USH2A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USH2A
Entrez Gene ID	7399
Full Name	usherin
Synonyms	RP39,US2,USH2,dJ1111A8.1
General protein information	Preferred Names usherin Names usherin Usher syndrome 2A (autosomal recessive, mild) usher syndrome type IIa protein usher syndrome type-2A protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q41
Summary	This gene encodes a protein that contains laminin EGF motifs, a pentaxin domain, and many fibronectin type III motifs. The protein is found in the basement membrane, and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008].
Disorder MIM:	608400
Disorder Html:	Usher syndrome, type 2A, 276901 (3); Retinitis pigmentosa 39, 613809 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007123.5	NP_009054.5	usherin isoform A
NM_206933.3	NP_996816.2	usherin isoform B precursor
NM_206933.2	NP_996816.2	usherin isoform B precursor

Pathways from BioSystems

Homologies

	USH2A	XP_002801987.1
Bos taurus (cattle)	USH2A	NP_001178354.1
Mus musculus (house mouse)	Ush2a	NP_067383.3
Rattus norvegicus (Norway rat)	Ush2a	NP_001231686.1
Gallus gallus (chicken)	USH2A	XP_419417.3
Homo sapiens (human)	USH2A	NP_996816.2
Canis lupus familiaris (dog)	USH2A	XP_545710.2
Pan troglodytes (chimpanzee)	USH2A	XP_514197.2
Danio rerio (zebrafish)	ush2a	XP_697435.5

Gene Ontology
Bibliography

Related articles in PubMed

Usher's Syndrome Type II: A Comparative Study of Genetic Mutations and Vestibular System Evaluation.
Magliulo G, Iannella G, Gagliardi S, Iozzo N, Plateroti R, Mariottini A, Torricelli F
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery157(5)853-860(2017 Nov)

UMD-USHbases: a comprehensive set of databases to record and analyse pathogenic mutations and unclassified variants in seven Usher syndrome causing genes.
Baux D, Faug?re V, Larrieu L, Le Gu?dard-M?reuze S, Hamroun D, B?roud C, Malcolm S, Claustres M, Roux AF
Human mutation29(8)E76-87(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

7 patients clinically classified as having USH2, genetic tests confirmed the USH2 diagnosis in 5 cases. Of these, 4 patients showed mutations in the USH2A gene and 1 patient in the ADGRV1 gene. The mutation of the ADGRV1/GPR98 gene has an extremely rare incidence and is associated with a diagnosis of USH type 2C.
Title: Usher's Syndrome Type II: A Comparative Study of Genetic Mutations and Vestibular System Evaluation.

The current study confirmed a more severe progression of the retinal disease in USH1 patients rather than in USH2 patients.
Title: CLINICAL PRESENTATION AND DISEASE COURSE OF USHER SYNDROME BECAUSE OF MUTATIONS IN MYO7A OR USH2A.

Protein-protein interaction assays and co-expression of complex partners reveal that pathogenic mutations in USH1G severely affect formation of the SANS/ush2a/whirlin complex. Translational read-through drug treatment, targeting the c.728C > A (p.S243X) nonsense mutation, restored SANS scaffold function. We conclude that USH1 and USH2 proteins function together in higher order protein complexes.
Title: Characterization of the ternary Usher syndrome SANS/ush2a/whirlin protein complex.

In summary, our studies provide novel insight into the functional relationship between USH1 and USH2 proteins in the cochlea and the retina as well as the disease mechanisms underlying USH1 and USH2.
Title: The roles of USH1 proteins and PDZ domain-containing USH proteins in USH2 complex integrity in cochlear hair cells.

We identified four novel CNVs in three different genes (one duplication in USH2A gene, two duplications in CEP290 gene, and one duplication in RIMS2 gene) in total four families, at a detection rate of 8% (4/50).
Title: Genome-Wide Detection of Copy Number Variations in Unsolved Inherited Retinal Disease.

The following USH2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USH2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17632	NM_007123.5 Latest version!	Homo sapiens usherin (USH2A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu19218	NM_206933.3 Latest version!	Homo sapiens usherin (USH2A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu19218	NM_206933.2	Homo sapiens usherin (USH2A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USH2A ( NM_007123.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_009054.5 Homo sapiens usherin (USH2A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17632
Clone ID Related Accession (Same CDS sequence)	NM_007123.5
Accession Version	NM_007123.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	usherin isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY481573.1 and AF055580.2. On Jan 9, 2009 this sequence version replaced NM_007123.4. Transcript Variant: This variant (1) uses a different splice site in its 3' coding region when compared to variant 2. The resulting protein (isoform A) has a shorter and distinct C-terminus, compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF055580.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END##

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGTA AGTGTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009054.5
CDS	388..5028
Translation	MNCPVLSLGSGFLFQVIEMLIFAYFASISLTESRGLFPRLENVGAFKKVSIVPTQAVCGLPDRSTFCHSSAAAESIQFCTQRFCIQDCPYRSSHPTYTALFSAGLSSCITPDKNDLHPNAHSNSASFIFGNHKSCFSSPPSPKLMASFTLAVWLKPEQQGVMCVIEKTVDGQIVFKLTISEKETMFYYRTVNGLQPPIKVMTLGRILVKKWIHLSVQVHQTKISFFINGVEKDHTPFNARTLSGSITDFASGTVQIGQSLNGLEQFVGRMQDFRLYQVALTNREILEVFSGDLLRLHAQSHCRCPGSHPRVHPLAQRYCIPNDAGDTADNRVSRLNPEAHPLSFVNDNDVGTSWVSNVFTNITQLNQGVTISVDLENGQYQVFYIIIQFFSPQPTEIRIQRKKENSLDWEDWQYFARNCGAFGMKNNGDLEKPDSVNCLQLSNFTPYSRGNVTFSILTPGPNYRPGYNNFYNTPSLQEFVKATQIRFHFHGQYYTTETAVNLRHRYYAVDEITISGRCQCHGHADNCDTTSQPYRCLCSQESFTEGLHCDRCLPLYNDKPFRQGDQVYAFNCKPCQCNSHSKSCHYNISVDPFPFEHFRGGGGVCDDCEHNTTGRNCELCKDYFFRQVGADPSAIDVCKPCDCDTVGTRNGSILCDQIGGQCNCKRHVSGRQCNQCQNGFYNLQELDPDGCSPCNCNTSGTVDGDITCHQNSGQCKCKANVIGLRCDHCNFGFKFLRSFNDVGCEPCQCNLHGSVNKFCNPHSGQCECKKEAKGLQCDTCRENFYGLDVTNCKACDCDTAGSLPGTVCNAKTGQCICKPNVEGRQCNKCLEGNFYLRQNNSFLCLPCNCDKTGTINGSLLCNKSTGQCPCKLGVTGLRCNQCEPHRYNLTIDNFQHCQMCECDSLGTLPGTICDPISGQCLCVPNRQGRRCNQCQPGFYISPGNATGCLPCSCHTTGAVNHICNSLTGQCVCQDASIAGQRCDQCKDHYFGFDPQTGRCQPCNCHLSGALNETCHLVTGQCFCKQFVTGSKCDACVPSASHLDVNNLLGCSKTPFQQPPPRGQVQSSSAINLSWSPPDSPNAHWLTYSLLRDGFEIYTTEDQYPYSIQYFLDTDLLPYTKYSYYIETTNVHGSTRSVAVTYKTKPGVPEGNLTLSYIIPIGSDSVTLTWTTLSNQSGPIEKYILSCAPLAGGQPCVSYEGHETSATIWNLVPFAKYDFSVQACTSGGCLHSLPITVTTAQAPPQRLSPPKMQKISSTELHVEWSPPAELNGIIIRYELYMRRLRSTKETTSEESRVFQSSGWLSPHSFVESANENALKPPQTMTTITGLEPYTKYEFRVLAVNMAGSVSSAWVSERTGESAPVFMIPPSVFPLSSYSLNISWEKPADNVTRGKVVGYDINMLSEQSPQQSIPMAFSQLLHTAKSQELSYTVEGLKPYRIYEFTITLCNSVGCVTSASGAGQTLAAAPAQLRPPLVKGINSTTIHLKWFPPEELNGPSPIYQLERRESSLPALMTTMMKGIRFIGNGYCKFPSSTHPVNTDFTGKCV

Target ORF information:

RefSeq Version	NM_007123.5
Organism	Homo sapiens(human)
Definition	Homo sapiens usherin (USH2A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007123.5

ORF Insert Sequence:

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGTA AGTGTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USH2A ( NM_206933.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_996816.2 Homo sapiens usherin (USH2A), transcript variant 2, mRNA.

