UNG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UNG
Entrez Gene ID	7374
Full Name	uracil DNA glycosylase
Synonyms	DGU,HIGM4,HIGM5,UDG,UNG1,UNG15,UNG2
General protein information	Preferred Names uracil DNA glycosylase Names uracil-DNA glycosylase uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.11
Summary	This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010].
Disorder MIM:	191525
Disorder Html:	Immunodeficiency with hyper IgM, type 5, 608106 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_080911.2	NP_550433.1	uracil-DNA glycosylase isoform UNG2
NM_080911.3	NP_550433.1	uracil-DNA glycosylase isoform UNG2
NM_003362.3	NP_003353.1	uracil-DNA glycosylase isoform UNG1 precursor
NM_003362.4	NP_003353.1	uracil-DNA glycosylase isoform UNG1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UNG	NP_001233452.1
Mus musculus (house mouse)	Ung	NP_001035781.1
Neurospora crassa	NCU07482	XP_965029.1
Xenopus tropicalis (tropical clawed frog)	ung	NP_001016580.1
Caenorhabditis elegans (roundworm)	ung-1	NP_499560.1
Eremothecium gossypii	AGOS_AER327C	NP_985183.1
	UNG	XP_001104341.1
Arabidopsis thaliana (thale cress)	UNG	NP_188493.1
Oryza sativa (rice)	Os04g0673400	NP_001054232.1
Homo sapiens (human)	UNG	NP_550433.1
Canis lupus familiaris (dog)	UNG	XP_543441.2
Bos taurus (cattle)	UNG	NP_001068981.1
Rattus norvegicus (Norway rat)	Ung	NP_001013142.1
Gallus gallus (chicken)	UNG	NP_989520.1
Saccharomyces cerevisiae (baker's yeast)	UNG1	NP_013691.1
Kluyveromyces lactis	KLLA0B01265g	XP_451587.1
Danio rerio (zebrafish)	unga	NP_957268.1
Schizosaccharomyces pombe (fission yeast)	ung1	NP_587889.1
Magnaporthe oryzae (rice blast fungus)	MGG_03632	XP_003716258.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear and mitochondrial splice forms of human uracil-DNA glycosylase contain a complex nuclear localisation signal and a strong classical mitochondrial localisation signal, respectively.
Otterlei M, Haug T, Nagelhus TA, Slupphaug G, Lindmo T, Krokan HE
Nucleic acids research26(20)4611-7(1998 Oct)

Regulation of expression of nuclear and mitochondrial forms of human uracil-DNA glycosylase.
Haug T, Skorpen F, Aas PA, Malm V, Skjelbred C, Krokan HE
Nucleic acids research26(6)1449-57(1998 Mar)

Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene.
Nilsen H, Otterlei M, Haug T, Solum K, Nagelhus TA, Skorpen F, Krokan HE
Nucleic acids research25(4)750-5(1997 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Characterization of the ability of hUNG2 to translocate on DNA.
Title: Measurement of nanoscale DNA translocation by uracil DNA glycosylase in human cells.

Although the N-terminal domain has minimal effects on DNA binding and uracil excision kinetics, this study reports that this domain enhances the ability of human UNG2 to translocate on DNA chains as compared to the catalytic domain alone.
Title: Disordered N-Terminal Domain of Human Uracil DNA Glycosylase (hUNG2) Enhances DNA Translocation.

Data indicate that DNA glycosylases MYH, UNG2, MPG, NTH1, NEIL1, 2 and 3 on nascent DNA.
Title: Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication.

Results suggest that the UNG2 N-terminus may serve as a flexible scaffold to tether PCNA and RPA at the replication fork, and that post-translational modifications on the UNG2 N-terminus disrupt formation of the PCNA-UNG2-RPA protein complex.
Title: Investigation of N-Terminal Phospho-Regulation of?Uracil DNA Glycosylase Using Protein Semisynthesis.

Over-expression of uracil DNA glycosylase 2 in HepG2 protect cells against oxidative stress damage.
Title: [Over-expression of uracil DNA glycosylase 2 (UNG2) enhances the resistance to oxidative damage in HepG2 cells].

