VPS51 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS51
Entrez Gene ID	738
Full Name	VPS51, GARP complex subunit
Synonyms	ANG2,ANG3,C11orf2,C11orf3,FFR
General protein information	Preferred Names VPS51, GARP complex subunit Names vacuolar protein sorting-associated protein 51 homolog another new gene 2 protein protein fat-free homolog vacuolar protein sorting 51 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012].
Disorder MIM:	615738

mRNA and Protein(s)

mRNA	Protein	Name
NM_013265.3	NP_037397.2	vacuolar protein sorting-associated protein 51 homolog
NM_013265.4	NP_037397.2	vacuolar protein sorting-associated protein 51 homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VPS51	NP_001095563.1
Drosophila melanogaster (fruit fly)	CG15087	NP_611363.2
Homo sapiens (human)	VPS51	NP_037397.2
Pan troglodytes (chimpanzee)	VPS51	XP_001168116.2
Rattus norvegicus (Norway rat)	Vps51	XP_002728870.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005584	XP_315595.3
Mus musculus (house mouse)	Vps51	NP_001074510.1
Danio rerio (zebrafish)	vps51	NP_001036200.1
Xenopus tropicalis (tropical clawed frog)	vps51	NP_001027521.1
Canis lupus familiaris (dog)	VPS51	XP_533237.2
Caenorhabditis elegans (roundworm)	vps-51	NP_001020972.1

Gene Ontology
Bibliography

Related articles in PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Structural basis for the interaction of the Golgi-Associated Retrograde Protein Complex with the t-SNARE Syntaxin 6.
Abascal-Palacios G, Schindler C, Rojas AL, Bonifacino JS, Hierro A
Structure (London, England : 1993)21(9)1698-706(2013 Sep)

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
P?rez-Victoria FJ, Schindler C, Magad?n JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS
Molecular biology of the cell21(19)3386-95(2010 Oct)

Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1.
Lemmens I, Merregaert J, Van de Ven WJ, Kas K, Zhang CX, Giraud S, Wautot V, Buisson N, De Witte K, Salandre J, Lenoir G, Calender A, Parente F, Quincey D, Courseaux A, Carle GF, Gaudray P, De Wit MJ, Lips CJ, H?ppener JW, Khodaei S, Grant AL, Weber G, Kyt?l? S, Thakker RV
Genomics44(1)94-100(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A dityrosine motif of Ang2 interacts with a highly conserved groove in Syntaxin 6.
Title: Structural basis for the interaction of the Golgi-Associated Retrograde Protein Complex with the t-SNARE Syntaxin 6.

Ang2 is the missing component of the Golgi-associated retrograde protein complex in most eukaryotes.
Title: Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.

