CLRN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLRN1
Entrez Gene ID	7401
Full Name	clarin 1
Synonyms	RP61,USH3,USH3A
General protein information	Preferred Names clarin 1 Names clarin-1 Usher syndrome type-3 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.1
Summary	This gene encodes a protein that contains a cytosolic N-terminus, multiple helical transmembrane domains, and an endoplasmic reticulum membrane retention signal, TKGH, in the C-terminus. The encoded protein may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIIa. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	606397
Disorder Html:	Usher syndrome, type 3A, 276902 (3); Retinitis pigmentosa 61, 614180 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174878.2	NP_777367.1	clarin-1 isoform a
NM_174878.3	NP_777367.1	clarin-1 isoform a
NM_001195794.1	NP_001182723.1	clarin-1 isoform d
NM_001256819.2	NP_001243748.1	clarin-1 isoform e
NM_001256819.1	NP_001243748.1	clarin-1 isoform e
NM_052995.2	NP_443721.1	clarin-1 isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLRN1	NP_001182723.1
Mus musculus (house mouse)	Clrn1	NP_700434.1
Gallus gallus (chicken)	CLRN1	XP_004943634.1
Canis lupus familiaris (dog)	CLRN1	XP_854563.1
Danio rerio (zebrafish)	clrn1	NP_001002671.1
Xenopus tropicalis (tropical clawed frog)	clrn1	XP_002937147.2
Pan troglodytes (chimpanzee)	CLRN1	XP_003310099.1
Bos taurus (cattle)	CLRN1	NP_001179030.1
Rattus norvegicus (Norway rat)	Clrn1	NP_695211.2
	CLRN1	XP_001107584.1

Gene Ontology
Bibliography

Related articles in PubMed

A deep intronic CLRN1 (USH3A) founder mutation generates an aberrant exon and underlies severe Usher syndrome on the Arabian Peninsula.
Khan AO, Becirovic E, Betz C, Neuhaus C, Altm?ller J, Maria Riedmayr L, Motameny S, N?rnberg G, N?rnberg P, Bolz HJ
Scientific reports7(1)1411(2017 May)

Identification of a novel CLRN1 gene mutation in Usher syndrome type 3: two case reports.
Yoshimura H, Oshikawa C, Nakayama J, Moteki H, Usami S
The Annals of otology, rhinology, and laryngology124 Suppl 194S-9S(2015 May)

Massively parallel DNA sequencing facilitates diagnosis of patients with Usher syndrome type 1.
Yoshimura H, Iwasaki S, Nishio SY, Kumakawa K, Tono T, Kobayashi Y, Sato H, Nagai K, Ishikawa K, Ikezono T, Naito Y, Fukushima K, Oshikawa C, Kimitsuki T, Nakanishi H, Usami S
PloS one9(3)e90688(2014)

Alternative splice variants of the USH3A gene Clarin 1 (CLRN1).
V?stinsalo H, Jalkanen R, Dinculescu A, Isosomppi J, Geller S, Flannery JG, Hauswirth WW, Sankila EM
European journal of human genetics : EJHG19(1)30-5(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first report of Usher syndrome type 3 with a CLRN1 gene mutation in Asian populations.
Title: Identification of a novel CLRN1 gene mutation in Usher syndrome type 3: two case reports.

Possible digenism could not be excluded in two families segregating genomic variations in both MYO7A and USH2A, and two families with CLRN1 and USH2A.
Title: Extended mutation spectrum of Usher syndrome in Finland.

High-resolution measures of retinal structure demonstrate patterns of cone loss associated with CLRN1 mutations.
Title: Cone structure in patients with usher syndrome type III and mutations in the Clarin 1 gene.

This study confirmed using a novel mouse model carrying a Clrn1N48K knock-in mutation to investigate the consequence of the missense mutation N48K in mCLRN1 in vivo.
Title: The mechanosensory structure of the hair cell requires clarin-1, a protein encoded by Usher syndrome III causative gene.

Two novel mutations in the CLRN1 gene, p.R207X and p.I168N, have been found in patients with Usher syndrome type 3.
Title: Two novel disease-causing mutations in the CLRN1 gene in patients with Usher syndrome type 3.