The following UNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21730	NM_003362.4 Latest version!	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu27000	NM_080911.3 Latest version!	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu27000	NM_080911.2	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu21730	NM_003362.3	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UNG ( NM_003362.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003353.1 Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21730
Clone ID Related Accession (Same CDS sequence)	NM_003362.3 , NM_003362.4
Accession Version	NM_003362.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	uracil-DNA glycosylase isoform UNG1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB045515.1, BM928006.1, AC007637.9 and BU622689.1. On Nov 3, 2010 this sequence version replaced NM_003362.2. Transcript Variant: This variant (1) uses the downstream promoter and a full-length exon 1B. It encodes the UNG1 isoform, which includes a mitochondrial targeting sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.573367.1, SRR1803611.228005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGTCT TCTGCCTTGG GCCGTGGGGG TTGGGCCGGA AGCTGCGGAC GCCTGGGAAG 
GGGCCGCTGC AGCTCTTGAG CCGCCTCTGC GGGGACCACT TGCAGGCCAT CCCAGCCAAG 
AAGGCCCCGG CTGGGCAGGA GGAGCCTGGG ACGCCGCCCT CCTCGCCGCT GAGTGCCGAG 
CAGTTGGACC GGATCCAGAG GAACAAGGCC GCGGCCCTGC TCAGACTCGC GGCCCGCAAC 
GTGCCCGTGG GCTTTGGAGA GAGCTGGAAG AAGCACCTCA GCGGGGAGTT CGGGAAACCG 
TATTTTATCA AGCTAATGGG ATTTGTTGCA GAAGAAAGAA AGCATTACAC TGTTTATCCA 
CCCCCACACC AAGTCTTCAC CTGGACCCAG ATGTGTGACA TAAAAGATGT GAAGGTTGTC 
ATCCTGGGAC AGGATCCATA TCATGGACCT AATCAAGCTC ACGGGCTCTG CTTTAGTGTT 
CAAAGGCCTG TTCCGCCTCC GCCCAGTTTG GAGAACATTT ATAAAGAGTT GTCTACAGAC 
ATAGAGGATT TTGTTCATCC TGGCCATGGA GATTTATCTG GGTGGGCCAA GCAAGGTGTT 
CTCCTTCTCA ACGCTGTCCT CACGGTTCGT GCCCATCAAG CCAACTCTCA TAAGGAGCGA 
GGCTGGGAGC AGTTCACTGA TGCAGTTGTG TCCTGGCTAA ATCAGAACTC GAATGGCCTT 
GTTTTCTTGC TCTGGGGCTC TTATGCTCAG AAGAAGGGCA GTGCCATTGA TAGGAAGCGG 
CACCATGTAC TACAGACGGC TCATCCCTCC CCTTTGTCAG TGTATAGAGG GTTCTTTGGA 
TGTAGACACT TTTCAAAGAC CAATGAGCTG CTGCAGAAGT CTGGCAAGAA GCCCATTGAC 
TGGAAGGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003353.1
CDS	210..1124
Translation	MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

Target ORF information:

RefSeq Version	NM_003362.3
Organism	Homo sapiens(human)
Definition	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003362.3

ORF Insert Sequence:

ATGGGCGTCT TCTGCCTTGG GCCGTGGGGG TTGGGCCGGA AGCTGCGGAC GCCTGGGAAG 
GGGCCGCTGC AGCTCTTGAG CCGCCTCTGC GGGGACCACT TGCAGGCCAT CCCAGCCAAG 
AAGGCCCCGG CTGGGCAGGA GGAGCCTGGG ACGCCGCCCT CCTCGCCGCT GAGTGCCGAG 
CAGTTGGACC GGATCCAGAG GAACAAGGCC GCGGCCCTGC TCAGACTCGC GGCCCGCAAC 
GTGCCCGTGG GCTTTGGAGA GAGCTGGAAG AAGCACCTCA GCGGGGAGTT CGGGAAACCG 
TATTTTATCA AGCTAATGGG ATTTGTTGCA GAAGAAAGAA AGCATTACAC TGTTTATCCA 
CCCCCACACC AAGTCTTCAC CTGGACCCAG ATGTGTGACA TAAAAGATGT GAAGGTTGTC 
ATCCTGGGAC AGGATCCATA TCATGGACCT AATCAAGCTC ACGGGCTCTG CTTTAGTGTT 
CAAAGGCCTG TTCCGCCTCC GCCCAGTTTG GAGAACATTT ATAAAGAGTT GTCTACAGAC 
ATAGAGGATT TTGTTCATCC TGGCCATGGA GATTTATCTG GGTGGGCCAA GCAAGGTGTT 
CTCCTTCTCA ACGCTGTCCT CACGGTTCGT GCCCATCAAG CCAACTCTCA TAAGGAGCGA 
GGCTGGGAGC AGTTCACTGA TGCAGTTGTG TCCTGGCTAA ATCAGAACTC GAATGGCCTT 
GTTTTCTTGC TCTGGGGCTC TTATGCTCAG AAGAAGGGCA GTGCCATTGA TAGGAAGCGG 
CACCATGTAC TACAGACGGC TCATCCCTCC CCTTTGTCAG TGTATAGAGG GTTCTTTGGA 
TGTAGACACT TTTCAAAGAC CAATGAGCTG CTGCAGAAGT CTGGCAAGAA GCCCATTGAC 
TGGAAGGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNG ( NM_003362.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003353.1 Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21730
Clone ID Related Accession (Same CDS sequence)	NM_003362.3 , NM_003362.4
Accession Version	NM_003362.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	uracil-DNA glycosylase isoform UNG1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB045515.1, BM928006.1, AC007637.9 and BU622689.1. On Jun 2, 2019 this sequence version replaced NM_003362.3. Transcript Variant: This variant (1) uses the downstream promoter and a full-length exon 1B. It encodes the UNG1 isoform, which includes a mitochondrial targeting sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15653.1, BC050634.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGTCT TCTGCCTTGG GCCGTGGGGG TTGGGCCGGA AGCTGCGGAC GCCTGGGAAG 
GGGCCGCTGC AGCTCTTGAG CCGCCTCTGC GGGGACCACT TGCAGGCCAT CCCAGCCAAG 
AAGGCCCCGG CTGGGCAGGA GGAGCCTGGG ACGCCGCCCT CCTCGCCGCT GAGTGCCGAG 
CAGTTGGACC GGATCCAGAG GAACAAGGCC GCGGCCCTGC TCAGACTCGC GGCCCGCAAC 
GTGCCCGTGG GCTTTGGAGA GAGCTGGAAG AAGCACCTCA GCGGGGAGTT CGGGAAACCG 
TATTTTATCA AGCTAATGGG ATTTGTTGCA GAAGAAAGAA AGCATTACAC TGTTTATCCA 
CCCCCACACC AAGTCTTCAC CTGGACCCAG ATGTGTGACA TAAAAGATGT GAAGGTTGTC 
ATCCTGGGAC AGGATCCATA TCATGGACCT AATCAAGCTC ACGGGCTCTG CTTTAGTGTT 
CAAAGGCCTG TTCCGCCTCC GCCCAGTTTG GAGAACATTT ATAAAGAGTT GTCTACAGAC 
ATAGAGGATT TTGTTCATCC TGGCCATGGA GATTTATCTG GGTGGGCCAA GCAAGGTGTT 
CTCCTTCTCA ACGCTGTCCT CACGGTTCGT GCCCATCAAG CCAACTCTCA TAAGGAGCGA 
GGCTGGGAGC AGTTCACTGA TGCAGTTGTG TCCTGGCTAA ATCAGAACTC GAATGGCCTT 
GTTTTCTTGC TCTGGGGCTC TTATGCTCAG AAGAAGGGCA GTGCCATTGA TAGGAAGCGG 
CACCATGTAC TACAGACGGC TCATCCCTCC CCTTTGTCAG TGTATAGAGG GTTCTTTGGA 
TGTAGACACT TTTCAAAGAC CAATGAGCTG CTGCAGAAGT CTGGCAAGAA GCCCATTGAC 
TGGAAGGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003353.1
CDS	156..1070
Translation	MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

Target ORF information:

RefSeq Version	NM_003362.4
Organism	Homo sapiens(human)
Definition	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003362.4

ORF Insert Sequence:

ATGGGCGTCT TCTGCCTTGG GCCGTGGGGG TTGGGCCGGA AGCTGCGGAC GCCTGGGAAG 
GGGCCGCTGC AGCTCTTGAG CCGCCTCTGC GGGGACCACT TGCAGGCCAT CCCAGCCAAG 
AAGGCCCCGG CTGGGCAGGA GGAGCCTGGG ACGCCGCCCT CCTCGCCGCT GAGTGCCGAG 
CAGTTGGACC GGATCCAGAG GAACAAGGCC GCGGCCCTGC TCAGACTCGC GGCCCGCAAC 
GTGCCCGTGG GCTTTGGAGA GAGCTGGAAG AAGCACCTCA GCGGGGAGTT CGGGAAACCG 
TATTTTATCA AGCTAATGGG ATTTGTTGCA GAAGAAAGAA AGCATTACAC TGTTTATCCA 
CCCCCACACC AAGTCTTCAC CTGGACCCAG ATGTGTGACA TAAAAGATGT GAAGGTTGTC 
ATCCTGGGAC AGGATCCATA TCATGGACCT AATCAAGCTC ACGGGCTCTG CTTTAGTGTT 
CAAAGGCCTG TTCCGCCTCC GCCCAGTTTG GAGAACATTT ATAAAGAGTT GTCTACAGAC 
ATAGAGGATT TTGTTCATCC TGGCCATGGA GATTTATCTG GGTGGGCCAA GCAAGGTGTT 
CTCCTTCTCA ACGCTGTCCT CACGGTTCGT GCCCATCAAG CCAACTCTCA TAAGGAGCGA 
GGCTGGGAGC AGTTCACTGA TGCAGTTGTG TCCTGGCTAA ATCAGAACTC GAATGGCCTT 
GTTTTCTTGC TCTGGGGCTC TTATGCTCAG AAGAAGGGCA GTGCCATTGA TAGGAAGCGG 
CACCATGTAC TACAGACGGC TCATCCCTCC CCTTTGTCAG TGTATAGAGG GTTCTTTGGA 
TGTAGACACT TTTCAAAGAC CAATGAGCTG CTGCAGAAGT CTGGCAAGAA GCCCATTGAC 
TGGAAGGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNG ( NM_080911.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_550433.1 Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27000
Clone ID Related Accession (Same CDS sequence)	NM_080911.2 , NM_080911.3
Accession Version	NM_080911.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	uracil-DNA glycosylase isoform UNG2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ950839.1, Y09008.1 and BU622689.1. On Nov 3, 2010 this sequence version replaced NM_080911.1. Transcript Variant: This variant (2) uses an upstream promoter and an alternate splice site for exon 1B when compared to variant 1. It encodes the nuclear UNG2 isoform, which has a different N-terminal sequence than the UNG1 isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.158175.1, Y09008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCGGCC AGAAGACGCT CTACTCCTTT TTCTCCCCCA GCCCCGCCAG GAAGCGACAC 
GCCCCCAGCC CCGAGCCGGC CGTCCAGGGG ACCGGCGTGG CTGGGGTGCC TGAGGAAAGC 
GGAGATGCGG CGGCCATCCC AGCCAAGAAG GCCCCGGCTG GGCAGGAGGA GCCTGGGACG 
CCGCCCTCCT CGCCGCTGAG TGCCGAGCAG TTGGACCGGA TCCAGAGGAA CAAGGCCGCG 
GCCCTGCTCA GACTCGCGGC CCGCAACGTG CCCGTGGGCT TTGGAGAGAG CTGGAAGAAG 
CACCTCAGCG GGGAGTTCGG GAAACCGTAT TTTATCAAGC TAATGGGATT TGTTGCAGAA 
GAAAGAAAGC ATTACACTGT TTATCCACCC CCACACCAAG TCTTCACCTG GACCCAGATG 
TGTGACATAA AAGATGTGAA GGTTGTCATC CTGGGACAGG ATCCATATCA TGGACCTAAT 
CAAGCTCACG GGCTCTGCTT TAGTGTTCAA AGGCCTGTTC CGCCTCCGCC CAGTTTGGAG 
AACATTTATA AAGAGTTGTC TACAGACATA GAGGATTTTG TTCATCCTGG CCATGGAGAT 
TTATCTGGGT GGGCCAAGCA AGGTGTTCTC CTTCTCAACG CTGTCCTCAC GGTTCGTGCC 
CATCAAGCCA ACTCTCATAA GGAGCGAGGC TGGGAGCAGT TCACTGATGC AGTTGTGTCC 
TGGCTAAATC AGAACTCGAA TGGCCTTGTT TTCTTGCTCT GGGGCTCTTA TGCTCAGAAG 
AAGGGCAGTG CCATTGATAG GAAGCGGCAC CATGTACTAC AGACGGCTCA TCCCTCCCCT 
TTGTCAGTGT ATAGAGGGTT CTTTGGATGT AGACACTTTT CAAAGACCAA TGAGCTGCTG 
CAGAAGTCTG GCAAGAAGCC CATTGACTGG AAGGAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_550433.1
CDS	87..1028
Translation	MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