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CloneID	OHu19218
Clone ID Related Accession (Same CDS sequence)	NM_206933.3 , NM_206933.2
Accession Version	NM_206933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	usherin isoform B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445650.9, AY481573.1, AF055580.2, AA883599.1, AADB02001158.1 and AC092799.2. On Nov 23, 2018 this sequence version replaced NM_206933.2. Transcript Variant: This variant (2) is the longer transcript and it encodes the longer isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY481573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGCA TTAAGGCCAG CTTTCGAACA AAAGTGCCTG AAGGTTTGAT TGTCTTTGCA 
GCATCACCTG GCAATCAGGA AGAGTATTTT GCACTTCAGT TGAAGAAGGG ACGTCTTTAT 
TTTCTTTTTG ATCCTCAGGG GTCACCAGTG GAAGTAACTA CAACTAATGA TCATGGCAAA 
CAATATAGTG ATGGAAAATG GCATGAAATA ATTGCTATTA GGCATCAGGC TTTTGGCCAA 
ATCACTCTGG ATGGGATATA TACAGGTTCC TCTGCCATCC TGAATGGTAG TACTGTTATT 
GGAGATAACA CAGGAGTCTT TCTGGGAGGG CTCCCGCGAA GTTATACCAT CCTCAGGAAG 
GATCCTGAGA TAATCCAAAA AGGTTTTGTG GGCTGTCTCA AGGATGTACA TTTTATGAAG 
AATTACAATC CGTCAGCTAT TTGGGAACCT CTGGATTGGC AGAGTTCTGA AGAACAAATC 
AACGTGTATA ACAGCTGGGA GGGATGTCCC GCTTCATTAA ATGAGGGAGC TCAGTTCCTA 
GGAGCAGGGT TCCTGGAACT TCATCCATAT ATGTTTCATG GTGGAATGAA CTTTGAGATT 
TCCTTTAAGT TCAGAACTGA CCAATTAAAT GGATTGCTTC TTTTCGTTTA TAACAAAGAT 
GGACCTGATT TTCTTGCTAT GGAGCTGAAA AGTGGAATAT TGACCTTCCG GTTAAATACC 
AGTCTTGCCT TTACACAAGT GGATCTATTG CTGGGGCTAT CCTATTGTAA TGGAAAGTGG 
AATAAAGTCA TTATTAAAAA GGAAGGCTCT TTCATATCAG CAAGTGTGAA TGGACTGATG 
AAGCATGCAT CGGAGTCCGG AGACCAGCCA CTGGTGGTGA ATTCACCAGT TTATGTGGGA 
GGAATCCCAC AGGAACTGCT GAACTCTTAT CAACATTTGT GTTTGGAACA AGGTTTCGGT 
GGTTGCATGA AGGATGTTAA ATTTACACGG GGTGCTGTCG TTAACTTGGC ATCTGTGTCC 
AGCGGTGCTG TCAGAGTCAA TCTGGATGGA TGCCTATCAA CTGACAGTGC TGTTAACTGC 
AGGGGAAATG ACTCCATCCT GGTTTACCAG GGAAAAGAGC AGAGTGTTTA CGAGGGTGGT 
CTCCAGCCTT TTACAGAATA CCTGTATCGA GTGATAGCCT CGCATGAAGG AGGTTCAGTA 
TATAGTGATT GGAGTCGAGG ACGTACAACA GGAGCAGCTC CACAAAGTGT GCCAACTCCC 
TCAAGAGTCC GCAGCTTAAA TGGATACAGC ATTGAGGTGA CCTGGGATGA ACCTGTTGTC 
AGAGGTGTAA TTGAGAAGTA CATTCTGAAA GCCTATAGTG AGGACAGCAC CCGTCCACCC 
CGCATGCCCT CTGCCAGTGC TGAATTTGTC AATACAAGCA ACCTCACAGG CATATTGACA 
GGCTTGCTAC CCTTCAAAAA CTATGCAGTA ACCCTAACTG CTTGCACTTT GGCTGGCTGT 
ACTGAGAGCT CACATGCATT GAACATCTCT ACTCCACAAG AAGCCCCACA AGAGGTTCAG 
CCACCAGTAG CCAAATCCCT TCCCAGTTCT TTGCTGCTCT CCTGGAACCC ACCCAAAAAG 
GCAAATGGTA TTATAACTCA GTACTGTTTA TACATGGATG GGAGGCTGAT CTATTCAGGC 
AGTGAGGAGA ACTACACAGT CACAGATTTA GCAGTATTTA CACCCCACCA GTTTCTACTA 
AGTGCATGCA CACATGTGGG CTGTACAAAC AGTTCCTGGG TCCTACTGTA CACAGCACAG 
CTGCCACCAG AACACGTGGA TTCCCCAGTT CTGACTGTCC TGGATTCTAG AACTATACAC 
ATACAGTGGA AACAACCAAG AAAAATAAGT GGGATTCTGG AACGCTATGT ATTATATATG 
TCAAACCATA CACATGATTT TACAATTTGG AGTGTCATCT ATAACAGTAC AGAACTTTTC 
CAGGATCATA TGCTACAATA CGTTTTACCT GGTAATAAAT ATCTCATCAA GCTGGGAGCT 
TGCACAGGTG GTGGGTGCAC AGTGAGTGAG GCCAGTGAGG CCCTAACTGA CGAGGACATA 
CCCGAAGGCG TGCCAGCCCC CAAAGCCCAC TCATATTCAC CTGACTCCTT TAATGTCTCC 
TGGACTGAGC CTGAATATCC GAATGGTGTT ATCACGAGTT ATGGATTATA TCTAGATGGT 
ATATTAATCC ACAATTCCTC AGAACTCAGC TATCGTGCTT ACGGATTTGC TCCTTGGAGT 
TTACATTCCT TCAGAGTCCA AGCATGCACG GCCAAAGGTT GTGCTCTGGG CCCACTGGTG 
GAAAATCGAA CTCTAGAAGC TCCTCCTGAA GGAACAGTAA ATGTGTTTGT CAAAACACAG 
GGATCCCGGA AAGCCCACGT GAGGTGGGAA GCACCTTTTC GCCCTAATGG ACTCTTAACA 
CACTCAGTCC TTTTCACTGG GATATTCTAT GTAGACCCAG TAGGTAATAA CTACACCCTT 
CTGAATGTCA CAAAAGTCAT GTACAGCGGA GAAGAGACAA ACCTTTGGGT GCTCATCGAT 
GGGCTGGTTC CTTTTACCAA CTATACTGTA CAAGTGAATA TTTCAAATAG CCAAGGCAGC 
TTGATAACTG ATCCTATAAC AATTGCAATG CCTCCAGGAG CTCCAGATGG CGTGCTGCCT 
CCCAGGCTTT CATCTGCCAC TCCAACCAGT CTTCAGGTTG TCTGGTCTAC ACCAGCTCGT 
AATAACGCTC CTGGCTCTCC CAGATACCAA CTCCAGATGA GGTCTGGCGA CTCCACCCAT 
GGATTTCTAG AGTTATTTTC CAATCCTTCT GCATCGTTAA GCTATGAAGT GAGTGATCTC 
CAACCGTACA CAGAGTATAT GTTTCGGTTG GTTGCCTCCA ATGGATTTGG CAGTGCACAT 
AGTTCTTGGA TTCCATTCAT GACCGCAGAG GACAAACCTG GACCTGTAGT TCCTCCGATT 
CTTCTGGATG TGAAGTCAAG AATGATGTTG GTCACCTGGC AGCATCCTAG AAAATCCAAT 
GGGGTTATTA CCCATTATAA CATTTATCTA CATGGCCGTC TATACTTGAG AACTCCTGGA 
AATGTCACTA ATTGCACAGT GATGCATTTA CACCCATACA CTGCCTATAA GTTTCAGGTA 
GAAGCCTGCA CTTCAAAAGG ATGTTCCCTT TCACCAGAGT CCCAGACTGT ATGGACACTC 
CCAGGGGCAC CGGAAGGGAT CCCAAGTCCA GAGCTGTTCT CTGATACTCC AACATCTGTG 
ATTATATCTT GGCAACCCCC TACCCACCCC AATGGCTTGG TGGAGAATTT CACAATTGAG 
AGAAGAGTCA AAGGAAAGGA AGAAGTTACT ACCCTGGTGA CTCTCCCGAG GAGTCATTCC 
ATGAGGTTTA TTGACAAGAC TTCTGCTCTT AGCCCATGGA CAAAATATGA ATATCGGGTA 
CTGATGAGCA CTCTTCATGG AGGCACAAAC AGCAGTGCTT GGGTAGAAGT TACCACAAGA 
CCCTCACGAC CTGCTGGGGT GCAGCCACCT GTGGTGACAG TGCTGGAACC CGATGCAGTC 
CAGGTCACTT GGAAACCCCC ACTCATCCAG AACGGAGACA TACTTAGCTA TGAGATTCAC 
ATGCCTGACC CTCACATCAC TTTAACCAAT GTGACTTCCG CAGTGTTAAG TCAAAAAGTT 
ACTCATCTGA TTCCTTTCAC TAATTATTCT GTCACCATTG TTGCTTGCTC AGGGGGTAAT 
GGGTACCTTG GAGGGTGCAC AGAGAGTTTA CCTACCTATG TTACCACTCA CCCCACCGTA 
CCTCAGAATG TTGGCCCATT GTCTGTGATT CCACTAAGTG AATCATATGT TGTGATTTCT 
TGGCAACCAC CATCCAAGCC AAATGGACCT AATTTGAGAT ATGAGCTTCT GAGACGTAAA 
ATCCAGCAGC CACTTGCATC AAATCCCCCA GAAGATTTAA ATCGGTGGCA CAATATTTAT 
TCAGGAACTC AGTGGCTTTA TGAAGATAAG GGTCTTAGCA GGTTTACAAC CTATGAATAT 
ATGCTCTTCG TACACAACAG TGTGGGTTTT ACACCGAGCC GAGAAGTGAC TGTGACAACG 
TTAGCTGGTC TTCCAGAGAG AGGAGCCAAT CTCACTGCGA GTGTCCTTAA CCACACAGCC 
ATCGACGTGA GGTGGGCTAA ACCAACTGTT CAAGACCTAC AAGGTGAAGT TGAATATTAC 
ACACTTTTTT GGAGTTCTGC TACCTCAAAC GACTCTCTAA AAATCTTGCC AGATGTAAAC 
TCTCATGTCA TTGGCCACCT AAAGCCAAAC ACAGAGTATT GGATCTTTAT CTCTGTCTTC 
AATGGAGTCC ACAGCATCAA CAGTGCAGGA CTTCATGCAA CCACTTGCGA TGGGGAGCCT 
CAGGGCATGC TTCCTCCAGA GGTTGTCATC ATCAACAGTA CAGCTGTACG TGTCATCTGG 
ACATCTCCTT CAAACCCAAA TGGTGTTGTC ACTGAGTATT CTATCTATGT AAATAATAAG 
CTCTACAAGA CTGGAATGAA TGTGCCTGGG TCGTTTATTC TGAGAGACCT GTCTCCCTTC 
ACTATCTATG ACATTCAGGT TGAAGTCTGC ACAATATATG CCTGCGTGAA AAGCAATGGA 
ACCCAAATTA CCACTGTGGA AGACACTCCA AGTGATATAC CAACACCCAC AATTCGTGGC 
ATCACTTCAA GATCTCTTCA AATTGATTGG GTGTCTCCAC GGAAGCCAAA TGGCATCATT 
CTTGGATATG ATCTCCTATG GAAAACATGG TATCCATGCG CTAAAACTCA AAAGTTAGTG 
CAGGATCAGA GTGATGAGCT CTGCAAGGCA GTGAGGTGTC AAAAACCTGA ATCTATCTGT 
GGACACATTT GCTATTCTTC TGAAGCTAAG GTTTGTTGTA ACGGAGTGCT CTATAACCCC 
AAGCCTGGAC ATCGCTGTTG TGAAGAAAAG TATATCCCGT TTGTTCTGAA TTCTACTGGA 
GTTTGTTGTG GTGGCCGAAT ACAGGAGGCA CAACCAAATC ATCAGTGCTG CTCTGGGTAT 
TACGCTAGAA TTCTACCAGG TGAAGTATGC TGTCCAGATG AACAGCACAA TCGGGTTTCT 
GTTGGCATTG GTGATTCCTG CTGTGGCAGA ATGCCGTACT CCACCTCAGG AAACCAGATT 
TGCTGTGCTG GGAGGCTTCA TGATGGCCAT GGCCAGAAGT GCTGTGGCAG ACAGATTGTG 
AGCAACGATT TAGAGTGTTG TGGTGGAGAA GAAGGAGTGG TGTACAATCG CCTTCCAGGT 
ATGTTCTGTT GTGGGCAGGA TTATGTGAAT ATGTCAGATA CCATATGCTG CTCAGCTTCC 
AGTGGAGAGT CTAAAGCACA TATTAAAAAG AATGACCCGG TGCCAGTAAA ATGCTGTGAG 
ACTGAACTTA TTCCAAAGAG CCAGAAATGC TGTAATGGAG TTGGATATAA TCCTTTGAAA 
TATGTTTGCT CTGACAAGAT TTCAACTGGA ATGATGATGA AGGAAACCAA AGAGTGCAGG 
ATCCTCTGCC CAGCATCTAT GGAAGCCACA GAACATTGTG GCAGGTGTGA CTTCAACTTT 
ACCAGCCACA TTTGCACTGT GATAAGAGGG TCTCACAATT CCACAGGGAA GGCATCAATT 
GAAGAAATGT GTTCATCTGC CGAAGAAACC ATTCATACAG GGAGTGTAAA CACGTACTCT 
TACACAGATG TGAACCTCAA GCCCTACATG ACATATGAGT ACAGGATTTC TGCCTGGAAC 
AGCTATGGGC GAGGACTCAG CAAAGCTGTG AGAGCCAGAA CAAAAGAAGA TGTGCCTCAA 
GGAGTGAGTC CCCCTACGTG GACCAAAATA GACAATCTTG AAGATACAAT TGTCTTAAAC 
TGGAGAAAAC CTATACAATC AAATGGTCCT ATTATTTACT ACATCCTTCT TCGAAATGGA 
ATTGAACGTT TTCGGGGAAC ATCACTGAGC TTCTCTGATA AAGAGGGAAT TCAACCATTT 
CAGGAATATT CATATCAGCT GAAAGCTTGC ACGGTTGCTG GCTGTGCCAC CAGTAGCAAG 
GTAGTTGCAG CTACTACCCA AGGAGTTCCG GAGAGCATCC TGCCACCAAG CATCACAGCC 
CTAAGTGCAG TGGCTCTGCA TCTGAGCTGG AGTGTCCCTG AGAAATCAAA CGGCGTCATT 
AAAGAGTACC AGATCAGGCA GGTTGGGAAA GGTCTCATCC ACACTGACAC CACTGACAGG 
AGACAGCATA CGGTCACAGG TCTCCAGCCA TACACCAACT ACAGCTTCAC TCTTACAGCT 
TGTACATCTG CTGGGTGCAC TTCAAGCGAG CCTTTTCTAG GTCAGACACT GCAGGCAGCT 
CCTGAAGGAG TTTGGGTGAC ACCTCGACAC ATTATCATCA ATTCTACAAC AGTGGAATTA 
TATTGGAGTC TGCCAGAAAA GCCCAATGGC CTCGTTTCTC AATATCAATT GAGTCGTAAT 
GGAAACTTGC TTTTCCTGGG TGGCAGTGAG GAGCAGAATT TCACTGATAA AAACCTGGAG 
CCCAATAGCA GATACACTTA CAAGTTAGAA GTCAAAACTG GAGGTGGCAG CAGTGCTAGT 
GATGATTACA TTGTTCAAAC ACCTATGTCA ACACCAGAAG AAATCTATCC TCCATATAAT 
ATCACAGTAA TTGGGCCTTA TTCTATATTT GTAGCTTGGA TACCACCAGG GATCCTCATC 
CCCGAAATTC CTGTGGAGTA CAATGTCTTA CTCAATGATG GAAGTGTAAC ACCTCTGGCC 
TTCTCCGTTG GTCATCATCA ATCCACCCTT CTGGAAAATT TGACTCCATT CACACAGTAT 
GAGATAAGGA TACAAGCATG TCAAAATGGA AGTTGTGGAG TTAGCAGTAG GATGTTTGTC 
AAAACACCTG AAGCAGCCCC AATGGATCTT AATTCTCCTG TTCTTAAGGC ACTGGGGTCA 
GCTTGCATAG AGATTAAGTG GATGCCACCT GAAAAACCAA ATGGAATCAT CATCAACTAC 
TTTATTTACA GACGCCCTGC TGGCATTGAA GAGGAGTCTG TTTTATTTGT CTGGTCAGAA 
GGAGCCCTTG AATTTATGGA TGAAGGAGAC ACCCTGAGGC CTTTCACACT CTACGAATAT 
CGGGTCAGAG CCTGTAACTC CAAGGGTTCA GTGGAGAGTC TGTGGTCATT AACACAAACT 
CTGGAAGCTC CACCTCAAGA TTTTCCAGCT CCTTGGGCTC AAGCCACGAG TGCTCATTCA 
GTTCTGTTGA ATTGGACAAA GCCAGAATCT CCCAATGGCA TTATCTCCCA TTACCGTGTG 
GTCTACCAGG AGAGACCCGA CGATCCTACA TTTAACAGCC CTACCGTGCA TGCTTTCACA 
GTGAAGGGAA CAAGCCATCA AGCCCACCTG TACGGGTTAG AACCATTCAC AACATATCGC 
ATTGGTGTTG TGGCTGCAAA CCATGCAGGA GAAATTTTAA GCCCTTGGAC TCTGATTCAA 
