CA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CA3
Entrez Gene ID	761
Full Name	carbonic anhydrase 3
Synonyms	CAIII,Car3
General protein information	Preferred Names carbonic anhydrase 3 Names carbonic anhydrase 3 CA-III HEL-S-167mP carbonate dehydratase III carbonic anhydrase III, muscle specific epididymis secretory sperm binding protein Li 167mP
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q21.2
Summary	Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008].
Disorder MIM:	114750

mRNA and Protein(s)

mRNA	Protein	Name
NM_005181.4	NP_005172.1	carbonic anhydrase 3
NM_005181.3	NP_005172.1	carbonic anhydrase 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Car3	NP_031632.2
Bos taurus (cattle)	CA3	NP_001029609.1
Gallus gallus (chicken)	CA3	NP_001264340.1
Xenopus tropicalis (tropical clawed frog)	ca3	XP_002939197.1
	CA3	XP_001095900.1
Homo sapiens (human)	CA3	NP_005172.1
Pan troglodytes (chimpanzee)	CA3	XP_001164040.2
Canis lupus familiaris (dog)	CA3	NP_001138644.1
Gallus gallus (chicken)	LOC420209	NP_001264339.1
Rattus norvegicus (Norway rat)	Car3	NP_062165.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of CAIII and Hsp70 Is Increased the Mucous Membrane of the Posterior Commissure in Laryngopharyngeal Reflux Disease.
Min HJ, Hong SC, Yang HS, Mun SK, Lee SY
Yonsei medical journal57(2)469-74(2016 Mar)

Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis.
Kawasaki H, Tominaga M, Shigenaga A, Kamo A, Kamata Y, Iizumi K, Kimura U, Ogawa H, Takamori K, Yamakura F
Free radical biology & medicine7375-83(2014 Aug)

Genome-wide association study identifies loci affecting blood copper, selenium and zinc.
Evans DM, Zhu G, Dy V, Heath AC, Madden PA, Kemp JP, McMahon G, St Pourcain B, Timpson NJ, Golding J, Lawlor DA, Steer C, Montgomery GW, Martin NG, Smith GD, Whitfield JB
Human molecular genetics22(19)3998-4006(2013 Oct)

Anti-carbonic anhydrase III autoantibodies in vasculitis syndrome.
Saito R, Watanabe H, Asano T, Suzuki E, Iwadate H, Kobayashi H, Ohira H
International journal of rheumatic diseases16(3)339-46(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis.
Title: Expression of CAIII and Hsp70 Is Increased the Mucous Membrane of the Posterior Commissure in Laryngopharyngeal Reflux Disease.

this study detected the formation and colocalization of 6-nitrotryptophan-containing proteins and CAIII in the skin of atopic dermatitis patients, not only in the lesional part but also in the nonlesional part, through histochemical analyses
Title: Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis.

Report that anti-CAIII antibodies may be useful for diagnosing microscopic polyangiitis.
Title: Anti-carbonic anhydrase III autoantibodies in vasculitis syndrome.

The carbonic anhydrase CA3 isoform displayed an increased abundance during muscle aging, which was independently verified by immunoblotting of differently aged human skeletal muscle samples.
Title: Mass spectrometry-based proteomic analysis of middle-aged vs. aged vastus lateralis reveals increased levels of carbonic anhydrase isoform 3 in senescent human skeletal muscle.

The generation of CA III and IV autoantibodies, antioxidant enzymes, and cytokines might influence each other.
Title: Carbonic anhydrases III and IV autoantibodies in rheumatoid arthritis, systemic lupus erythematosus, diabetes, hypertensive renal disease, and heart failure.

The following CA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13674	NM_005181.4 Latest version!	Homo sapiens carbonic anhydrase 3 (CA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu13674	NM_005181.3	Homo sapiens carbonic anhydrase 3 (CA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CA3 ( NM_005181.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005172.1 Homo sapiens carbonic anhydrase 3 (CA3), mRNA.