Target ORF information:

RefSeq Version	NM_080911.2
Organism	Homo sapiens(human)
Definition	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080911.2

ORF Insert Sequence:

ATGATCGGCC AGAAGACGCT CTACTCCTTT TTCTCCCCCA GCCCCGCCAG GAAGCGACAC 
GCCCCCAGCC CCGAGCCGGC CGTCCAGGGG ACCGGCGTGG CTGGGGTGCC TGAGGAAAGC 
GGAGATGCGG CGGCCATCCC AGCCAAGAAG GCCCCGGCTG GGCAGGAGGA GCCTGGGACG 
CCGCCCTCCT CGCCGCTGAG TGCCGAGCAG TTGGACCGGA TCCAGAGGAA CAAGGCCGCG 
GCCCTGCTCA GACTCGCGGC CCGCAACGTG CCCGTGGGCT TTGGAGAGAG CTGGAAGAAG 
CACCTCAGCG GGGAGTTCGG GAAACCGTAT TTTATCAAGC TAATGGGATT TGTTGCAGAA 
GAAAGAAAGC ATTACACTGT TTATCCACCC CCACACCAAG TCTTCACCTG GACCCAGATG 
TGTGACATAA AAGATGTGAA GGTTGTCATC CTGGGACAGG ATCCATATCA TGGACCTAAT 
CAAGCTCACG GGCTCTGCTT TAGTGTTCAA AGGCCTGTTC CGCCTCCGCC CAGTTTGGAG 
AACATTTATA AAGAGTTGTC TACAGACATA GAGGATTTTG TTCATCCTGG CCATGGAGAT 
TTATCTGGGT GGGCCAAGCA AGGTGTTCTC CTTCTCAACG CTGTCCTCAC GGTTCGTGCC 
CATCAAGCCA ACTCTCATAA GGAGCGAGGC TGGGAGCAGT TCACTGATGC AGTTGTGTCC 
TGGCTAAATC AGAACTCGAA TGGCCTTGTT TTCTTGCTCT GGGGCTCTTA TGCTCAGAAG 
AAGGGCAGTG CCATTGATAG GAAGCGGCAC CATGTACTAC AGACGGCTCA TCCCTCCCCT 
TTGTCAGTGT ATAGAGGGTT CTTTGGATGT AGACACTTTT CAAAGACCAA TGAGCTGCTG 
CAGAAGTCTG GCAAGAAGCC CATTGACTGG AAGGAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNG ( NM_080911.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_550433.1 Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27000
Clone ID Related Accession (Same CDS sequence)	NM_080911.2 , NM_080911.3
Accession Version	NM_080911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	uracil-DNA glycosylase isoform UNG2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ950839.1, Y09008.1 and BU622689.1. On Jun 2, 2019 this sequence version replaced NM_080911.2. Transcript Variant: This variant (2) uses an upstream promoter and an alternate splice site for exon 1B when compared to variant 1. It encodes the nuclear UNG2 isoform, which has a different N-terminal sequence than the UNG1 isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y09008.1, DRR138512.105435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCGGCC AGAAGACGCT CTACTCCTTT TTCTCCCCCA GCCCCGCCAG GAAGCGACAC 
GCCCCCAGCC CCGAGCCGGC CGTCCAGGGG ACCGGCGTGG CTGGGGTGCC TGAGGAAAGC 
GGAGATGCGG CGGCCATCCC AGCCAAGAAG GCCCCGGCTG GGCAGGAGGA GCCTGGGACG 
CCGCCCTCCT CGCCGCTGAG TGCCGAGCAG TTGGACCGGA TCCAGAGGAA CAAGGCCGCG 
GCCCTGCTCA GACTCGCGGC CCGCAACGTG CCCGTGGGCT TTGGAGAGAG CTGGAAGAAG 
CACCTCAGCG GGGAGTTCGG GAAACCGTAT TTTATCAAGC TAATGGGATT TGTTGCAGAA 
GAAAGAAAGC ATTACACTGT TTATCCACCC CCACACCAAG TCTTCACCTG GACCCAGATG 
TGTGACATAA AAGATGTGAA GGTTGTCATC CTGGGACAGG ATCCATATCA TGGACCTAAT 
CAAGCTCACG GGCTCTGCTT TAGTGTTCAA AGGCCTGTTC CGCCTCCGCC CAGTTTGGAG 
AACATTTATA AAGAGTTGTC TACAGACATA GAGGATTTTG TTCATCCTGG CCATGGAGAT 
TTATCTGGGT GGGCCAAGCA AGGTGTTCTC CTTCTCAACG CTGTCCTCAC GGTTCGTGCC 
CATCAAGCCA ACTCTCATAA GGAGCGAGGC TGGGAGCAGT TCACTGATGC AGTTGTGTCC 
TGGCTAAATC AGAACTCGAA TGGCCTTGTT TTCTTGCTCT GGGGCTCTTA TGCTCAGAAG 
AAGGGCAGTG CCATTGATAG GAAGCGGCAC CATGTACTAC AGACGGCTCA TCCCTCCCCT 
TTGTCAGTGT ATAGAGGGTT CTTTGGATGT AGACACTTTT CAAAGACCAA TGAGCTGCTG 
CAGAAGTCTG GCAAGAAGCC CATTGACTGG AAGGAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_550433.1
CDS	84..1025
Translation	MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