ACCTTAGAAT CTTCCCCAAG TGGACTGAGA AACTTTATAG TAGAACAGAA AGAGAATGGC 
CGGGCATTGC TACTACAGTG GTCAGAACCT ATGAGAACCA ATGGTGTGAT TAAGACATAC 
AACATCTTCA GTGACGGGTT CCTGGAGTAC TCTGGTTTGA ATCGTCAGTT TCTCTTCCGC 
CGCCTGGATC CTTTCACTCT CTACACACTG ACCCTGGAGG CCTGCACCAG AGCAGGTTGT 
GCACACTCGG CGCCTCAGCC TCTGTGGACA GATGAAGCCC CTCCAGACTC TCAGCTGGCT 
CCTACTGTCC ACTCTGTGAA GTCCACCAGT GTTGAGCTGA GCTGGTCTGA GCCTGTTAAC 
CCAAATGGAA AAATAATTCG CTATGAAGTG ATTCGCAGAT GCTTCGAGGG AAAAGCTTGG 
GGAAATCAGA CGATCCAGGC CGACGAGAAA ATTGTTTTCA CAGAATATAA CACTGAAAGG 
AATACATTTA TGTATAATGA CACAGGTTTG CAACCATGGA CGCAGTGTGA ATATAAAATC 
TACACTTGGA ATTCAGCTGG GCATACCTGT AGCTCTTGGA ATGTGGTGAG GACATTGCAA 
GCACCTCCAG AAGGTCTCTC TCCACCTGTG ATATCCTATG TTTCTATGAA TCCCCAAAAA 
CTGCTGATTT CCTGGATCCC ACCAGAACAG TCTAATGGTA TTATCCAGTC CTATAGGCTT 
CAAAGGAATG AAATGCTCTA TCCTTTTAGC TTTGATCCTG TGACTTTCAA TTACACTGAT 
GAAGAGCTTC TTCCTTTTTC CACCTATAGC TATGCACTCC AAGCCTGCAC GAGTGGAGGA 
TGCTCCACCA GCAAACCCAC CAGCATCACA ACTCTGGAGG CTGCTCCATC AGAAGTCAGC 
CCTCCAGATC TTTGGGCCGT CAGTGCCACT CAAATGAATG TATGTTGGTC ACCGCCCACA 
GTGCAAAATG GAAAGATTAC TAAATATTTA GTTAGATATG ATAATAAAGA GTCCCTTGCT 
GGCCAGGGCC TGTGCCTGCT GGTTTCCCAC CTGCAGCCTT ACTCTCAGTA TAACTTCTCC 
CTTGTAGCCT GCACGAATGG AGGTTGCACA GCTAGTGTGT CAAAATCTGC CTGGACAATG 
GAGGCCCTGC CAGAGAACAT GGACTCTCCA ACATTGCAAG TCACAGGCTC AGAATCAATA 
GAAATCACCT GGAAACCTCC AAGAAACCCA AATGGCCAGA TCAGAAGTTA TGAACTTAGG 
AGGGATGGAA CCATTGTATA TACAGGCTTG GAAACACGCT ATCGTGATTT TACTCTCACC 
CCAGGTGTGG AGTATAGCTA CACAGTAACT GCCAGCAACA GCCAAGGGGG TATTTTGAGT 
CCTCTTGTCA AAGATCGAAC CAGCCCCTCA GCACCCTCAG GGATGGAACC TCCAAAATTG 
CAGGCCAGGG GTCCTCAGGA GATCTTAGTG AACTGGGACC CTCCAGTGAG AACAAATGGT 
GATATCATCA ATTATACCCT CTTCATCCGT GAACTATTTG AAAGAGAAAC TAAAATCATA 
CACATAAACA CAACTCATAA TTCTTTTGGT ATGCAGTCAT ATATAGTAAA CCAGCTGAAG 
CCATTTCACA GGTATGAAAT ACGAATTCAA GCGTGCACCA CCCTGGGATG TGCATCAAGT 
GACTGGACAT TCATACAGAC CCCTGAGATT GCACCTTTGA TGCAACCCCC TCCACATCTG 
GAGGTACAAA TGGCTCCAGG AGGATTCCAG CCAACTGTTT CTCTTTTGTG GACAGGACCG 
CTGCAGCCAA ATGGAAAAGT TTTGTATTAC GAATTATACA GAAGACAAAT AGCAACTCAG 
CCTAGAAAAT CCAATCCAGT CCTAATCTAT AACGGAAGCT CAACATCTTT TATAGATTCC 
GAACTATTGC CTTTCACAGA GTATGAGTAT CAGGTCTGGG CAGTGAATTC TGCAGGAAAA 
GCCCCCAGTA GCTGGACATG GTGCAGAACC GGGCCAGCCC CACCAGAAGG TCTCAGAGCC 
CCCACGTTCC ATGTGATCTC TTCTACCCAA GCAGTGGTCA ACATCAGTGC CCCTGGGAAG 
CCCAACGGGA TCGTCAGTCT CTACAGGCTG TTCTCCAGCA GCGCCCATGG GGCTGAGACA 
GTGCTATCCG AAGGCATGGC CACCCAGCAG ACTCTCCATG GCCTTCAAGC CTTCACTAAC 
TACTCTATTG GAGTAGAGGC CTGCACCTGC TTCAACTGTT GCAGCAAAGG ACCGACAGCT 
GAACTGAGAA CCCATCCTGC CCCACCCTCA GGACTGTCCT CTCCACAAAT CGGGACGCTG 
GCCTCAAGGA CGGCCTCCTT CCGGTGGAGT CCCCCCATGT TCCCCAATGG TGTCATTCAC 
AGCTATGAAC TCCAATTCCA CGTGGCTTGC CCTCCTGACT CAGCCCTCCC CTGTACTCCC 
AGCCAAATAG AAACAAAGTA CACGGGGCTG GGGCAGAAAG CCAGCCTTGG GGGTCTCCAG 
CCCTACACCA CATACAAGCT GAGAGTGGTG GCACACAACG AGGTGGGCAG TACGGCTTCC 
GAGTGGATCA GTTTCACCAC CCAAAAAGAA TTGCCTCAGT ACCGAGCCCC ATTTTCGGTG 
GACAGCAATT TGTCTGTGGT GTGTGTGAAC TGGAGTGACA CCTTCCTCCT GAACGGCCAA 
CTGAAGGAGT ACGTGTTAAC CGACGGAGGG CGACGCGTGT ACAGCGGCTT GGACACCACC 
CTCTACATAC CGAGAACGGC GGACAAAACC TTCTTTTTCC AGGTCATCTG CACGACTGAC 
GAAGGAAGTG TTAAGACGCC GTTGATCCAA TATGATACCT CTACTGGACT TGGCTTGGTC 
CTAACAACTC CTGGGAAAAA GAAGGGATCG CGGAGCAAAA GCACAGAGTT CTACAGCGAG 
CTGTGGTTCA TAGTGTTAAT GGCGATGCTG GGCTTGATCT TGTTGGCCAT TTTTCTGTCC 
CTGATACTAC AAAGAAAAAT CCACAAAGAG CCATATATCA GAGAAAGACC TCCCTTGGTA 
CCTCTTCAGA AGAGGATGTC TCCATTGAAT GTTTACCCAC CGGGGGAAAA CCATATGGGG 
TTAGCCGATA CCAAAATTCC CCGGTCTGGG ACACCTGTGA GTATCCGCAG CAACCGGAGT 
GCATGTGTCC TGCGCATCCC GAGTCAAAAC CAAACCAGCC TAACCTACTC CCAGGGTTCT 
CTTCACCGCA GCGTCAGCCA GCTCATGGAC ATTCAAGACA AGAAAGTCTT GATGGACAAC 
TCACTGTGGG AAGCCATCAT GGGCCACAAC AGTGGACTGT ATGTGGATGA AGAGGACCTG 
ATGAACGCCA TCAAGGATTT CAGCTCAGTG ACTAAGGAAC GCACCACATT CACAGACACC 
CACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996816.2
CDS	440..16048
Translation	MNCPVLSLGSGFLFQVIEMLIFAYFASISLTESRGLFPRLENVGAFKKVSIVPTQAVCGLPDRSTFCHSSAAAESIQFCTQRFCIQDCPYRSSHPTYTALFSAGLSSCITPDKNDLHPNAHSNSASFIFGNHKSCFSSPPSPKLMASFTLAVWLKPEQQGVMCVIEKTVDGQIVFKLTISEKETMFYYRTVNGLQPPIKVMTLGRILVKKWIHLSVQVHQTKISFFINGVEKDHTPFNARTLSGSITDFASGTVQIGQSLNGLEQFVGRMQDFRLYQVALTNREILEVFSGDLLRLHAQSHCRCPGSHPRVHPLAQRYCIPNDAGDTADNRVSRLNPEAHPLSFVNDNDVGTSWVSNVFTNITQLNQGVTISVDLENGQYQVFYIIIQFFSPQPTEIRIQRKKENSLDWEDWQYFARNCGAFGMKNNGDLEKPDSVNCLQLSNFTPYSRGNVTFSILTPGPNYRPGYNNFYNTPSLQEFVKATQIRFHFHGQYYTTETAVNLRHRYYAVDEITISGRCQCHGHADNCDTTSQPYRCLCSQESFTEGLHCDRCLPLYNDKPFRQGDQVYAFNCKPCQCNSHSKSCHYNISVDPFPFEHFRGGGGVCDDCEHNTTGRNCELCKDYFFRQVGADPSAIDVCKPCDCDTVGTRNGSILCDQIGGQCNCKRHVSGRQCNQCQNGFYNLQELDPDGCSPCNCNTSGTVDGDITCHQNSGQCKCKANVIGLRCDHCNFGFKFLRSFNDVGCEPCQCNLHGSVNKFCNPHSGQCECKKEAKGLQCDTCRENFYGLDVTNCKACDCDTAGSLPGTVCNAKTGQCICKPNVEGRQCNKCLEGNFYLRQNNSFLCLPCNCDKTGTINGSLLCNKSTGQCPCKLGVTGLRCNQCEPHRYNLTIDNFQHCQMCECDSLGTLPGTICDPISGQCLCVPNRQGRRCNQCQPGFYISPGNATGCLPCSCHTTGAVNHICNSLTGQCVCQDASIAGQRCDQCKDHYFGFDPQTGRCQPCNCHLSGALNETCHLVTGQCFCKQFVTGSKCDACVPSASHLDVNNLLGCSKTPFQQPPPRGQVQSSSAINLSWSPPDSPNAHWLTYSLLRDGFEIYTTEDQYPYSIQYFLDTDLLPYTKYSYYIETTNVHGSTRSVAVTYKTKPGVPEGNLTLSYIIPIGSDSVTLTWTTLSNQSGPIEKYILSCAPLAGGQPCVSYEGHETSATIWNLVPFAKYDFSVQACTSGGCLHSLPITVTTAQAPPQRLSPPKMQKISSTELHVEWSPPAELNGIIIRYELYMRRLRSTKETTSEESRVFQSSGWLSPHSFVESANENALKPPQTMTTITGLEPYTKYEFRVLAVNMAGSVSSAWVSERTGESAPVFMIPPSVFPLSSYSLNISWEKPADNVTRGKVVGYDINMLSEQSPQQSIPMAFSQLLHTAKSQELSYTVEGLKPYRIYEFTITLCNSVGCVTSASGAGQTLAAAPAQLRPPLVKGINSTTIHLKWFPPEELNGPSPIYQLERRESSLPALMTTMMKGIRFIGNGYCKFPSSTHPVNTDFTGIKASFRTKVPEGLIVFAASPGNQEEYFALQLKKGRLYFLFDPQGSPVEVTTTNDHGKQYSDGKWHEIIAIRHQAFGQITLDGIYTGSSAILNGSTVIGDNTGVFLGGLPRSYTILRKDPEIIQKGFVGCLKDVHFMKNYNPSAIWEPLDWQSSEEQINVYNSWEGCPASLNEGAQFLGAGFLELHPYMFHGGMNFEISFKFRTDQLNGLLLFVYNKDGPDFLAMELKSGILTFRLNTSLAFTQVDLLLGLSYCNGKWNKVIIKKEGSFISASVNGLMKHASESGDQPLVVNSPVYVGGIPQELLNSYQHLCLEQGFGGCMKDVKFTRGAVVNLASVSSGAVRVNLDGCLSTDSAVNCRGNDSILVYQGKEQSVYEGGLQPFTEYLYRVIASHEGGSVYSDWSRGRTTGAAPQSVPTPSRVRSLNGYSIEVTWDEPVVRGVIEKYILKAYSEDSTRPPRMPSASAEFVNTSNLTGILTGLLPFKNYAVTLTACTLAGCTESSHALNISTPQEAPQEVQPPVAKSLPSSLLLSWNPPKKANGIITQYCLYMDGRLIYSGSEENYTVTDLAVFTPHQFLLSACTHVGCTNSSWVLLYTAQLPPEHVDSPVLTVLDSRTIHIQWKQPRKISGILERYVLYMSNHTHDFTIWSVIYNSTELFQDHMLQYVLPGNKYLIKLGACTGGGCTVSEASEALTDEDIPEGVPAPKAHSYSPDSFNVSWTEPEYPNGVITSYGLYLDGILIHNSSELSYRAYGFAPWSLHSFRVQACTAKGCALGPLVENRTLEAPPEGTVNVFVKTQGSRKAHVRWEAPFRPNGLLTHSVLFTGIFYVDPVGNNYTLLNVTKVMYSGEETNLWVLIDGLVPFTNYTVQVNISNSQGSLITDPITIAMPPGAPDGVLPPRLSSATPTSLQVVWSTPARNNAPGSPRYQLQMRSGDSTHGFLELFSNPSASLSYEVSDLQPYTEYMFRLVASNGFGSAHSSWIPFMTAEDKPGPVVPPILLDVKSRMMLVTWQHPRKSNGVITHYNIYLHGRLYLRTPGNVTNCTVMHLHPYTAYKFQVEACTSKGCSLSPESQTVWTLPGAPEGIPSPELFSDTPTSVIISWQPPTHPNGLVENFTIERRVKGKEEVTTLVTLPRSHSMRFIDKTSALSPWTKYEYRVLMSTLHGGTNSSAWVEVTTRPSRPAGVQPPVVTVLEPDAVQVTWKPPLIQNGDILSYEIHMPDPHITLTNVTSAVLSQKVTHLIPFTNYSVTIVACSGGNGYLGGCTESLPTYVTTHPTVPQNVGPLSVIPLSESYVVISWQPPSKPNGPNLRYELLRRKIQQPLASNPPEDLNRWHNIYSGTQWLYEDKGLSRFTTYEYMLFVHNSVGFTPSREVTVTTLAGLPERGANLTASVLNHTAIDVRWAKPTVQDLQGEVEYYTLFWSSATSNDSLKILPDVNSHVIGHLKPNTEYWIFISVFNGVHSINSAGLHATTCDGEPQGMLPPEVVIINSTAVRVIWTSPSNPNGVVTEYSIYVNNKLYKTGMNVPGSFILRDLSPFTIYDIQVEVCTIYACVKSNGTQITTVEDTPSDIPTPTIRGITSRSLQIDWVSPRKPNGIILGYDLLWKTWYPCAKTQKLVQDQSDELCKAVRCQKPESICGHICYSSEAKVCCNGVLYNPKPGHRCCEEKYIPFVLNSTGVCCGGRIQEAQPNHQCCSGYYARILPGEVCCPDEQHNRVSVGIGDSCCGRMPYSTSGNQICCAGRLHDGHGQKCCGRQIVSNDLECCGGEEGVVYNRLPGMFCCGQDYVNMSDTICCSASSGESKAHIKKNDPVPVKCCETELIPKSQKCCNGVGYNPLKYVCSDKISTGMMMKETKECRILCPASMEATEHCGRCDFNFTSHICTVIRGSHNSTGKASIEEMCSSAEETIHTGSVNTYSYTDVNLKPYMTYEYRISAWNSYGRGLSKAVRARTKEDVPQGVSPPTWTKIDNLEDTIVLNWRKPIQSNGPIIYYILLRNGIERFRGTSLSFSDKEGIQPFQEYSYQLKACTVAGCATSSKVVAATTQGVPESILPPSITALSAVALHLSWSVPEKSNGVIKEYQIRQVGKGLIHTDTTDRRQHTVTGLQPYTNYSFTLTACTSAGCTSSEPFLGQTLQAAPEGVWVTPRHIIINSTTVELYWSLPEKPNGLVSQYQLSRNGNLLFLGGSEEQNFTDKNLEPNSRYTYKLEVKTGGGSSASDDYIVQTPMSTPEEIYPPYNITVIGPYSIFVAWIPPGILIPEIPVEYNVLLNDGSVTPLAFSVGHHQSTLLENLTPFTQYEIRIQACQNGSCGVSSRMFVKTPEAAPMDLNSPVLKALGSACIEIKWMPPEKPNGIIINYFIYRRPAGIEEESVLFVWSEGALEFMDEGDTLRPFTLYEYRVRACNSKGSVESLWSLTQTLEAPPQDFPAPWAQATSAHSVLLNWTKPESPNGIISHYRVVYQERPDDPTFNSPTVHAFTVKGTSHQAHLYGLEPFTTYRIGVVAANHAGEILSPWTLIQTLESSPSGLRNFIVEQKENGRALLLQWSEPMRTNGVIKTYNIFSDGFLEYSGLNRQFLFRRLDPFTLYTLTLEACTRAGCAHSAPQPLWTDEAPPDSQLAPTVHSVKSTSVELSWSEPVNPNGKIIRYEVIRRCFEGKAWGNQTIQADEKIVFTEYNTERNTFMYNDTGLQPWTQCEYKIYTWNSAGHTCSSWNVVRTLQAPPEGLSPPVISYVSMNPQKLLISWIPPEQSNGIIQSYRLQRNEMLYPFSFDPVTFNYTDEELLPFSTYSYALQACTSGGCSTSKPTSITTLEAAPSEVSPPDLWAVSATQMNVCWSPPTVQNGKITKYLVRYDNKESLAGQGLCLLVSHLQPYSQYNFSLVACTNGGCTASVSKSAWTMEALPENMDSPTLQVTGSESIEITWKPPRNPNGQIRSYELRRDGTIVYTGLETRYRDFTLTPGVEYSYTVTASNSQGGILSPLVKDRTSPSAPSGMEPPKLQARGPQEILVNWDPPVRTNGDIINYTLFIRELFERETKIIHINTTHNSFGMQSYIVNQLKPFHRYEIRIQACTTLGCASSDWTFIQTPEIAPLMQPPPHLEVQMAPGGFQPTVSLLWTGPLQPNGKVLYYELYRRQIATQPRKSNPVLIYNGSSTSFIDSELLPFTEYEYQVWAVNSAGKAPSSWTWCRTGPAPPEGLRAPTFHVISSTQAVVNISAPGKPNGIVSLYRLFSSSAHGAETVLSEGMATQQTLHGLQAFTNYSIGVEACTCFNCCSKGPTAELRTHPAPPSGLSSPQIGTLASRTASFRWSPPMFPNGVIHSYELQFHVACPPDSALPCTPSQIETKYTGLGQKASLGGLQPYTTYKLRVVAHNEVGSTASEWISFTTQKELPQYRAPFSVDSNLSVVCVNWSDTFLLNGQLKEYVLTDGGRRVYSGLDTTLYIPRTADKTFFFQVICTTDEGSVKTPLIQYDTSTGLGLVLTTPGKKKGSRSKSTEFYSELWFIVLMAMLGLILLAIFLSLILQRKIHKEPYIRERPPLVPLQKRMSPLNVYPPGENHMGLADTKIPRSGTPVSIRSNRSACVLRIPSQNQTSLTYSQGSLHRSVSQLMDIQDKKVLMDNSLWEAIMGHNSGLYVDEEDLMNAIKDFSSVTKERTTFTDTHL

