USP4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP4
Entrez Gene ID	7375
Full Name	ubiquitin specific peptidase 4
Synonyms	UNP,Unph
General protein information	Preferred Names ubiquitin specific peptidase 4 Names ubiquitin carboxyl-terminal hydrolase 4 deubiquitinating enzyme 4 ubiquitin carboxyl-terminal esterase 4 ubiquitin specific peptidase 4 (proto-oncogene) ubiquitin specific protease 4 (proto-oncogene) ubiquitin thioesterase 4 ubiquitin thiolesterase 4 ubiquitin-specific processing protease 4 ubiquitous nuclear protein homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Disorder MIM:	603486

mRNA and Protein(s)

mRNA	Protein	Name
NM_001251877.2	NP_001238806.1	ubiquitin carboxyl-terminal hydrolase 4 isoform c
NM_199443.2	NP_955475.1	ubiquitin carboxyl-terminal hydrolase 4 isoform b
NM_003363.3	NP_003354.2	ubiquitin carboxyl-terminal hydrolase 4 isoform a
NM_199443.3	NP_955475.1	ubiquitin carboxyl-terminal hydrolase 4 isoform b
NM_001251877.1	NP_001238806.1	ubiquitin carboxyl-terminal hydrolase 4 isoform c
NM_003363.4	NP_003354.2	ubiquitin carboxyl-terminal hydrolase 4 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USP4	XP_003432923.1
Gallus gallus (chicken)	USP4	NP_001006134.1
Homo sapiens (human)	USP4	NP_003354.2
	USP4	XP_002802844.1
Bos taurus (cattle)	USP4	NP_001093789.1
Rattus norvegicus (Norway rat)	Usp4	NP_001128484.1
Xenopus tropicalis (tropical clawed frog)	usp4	XP_002936515.1
Pan troglodytes (chimpanzee)	USP4	XP_001163445.1
Danio rerio (zebrafish)	usp4	XP_002662556.2
Mus musculus (house mouse)	Usp4	NP_035808.2
Caenorhabditis elegans (roundworm)	H34C03.2	NP_501035.1
Arabidopsis thaliana (thale cress)	UBP5	NP_565944.1

Gene Ontology

The following USP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19305	NM_003363.4 Latest version!	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu12326	NM_001251877.2 Latest version!	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02821	NM_199443.3 Latest version!	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu02821	NM_199443.2	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu12326	NM_001251877.1	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu19305	NM_003363.3	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP4 ( NM_003363.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003354.2 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.

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CloneID	OHu19305
Clone ID Related Accession (Same CDS sequence)	NM_003363.3 , NM_003363.4
Accession Version	NM_003363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC125131.1, BC125130.1, AI887410.1, DB534804.1 and DA267618.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_003363.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.1018.1, SRR1803615.162605.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265560.9/ ENSP00000265560.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAG CACCTGTGGG ATGCACAGTT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTTT CTGCTTCGTA TAATTGTCAG GAGCCACCAT CCTCTCATAT ACAACCTGGG 
CTCTGTGGAC TTGGAAACCT GGGAAACACC TGCTTCATGA ACTCCGCTTT GCAGTGTTTG 
AGCAACACTG CACCACTGAC TGACTACTTT CTCAAAGATG AGTATGAAGC CGAAATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAA ATTGCAGAAG CCTATGCTGA ACTCATTAAG 
CAGATGTGGT CTGGAAGGGA CGCCCATGTG GCACCTCGCA TGTTCAAAAC TCAAGTAGGA 
CGTTTTGCTC CTCAATTTTC TGGCTACCAG CAACAAGATT CTCAGGAGCT GCTGGCCTTT 
CTTCTAGATG GATTGCATGA AGATCTGAAC CGGGTAAAGA AAAAGCCCTA CTTGGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCACAGG 
TTGAGGAATG ATTCTGTGAT TGTGGATACT TTCCATGGCC TCTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACGCTGCCA 
CTGCCCTTGA AGAAAGATCG AGTTATGGAG GTTTTCCTGG TTCCTGCTGA CCCTCACTGC 
AGACCTACTC AGTACCGTGT GACTGTGCCG CTGATGGGGG CTGTGTCCGA CCTGTGCGAG 
GCTCTCTCCA GGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCGCAGA TGTGTATAAT 
CACCGATTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTCG TGTACGAGGT CTGCAGCACT TCCGTGGATG GCTCGGAATG TGTCACGCTT 
CCAGTCTACT TCAGGGAGAG GAAGTCCAGG CCATCAAGCA CTTCCTCCGC ATCAGCGCTA 
TATGGGCAGC CACTATTGCT TTCTGTCCCC AAGCACAAGT TAACCCTTGA GTCTTTGTAC 
CAGGCTGTTT GTGATCGTAT CAGCCGCTAT GTGAAACAGC CTTTACCTGA TGAGTTTGGC 
AGCTCACCCT TGGAGCCAGG GGCCTGCAAT GGCTCCAGGA ACAGCTGTGA AGGAGAAGAT 
GAGGAAGAAA TGGAGCATCA GGAAGAAGGC AAAGAGCAGC TTTCAGAAAC AGAAGGCAGT 
GGGGAAGATG AGCCAGGAAA TGACCCCAGT GAGACCACCC AAAAGAAGAT CAAAGGCCAG 
CCCTGCCCAA AAAGGCTTTT TACCTTCAGT CTTGTGAACT CCTATGGAAC AGCTGACATA 
AATTCACTTG CAGCTGATGG AAAACTACTT AAACTCAACT CTCGATCTAC ACTGGCCATG 
GATTGGGACA GTGAAACTCG GAGACTTTAC TATGATGAGC AAGAATCTGA GGCCTACGAG 
AAGCATGTGA GCATGTTGCA GCCTCAGAAG AAGAAGAAGA CCACAGTGGC CCTGAGAGAC 
TGCATCGAGC TCTTCACCAC CATGGAGACC CTTGGGGAGC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCATCAACA GGCCACAAAA AAGTTTGACC TATGGTCCTT GCCCAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AACAGATACT GGAGGGATAA GCTCGACACA 
GTCGTAGAAT TCCCAATCAG AGGGCTGAAC ATGTCCGAGT TTGTCTGTAA CCTGTCAGCA 
AGGCCTTATG TGTACGACCT CATTGCCGTG TCCAATCATT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATATGCGAA GAACAAACTG AATGGTAAAT GGTATTACTT TGATGATAGC 
AACGTGTCCC TGGCCTCTGA GGATCAGATA GTGACTAAAG CAGCTTATGT GCTATTTTAC 
CAACGTCGAG ATGATGAATT TTATAAGACA CCTTCACTTA GCAGTTCTGG TTCCTCTGAT 
GGAGGGACAC GACCAAGCAG CTCTCAGCAG GGCTTTGGGG ATGATGAGGC TTGCAGCATG 
GACACCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003354.2
CDS	30..2921
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN

Target ORF information:

RefSeq Version	NM_003363.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003363.4

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAG CACCTGTGGG ATGCACAGTT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTTT CTGCTTCGTA TAATTGTCAG GAGCCACCAT CCTCTCATAT ACAACCTGGG 
CTCTGTGGAC TTGGAAACCT GGGAAACACC TGCTTCATGA ACTCCGCTTT GCAGTGTTTG 
AGCAACACTG CACCACTGAC TGACTACTTT CTCAAAGATG AGTATGAAGC CGAAATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAA ATTGCAGAAG CCTATGCTGA ACTCATTAAG 
CAGATGTGGT CTGGAAGGGA CGCCCATGTG GCACCTCGCA TGTTCAAAAC TCAAGTAGGA 
CGTTTTGCTC CTCAATTTTC TGGCTACCAG CAACAAGATT CTCAGGAGCT GCTGGCCTTT 
CTTCTAGATG GATTGCATGA AGATCTGAAC CGGGTAAAGA AAAAGCCCTA CTTGGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCACAGG 
TTGAGGAATG ATTCTGTGAT TGTGGATACT TTCCATGGCC TCTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACGCTGCCA 
CTGCCCTTGA AGAAAGATCG AGTTATGGAG GTTTTCCTGG TTCCTGCTGA CCCTCACTGC 
AGACCTACTC AGTACCGTGT GACTGTGCCG CTGATGGGGG CTGTGTCCGA CCTGTGCGAG 
GCTCTCTCCA GGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCGCAGA TGTGTATAAT 
CACCGATTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTCG TGTACGAGGT CTGCAGCACT TCCGTGGATG GCTCGGAATG TGTCACGCTT 
CCAGTCTACT TCAGGGAGAG GAAGTCCAGG CCATCAAGCA CTTCCTCCGC ATCAGCGCTA 
TATGGGCAGC CACTATTGCT TTCTGTCCCC AAGCACAAGT TAACCCTTGA GTCTTTGTAC 
CAGGCTGTTT GTGATCGTAT CAGCCGCTAT GTGAAACAGC CTTTACCTGA TGAGTTTGGC 
AGCTCACCCT TGGAGCCAGG GGCCTGCAAT GGCTCCAGGA ACAGCTGTGA AGGAGAAGAT 
GAGGAAGAAA TGGAGCATCA GGAAGAAGGC AAAGAGCAGC TTTCAGAAAC AGAAGGCAGT 
GGGGAAGATG AGCCAGGAAA TGACCCCAGT GAGACCACCC AAAAGAAGAT CAAAGGCCAG 
CCCTGCCCAA AAAGGCTTTT TACCTTCAGT CTTGTGAACT CCTATGGAAC AGCTGACATA 
AATTCACTTG CAGCTGATGG AAAACTACTT AAACTCAACT CTCGATCTAC ACTGGCCATG 
GATTGGGACA GTGAAACTCG GAGACTTTAC TATGATGAGC AAGAATCTGA GGCCTACGAG 
AAGCATGTGA GCATGTTGCA GCCTCAGAAG AAGAAGAAGA CCACAGTGGC CCTGAGAGAC 
TGCATCGAGC TCTTCACCAC CATGGAGACC CTTGGGGAGC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCATCAACA GGCCACAAAA AAGTTTGACC TATGGTCCTT GCCCAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AACAGATACT GGAGGGATAA GCTCGACACA 
GTCGTAGAAT TCCCAATCAG AGGGCTGAAC ATGTCCGAGT TTGTCTGTAA CCTGTCAGCA 
AGGCCTTATG TGTACGACCT CATTGCCGTG TCCAATCATT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATATGCGAA GAACAAACTG AATGGTAAAT GGTATTACTT TGATGATAGC 
AACGTGTCCC TGGCCTCTGA GGATCAGATA GTGACTAAAG CAGCTTATGT GCTATTTTAC 
CAACGTCGAG ATGATGAATT TTATAAGACA CCTTCACTTA GCAGTTCTGG TTCCTCTGAT 
GGAGGGACAC GACCAAGCAG CTCTCAGCAG GGCTTTGGGG ATGATGAGGC TTGCAGCATG 
GACACCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( NM_001251877.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238806.1 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12326
Clone ID Related Accession (Same CDS sequence)	NM_001251877.2 , NM_001251877.1
Accession Version	NM_001251877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC121247.2 and BC068017.1. On May 31, 2019 this sequence version replaced NM_001251877.1. Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform c, which is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068017.1, BI908596.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAAGTTT CGTTCTTTTT GCCCAGGCTG 
GAGTGCAATG GCGCGATCTT GGCTCACTGC AACTTCTGCC TCCCGGGTTC AAGCAATTCT 
CCTGCCTCAG CTTCCCGAGT AGCGCCATCA CATCTGGCTA ATTTTTTTTT TTTTGAGATG 
GAGTCTCACT CTGTCACCAA GCTGGAGTGC GGTGGTGCAG TCTCGGCTTA CTCCCGGGTT 
CAAGTGATGC TTCTGCCCCA GCCTCCCGAG TGGCTGGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238806.1
CDS	30..971
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKVSFFLPRLECNGAILAHCNFCLPGSSNSPASASRVAPSHLANFFFFEMESHSVTKLECGGAVSAYSRVQVMLLPQPPEWLG

