VEGFA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VEGFA
Entrez Gene ID	7422
Full Name	vascular endothelial growth factor A
Synonyms	MVCD1,VEGF,VPF
General protein information	Preferred Names vascular endothelial growth factor A Names vascular endothelial growth factor A vascular endothelial growth factor A121 vascular endothelial growth factor A165 vascular permeability factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.1
Summary	This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015].
Disorder MIM:	192240
Disorder Html:	{Microvascular complications of diabetes 1}, 603933 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003376.5	NP_003367.4	vascular endothelial growth factor A isoform b
NM_001033756.2	NP_001028928.1	vascular endothelial growth factor A isoform g
NM_001025370.3	NP_001020541.2	vascular endothelial growth factor A isoform f
NM_001171625.1	NP_001165096.1	vascular endothelial growth factor A isoform k precursor
NM_001204385.2	NP_001191314.1	vascular endothelial growth factor A isoform r
NM_001025367.3	NP_001020538.2	vascular endothelial growth factor A isoform c
NM_001025368.2	NP_001020539.2	vascular endothelial growth factor A isoform d
NM_001025370.2	NP_001020541.2	vascular endothelial growth factor A isoform f
NM_001171628.1	NP_001165099.1	vascular endothelial growth factor A isoform n precursor
NM_001171623.1	NP_001165094.1	vascular endothelial growth factor A isoform i precursor
NM_001025366.3	NP_001020537.2	vascular endothelial growth factor A isoform a
NM_001287044.1	NP_001273973.1	vascular endothelial growth factor A isoform s
NM_001025367.2	NP_001020538.2	vascular endothelial growth factor A isoform c
NM_001025369.2	NP_001020540.2	vascular endothelial growth factor A isoform e
NM_003376.6	NP_003367.4	vascular endothelial growth factor A isoform b
NM_001287044.2	NP_001273973.1	vascular endothelial growth factor A isoform s
NM_001171629.1	NP_001165100.1	vascular endothelial growth factor A isoform o precursor
NM_001171626.1	NP_001165097.1	vascular endothelial growth factor A isoform VEGF-A precursor
NM_001204385.1	NP_001191314.1	vascular endothelial growth factor A isoform r
NM_001025369.3	NP_001020540.2	vascular endothelial growth factor A isoform e
NM_001025368.3	NP_001020539.2	vascular endothelial growth factor A isoform d
NM_001171624.1	NP_001165095.1	vascular endothelial growth factor A isoform j precursor
NM_001204384.1	NP_001191313.1	vascular endothelial growth factor A isoform q precursor
NM_001317010.1	NP_001303939.1	vascular endothelial growth factor A isoform VEGF-Ax precursor
NM_001171622.1	NP_001165093.1	vascular endothelial growth factor A isoform h
NM_001025366.2	NP_001020537.2	vascular endothelial growth factor A isoform a
NM_001033756.3	NP_001028928.1	vascular endothelial growth factor A isoform g
NM_001171622.2	NP_001165093.1	vascular endothelial growth factor A isoform h
NM_001171630.1	NP_001165101.1	vascular endothelial growth factor A isoform p precursor
NM_001171627.1	NP_001165098.1	vascular endothelial growth factor A isoform m precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vegfa	NP_114024.2
Gallus gallus (chicken)	VEGFA	NP_990373.1
Canis lupus familiaris (dog)	VEGFA	NP_001003175.2
Bos taurus (cattle)	VEGFA	NP_776641.1
Mus musculus (house mouse)	Vegfa	NP_001020421.2
Homo sapiens (human)	VEGFA	NP_003367.4
Danio rerio (zebrafish)	vegfab	NP_001038320.2

Gene Ontology
Bibliography

Related articles in PubMed

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

An upstream open reading frame within an IRES controls expression of a specific VEGF-A isoform.
Bastide A, Karaa Z, Bornes S, Hieblot C, Lacazette E, Prats H, Touriol C
Nucleic acids research36(7)2434-45(2008 Apr)

VEGF165b, an inhibitory splice variant of vascular endothelial growth factor, is down-regulated in renal cell carcinoma.
Bates DO, Cui TG, Doughty JM, Winkler M, Sugiono M, Shields JD, Peat D, Gillatt D, Harper SJ
Cancer research62(14)4123-31(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Exploratory study evaluating the association of polymorphisms of angiogenesis genes with hot flashes.