Target ORF information:

RefSeq Version	NM_206933.3
Organism	Homo sapiens(human)
Definition	Homo sapiens usherin (USH2A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206933.3

ORF Insert Sequence:

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGCA TTAAGGCCAG CTTTCGAACA AAAGTGCCTG AAGGTTTGAT TGTCTTTGCA 
GCATCACCTG GCAATCAGGA AGAGTATTTT GCACTTCAGT TGAAGAAGGG ACGTCTTTAT 
TTTCTTTTTG ATCCTCAGGG GTCACCAGTG GAAGTAACTA CAACTAATGA TCATGGCAAA 
CAATATAGTG ATGGAAAATG GCATGAAATA ATTGCTATTA GGCATCAGGC TTTTGGCCAA 
ATCACTCTGG ATGGGATATA TACAGGTTCC TCTGCCATCC TGAATGGTAG TACTGTTATT 
GGAGATAACA CAGGAGTCTT TCTGGGAGGG CTCCCGCGAA GTTATACCAT CCTCAGGAAG 
GATCCTGAGA TAATCCAAAA AGGTTTTGTG GGCTGTCTCA AGGATGTACA TTTTATGAAG 
AATTACAATC CGTCAGCTAT TTGGGAACCT CTGGATTGGC AGAGTTCTGA AGAACAAATC 
AACGTGTATA ACAGCTGGGA GGGATGTCCC GCTTCATTAA ATGAGGGAGC TCAGTTCCTA 
GGAGCAGGGT TCCTGGAACT TCATCCATAT ATGTTTCATG GTGGAATGAA CTTTGAGATT 
TCCTTTAAGT TCAGAACTGA CCAATTAAAT GGATTGCTTC TTTTCGTTTA TAACAAAGAT 
GGACCTGATT TTCTTGCTAT GGAGCTGAAA AGTGGAATAT TGACCTTCCG GTTAAATACC 
AGTCTTGCCT TTACACAAGT GGATCTATTG CTGGGGCTAT CCTATTGTAA TGGAAAGTGG 
AATAAAGTCA TTATTAAAAA GGAAGGCTCT TTCATATCAG CAAGTGTGAA TGGACTGATG 
AAGCATGCAT CGGAGTCCGG AGACCAGCCA CTGGTGGTGA ATTCACCAGT TTATGTGGGA 
GGAATCCCAC AGGAACTGCT GAACTCTTAT CAACATTTGT GTTTGGAACA AGGTTTCGGT 
GGTTGCATGA AGGATGTTAA ATTTACACGG GGTGCTGTCG TTAACTTGGC ATCTGTGTCC 
AGCGGTGCTG TCAGAGTCAA TCTGGATGGA TGCCTATCAA CTGACAGTGC TGTTAACTGC 
AGGGGAAATG ACTCCATCCT GGTTTACCAG GGAAAAGAGC AGAGTGTTTA CGAGGGTGGT 
CTCCAGCCTT TTACAGAATA CCTGTATCGA GTGATAGCCT CGCATGAAGG AGGTTCAGTA 
TATAGTGATT GGAGTCGAGG ACGTACAACA GGAGCAGCTC CACAAAGTGT GCCAACTCCC 
TCAAGAGTCC GCAGCTTAAA TGGATACAGC ATTGAGGTGA CCTGGGATGA ACCTGTTGTC 
AGAGGTGTAA TTGAGAAGTA CATTCTGAAA GCCTATAGTG AGGACAGCAC CCGTCCACCC 
CGCATGCCCT CTGCCAGTGC TGAATTTGTC AATACAAGCA ACCTCACAGG CATATTGACA 
GGCTTGCTAC CCTTCAAAAA CTATGCAGTA ACCCTAACTG CTTGCACTTT GGCTGGCTGT 
ACTGAGAGCT CACATGCATT GAACATCTCT ACTCCACAAG AAGCCCCACA AGAGGTTCAG 
CCACCAGTAG CCAAATCCCT TCCCAGTTCT TTGCTGCTCT CCTGGAACCC ACCCAAAAAG 
GCAAATGGTA TTATAACTCA GTACTGTTTA TACATGGATG GGAGGCTGAT CTATTCAGGC 
AGTGAGGAGA ACTACACAGT CACAGATTTA GCAGTATTTA CACCCCACCA GTTTCTACTA 
AGTGCATGCA CACATGTGGG CTGTACAAAC AGTTCCTGGG TCCTACTGTA CACAGCACAG 
CTGCCACCAG AACACGTGGA TTCCCCAGTT CTGACTGTCC TGGATTCTAG AACTATACAC 
ATACAGTGGA AACAACCAAG AAAAATAAGT GGGATTCTGG AACGCTATGT ATTATATATG 
TCAAACCATA CACATGATTT TACAATTTGG AGTGTCATCT ATAACAGTAC AGAACTTTTC 
CAGGATCATA TGCTACAATA CGTTTTACCT GGTAATAAAT ATCTCATCAA GCTGGGAGCT 
TGCACAGGTG GTGGGTGCAC AGTGAGTGAG GCCAGTGAGG CCCTAACTGA CGAGGACATA 
CCCGAAGGCG TGCCAGCCCC CAAAGCCCAC TCATATTCAC CTGACTCCTT TAATGTCTCC 
TGGACTGAGC CTGAATATCC GAATGGTGTT ATCACGAGTT ATGGATTATA TCTAGATGGT 
ATATTAATCC ACAATTCCTC AGAACTCAGC TATCGTGCTT ACGGATTTGC TCCTTGGAGT 
TTACATTCCT TCAGAGTCCA AGCATGCACG GCCAAAGGTT GTGCTCTGGG CCCACTGGTG 
GAAAATCGAA CTCTAGAAGC TCCTCCTGAA GGAACAGTAA ATGTGTTTGT CAAAACACAG 
GGATCCCGGA AAGCCCACGT GAGGTGGGAA GCACCTTTTC GCCCTAATGG ACTCTTAACA 
CACTCAGTCC TTTTCACTGG GATATTCTAT GTAGACCCAG TAGGTAATAA CTACACCCTT 
CTGAATGTCA CAAAAGTCAT GTACAGCGGA GAAGAGACAA ACCTTTGGGT GCTCATCGAT 
GGGCTGGTTC CTTTTACCAA CTATACTGTA CAAGTGAATA TTTCAAATAG CCAAGGCAGC 
TTGATAACTG ATCCTATAAC AATTGCAATG CCTCCAGGAG CTCCAGATGG CGTGCTGCCT 
CCCAGGCTTT CATCTGCCAC TCCAACCAGT CTTCAGGTTG TCTGGTCTAC ACCAGCTCGT 
AATAACGCTC CTGGCTCTCC CAGATACCAA CTCCAGATGA GGTCTGGCGA CTCCACCCAT 
GGATTTCTAG AGTTATTTTC CAATCCTTCT GCATCGTTAA GCTATGAAGT GAGTGATCTC 
CAACCGTACA CAGAGTATAT GTTTCGGTTG GTTGCCTCCA ATGGATTTGG CAGTGCACAT 
AGTTCTTGGA TTCCATTCAT GACCGCAGAG GACAAACCTG GACCTGTAGT TCCTCCGATT 
CTTCTGGATG TGAAGTCAAG AATGATGTTG GTCACCTGGC AGCATCCTAG AAAATCCAAT 
GGGGTTATTA CCCATTATAA CATTTATCTA CATGGCCGTC TATACTTGAG AACTCCTGGA 
AATGTCACTA ATTGCACAGT GATGCATTTA CACCCATACA CTGCCTATAA GTTTCAGGTA 
GAAGCCTGCA CTTCAAAAGG ATGTTCCCTT TCACCAGAGT CCCAGACTGT ATGGACACTC 
CCAGGGGCAC CGGAAGGGAT CCCAAGTCCA GAGCTGTTCT CTGATACTCC AACATCTGTG 
ATTATATCTT GGCAACCCCC TACCCACCCC AATGGCTTGG TGGAGAATTT CACAATTGAG 
AGAAGAGTCA AAGGAAAGGA AGAAGTTACT ACCCTGGTGA CTCTCCCGAG GAGTCATTCC 
ATGAGGTTTA TTGACAAGAC TTCTGCTCTT AGCCCATGGA CAAAATATGA ATATCGGGTA 
CTGATGAGCA CTCTTCATGG AGGCACAAAC AGCAGTGCTT GGGTAGAAGT TACCACAAGA 
CCCTCACGAC CTGCTGGGGT GCAGCCACCT GTGGTGACAG TGCTGGAACC CGATGCAGTC 
CAGGTCACTT GGAAACCCCC ACTCATCCAG AACGGAGACA TACTTAGCTA TGAGATTCAC 
ATGCCTGACC CTCACATCAC TTTAACCAAT GTGACTTCCG CAGTGTTAAG TCAAAAAGTT 
ACTCATCTGA TTCCTTTCAC TAATTATTCT GTCACCATTG TTGCTTGCTC AGGGGGTAAT 
GGGTACCTTG GAGGGTGCAC AGAGAGTTTA CCTACCTATG TTACCACTCA CCCCACCGTA 
CCTCAGAATG TTGGCCCATT GTCTGTGATT CCACTAAGTG AATCATATGT TGTGATTTCT 
TGGCAACCAC CATCCAAGCC AAATGGACCT AATTTGAGAT ATGAGCTTCT GAGACGTAAA 
ATCCAGCAGC CACTTGCATC AAATCCCCCA GAAGATTTAA ATCGGTGGCA CAATATTTAT 
TCAGGAACTC AGTGGCTTTA TGAAGATAAG GGTCTTAGCA GGTTTACAAC CTATGAATAT 
ATGCTCTTCG TACACAACAG TGTGGGTTTT ACACCGAGCC GAGAAGTGAC TGTGACAACG 
TTAGCTGGTC TTCCAGAGAG AGGAGCCAAT CTCACTGCGA GTGTCCTTAA CCACACAGCC 
ATCGACGTGA GGTGGGCTAA ACCAACTGTT CAAGACCTAC AAGGTGAAGT TGAATATTAC 
ACACTTTTTT GGAGTTCTGC TACCTCAAAC GACTCTCTAA AAATCTTGCC AGATGTAAAC 
TCTCATGTCA TTGGCCACCT AAAGCCAAAC ACAGAGTATT GGATCTTTAT CTCTGTCTTC 
AATGGAGTCC ACAGCATCAA CAGTGCAGGA CTTCATGCAA CCACTTGCGA TGGGGAGCCT 
CAGGGCATGC TTCCTCCAGA GGTTGTCATC ATCAACAGTA CAGCTGTACG TGTCATCTGG 
ACATCTCCTT CAAACCCAAA TGGTGTTGTC ACTGAGTATT CTATCTATGT AAATAATAAG 
CTCTACAAGA CTGGAATGAA TGTGCCTGGG TCGTTTATTC TGAGAGACCT GTCTCCCTTC 
ACTATCTATG ACATTCAGGT TGAAGTCTGC ACAATATATG CCTGCGTGAA AAGCAATGGA 
ACCCAAATTA CCACTGTGGA AGACACTCCA AGTGATATAC CAACACCCAC AATTCGTGGC 
ATCACTTCAA GATCTCTTCA AATTGATTGG GTGTCTCCAC GGAAGCCAAA TGGCATCATT 
CTTGGATATG ATCTCCTATG GAAAACATGG TATCCATGCG CTAAAACTCA AAAGTTAGTG 
CAGGATCAGA GTGATGAGCT CTGCAAGGCA GTGAGGTGTC AAAAACCTGA ATCTATCTGT 
GGACACATTT GCTATTCTTC TGAAGCTAAG GTTTGTTGTA ACGGAGTGCT CTATAACCCC 
AAGCCTGGAC ATCGCTGTTG TGAAGAAAAG TATATCCCGT TTGTTCTGAA TTCTACTGGA 
GTTTGTTGTG GTGGCCGAAT ACAGGAGGCA CAACCAAATC ATCAGTGCTG CTCTGGGTAT 
TACGCTAGAA TTCTACCAGG TGAAGTATGC TGTCCAGATG AACAGCACAA TCGGGTTTCT 
GTTGGCATTG GTGATTCCTG CTGTGGCAGA ATGCCGTACT CCACCTCAGG AAACCAGATT 
TGCTGTGCTG GGAGGCTTCA TGATGGCCAT GGCCAGAAGT GCTGTGGCAG ACAGATTGTG 
AGCAACGATT TAGAGTGTTG TGGTGGAGAA GAAGGAGTGG TGTACAATCG CCTTCCAGGT 
ATGTTCTGTT GTGGGCAGGA TTATGTGAAT ATGTCAGATA CCATATGCTG CTCAGCTTCC 
AGTGGAGAGT CTAAAGCACA TATTAAAAAG AATGACCCGG TGCCAGTAAA ATGCTGTGAG 
ACTGAACTTA TTCCAAAGAG CCAGAAATGC TGTAATGGAG TTGGATATAA TCCTTTGAAA 
TATGTTTGCT CTGACAAGAT TTCAACTGGA ATGATGATGA AGGAAACCAA AGAGTGCAGG 
ATCCTCTGCC CAGCATCTAT GGAAGCCACA GAACATTGTG GCAGGTGTGA CTTCAACTTT 
ACCAGCCACA TTTGCACTGT GATAAGAGGG TCTCACAATT CCACAGGGAA GGCATCAATT 
GAAGAAATGT GTTCATCTGC CGAAGAAACC ATTCATACAG GGAGTGTAAA CACGTACTCT 
TACACAGATG TGAACCTCAA GCCCTACATG ACATATGAGT ACAGGATTTC TGCCTGGAAC 
AGCTATGGGC GAGGACTCAG CAAAGCTGTG AGAGCCAGAA CAAAAGAAGA TGTGCCTCAA 
GGAGTGAGTC CCCCTACGTG GACCAAAATA GACAATCTTG AAGATACAAT TGTCTTAAAC 
TGGAGAAAAC CTATACAATC AAATGGTCCT ATTATTTACT ACATCCTTCT TCGAAATGGA 
ATTGAACGTT TTCGGGGAAC ATCACTGAGC TTCTCTGATA AAGAGGGAAT TCAACCATTT 
CAGGAATATT CATATCAGCT GAAAGCTTGC ACGGTTGCTG GCTGTGCCAC CAGTAGCAAG 
GTAGTTGCAG CTACTACCCA AGGAGTTCCG GAGAGCATCC TGCCACCAAG CATCACAGCC 
CTAAGTGCAG TGGCTCTGCA TCTGAGCTGG AGTGTCCCTG AGAAATCAAA CGGCGTCATT 
AAAGAGTACC AGATCAGGCA GGTTGGGAAA GGTCTCATCC ACACTGACAC CACTGACAGG 
AGACAGCATA CGGTCACAGG TCTCCAGCCA TACACCAACT ACAGCTTCAC TCTTACAGCT 
TGTACATCTG CTGGGTGCAC TTCAAGCGAG CCTTTTCTAG GTCAGACACT GCAGGCAGCT 
CCTGAAGGAG TTTGGGTGAC ACCTCGACAC ATTATCATCA ATTCTACAAC AGTGGAATTA 
TATTGGAGTC TGCCAGAAAA GCCCAATGGC CTCGTTTCTC AATATCAATT GAGTCGTAAT 
GGAAACTTGC TTTTCCTGGG TGGCAGTGAG GAGCAGAATT TCACTGATAA AAACCTGGAG 
CCCAATAGCA GATACACTTA CAAGTTAGAA GTCAAAACTG GAGGTGGCAG CAGTGCTAGT 
GATGATTACA TTGTTCAAAC ACCTATGTCA ACACCAGAAG AAATCTATCC TCCATATAAT 
ATCACAGTAA TTGGGCCTTA TTCTATATTT GTAGCTTGGA TACCACCAGG GATCCTCATC 
CCCGAAATTC CTGTGGAGTA CAATGTCTTA CTCAATGATG GAAGTGTAAC ACCTCTGGCC 
TTCTCCGTTG GTCATCATCA ATCCACCCTT CTGGAAAATT TGACTCCATT CACACAGTAT 
GAGATAAGGA TACAAGCATG TCAAAATGGA AGTTGTGGAG TTAGCAGTAG GATGTTTGTC 
AAAACACCTG AAGCAGCCCC AATGGATCTT AATTCTCCTG TTCTTAAGGC ACTGGGGTCA 
GCTTGCATAG AGATTAAGTG GATGCCACCT GAAAAACCAA ATGGAATCAT CATCAACTAC 
TTTATTTACA GACGCCCTGC TGGCATTGAA GAGGAGTCTG TTTTATTTGT CTGGTCAGAA 
GGAGCCCTTG AATTTATGGA TGAAGGAGAC ACCCTGAGGC CTTTCACACT CTACGAATAT 
CGGGTCAGAG CCTGTAACTC CAAGGGTTCA GTGGAGAGTC TGTGGTCATT AACACAAACT 
CTGGAAGCTC CACCTCAAGA TTTTCCAGCT CCTTGGGCTC AAGCCACGAG TGCTCATTCA 
GTTCTGTTGA ATTGGACAAA GCCAGAATCT CCCAATGGCA TTATCTCCCA TTACCGTGTG 
GTCTACCAGG AGAGACCCGA CGATCCTACA TTTAACAGCC CTACCGTGCA TGCTTTCACA 
GTGAAGGGAA CAAGCCATCA AGCCCACCTG TACGGGTTAG AACCATTCAC AACATATCGC 
ATTGGTGTTG TGGCTGCAAA CCATGCAGGA GAAATTTTAA GCCCTTGGAC TCTGATTCAA 
ACCTTAGAAT CTTCCCCAAG TGGACTGAGA AACTTTATAG TAGAACAGAA AGAGAATGGC 
CGGGCATTGC TACTACAGTG GTCAGAACCT ATGAGAACCA ATGGTGTGAT TAAGACATAC 
AACATCTTCA GTGACGGGTT CCTGGAGTAC TCTGGTTTGA ATCGTCAGTT TCTCTTCCGC 
CGCCTGGATC CTTTCACTCT CTACACACTG ACCCTGGAGG CCTGCACCAG AGCAGGTTGT 
GCACACTCGG CGCCTCAGCC TCTGTGGACA GATGAAGCCC CTCCAGACTC TCAGCTGGCT 
CCTACTGTCC ACTCTGTGAA GTCCACCAGT GTTGAGCTGA GCTGGTCTGA GCCTGTTAAC 
CCAAATGGAA AAATAATTCG CTATGAAGTG ATTCGCAGAT GCTTCGAGGG AAAAGCTTGG 
GGAAATCAGA CGATCCAGGC CGACGAGAAA ATTGTTTTCA CAGAATATAA CACTGAAAGG 
AATACATTTA TGTATAATGA CACAGGTTTG CAACCATGGA CGCAGTGTGA ATATAAAATC 
TACACTTGGA ATTCAGCTGG GCATACCTGT AGCTCTTGGA ATGTGGTGAG GACATTGCAA 
GCACCTCCAG AAGGTCTCTC TCCACCTGTG ATATCCTATG TTTCTATGAA TCCCCAAAAA 
CTGCTGATTT CCTGGATCCC ACCAGAACAG TCTAATGGTA TTATCCAGTC CTATAGGCTT 
CAAAGGAATG AAATGCTCTA TCCTTTTAGC TTTGATCCTG TGACTTTCAA TTACACTGAT 
GAAGAGCTTC TTCCTTTTTC CACCTATAGC TATGCACTCC AAGCCTGCAC GAGTGGAGGA 
TGCTCCACCA GCAAACCCAC CAGCATCACA ACTCTGGAGG CTGCTCCATC AGAAGTCAGC 
CCTCCAGATC TTTGGGCCGT CAGTGCCACT CAAATGAATG TATGTTGGTC ACCGCCCACA 
GTGCAAAATG GAAAGATTAC TAAATATTTA GTTAGATATG ATAATAAAGA GTCCCTTGCT 
GGCCAGGGCC TGTGCCTGCT GGTTTCCCAC CTGCAGCCTT ACTCTCAGTA TAACTTCTCC 
CTTGTAGCCT GCACGAATGG AGGTTGCACA GCTAGTGTGT CAAAATCTGC CTGGACAATG 
GAGGCCCTGC CAGAGAACAT GGACTCTCCA ACATTGCAAG TCACAGGCTC AGAATCAATA 
GAAATCACCT GGAAACCTCC AAGAAACCCA AATGGCCAGA TCAGAAGTTA TGAACTTAGG 
AGGGATGGAA CCATTGTATA TACAGGCTTG GAAACACGCT ATCGTGATTT TACTCTCACC 
CCAGGTGTGG AGTATAGCTA CACAGTAACT GCCAGCAACA GCCAAGGGGG TATTTTGAGT 
CCTCTTGTCA AAGATCGAAC CAGCCCCTCA GCACCCTCAG GGATGGAACC TCCAAAATTG 
CAGGCCAGGG GTCCTCAGGA GATCTTAGTG AACTGGGACC CTCCAGTGAG AACAAATGGT 
GATATCATCA ATTATACCCT CTTCATCCGT GAACTATTTG AAAGAGAAAC TAAAATCATA 
CACATAAACA CAACTCATAA TTCTTTTGGT ATGCAGTCAT ATATAGTAAA CCAGCTGAAG 
CCATTTCACA GGTATGAAAT ACGAATTCAA GCGTGCACCA CCCTGGGATG TGCATCAAGT 
GACTGGACAT TCATACAGAC CCCTGAGATT GCACCTTTGA TGCAACCCCC TCCACATCTG 
GAGGTACAAA TGGCTCCAGG AGGATTCCAG CCAACTGTTT CTCTTTTGTG GACAGGACCG 
CTGCAGCCAA ATGGAAAAGT TTTGTATTAC GAATTATACA GAAGACAAAT AGCAACTCAG 
CCTAGAAAAT CCAATCCAGT CCTAATCTAT AACGGAAGCT CAACATCTTT TATAGATTCC 
GAACTATTGC CTTTCACAGA GTATGAGTAT CAGGTCTGGG CAGTGAATTC TGCAGGAAAA 
GCCCCCAGTA GCTGGACATG GTGCAGAACC GGGCCAGCCC CACCAGAAGG TCTCAGAGCC 
CCCACGTTCC ATGTGATCTC TTCTACCCAA GCAGTGGTCA ACATCAGTGC CCCTGGGAAG 
CCCAACGGGA TCGTCAGTCT CTACAGGCTG TTCTCCAGCA GCGCCCATGG GGCTGAGACA 
GTGCTATCCG AAGGCATGGC CACCCAGCAG ACTCTCCATG GCCTTCAAGC CTTCACTAAC 
TACTCTATTG GAGTAGAGGC CTGCACCTGC TTCAACTGTT GCAGCAAAGG ACCGACAGCT 
GAACTGAGAA CCCATCCTGC CCCACCCTCA GGACTGTCCT CTCCACAAAT CGGGACGCTG 
GCCTCAAGGA CGGCCTCCTT CCGGTGGAGT CCCCCCATGT TCCCCAATGG TGTCATTCAC 
AGCTATGAAC TCCAATTCCA CGTGGCTTGC CCTCCTGACT CAGCCCTCCC CTGTACTCCC 
AGCCAAATAG AAACAAAGTA CACGGGGCTG GGGCAGAAAG CCAGCCTTGG GGGTCTCCAG 
CCCTACACCA CATACAAGCT GAGAGTGGTG GCACACAACG AGGTGGGCAG TACGGCTTCC 
GAGTGGATCA GTTTCACCAC CCAAAAAGAA TTGCCTCAGT ACCGAGCCCC ATTTTCGGTG 
GACAGCAATT TGTCTGTGGT GTGTGTGAAC TGGAGTGACA CCTTCCTCCT GAACGGCCAA 
CTGAAGGAGT ACGTGTTAAC CGACGGAGGG CGACGCGTGT ACAGCGGCTT GGACACCACC 
CTCTACATAC CGAGAACGGC GGACAAAACC TTCTTTTTCC AGGTCATCTG CACGACTGAC 
GAAGGAAGTG TTAAGACGCC GTTGATCCAA TATGATACCT CTACTGGACT TGGCTTGGTC 
CTAACAACTC CTGGGAAAAA GAAGGGATCG CGGAGCAAAA GCACAGAGTT CTACAGCGAG 
CTGTGGTTCA TAGTGTTAAT GGCGATGCTG GGCTTGATCT TGTTGGCCAT TTTTCTGTCC 
CTGATACTAC AAAGAAAAAT CCACAAAGAG CCATATATCA GAGAAAGACC TCCCTTGGTA 
CCTCTTCAGA AGAGGATGTC TCCATTGAAT GTTTACCCAC CGGGGGAAAA CCATATGGGG 
TTAGCCGATA CCAAAATTCC CCGGTCTGGG ACACCTGTGA GTATCCGCAG CAACCGGAGT 
GCATGTGTCC TGCGCATCCC GAGTCAAAAC CAAACCAGCC TAACCTACTC CCAGGGTTCT 
CTTCACCGCA GCGTCAGCCA GCTCATGGAC ATTCAAGACA AGAAAGTCTT GATGGACAAC 
TCACTGTGGG AAGCCATCAT GGGCCACAAC AGTGGACTGT ATGTGGATGA AGAGGACCTG 
ATGAACGCCA TCAAGGATTT CAGCTCAGTG ACTAAGGAAC GCACCACATT CACAGACACC 
CACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USH2A ( NM_206933.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_996816.2 Homo sapiens usherin (USH2A), transcript variant 2, mRNA.