Target ORF information:

RefSeq Version	NM_001251877.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251877.2

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAAGTTT CGTTCTTTTT GCCCAGGCTG 
GAGTGCAATG GCGCGATCTT GGCTCACTGC AACTTCTGCC TCCCGGGTTC AAGCAATTCT 
CCTGCCTCAG CTTCCCGAGT AGCGCCATCA CATCTGGCTA ATTTTTTTTT TTTTGAGATG 
GAGTCTCACT CTGTCACCAA GCTGGAGTGC GGTGGTGCAG TCTCGGCTTA CTCCCGGGTT 
CAAGTGATGC TTCTGCCCCA GCCTCCCGAG TGGCTGGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( NM_001251877.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238806.1 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12326
Clone ID Related Accession (Same CDS sequence)	NM_001251877.2 , NM_001251877.1
Accession Version	NM_001251877.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-10
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC121247.2 and BC068017.1. Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform c, which is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068017.1, BI908596.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAAGTTT CGTTCTTTTT GCCCAGGCTG 
GAGTGCAATG GCGCGATCTT GGCTCACTGC AACTTCTGCC TCCCGGGTTC AAGCAATTCT 
CCTGCCTCAG CTTCCCGAGT AGCGCCATCA CATCTGGCTA ATTTTTTTTT TTTTGAGATG 
GAGTCTCACT CTGTCACCAA GCTGGAGTGC GGTGGTGCAG TCTCGGCTTA CTCCCGGGTT 
CAAGTGATGC TTCTGCCCCA GCCTCCCGAG TGGCTGGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238806.1
CDS	80..1021
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKVSFFLPRLECNGAILAHCNFCLPGSSNSPASASRVAPSHLANFFFFEMESHSVTKLECGGAVSAYSRVQVMLLPQPPEWLG

Target ORF information:

RefSeq Version	NM_001251877.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251877.1

