CACNA1S cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1S
Entrez Gene ID	779
Full Name	calcium voltage-gated channel subunit alpha1 S
Synonyms	CACNL1A3,CCHL1A3,Cav1.1,HOKPP,HOKPP1,MHS5,TTPP1,hypoPP
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 S Names voltage-dependent L-type calcium channel subunit alpha-1S calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis) calcium channel, voltage-dependent, L type, alpha 1S subunit dihydropyridine receptor alpha 1 subunit dihydropyridine-sensitive L-type calcium channel alpha-1 subunit voltage-gated calcium channel subunit alpha Cav1.1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility. [provided by RefSeq, Jul 2008].
Disorder MIM:	114208
Disorder Html:	Hypokalemic periodic paralysis, type 1, 170400 (3); {Malignant hyperthermia susceptibility 5}, 601887 (3); {Thyrotoxic periodic paralysis, susceptibility to, 1}, 188580 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000069.3	NP_000060.2	voltage-dependent L-type calcium channel subunit alpha-1S
NM_000069.2	NP_000060.2	voltage-dependent L-type calcium channel subunit alpha-1S
XM_005245478.3	XP_005245535.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1

Pathways from BioSystems

Homologies

	CACNA1S	XP_002802046.1
Rattus norvegicus (Norway rat)	Cacna1s	NP_446325.1
Homo sapiens (human)	CACNA1S	NP_000060.2
Pan troglodytes (chimpanzee)	CACNA1S	XP_525018.3
Canis lupus familiaris (dog)	CACNA1S	XP_848685.3
Bos taurus (cattle)	CACNA1S	XP_002694288.2
Mus musculus (house mouse)	Cacna1s	NP_001074492.1
Danio rerio (zebrafish)	cacna1sa	NP_001139622.1
Gallus gallus (chicken)	CACNA1S	XP_419259.3
Danio rerio (zebrafish)	cacna1sb	NP_999891.1

Gene Ontology
Bibliography

Related articles in PubMed

Na leak with gating pore properties in hypokalemic periodic paralysis V876E mutant muscle Ca channel.
Fuster C, Perrot J, Berthier C, Jacquemond V, Charnet P, Allard B
The Journal of general physiology149(12)1139-1148(2017 Dec)

Mechanisms Responsible for ?-Pore Currents in Ca
Monteleone S, Lieb A, Pinggera A, Negro G, Fuchs JE, Hofer F, Striessnig J, Tuluc P, Liedl KR
Biophysical journal113(7)1485-1495(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study used structure modeling and MD simulations to predict pathogenic omega-currents in CaV1.1 and CaV1.3 Ca(2+) channels bearing several S4 charge mutations: omega-currents conducted in resting state, but not during voltage-sensing domain activation. Mechanism responsible depends on the number of charges in S4, the position of the mutated S4 charge and countercharges, and the nature of the replacing amino acid.
Title: Mechanisms Responsible for ?-Pore Currents in Ca

Study identified by exome sequencing both recessive and dominant CACNA1S mutations as a cause of a congenital myopathy characterized by peculiar morphological hallmarks in a cohort of 11 patients from 7 families.
Title: Dihydropyridine receptor (DHPR, CACNA1S) congenital myopathy.

These results provide new insights into the role of muscle-specific proteins on the structural arrangement of alpha1S intracellular loops and point to a new conformational effect of the beta1a subunit in supporting skeletal muscle excitation-contraction coupling.
Title: Fluorescence Resonance Energy Transfer-based Structural Analysis of the Dihydropyridine Receptor ?1S Subunit Reveals Conformational Differences Induced by Binding of the ?1a Subunit.

whole-exome next-generation sequencing was used to identify a mutation in the CACNA1S gene, R900S, which is a rare mutation associated with hypokalemic periodic paralysis; study provides further evidence for the phenotypic variation and pharmacogenomics of hypokalemic periodic paralysis
Title: The R900S mutation in CACNA1S associated with hypokalemic periodic paralysis.

CACNA1S and SCN4A mutations are relatively rare in patients with hypokalemic periodic paralysis
Title: Mutation analysis of CACNA1S and SCN4A in patients with hypokalemic periodic paralysis.

The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19719	NM_000069.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu34684	XM_005245478.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu19719	NM_000069.2	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1S ( NM_000069.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000060.2 Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.