Price & Availability↑

CloneID	OHu13674
Clone ID Related Accession (Same CDS sequence)	NM_005181.4 , NM_005181.3
Accession Version	NM_005181.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	carbonic anhydrase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA421884.1, AK313254.1 and AC084734.4. On Nov 23, 2018 this sequence version replaced NM_005181.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.147554.1, SRR5189667.152967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285381.3/ ENSP00000285381.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGG AGTGGGGCTA CGCCAGTCAC AACGGTCCTG ACCACTGGCA TGAACTTTTC 
CCAAATGCCA AGGGGGAAAA CCAGTCGCCC GTTGAGCTGC ATACTAAAGA CATCAGGCAT 
GACCCTTCTC TGCAGCCATG GTCTGTGTCT TATGATGGTG GCTCTGCCAA GACCATCCTG 
AATAATGGGA AGACCTGCCG AGTTGTATTT GATGATACTT ATGATAGGTC AATGCTGAGA 
GGGGGTCCTC TCCCTGGACC CTACCGACTT CGCCAGTTTC ATCTTCACTG GGGCTCTTCG 
GATGATCATG GCTCTGAGCA CACCGTGGAT GGAGTCAAGT ATGCAGCGGA GCTTCATTTG 
GTTCACTGGA ACCCGAAGTA TAACACTTTT AAAGAAGCCC TGAAGCAGCG CGATGGGATC 
GCTGTGATTG GCATTTTTCT GAAGATAGGA CATGAGAATG GCGAGTTCCA GATTTTCCTT 
GATGCATTGG ACAAGATTAA GACAAAGGGC AAGGAGGCGC CCTTCACAAA GTTTGACCCA 
TCCTGCCTGT TCCCGGCATG CCGGGACTAC TGGACCTACC AGGGCTCATT CACCACGCCG 
CCCTGCGAGG AATGCATTGT GTGGCTGCTG CTGAAGGAGC CCATGACCGT GAGCTCTGAC 
CAGATGGCCA AGCTGCGGAG CCTCCTCTCC AGTGCTGAGA ACGAGCCCCC AGTGCCTCTT 
GTGAGCAACT GGCGACCTCC ACAGCCTATC AATAACAGGG TGGTGAGAGC TTCCTTCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005172.1
CDS	52..834
Translation	MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK

Target ORF information:

RefSeq Version	NM_005181.4
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonic anhydrase 3 (CA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005181.4

ORF Insert Sequence:

ATGGCCAAGG AGTGGGGCTA CGCCAGTCAC AACGGTCCTG ACCACTGGCA TGAACTTTTC 
CCAAATGCCA AGGGGGAAAA CCAGTCGCCC GTTGAGCTGC ATACTAAAGA CATCAGGCAT 
GACCCTTCTC TGCAGCCATG GTCTGTGTCT TATGATGGTG GCTCTGCCAA GACCATCCTG 
AATAATGGGA AGACCTGCCG AGTTGTATTT GATGATACTT ATGATAGGTC AATGCTGAGA 
GGGGGTCCTC TCCCTGGACC CTACCGACTT CGCCAGTTTC ATCTTCACTG GGGCTCTTCG 
GATGATCATG GCTCTGAGCA CACCGTGGAT GGAGTCAAGT ATGCAGCGGA GCTTCATTTG 
GTTCACTGGA ACCCGAAGTA TAACACTTTT AAAGAAGCCC TGAAGCAGCG CGATGGGATC 
GCTGTGATTG GCATTTTTCT GAAGATAGGA CATGAGAATG GCGAGTTCCA GATTTTCCTT 
GATGCATTGG ACAAGATTAA GACAAAGGGC AAGGAGGCGC CCTTCACAAA GTTTGACCCA 
TCCTGCCTGT TCCCGGCATG CCGGGACTAC TGGACCTACC AGGGCTCATT CACCACGCCG 
CCCTGCGAGG AATGCATTGT GTGGCTGCTG CTGAAGGAGC CCATGACCGT GAGCTCTGAC 
CAGATGGCCA AGCTGCGGAG CCTCCTCTCC AGTGCTGAGA ACGAGCCCCC AGTGCCTCTT 
GTGAGCAACT GGCGACCTCC ACAGCCTATC AATAACAGGG TGGTGAGAGC TTCCTTCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CA3 ( NM_005181.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005172.1 Homo sapiens carbonic anhydrase 3 (CA3), mRNA.