The following CLRN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLRN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18252	NM_001195794.1 Latest version!	Homo sapiens clarin 1 (CLRN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu20117	NM_174878.3 Latest version!	Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19059	NM_052995.2 Latest version!	Homo sapiens clarin 1 (CLRN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu21133	NM_001256819.2 Latest version!	Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu20117	NM_174878.2	Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21133	NM_001256819.1	Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLRN1 ( NM_001195794.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182723.1 Homo sapiens clarin 1 (CLRN1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu18252
Clone ID Related Accession (Same CDS sequence)	NM_001195794.1
Accession Version	NM_001195794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	clarin-1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF482697.1, HM626132.1 and AF495717.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) encodes the longest isoform (d). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM626132.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGTTGCCCT TTGGCTGCCA GCTACCAGGC ACCAGGCTCA AGGCTCCTGT 
GGCTGTCTTG TCATGATATT GTTTGCCTCT GAAGTGAAAA TCCATCACCT CTCAGAAAAA 
ATTGCAAATT ATAAAGAAGG GACTTATGTC TACAAAACGC AAAGTGAAAA ATATACCACC 
TCATTCTGGG TCATTTTCTT TTGCTTTTTT GTTCATTTTC TGAATGGGCT CCTAATACGA 
CTTGCTGGAT TTCAGTTCCC TTTTGCAAAA TCTAAAGACG CAGAAACAAC TAATGTAGCT 
GCAGATCTAA TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182723.1
CDS	292..1029
Translation	MPSQQKKIIFCMAGVFSFACALGVVTALGTPLWIKATVLCKTGALLVNASGQELDKFMGEMQYGLFHGEGVRQCGLGARPFRFSFFPDLLKAIPVSIHVNVILFSAILIVLTMVGTAFFMYNAFGKPFETLHGPLGLYLLSFISVALWLPATRHQAQGSCGCLVMILFASEVKIHHLSEKIANYKEGTYVYKTQSEKYTTSFWVIFFCFFVHFLNGLLIRLAGFQFPFAKSKDAETTNVAADLMY

Target ORF information:

RefSeq Version	NM_001195794.1
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195794.1

ORF Insert Sequence:

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGTTGCCCT TTGGCTGCCA GCTACCAGGC ACCAGGCTCA AGGCTCCTGT 
GGCTGTCTTG TCATGATATT GTTTGCCTCT GAAGTGAAAA TCCATCACCT CTCAGAAAAA 
ATTGCAAATT ATAAAGAAGG GACTTATGTC TACAAAACGC AAAGTGAAAA ATATACCACC 
TCATTCTGGG TCATTTTCTT TTGCTTTTTT GTTCATTTTC TGAATGGGCT CCTAATACGA 
CTTGCTGGAT TTCAGTTCCC TTTTGCAAAA TCTAAAGACG CAGAAACAAC TAATGTAGCT 
GCAGATCTAA TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLRN1 ( NM_174878.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_777367.1 Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20117
Clone ID Related Accession (Same CDS sequence)	NM_174878.2 , NM_174878.3
Accession Version	NM_174878.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	clarin-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF482697.1 and AF495717.1. On or before Oct 10, 2003 this sequence version replaced NM_174879.1, NM_174878.1. Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region, compared to variant 5. The resulting isoform (a) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF495717.1, AF482697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMN03267753 [ECO:0000348] ##Evidence-Data-END##

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGGCTCCTG TGGCTGTCTT GTCATGATAT TGTTTGCCTC TGAAGTGAAA 
ATCCATCACC TCTCAGAAAA AATTGCAAAT TATAAAGAAG GGACTTATGT CTACAAAACG 
CAAAGTGAAA AATATACCAC CTCATTCTGG GTCATTTTCT TTTGCTTTTT TGTTCATTTT 
CTGAATGGGC TCCTAATACG ACTTGCTGGA TTTCAGTTCC CTTTTGCAAA ATCTAAAGAC 
GCAGAAACAA CTAATGTAGC TGCAGATCTA ATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777367.1
CDS	292..990
Translation	MPSQQKKIIFCMAGVFSFACALGVVTALGTPLWIKATVLCKTGALLVNASGQELDKFMGEMQYGLFHGEGVRQCGLGARPFRFSFFPDLLKAIPVSIHVNVILFSAILIVLTMVGTAFFMYNAFGKPFETLHGPLGLYLLSFISGSCGCLVMILFASEVKIHHLSEKIANYKEGTYVYKTQSEKYTTSFWVIFFCFFVHFLNGLLIRLAGFQFPFAKSKDAETTNVAADLMY