Serum VEGF-A levels are significantly higher in obese patients than in lean controls, decreasing after weight loss with bariatric surgery.
Title: Effects of weight loss after bariatric surgery for morbid obesity on vascular endothelial growth factor-A, adipocytokines, and insulin.

Chromogranin A-, serotonin-, synaptophysin- and vascular endothelial growth factor-positive endocrine cells and the prognosis of colorectal cancer: an immunohistochemical and ultrastructural study.
Title: Chromogranin A-, serotonin-, synaptophysin- and vascular endothelial growth factor-positive endocrine cells and the prognosis of colorectal cancer: an immunohistochemical and ultrastructural study.

VEGF expression in K562 cells treated with artesunate decreased significantly.
Title: [Suppressive effect of artesunate on K562 cell growth and its influence on VEGF expression].

Although limited by sample size, the study did not demonstrated any evidence that VEGF -460 C/T, +405 G/C, and +936 C/T polymorphisms are associated with an increased risk of endometrial carcinomas in Japanese women.
Title: Association study of vascular endothelial growth factor gene polymorphisms in endometrial carcinomas in a Japanese population.

The following VEGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VEGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26665	NM_001171629.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26685	NM_001025366.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$174.50-$244.30 $349.00
OHu26721	NM_001025367.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$164.50-$230.30 $329.00
OHu26819	NM_001171623.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu26825	NM_001171628.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26865	NM_001171627.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26938	NM_001171625.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu26944	NM_001171624.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu27131	NM_001025368.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27146	NM_001171626.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27630	NM_001287044.2 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14341	NM_001204384.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu26728	NM_001171630.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu102720	NM_001317010.1 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu14217	NM_001204385.2 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu26588	NM_001025369.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu26828	NM_001025370.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu26860	NM_003376.6 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu27292	NM_001033756.3 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu27323	NM_001171622.2 Latest version!	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu26828	NM_001025370.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu26721	NM_001025367.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$164.50-$230.30 $329.00
OHu26860	NM_003376.5	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu27630	NM_001287044.1	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14217	NM_001204385.1	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu26588	NM_001025369.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu27131	NM_001025368.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26685	NM_001025366.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$174.50-$244.30 $349.00
OHu27292	NM_001033756.2	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu27323	NM_001171622.1	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VEGFA ( NM_001171629.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165100.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu26665
Clone ID Related Accession (Same CDS sequence)	NM_001171629.1
Accession Version	NM_001171629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform o precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (o, also referred to as VEGF165b) represented in this RefSeq is derived from the AUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AF430806.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47432.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165100.1
CDS	1039..1614
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRSLTRKD

Target ORF information:

RefSeq Version	NM_001171629.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171629.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025366.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020537.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26685
Clone ID Related Accession (Same CDS sequence)	NM_001025366.3 , NM_001025366.2
Accession Version	NM_001025366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001025366.2. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (a) represented in this RefSeq is derived from the 5'-most CUG start codon. This is the longest isoform. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including S85192.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020537.2
CDS	497..1735
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVYVGARCCLMPWSLPGPHPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025366.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025366.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025366.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020537.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

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CloneID	OHu26685
Clone ID Related Accession (Same CDS sequence)	NM_001025366.3 , NM_001025366.2
Accession Version	NM_001025366.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2010 this sequence version replaced NM_001025366.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (a) represented in this RefSeq is derived from the 5'-most CUG start codon. This is the longest isoform. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including S85192.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020537.2
CDS	499..1737
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVYVGARCCLMPWSLPGPHPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025366.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025366.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025367.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020538.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

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CloneID	OHu26721
Clone ID Related Accession (Same CDS sequence)	NM_001025367.3 , NM_001025367.2
Accession Version	NM_001025367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025367.2. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (c) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AJ010438.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55008.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020538.2
CDS	497..1666
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025367.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025367.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025367.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020538.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