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CloneID	OHu19719
Clone ID Related Accession (Same CDS sequence)	NM_000069.3 , NM_000069.2
Accession Version	NM_000069.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139159.10 and AL358473.10. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000069.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L33798.1, BC133671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000362061.4/ ENSP00000355192.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG TGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAAGGAGTGC GAACCCTCCT GTGGACGTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCTGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCT TGGTGGATGG GACCCAAATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGC TACTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCCTACTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCA 
GAGTCGGACT ATGCCCCAGG GGAGGAGTAC ACATGTGGCA CCAACTTTGC ATACTACTAC 
TTCATCAGCT TCTACATGCT CTGTGCCTTC CTGGTCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTACCT CACCCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC AGAGTATGAC CCAGAGGCTA AGGGGAGAAT CAAACACCTG 
GACGTGGTGA CCCTGCTGAG AAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CATCGGGTAG CTTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGACGGCACA 
GTCACCTTCA ATGCCACACT CTTTGCCCTG GTCCGCACGG CACTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAGGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTG 
GGGAAGTTCT ACGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAACGCCAA 
GAGGAGTATT ATGGCTATCG GCCCAAGAAG GACATTGTAC AGATCCAGGC AGGGCTGCGG 
ACCATTGAGG AAGAGGCAGC CCCCGAGATC TGTCGCACGG TCTCAGGAGA CCTGGCTGCT 
GAGGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAGGGAAT ATTCCGGAGG 
ACTGGAGGCC TGTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCC 
GTCATGGCCA ATCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GATGGAGTCA 
CCTGTCTTCT TGGAGGACTT CCCACAAGAT CCACGCACCA ACCCCCTGGC TCGTGCCAAT 
ACCAACAATG CCAACGCCAA TGTCGCCTAT GGCAACAGCA ACCATAGCAA CAGCCATGTG 
TTTTCCAGTG TCCACTATGA AAGGGAGTTC CCAGAAGAGA CAGAGACGCC TGCTACCAGA 
GGACGAGCCC TTGGCCAACC CTGCAGGGTC CTGGGACCCC ACAGCAAACC CTGTGTGGAG 
ATGCTGAAGG GACTGCTGAC CCAGAGGGCA ATGCCCAGAG GCCAGGCACC TCCTGCCCCC 
TGCCAGTGCC CCAGGGTGGA GTCCTCCATG CCTGAGGACA GAAAGAGCTC CACACCAGGG 
TCTCTTCATG AGGAGACACC CCACAGCAGG AGCACCAGGG AGAATACTTC CAGGTGCTCA 
GCACCAGCTA CAGCCCTGCT GATCCAAAAG GCTCTGGTTC GAGGGGGCCT GGGCACCTTG 
GCAGCTGATG CAAACTTCAT CATGGCAACA GGCCAGGCCC TGGCAGATGC CTGCCAAATG 
GAACCAGAGG AAGTGGAGAT CATGGCAACA GAGCTACTGA AAGGACGAGA GGCCCCAGAG 
GGCATGGCCA GCTCCCTGGG ATGCCTGAAC CTCGGGTCCT CCCTGGGCAG CCTCGACCAA 
CACCAGGGCT CCCAGGAGAC CCTTATTCCT CCAAGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000060.2
CDS	88..5709
Translation	MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSMPEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKGREAPEGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL

Target ORF information:

RefSeq Version	NM_000069.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000069.3

ORF Insert Sequence:

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG TGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAAGGAGTGC GAACCCTCCT GTGGACGTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCTGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCT TGGTGGATGG GACCCAAATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGC TACTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCCTACTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCA 
GAGTCGGACT ATGCCCCAGG GGAGGAGTAC ACATGTGGCA CCAACTTTGC ATACTACTAC 
TTCATCAGCT TCTACATGCT CTGTGCCTTC CTGGTCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTACCT CACCCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC AGAGTATGAC CCAGAGGCTA AGGGGAGAAT CAAACACCTG 
GACGTGGTGA CCCTGCTGAG AAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CATCGGGTAG CTTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGACGGCACA 
GTCACCTTCA ATGCCACACT CTTTGCCCTG GTCCGCACGG CACTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAGGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTG 
GGGAAGTTCT ACGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAACGCCAA 
GAGGAGTATT ATGGCTATCG GCCCAAGAAG GACATTGTAC AGATCCAGGC AGGGCTGCGG 
ACCATTGAGG AAGAGGCAGC CCCCGAGATC TGTCGCACGG TCTCAGGAGA CCTGGCTGCT 
GAGGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAGGGAAT ATTCCGGAGG 
ACTGGAGGCC TGTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCC 
GTCATGGCCA ATCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GATGGAGTCA 
CCTGTCTTCT TGGAGGACTT CCCACAAGAT CCACGCACCA ACCCCCTGGC TCGTGCCAAT 
ACCAACAATG CCAACGCCAA TGTCGCCTAT GGCAACAGCA ACCATAGCAA CAGCCATGTG 
TTTTCCAGTG TCCACTATGA AAGGGAGTTC CCAGAAGAGA CAGAGACGCC TGCTACCAGA 
GGACGAGCCC TTGGCCAACC CTGCAGGGTC CTGGGACCCC ACAGCAAACC CTGTGTGGAG 
ATGCTGAAGG GACTGCTGAC CCAGAGGGCA ATGCCCAGAG GCCAGGCACC TCCTGCCCCC 
TGCCAGTGCC CCAGGGTGGA GTCCTCCATG CCTGAGGACA GAAAGAGCTC CACACCAGGG 
TCTCTTCATG AGGAGACACC CCACAGCAGG AGCACCAGGG AGAATACTTC CAGGTGCTCA 
GCACCAGCTA CAGCCCTGCT GATCCAAAAG GCTCTGGTTC GAGGGGGCCT GGGCACCTTG 
GCAGCTGATG CAAACTTCAT CATGGCAACA GGCCAGGCCC TGGCAGATGC CTGCCAAATG 
GAACCAGAGG AAGTGGAGAT CATGGCAACA GAGCTACTGA AAGGACGAGA GGCCCCAGAG 
GGCATGGCCA GCTCCCTGGG ATGCCTGAAC CTCGGGTCCT CCCTGGGCAG CCTCGACCAA 
CACCAGGGCT CCCAGGAGAC CCTTATTCCT CCAAGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( NM_000069.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000060.2 Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.

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CloneID	OHu19719
Clone ID Related Accession (Same CDS sequence)	NM_000069.3 , NM_000069.2
Accession Version	NM_000069.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139159.10 and AL358473.10. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2006 this sequence version replaced NM_000069.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L33798.1, BC133671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG TGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAAGGAGTGC GAACCCTCCT GTGGACGTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCTGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCT TGGTGGATGG GACCCAAATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGC TACTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCCTACTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCA 
GAGTCGGACT ATGCCCCAGG GGAGGAGTAC ACATGTGGCA CCAACTTTGC ATACTACTAC 
TTCATCAGCT TCTACATGCT CTGTGCCTTC CTGGTCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTACCT CACCCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC AGAGTATGAC CCAGAGGCTA AGGGGAGAAT CAAACACCTG 
GACGTGGTGA CCCTGCTGAG AAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CATCGGGTAG CTTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGACGGCACA 
GTCACCTTCA ATGCCACACT CTTTGCCCTG GTCCGCACGG CACTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAGGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTG 
GGGAAGTTCT ACGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAACGCCAA 
GAGGAGTATT ATGGCTATCG GCCCAAGAAG GACATTGTAC AGATCCAGGC AGGGCTGCGG 
ACCATTGAGG AAGAGGCAGC CCCCGAGATC TGTCGCACGG TCTCAGGAGA CCTGGCTGCT 
GAGGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAGGGAAT ATTCCGGAGG 
ACTGGAGGCC TGTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCC 
GTCATGGCCA ATCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GATGGAGTCA 
CCTGTCTTCT TGGAGGACTT CCCACAAGAT CCACGCACCA ACCCCCTGGC TCGTGCCAAT 
ACCAACAATG CCAACGCCAA TGTCGCCTAT GGCAACAGCA ACCATAGCAA CAGCCATGTG 
TTTTCCAGTG TCCACTATGA AAGGGAGTTC CCAGAAGAGA CAGAGACGCC TGCTACCAGA 
GGACGAGCCC TTGGCCAACC CTGCAGGGTC CTGGGACCCC ACAGCAAACC CTGTGTGGAG 
ATGCTGAAGG GACTGCTGAC CCAGAGGGCA ATGCCCAGAG GCCAGGCACC TCCTGCCCCC 
TGCCAGTGCC CCAGGGTGGA GTCCTCCATG CCTGAGGACA GAAAGAGCTC CACACCAGGG 
TCTCTTCATG AGGAGACACC CCACAGCAGG AGCACCAGGG AGAATACTTC CAGGTGCTCA 
GCACCAGCTA CAGCCCTGCT GATCCAAAAG GCTCTGGTTC GAGGGGGCCT GGGCACCTTG 
GCAGCTGATG CAAACTTCAT CATGGCAACA GGCCAGGCCC TGGCAGATGC CTGCCAAATG 
GAACCAGAGG AAGTGGAGAT CATGGCAACA GAGCTACTGA AAGGACGAGA GGCCCCAGAG 
GGCATGGCCA GCTCCCTGGG ATGCCTGAAC CTCGGGTCCT CCCTGGGCAG CCTCGACCAA 
CACCAGGGCT CCCAGGAGAC CCTTATTCCT CCAAGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000060.2
CDS	228..5849
Translation	MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSMPEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKGREAPEGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL

Target ORF information:

RefSeq Version	NM_000069.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000069.2

ORF Insert Sequence:

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG TGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAAGGAGTGC GAACCCTCCT GTGGACGTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCTGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCT TGGTGGATGG GACCCAAATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGC TACTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCCTACTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCA 
GAGTCGGACT ATGCCCCAGG GGAGGAGTAC ACATGTGGCA CCAACTTTGC ATACTACTAC 
TTCATCAGCT TCTACATGCT CTGTGCCTTC CTGGTCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAATT TTGACTACCT CACCCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC AGAGTATGAC CCAGAGGCTA AGGGGAGAAT CAAACACCTG 
GACGTGGTGA CCCTGCTGAG AAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CATCGGGTAG CTTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGACGGCACA 
GTCACCTTCA ATGCCACACT CTTTGCCCTG GTCCGCACGG CACTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAGGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTG 
GGGAAGTTCT ACGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAACGCCAA 
GAGGAGTATT ATGGCTATCG GCCCAAGAAG GACATTGTAC AGATCCAGGC AGGGCTGCGG 
ACCATTGAGG AAGAGGCAGC CCCCGAGATC TGTCGCACGG TCTCAGGAGA CCTGGCTGCT 
GAGGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAGGGAAT ATTCCGGAGG 
ACTGGAGGCC TGTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCC 
GTCATGGCCA ATCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GATGGAGTCA 
CCTGTCTTCT TGGAGGACTT CCCACAAGAT CCACGCACCA ACCCCCTGGC TCGTGCCAAT 
ACCAACAATG CCAACGCCAA TGTCGCCTAT GGCAACAGCA ACCATAGCAA CAGCCATGTG 
TTTTCCAGTG TCCACTATGA AAGGGAGTTC CCAGAAGAGA CAGAGACGCC TGCTACCAGA 
GGACGAGCCC TTGGCCAACC CTGCAGGGTC CTGGGACCCC ACAGCAAACC CTGTGTGGAG 
ATGCTGAAGG GACTGCTGAC CCAGAGGGCA ATGCCCAGAG GCCAGGCACC TCCTGCCCCC 
TGCCAGTGCC CCAGGGTGGA GTCCTCCATG CCTGAGGACA GAAAGAGCTC CACACCAGGG 
TCTCTTCATG AGGAGACACC CCACAGCAGG AGCACCAGGG AGAATACTTC CAGGTGCTCA 
GCACCAGCTA CAGCCCTGCT GATCCAAAAG GCTCTGGTTC GAGGGGGCCT GGGCACCTTG 
GCAGCTGATG CAAACTTCAT CATGGCAACA GGCCAGGCCC TGGCAGATGC CTGCCAAATG 
GAACCAGAGG AAGTGGAGAT CATGGCAACA GAGCTACTGA AAGGACGAGA GGCCCCAGAG 
GGCATGGCCA GCTCCCTGGG ATGCCTGAAC CTCGGGTCCT CCCTGGGCAG CCTCGACCAA 
CACCAGGGCT CCCAGGAGAC CCTTATTCCT CCAAGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_005245478.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245535.1 Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