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAAGTTT CGTTCTTTTT GCCCAGGCTG 
GAGTGCAATG GCGCGATCTT GGCTCACTGC AACTTCTGCC TCCCGGGTTC AAGCAATTCT 
CCTGCCTCAG CTTCCCGAGT AGCGCCATCA CATCTGGCTA ATTTTTTTTT TTTTGAGATG 
GAGTCTCACT CTGTCACCAA GCTGGAGTGC GGTGGTGCAG TCTCGGCTTA CTCCCGGGTT 
CAAGTGATGC TTCTGCCCCA GCCTCCCGAG TGGCTGGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( NM_199443.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_955475.1 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02821
Clone ID Related Accession (Same CDS sequence)	NM_199443.2 , NM_199443.3
Accession Version	NM_199443.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-10
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717217.1, AF017306.1, BC125130.1, AI887410.1, DB534804.1 and DA267618.1. On Aug 14, 2008 this sequence version replaced NM_199443.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.116831.1, SRR1660803.165120.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAATGGAT CTGGCTTTTC TGCTTCGTAT 
AATTGTCAGG AGCCACCATC CTCTCATATA CAACCTGGGC TCTGTGGACT TGGAAACCTG 
GGAAACACCT GCTTCATGAA CTCCGCTTTG CAGTGTTTGA GCAACACTGC ACCACTGACT 
GACTACTTTC TCAAAGATGA GTATGAAGCC GAAATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAAA TTGCAGAAGC CTATGCTGAA CTCATTAAGC AGATGTGGTC TGGAAGGGAC 
GCCCATGTGG CACCTCGCAT GTTCAAAACT CAAGTAGGAC GTTTTGCTCC TCAATTTTCT 
GGCTACCAGC AACAAGATTC TCAGGAGCTG CTGGCCTTTC TTCTAGATGG ATTGCATGAA 
GATCTGAACC GGGTAAAGAA AAAGCCCTAC TTGGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCACAGGT TGAGGAATGA TTCTGTGATT 
GTGGATACTT TCCATGGCCT CTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACGCTGCCAC TGCCCTTGAA GAAAGATCGA 
GTTATGGAGG TTTTCCTGGT TCCTGCTGAC CCTCACTGCA GACCTACTCA GTACCGTGTG 
ACTGTGCCGC TGATGGGGGC TGTGTCCGAC CTGTGCGAGG CTCTCTCCAG GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCGCAGAT GTGTATAATC ACCGATTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTCGT GTACGAGGTC 
TGCAGCACTT CCGTGGATGG CTCGGAATGT GTCACGCTTC CAGTCTACTT CAGGGAGAGG 
AAGTCCAGGC CATCAAGCAC TTCCTCCGCA TCAGCGCTAT ATGGGCAGCC ACTATTGCTT 
TCTGTCCCCA AGCACAAGTT AACCCTTGAG TCTTTGTACC AGGCTGTTTG TGATCGTATC 
AGCCGCTATG TGAAACAGCC TTTACCTGAT GAGTTTGGCA GCTCACCCTT GGAGCCAGGG 
GCCTGCAATG GCTCCAGGAA CAGCTGTGAA GGAGAAGATG AGGAAGAAAT GGAGCATCAG 
GAAGAAGGCA AAGAGCAGCT TTCAGAAACA GAAGGCAGTG GGGAAGATGA GCCAGGAAAT 
GACCCCAGTG AGACCACCCA AAAGAAGATC AAAGGCCAGC CCTGCCCAAA AAGGCTTTTT 
ACCTTCAGTC TTGTGAACTC CTATGGAACA GCTGACATAA ATTCACTTGC AGCTGATGGA 
AAACTACTTA AACTCAACTC TCGATCTACA CTGGCCATGG ATTGGGACAG TGAAACTCGG 
AGACTTTACT ATGATGAGCA AGAATCTGAG GCCTACGAGA AGCATGTGAG CATGTTGCAG 
CCTCAGAAGA AGAAGAAGAC CACAGTGGCC CTGAGAGACT GCATCGAGCT CTTCACCACC 
ATGGAGACCC TTGGGGAGCA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCATCAACAG 
GCCACAAAAA AGTTTGACCT ATGGTCCTTG CCCAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ACAGATACTG GAGGGATAAG CTCGACACAG TCGTAGAATT CCCAATCAGA 
GGGCTGAACA TGTCCGAGTT TGTCTGTAAC CTGTCAGCAA GGCCTTATGT GTACGACCTC 
ATTGCCGTGT CCAATCATTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATATGCGAAG 
AACAAACTGA ATGGTAAATG GTATTACTTT GATGATAGCA ACGTGTCCCT GGCCTCTGAG 
GATCAGATAG TGACTAAAGC AGCTTATGTG CTATTTTACC AACGTCGAGA TGATGAATTT 
TATAAGACAC CTTCACTTAG CAGTTCTGGT TCCTCTGATG GAGGGACACG ACCAAGCAGC 
TCTCAGCAGG GCTTTGGGGA TGATGAGGCT TGCAGCATGG ACACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955475.1
CDS	80..2830
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSNGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN

Target ORF information:

RefSeq Version	NM_199443.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199443.2