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CloneID	OHu26721
Clone ID Related Accession (Same CDS sequence)	NM_001025367.3 , NM_001025367.2
Accession Version	NM_001025367.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025367.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (c) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AJ010438.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55008.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020538.2
CDS	499..1668
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025367.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025367.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171623.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165094.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26819
Clone ID Related Accession (Same CDS sequence)	NM_001171623.1
Accession Version	NM_001171623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform i precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, S85192.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (i, also referred to as VEGF206) represented in this RefSeq is derived from the AUG start codon, and is shorter at the N-terminus, compared to isoform a. CCDS Note: This CCDS, which is supported by the mRNA S85192.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 34457.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S85192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165094.1
CDS	1039..1737
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVYVGARCCLMPWSLPGPHPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001171623.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171623.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTGTACGT TGGTGCCCGC TGCTGTCTAA TGCCCTGGAG CCTCCCTGGC 
CCCCATCCCT GTGGGCCTTG CTCAGAGCGG AGAAAGCATT TGTTTGTACA AGATCCGCAG 
ACGTGTAAAT GTTCCTGCAA AAACACAGAC TCGCGTTGCA AGGCGAGGCA GCTTGAGTTA 
AACGAACGTA CTTGCAGATG TGACAAGCCG AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171628.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165099.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

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CloneID	OHu26825
Clone ID Related Accession (Same CDS sequence)	NM_001171628.1
Accession Version	NM_001171628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform n precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (n, also referred to as VEGF121) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA BC065522.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47434.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, ERR279836.8179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165099.1
CDS	1039..1482
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Target ORF information:

RefSeq Version	NM_001171628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171628.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171627.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165098.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

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CloneID	OHu26865
Clone ID Related Accession (Same CDS sequence)	NM_001171627.1
Accession Version	NM_001171627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform m precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (m, also referred to as VEGF148) represented in this RefSeq is derived from the AUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AF091352.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47435.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165098.1
CDS	1039..1563
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKM

Target ORF information:

RefSeq Version	NM_001171627.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171627.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171625.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165096.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

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CloneID	OHu26938
Clone ID Related Accession (Same CDS sequence)	NM_001171625.1
Accession Version	NM_001171625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform k precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AJ010438.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (k, also referred to as VEGF183) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA AJ010438.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 47433.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165096.1
CDS	1039..1668
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001171625.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171625.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGTCCC 
TGTGGGCCTT GCTCAGAGCG GAGAAAGCAT TTGTTTGTAC AAGATCCGCA GACGTGTAAA 
TGTTCCTGCA AAAACACAGA CTCGCGTTGC AAGGCGAGGC AGCTTGAGTT AAACGAACGT 
ACTTGCAGAT GTGACAAGCC GAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171624.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165095.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

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CloneID	OHu26944
Clone ID Related Accession (Same CDS sequence)	NM_001171624.1
Accession Version	NM_001171624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform j precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (j, also referred to as VEGF189) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA M27281.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 4907.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165095.1
CDS	1039..1686
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001171624.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171624.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025368.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020539.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

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CloneID	OHu27131
Clone ID Related Accession (Same CDS sequence)	NM_001025368.2 , NM_001025368.3
Accession Version	NM_001025368.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025368.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (d) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. This transcript variant is supported by the full-length mRNA BC058855.1. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55012.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020539.2
CDS	499..1614
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025368.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025368.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025368.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020539.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

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CloneID	OHu27131
Clone ID Related Accession (Same CDS sequence)	NM_001025368.2 , NM_001025368.3
Accession Version	NM_001025368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025368.2. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (d) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. This transcript variant is supported by the full-length mRNA BC058855.1. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55012.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138512.439172.1, BC058855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020539.2
CDS	497..1612
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001025368.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025368.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171626.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165097.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

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CloneID	OHu27146
Clone ID Related Accession (Same CDS sequence)	NM_001171626.1
Accession Version	NM_001171626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform VEGF-A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It can initiate translation from four non-AUG (CUG) sites, and also from an in-frame downstream AUG. This RefSeq represents isoform VEGF-A (also referred to as VEGF165 or VEGF-A165), which is derived from the AUG start codon, and is shorter than isoform a. This variant also encodes a C-terminally extended isoform (VEGF-Ax) that results from the use of an alternative in-frame, downstream translation termination codon (UGA). Isoform VEGF-Ax is represented in NM_001317010.1. CCDS Note: This CCDS, which is supported by the mRNA BC058855.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 34458.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165097.1
CDS	1039..1614
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001171626.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171626.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001287044.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273973.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