Target ORF information:

RefSeq Version	NM_174878.2
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174878.2

ORF Insert Sequence:

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGGCTCCTG TGGCTGTCTT GTCATGATAT TGTTTGCCTC TGAAGTGAAA 
ATCCATCACC TCTCAGAAAA AATTGCAAAT TATAAAGAAG GGACTTATGT CTACAAAACG 
CAAAGTGAAA AATATACCAC CTCATTCTGG GTCATTTTCT TTTGCTTTTT TGTTCATTTT 
CTGAATGGGC TCCTAATACG ACTTGCTGGA TTTCAGTTCC CTTTTGCAAA ATCTAAAGAC 
GCAGAAACAA CTAATGTAGC TGCAGATCTA ATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLRN1 ( NM_174878.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_777367.1 Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20117
Clone ID Related Accession (Same CDS sequence)	NM_174878.2 , NM_174878.3
Accession Version	NM_174878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	clarin-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF482697.1 and AF495717.1. On Jun 2, 2019 this sequence version replaced NM_174878.2. Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region, compared to variant 5. The resulting isoform (a) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF495717.1, AF482697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMN03267753 [ECO:0000348] ##Evidence-Data-END##

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGGCTCCTG TGGCTGTCTT GTCATGATAT TGTTTGCCTC TGAAGTGAAA 
ATCCATCACC TCTCAGAAAA AATTGCAAAT TATAAAGAAG GGACTTATGT CTACAAAACG 
CAAAGTGAAA AATATACCAC CTCATTCTGG GTCATTTTCT TTTGCTTTTT TGTTCATTTT 
CTGAATGGGC TCCTAATACG ACTTGCTGGA TTTCAGTTCC CTTTTGCAAA ATCTAAAGAC 
GCAGAAACAA CTAATGTAGC TGCAGATCTA ATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777367.1
CDS	20..718
Translation	MPSQQKKIIFCMAGVFSFACALGVVTALGTPLWIKATVLCKTGALLVNASGQELDKFMGEMQYGLFHGEGVRQCGLGARPFRFSFFPDLLKAIPVSIHVNVILFSAILIVLTMVGTAFFMYNAFGKPFETLHGPLGLYLLSFISGSCGCLVMILFASEVKIHHLSEKIANYKEGTYVYKTQSEKYTTSFWVIFFCFFVHFLNGLLIRLAGFQFPFAKSKDAETTNVAADLMY

Target ORF information:

RefSeq Version	NM_174878.3
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174878.3

ORF Insert Sequence:

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATTTTTTCC AGATTTGCTC AAAGCAATCC CAGTGAGCAT CCACGTCAAT 
GTCATTCTCT TCTCTGCCAT CCTTATTGTG TTAACCATGG TGGGGACAGC CTTCTTCATG 
TACAATGCTT TTGGAAAACC TTTTGAAACT CTGCATGGTC CCCTAGGGCT GTACCTTTTG 
AGCTTCATTT CAGGCTCCTG TGGCTGTCTT GTCATGATAT TGTTTGCCTC TGAAGTGAAA 
ATCCATCACC TCTCAGAAAA AATTGCAAAT TATAAAGAAG GGACTTATGT CTACAAAACG 
CAAAGTGAAA AATATACCAC CTCATTCTGG GTCATTTTCT TTTGCTTTTT TGTTCATTTT 
CTGAATGGGC TCCTAATACG ACTTGCTGGA TTTCAGTTCC CTTTTGCAAA ATCTAAAGAC 
GCAGAAACAA CTAATGTAGC TGCAGATCTA ATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLRN1 ( NM_052995.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_443721.1 Homo sapiens clarin 1 (CLRN1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu19059
Clone ID Related Accession (Same CDS sequence)	NM_052995.2
Accession Version	NM_052995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	clarin-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF388366.1. This sequence is a reference standard in the RefSeqGene project. On Jan 30, 2003 this sequence version replaced NM_052995.1. Transcript Variant: This variant (4) contains multiple differences in the UTRs and coding region compared to variant 5. The resulting isoform (c) contains shorter and distinct N- and C-termini, compared to isoform d. Sequence Note: This variant (4) is not currently confirmed by EST evidence. The start codon is located within a different exon, compared to variant 1. Two publications (PMID:12145752 and PMID:11524702) support the existence of variant 4. PMID:12145752 suggests that this variant (4) is a minor variant compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF388366.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGGCCC TGCAGCAGCA ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC 
ATCCACGTCA ATGTCATTCT CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA 
GCCTTCTTCA TGTACAATGC TTTTGGAAAA CCTTTTGAAA CTCTGCATGG TCCCCTAGGG 
CTGTACCTTT TGAGCTTCAT TTCAGGCTCC TGTGGCTGTC TTGTCATGAT ATTGTTTGCC 
TCTGAAGTGA AAATCCATCA CCTCTCAGAA AAAATTGCAA ATTATAAAGA AGGGACTTAT 
GTCTACAAAA CGCAAAGTGA AAAATATACC ACCTCATTCT GGCTGACTAA AGGCCACAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443721.1
CDS	393..755
Translation	MQALQQQPVFPDLLKAIPVSIHVNVILFSAILIVLTMVGTAFFMYNAFGKPFETLHGPLGLYLLSFISGSCGCLVMILFASEVKIHHLSEKIANYKEGTYVYKTQSEKYTTSFWLTKGHS

