FASTKD3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FASTKD3
Entrez Gene ID	79072
Full Name	FAST kinase domains 3
General protein information	Preferred Names FAST kinase domains 3 Names FAST kinase domain-containing protein 3, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p15.31
Summary	This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013].
Disorder MIM:	617530

mRNA and Protein(s)

mRNA	Protein	Name
NM_024091.3	NP_076996.2	FAST kinase domain-containing protein 3, mitochondrial
XM_006714498.1	XP_006714561.1	FAST kinase domain-containing protein 3, mitochondrial isoform X1
NM_024091.4	NP_076996.2	FAST kinase domain-containing protein 3, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FASTKD3	XP_517625.3
Rattus norvegicus (Norway rat)	Fastkd3	NP_001076043.1
Xenopus tropicalis (tropical clawed frog)	fastkd3	NP_001135619.2
Canis lupus familiaris (dog)	FASTKD3	XP_545176.4
Danio rerio (zebrafish)	fastkd3	XP_001338251.1
Bos taurus (cattle)	FASTKD3	NP_001019699.2
Gallus gallus (chicken)	FASTKD3	XP_419017.3
Homo sapiens (human)	FASTKD3	NP_076996.2
Mus musculus (house mouse)	Fastkd3	NP_081399.3

Gene Ontology
Bibliography

Related articles in PubMed

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Jourdain AA, Popow J, de la Fuente MA, Martinou JC, Anderson P, Simarro M
Nucleic acids research45(19)10941-10947(2017 Nov)

Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.
Boehm E, Zornoza M, Jourdain AA, Delmiro Magdalena A, Garc?a-Consuegra I, Torres Merino R, Ordu?a A, Mart?n MA, Martinou JC, De la Fuente MA, Simarro M
The Journal of biological chemistry291(50)25877-25887(2016 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Simarro M, Gimenez-Cassina A, Kedersha N, Lazaro JB, Adelmant GO, Marto JA, Rhee K, Tisdale S, Danial N, Benarafa C, Ordu?a A, Anderson P
Biochemical and biophysical research communications401(3)440-6(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Despite the fact that proteins of the FASTK family FASTKD1-5 share the same domains, they exhibit various-sometimes opposing-functions in almost all steps of mitochondrial RNA metabolism.
Title: The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.

FASTKD3 is required for efficient COX1 mRNA translation without altering mRNA levels, which results in a decrease in the steady-state levels of COX1 protein.
Title: Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

these results introduce FASTKD3 as an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration.
Title: Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following FASTKD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FASTKD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19265	NM_024091.4 Latest version!	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu19265	XM_006714498.1 Latest version!	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu19265	NM_024091.3	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FASTKD3 ( NM_024091.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_076996.2 Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19265
Clone ID Related Accession (Same CDS sequence)	NM_024091.3 , XM_006714498.1 , NM_024091.4
Accession Version	NM_024091.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 3, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA297400.1, AK026927.1, BQ921348.1, BP317791.1 and BC001295.1. This sequence is a reference standard in the RefSeqGene project. On Aug 19, 2010 this sequence version replaced NM_024091.2. Transcript Variant: This variant (1) is the longest transcript and encodes the protein. ##Evidence-Data-START## Transcript exon combination :: AK026927.1, SRR1660809.18992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20869947 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076996.2
CDS	173..2161
Translation	MALITLRKNLYRLSDFQMHRALAALKNKPLNHVHKVVKERLCPWLCSRQPEPFGVKFHHAHCKKFHSKNGNDLHPLGGPVFSQVSDCDRLEQNVKNEESQMFYRRLSNLTSSEEVLSFISTMETLPDTMAAGALQRICEVEKKDGDQGLPKEILENSIFQALCFQFEKEPSQLSNTSLVTALQALILLHVDPQSSLLLNLVAECQNRLRKGGMEVRNLCILGESLITLHSSGCVTLELIINQLQGEKLETFTPEDIVALYRILQACTEKVDEHQTFLNKINNFSLSIVSNLSPKLISQMLTALVVLDQSQAFPLIIKLGKYVVRHVPHFTNEELRRVLEAFIYFGHHDTFFTKALEHRVAAVCLTLDPEVVCRVMEYCSRELILSKPILNAVAETFVCQTEKFSPRQISALMEPFGKLNYLPPNASALFRKLENVLFTHFNYFPPKSLLKLLHSCSLNECHPVNFLAKIFKPLFLQRLQGKESHLDTLSRAQLTQLFLASVLECPFYKGPKLLPKYQVKSFLTPCCSLETPVDSQLYRYVKIGLTNLLGARLYFAPKVLTPYCYTIDVEIKLDEEGFVLPSTANEDIHKRIALCIDGPKRFCSNSKHLLGKEAIKQRHLQLLGYQVVQIPYHEIGMLKSRRELVEYLQRKLFSQNTVHWLQE

Target ORF information:

RefSeq Version	NM_024091.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024091.3

ORF Insert Sequence:

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASTKD3 ( NM_024091.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_076996.2 Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19265
Clone ID Related Accession (Same CDS sequence)	NM_024091.3 , XM_006714498.1 , NM_024091.4
Accession Version	NM_024091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 3, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA297400.1, AK026927.1, BQ921348.1, BP317791.1 and BC001295.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024091.3. Transcript Variant: This variant (1) is the longest transcript and encodes the protein. ##Evidence-Data-START## Transcript exon combination :: AK026927.1, SRR1660809.18992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20869947 MANE Ensembl match :: ENST00000264669.10/ ENSP00000264669.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076996.2
CDS	167..2155
Translation	MALITLRKNLYRLSDFQMHRALAALKNKPLNHVHKVVKERLCPWLCSRQPEPFGVKFHHAHCKKFHSKNGNDLHPLGGPVFSQVSDCDRLEQNVKNEESQMFYRRLSNLTSSEEVLSFISTMETLPDTMAAGALQRICEVEKKDGDQGLPKEILENSIFQALCFQFEKEPSQLSNTSLVTALQALILLHVDPQSSLLLNLVAECQNRLRKGGMEVRNLCILGESLITLHSSGCVTLELIINQLQGEKLETFTPEDIVALYRILQACTEKVDEHQTFLNKINNFSLSIVSNLSPKLISQMLTALVVLDQSQAFPLIIKLGKYVVRHVPHFTNEELRRVLEAFIYFGHHDTFFTKALEHRVAAVCLTLDPEVVCRVMEYCSRELILSKPILNAVAETFVCQTEKFSPRQISALMEPFGKLNYLPPNASALFRKLENVLFTHFNYFPPKSLLKLLHSCSLNECHPVNFLAKIFKPLFLQRLQGKESHLDTLSRAQLTQLFLASVLECPFYKGPKLLPKYQVKSFLTPCCSLETPVDSQLYRYVKIGLTNLLGARLYFAPKVLTPYCYTIDVEIKLDEEGFVLPSTANEDIHKRIALCIDGPKRFCSNSKHLLGKEAIKQRHLQLLGYQVVQIPYHEIGMLKSRRELVEYLQRKLFSQNTVHWLQE

Target ORF information:

RefSeq Version	NM_024091.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024091.4

ORF Insert Sequence:

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASTKD3 ( XM_006714498.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714561.1 Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant X1, mRNA.

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CloneID	OHu19265
Clone ID Related Accession (Same CDS sequence)	NM_024091.3 , XM_006714498.1 , NM_024091.4
Accession Version	XM_006714498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714561.1
CDS	84..2072
Translation	MALITLRKNLYRLSDFQMHRALAALKNKPLNHVHKVVKERLCPWLCSRQPEPFGVKFHHAHCKKFHSKNGNDLHPLGGPVFSQVSDCDRLEQNVKNEESQMFYRRLSNLTSSEEVLSFISTMETLPDTMAAGALQRICEVEKKDGDQGLPKEILENSIFQALCFQFEKEPSQLSNTSLVTALQALILLHVDPQSSLLLNLVAECQNRLRKGGMEVRNLCILGESLITLHSSGCVTLELIINQLQGEKLETFTPEDIVALYRILQACTEKVDEHQTFLNKINNFSLSIVSNLSPKLISQMLTALVVLDQSQAFPLIIKLGKYVVRHVPHFTNEELRRVLEAFIYFGHHDTFFTKALEHRVAAVCLTLDPEVVCRVMEYCSRELILSKPILNAVAETFVCQTEKFSPRQISALMEPFGKLNYLPPNASALFRKLENVLFTHFNYFPPKSLLKLLHSCSLNECHPVNFLAKIFKPLFLQRLQGKESHLDTLSRAQLTQLFLASVLECPFYKGPKLLPKYQVKSFLTPCCSLETPVDSQLYRYVKIGLTNLLGARLYFAPKVLTPYCYTIDVEIKLDEEGFVLPSTANEDIHKRIALCIDGPKRFCSNSKHLLGKEAIKQRHLQLLGYQVVQIPYHEIGMLKSRRELVEYLQRKLFSQNTVHWLQE

Target ORF information:

RefSeq Version	XM_006714498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 3 (FASTKD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714498.1

ORF Insert Sequence:

ATGGCATTAA TCACCTTGAG GAAGAACCTT TATCGTTTAT CTGATTTTCA GATGCATAGA 
GCTCTGGCTG CTTTAAAAAA TAAACCTCTA AATCATGTTC ACAAGGTAGT CAAGGAGCGT 
CTGTGCCCTT GGTTGTGTTC ACGACAACCT GAGCCTTTCG GGGTCAAATT CCATCATGCC 
CATTGTAAAA AGTTTCATTC GAAAAATGGA AATGACCTTC ATCCACTCGG TGGACCAGTG 
TTCTCTCAAG TATCTGACTG CGACAGGCTT GAACAAAATG TTAAAAATGA GGAGAGTCAG 
ATGTTTTACA GGAGACTGAG CAACTTGACT TCATCAGAAG AAGTGCTAAG TTTTATAAGC 
ACGATGGAAA CCCTGCCTGA CACTATGGCA GCAGGAGCTT TACAACGAAT TTGTGAAGTG 
GAAAAAAAGG ATGGTGATCA AGGGCTGCCA AAAGAAATAC TGGAGAATAG CATCTTTCAA 
GCTTTATGCT TTCAGTTTGA AAAGGAGCCC TCACAGCTGT CAAACACTAG TTTAGTGACT 
GCTTTGCAAG CTCTGATTCT GTTGCATGTG GATCCTCAAA GTAGCCTGTT GCTGAACCTG 
GTGGCAGAAT GCCAAAATCG TCTCAGAAAA GGTGGCATGG AAGTTCGCAA TCTTTGTATT 
CTTGGGGAAA GTCTGATTAC ACTGCACAGT TCAGGTTGTG TGACACTAGA ACTCATTATA 
AATCAACTTC AAGGTGAAAA ATTGGAAACA TTTACCCCGG AGGATATTGT GGCCCTTTAT 
AGAATCTTGC AGGCATGTAC TGAAAAAGTG GATGAACACC AAACATTTTT AAATAAGATA 
AACAACTTTT CCCTATCAAT AGTTTCCAAC CTGAGTCCTA AATTGATTAG CCAAATGCTC 
ACTGCCCTGG TGGTTCTTGA TCAAAGTCAA GCATTTCCTC TGATTATAAA ATTGGGCAAA 
TATGTCGTGA GGCATGTCCC ACATTTCACT AACGAGGAGC TTAGGAGAGT CTTGGAGGCG 
TTCATATATT TTGGGCACCA TGACACATTT TTTACAAAAG CCCTAGAGCA TCGTGTAGCT 
GCGGTGTGCC TCACGTTGGA TCCTGAAGTT GTCTGCAGGG TCATGGAGTA CTGCAGTAGA 
GAACTGATTC TTTCAAAACC CATCCTCAAT GCAGTGGCAG AAACTTTTGT TTGCCAAACA 
GAAAAATTTT CACCTCGTCA GATTTCTGCC TTAATGGAAC CATTTGGGAA ACTCAATTAT 
TTGCCACCAA ATGCCTCTGC TTTATTTAGA AAGCTGGAAA ACGTGCTATT CACTCATTTC 
AATTATTTTC CACCCAAATC ATTATTGAAA CTTCTTCATT CATGTTCACT TAATGAATGC 
CATCCAGTCA ACTTTCTGGC AAAAATATTC AAGCCTCTTT TCCTTCAACG GCTGCAAGGT 
AAAGAATCTC ATTTGGACAC ATTGAGTCGG GCACAACTGA CCCAACTTTT CTTAGCCTCA 
GTCCTGGAAT GCCCTTTCTA TAAGGGTCCA AAACTCCTTC CTAAATATCA AGTGAAGTCA 
TTTCTTACCC CATGCTGTTC CCTGGAGACC CCTGTGGATT CTCAGCTTTA TAGATATGTG 
AAGATTGGGC TGACTAACCT TTTAGGAGCA AGATTATATT TTGCTCCAAA AGTGTTGACA 
CCCTATTGTT ATACAATAGA TGTTGAAATT AAATTAGATG AAGAAGGATT TGTATTGCCA 
TCCACAGCTA ATGAAGATAT CCATAAAAGG ATAGCACTGT GTATTGATGG TCCAAAAAGG 
TTTTGCTCCA ATAGCAAACA CTTACTGGGG AAAGAAGCTA TTAAACAAAG ACACCTACAG 
TTACTCGGTT ATCAAGTTGT TCAGATCCCC TATCATGAGA TTGGGATGCT AAAATCAAGA 
CGTGAATTGG TGGAATATTT ACAAAGAAAA CTGTTTTCTC AAAACACTGT TCATTGGTTG 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Nucleic acids research45(19)10941-10947(2017 Nov)
Jourdain AA,Popow J,de la Fuente MA,Martinou JC,Anderson P,Simarro M

Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.
The Journal of biological chemistry291(50)25877-25887(2016 Dec)
Boehm E,Zornoza M,Jourdain AA,Delmiro Magdalena A,Garc?a-Consuegra I,Torres Merino R,Ordu?a A,Mart?n MA,Martinou JC,De la Fuente MA,Simarro M

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Biochemical and biophysical research communications401(3)440-6(2010 Oct)
Simarro M,Gimenez-Cassina A,Kedersha N,Lazaro JB,Adelmant GO,Marto JA,Rhee K,Tisdale S,Danial N,Benarafa C,Ordu?a A,Anderson P

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FASTKD3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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