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CloneID	OHu27630
Clone ID Related Accession (Same CDS sequence)	NM_001287044.1 , NM_001287044.2
Accession Version	NM_001287044.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY066915.1, BX442364.2, BC065522.1 and BC058855.1. On Nov 23, 2013 this sequence version replaced XM_005249363.1. Transcript Variant: This variant (10) has an alternate 5' exon, which results in translation initiation at a downstream AUG start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (s) has a shorter N-terminus and lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.362344.1, SRR1803613.388738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG 
ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT 
GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG 
ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT 
GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG 
CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA 
GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG 
CCGAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273973.1
CDS	136..627
Translation	MAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001287044.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287044.1

ORF Insert Sequence:

ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG 
ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT 
GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG 
ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT 
GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG 
CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA 
GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG 
CCGAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001287044.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273973.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu27630
Clone ID Related Accession (Same CDS sequence)	NM_001287044.1 , NM_001287044.2
Accession Version	NM_001287044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, HY066915.1, BX442364.2, BC065522.1 and BC058855.1. On Jun 2, 2019 this sequence version replaced NM_001287044.1. Transcript Variant: This variant (10) has an alternate 5' exon, which results in translation initiation at a downstream AUG start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (s) has a shorter N-terminus and lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.362344.1, SRR1803613.388738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG 
ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT 
GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG 
ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT 
GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG 
CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA 
GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG 
CCGAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273973.1
CDS	153..644
Translation	MAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001287044.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287044.2

ORF Insert Sequence:

ATGGCAGAAG GAGGAGGGCA GAATCATCAC GAAGTGGTGA AGTTCATGGA TGTCTATCAG 
CGCAGCTACT GCCATCCAAT CGAGACCCTG GTGGACATCT TCCAGGAGTA CCCTGATGAG 
ATCGAGTACA TCTTCAAGCC ATCCTGTGTG CCCCTGATGC GATGCGGGGG CTGCTGCAAT 
GACGAGGGCC TGGAGTGTGT GCCCACTGAG GAGTCCAACA TCACCATGCA GATTATGCGG 
ATCAAACCTC ACCAAGGCCA GCACATAGGA GAGATGAGCT TCCTACAGCA CAACAAATGT 
GAATGCAGAC CAAAGAAAGA TAGAGCAAGA CAAGAAAATC CCTGTGGGCC TTGCTCAGAG 
CGGAGAAAGC ATTTGTTTGT ACAAGATCCG CAGACGTGTA AATGTTCCTG CAAAAACACA 
GACTCGCGTT GCAAGGCGAG GCAGCTTGAG TTAAACGAAC GTACTTGCAG ATGTGACAAG 
CCGAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001204384.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191313.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

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CloneID	OHu14341
Clone ID Related Accession (Same CDS sequence)	NM_001204384.1
Accession Version	NM_001204384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform q precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (q, also referred to as VEGF145) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191313.1
CDS	1039..1554
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVCDKPRR

Target ORF information:

RefSeq Version	NM_001204384.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204384.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171630.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165101.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

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CloneID	OHu26728
Clone ID Related Accession (Same CDS sequence)	NM_001171630.1
Accession Version	NM_001171630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform p precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (p, also referred to as VEGF111) represented in this RefSeq is derived from the AUG start codon, and is shorter than isoform a. CCDS Note: This CCDS, which is supported by the mRNA DQ229900.1, represents a VEGFA isoform derived from an AUG start codon. Alternative translation initiation from multiple upstream non-AUG(CUG) codons is also possible for this gene, as indicated in PMIDs:11352659, 11563986 and 11731620. The longest isoform encoded by this variant, which is derived from the 5'-most CUG start codon, is represented by CCDS 55007.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165101.1
CDS	1039..1452
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRCDKPRR

Target ORF information:

RefSeq Version	NM_001171630.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171630.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001317010.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303939.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

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CloneID	OHu102720
Clone ID Related Accession (Same CDS sequence)	NM_001317010.1
Accession Version	NM_001317010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform VEGF-Ax precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AB021221.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (4) is identical to NM_001171626.1; however, it uses an alternative in-frame, downstream translation termination codon (UGA), which results in a longer, C-terminally extended isoform (VEGF-Ax) compared to isoform VEGF-A (also referred to as VEGF165 or VEGF-A165). Unlike isoform VEGF-A, which is proangiogenic, VEGF-Ax exhibits antiangiogenic activity. As the upstream UGA stop codon has been shown to be recoded as serine (PMID:24949972), its location in the VEGF-Ax isoform (aa 192) is denoted by an 'S'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058855.1, AF022375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 18304943 stop codon readthrough :: PMID: 24949972 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGAGCCG GGCAGGAGGA AGGAGCCTCC 
CTCAGGGTTT CGGGAACCAG ATCTCTCACC AGGAAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303939.1
CDS	1039..1680
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR*AGQEEGASLRVSGTRSLTRKD