Target ORF information:

RefSeq Version	NM_052995.2
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052995.2

ORF Insert Sequence:

ATGCAGGCCC TGCAGCAGCA ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC 
ATCCACGTCA ATGTCATTCT CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA 
GCCTTCTTCA TGTACAATGC TTTTGGAAAA CCTTTTGAAA CTCTGCATGG TCCCCTAGGG 
CTGTACCTTT TGAGCTTCAT TTCAGGCTCC TGTGGCTGTC TTGTCATGAT ATTGTTTGCC 
TCTGAAGTGA AAATCCATCA CCTCTCAGAA AAAATTGCAA ATTATAAAGA AGGGACTTAT 
GTCTACAAAA CGCAAAGTGA AAAATATACC ACCTCATTCT GGCTGACTAA AGGCCACAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLRN1 ( NM_001256819.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243748.1 Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu21133
Clone ID Related Accession (Same CDS sequence)	NM_001256819.2 , NM_001256819.1
Accession Version	NM_001256819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	clarin-1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020636.14 and BM666773.1. On Jun 2, 2019 this sequence version replaced NM_001256819.1. Transcript Variant: This variant (6) has multiple differences in the coding region, compared to variant 5, one of which results in a translational frameshift. The resulting isoform (e) has a distinct C-terminus and is shorter than isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188 [ECO:0000350] ##Evidence-Data-END##

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATGCTATTT TCTTGACCCC TTCATGGGAC TCCCAACAGG GGTACCCCAT 
TTACTCAGCC TGCCCTGCTC AACCTCTTGC AGGAGGGAGC ACACGAGTGA ACGAGTGCAG 
GAACCAGCTG GCTGCTTTAG TGCTGTGAGG AGTAAACTCC ATGCAGGCCC TGCAGCAGCA 
ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC ATCCACGTCA ATGTCATTCT 
CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA GCCTTCTTCA TGTACAATGC 
TTTTGGAAAA CCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243748.1
CDS	20..577
Translation	MPSQQKKIIFCMAGVFSFACALGVVTALGTPLWIKATVLCKTGALLVNASGQELDKFMGEMQYGLFHGEGVRQCGLGARPFRFSCYFLDPFMGLPTGVPHLLSLPCSTSCRREHTSERVQEPAGCFSAVRSKLHAGPAAATSFSRFAQSNPSEHPRQCHSLLCHPYCVNHGGDSLLHVQCFWKTF

Target ORF information:

RefSeq Version	NM_001256819.2
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256819.2

ORF Insert Sequence:

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATGCTATTT TCTTGACCCC TTCATGGGAC TCCCAACAGG GGTACCCCAT 
TTACTCAGCC TGCCCTGCTC AACCTCTTGC AGGAGGGAGC ACACGAGTGA ACGAGTGCAG 
GAACCAGCTG GCTGCTTTAG TGCTGTGAGG AGTAAACTCC ATGCAGGCCC TGCAGCAGCA 
ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC ATCCACGTCA ATGTCATTCT 
CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA GCCTTCTTCA TGTACAATGC 
TTTTGGAAAA CCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLRN1 ( NM_001256819.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243748.1 Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.

