MRPL57 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL57
Entrez Gene ID	78988
Full Name	mitochondrial ribosomal protein L57
Synonyms	MRP63,bMRP63
General protein information	Preferred Names mitochondrial ribosomal protein L57 Names ribosomal protein 63, mitochondrial hMRP63 mitochondrial large ribosomal subunit protein mL63 mitochondrial ribosomal protein 63 mitochondrial ribosomal protein bMRP63
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.11
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein which belongs to an undetermined ribosomal subunit and which seems to be specific to animal mitoribosomes. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 3p, 5q, 8q, 14q, and Y. [provided by RefSeq, Jul 2008].
Disorder MIM:	611997

mRNA and Protein(s)

mRNA	Protein	Name
XM_017020740.1	XP_016876229.1	ribosomal protein 63, mitochondrial isoform X1
NM_024026.5	NP_076931.1	ribosomal protein 63, mitochondrial
NM_024026.4	NP_076931.1	ribosomal protein 63, mitochondrial
XM_017020740.2	XP_016876229.1	ribosomal protein 63, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mrp63	NP_080677.1
Xenopus tropicalis (tropical clawed frog)	LOC100491945	XP_002934730.2
Canis lupus familiaris (dog)	MRP63	XP_851725.1
Danio rerio (zebrafish)	mrpl57	NP_957069.1
Homo sapiens (human)	MRPL57	NP_076931.1
Bos taurus (cattle)	MRP63	NP_001029959.1
Pan troglodytes (chimpanzee)	MRP63	XP_003314120.1
	MRP63	XP_001084755.1
Rattus norvegicus (Norway rat)	Mrp63	NP_001103119.1
Gallus gallus (chicken)	MRP63	XP_003640629.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome.
Koc EC, Cimen H, Kumcuoglu B, Abu N, Akpinar G, Haque ME, Spremulli LL, Koc H
Frontiers in physiology4183(2013)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.
Kenmochi N, Suzuki T, Uechi T, Magoori M, Kuniba M, Higa S, Watanabe K, Tanaka T
Genomics77(1-2)65-70(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL57 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL57 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00012	NM_024026.5 Latest version!	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00012	XM_017020740.2 Latest version!	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu00012	NM_024026.4	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00012	XM_017020740.1	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL57 ( NM_024026.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_076931.1 Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.

Price & Availability↑

CloneID	OHu00012
Clone ID Related Accession (Same CDS sequence)	XM_017020740.1 , NM_024026.5 , NM_024026.4 , XM_017020740.2
Accession Version	NM_024026.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	ribosomal protein 63, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158032.32. On Nov 23, 2018 this sequence version replaced NM_024026.4. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.663564.1, SRR1660807.250558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000309594.5/ ENSP00000310726.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076931.1
CDS	66..374
Translation	MFLTALLWRGRIPGRQWIGKHRRPRFVSLRAKQNMIRRLEIEAENHYWLSMPYMTREQERGHAAVRRREAFEAIKAAATSKFPPHRFIADQLDHLNVTKKWS

Target ORF information:

RefSeq Version	NM_024026.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024026.5

ORF Insert Sequence:

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL57 ( NM_024026.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_076931.1 Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.

Price & Availability↑

CloneID	OHu00012
Clone ID Related Accession (Same CDS sequence)	XM_017020740.1 , NM_024026.5 , NM_024026.4 , XM_017020740.2
Accession Version	NM_024026.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ribosomal protein 63, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW445203.1, BG773624.1, BI601227.1, BC023616.2, BC068492.1 and CF890784.1. On Apr 14, 2004 this sequence version replaced NM_024026.3. ##Evidence-Data-START## Transcript exon combination :: BC068492.1, SRR1660803.94366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076931.1
CDS	491..799
Translation	MFLTALLWRGRIPGRQWIGKHRRPRFVSLRAKQNMIRRLEIEAENHYWLSMPYMTREQERGHAAVRRREAFEAIKAAATSKFPPHRFIADQLDHLNVTKKWS

Target ORF information:

RefSeq Version	NM_024026.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024026.4

ORF Insert Sequence:

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL57 ( XM_017020740.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876229.1 Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu00012
Clone ID Related Accession (Same CDS sequence)	XM_017020740.1 , NM_024026.5 , NM_024026.4 , XM_017020740.2
Accession Version	XM_017020740.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ribosomal protein 63, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876229.1
CDS	125..433
Translation	MFLTALLWRGRIPGRQWIGKHRRPRFVSLRAKQNMIRRLEIEAENHYWLSMPYMTREQERGHAAVRRREAFEAIKAAATSKFPPHRFIADQLDHLNVTKKWS

Target ORF information:

RefSeq Version	XM_017020740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020740.1

ORF Insert Sequence:

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL57 ( XM_017020740.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876229.1 Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu00012
Clone ID Related Accession (Same CDS sequence)	XM_017020740.1 , NM_024026.5 , NM_024026.4 , XM_017020740.2
Accession Version	XM_017020740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ribosomal protein 63, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876229.1
CDS	125..433
Translation	MFLTALLWRGRIPGRQWIGKHRRPRFVSLRAKQNMIRRLEIEAENHYWLSMPYMTREQERGHAAVRRREAFEAIKAAATSKFPPHRFIADQLDHLNVTKKWS

Target ORF information:

RefSeq Version	XM_017020740.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L57 (MRPL57), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020740.2

ORF Insert Sequence:

ATGTTCCTGA CTGCGCTCCT CTGGCGCGGC CGCATTCCCG GCCGTCAGTG GATCGGGAAG 
CACCGGCGGC CGCGGTTCGT GTCGTTGCGC GCCAAGCAGA ACATGATCCG CCGCCTGGAG 
ATCGAGGCGG AGAACCATTA CTGGCTGAGC ATGCCCTACA TGACCCGGGA GCAGGAGCGC 
GGCCACGCCG CGGTGCGCAG GAGGGAGGCC TTCGAGGCCA TAAAGGCGGC CGCCACTTCC 
AAGTTCCCCC CGCATAGATT CATTGCGGAC CAGCTCGACC ATCTCAATGT CACCAAGAAA 
TGGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome.
Frontiers in physiology4183(2013)
Koc EC,Cimen H,Kumcuoglu B,Abu N,Akpinar G,Haque ME,Spremulli LL,Koc H

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.
Genomics77(1-2)65-70(2001 Sep)
Kenmochi N,Suzuki T,Uechi T,Magoori M,Kuniba M,Higa S,Watanabe K,Tanaka T

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MRPL57 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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