Target ORF information:

RefSeq Version	NM_001317010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317010.1

ORF Insert Sequence:

ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGTGAGCCG GGCAGGAGGA AGGAGCCTCC 
CTCAGGGTTT CGGGAACCAG ATCTCTCACC AGGAAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001204385.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191314.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

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CloneID	OHu14217
Clone ID Related Accession (Same CDS sequence)	NM_001204385.2 , NM_001204385.1
Accession Version	NM_001204385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. On Jun 2, 2019 this sequence version replaced NM_001204385.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform r) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191314.1
CDS	497..1552
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVCDKPRR

Target ORF information:

RefSeq Version	NM_001204385.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204385.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001204385.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191314.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu14217
Clone ID Related Accession (Same CDS sequence)	NM_001204385.2 , NM_001204385.1
Accession Version	NM_001204385.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136131.15, M27281.1 and BC065522.1. Transcript Variant: This variant (9) uses an alternate in-frame splice site in the 3' coding region and lacks an alternate in-frame exon compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform r) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191314.1
CDS	499..1554
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVCDKPRR

Target ORF information:

RefSeq Version	NM_001204385.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204385.1

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTATGTGA CAAGCCGAGG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025369.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020540.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu26588
Clone ID Related Accession (Same CDS sequence)	NM_001025369.2 , NM_001025369.3
Accession Version	NM_001025369.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025369.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (e) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF091352.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55011.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020540.2
CDS	499..1563
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKM

Target ORF information:

RefSeq Version	NM_001025369.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025369.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025369.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020540.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu26588
Clone ID Related Accession (Same CDS sequence)	NM_001025369.2 , NM_001025369.3
Accession Version	NM_001025369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF091352.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025369.2. Transcript Variant: This variant (5) lacks an alternate in-frame exon, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (e) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF091352.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55011.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020540.2
CDS	497..1561
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKM

Target ORF information:

RefSeq Version	NM_001025369.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025369.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025370.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020541.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu26828
Clone ID Related Accession (Same CDS sequence)	NM_001025370.3 , NM_001025370.2
Accession Version	NM_001025370.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001025370.2. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (f) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including BC065522.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55014.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, DRR138528.598114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020541.2
CDS	497..1480
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Target ORF information:

RefSeq Version	NM_001025370.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025370.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001025370.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020541.2 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu26828
Clone ID Related Accession (Same CDS sequence)	NM_001025370.3 , NM_001025370.2
Accession Version	NM_001025370.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF214570.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001025370.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (f) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including BC065522.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55014.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065522.1, ERR279836.8179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020541.2
CDS	499..1482
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Target ORF information:

RefSeq Version	NM_001025370.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025370.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_003376.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003367.4 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

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CloneID	OHu26860
Clone ID Related Accession (Same CDS sequence)	NM_003376.5 , NM_003376.6
Accession Version	NM_003376.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_003376.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform b) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003367.4
CDS	499..1686
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_003376.5
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003376.5

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_003376.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_003367.4 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

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CloneID	OHu26860
Clone ID Related Accession (Same CDS sequence)	NM_003376.5 , NM_003376.6
Accession Version	NM_003376.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, M27281.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_003376.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform represented in this RefSeq (isoform b) is derived from the 5'-most CUG start codon, and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.367047.1, SRR1803617.494213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003367.4
CDS	497..1684
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_003376.6
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003376.6

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AAAAAATCAG TTCGAGGAAA GGGAAAGGGG CAAAAACGAA AGCGCAAGAA ATCCCGGTAT 
AAGTCCTGGA GCGTTCCCTG TGGGCCTTGC TCAGAGCGGA GAAAGCATTT GTTTGTACAA 
GATCCGCAGA CGTGTAAATG TTCCTGCAAA AACACAGACT CGCGTTGCAA GGCGAGGCAG 
CTTGAGTTAA ACGAACGTAC TTGCAGATGT GACAAGCCGA GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001033756.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001028928.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