Target ORF information:

RefSeq Version	NM_080911.3
Organism	Homo sapiens(human)
Definition	Homo sapiens uracil DNA glycosylase (UNG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080911.3

ORF Insert Sequence:

ATGATCGGCC AGAAGACGCT CTACTCCTTT TTCTCCCCCA GCCCCGCCAG GAAGCGACAC 
GCCCCCAGCC CCGAGCCGGC CGTCCAGGGG ACCGGCGTGG CTGGGGTGCC TGAGGAAAGC 
GGAGATGCGG CGGCCATCCC AGCCAAGAAG GCCCCGGCTG GGCAGGAGGA GCCTGGGACG 
CCGCCCTCCT CGCCGCTGAG TGCCGAGCAG TTGGACCGGA TCCAGAGGAA CAAGGCCGCG 
GCCCTGCTCA GACTCGCGGC CCGCAACGTG CCCGTGGGCT TTGGAGAGAG CTGGAAGAAG 
CACCTCAGCG GGGAGTTCGG GAAACCGTAT TTTATCAAGC TAATGGGATT TGTTGCAGAA 
GAAAGAAAGC ATTACACTGT TTATCCACCC CCACACCAAG TCTTCACCTG GACCCAGATG 
TGTGACATAA AAGATGTGAA GGTTGTCATC CTGGGACAGG ATCCATATCA TGGACCTAAT 
CAAGCTCACG GGCTCTGCTT TAGTGTTCAA AGGCCTGTTC CGCCTCCGCC CAGTTTGGAG 
AACATTTATA AAGAGTTGTC TACAGACATA GAGGATTTTG TTCATCCTGG CCATGGAGAT 
TTATCTGGGT GGGCCAAGCA AGGTGTTCTC CTTCTCAACG CTGTCCTCAC GGTTCGTGCC 
CATCAAGCCA ACTCTCATAA GGAGCGAGGC TGGGAGCAGT TCACTGATGC AGTTGTGTCC 
TGGCTAAATC AGAACTCGAA TGGCCTTGTT TTCTTGCTCT GGGGCTCTTA TGCTCAGAAG 
AAGGGCAGTG CCATTGATAG GAAGCGGCAC CATGTACTAC AGACGGCTCA TCCCTCCCCT 
TTGTCAGTGT ATAGAGGGTT CTTTGGATGT AGACACTTTT CAAAGACCAA TGAGCTGCTG 
CAGAAGTCTG GCAAGAAGCC CATTGACTGG AAGGAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear and mitochondrial splice forms of human uracil-DNA glycosylase contain a complex nuclear localisation signal and a strong classical mitochondrial localisation signal, respectively.
Nucleic acids research26(20)4611-7(1998 Oct)
Otterlei M,Haug T,Nagelhus TA,Slupphaug G,Lindmo T,Krokan HE

Regulation of expression of nuclear and mitochondrial forms of human uracil-DNA glycosylase.
Nucleic acids research26(6)1449-57(1998 Mar)
Haug T,Skorpen F,Aas PA,Malm V,Skjelbred C,Krokan HE

Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene.
Nucleic acids research25(4)750-5(1997 Feb)
Nilsen H,Otterlei M,Haug T,Solum K,Nagelhus TA,Skorpen F,Krokan HE

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UNG cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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