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CloneID	OHu19218
Clone ID Related Accession (Same CDS sequence)	NM_206933.3 , NM_206933.2
Accession Version	NM_206933.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	usherin isoform B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY481573.1, AF055580.2, AA883599.1 and AADB02001158.1. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2009 this sequence version replaced NM_206933.1. Transcript Variant: This variant (2) is the longer transcript and it encodes the longer isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY481573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGCA TTAAGGCCAG CTTTCGAACA AAAGTGCCTG AAGGTTTGAT TGTCTTTGCA 
GCATCACCTG GCAATCAGGA AGAGTATTTT GCACTTCAGT TGAAGAAGGG ACGTCTTTAT 
TTTCTTTTTG ATCCTCAGGG GTCACCAGTG GAAGTAACTA CAACTAATGA TCATGGCAAA 
CAATATAGTG ATGGAAAATG GCATGAAATA ATTGCTATTA GGCATCAGGC TTTTGGCCAA 
ATCACTCTGG ATGGGATATA TACAGGTTCC TCTGCCATCC TGAATGGTAG TACTGTTATT 
GGAGATAACA CAGGAGTCTT TCTGGGAGGG CTCCCGCGAA GTTATACCAT CCTCAGGAAG 
GATCCTGAGA TAATCCAAAA AGGTTTTGTG GGCTGTCTCA AGGATGTACA TTTTATGAAG 
AATTACAATC CGTCAGCTAT TTGGGAACCT CTGGATTGGC AGAGTTCTGA AGAACAAATC 
AACGTGTATA ACAGCTGGGA GGGATGTCCC GCTTCATTAA ATGAGGGAGC TCAGTTCCTA 
GGAGCAGGGT TCCTGGAACT TCATCCATAT ATGTTTCATG GTGGAATGAA CTTTGAGATT 
TCCTTTAAGT TCAGAACTGA CCAATTAAAT GGATTGCTTC TTTTCGTTTA TAACAAAGAT 
GGACCTGATT TTCTTGCTAT GGAGCTGAAA AGTGGAATAT TGACCTTCCG GTTAAATACC 
AGTCTTGCCT TTACACAAGT GGATCTATTG CTGGGGCTAT CCTATTGTAA TGGAAAGTGG 
AATAAAGTCA TTATTAAAAA GGAAGGCTCT TTCATATCAG CAAGTGTGAA TGGACTGATG 
AAGCATGCAT CGGAGTCCGG AGACCAGCCA CTGGTGGTGA ATTCACCAGT TTATGTGGGA 
GGAATCCCAC AGGAACTGCT GAACTCTTAT CAACATTTGT GTTTGGAACA AGGTTTCGGT 
GGTTGCATGA AGGATGTTAA ATTTACACGG GGTGCTGTCG TTAACTTGGC ATCTGTGTCC 
AGCGGTGCTG TCAGAGTCAA TCTGGATGGA TGCCTATCAA CTGACAGTGC TGTTAACTGC 
AGGGGAAATG ACTCCATCCT GGTTTACCAG GGAAAAGAGC AGAGTGTTTA CGAGGGTGGT 
CTCCAGCCTT TTACAGAATA CCTGTATCGA GTGATAGCCT CGCATGAAGG AGGTTCAGTA 
TATAGTGATT GGAGTCGAGG ACGTACAACA GGAGCAGCTC CACAAAGTGT GCCAACTCCC 
TCAAGAGTCC GCAGCTTAAA TGGATACAGC ATTGAGGTGA CCTGGGATGA ACCTGTTGTC 
AGAGGTGTAA TTGAGAAGTA CATTCTGAAA GCCTATAGTG AGGACAGCAC CCGTCCACCC 
CGCATGCCCT CTGCCAGTGC TGAATTTGTC AATACAAGCA ACCTCACAGG CATATTGACA 
GGCTTGCTAC CCTTCAAAAA CTATGCAGTA ACCCTAACTG CTTGCACTTT GGCTGGCTGT 
ACTGAGAGCT CACATGCATT GAACATCTCT ACTCCACAAG AAGCCCCACA AGAGGTTCAG 
CCACCAGTAG CCAAATCCCT TCCCAGTTCT TTGCTGCTCT CCTGGAACCC ACCCAAAAAG 
GCAAATGGTA TTATAACTCA GTACTGTTTA TACATGGATG GGAGGCTGAT CTATTCAGGC 
AGTGAGGAGA ACTACACAGT CACAGATTTA GCAGTATTTA CACCCCACCA GTTTCTACTA 
AGTGCATGCA CACATGTGGG CTGTACAAAC AGTTCCTGGG TCCTACTGTA CACAGCACAG 
CTGCCACCAG AACACGTGGA TTCCCCAGTT CTGACTGTCC TGGATTCTAG AACTATACAC 
ATACAGTGGA AACAACCAAG AAAAATAAGT GGGATTCTGG AACGCTATGT ATTATATATG 
TCAAACCATA CACATGATTT TACAATTTGG AGTGTCATCT ATAACAGTAC AGAACTTTTC 
CAGGATCATA TGCTACAATA CGTTTTACCT GGTAATAAAT ATCTCATCAA GCTGGGAGCT 
TGCACAGGTG GTGGGTGCAC AGTGAGTGAG GCCAGTGAGG CCCTAACTGA CGAGGACATA 
CCCGAAGGCG TGCCAGCCCC CAAAGCCCAC TCATATTCAC CTGACTCCTT TAATGTCTCC 
TGGACTGAGC CTGAATATCC GAATGGTGTT ATCACGAGTT ATGGATTATA TCTAGATGGT 
ATATTAATCC ACAATTCCTC AGAACTCAGC TATCGTGCTT ACGGATTTGC TCCTTGGAGT 
TTACATTCCT TCAGAGTCCA AGCATGCACG GCCAAAGGTT GTGCTCTGGG CCCACTGGTG 
GAAAATCGAA CTCTAGAAGC TCCTCCTGAA GGAACAGTAA ATGTGTTTGT CAAAACACAG 
GGATCCCGGA AAGCCCACGT GAGGTGGGAA GCACCTTTTC GCCCTAATGG ACTCTTAACA 
CACTCAGTCC TTTTCACTGG GATATTCTAT GTAGACCCAG TAGGTAATAA CTACACCCTT 
CTGAATGTCA CAAAAGTCAT GTACAGCGGA GAAGAGACAA ACCTTTGGGT GCTCATCGAT 
GGGCTGGTTC CTTTTACCAA CTATACTGTA CAAGTGAATA TTTCAAATAG CCAAGGCAGC 
TTGATAACTG ATCCTATAAC AATTGCAATG CCTCCAGGAG CTCCAGATGG CGTGCTGCCT 
CCCAGGCTTT CATCTGCCAC TCCAACCAGT CTTCAGGTTG TCTGGTCTAC ACCAGCTCGT 
AATAACGCTC CTGGCTCTCC CAGATACCAA CTCCAGATGA GGTCTGGCGA CTCCACCCAT 
GGATTTCTAG AGTTATTTTC CAATCCTTCT GCATCGTTAA GCTATGAAGT GAGTGATCTC 
CAACCGTACA CAGAGTATAT GTTTCGGTTG GTTGCCTCCA ATGGATTTGG CAGTGCACAT 
AGTTCTTGGA TTCCATTCAT GACCGCAGAG GACAAACCTG GACCTGTAGT TCCTCCGATT 
CTTCTGGATG TGAAGTCAAG AATGATGTTG GTCACCTGGC AGCATCCTAG AAAATCCAAT 
GGGGTTATTA CCCATTATAA CATTTATCTA CATGGCCGTC TATACTTGAG AACTCCTGGA 
AATGTCACTA ATTGCACAGT GATGCATTTA CACCCATACA CTGCCTATAA GTTTCAGGTA 
GAAGCCTGCA CTTCAAAAGG ATGTTCCCTT TCACCAGAGT CCCAGACTGT ATGGACACTC 
CCAGGGGCAC CGGAAGGGAT CCCAAGTCCA GAGCTGTTCT CTGATACTCC AACATCTGTG 
ATTATATCTT GGCAACCCCC TACCCACCCC AATGGCTTGG TGGAGAATTT CACAATTGAG 
AGAAGAGTCA AAGGAAAGGA AGAAGTTACT ACCCTGGTGA CTCTCCCGAG GAGTCATTCC 
ATGAGGTTTA TTGACAAGAC TTCTGCTCTT AGCCCATGGA CAAAATATGA ATATCGGGTA 
CTGATGAGCA CTCTTCATGG AGGCACAAAC AGCAGTGCTT GGGTAGAAGT TACCACAAGA 
CCCTCACGAC CTGCTGGGGT GCAGCCACCT GTGGTGACAG TGCTGGAACC CGATGCAGTC 
CAGGTCACTT GGAAACCCCC ACTCATCCAG AACGGAGACA TACTTAGCTA TGAGATTCAC 
ATGCCTGACC CTCACATCAC TTTAACCAAT GTGACTTCCG CAGTGTTAAG TCAAAAAGTT 
ACTCATCTGA TTCCTTTCAC TAATTATTCT GTCACCATTG TTGCTTGCTC AGGGGGTAAT 
GGGTACCTTG GAGGGTGCAC AGAGAGTTTA CCTACCTATG TTACCACTCA CCCCACCGTA 
CCTCAGAATG TTGGCCCATT GTCTGTGATT CCACTAAGTG AATCATATGT TGTGATTTCT 
TGGCAACCAC CATCCAAGCC AAATGGACCT AATTTGAGAT ATGAGCTTCT GAGACGTAAA 
ATCCAGCAGC CACTTGCATC AAATCCCCCA GAAGATTTAA ATCGGTGGCA CAATATTTAT 
TCAGGAACTC AGTGGCTTTA TGAAGATAAG GGTCTTAGCA GGTTTACAAC CTATGAATAT 
ATGCTCTTCG TACACAACAG TGTGGGTTTT ACACCGAGCC GAGAAGTGAC TGTGACAACG 
TTAGCTGGTC TTCCAGAGAG AGGAGCCAAT CTCACTGCGA GTGTCCTTAA CCACACAGCC 
ATCGACGTGA GGTGGGCTAA ACCAACTGTT CAAGACCTAC AAGGTGAAGT TGAATATTAC 
ACACTTTTTT GGAGTTCTGC TACCTCAAAC GACTCTCTAA AAATCTTGCC AGATGTAAAC 
TCTCATGTCA TTGGCCACCT AAAGCCAAAC ACAGAGTATT GGATCTTTAT CTCTGTCTTC 
AATGGAGTCC ACAGCATCAA CAGTGCAGGA CTTCATGCAA CCACTTGCGA TGGGGAGCCT 
CAGGGCATGC TTCCTCCAGA GGTTGTCATC ATCAACAGTA CAGCTGTACG TGTCATCTGG 
ACATCTCCTT CAAACCCAAA TGGTGTTGTC ACTGAGTATT CTATCTATGT AAATAATAAG 
CTCTACAAGA CTGGAATGAA TGTGCCTGGG TCGTTTATTC TGAGAGACCT GTCTCCCTTC 
ACTATCTATG ACATTCAGGT TGAAGTCTGC ACAATATATG CCTGCGTGAA AAGCAATGGA 
ACCCAAATTA CCACTGTGGA AGACACTCCA AGTGATATAC CAACACCCAC AATTCGTGGC 
ATCACTTCAA GATCTCTTCA AATTGATTGG GTGTCTCCAC GGAAGCCAAA TGGCATCATT 
CTTGGATATG ATCTCCTATG GAAAACATGG TATCCATGCG CTAAAACTCA AAAGTTAGTG 
CAGGATCAGA GTGATGAGCT CTGCAAGGCA GTGAGGTGTC AAAAACCTGA ATCTATCTGT 
GGACACATTT GCTATTCTTC TGAAGCTAAG GTTTGTTGTA ACGGAGTGCT CTATAACCCC 
AAGCCTGGAC ATCGCTGTTG TGAAGAAAAG TATATCCCGT TTGTTCTGAA TTCTACTGGA 
GTTTGTTGTG GTGGCCGAAT ACAGGAGGCA CAACCAAATC ATCAGTGCTG CTCTGGGTAT 
TACGCTAGAA TTCTACCAGG TGAAGTATGC TGTCCAGATG AACAGCACAA TCGGGTTTCT 
GTTGGCATTG GTGATTCCTG CTGTGGCAGA ATGCCGTACT CCACCTCAGG AAACCAGATT 
TGCTGTGCTG GGAGGCTTCA TGATGGCCAT GGCCAGAAGT GCTGTGGCAG ACAGATTGTG 
AGCAACGATT TAGAGTGTTG TGGTGGAGAA GAAGGAGTGG TGTACAATCG CCTTCCAGGT 
ATGTTCTGTT GTGGGCAGGA TTATGTGAAT ATGTCAGATA CCATATGCTG CTCAGCTTCC 
AGTGGAGAGT CTAAAGCACA TATTAAAAAG AATGACCCGG TGCCAGTAAA ATGCTGTGAG 
ACTGAACTTA TTCCAAAGAG CCAGAAATGC TGTAATGGAG TTGGATATAA TCCTTTGAAA 
TATGTTTGCT CTGACAAGAT TTCAACTGGA ATGATGATGA AGGAAACCAA AGAGTGCAGG 
ATCCTCTGCC CAGCATCTAT GGAAGCCACA GAACATTGTG GCAGGTGTGA CTTCAACTTT 
ACCAGCCACA TTTGCACTGT GATAAGAGGG TCTCACAATT CCACAGGGAA GGCATCAATT 
GAAGAAATGT GTTCATCTGC CGAAGAAACC ATTCATACAG GGAGTGTAAA CACGTACTCT 
TACACAGATG TGAACCTCAA GCCCTACATG ACATATGAGT ACAGGATTTC TGCCTGGAAC 
AGCTATGGGC GAGGACTCAG CAAAGCTGTG AGAGCCAGAA CAAAAGAAGA TGTGCCTCAA 
GGAGTGAGTC CCCCTACGTG GACCAAAATA GACAATCTTG AAGATACAAT TGTCTTAAAC 
TGGAGAAAAC CTATACAATC AAATGGTCCT ATTATTTACT ACATCCTTCT TCGAAATGGA 
ATTGAACGTT TTCGGGGAAC ATCACTGAGC TTCTCTGATA AAGAGGGAAT TCAACCATTT 
CAGGAATATT CATATCAGCT GAAAGCTTGC ACGGTTGCTG GCTGTGCCAC CAGTAGCAAG 
GTAGTTGCAG CTACTACCCA AGGAGTTCCG GAGAGCATCC TGCCACCAAG CATCACAGCC 
CTAAGTGCAG TGGCTCTGCA TCTGAGCTGG AGTGTCCCTG AGAAATCAAA CGGCGTCATT 
AAAGAGTACC AGATCAGGCA GGTTGGGAAA GGTCTCATCC ACACTGACAC CACTGACAGG 
AGACAGCATA CGGTCACAGG TCTCCAGCCA TACACCAACT ACAGCTTCAC TCTTACAGCT 
TGTACATCTG CTGGGTGCAC TTCAAGCGAG CCTTTTCTAG GTCAGACACT GCAGGCAGCT 
CCTGAAGGAG TTTGGGTGAC ACCTCGACAC ATTATCATCA ATTCTACAAC AGTGGAATTA 
TATTGGAGTC TGCCAGAAAA GCCCAATGGC CTCGTTTCTC AATATCAATT GAGTCGTAAT 
GGAAACTTGC TTTTCCTGGG TGGCAGTGAG GAGCAGAATT TCACTGATAA AAACCTGGAG 
CCCAATAGCA GATACACTTA CAAGTTAGAA GTCAAAACTG GAGGTGGCAG CAGTGCTAGT 
GATGATTACA TTGTTCAAAC ACCTATGTCA ACACCAGAAG AAATCTATCC TCCATATAAT 
ATCACAGTAA TTGGGCCTTA TTCTATATTT GTAGCTTGGA TACCACCAGG GATCCTCATC 
CCCGAAATTC CTGTGGAGTA CAATGTCTTA CTCAATGATG GAAGTGTAAC ACCTCTGGCC 
TTCTCCGTTG GTCATCATCA ATCCACCCTT CTGGAAAATT TGACTCCATT CACACAGTAT 
GAGATAAGGA TACAAGCATG TCAAAATGGA AGTTGTGGAG TTAGCAGTAG GATGTTTGTC 
AAAACACCTG AAGCAGCCCC AATGGATCTT AATTCTCCTG TTCTTAAGGC ACTGGGGTCA 
GCTTGCATAG AGATTAAGTG GATGCCACCT GAAAAACCAA ATGGAATCAT CATCAACTAC 
TTTATTTACA GACGCCCTGC TGGCATTGAA GAGGAGTCTG TTTTATTTGT CTGGTCAGAA 
GGAGCCCTTG AATTTATGGA TGAAGGAGAC ACCCTGAGGC CTTTCACACT CTACGAATAT 
CGGGTCAGAG CCTGTAACTC CAAGGGTTCA GTGGAGAGTC TGTGGTCATT AACACAAACT 
CTGGAAGCTC CACCTCAAGA TTTTCCAGCT CCTTGGGCTC AAGCCACGAG TGCTCATTCA 
GTTCTGTTGA ATTGGACAAA GCCAGAATCT CCCAATGGCA TTATCTCCCA TTACCGTGTG 
GTCTACCAGG AGAGACCCGA CGATCCTACA TTTAACAGCC CTACCGTGCA TGCTTTCACA 
GTGAAGGGAA CAAGCCATCA AGCCCACCTG TACGGGTTAG AACCATTCAC AACATATCGC 
ATTGGTGTTG TGGCTGCAAA CCATGCAGGA GAAATTTTAA GCCCTTGGAC TCTGATTCAA 