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CloneID	OHu21133
Clone ID Related Accession (Same CDS sequence)	NM_001256819.2 , NM_001256819.1
Accession Version	NM_001256819.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	clarin-1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020636.14 and BM666773.1. Transcript Variant: This variant (6) has multiple differences in the coding region, compared to variant 5, one of which results in a translational frameshift. The resulting isoform (e) has a distinct C-terminus and is shorter than isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188 [ECO:0000350] ##Evidence-Data-END##

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATGCTATTT TCTTGACCCC TTCATGGGAC TCCCAACAGG GGTACCCCAT 
TTACTCAGCC TGCCCTGCTC AACCTCTTGC AGGAGGGAGC ACACGAGTGA ACGAGTGCAG 
GAACCAGCTG GCTGCTTTAG TGCTGTGAGG AGTAAACTCC ATGCAGGCCC TGCAGCAGCA 
ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC ATCCACGTCA ATGTCATTCT 
CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA GCCTTCTTCA TGTACAATGC 
TTTTGGAAAA CCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243748.1
CDS	292..849
Translation	MPSQQKKIIFCMAGVFSFACALGVVTALGTPLWIKATVLCKTGALLVNASGQELDKFMGEMQYGLFHGEGVRQCGLGARPFRFSCYFLDPFMGLPTGVPHLLSLPCSTSCRREHTSERVQEPAGCFSAVRSKLHAGPAAATSFSRFAQSNPSEHPRQCHSLLCHPYCVNHGGDSLLHVQCFWKTF

Target ORF information:

RefSeq Version	NM_001256819.1
Organism	Homo sapiens(human)
Definition	Homo sapiens clarin 1 (CLRN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256819.1

ORF Insert Sequence:

ATGCCAAGCC AACAGAAGAA AATCATTTTT TGCATGGCCG GAGTGTTCAG TTTTGCATGT 
GCCCTCGGAG TTGTGACAGC CTTGGGGACA CCGTTGTGGA TCAAAGCCAC TGTCCTCTGC 
AAAACGGGAG CTCTGCTCGT CAATGCCTCA GGGCAGGAGC TGGACAAGTT TATGGGTGAA 
ATGCAGTACG GGCTTTTCCA CGGAGAGGGT GTGAGGCAGT GTGGGTTGGG AGCAAGGCCC 
TTTCGGTTCT CATGCTATTT TCTTGACCCC TTCATGGGAC TCCCAACAGG GGTACCCCAT 
TTACTCAGCC TGCCCTGCTC AACCTCTTGC AGGAGGGAGC ACACGAGTGA ACGAGTGCAG 
GAACCAGCTG GCTGCTTTAG TGCTGTGAGG AGTAAACTCC ATGCAGGCCC TGCAGCAGCA 
ACCAGTTTTT CCAGATTTGC TCAAAGCAAT CCCAGTGAGC ATCCACGTCA ATGTCATTCT 
CTTCTCTGCC ATCCTTATTG TGTTAACCAT GGTGGGGACA GCCTTCTTCA TGTACAATGC 
TTTTGGAAAA CCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A deep intronic CLRN1 (USH3A) founder mutation generates an aberrant exon and underlies severe Usher syndrome on the Arabian Peninsula.
Scientific reports7(1)1411(2017 May)
Khan AO,Becirovic E,Betz C,Neuhaus C,Altm?ller J,Maria Riedmayr L,Motameny S,N?rnberg G,N?rnberg P,Bolz HJ

Identification of a novel CLRN1 gene mutation in Usher syndrome type 3: two case reports.
The Annals of otology, rhinology, and laryngology124 Suppl 194S-9S(2015 May)
Yoshimura H,Oshikawa C,Nakayama J,Moteki H,Usami S

Massively parallel DNA sequencing facilitates diagnosis of patients with Usher syndrome type 1.
PloS one9(3)e90688(2014)
Yoshimura H,Iwasaki S,Nishio SY,Kumakawa K,Tono T,Kobayashi Y,Sato H,Nagai K,Ishikawa K,Ikezono T,Naito Y,Fukushima K,Oshikawa C,Kimitsuki T,Nakanishi H,Usami S

Alternative splice variants of the USH3A gene Clarin 1 (CLRN1).
European journal of human genetics : EJHG19(1)30-5(2011 Jan)
V?stinsalo H,Jalkanen R,Dinculescu A,Isosomppi J,Geller S,Flannery JG,Hauswirth WW,Sankila EM

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CLRN1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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