The following VPS51 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS51 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16042	NM_013265.4 Latest version!	Homo sapiens VPS51 subunit of GARP complex (VPS51), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu16042	NM_013265.3	Homo sapiens VPS51, GARP complex subunit (VPS51), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS51 ( NM_013265.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037397.2 Homo sapiens VPS51, GARP complex subunit (VPS51), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16042
Clone ID Related Accession (Same CDS sequence)	NM_013265.3 , NM_013265.4
Accession Version	NM_013265.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 51 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX401315.2, BI823833.1, AF024631.2, HY270070.1 and AW007399.1. On Dec 16, 2012 this sequence version replaced NM_013265.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: AF024631.2, SRR1163657.252590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CAGCTGCCGC CGGGCCTAGC CCGGGGTCTG GACCTGGGGA CTCCCCAGAA 
GGGCCCGAGG GGGAGGCTCC GGAGCGTCGG CGGAAGGCGC ACGGGATGCT GAAGCTTTAC 
TACGGCCTCT CGGAAGGGGA GGCGGCGGGA CGCCCCGCGG GGCCCGACCC CCTGGACCCG 
ACTGATCTGA ACGGGGCGCA CTTCGACCCG GAAGTTTACC TAGACAAGCT GCGTAGAGAG 
TGCCCTCTGG CCCAGTTGAT GGACAGTGAG ACGGACATGG TGCGGCAGAT CCGGGCTCTA 
GACAGCGACA TGCAGACCCT GGTCTATGAG AACTACAACA AGTTCATCTC AGCCACAGAC 
ACCATCCGGA AGATGAAGAA CGATTTCCGG AAGATGGAGG ATGAGATGGA CCGGCTGGCC 
ACCAACATGG CAGTGATCAC CGACTTCAGC GCTCGCATCA GCGCCACGCT GCAGGACCGC 
CACGAGCGCA TCACCAAGCT GGCAGGGGTC CACGCGCTGC TGCGGAAGCT GCAGTTCCTC 
TTTGAGCTGC CCTCGCGCCT CACCAAGTGC GTGGAACTGG GCGCCTATGG GCAGGCGGTG 
CGCTACCAGG GCCGCGCGCA GGCCGTGCTG CAGCAGTACC AACACCTGCC CTCGTTCCGC 
GCCATCCAGG ACGACTGCCA GGTCATCACG GCCCGCCTGG CCCAGCAGCT GCGGCAGCGC 
TTTAGGGAGG GCGGCTCAGG CGCCCCGGAG CAGGCAGAGT GCGTGGAGCT GCTGCTGGCC 
CTGGGCGAGC CTGCGGAGGA GCTGTGCGAG GAGTTCCTGG CGCACGCCCG CGGCCGGCTG 
GAGAAGGAGC TGAGAAACCT GGAGGCCGAG CTGGGGCCCT CACCTCCGGC TCCCGACGTG 
TTAGAGTTCA CCGACCATGG AGGCAGTGGC TTCGTGGGCG GCCTCTGCCA GGTGGCGGCG 
GCCTACCAGG AGCTGTTTGC GGCCCAGGGC CCAGCAGGTG CCGAGAAGCT GGCGGCCTTC 