ACCTTAGAAT CTTCCCCAAG TGGACTGAGA AACTTTATAG TAGAACAGAA AGAGAATGGC 
CGGGCATTGC TACTACAGTG GTCAGAACCT ATGAGAACCA ATGGTGTGAT TAAGACATAC 
AACATCTTCA GTGACGGGTT CCTGGAGTAC TCTGGTTTGA ATCGTCAGTT TCTCTTCCGC 
CGCCTGGATC CTTTCACTCT CTACACACTG ACCCTGGAGG CCTGCACCAG AGCAGGTTGT 
GCACACTCGG CGCCTCAGCC TCTGTGGACA GATGAAGCCC CTCCAGACTC TCAGCTGGCT 
CCTACTGTCC ACTCTGTGAA GTCCACCAGT GTTGAGCTGA GCTGGTCTGA GCCTGTTAAC 
CCAAATGGAA AAATAATTCG CTATGAAGTG ATTCGCAGAT GCTTCGAGGG AAAAGCTTGG 
GGAAATCAGA CGATCCAGGC CGACGAGAAA ATTGTTTTCA CAGAATATAA CACTGAAAGG 
AATACATTTA TGTATAATGA CACAGGTTTG CAACCATGGA CGCAGTGTGA ATATAAAATC 
TACACTTGGA ATTCAGCTGG GCATACCTGT AGCTCTTGGA ATGTGGTGAG GACATTGCAA 
GCACCTCCAG AAGGTCTCTC TCCACCTGTG ATATCCTATG TTTCTATGAA TCCCCAAAAA 
CTGCTGATTT CCTGGATCCC ACCAGAACAG TCTAATGGTA TTATCCAGTC CTATAGGCTT 
CAAAGGAATG AAATGCTCTA TCCTTTTAGC TTTGATCCTG TGACTTTCAA TTACACTGAT 
GAAGAGCTTC TTCCTTTTTC CACCTATAGC TATGCACTCC AAGCCTGCAC GAGTGGAGGA 
TGCTCCACCA GCAAACCCAC CAGCATCACA ACTCTGGAGG CTGCTCCATC AGAAGTCAGC 
CCTCCAGATC TTTGGGCCGT CAGTGCCACT CAAATGAATG TATGTTGGTC ACCGCCCACA 
GTGCAAAATG GAAAGATTAC TAAATATTTA GTTAGATATG ATAATAAAGA GTCCCTTGCT 
GGCCAGGGCC TGTGCCTGCT GGTTTCCCAC CTGCAGCCTT ACTCTCAGTA TAACTTCTCC 
CTTGTAGCCT GCACGAATGG AGGTTGCACA GCTAGTGTGT CAAAATCTGC CTGGACAATG 
GAGGCCCTGC CAGAGAACAT GGACTCTCCA ACATTGCAAG TCACAGGCTC AGAATCAATA 
GAAATCACCT GGAAACCTCC AAGAAACCCA AATGGCCAGA TCAGAAGTTA TGAACTTAGG 
AGGGATGGAA CCATTGTATA TACAGGCTTG GAAACACGCT ATCGTGATTT TACTCTCACC 
CCAGGTGTGG AGTATAGCTA CACAGTAACT GCCAGCAACA GCCAAGGGGG TATTTTGAGT 
CCTCTTGTCA AAGATCGAAC CAGCCCCTCA GCACCCTCAG GGATGGAACC TCCAAAATTG 
CAGGCCAGGG GTCCTCAGGA GATCTTAGTG AACTGGGACC CTCCAGTGAG AACAAATGGT 
GATATCATCA ATTATACCCT CTTCATCCGT GAACTATTTG AAAGAGAAAC TAAAATCATA 
CACATAAACA CAACTCATAA TTCTTTTGGT ATGCAGTCAT ATATAGTAAA CCAGCTGAAG 
CCATTTCACA GGTATGAAAT ACGAATTCAA GCGTGCACCA CCCTGGGATG TGCATCAAGT 
GACTGGACAT TCATACAGAC CCCTGAGATT GCACCTTTGA TGCAACCCCC TCCACATCTG 
GAGGTACAAA TGGCTCCAGG AGGATTCCAG CCAACTGTTT CTCTTTTGTG GACAGGACCG 
CTGCAGCCAA ATGGAAAAGT TTTGTATTAC GAATTATACA GAAGACAAAT AGCAACTCAG 
CCTAGAAAAT CCAATCCAGT CCTAATCTAT AACGGAAGCT CAACATCTTT TATAGATTCC 
GAACTATTGC CTTTCACAGA GTATGAGTAT CAGGTCTGGG CAGTGAATTC TGCAGGAAAA 
GCCCCCAGTA GCTGGACATG GTGCAGAACC GGGCCAGCCC CACCAGAAGG TCTCAGAGCC 
CCCACGTTCC ATGTGATCTC TTCTACCCAA GCAGTGGTCA ACATCAGTGC CCCTGGGAAG 
CCCAACGGGA TCGTCAGTCT CTACAGGCTG TTCTCCAGCA GCGCCCATGG GGCTGAGACA 
GTGCTATCCG AAGGCATGGC CACCCAGCAG ACTCTCCATG GCCTTCAAGC CTTCACTAAC 
TACTCTATTG GAGTAGAGGC CTGCACCTGC TTCAACTGTT GCAGCAAAGG ACCGACAGCT 
GAACTGAGAA CCCATCCTGC CCCACCCTCA GGACTGTCCT CTCCACAAAT CGGGACGCTG 
GCCTCAAGGA CGGCCTCCTT CCGGTGGAGT CCCCCCATGT TCCCCAATGG TGTCATTCAC 
AGCTATGAAC TCCAATTCCA CGTGGCTTGC CCTCCTGACT CAGCCCTCCC CTGTACTCCC 
AGCCAAATAG AAACAAAGTA CACGGGGCTG GGGCAGAAAG CCAGCCTTGG GGGTCTCCAG 
CCCTACACCA CATACAAGCT GAGAGTGGTG GCACACAACG AGGTGGGCAG TACGGCTTCC 
GAGTGGATCA GTTTCACCAC CCAAAAAGAA TTGCCTCAGT ACCGAGCCCC ATTTTCGGTG 
GACAGCAATT TGTCTGTGGT GTGTGTGAAC TGGAGTGACA CCTTCCTCCT GAACGGCCAA 
CTGAAGGAGT ACGTGTTAAC CGACGGAGGG CGACGCGTGT ACAGCGGCTT GGACACCACC 
CTCTACATAC CGAGAACGGC GGACAAAACC TTCTTTTTCC AGGTCATCTG CACGACTGAC 
GAAGGAAGTG TTAAGACGCC GTTGATCCAA TATGATACCT CTACTGGACT TGGCTTGGTC 
CTAACAACTC CTGGGAAAAA GAAGGGATCG CGGAGCAAAA GCACAGAGTT CTACAGCGAG 
CTGTGGTTCA TAGTGTTAAT GGCGATGCTG GGCTTGATCT TGTTGGCCAT TTTTCTGTCC 
CTGATACTAC AAAGAAAAAT CCACAAAGAG CCATATATCA GAGAAAGACC TCCCTTGGTA 
CCTCTTCAGA AGAGGATGTC TCCATTGAAT GTTTACCCAC CGGGGGAAAA CCATATGGGG 
TTAGCCGATA CCAAAATTCC CCGGTCTGGG ACACCTGTGA GTATCCGCAG CAACCGGAGT 
GCATGTGTCC TGCGCATCCC GAGTCAAAAC CAAACCAGCC TAACCTACTC CCAGGGTTCT 
CTTCACCGCA GCGTCAGCCA GCTCATGGAC ATTCAAGACA AGAAAGTCTT GATGGACAAC 
TCACTGTGGG AAGCCATCAT GGGCCACAAC AGTGGACTGT ATGTGGATGA AGAGGACCTG 
ATGAACGCCA TCAAGGATTT CAGCTCAGTG ACTAAGGAAC GCACCACATT CACAGACACC 
CACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996816.2
CDS	388..15996
Translation	MNCPVLSLGSGFLFQVIEMLIFAYFASISLTESRGLFPRLENVGAFKKVSIVPTQAVCGLPDRSTFCHSSAAAESIQFCTQRFCIQDCPYRSSHPTYTALFSAGLSSCITPDKNDLHPNAHSNSASFIFGNHKSCFSSPPSPKLMASFTLAVWLKPEQQGVMCVIEKTVDGQIVFKLTISEKETMFYYRTVNGLQPPIKVMTLGRILVKKWIHLSVQVHQTKISFFINGVEKDHTPFNARTLSGSITDFASGTVQIGQSLNGLEQFVGRMQDFRLYQVALTNREILEVFSGDLLRLHAQSHCRCPGSHPRVHPLAQRYCIPNDAGDTADNRVSRLNPEAHPLSFVNDNDVGTSWVSNVFTNITQLNQGVTISVDLENGQYQVFYIIIQFFSPQPTEIRIQRKKENSLDWEDWQYFARNCGAFGMKNNGDLEKPDSVNCLQLSNFTPYSRGNVTFSILTPGPNYRPGYNNFYNTPSLQEFVKATQIRFHFHGQYYTTETAVNLRHRYYAVDEITISGRCQCHGHADNCDTTSQPYRCLCSQESFTEGLHCDRCLPLYNDKPFRQGDQVYAFNCKPCQCNSHSKSCHYNISVDPFPFEHFRGGGGVCDDCEHNTTGRNCELCKDYFFRQVGADPSAIDVCKPCDCDTVGTRNGSILCDQIGGQCNCKRHVSGRQCNQCQNGFYNLQELDPDGCSPCNCNTSGTVDGDITCHQNSGQCKCKANVIGLRCDHCNFGFKFLRSFNDVGCEPCQCNLHGSVNKFCNPHSGQCECKKEAKGLQCDTCRENFYGLDVTNCKACDCDTAGSLPGTVCNAKTGQCICKPNVEGRQCNKCLEGNFYLRQNNSFLCLPCNCDKTGTINGSLLCNKSTGQCPCKLGVTGLRCNQCEPHRYNLTIDNFQHCQMCECDSLGTLPGTICDPISGQCLCVPNRQGRRCNQCQPGFYISPGNATGCLPCSCHTTGAVNHICNSLTGQCVCQDASIAGQRCDQCKDHYFGFDPQTGRCQPCNCHLSGALNETCHLVTGQCFCKQFVTGSKCDACVPSASHLDVNNLLGCSKTPFQQPPPRGQVQSSSAINLSWSPPDSPNAHWLTYSLLRDGFEIYTTEDQYPYSIQYFLDTDLLPYTKYSYYIETTNVHGSTRSVAVTYKTKPGVPEGNLTLSYIIPIGSDSVTLTWTTLSNQSGPIEKYILSCAPLAGGQPCVSYEGHETSATIWNLVPFAKYDFSVQACTSGGCLHSLPITVTTAQAPPQRLSPPKMQKISSTELHVEWSPPAELNGIIIRYELYMRRLRSTKETTSEESRVFQSSGWLSPHSFVESANENALKPPQTMTTITGLEPYTKYEFRVLAVNMAGSVSSAWVSERTGESAPVFMIPPSVFPLSSYSLNISWEKPADNVTRGKVVGYDINMLSEQSPQQSIPMAFSQLLHTAKSQELSYTVEGLKPYRIYEFTITLCNSVGCVTSASGAGQTLAAAPAQLRPPLVKGINSTTIHLKWFPPEELNGPSPIYQLERRESSLPALMTTMMKGIRFIGNGYCKFPSSTHPVNTDFTGIKASFRTKVPEGLIVFAASPGNQEEYFALQLKKGRLYFLFDPQGSPVEVTTTNDHGKQYSDGKWHEIIAIRHQAFGQITLDGIYTGSSAILNGSTVIGDNTGVFLGGLPRSYTILRKDPEIIQKGFVGCLKDVHFMKNYNPSAIWEPLDWQSSEEQINVYNSWEGCPASLNEGAQFLGAGFLELHPYMFHGGMNFEISFKFRTDQLNGLLLFVYNKDGPDFLAMELKSGILTFRLNTSLAFTQVDLLLGLSYCNGKWNKVIIKKEGSFISASVNGLMKHASESGDQPLVVNSPVYVGGIPQELLNSYQHLCLEQGFGGCMKDVKFTRGAVVNLASVSSGAVRVNLDGCLSTDSAVNCRGNDSILVYQGKEQSVYEGGLQPFTEYLYRVIASHEGGSVYSDWSRGRTTGAAPQSVPTPSRVRSLNGYSIEVTWDEPVVRGVIEKYILKAYSEDSTRPPRMPSASAEFVNTSNLTGILTGLLPFKNYAVTLTACTLAGCTESSHALNISTPQEAPQEVQPPVAKSLPSSLLLSWNPPKKANGIITQYCLYMDGRLIYSGSEENYTVTDLAVFTPHQFLLSACTHVGCTNSSWVLLYTAQLPPEHVDSPVLTVLDSRTIHIQWKQPRKISGILERYVLYMSNHTHDFTIWSVIYNSTELFQDHMLQYVLPGNKYLIKLGACTGGGCTVSEASEALTDEDIPEGVPAPKAHSYSPDSFNVSWTEPEYPNGVITSYGLYLDGILIHNSSELSYRAYGFAPWSLHSFRVQACTAKGCALGPLVENRTLEAPPEGTVNVFVKTQGSRKAHVRWEAPFRPNGLLTHSVLFTGIFYVDPVGNNYTLLNVTKVMYSGEETNLWVLIDGLVPFTNYTVQVNISNSQGSLITDPITIAMPPGAPDGVLPPRLSSATPTSLQVVWSTPARNNAPGSPRYQLQMRSGDSTHGFLELFSNPSASLSYEVSDLQPYTEYMFRLVASNGFGSAHSSWIPFMTAEDKPGPVVPPILLDVKSRMMLVTWQHPRKSNGVITHYNIYLHGRLYLRTPGNVTNCTVMHLHPYTAYKFQVEACTSKGCSLSPESQTVWTLPGAPEGIPSPELFSDTPTSVIISWQPPTHPNGLVENFTIERRVKGKEEVTTLVTLPRSHSMRFIDKTSALSPWTKYEYRVLMSTLHGGTNSSAWVEVTTRPSRPAGVQPPVVTVLEPDAVQVTWKPPLIQNGDILSYEIHMPDPHITLTNVTSAVLSQKVTHLIPFTNYSVTIVACSGGNGYLGGCTESLPTYVTTHPTVPQNVGPLSVIPLSESYVVISWQPPSKPNGPNLRYELLRRKIQQPLASNPPEDLNRWHNIYSGTQWLYEDKGLSRFTTYEYMLFVHNSVGFTPSREVTVTTLAGLPERGANLTASVLNHTAIDVRWAKPTVQDLQGEVEYYTLFWSSATSNDSLKILPDVNSHVIGHLKPNTEYWIFISVFNGVHSINSAGLHATTCDGEPQGMLPPEVVIINSTAVRVIWTSPSNPNGVVTEYSIYVNNKLYKTGMNVPGSFILRDLSPFTIYDIQVEVCTIYACVKSNGTQITTVEDTPSDIPTPTIRGITSRSLQIDWVSPRKPNGIILGYDLLWKTWYPCAKTQKLVQDQSDELCKAVRCQKPESICGHICYSSEAKVCCNGVLYNPKPGHRCCEEKYIPFVLNSTGVCCGGRIQEAQPNHQCCSGYYARILPGEVCCPDEQHNRVSVGIGDSCCGRMPYSTSGNQICCAGRLHDGHGQKCCGRQIVSNDLECCGGEEGVVYNRLPGMFCCGQDYVNMSDTICCSASSGESKAHIKKNDPVPVKCCETELIPKSQKCCNGVGYNPLKYVCSDKISTGMMMKETKECRILCPASMEATEHCGRCDFNFTSHICTVIRGSHNSTGKASIEEMCSSAEETIHTGSVNTYSYTDVNLKPYMTYEYRISAWNSYGRGLSKAVRARTKEDVPQGVSPPTWTKIDNLEDTIVLNWRKPIQSNGPIIYYILLRNGIERFRGTSLSFSDKEGIQPFQEYSYQLKACTVAGCATSSKVVAATTQGVPESILPPSITALSAVALHLSWSVPEKSNGVIKEYQIRQVGKGLIHTDTTDRRQHTVTGLQPYTNYSFTLTACTSAGCTSSEPFLGQTLQAAPEGVWVTPRHIIINSTTVELYWSLPEKPNGLVSQYQLSRNGNLLFLGGSEEQNFTDKNLEPNSRYTYKLEVKTGGGSSASDDYIVQTPMSTPEEIYPPYNITVIGPYSIFVAWIPPGILIPEIPVEYNVLLNDGSVTPLAFSVGHHQSTLLENLTPFTQYEIRIQACQNGSCGVSSRMFVKTPEAAPMDLNSPVLKALGSACIEIKWMPPEKPNGIIINYFIYRRPAGIEEESVLFVWSEGALEFMDEGDTLRPFTLYEYRVRACNSKGSVESLWSLTQTLEAPPQDFPAPWAQATSAHSVLLNWTKPESPNGIISHYRVVYQERPDDPTFNSPTVHAFTVKGTSHQAHLYGLEPFTTYRIGVVAANHAGEILSPWTLIQTLESSPSGLRNFIVEQKENGRALLLQWSEPMRTNGVIKTYNIFSDGFLEYSGLNRQFLFRRLDPFTLYTLTLEACTRAGCAHSAPQPLWTDEAPPDSQLAPTVHSVKSTSVELSWSEPVNPNGKIIRYEVIRRCFEGKAWGNQTIQADEKIVFTEYNTERNTFMYNDTGLQPWTQCEYKIYTWNSAGHTCSSWNVVRTLQAPPEGLSPPVISYVSMNPQKLLISWIPPEQSNGIIQSYRLQRNEMLYPFSFDPVTFNYTDEELLPFSTYSYALQACTSGGCSTSKPTSITTLEAAPSEVSPPDLWAVSATQMNVCWSPPTVQNGKITKYLVRYDNKESLAGQGLCLLVSHLQPYSQYNFSLVACTNGGCTASVSKSAWTMEALPENMDSPTLQVTGSESIEITWKPPRNPNGQIRSYELRRDGTIVYTGLETRYRDFTLTPGVEYSYTVTASNSQGGILSPLVKDRTSPSAPSGMEPPKLQARGPQEILVNWDPPVRTNGDIINYTLFIRELFERETKIIHINTTHNSFGMQSYIVNQLKPFHRYEIRIQACTTLGCASSDWTFIQTPEIAPLMQPPPHLEVQMAPGGFQPTVSLLWTGPLQPNGKVLYYELYRRQIATQPRKSNPVLIYNGSSTSFIDSELLPFTEYEYQVWAVNSAGKAPSSWTWCRTGPAPPEGLRAPTFHVISSTQAVVNISAPGKPNGIVSLYRLFSSSAHGAETVLSEGMATQQTLHGLQAFTNYSIGVEACTCFNCCSKGPTAELRTHPAPPSGLSSPQIGTLASRTASFRWSPPMFPNGVIHSYELQFHVACPPDSALPCTPSQIETKYTGLGQKASLGGLQPYTTYKLRVVAHNEVGSTASEWISFTTQKELPQYRAPFSVDSNLSVVCVNWSDTFLLNGQLKEYVLTDGGRRVYSGLDTTLYIPRTADKTFFFQVICTTDEGSVKTPLIQYDTSTGLGLVLTTPGKKKGSRSKSTEFYSELWFIVLMAMLGLILLAIFLSLILQRKIHKEPYIRERPPLVPLQKRMSPLNVYPPGENHMGLADTKIPRSGTPVSIRSNRSACVLRIPSQNQTSLTYSQGSLHRSVSQLMDIQDKKVLMDNSLWEAIMGHNSGLYVDEEDLMNAIKDFSSVTKERTTFTDTHL