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CloneID	OHu34684
Clone ID Related Accession (Same CDS sequence)	XM_005245478.3
Accession Version	XM_005245478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACG ACCCAGATGA GAGTGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAAGGAG TGCGAACCCT CCTGTGGACG 
TTCATCAAGT CCTTCCAGGC CCTACCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCTG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCTTGGTGGA TGGGACCCAA 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGCTACTGCT CTTCAGGTGT 
GCAACAGGTG AGGCCTGGCA GGAGATCCTA CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCAGAGTCGG ACTATGCCCC AGGGGAGGAG TACACATGTG GCACCAACTT TGCATACTAC 
TACTTCATCA GCTTCTACAT GCTCTGTGCC TTCCTGGTCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ATTTTGACTA CCTCACCCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATCTG GGCAGAGTAT GACCCAGAGG CTAAGGGGAG AATCAAACAC 
CTGGACGTGG TGACCCTGCT GAGAAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACATCGGG TAGCTTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGACGGC 
ACAGTCACCT TCAATGCCAC ACTCTTTGCC CTGGTCCGCA CGGCACTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAGGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTGGGGAAGT TCTACGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAACGC 
CAAGAGGAGT ATTATGGCTA TCGGCCCAAG AAGGACATTG TACAGATCCA GGCAGGGCTG 
CGGACCATTG AGGAAGAGGC AGCCCCCGAG ATCTGTCGCA CGGTCTCAGG AGACCTGGCT 
GCTGAGGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAGGG AATATTCCGG 
AGGACTGGAG GCCTGTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCCGTCATGG CCAATCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGATGGAG 
TCACCTGTCT TCTTGGAGGA CTTCCCACAA GATCCACGCA CCAACCCCCT GGCTCGTGCC 
AATACCAACA ATGCCAACGC CAATGTCGCC TATGGCAACA GCAACCATAG CAACAGCCAT 
GTGTTTTCCA GTGTCCACTA TGAAAGGGAG TTCCCAGAAG AGACAGAGAC GCCTGCTACC 
AGAGGACGAG CCCTTGGCCA ACCCTGCAGG GTCCTGGGAC CCCACAGCAA ACCCTGTGTG 
GAGATGCTGA AGGGACTGCT GACCCAGAGG GCAATGCCCA GAGGCCAGGC ACCTCCTGCC 
CCCTGCCAGT GCCCCAGGGT GGAGTCCTCC ATGCCTGAGG ACAGAAAGAG CTCCACACCA 
GGGTCTCTTC ATGAGGAGAC ACCCCACAGC AGGAGCACCA GGGAGAATAC TTCCAGGTGC 
TCAGCACCAG CTACAGCCCT GCTGATCCAA AAGGCTCTGG TTCGAGGGGG CCTGGGCACC 
TTGGCAGCTG ATGCAAACTT CATCATGGCA ACAGGCCAGG CCCTGGCAGA TGCCTGCCAA 
ATGGAACCAG AGGAAGTGGA GATCATGGCA ACAGAGCTAC TGAAAGGACG AGAGGCCCCA 
GAGGGCATGG CCAGCTCCCT GGGATGCCTG AACCTCGGGT CCTCCCTGGG CAGCCTCGAC 
CAACACCAGG GCTCCCAGGA GACCCTTATT CCTCCAAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245535.1
CDS	115..5679
Translation	MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSMPEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKGREAPEGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL

Target ORF information:

RefSeq Version	XM_005245478.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245478.3

ORF Insert Sequence:

ATGGAGCCAT CCTCACCCCA GGATGAAGGC CTGAGGAAGA AACAGCCCAA GAAGCCAGTT 
CCTGAGATTC TGCCAAGGCC ACCCCGGGCC TTGTTCTGCC TGACCCTGGA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT TGTAGAATGG AAGCCCTTCG AGACGATCAT CTTGCTCACC 
ATCTTTGCCA ATTGTGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAAGA TGACAACAAC 
TCTCTGAACC TCGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTCTT CTCGATTGAA 
GCCGCCATGA AGATCATTGC CTACGGCTTC TTATTCCACC AGGACGCTTA CCTGCGCAGT 
GGCTGGAATG TGCTGGACTT CACCATTGTC TTCCTGGGGG TCTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CCACACAGCC CCAATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTCAAGGCCC TCAGAGCCTT CCGAGTGCTC AGACCCCTCC GGCTGGTGTC GGGGGTGCCT 
AGCCTGCAGG TGGTCCTGAA CTCCATCTTC AAGGCCATGC TCCCCCTCTT TCACATCGCC 
CTGCTGGTCC TCTTTATGGT CATCATCTAT GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGT ACAGATATCG TGGCCACGGT GGAGAATGAA 
GAGCCATCGC CCTGCGCCAG GACGGGCTCA GGGCGCCGGT GCACCATCAA TGGCAGTGAG 
TGCCGGGGCG GCTGGCCAGG GCCCAACCAT GGCATCACCC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATTACC ATGGAGGGAT GGACTGACGT CCTTTACTGG 
GTCAATGATG CCATCGGGAA TGAGTGGCCC TGGATCTATT TTGTCACCCT CATTTTGCTG 
GGATCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAACTAGAT 
GAGGACCTTC GGGGCTACAT GAGCTGGATC ACGCAGGGCG AGGTCATGGA TGTTGAGGAC 
TTCAGAGAAG GAAAACTGTC TTTGGATGAA GGTGGCTCTG ACACAGAGAG CCTGTATGAA 
ATTGCAGGCT TGAACAAAAT CATCCAGTTC ATCCGACATT GGAGGCAGTG GAACCGCATC 
TTTCGCTGGA AGTGCCATGA CATCGTGAAG TCCAAGGTCT TCTATTGGCT GGTGATTCTC 
ATCGTTGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC TCTCTGGCTG 
ACCCGTTTGC AAGACATTGC CAACCGGGTG CTGCTGTCCC TCTTCACCAC TGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CTATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGTAGCGG TATCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGACA 
CCCCTGGGCA TCTCCGTGCT CCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAA 
TATTGGACGT CGCTGAGCAA CCTGGTGGCA TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC GTCATCTTCG CCCTCCTGGG CATGCAGCTC 
TTTGGGGGGA GGTATGACTT TGAAGACACA GAAGTACGGC GCAGCAACTT TGACAACTTT 
CCCCAAGCCC TCATCAGCGT CTTCCAGGTA CTGACAGGGG AAGACTGGAC CTCAATGATG 
TACAATGGGA TCATGGCCTA CGGCGGGCCG TCCTACCCTG GCATGCTTGT GTGCATTTAC 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTGCTCA ATGTCTTCCT GGCCATTGCC 
GTGGACAACC TGGCCGAGGC GGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAGGAG 
AAAAAACGCA GGAAGATGTC CAAGGGTCTC CCAGACAAGT CAGAAGAGGA GAAGTCAACG 
ATGGCCAAGA AGCTGGAGCA GAAACCCAAG GGTGAGGGCA TCCCCACCAC TGCCAAGCTG 
AAAATCGATG AGTTTGAATC TAATGTCAAT GAGGTGAAGG ATCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ATGACGAGGA AGATGAGCCT GAGATCCCGC TGAGCCCCCG ACCACGTCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCC GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAATAA GATCCGTGTC CTGTGTCACC GCATCGTCAA TGCCACCTGG 
TTTACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CTGCACTGGC TGCGGAAGAC 
CCCATCCGGG CTGATTCCAT GAGAAATCAG ATCCTTAAAC ACTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACGACCT ACGGAGCCTT CCTGCACAAG 
GGTTCCTTCT GCCGCAATTA CTTCAACATG CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGTGCCATC TCCGTGGTGA AGATCCTGAG GGTGCTGAGG 
GTGCTCCGAC CACTCAGAGC CATCAACAGA GCCAAGGGGT TGAAGCACGT GGTGCAGTGC 
ATGTTCGTGG CCATCAGCAC CATCGGGAAC ATCGTGCTGG TCACTACCCT CCTACAGTTC 
ATGTTTGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGGTG CACCGACTTG 
TCCAAGATGA CAGAGGAGGA GTGCAGGGGC TACTACTACG TGTACAAGGA CGGGGACCCC 
ATGCAGATAG AGCTGCGTCA CCGCGAGTGG GTACACAGCG ACTTCCACTT CGACAATGTG 