Price & Availability↑

CloneID	OHu13674
Clone ID Related Accession (Same CDS sequence)	NM_005181.4 , NM_005181.3
Accession Version	NM_005181.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	carbonic anhydrase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA421884.1, AK313254.1 and AC084734.4. On Oct 4, 2008 this sequence version replaced NM_005181.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096880.1, EU794596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCAAGG AGTGGGGCTA CGCCAGTCAC AACGGTCCTG ACCACTGGCA TGAACTTTTC 
CCAAATGCCA AGGGGGAAAA CCAGTCGCCC GTTGAGCTGC ATACTAAAGA CATCAGGCAT 
GACCCTTCTC TGCAGCCATG GTCTGTGTCT TATGATGGTG GCTCTGCCAA GACCATCCTG 
AATAATGGGA AGACCTGCCG AGTTGTATTT GATGATACTT ATGATAGGTC AATGCTGAGA 
GGGGGTCCTC TCCCTGGACC CTACCGACTT CGCCAGTTTC ATCTTCACTG GGGCTCTTCG 
GATGATCATG GCTCTGAGCA CACCGTGGAT GGAGTCAAGT ATGCAGCGGA GCTTCATTTG 
GTTCACTGGA ACCCGAAGTA TAACACTTTT AAAGAAGCCC TGAAGCAGCG CGATGGGATC 
GCTGTGATTG GCATTTTTCT GAAGATAGGA CATGAGAATG GCGAGTTCCA GATTTTCCTT 
GATGCATTGG ACAAGATTAA GACAAAGGGC AAGGAGGCGC CCTTCACAAA GTTTGACCCA 
TCCTGCCTGT TCCCGGCATG CCGGGACTAC TGGACCTACC AGGGCTCATT CACCACGCCG 
CCCTGCGAGG AATGCATTGT GTGGCTGCTG CTGAAGGAGC CCATGACCGT GAGCTCTGAC 
CAGATGGCCA AGCTGCGGAG CCTCCTCTCC AGTGCTGAGA ACGAGCCCCC AGTGCCTCTT 
GTGAGCAACT GGCGACCTCC ACAGCCTATC AATAACAGGG TGGTGAGAGC TTCCTTCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005172.1
CDS	84..866
Translation	MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK

Target ORF information:

RefSeq Version	NM_005181.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonic anhydrase 3 (CA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005181.3

ORF Insert Sequence:

ATGGCCAAGG AGTGGGGCTA CGCCAGTCAC AACGGTCCTG ACCACTGGCA TGAACTTTTC 
CCAAATGCCA AGGGGGAAAA CCAGTCGCCC GTTGAGCTGC ATACTAAAGA CATCAGGCAT 
GACCCTTCTC TGCAGCCATG GTCTGTGTCT TATGATGGTG GCTCTGCCAA GACCATCCTG 
AATAATGGGA AGACCTGCCG AGTTGTATTT GATGATACTT ATGATAGGTC AATGCTGAGA 
GGGGGTCCTC TCCCTGGACC CTACCGACTT CGCCAGTTTC ATCTTCACTG GGGCTCTTCG 
GATGATCATG GCTCTGAGCA CACCGTGGAT GGAGTCAAGT ATGCAGCGGA GCTTCATTTG 
GTTCACTGGA ACCCGAAGTA TAACACTTTT AAAGAAGCCC TGAAGCAGCG CGATGGGATC 
GCTGTGATTG GCATTTTTCT GAAGATAGGA CATGAGAATG GCGAGTTCCA GATTTTCCTT 
GATGCATTGG ACAAGATTAA GACAAAGGGC AAGGAGGCGC CCTTCACAAA GTTTGACCCA 
TCCTGCCTGT TCCCGGCATG CCGGGACTAC TGGACCTACC AGGGCTCATT CACCACGCCG 
CCCTGCGAGG AATGCATTGT GTGGCTGCTG CTGAAGGAGC CCATGACCGT GAGCTCTGAC 
CAGATGGCCA AGCTGCGGAG CCTCCTCTCC AGTGCTGAGA ACGAGCCCCC AGTGCCTCTT 
GTGAGCAACT GGCGACCTCC ACAGCCTATC AATAACAGGG TGGTGAGAGC TTCCTTCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of CAIII and Hsp70 Is Increased the Mucous Membrane of the Posterior Commissure in Laryngopharyngeal Reflux Disease.
Yonsei medical journal57(2)469-74(2016 Mar)
Min HJ,Hong SC,Yang HS,Mun SK,Lee SY

Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis.
Free radical biology & medicine7375-83(2014 Aug)
Kawasaki H,Tominaga M,Shigenaga A,Kamo A,Kamata Y,Iizumi K,Kimura U,Ogawa H,Takamori K,Yamakura F

Genome-wide association study identifies loci affecting blood copper, selenium and zinc.
Human molecular genetics22(19)3998-4006(2013 Oct)
Evans DM,Zhu G,Dy V,Heath AC,Madden PA,Kemp JP,McMahon G,St Pourcain B,Timpson NJ,Golding J,Lawlor DA,Steer C,Montgomery GW,Martin NG,Smith GD,Whitfield JB

Anti-carbonic anhydrase III autoantibodies in vasculitis syndrome.
International journal of rheumatic diseases16(3)339-46(2013 Jun)
Saito R,Watanabe H,Asano T,Suzuki E,Iwadate H,Kobayashi H,Ohira H

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CA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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