Target ORF information:

RefSeq Version	NM_206933.2
Organism	Homo sapiens(human)
Definition	Homo sapiens usherin (USH2A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206933.2

ORF Insert Sequence:

ATGAATTGCC CAGTTCTTTC ATTGGGCTCT GGCTTCTTGT TTCAGGTCAT TGAAATGTTG 
ATCTTTGCCT ATTTTGCTTC AATATCCTTG ACTGAGTCAC GAGGTCTTTT CCCAAGGCTG 
GAGAACGTGG GAGCTTTCAA GAAAGTTTCC ATCGTGCCAA CCCAAGCAGT ATGTGGACTC 
CCAGACCGAA GCACTTTTTG TCACAGCTCT GCTGCTGCTG AAAGTATTCA GTTCTGTACC 
CAGCGGTTTT GTATTCAGGA TTGCCCATAC AGATCTTCAC ACCCTACCTA CACTGCCCTT 
TTCTCAGCAG GCCTCAGTAG CTGCATCACA CCAGACAAGA ATGATCTGCA TCCTAACGCC 
CATAGCAATT CTGCAAGTTT TATTTTTGGA AATCACAAGA GCTGCTTTTC TTCTCCTCCT 
TCTCCAAAGC TGATGGCATC ATTTACCTTA GCTGTATGGC TGAAACCTGA GCAACAAGGT 
GTAATGTGTG TTATAGAAAA GACAGTAGAT GGGCAGATTG TGTTCAAACT TACAATATCT 
GAGAAAGAGA CCATGTTTTA TTATCGCACA GTAAATGGTT TGCAACCTCC AATAAAAGTA 
ATGACACTGG GGAGAATTCT TGTGAAGAAA TGGATTCATC TTAGTGTGCA GGTGCATCAG 
ACAAAAATCA GCTTCTTTAT CAATGGCGTG GAGAAGGATC ATACACCTTT CAATGCAAGA 
ACTCTAAGTG GTTCAATTAC AGATTTTGCA TCTGGTACTG TGCAAATAGG ACAGAGTTTA 
AATGGTTTAG AGCAGTTTGT CGGAAGAATG CAAGATTTTC GATTATACCA AGTGGCACTT 
ACAAACAGAG AGATTCTGGA AGTCTTCTCT GGAGATCTTC TCAGATTGCA TGCCCAATCA 
CATTGCCGTT GCCCTGGCAG CCACCCGCGG GTCCACCCTT TGGCACAGCG GTACTGCATT 
CCTAATGATG CAGGAGACAC AGCTGATAAT AGAGTGTCAC GGTTGAATCC TGAAGCCCAT 
CCTCTCTCTT TTGTCAATGA TAATGATGTT GGTACTTCAT GGGTTTCAAA TGTGTTTACA 
AACATTACAC AGCTTAATCA AGGAGTGACT ATTTCAGTTG ATTTGGAAAA TGGACAGTAT 
CAGGTGTTTT ATATTATCAT TCAGTTCTTT AGTCCACAAC CAACGGAAAT AAGGATTCAA 
AGGAAGAAGG AAAATAGTTT AGATTGGGAG GACTGGCAAT ATTTTGCCAG GAATTGTGGT 
GCTTTTGGAA TGAAAAACAA TGGAGATTTG GAAAAACCTG ATTCTGTCAA CTGTCTTCAG 
CTTTCCAATT TTACTCCATA TTCCCGTGGC AATGTCACAT TTAGCATCCT GACACCTGGA 
CCAAATTATC GTCCTGGATA CAATAACTTC TATAATACCC CATCTCTTCA AGAGTTCGTA 
AAAGCCACGC AAATAAGGTT TCATTTTCAT GGGCAGTACT ATACAACTGA GACTGCTGTT 
AACCTCAGAC ACAGATATTA TGCAGTGGAC GAAATCACCA TTAGTGGGAG ATGTCAGTGC 
CATGGTCATG CCGATAACTG CGACACAACA AGCCAGCCAT ATAGATGCCT CTGCTCCCAG 
GAGAGCTTCA CTGAAGGACT TCATTGTGAT CGCTGCTTGC CTCTTTATAA TGACAAGCCT 
TTCCGCCAAG GTGATCAAGT TTACGCTTTC AATTGTAAAC CTTGTCAATG CAACAGCCAT 
TCCAAAAGCT GCCATTACAA CATCTCTGTA GACCCATTTC CTTTTGAGCA CTTCAGAGGG 
GGAGGAGGAG TTTGTGATGA TTGTGAGCAT AACACTACAG GAAGGAACTG TGAGCTGTGC 
AAGGATTACT TTTTCCGACA AGTTGGTGCA GATCCTTCGG CCATAGATGT TTGCAAACCC 
TGTGACTGTG ATACAGTTGG CACTAGAAAT GGTAGCATTC TTTGTGATCA GATTGGAGGA 
CAGTGTAATT GTAAGAGACA CGTGTCTGGC AGGCAGTGCA ATCAGTGCCA GAATGGATTC 
TACAATCTAC AAGAGTTGGA TCCTGATGGC TGCAGTCCCT GTAACTGCAA TACCTCTGGG 
ACAGTGGATG GAGATATTAC CTGTCACCAA AATTCAGGCC AGTGCAAGTG CAAAGCAAAC 
GTTATTGGGC TTAGGTGTGA TCATTGCAAT TTTGGATTTA AATTTCTCCG AAGCTTTAAT 
GATGTTGGAT GTGAGCCCTG CCAGTGTAAC CTCCATGGCT CAGTGAACAA ATTCTGCAAT 
CCTCACTCTG GGCAGTGTGA GTGCAAAAAA GAAGCCAAAG GACTTCAGTG TGACACCTGC 
AGAGAAAACT TTTATGGGTT AGATGTCACC AATTGTAAGG CCTGTGACTG TGACACAGCT 
GGATCCCTCC CTGGGACTGT CTGTAATGCT AAGACAGGGC AGTGCATCTG CAAGCCCAAT 
GTTGAAGGGA GACAGTGCAA TAAATGTTTG GAGGGAAACT TCTACCTACG GCAAAATAAT 
TCTTTCCTCT GTCTGCCTTG CAACTGTGAT AAGACTGGGA CAATAAATGG CTCTCTGCTG 
TGTAACAAAT CAACAGGACA ATGTCCTTGC AAATTAGGGG TAACAGGTCT TCGCTGTAAT 
CAGTGTGAGC CTCACAGGTA CAATTTGACC ATTGACAATT TTCAACACTG CCAGATGTGT 
GAGTGTGATT CCTTGGGGAC ATTACCTGGG ACCATTTGTG ACCCAATCAG TGGCCAGTGC 
CTGTGTGTGC CTAATCGTCA AGGAAGAAGG TGTAATCAGT GTCAACCAGG TTTTTATATT 
TCTCCAGGCA ATGCCACTGG CTGCCTGCCA TGCTCATGCC ATACAACTGG TGCAGTTAAT 
CACATCTGTA ATAGCCTGAC TGGTCAGTGT GTTTGCCAAG ATGCTTCCAT TGCTGGGCAA 
CGTTGTGACC AATGCAAAGA CCATTACTTT GGATTTGATC CTCAGACTGG AAGATGTCAG 
CCTTGTAATT GTCATCTCTC AGGAGCCTTG AATGAAACCT GTCACTTGGT CACAGGCCAG 
TGTTTCTGTA AACAATTTGT CACTGGCTCA AAGTGTGATG CTTGTGTTCC CAGTGCAAGC 
CACTTGGATG TCAACAATCT ATTGGGTTGC AGCAAAACTC CATTCCAGCA ACCTCCGCCC 
AGAGGACAAG TTCAAAGTTC TTCTGCTATC AATCTCTCCT GGAGTCCACC TGATTCTCCA 
AATGCCCACT GGCTTACTTA CAGTTTACTC AGGGATGGTT TTGAAATCTA CACAACAGAG 
GATCAATACC CATACAGTAT TCAATACTTC TTAGACACAG ACCTGTTACC ATATACCAAA 
TATTCCTATT ACATTGAGAC CACCAATGTG CATGGTTCAA CAAGGAGTGT AGCTGTCACT 
TACAAGACAA AACCAGGGGT CCCAGAGGGA AACTTGACTT TAAGTTATAT CATTCCTATT 
GGCTCAGACT CTGTGACACT TACCTGGACA ACACTCTCAA ATCAATCTGG TCCCATAGAG 
AAATATATTT TGTCCTGTGC CCCTTTGGCT GGTGGTCAGC CATGTGTTTC CTACGAAGGT 
CATGAAACCT CAGCTACCAT CTGGAATCTG GTTCCATTTG CCAAGTACGA TTTTTCTGTA 
CAGGCGTGTA CTAGCGGGGG CTGTTTACAC AGCTTGCCCA TTACAGTGAC CACAGCCCAG 
GCCCCTCCCC AAAGACTAAG TCCACCTAAG ATGCAGAAAA TCAGTTCTAC AGAACTTCAT 
GTAGAATGGT CTCCACCAGC GGAACTAAAT GGAATAATTA TAAGATATGA ACTATACATG 
AGAAGACTGA GATCTACTAA AGAAACCACA TCTGAGGAAA GTCGAGTTTT TCAGAGCAGT 
GGTTGGCTCA GTCCTCATTC ATTTGTAGAA TCGGCCAATG AAAATGCATT AAAACCTCCT 
CAAACAATGA CAACCATCAC TGGCTTGGAG CCATACACCA AGTATGAGTT CAGAGTCTTA 
GCTGTGAATA TGGCTGGAAG TGTGTCTTCT GCCTGGGTCT CAGAAAGAAC GGGAGAATCA 
GCACCTGTAT TCATGATCCC TCCTTCAGTC TTTCCCCTCT CTTCGTACTC TCTCAATATC 
TCCTGGGAGA AGCCAGCAGA TAATGTTACA AGAGGAAAAG TTGTGGGGTA TGACATCAAT 
ATGCTTTCTG AACAATCACC TCAACAGTCT ATTCCCATGG CGTTTTCACA GCTGTTGCAC 
ACTGCTAAAT CCCAAGAACT ATCTTACACT GTAGAAGGAC TGAAACCTTA TAGGATATAT 
GAGTTTACTA TTACTCTCTG CAATTCAGTT GGTTGTGTGA CCAGTGCTTC GGGAGCAGGA 
CAAACTTTAG CAGCAGCACC AGCACAACTG AGGCCACCTC TGGTTAAAGG AATCAACAGC 
ACAACAATCC ATCTTAAGTG GTTTCCACCT GAAGAACTGA ATGGACCCTC TCCTATATAT 
CAGCTGGAAA GGAGAGAGTC ATCTCTACCA GCTCTGATGA CCACGATGAT GAAAGGAATC 
CGTTTCATAG GAAATGGGTA TTGTAAATTT CCCAGCTCCA CTCACCCAGT CAATACAGAC 
TTCACTGGCA TTAAGGCCAG CTTTCGAACA AAAGTGCCTG AAGGTTTGAT TGTCTTTGCA 
GCATCACCTG GCAATCAGGA AGAGTATTTT GCACTTCAGT TGAAGAAGGG ACGTCTTTAT 
TTTCTTTTTG ATCCTCAGGG GTCACCAGTG GAAGTAACTA CAACTAATGA TCATGGCAAA 
CAATATAGTG ATGGAAAATG GCATGAAATA ATTGCTATTA GGCATCAGGC TTTTGGCCAA 
ATCACTCTGG ATGGGATATA TACAGGTTCC TCTGCCATCC TGAATGGTAG TACTGTTATT 
GGAGATAACA CAGGAGTCTT TCTGGGAGGG CTCCCGCGAA GTTATACCAT CCTCAGGAAG 
GATCCTGAGA TAATCCAAAA AGGTTTTGTG GGCTGTCTCA AGGATGTACA TTTTATGAAG 
AATTACAATC CGTCAGCTAT TTGGGAACCT CTGGATTGGC AGAGTTCTGA AGAACAAATC 
AACGTGTATA ACAGCTGGGA GGGATGTCCC GCTTCATTAA ATGAGGGAGC TCAGTTCCTA 
GGAGCAGGGT TCCTGGAACT TCATCCATAT ATGTTTCATG GTGGAATGAA CTTTGAGATT 
TCCTTTAAGT TCAGAACTGA CCAATTAAAT GGATTGCTTC TTTTCGTTTA TAACAAAGAT 
GGACCTGATT TTCTTGCTAT GGAGCTGAAA AGTGGAATAT TGACCTTCCG GTTAAATACC 
AGTCTTGCCT TTACACAAGT GGATCTATTG CTGGGGCTAT CCTATTGTAA TGGAAAGTGG 
AATAAAGTCA TTATTAAAAA GGAAGGCTCT TTCATATCAG CAAGTGTGAA TGGACTGATG 
AAGCATGCAT CGGAGTCCGG AGACCAGCCA CTGGTGGTGA ATTCACCAGT TTATGTGGGA 
GGAATCCCAC AGGAACTGCT GAACTCTTAT CAACATTTGT GTTTGGAACA AGGTTTCGGT 
GGTTGCATGA AGGATGTTAA ATTTACACGG GGTGCTGTCG TTAACTTGGC ATCTGTGTCC 
AGCGGTGCTG TCAGAGTCAA TCTGGATGGA TGCCTATCAA CTGACAGTGC TGTTAACTGC 
AGGGGAAATG ACTCCATCCT GGTTTACCAG GGAAAAGAGC AGAGTGTTTA CGAGGGTGGT 
CTCCAGCCTT TTACAGAATA CCTGTATCGA GTGATAGCCT CGCATGAAGG AGGTTCAGTA 
TATAGTGATT GGAGTCGAGG ACGTACAACA GGAGCAGCTC CACAAAGTGT GCCAACTCCC 
TCAAGAGTCC GCAGCTTAAA TGGATACAGC ATTGAGGTGA CCTGGGATGA ACCTGTTGTC 
AGAGGTGTAA TTGAGAAGTA CATTCTGAAA GCCTATAGTG AGGACAGCAC CCGTCCACCC 
CGCATGCCCT CTGCCAGTGC TGAATTTGTC AATACAAGCA ACCTCACAGG CATATTGACA 
GGCTTGCTAC CCTTCAAAAA CTATGCAGTA ACCCTAACTG CTTGCACTTT GGCTGGCTGT 
ACTGAGAGCT CACATGCATT GAACATCTCT ACTCCACAAG AAGCCCCACA AGAGGTTCAG 
CCACCAGTAG CCAAATCCCT TCCCAGTTCT TTGCTGCTCT CCTGGAACCC ACCCAAAAAG 
GCAAATGGTA TTATAACTCA GTACTGTTTA TACATGGATG GGAGGCTGAT CTATTCAGGC 
AGTGAGGAGA ACTACACAGT CACAGATTTA GCAGTATTTA CACCCCACCA GTTTCTACTA 
AGTGCATGCA CACATGTGGG CTGTACAAAC AGTTCCTGGG TCCTACTGTA CACAGCACAG 
CTGCCACCAG AACACGTGGA TTCCCCAGTT CTGACTGTCC TGGATTCTAG AACTATACAC 
ATACAGTGGA AACAACCAAG AAAAATAAGT GGGATTCTGG AACGCTATGT ATTATATATG 
TCAAACCATA CACATGATTT TACAATTTGG AGTGTCATCT ATAACAGTAC AGAACTTTTC 
CAGGATCATA TGCTACAATA CGTTTTACCT GGTAATAAAT ATCTCATCAA GCTGGGAGCT 
TGCACAGGTG GTGGGTGCAC AGTGAGTGAG GCCAGTGAGG CCCTAACTGA CGAGGACATA 
CCCGAAGGCG TGCCAGCCCC CAAAGCCCAC TCATATTCAC CTGACTCCTT TAATGTCTCC 
TGGACTGAGC CTGAATATCC GAATGGTGTT ATCACGAGTT ATGGATTATA TCTAGATGGT 
ATATTAATCC ACAATTCCTC AGAACTCAGC TATCGTGCTT ACGGATTTGC TCCTTGGAGT 
TTACATTCCT TCAGAGTCCA AGCATGCACG GCCAAAGGTT GTGCTCTGGG CCCACTGGTG 
GAAAATCGAA CTCTAGAAGC TCCTCCTGAA GGAACAGTAA ATGTGTTTGT CAAAACACAG 
GGATCCCGGA AAGCCCACGT GAGGTGGGAA GCACCTTTTC GCCCTAATGG ACTCTTAACA 
CACTCAGTCC TTTTCACTGG GATATTCTAT GTAGACCCAG TAGGTAATAA CTACACCCTT 
CTGAATGTCA CAAAAGTCAT GTACAGCGGA GAAGAGACAA ACCTTTGGGT GCTCATCGAT 
GGGCTGGTTC CTTTTACCAA CTATACTGTA CAAGTGAATA TTTCAAATAG CCAAGGCAGC 
TTGATAACTG ATCCTATAAC AATTGCAATG CCTCCAGGAG CTCCAGATGG CGTGCTGCCT 
CCCAGGCTTT CATCTGCCAC TCCAACCAGT CTTCAGGTTG TCTGGTCTAC ACCAGCTCGT 
AATAACGCTC CTGGCTCTCC CAGATACCAA CTCCAGATGA GGTCTGGCGA CTCCACCCAT 
GGATTTCTAG AGTTATTTTC CAATCCTTCT GCATCGTTAA GCTATGAAGT GAGTGATCTC 
CAACCGTACA CAGAGTATAT GTTTCGGTTG GTTGCCTCCA ATGGATTTGG CAGTGCACAT 
AGTTCTTGGA TTCCATTCAT GACCGCAGAG GACAAACCTG GACCTGTAGT TCCTCCGATT 
CTTCTGGATG TGAAGTCAAG AATGATGTTG GTCACCTGGC AGCATCCTAG AAAATCCAAT 
GGGGTTATTA CCCATTATAA CATTTATCTA CATGGCCGTC TATACTTGAG AACTCCTGGA 
AATGTCACTA ATTGCACAGT GATGCATTTA CACCCATACA CTGCCTATAA GTTTCAGGTA 
GAAGCCTGCA CTTCAAAAGG ATGTTCCCTT TCACCAGAGT CCCAGACTGT ATGGACACTC 
CCAGGGGCAC CGGAAGGGAT CCCAAGTCCA GAGCTGTTCT CTGATACTCC AACATCTGTG 
ATTATATCTT GGCAACCCCC TACCCACCCC AATGGCTTGG TGGAGAATTT CACAATTGAG 
AGAAGAGTCA AAGGAAAGGA AGAAGTTACT ACCCTGGTGA CTCTCCCGAG GAGTCATTCC 