GCCCGGCAGC TGGGCAGCCG CTATTTTGCG CTGGTGGAGC GGCGGCTGGC GCAGGAGCAG 
GGTGGTGGTG ACAACTCACT GCTGGTGCGG GCGCTGGACC GCTTCCACCG GCGCTTGCGG 
GCTCCCGGGG CCCTGCTGGC CGCTGCCGGG CTCGCAGACG CTGCCACGGA GATCGTGGAA 
CGAGTGGCCC GCGAGCGCCT GGGCCACCAC CTGCAGGGTC TCCGGGCGGC CTTCCTGGGC 
TGCCTGACAG ACGTCCGCCA GGCGCTGGCA GCACCTCGCG TGGCTGGGAA GGAGGGCCCT 
GGCCTGGCCG AGTTGCTGGC CAATGTGGCC AGCTCCATCC TGAGCCACAT TAAGGCCTCT 
CTGGCAGCAG TGCACCTTTT CACCGCCAAA GAGGTGTCCT TCTCCAACAA GCCCTACTTC 
CGGGGTGAGT TCTGCAGTCA GGGTGTCCGT GAGGGCCTCA TCGTGGGCTT CGTCCACTCT 
ATGTGCCAGA CGGCTCAGAG CTTCTGCGAC AGCCCTGGGG AGAAGGGGGG TGCCACACCA 
CCTGCCCTGC TCCTGCTGCT CTCCCGCCTC TGCCTGGACT ACGAGACGGC CACCATCTCC 
TACATCCTCA CTCTCACTGA TGAACAGTTT CTGGTGCAGG ATCAGTTCCC AGTGACGCCC 
GTGAGCACGC TGTGTGCAGA GGCCAGGGAA ACGGCGCGGC GGCTGCTGAC CCACTACGTG 
AAGGTGCAGG GCCTGGTCAT ATCACAGATG CTGCGCAAGA GCGTGGAGAC TCGCGACTGG 
CTCAGCACTC TGGAGCCCCG GAATGTGCGG GCCGTCATGA AGCGGGTGGT GGAGGATACC 
ACCGCCATCG ACGTGCAGGT GGGGCTCCTG TACGAAGAGG GTGTTCGCAA GGCCCAGAGC 
AGCGACTCCA GCAAGAGGAC TTTCTCCGTG TACAGCAGCT CTCGGCAGCA GGGCCGCTAC 
GCCCCCAGCT ATACCCCCAG TGCCCCGATG GACACCAACC TCTTGAGCAA TATCCAGAAG 
CTATTCTCTG AACGTATTGA TGTGTTCAGC CCTGTGGAGT TCAACAAGGT GTCGGTGCTG 
ACCGGCATCA TCAAGATCAG CCTGAAGACG CTGCTGGAGT GTGTGCGGCT GCGCACCTTT 
GGGCGCTTCG GGCTGCAGCA GGTGCAAGTG GACTGCCACT TTCTGCAGCT CTACCTGTGG 
CGTTTTGTGG CCGACGAAGA ACTCGTGCAC TTGCTGCTGG ACGAAGTGGT GGCCTCTGCT 
GCCCTGCGCT GCCCAGACCC TGTGCCCATG GAGCCCAGTG TGGTTGAGGT CATCTGCGAG 
CGCGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037397.2
CDS	137..2485
Translation	MAAAAAAGPSPGSGPGDSPEGPEGEAPERRRKAHGMLKLYYGLSEGEAAGRPAGPDPLDPTDLNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNMAVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRAQAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELLLALGEPAEELCEEFLAHARGRLEKELRNLEAELGPSPPAPDVLEFTDHGGSGFVGGLCQVAAAYQELFAAQGPAGAEKLAAFARQLGSRYFALVERRLAQEQGGGDNSLLVRALDRFHRRLRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLGCLTDVRQALAAPRVAGKEGPGLAELLANVASSILSHIKASLAAVHLFTAKEVSFSNKPYFRGEFCSQGVREGLIVGFVHSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQFPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVICERG

Target ORF information:

RefSeq Version	NM_013265.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS51, GARP complex subunit (VPS51), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013265.3

ORF Insert Sequence:

ATGGCGGCGG CAGCTGCCGC CGGGCCTAGC CCGGGGTCTG GACCTGGGGA CTCCCCAGAA 
GGGCCCGAGG GGGAGGCTCC GGAGCGTCGG CGGAAGGCGC ACGGGATGCT GAAGCTTTAC 
TACGGCCTCT CGGAAGGGGA GGCGGCGGGA CGCCCCGCGG GGCCCGACCC CCTGGACCCG 
ACTGATCTGA ACGGGGCGCA CTTCGACCCG GAAGTTTACC TAGACAAGCT GCGTAGAGAG 
TGCCCTCTGG CCCAGTTGAT GGACAGTGAG ACGGACATGG TGCGGCAGAT CCGGGCTCTA 
GACAGCGACA TGCAGACCCT GGTCTATGAG AACTACAACA AGTTCATCTC AGCCACAGAC 
ACCATCCGGA AGATGAAGAA CGATTTCCGG AAGATGGAGG ATGAGATGGA CCGGCTGGCC 
ACCAACATGG CAGTGATCAC CGACTTCAGC GCTCGCATCA GCGCCACGCT GCAGGACCGC 
CACGAGCGCA TCACCAAGCT GGCAGGGGTC CACGCGCTGC TGCGGAAGCT GCAGTTCCTC 
TTTGAGCTGC CCTCGCGCCT CACCAAGTGC GTGGAACTGG GCGCCTATGG GCAGGCGGTG 
CGCTACCAGG GCCGCGCGCA GGCCGTGCTG CAGCAGTACC AACACCTGCC CTCGTTCCGC 
GCCATCCAGG ACGACTGCCA GGTCATCACG GCCCGCCTGG CCCAGCAGCT GCGGCAGCGC 
TTTAGGGAGG GCGGCTCAGG CGCCCCGGAG CAGGCAGAGT GCGTGGAGCT GCTGCTGGCC 
CTGGGCGAGC CTGCGGAGGA GCTGTGCGAG GAGTTCCTGG CGCACGCCCG CGGCCGGCTG 
GAGAAGGAGC TGAGAAACCT GGAGGCCGAG CTGGGGCCCT CACCTCCGGC TCCCGACGTG 
TTAGAGTTCA CCGACCATGG AGGCAGTGGC TTCGTGGGCG GCCTCTGCCA GGTGGCGGCG 
GCCTACCAGG AGCTGTTTGC GGCCCAGGGC CCAGCAGGTG CCGAGAAGCT GGCGGCCTTC 
GCCCGGCAGC TGGGCAGCCG CTATTTTGCG CTGGTGGAGC GGCGGCTGGC GCAGGAGCAG 
GGTGGTGGTG ACAACTCACT GCTGGTGCGG GCGCTGGACC GCTTCCACCG GCGCTTGCGG 
GCTCCCGGGG CCCTGCTGGC CGCTGCCGGG CTCGCAGACG CTGCCACGGA GATCGTGGAA 
CGAGTGGCCC GCGAGCGCCT GGGCCACCAC CTGCAGGGTC TCCGGGCGGC CTTCCTGGGC 
TGCCTGACAG ACGTCCGCCA GGCGCTGGCA GCACCTCGCG TGGCTGGGAA GGAGGGCCCT 
GGCCTGGCCG AGTTGCTGGC CAATGTGGCC AGCTCCATCC TGAGCCACAT TAAGGCCTCT 
CTGGCAGCAG TGCACCTTTT CACCGCCAAA GAGGTGTCCT TCTCCAACAA GCCCTACTTC 
CGGGGTGAGT TCTGCAGTCA GGGTGTCCGT GAGGGCCTCA TCGTGGGCTT CGTCCACTCT 
ATGTGCCAGA CGGCTCAGAG CTTCTGCGAC AGCCCTGGGG AGAAGGGGGG TGCCACACCA 
CCTGCCCTGC TCCTGCTGCT CTCCCGCCTC TGCCTGGACT ACGAGACGGC CACCATCTCC 
TACATCCTCA CTCTCACTGA TGAACAGTTT CTGGTGCAGG ATCAGTTCCC AGTGACGCCC 
GTGAGCACGC TGTGTGCAGA GGCCAGGGAA ACGGCGCGGC GGCTGCTGAC CCACTACGTG 
AAGGTGCAGG GCCTGGTCAT ATCACAGATG CTGCGCAAGA GCGTGGAGAC TCGCGACTGG 
CTCAGCACTC TGGAGCCCCG GAATGTGCGG GCCGTCATGA AGCGGGTGGT GGAGGATACC 
