_024306.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK058016.1, BC017049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077282.3
CDS	77..1195
Translation	MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHSANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHTLTPEKPHLKTQ

Target ORF information:

RefSeq Version	NM_024306.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024306.4

ORF Insert Sequence:

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FA2H ( NM_024306.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_077282.3 Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.

Price & Availability↑

CloneID	OHu26599
Clone ID Related Accession (Same CDS sequence)	NM_024306.4 , NM_024306.5
Accession Version	NM_024306.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	fatty acid 2-hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA890369.1 and BC017049.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024306.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.205016.1, AK058016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000219368.8/ ENSP00000219368.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077282.3
CDS	66..1184
Translation	MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHSANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHTLTPEKPHLKTQ

Target ORF information:

RefSeq Version	NM_024306.5
Organism	Homo sapiens(human)
Definition	Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024306.5

ORF Insert Sequence:

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FA2H ( XM_011523319.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521621.1 Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu71804
Clone ID Related Accession (Same CDS sequence)	XM_011523319.2
Accession Version	XM_011523319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fatty acid 2-hydroxylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGTG TTCGCCTGTG GAATTCAAGG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521621.1
CDS	16..894
Translation	MDGVRLWNSRGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHTLTPEKPHLKTQ

Target ORF information:

RefSeq Version	XM_011523319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523319.2

ORF Insert Sequence:

ATGGATGGTG TTCGCCTGTG GAATTCAAGG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG 
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG 
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT 
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC 
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC 
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FA2H ( XM_011523317.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521619.1 Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71802
Clone ID Related Accession (Same CDS sequence)	XM_011523317.2 , XM_011523317.3
Accession Version	XM_011523317.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	fatty acid 2-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG 
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT 
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT 
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC 
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG 
CATAAATCAG AGAAAATATG GCAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521619.1
CDS	69..1178
Translation	MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHSANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKSRRLRPRRTKELAPGHTWHSQDVVPHSGDSRVCALNHPSPEALQGLHDSPRSWPEKIGLPKGSYLPCTPRCFSVPALCCPWPIPGCSPARLHTLFSAGHKSEKIWQI

Target ORF information:

RefSeq Version	XM_011523317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523317.2

ORF Insert Sequence:

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG 
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT 
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT 
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC 
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG 
CATAAATCAG AGAAAATATG GCAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FA2H ( XM_011523317.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521619.1 Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.

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CloneID	OHu71802
Clone ID Related Accession (Same CDS sequence)	XM_011523317.2 , XM_011523317.3
Accession Version	XM_011523317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fatty acid 2-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG 
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT 
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT 
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC 
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG 
CATAAATCAG AGAAAATATG GCAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521619.1
CDS	77..1186
Translation	MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHSANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKSRRLRPRRTKELAPGHTWHSQDVVPHSGDSRVCALNHPSPEALQGLHDSPRSWPEKIGLPKGSYLPCTPRCFSVPALCCPWPIPGCSPARLHTLFSAGHKSEKIWQI

Target ORF information:

RefSeq Version	XM_011523317.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523317.3

ORF Insert Sequence:

ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG 
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC 
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC 
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC 
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG 
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC 
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG 
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG 
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG 
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG 
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC 
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG 
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT 
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT 
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC 
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG 
CATAAATCAG AGAAAATATG GCAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in FA2H in three Arab families with a clinical spectrum of neurodegeneration and hereditary spastic paraparesis.
Clinical genetics88(1)95-7(2015 Jul)
Zaki MS,Selim L,Mansour L,Mahmoud IG,Fenstermaker AG,Gabriel SB,Gleeson JG

Results of a "GWAS plus:" general cognitive ability is substantially heritable and massively polygenic.
PloS one9(11)e112390(2014)
Kirkpatrick RM,McGue M,Iacono WG,Miller MB,Basu S

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FA2H cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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