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CloneID	OHu27292
Clone ID Related Accession (Same CDS sequence)	NM_001033756.2 , NM_001033756.3
Accession Version	NM_001033756.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. On Jan 21, 2010 this sequence version replaced NM_001033756.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (g) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF430806.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028928.1
CDS	499..1614
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRSLTRKD

Target ORF information:

RefSeq Version	NM_001033756.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033756.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001033756.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001028928.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu27292
Clone ID Related Accession (Same CDS sequence)	NM_001033756.2 , NM_001033756.3
Accession Version	NM_001033756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, AF430806.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001033756.2. Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (g) represented in this RefSeq is derived from the 5'-most CUG start codon, and has a distinct C-terminus and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including AF430806.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028928.1
CDS	497..1612
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRSLTRKD

Target ORF information:

RefSeq Version	NM_001033756.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033756.3

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA 
AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG 
TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC 
GAACGTACTT GCAGATCTCT CACCAGGAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171622.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165093.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu27323
Clone ID Related Accession (Same CDS sequence)	NM_001171622.1 , NM_001171622.2
Accession Version	NM_001171622.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (h) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including DQ229900.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165093.1
CDS	499..1452
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRCDKPRR

Target ORF information:

RefSeq Version	NM_001171622.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171622.1

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001171622.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165093.1 Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu27323
Clone ID Related Accession (Same CDS sequence)	NM_001171622.1 , NM_001171622.2
Accession Version	NM_001171622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	vascular endothelial growth factor A isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA237741.1, CN256173.1, BU153227.1, DQ229900.1, AF024710.1, BF700556.1 and BM661679.1. On Jun 2, 2019 this sequence version replaced NM_001171622.1. Transcript Variant: This variant (8) lacks three alternate in-frame exons in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (h) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including DQ229900.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ229900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11352659, 11563986, 11731620 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165093.1
CDS	497..1450
Translation	LTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRCDKPRR

Target ORF information:

RefSeq Version	NM_001171622.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171622.2

ORF Insert Sequence:

CTGACGGACA GACAGACAGA CACCGCCCCC AGCCCCAGCT ACCACCTCCT CCCCGGCCGG 
CGGCGGACAG TGGACGCGGC GGCGAGCCGC GGGCAGGGGC CGGAGCCCGC GCCCGGAGGC 
GGGGTGGAGG GGGTCGGGGC TCGCGGCGTC GCACTGAAAC TTTTCGTCCA ACTTCTGGGC 
TGTTCTCGCT TCGGAGGAGC CGTGGTCCGC GCGGGGGAAG CCGAGCCGAG CGGAGCCGCG 
AGAAGTGCTA GCTCGGGCCG GGAGGAGCCG CAGCCGGAGG AGGGGGAGGA GGAAGAAGAG 
AAGGAAGAGG AGAGGGGGCC GCAGTGGCGA CTCGGCGCTC GGAAGCCGGG CTCATGGACG 
GGTGAGGCGG CGGTGTGCGC AGACAGTGCT CCAGCCGCGC GCGCTCCCCA GGCCCTGGCC 
CGGGCCTCGG GCCGGGGAGG AAGAGTAGCT CGCCGAGGCG CCGAGGAGAG CGGGCCGCCC 
CACAGCCCGA GCCGGAGAGG GAGCGCGAGC CGCGCCGGCC CCGGTCGGGC CTCCGAAACC 
ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG 
GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC 
ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG 
ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC 
AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG 
AGCTTCCTAC AGCACAACAA ATGTGAATGC AGATGTGACA AGCCGAGGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

An upstream open reading frame within an IRES controls expression of a specific VEGF-A isoform.
Nucleic acids research36(7)2434-45(2008 Apr)
Bastide A,Karaa Z,Bornes S,Hieblot C,Lacazette E,Prats H,Touriol C

VEGF165b, an inhibitory splice variant of vascular endothelial growth factor, is down-regulated in renal cell carcinoma.
Cancer research62(14)4123-31(2002 Jul)
Bates DO,Cui TG,Doughty JM,Winkler M,Sugiono M,Shields JD,Peat D,Gillatt D,Harper SJ

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VEGFA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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