ATGAGGTTTA TTGACAAGAC TTCTGCTCTT AGCCCATGGA CAAAATATGA ATATCGGGTA 
CTGATGAGCA CTCTTCATGG AGGCACAAAC AGCAGTGCTT GGGTAGAAGT TACCACAAGA 
CCCTCACGAC CTGCTGGGGT GCAGCCACCT GTGGTGACAG TGCTGGAACC CGATGCAGTC 
CAGGTCACTT GGAAACCCCC ACTCATCCAG AACGGAGACA TACTTAGCTA TGAGATTCAC 
ATGCCTGACC CTCACATCAC TTTAACCAAT GTGACTTCCG CAGTGTTAAG TCAAAAAGTT 
ACTCATCTGA TTCCTTTCAC TAATTATTCT GTCACCATTG TTGCTTGCTC AGGGGGTAAT 
GGGTACCTTG GAGGGTGCAC AGAGAGTTTA CCTACCTATG TTACCACTCA CCCCACCGTA 
CCTCAGAATG TTGGCCCATT GTCTGTGATT CCACTAAGTG AATCATATGT TGTGATTTCT 
TGGCAACCAC CATCCAAGCC AAATGGACCT AATTTGAGAT ATGAGCTTCT GAGACGTAAA 
ATCCAGCAGC CACTTGCATC AAATCCCCCA GAAGATTTAA ATCGGTGGCA CAATATTTAT 
TCAGGAACTC AGTGGCTTTA TGAAGATAAG GGTCTTAGCA GGTTTACAAC CTATGAATAT 
ATGCTCTTCG TACACAACAG TGTGGGTTTT ACACCGAGCC GAGAAGTGAC TGTGACAACG 
TTAGCTGGTC TTCCAGAGAG AGGAGCCAAT CTCACTGCGA GTGTCCTTAA CCACACAGCC 
ATCGACGTGA GGTGGGCTAA ACCAACTGTT CAAGACCTAC AAGGTGAAGT TGAATATTAC 
ACACTTTTTT GGAGTTCTGC TACCTCAAAC GACTCTCTAA AAATCTTGCC AGATGTAAAC 
TCTCATGTCA TTGGCCACCT AAAGCCAAAC ACAGAGTATT GGATCTTTAT CTCTGTCTTC 
AATGGAGTCC ACAGCATCAA CAGTGCAGGA CTTCATGCAA CCACTTGCGA TGGGGAGCCT 
CAGGGCATGC TTCCTCCAGA GGTTGTCATC ATCAACAGTA CAGCTGTACG TGTCATCTGG 
ACATCTCCTT CAAACCCAAA TGGTGTTGTC ACTGAGTATT CTATCTATGT AAATAATAAG 
CTCTACAAGA CTGGAATGAA TGTGCCTGGG TCGTTTATTC TGAGAGACCT GTCTCCCTTC 
ACTATCTATG ACATTCAGGT TGAAGTCTGC ACAATATATG CCTGCGTGAA AAGCAATGGA 
ACCCAAATTA CCACTGTGGA AGACACTCCA AGTGATATAC CAACACCCAC AATTCGTGGC 
ATCACTTCAA GATCTCTTCA AATTGATTGG GTGTCTCCAC GGAAGCCAAA TGGCATCATT 
CTTGGATATG ATCTCCTATG GAAAACATGG TATCCATGCG CTAAAACTCA AAAGTTAGTG 
CAGGATCAGA GTGATGAGCT CTGCAAGGCA GTGAGGTGTC AAAAACCTGA ATCTATCTGT 
GGACACATTT GCTATTCTTC TGAAGCTAAG GTTTGTTGTA ACGGAGTGCT CTATAACCCC 
AAGCCTGGAC ATCGCTGTTG TGAAGAAAAG TATATCCCGT TTGTTCTGAA TTCTACTGGA 
GTTTGTTGTG GTGGCCGAAT ACAGGAGGCA CAACCAAATC ATCAGTGCTG CTCTGGGTAT 
TACGCTAGAA TTCTACCAGG TGAAGTATGC TGTCCAGATG AACAGCACAA TCGGGTTTCT 
GTTGGCATTG GTGATTCCTG CTGTGGCAGA ATGCCGTACT CCACCTCAGG AAACCAGATT 
TGCTGTGCTG GGAGGCTTCA TGATGGCCAT GGCCAGAAGT GCTGTGGCAG ACAGATTGTG 
AGCAACGATT TAGAGTGTTG TGGTGGAGAA GAAGGAGTGG TGTACAATCG CCTTCCAGGT 
ATGTTCTGTT GTGGGCAGGA TTATGTGAAT ATGTCAGATA CCATATGCTG CTCAGCTTCC 
AGTGGAGAGT CTAAAGCACA TATTAAAAAG AATGACCCGG TGCCAGTAAA ATGCTGTGAG 
ACTGAACTTA TTCCAAAGAG CCAGAAATGC TGTAATGGAG TTGGATATAA TCCTTTGAAA 
TATGTTTGCT CTGACAAGAT TTCAACTGGA ATGATGATGA AGGAAACCAA AGAGTGCAGG 
ATCCTCTGCC CAGCATCTAT GGAAGCCACA GAACATTGTG GCAGGTGTGA CTTCAACTTT 
ACCAGCCACA TTTGCACTGT GATAAGAGGG TCTCACAATT CCACAGGGAA GGCATCAATT 
GAAGAAATGT GTTCATCTGC CGAAGAAACC ATTCATACAG GGAGTGTAAA CACGTACTCT 
TACACAGATG TGAACCTCAA GCCCTACATG ACATATGAGT ACAGGATTTC TGCCTGGAAC 
AGCTATGGGC GAGGACTCAG CAAAGCTGTG AGAGCCAGAA CAAAAGAAGA TGTGCCTCAA 
GGAGTGAGTC CCCCTACGTG GACCAAAATA GACAATCTTG AAGATACAAT TGTCTTAAAC 
TGGAGAAAAC CTATACAATC AAATGGTCCT ATTATTTACT ACATCCTTCT TCGAAATGGA 
ATTGAACGTT TTCGGGGAAC ATCACTGAGC TTCTCTGATA AAGAGGGAAT TCAACCATTT 
CAGGAATATT CATATCAGCT GAAAGCTTGC ACGGTTGCTG GCTGTGCCAC CAGTAGCAAG 
GTAGTTGCAG CTACTACCCA AGGAGTTCCG GAGAGCATCC TGCCACCAAG CATCACAGCC 
CTAAGTGCAG TGGCTCTGCA TCTGAGCTGG AGTGTCCCTG AGAAATCAAA CGGCGTCATT 
AAAGAGTACC AGATCAGGCA GGTTGGGAAA GGTCTCATCC ACACTGACAC CACTGACAGG 
AGACAGCATA CGGTCACAGG TCTCCAGCCA TACACCAACT ACAGCTTCAC TCTTACAGCT 
TGTACATCTG CTGGGTGCAC TTCAAGCGAG CCTTTTCTAG GTCAGACACT GCAGGCAGCT 
CCTGAAGGAG TTTGGGTGAC ACCTCGACAC ATTATCATCA ATTCTACAAC AGTGGAATTA 
TATTGGAGTC TGCCAGAAAA GCCCAATGGC CTCGTTTCTC AATATCAATT GAGTCGTAAT 
GGAAACTTGC TTTTCCTGGG TGGCAGTGAG GAGCAGAATT TCACTGATAA AAACCTGGAG 
CCCAATAGCA GATACACTTA CAAGTTAGAA GTCAAAACTG GAGGTGGCAG CAGTGCTAGT 
GATGATTACA TTGTTCAAAC ACCTATGTCA ACACCAGAAG AAATCTATCC TCCATATAAT 
ATCACAGTAA TTGGGCCTTA TTCTATATTT GTAGCTTGGA TACCACCAGG GATCCTCATC 
CCCGAAATTC CTGTGGAGTA CAATGTCTTA CTCAATGATG GAAGTGTAAC ACCTCTGGCC 
TTCTCCGTTG GTCATCATCA ATCCACCCTT CTGGAAAATT TGACTCCATT CACACAGTAT 
GAGATAAGGA TACAAGCATG TCAAAATGGA AGTTGTGGAG TTAGCAGTAG GATGTTTGTC 
AAAACACCTG AAGCAGCCCC AATGGATCTT AATTCTCCTG TTCTTAAGGC ACTGGGGTCA 
GCTTGCATAG AGATTAAGTG GATGCCACCT GAAAAACCAA ATGGAATCAT CATCAACTAC 
TTTATTTACA GACGCCCTGC TGGCATTGAA GAGGAGTCTG TTTTATTTGT CTGGTCAGAA 
GGAGCCCTTG AATTTATGGA TGAAGGAGAC ACCCTGAGGC CTTTCACACT CTACGAATAT 
CGGGTCAGAG CCTGTAACTC CAAGGGTTCA GTGGAGAGTC TGTGGTCATT AACACAAACT 
CTGGAAGCTC CACCTCAAGA TTTTCCAGCT CCTTGGGCTC AAGCCACGAG TGCTCATTCA 
GTTCTGTTGA ATTGGACAAA GCCAGAATCT CCCAATGGCA TTATCTCCCA TTACCGTGTG 
GTCTACCAGG AGAGACCCGA CGATCCTACA TTTAACAGCC CTACCGTGCA TGCTTTCACA 
GTGAAGGGAA CAAGCCATCA AGCCCACCTG TACGGGTTAG AACCATTCAC AACATATCGC 
ATTGGTGTTG TGGCTGCAAA CCATGCAGGA GAAATTTTAA GCCCTTGGAC TCTGATTCAA 
ACCTTAGAAT CTTCCCCAAG TGGACTGAGA AACTTTATAG TAGAACAGAA AGAGAATGGC 
CGGGCATTGC TACTACAGTG GTCAGAACCT ATGAGAACCA ATGGTGTGAT TAAGACATAC 
AACATCTTCA GTGACGGGTT CCTGGAGTAC TCTGGTTTGA ATCGTCAGTT TCTCTTCCGC 
CGCCTGGATC CTTTCACTCT CTACACACTG ACCCTGGAGG CCTGCACCAG AGCAGGTTGT 
GCACACTCGG CGCCTCAGCC TCTGTGGACA GATGAAGCCC CTCCAGACTC TCAGCTGGCT 
CCTACTGTCC ACTCTGTGAA GTCCACCAGT GTTGAGCTGA GCTGGTCTGA GCCTGTTAAC 
CCAAATGGAA AAATAATTCG CTATGAAGTG ATTCGCAGAT GCTTCGAGGG AAAAGCTTGG 
GGAAATCAGA CGATCCAGGC CGACGAGAAA ATTGTTTTCA CAGAATATAA CACTGAAAGG 
AATACATTTA TGTATAATGA CACAGGTTTG CAACCATGGA CGCAGTGTGA ATATAAAATC 
TACACTTGGA ATTCAGCTGG GCATACCTGT AGCTCTTGGA ATGTGGTGAG GACATTGCAA 
GCACCTCCAG AAGGTCTCTC TCCACCTGTG ATATCCTATG TTTCTATGAA TCCCCAAAAA 
CTGCTGATTT CCTGGATCCC ACCAGAACAG TCTAATGGTA TTATCCAGTC CTATAGGCTT 
CAAAGGAATG AAATGCTCTA TCCTTTTAGC TTTGATCCTG TGACTTTCAA TTACACTGAT 
GAAGAGCTTC TTCCTTTTTC CACCTATAGC TATGCACTCC AAGCCTGCAC GAGTGGAGGA 
TGCTCCACCA GCAAACCCAC CAGCATCACA ACTCTGGAGG CTGCTCCATC AGAAGTCAGC 
CCTCCAGATC TTTGGGCCGT CAGTGCCACT CAAATGAATG TATGTTGGTC ACCGCCCACA 
GTGCAAAATG GAAAGATTAC TAAATATTTA GTTAGATATG ATAATAAAGA GTCCCTTGCT 
GGCCAGGGCC TGTGCCTGCT GGTTTCCCAC CTGCAGCCTT ACTCTCAGTA TAACTTCTCC 
CTTGTAGCCT GCACGAATGG AGGTTGCACA GCTAGTGTGT CAAAATCTGC CTGGACAATG 
GAGGCCCTGC CAGAGAACAT GGACTCTCCA ACATTGCAAG TCACAGGCTC AGAATCAATA 
GAAATCACCT GGAAACCTCC AAGAAACCCA AATGGCCAGA TCAGAAGTTA TGAACTTAGG 
AGGGATGGAA CCATTGTATA TACAGGCTTG GAAACACGCT ATCGTGATTT TACTCTCACC 
CCAGGTGTGG AGTATAGCTA CACAGTAACT GCCAGCAACA GCCAAGGGGG TATTTTGAGT 
CCTCTTGTCA AAGATCGAAC CAGCCCCTCA GCACCCTCAG GGATGGAACC TCCAAAATTG 
CAGGCCAGGG GTCCTCAGGA GATCTTAGTG AACTGGGACC CTCCAGTGAG AACAAATGGT 
GATATCATCA ATTATACCCT CTTCATCCGT GAACTATTTG AAAGAGAAAC TAAAATCATA 
CACATAAACA CAACTCATAA TTCTTTTGGT ATGCAGTCAT ATATAGTAAA CCAGCTGAAG 
CCATTTCACA GGTATGAAAT ACGAATTCAA GCGTGCACCA CCCTGGGATG TGCATCAAGT 
GACTGGACAT TCATACAGAC CCCTGAGATT GCACCTTTGA TGCAACCCCC TCCACATCTG 
GAGGTACAAA TGGCTCCAGG AGGATTCCAG CCAACTGTTT CTCTTTTGTG GACAGGACCG 
CTGCAGCCAA ATGGAAAAGT TTTGTATTAC GAATTATACA GAAGACAAAT AGCAACTCAG 
CCTAGAAAAT CCAATCCAGT CCTAATCTAT AACGGAAGCT CAACATCTTT TATAGATTCC 
GAACTATTGC CTTTCACAGA GTATGAGTAT CAGGTCTGGG CAGTGAATTC TGCAGGAAAA 
GCCCCCAGTA GCTGGACATG GTGCAGAACC GGGCCAGCCC CACCAGAAGG TCTCAGAGCC 
CCCACGTTCC ATGTGATCTC TTCTACCCAA GCAGTGGTCA ACATCAGTGC CCCTGGGAAG 
CCCAACGGGA TCGTCAGTCT CTACAGGCTG TTCTCCAGCA GCGCCCATGG GGCTGAGACA 
GTGCTATCCG AAGGCATGGC CACCCAGCAG ACTCTCCATG GCCTTCAAGC CTTCACTAAC 
TACTCTATTG GAGTAGAGGC CTGCACCTGC TTCAACTGTT GCAGCAAAGG ACCGACAGCT 
GAACTGAGAA CCCATCCTGC CCCACCCTCA GGACTGTCCT CTCCACAAAT CGGGACGCTG 
GCCTCAAGGA CGGCCTCCTT CCGGTGGAGT CCCCCCATGT TCCCCAATGG TGTCATTCAC 
AGCTATGAAC TCCAATTCCA CGTGGCTTGC CCTCCTGACT CAGCCCTCCC CTGTACTCCC 
AGCCAAATAG AAACAAAGTA CACGGGGCTG GGGCAGAAAG CCAGCCTTGG GGGTCTCCAG 
CCCTACACCA CATACAAGCT GAGAGTGGTG GCACACAACG AGGTGGGCAG TACGGCTTCC 
GAGTGGATCA GTTTCACCAC CCAAAAAGAA TTGCCTCAGT ACCGAGCCCC ATTTTCGGTG 
GACAGCAATT TGTCTGTGGT GTGTGTGAAC TGGAGTGACA CCTTCCTCCT GAACGGCCAA 
CTGAAGGAGT ACGTGTTAAC CGACGGAGGG CGACGCGTGT ACAGCGGCTT GGACACCACC 
CTCTACATAC CGAGAACGGC GGACAAAACC TTCTTTTTCC AGGTCATCTG CACGACTGAC 
GAAGGAAGTG TTAAGACGCC GTTGATCCAA TATGATACCT CTACTGGACT TGGCTTGGTC 
CTAACAACTC CTGGGAAAAA GAAGGGATCG CGGAGCAAAA GCACAGAGTT CTACAGCGAG 
CTGTGGTTCA TAGTGTTAAT GGCGATGCTG GGCTTGATCT TGTTGGCCAT TTTTCTGTCC 
CTGATACTAC AAAGAAAAAT CCACAAAGAG CCATATATCA GAGAAAGACC TCCCTTGGTA 
CCTCTTCAGA AGAGGATGTC TCCATTGAAT GTTTACCCAC CGGGGGAAAA CCATATGGGG 
TTAGCCGATA CCAAAATTCC CCGGTCTGGG ACACCTGTGA GTATCCGCAG CAACCGGAGT 
GCATGTGTCC TGCGCATCCC GAGTCAAAAC CAAACCAGCC TAACCTACTC CCAGGGTTCT 
CTTCACCGCA GCGTCAGCCA GCTCATGGAC ATTCAAGACA AGAAAGTCTT GATGGACAAC 
TCACTGTGGG AAGCCATCAT GGGCCACAAC AGTGGACTGT ATGTGGATGA AGAGGACCTG 
ATGAACGCCA TCAAGGATTT CAGCTCAGTG ACTAAGGAAC GCACCACATT CACAGACACC 
CACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Usher's Syndrome Type II: A Comparative Study of Genetic Mutations and Vestibular System Evaluation.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery157(5)853-860(2017 Nov)
Magliulo G,Iannella G,Gagliardi S,Iozzo N,Plateroti R,Mariottini A,Torricelli F

UMD-USHbases: a comprehensive set of databases to record and analyse pathogenic mutations and unclassified variants in seven Usher syndrome causing genes.
Human mutation29(8)E76-87(2008 Aug)
Baux D,Faug?re V,Larrieu L,Le Gu?dard-M?reuze S,Hamroun D,B?roud C,Malcolm S,Claustres M,Roux AF

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USH2A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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