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAATGGAT CTGGCTTTTC TGCTTCGTAT 
AATTGTCAGG AGCCACCATC CTCTCATATA CAACCTGGGC TCTGTGGACT TGGAAACCTG 
GGAAACACCT GCTTCATGAA CTCCGCTTTG CAGTGTTTGA GCAACACTGC ACCACTGACT 
GACTACTTTC TCAAAGATGA GTATGAAGCC GAAATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAAA TTGCAGAAGC CTATGCTGAA CTCATTAAGC AGATGTGGTC TGGAAGGGAC 
GCCCATGTGG CACCTCGCAT GTTCAAAACT CAAGTAGGAC GTTTTGCTCC TCAATTTTCT 
GGCTACCAGC AACAAGATTC TCAGGAGCTG CTGGCCTTTC TTCTAGATGG ATTGCATGAA 
GATCTGAACC GGGTAAAGAA AAAGCCCTAC TTGGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCACAGGT TGAGGAATGA TTCTGTGATT 
GTGGATACTT TCCATGGCCT CTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACGCTGCCAC TGCCCTTGAA GAAAGATCGA 
GTTATGGAGG TTTTCCTGGT TCCTGCTGAC CCTCACTGCA GACCTACTCA GTACCGTGTG 
ACTGTGCCGC TGATGGGGGC TGTGTCCGAC CTGTGCGAGG CTCTCTCCAG GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCGCAGAT GTGTATAATC ACCGATTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTCGT GTACGAGGTC 
TGCAGCACTT CCGTGGATGG CTCGGAATGT GTCACGCTTC CAGTCTACTT CAGGGAGAGG 
AAGTCCAGGC CATCAAGCAC TTCCTCCGCA TCAGCGCTAT ATGGGCAGCC ACTATTGCTT 
TCTGTCCCCA AGCACAAGTT AACCCTTGAG TCTTTGTACC AGGCTGTTTG TGATCGTATC 
AGCCGCTATG TGAAACAGCC TTTACCTGAT GAGTTTGGCA GCTCACCCTT GGAGCCAGGG 
GCCTGCAATG GCTCCAGGAA CAGCTGTGAA GGAGAAGATG AGGAAGAAAT GGAGCATCAG 
GAAGAAGGCA AAGAGCAGCT TTCAGAAACA GAAGGCAGTG GGGAAGATGA GCCAGGAAAT 
GACCCCAGTG AGACCACCCA AAAGAAGATC AAAGGCCAGC CCTGCCCAAA AAGGCTTTTT 
ACCTTCAGTC TTGTGAACTC CTATGGAACA GCTGACATAA ATTCACTTGC AGCTGATGGA 
AAACTACTTA AACTCAACTC TCGATCTACA CTGGCCATGG ATTGGGACAG TGAAACTCGG 
AGACTTTACT ATGATGAGCA AGAATCTGAG GCCTACGAGA AGCATGTGAG CATGTTGCAG 
CCTCAGAAGA AGAAGAAGAC CACAGTGGCC CTGAGAGACT GCATCGAGCT CTTCACCACC 
ATGGAGACCC TTGGGGAGCA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCATCAACAG 
GCCACAAAAA AGTTTGACCT ATGGTCCTTG CCCAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ACAGATACTG GAGGGATAAG CTCGACACAG TCGTAGAATT CCCAATCAGA 
GGGCTGAACA TGTCCGAGTT TGTCTGTAAC CTGTCAGCAA GGCCTTATGT GTACGACCTC 
ATTGCCGTGT CCAATCATTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATATGCGAAG 
AACAAACTGA ATGGTAAATG GTATTACTTT GATGATAGCA ACGTGTCCCT GGCCTCTGAG 
GATCAGATAG TGACTAAAGC AGCTTATGTG CTATTTTACC AACGTCGAGA TGATGAATTT 
TATAAGACAC CTTCACTTAG CAGTTCTGGT TCCTCTGATG GAGGGACACG ACCAAGCAGC 
TCTCAGCAGG GCTTTGGGGA TGATGAGGCT TGCAGCATGG ACACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( NM_199443.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_955475.1 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02821
Clone ID Related Accession (Same CDS sequence)	NM_199443.2 , NM_199443.3
Accession Version	NM_199443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717217.1, AF017306.1, BC125130.1, AI887410.1, DB534804.1 and DA267618.1. On May 31, 2019 this sequence version replaced NM_199443.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.116831.1, SRR1803616.246375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAATGGAT CTGGCTTTTC TGCTTCGTAT 
AATTGTCAGG AGCCACCATC CTCTCATATA CAACCTGGGC TCTGTGGACT TGGAAACCTG 
GGAAACACCT GCTTCATGAA CTCCGCTTTG CAGTGTTTGA GCAACACTGC ACCACTGACT 
GACTACTTTC TCAAAGATGA GTATGAAGCC GAAATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAAA TTGCAGAAGC CTATGCTGAA CTCATTAAGC AGATGTGGTC TGGAAGGGAC 
GCCCATGTGG CACCTCGCAT GTTCAAAACT CAAGTAGGAC GTTTTGCTCC TCAATTTTCT 
GGCTACCAGC AACAAGATTC TCAGGAGCTG CTGGCCTTTC TTCTAGATGG ATTGCATGAA 
GATCTGAACC GGGTAAAGAA AAAGCCCTAC TTGGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCACAGGT TGAGGAATGA TTCTGTGATT 
GTGGATACTT TCCATGGCCT CTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACGCTGCCAC TGCCCTTGAA GAAAGATCGA 
GTTATGGAGG TTTTCCTGGT TCCTGCTGAC CCTCACTGCA GACCTACTCA GTACCGTGTG 
ACTGTGCCGC TGATGGGGGC TGTGTCCGAC CTGTGCGAGG CTCTCTCCAG GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCGCAGAT GTGTATAATC ACCGATTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTCGT GTACGAGGTC 
TGCAGCACTT CCGTGGATGG CTCGGAATGT GTCACGCTTC CAGTCTACTT CAGGGAGAGG 
AAGTCCAGGC CATCAAGCAC TTCCTCCGCA TCAGCGCTAT ATGGGCAGCC ACTATTGCTT 
TCTGTCCCCA AGCACAAGTT AACCCTTGAG TCTTTGTACC AGGCTGTTTG TGATCGTATC 
AGCCGCTATG TGAAACAGCC TTTACCTGAT GAGTTTGGCA GCTCACCCTT GGAGCCAGGG 
GCCTGCAATG GCTCCAGGAA CAGCTGTGAA GGAGAAGATG AGGAAGAAAT GGAGCATCAG 
GAAGAAGGCA AAGAGCAGCT TTCAGAAACA GAAGGCAGTG GGGAAGATGA GCCAGGAAAT 
GACCCCAGTG AGACCACCCA AAAGAAGATC AAAGGCCAGC CCTGCCCAAA AAGGCTTTTT 
ACCTTCAGTC TTGTGAACTC CTATGGAACA GCTGACATAA ATTCACTTGC AGCTGATGGA 
AAACTACTTA AACTCAACTC TCGATCTACA CTGGCCATGG ATTGGGACAG TGAAACTCGG 
AGACTTTACT ATGATGAGCA AGAATCTGAG GCCTACGAGA AGCATGTGAG CATGTTGCAG 
CCTCAGAAGA AGAAGAAGAC CACAGTGGCC CTGAGAGACT GCATCGAGCT CTTCACCACC 
ATGGAGACCC TTGGGGAGCA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCATCAACAG 
GCCACAAAAA AGTTTGACCT ATGGTCCTTG CCCAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ACAGATACTG GAGGGATAAG CTCGACACAG TCGTAGAATT CCCAATCAGA 
GGGCTGAACA TGTCCGAGTT TGTCTGTAAC CTGTCAGCAA GGCCTTATGT GTACGACCTC 
ATTGCCGTGT CCAATCATTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATATGCGAAG 
AACAAACTGA ATGGTAAATG GTATTACTTT GATGATAGCA ACGTGTCCCT GGCCTCTGAG 
GATCAGATAG TGACTAAAGC AGCTTATGTG CTATTTTACC AACGTCGAGA TGATGAATTT 
TATAAGACAC CTTCACTTAG CAGTTCTGGT TCCTCTGATG GAGGGACACG ACCAAGCAGC 
TCTCAGCAGG GCTTTGGGGA TGATGAGGCT TGCAGCATGG ACACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955475.1
CDS	30..2780
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSNGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN

Target ORF information:

RefSeq Version	NM_199443.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199443.3

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAATGGAT CTGGCTTTTC TGCTTCGTAT 
AATTGTCAGG AGCCACCATC CTCTCATATA CAACCTGGGC TCTGTGGACT TGGAAACCTG 
GGAAACACCT GCTTCATGAA CTCCGCTTTG CAGTGTTTGA GCAACACTGC ACCACTGACT 
GACTACTTTC TCAAAGATGA GTATGAAGCC GAAATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAAA TTGCAGAAGC CTATGCTGAA CTCATTAAGC AGATGTGGTC TGGAAGGGAC 
GCCCATGTGG CACCTCGCAT GTTCAAAACT CAAGTAGGAC GTTTTGCTCC TCAATTTTCT 
GGCTACCAGC AACAAGATTC TCAGGAGCTG CTGGCCTTTC TTCTAGATGG ATTGCATGAA 
GATCTGAACC GGGTAAAGAA AAAGCCCTAC TTGGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCACAGGT TGAGGAATGA TTCTGTGATT 
GTGGATACTT TCCATGGCCT CTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACGCTGCCAC TGCCCTTGAA GAAAGATCGA 
GTTATGGAGG TTTTCCTGGT TCCTGCTGAC CCTCACTGCA GACCTACTCA GTACCGTGTG 
ACTGTGCCGC TGATGGGGGC TGTGTCCGAC CTGTGCGAGG CTCTCTCCAG GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCGCAGAT GTGTATAATC ACCGATTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTCGT GTACGAGGTC 
TGCAGCACTT CCGTGGATGG CTCGGAATGT GTCACGCTTC CAGTCTACTT CAGGGAGAGG 
AAGTCCAGGC CATCAAGCAC TTCCTCCGCA TCAGCGCTAT ATGGGCAGCC ACTATTGCTT 
TCTGTCCCCA AGCACAAGTT AACCCTTGAG TCTTTGTACC AGGCTGTTTG TGATCGTATC 
AGCCGCTATG TGAAACAGCC TTTACCTGAT GAGTTTGGCA GCTCACCCTT GGAGCCAGGG 
GCCTGCAATG GCTCCAGGAA CAGCTGTGAA GGAGAAGATG AGGAAGAAAT GGAGCATCAG 
GAAGAAGGCA AAGAGCAGCT TTCAGAAACA GAAGGCAGTG GGGAAGATGA GCCAGGAAAT 
GACCCCAGTG AGACCACCCA AAAGAAGATC AAAGGCCAGC CCTGCCCAAA AAGGCTTTTT 
ACCTTCAGTC TTGTGAACTC CTATGGAACA GCTGACATAA ATTCACTTGC AGCTGATGGA 
AAACTACTTA AACTCAACTC TCGATCTACA CTGGCCATGG ATTGGGACAG TGAAACTCGG 
AGACTTTACT ATGATGAGCA AGAATCTGAG GCCTACGAGA AGCATGTGAG CATGTTGCAG 
CCTCAGAAGA AGAAGAAGAC CACAGTGGCC CTGAGAGACT GCATCGAGCT CTTCACCACC 
ATGGAGACCC TTGGGGAGCA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCATCAACAG 
GCCACAAAAA AGTTTGACCT ATGGTCCTTG CCCAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ACAGATACTG GAGGGATAAG CTCGACACAG TCGTAGAATT CCCAATCAGA 
GGGCTGAACA TGTCCGAGTT TGTCTGTAAC CTGTCAGCAA GGCCTTATGT GTACGACCTC 
ATTGCCGTGT CCAATCATTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATATGCGAAG 
AACAAACTGA ATGGTAAATG GTATTACTTT GATGATAGCA ACGTGTCCCT GGCCTCTGAG 
GATCAGATAG TGACTAAAGC AGCTTATGTG CTATTTTACC AACGTCGAGA TGATGAATTT 
TATAAGACAC CTTCACTTAG CAGTTCTGGT TCCTCTGATG GAGGGACACG ACCAAGCAGC 
TCTCAGCAGG GCTTTGGGGA TGATGAGGCT TGCAGCATGG ACACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( NM_003363.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003354.2 Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.