CTCTCAGCCA TGATGTCCCT CTTCACGGTC TCCACCTTCG AGGGATGGCC TCAGCTGCTG 
TACAAGGCCA TAGACTCCAA TGCGGAGGAC GTGGGTCCCA TCTACAACAA CCGTGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGAGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA ATGTGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGC 
TACATTCCCA AAAACCCATA CCAGTACCAG GTGTGGTACA TTGTCACCTC CTCCTACTTT 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTCGGCAT GCAGCACTAC 
AACCAGTCGG AGCAGATGAA CCACATCTCA GACATCCTCA ATGTGGCCTT CACTATCATC 
TTCACCCTGG AGATGATCCT CAAGCTCATG GCCTTCAAGG CCAGGGGCTA CTTTGGAGAC 
CCCTGGAATG TGTTTGACTT CCTGATTGTC ATTGGCAGCA TCATTGATGT CATCCTCAGT 
GAGATCGACG ACCCAGATGA GAGTGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAAGGAG TGCGAACCCT CCTGTGGACG 
TTCATCAAGT CCTTCCAGGC CCTACCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCTG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCTTGGTGGA TGGGACCCAA 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGCTACTGCT CTTCAGGTGT 
GCAACAGGTG AGGCCTGGCA GGAGATCCTA CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCAGAGTCGG ACTATGCCCC AGGGGAGGAG TACACATGTG GCACCAACTT TGCATACTAC 
TACTTCATCA GCTTCTACAT GCTCTGTGCC TTCCTGGTCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ATTTTGACTA CCTCACCCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATCTG GGCAGAGTAT GACCCAGAGG CTAAGGGGAG AATCAAACAC 
CTGGACGTGG TGACCCTGCT GAGAAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACATCGGG TAGCTTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGACGGC 
ACAGTCACCT TCAATGCCAC ACTCTTTGCC CTGGTCCGCA CGGCACTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAGGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTGGGGAAGT TCTACGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAACGC 
CAAGAGGAGT ATTATGGCTA TCGGCCCAAG AAGGACATTG TACAGATCCA GGCAGGGCTG 
CGGACCATTG AGGAAGAGGC AGCCCCCGAG ATCTGTCGCA CGGTCTCAGG AGACCTGGCT 
GCTGAGGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAGGG AATATTCCGG 
AGGACTGGAG GCCTGTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCCGTCATGG CCAATCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGATGGAG 
TCACCTGTCT TCTTGGAGGA CTTCCCACAA GATCCACGCA CCAACCCCCT GGCTCGTGCC 
AATACCAACA ATGCCAACGC CAATGTCGCC TATGGCAACA GCAACCATAG CAACAGCCAT 
GTGTTTTCCA GTGTCCACTA TGAAAGGGAG TTCCCAGAAG AGACAGAGAC GCCTGCTACC 
AGAGGACGAG CCCTTGGCCA ACCCTGCAGG GTCCTGGGAC CCCACAGCAA ACCCTGTGTG 
GAGATGCTGA AGGGACTGCT GACCCAGAGG GCAATGCCCA GAGGCCAGGC ACCTCCTGCC 
CCCTGCCAGT GCCCCAGGGT GGAGTCCTCC ATGCCTGAGG ACAGAAAGAG CTCCACACCA 
GGGTCTCTTC ATGAGGAGAC ACCCCACAGC AGGAGCACCA GGGAGAATAC TTCCAGGTGC 
TCAGCACCAG CTACAGCCCT GCTGATCCAA AAGGCTCTGG TTCGAGGGGG CCTGGGCACC 
TTGGCAGCTG ATGCAAACTT CATCATGGCA ACAGGCCAGG CCCTGGCAGA TGCCTGCCAA 
ATGGAACCAG AGGAAGTGGA GATCATGGCA ACAGAGCTAC TGAAAGGACG AGAGGCCCCA 
GAGGGCATGG CCAGCTCCCT GGGATGCCTG AACCTCGGGT CCTCCCTGGG CAGCCTCGAC 
CAACACCAGG GCTCCCAGGA GACCCTTATT CCTCCAAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Na leak with gating pore properties in hypokalemic periodic paralysis V876E mutant muscle Ca channel.
The Journal of general physiology149(12)1139-1148(2017 Dec)
Fuster C,Perrot J,Berthier C,Jacquemond V,Charnet P,Allard B

Mechanisms Responsible for ?-Pore Currents in Ca
Biophysical journal113(7)1485-1495(2017 Oct)
Monteleone S,Lieb A,Pinggera A,Negro G,Fuchs JE,Hofer F,Striessnig J,Tuluc P,Liedl KR

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CACNA1S cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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