ACCGCCATCG ACGTGCAGGT GGGGCTCCTG TACGAAGAGG GTGTTCGCAA GGCCCAGAGC 
AGCGACTCCA GCAAGAGGAC TTTCTCCGTG TACAGCAGCT CTCGGCAGCA GGGCCGCTAC 
GCCCCCAGCT ATACCCCCAG TGCCCCGATG GACACCAACC TCTTGAGCAA TATCCAGAAG 
CTATTCTCTG AACGTATTGA TGTGTTCAGC CCTGTGGAGT TCAACAAGGT GTCGGTGCTG 
ACCGGCATCA TCAAGATCAG CCTGAAGACG CTGCTGGAGT GTGTGCGGCT GCGCACCTTT 
GGGCGCTTCG GGCTGCAGCA GGTGCAAGTG GACTGCCACT TTCTGCAGCT CTACCTGTGG 
CGTTTTGTGG CCGACGAAGA ACTCGTGCAC TTGCTGCTGG ACGAAGTGGT GGCCTCTGCT 
GCCCTGCGCT GCCCAGACCC TGTGCCCATG GAGCCCAGTG TGGTTGAGGT CATCTGCGAG 
CGCGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS51 ( NM_013265.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037397.2 Homo sapiens VPS51 subunit of GARP complex (VPS51), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16042
Clone ID Related Accession (Same CDS sequence)	NM_013265.3 , NM_013265.4
Accession Version	NM_013265.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 51 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF024631.2, HY270070.1, AW007399.1 and AP003068.3. On Nov 24, 2018 this sequence version replaced NM_013265.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: AF024631.2, SRR3476690.841371.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000279281.8/ ENSP00000279281.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CAGCTGCCGC CGGGCCTAGC CCGGGGTCTG GACCTGGGGA CTCCCCAGAA 
GGGCCCGAGG GGGAGGCTCC GGAGCGTCGG CGGAAGGCGC ACGGGATGCT GAAGCTTTAC 
TACGGCCTCT CGGAAGGGGA GGCGGCGGGA CGCCCCGCGG GGCCCGACCC CCTGGACCCG 
ACTGATCTGA ACGGGGCGCA CTTCGACCCG GAAGTTTACC TAGACAAGCT GCGTAGAGAG 
TGCCCTCTGG CCCAGTTGAT GGACAGTGAG ACGGACATGG TGCGGCAGAT CCGGGCTCTA 
GACAGCGACA TGCAGACCCT GGTCTATGAG AACTACAACA AGTTCATCTC AGCCACAGAC 
ACCATCCGGA AGATGAAGAA CGATTTCCGG AAGATGGAGG ATGAGATGGA CCGGCTGGCC 
ACCAACATGG CAGTGATCAC CGACTTCAGC GCTCGCATCA GCGCCACGCT GCAGGACCGC 
CACGAGCGCA TCACCAAGCT GGCAGGGGTC CACGCGCTGC TGCGGAAGCT GCAGTTCCTC 
TTTGAGCTGC CCTCGCGCCT CACCAAGTGC GTGGAACTGG GCGCCTATGG GCAGGCGGTG 
CGCTACCAGG GCCGCGCGCA GGCCGTGCTG CAGCAGTACC AACACCTGCC CTCGTTCCGC 
GCCATCCAGG ACGACTGCCA GGTCATCACG GCCCGCCTGG CCCAGCAGCT GCGGCAGCGC 
TTTAGGGAGG GCGGCTCAGG CGCCCCGGAG CAGGCAGAGT GCGTGGAGCT GCTGCTGGCC 
CTGGGCGAGC CTGCGGAGGA GCTGTGCGAG GAGTTCCTGG CGCACGCCCG CGGCCGGCTG 
GAGAAGGAGC TGAGAAACCT GGAGGCCGAG CTGGGGCCCT CACCTCCGGC TCCCGACGTG 