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CloneID	OHu19305
Clone ID Related Accession (Same CDS sequence)	NM_003363.3 , NM_003363.4
Accession Version	NM_003363.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717217.1, BC125131.1, BC125130.1, AI887410.1, DB534804.1 and DA267618.1. This sequence is a reference standard in the RefSeqGene project. On Aug 14, 2008 this sequence version replaced NM_003363.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.265832.1, SRR1660805.217439.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAG CACCTGTGGG ATGCACAGTT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTTT CTGCTTCGTA TAATTGTCAG GAGCCACCAT CCTCTCATAT ACAACCTGGG 
CTCTGTGGAC TTGGAAACCT GGGAAACACC TGCTTCATGA ACTCCGCTTT GCAGTGTTTG 
AGCAACACTG CACCACTGAC TGACTACTTT CTCAAAGATG AGTATGAAGC CGAAATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAA ATTGCAGAAG CCTATGCTGA ACTCATTAAG 
CAGATGTGGT CTGGAAGGGA CGCCCATGTG GCACCTCGCA TGTTCAAAAC TCAAGTAGGA 
CGTTTTGCTC CTCAATTTTC TGGCTACCAG CAACAAGATT CTCAGGAGCT GCTGGCCTTT 
CTTCTAGATG GATTGCATGA AGATCTGAAC CGGGTAAAGA AAAAGCCCTA CTTGGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCACAGG 
TTGAGGAATG ATTCTGTGAT TGTGGATACT TTCCATGGCC TCTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACGCTGCCA 
CTGCCCTTGA AGAAAGATCG AGTTATGGAG GTTTTCCTGG TTCCTGCTGA CCCTCACTGC 
AGACCTACTC AGTACCGTGT GACTGTGCCG CTGATGGGGG CTGTGTCCGA CCTGTGCGAG 
GCTCTCTCCA GGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCGCAGA TGTGTATAAT 
CACCGATTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTCG TGTACGAGGT CTGCAGCACT TCCGTGGATG GCTCGGAATG TGTCACGCTT 
CCAGTCTACT TCAGGGAGAG GAAGTCCAGG CCATCAAGCA CTTCCTCCGC ATCAGCGCTA 
TATGGGCAGC CACTATTGCT TTCTGTCCCC AAGCACAAGT TAACCCTTGA GTCTTTGTAC 
CAGGCTGTTT GTGATCGTAT CAGCCGCTAT GTGAAACAGC CTTTACCTGA TGAGTTTGGC 
AGCTCACCCT TGGAGCCAGG GGCCTGCAAT GGCTCCAGGA ACAGCTGTGA AGGAGAAGAT 
GAGGAAGAAA TGGAGCATCA GGAAGAAGGC AAAGAGCAGC TTTCAGAAAC AGAAGGCAGT 
GGGGAAGATG AGCCAGGAAA TGACCCCAGT GAGACCACCC AAAAGAAGAT CAAAGGCCAG 
CCCTGCCCAA AAAGGCTTTT TACCTTCAGT CTTGTGAACT CCTATGGAAC AGCTGACATA 
AATTCACTTG CAGCTGATGG AAAACTACTT AAACTCAACT CTCGATCTAC ACTGGCCATG 
GATTGGGACA GTGAAACTCG GAGACTTTAC TATGATGAGC AAGAATCTGA GGCCTACGAG 
AAGCATGTGA GCATGTTGCA GCCTCAGAAG AAGAAGAAGA CCACAGTGGC CCTGAGAGAC 
TGCATCGAGC TCTTCACCAC CATGGAGACC CTTGGGGAGC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCATCAACA GGCCACAAAA AAGTTTGACC TATGGTCCTT GCCCAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AACAGATACT GGAGGGATAA GCTCGACACA 
GTCGTAGAAT TCCCAATCAG AGGGCTGAAC ATGTCCGAGT TTGTCTGTAA CCTGTCAGCA 
AGGCCTTATG TGTACGACCT CATTGCCGTG TCCAATCATT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATATGCGAA GAACAAACTG AATGGTAAAT GGTATTACTT TGATGATAGC 
AACGTGTCCC TGGCCTCTGA GGATCAGATA GTGACTAAAG CAGCTTATGT GCTATTTTAC 
CAACGTCGAG ATGATGAATT TTATAAGACA CCTTCACTTA GCAGTTCTGG TTCCTCTGAT 
GGAGGGACAC GACCAAGCAG CTCTCAGCAG GGCTTTGGGG ATGATGAGGC TTGCAGCATG 
GACACCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003354.2
CDS	80..2971
Translation	MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN

Target ORF information:

RefSeq Version	NM_003363.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 4 (USP4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003363.3

ORF Insert Sequence:

ATGGCGGAAG GTGGAGGCTG CCGTGAGCGA CCGGATGCGG AGACTCAGAA GTCCGAGCTT 
GGACCCTTAA TGAGGACCAC ACTCCAACGC GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTTGACAGCT GGGACATGTA CAATGTGGGT 
GAACATAACC TATTTCCTGG CCCAATAGAC AACTCTGGGC TATTTTCAGA TCCTGAGAGT 
CAGACCTTGA AAGAACACTT AATTGATGAA TTGGACTATG TATTGGTCCC TACCGAGGCG 
TGGAATAAAC TACTAAACTG GTACGGCTGT GTAGAAGGCC AGCAACCCAT CGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTCG AGGTGTATTT GCTGGAACTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTGAGTT GCCATTTCAG CAAGGCAGAC 
ACCATTGCAA CCATCGAGAA AGAGATGCGG AAGCTATTCA ACATCCCTGC GGAGCGTGAA 
ACACGGCTCT GGAACAAATA CATGAGCAAC ACCTACGAGC AGTTGAGCAA GCTAGACAAC 
ACTGTCCAGG ATGCTGGGCT ATACCAGGGT CAGGTGCTAG TAATTGAGCC TCAAAATGAA 
GATGGCACAT GGCCCAGGCA GACCTTGCAG TCAAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAG CACCTGTGGG ATGCACAGTT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTTT CTGCTTCGTA TAATTGTCAG GAGCCACCAT CCTCTCATAT ACAACCTGGG 
CTCTGTGGAC TTGGAAACCT GGGAAACACC TGCTTCATGA ACTCCGCTTT GCAGTGTTTG 
AGCAACACTG CACCACTGAC TGACTACTTT CTCAAAGATG AGTATGAAGC CGAAATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAA ATTGCAGAAG CCTATGCTGA ACTCATTAAG 
CAGATGTGGT CTGGAAGGGA CGCCCATGTG GCACCTCGCA TGTTCAAAAC TCAAGTAGGA 
CGTTTTGCTC CTCAATTTTC TGGCTACCAG CAACAAGATT CTCAGGAGCT GCTGGCCTTT 
CTTCTAGATG GATTGCATGA AGATCTGAAC CGGGTAAAGA AAAAGCCCTA CTTGGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCACAGG 
TTGAGGAATG ATTCTGTGAT TGTGGATACT TTCCATGGCC TCTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACGCTGCCA 
CTGCCCTTGA AGAAAGATCG AGTTATGGAG GTTTTCCTGG TTCCTGCTGA CCCTCACTGC 
AGACCTACTC AGTACCGTGT GACTGTGCCG CTGATGGGGG CTGTGTCCGA CCTGTGCGAG 
GCTCTCTCCA GGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCGCAGA TGTGTATAAT 
CACCGATTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTCG TGTACGAGGT CTGCAGCACT TCCGTGGATG GCTCGGAATG TGTCACGCTT 
CCAGTCTACT TCAGGGAGAG GAAGTCCAGG CCATCAAGCA CTTCCTCCGC ATCAGCGCTA 
TATGGGCAGC CACTATTGCT TTCTGTCCCC AAGCACAAGT TAACCCTTGA GTCTTTGTAC 
CAGGCTGTTT GTGATCGTAT CAGCCGCTAT GTGAAACAGC CTTTACCTGA TGAGTTTGGC 
AGCTCACCCT TGGAGCCAGG GGCCTGCAAT GGCTCCAGGA ACAGCTGTGA AGGAGAAGAT 
GAGGAAGAAA TGGAGCATCA GGAAGAAGGC AAAGAGCAGC TTTCAGAAAC AGAAGGCAGT 
GGGGAAGATG AGCCAGGAAA TGACCCCAGT GAGACCACCC AAAAGAAGAT CAAAGGCCAG 
CCCTGCCCAA AAAGGCTTTT TACCTTCAGT CTTGTGAACT CCTATGGAAC AGCTGACATA 
AATTCACTTG CAGCTGATGG AAAACTACTT AAACTCAACT CTCGATCTAC ACTGGCCATG 
GATTGGGACA GTGAAACTCG GAGACTTTAC TATGATGAGC AAGAATCTGA GGCCTACGAG 
AAGCATGTGA GCATGTTGCA GCCTCAGAAG AAGAAGAAGA CCACAGTGGC CCTGAGAGAC 
TGCATCGAGC TCTTCACCAC CATGGAGACC CTTGGGGAGC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCATCAACA GGCCACAAAA AAGTTTGACC TATGGTCCTT GCCCAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AACAGATACT GGAGGGATAA GCTCGACACA 
GTCGTAGAAT TCCCAATCAG AGGGCTGAAC ATGTCCGAGT TTGTCTGTAA CCTGTCAGCA 
AGGCCTTATG TGTACGACCT CATTGCCGTG TCCAATCATT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATATGCGAA GAACAAACTG AATGGTAAAT GGTATTACTT TGATGATAGC 
AACGTGTCCC TGGCCTCTGA GGATCAGATA GTGACTAAAG CAGCTTATGT GCTATTTTAC 
CAACGTCGAG ATGATGAATT TTATAAGACA CCTTCACTTA GCAGTTCTGG TTCCTCTGAT 
GGAGGGACAC GACCAAGCAG CTCTCAGCAG GGCTTTGGGG ATGATGAGGC TTGCAGCATG 
GACACCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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USP4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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