TTAGAGTTCA CCGACCATGG AGGCAGTGGC TTCGTGGGCG GCCTCTGCCA GGTGGCGGCG 
GCCTACCAGG AGCTGTTTGC GGCCCAGGGC CCAGCAGGTG CCGAGAAGCT GGCGGCCTTC 
GCCCGGCAGC TGGGCAGCCG CTATTTTGCG CTGGTGGAGC GGCGGCTGGC GCAGGAGCAG 
GGTGGTGGTG ACAACTCACT GCTGGTGCGG GCGCTGGACC GCTTCCACCG GCGCTTGCGG 
GCTCCCGGGG CCCTGCTGGC CGCTGCCGGG CTCGCAGACG CTGCCACGGA GATCGTGGAA 
CGAGTGGCCC GCGAGCGCCT GGGCCACCAC CTGCAGGGTC TCCGGGCGGC CTTCCTGGGC 
TGCCTGACAG ACGTCCGCCA GGCGCTGGCA GCACCTCGCG TGGCTGGGAA GGAGGGCCCT 
GGCCTGGCCG AGTTGCTGGC CAATGTGGCC AGCTCCATCC TGAGCCACAT TAAGGCCTCT 
CTGGCAGCAG TGCACCTTTT CACCGCCAAA GAGGTGTCCT TCTCCAACAA GCCCTACTTC 
CGGGGTGAGT TCTGCAGTCA GGGTGTCCGT GAGGGCCTCA TCGTGGGCTT CGTCCACTCT 
ATGTGCCAGA CGGCTCAGAG CTTCTGCGAC AGCCCTGGGG AGAAGGGGGG TGCCACACCA 
CCTGCCCTGC TCCTGCTGCT CTCCCGCCTC TGCCTGGACT ACGAGACGGC CACCATCTCC 
TACATCCTCA CTCTCACTGA TGAACAGTTT CTGGTGCAGG ATCAGTTCCC AGTGACGCCC 
GTGAGCACGC TGTGTGCAGA GGCCAGGGAA ACGGCGCGGC GGCTGCTGAC CCACTACGTG 
AAGGTGCAGG GCCTGGTCAT ATCACAGATG CTGCGCAAGA GCGTGGAGAC TCGCGACTGG 
CTCAGCACTC TGGAGCCCCG GAATGTGCGG GCCGTCATGA AGCGGGTGGT GGAGGATACC 
ACCGCCATCG ACGTGCAGGT GGGGCTCCTG TACGAAGAGG GTGTTCGCAA GGCCCAGAGC 
AGCGACTCCA GCAAGAGGAC TTTCTCCGTG TACAGCAGCT CTCGGCAGCA GGGCCGCTAC 
GCCCCCAGCT ATACCCCCAG TGCCCCGATG GACACCAACC TCTTGAGCAA TATCCAGAAG 
CTATTCTCTG AACGTATTGA TGTGTTCAGC CCTGTGGAGT TCAACAAGGT GTCGGTGCTG 
ACCGGCATCA TCAAGATCAG CCTGAAGACG CTGCTGGAGT GTGTGCGGCT GCGCACCTTT 
GGGCGCTTCG GGCTGCAGCA GGTGCAAGTG GACTGCCACT TTCTGCAGCT CTACCTGTGG 
CGTTTTGTGG CCGACGAAGA ACTCGTGCAC TTGCTGCTGG ACGAAGTGGT GGCCTCTGCT 
GCCCTGCGCT GCCCAGACCC TGTGCCCATG GAGCCCAGTG TGGTTGAGGT CATCTGCGAG 
CGCGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037397.2
CDS	38..2386
Translation	MAAAAAAGPSPGSGPGDSPEGPEGEAPERRRKAHGMLKLYYGLSEGEAAGRPAGPDPLDPTDLNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNMAVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRAQAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELLLALGEPAEELCEEFLAHARGRLEKELRNLEAELGPSPPAPDVLEFTDHGGSGFVGGLCQVAAAYQELFAAQGPAGAEKLAAFARQLGSRYFALVERRLAQEQGGGDNSLLVRALDRFHRRLRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLGCLTDVRQALAAPRVAGKEGPGLAELLANVASSILSHIKASLAAVHLFTAKEVSFSNKPYFRGEFCSQGVREGLIVGFVHSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQFPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVICERG

Target ORF information:

RefSeq Version	NM_013265.4
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS51 subunit of GARP complex (VPS51), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013265.4

ORF Insert Sequence:

ATGGCGGCGG CAGCTGCCGC CGGGCCTAGC CCGGGGTCTG GACCTGGGGA CTCCCCAGAA 
GGGCCCGAGG GGGAGGCTCC GGAGCGTCGG CGGAAGGCGC ACGGGATGCT GAAGCTTTAC 
TACGGCCTCT CGGAAGGGGA GGCGGCGGGA CGCCCCGCGG GGCCCGACCC CCTGGACCCG 
ACTGATCTGA ACGGGGCGCA CTTCGACCCG GAAGTTTACC TAGACAAGCT GCGTAGAGAG 
TGCCCTCTGG CCCAGTTGAT GGACAGTGAG ACGGACATGG TGCGGCAGAT CCGGGCTCTA 
GACAGCGACA TGCAGACCCT GGTCTATGAG AACTACAACA AGTTCATCTC AGCCACAGAC 
ACCATCCGGA AGATGAAGAA CGATTTCCGG AAGATGGAGG ATGAGATGGA CCGGCTGGCC 
ACCAACATGG CAGTGATCAC CGACTTCAGC GCTCGCATCA GCGCCACGCT GCAGGACCGC 
CACGAGCGCA TCACCAAGCT GGCAGGGGTC CACGCGCTGC TGCGGAAGCT GCAGTTCCTC 
TTTGAGCTGC CCTCGCGCCT CACCAAGTGC GTGGAACTGG GCGCCTATGG GCAGGCGGTG 
CGCTACCAGG GCCGCGCGCA GGCCGTGCTG CAGCAGTACC AACACCTGCC CTCGTTCCGC 
GCCATCCAGG ACGACTGCCA GGTCATCACG GCCCGCCTGG CCCAGCAGCT GCGGCAGCGC 
TTTAGGGAGG GCGGCTCAGG CGCCCCGGAG CAGGCAGAGT GCGTGGAGCT GCTGCTGGCC 
CTGGGCGAGC CTGCGGAGGA GCTGTGCGAG GAGTTCCTGG CGCACGCCCG CGGCCGGCTG 
GAGAAGGAGC TGAGAAACCT GGAGGCCGAG CTGGGGCCCT CACCTCCGGC TCCCGACGTG 
TTAGAGTTCA CCGACCATGG AGGCAGTGGC TTCGTGGGCG GCCTCTGCCA GGTGGCGGCG 
GCCTACCAGG AGCTGTTTGC GGCCCAGGGC CCAGCAGGTG CCGAGAAGCT GGCGGCCTTC 
GCCCGGCAGC TGGGCAGCCG CTATTTTGCG CTGGTGGAGC GGCGGCTGGC GCAGGAGCAG 
GGTGGTGGTG ACAACTCACT GCTGGTGCGG GCGCTGGACC GCTTCCACCG GCGCTTGCGG 
GCTCCCGGGG CCCTGCTGGC CGCTGCCGGG CTCGCAGACG CTGCCACGGA GATCGTGGAA 
CGAGTGGCCC GCGAGCGCCT GGGCCACCAC CTGCAGGGTC TCCGGGCGGC CTTCCTGGGC 
TGCCTGACAG ACGTCCGCCA GGCGCTGGCA GCACCTCGCG TGGCTGGGAA GGAGGGCCCT 
GGCCTGGCCG AGTTGCTGGC CAATGTGGCC AGCTCCATCC TGAGCCACAT TAAGGCCTCT 
CTGGCAGCAG TGCACCTTTT CACCGCCAAA GAGGTGTCCT TCTCCAACAA GCCCTACTTC 
CGGGGTGAGT TCTGCAGTCA GGGTGTCCGT GAGGGCCTCA TCGTGGGCTT CGTCCACTCT 
ATGTGCCAGA CGGCTCAGAG CTTCTGCGAC AGCCCTGGGG AGAAGGGGGG TGCCACACCA 
CCTGCCCTGC TCCTGCTGCT CTCCCGCCTC TGCCTGGACT ACGAGACGGC CACCATCTCC 
TACATCCTCA CTCTCACTGA TGAACAGTTT CTGGTGCAGG ATCAGTTCCC AGTGACGCCC 
GTGAGCACGC TGTGTGCAGA GGCCAGGGAA ACGGCGCGGC GGCTGCTGAC CCACTACGTG 
AAGGTGCAGG GCCTGGTCAT ATCACAGATG CTGCGCAAGA GCGTGGAGAC TCGCGACTGG 
CTCAGCACTC TGGAGCCCCG GAATGTGCGG GCCGTCATGA AGCGGGTGGT GGAGGATACC 
ACCGCCATCG ACGTGCAGGT GGGGCTCCTG TACGAAGAGG GTGTTCGCAA GGCCCAGAGC 
AGCGACTCCA GCAAGAGGAC TTTCTCCGTG TACAGCAGCT CTCGGCAGCA GGGCCGCTAC 
GCCCCCAGCT ATACCCCCAG TGCCCCGATG GACACCAACC TCTTGAGCAA TATCCAGAAG 
CTATTCTCTG AACGTATTGA TGTGTTCAGC CCTGTGGAGT TCAACAAGGT GTCGGTGCTG 
ACCGGCATCA TCAAGATCAG CCTGAAGACG CTGCTGGAGT GTGTGCGGCT GCGCACCTTT 
GGGCGCTTCG GGCTGCAGCA GGTGCAAGTG GACTGCCACT TTCTGCAGCT CTACCTGTGG 
CGTTTTGTGG CCGACGAAGA ACTCGTGCAC TTGCTGCTGG ACGAAGTGGT GGCCTCTGCT 
GCCCTGCGCT GCCCAGACCC TGTGCCCATG GAGCCCAGTG TGGTTGAGGT CATCTGCGAG 
CGCGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Structural basis for the interaction of the Golgi-Associated Retrograde Protein Complex with the t-SNARE Syntaxin 6.
Structure (London, England : 1993)21(9)1698-706(2013 Sep)
Abascal-Palacios G,Schindler C,Rojas AL,Bonifacino JS,Hierro A

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
Molecular biology of the cell21(19)3386-95(2010 Oct)
P?rez-Victoria FJ,Schindler C,Magad?n JG,Mardones GA,Delevoye C,Romao M,Raposo G,Bonifacino JS

Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1.
Genomics44(1)94-100(1997 Aug)
Lemmens I,Merregaert J,Van de Ven WJ,Kas K,Zhang CX,Giraud S,Wautot V,Buisson N,De Witte K,Salandre J,Lenoir G,Calender A,Parente F,Quincey D,Courseaux A,Carle GF,Gaudray P,De Wit MJ,Lips CJ,H?ppener JW,Khodaei S,Grant AL,Weber G,Kyt?l? S,Thakker RV

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VPS51 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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