UBE2V2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2V2
Entrez Gene ID	7336
Full Name	ubiquitin conjugating enzyme E2 V2
Synonyms	DDVIT1,DDVit-1,EDAF-1,EDPF-1,EDPF1,MMS2,UEV-2,UEV2
General protein information	Preferred Names ubiquitin conjugating enzyme E2 V2 Names ubiquitin-conjugating enzyme E2 variant 2 1 alpha,25-dihydroxyvitamin D3-inducible DDVit 1 MMS2 homolog enterocyte differentiation promoting factor enterocyte differentiation-associated factor 1 enterocyte differentiation-associated factor EDAF-1 enterocyte differentiation-promoting factor 1 methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of vitamin D3-inducible protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q11.21
Summary	Ubiquitin-conjugating enzyme E2 variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes. [provided by RefSeq, Jul 2008].
Disorder MIM:	603001

mRNA and Protein(s)

mRNA	Protein	Name
XM_011517583.2	XP_011515885.1	ubiquitin-conjugating enzyme E2 variant 2 isoform X1
XM_017013808.1	XP_016869297.1	ubiquitin-conjugating enzyme E2 variant 2 isoform X2
NM_003350.2	NP_003341.1	ubiquitin-conjugating enzyme E2 variant 2
XM_005251300.2	XP_005251357.1	ubiquitin-conjugating enzyme E2 variant 2 isoform X3
NM_003350.3	NP_003341.1	ubiquitin-conjugating enzyme E2 variant 2
XM_011517583.3	XP_011515885.1	ubiquitin-conjugating enzyme E2 variant 2 isoform X1
XM_017013808.2	XP_016869297.1	ubiquitin-conjugating enzyme E2 variant 2 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	UBE2V2	NP_001026189.1
Saccharomyces cerevisiae (baker's yeast)	MMS2	NP_011428.1
Kluyveromyces lactis	KLLA0E19119g	XP_454816.1
Eremothecium gossypii	AGOS_AFL064W	NP_985484.1
Schizosaccharomyces pombe (fission yeast)	mms2	NP_588162.1
Neurospora crassa	NCU10477	XP_001728241.1
Bos taurus (cattle)	UBE2V2	NP_001029214.1
Danio rerio (zebrafish)	ube2v2	NP_998680.1
Oryza sativa (rice)	Os09g0297100	NP_001062804.1
	UBE2V2	XP_001104640.1
Mus musculus (house mouse)	Ube2v2	NP_076074.2
Rattus norvegicus (Norway rat)	Ube2v2	NP_898875.1
Homo sapiens (human)	UBE2V2	NP_003341.1
Canis lupus familiaris (dog)	UBE2V2	NP_001192044.1
Magnaporthe oryzae (rice blast fungus)	MGG_07914	XP_003713156.1
Arabidopsis thaliana (thale cress)	MMZ3	NP_850259.1
Arabidopsis thaliana (thale cress)	UEV1D-4	NP_850684.1
Xenopus tropicalis (tropical clawed frog)	ube2v2	NP_001016276.1

Gene Ontology
Bibliography

Related articles in PubMed

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.
Chai F, Li HY, Wang W, Zhu XJ, Li Y, Wang S, Guo L, Zhang LK, Xiao G
Biochimica et biophysica acta1854(8)967-78(2015 Aug)

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.
Branigan E, Plechanovov? A, Jaffray EG, Naismith JH, Hay RT
Nature structural & molecular biology22(8)597-602(2015 Aug)

[Roles of hMMS2 gene in reversing the oxaliplatin tolerance of human colon carcinoma cells].
Zhang L, Sui Y, Wang T, Li L, Li Y, Jin C, Xu F
Yi chuan = Hereditas36(4)346-53(2014 Apr)

The tomato Fni3 lysine-63-specific ubiquitin-conjugating enzyme and suv ubiquitin E2 variant positively regulate plant immunity.
Mural RV, Liu Y, Rosebrock TR, Brady JJ, Hamera S, Connor RA, Martin GB, Zeng L
The Plant cell25(9)3615-31(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional analysis of selected regulated proteins revealed that knockdown of HNRPD, PHB2 and UB2V2 can increase HCMV replication, while knockdown of A4 and KSRP resulted in decreased HCMV replication.
Title: Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.

Data suggest that ubiquitin-conjugating enzyme E2 variant 2 (Ube2V2) and ring finger protein 4 (RNF4) together induce an active conformation of the ubiquitin-conjugating enzyme Ubc13-ubiquitin (Ubc13~Ub) thioester.
Title: Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.

suppression of hMMS2 reverses L-OHP tolerance in differentiated human colorectal carcinoma cells by promoting apoptosis
Title: [Roles of hMMS2 gene in reversing the oxaliplatin tolerance of human colon carcinoma cells].

Data show RING finger (RNF) E3 ubiquitin ligase RNF8 dimerizes and binds to E2 ubiquitin-conjugating complex Ubc13/Mms2 with formation of Lys-63 ubiquitin chains, whereas the RNF168 RING domain is a monomer and does not catalyze Lys-6 ubiquitylation.
Title: Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation.

the crystal structure of human OTUB1 in complex with human UBC13 and MMS2
Title: Molecular basis of Lys-63-linked polyubiquitination inhibition by the interaction between human deubiquitinating enzyme OTUB1 and ubiquitin-conjugating enzyme UBC13.

The following UBE2V2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2V2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03303	NM_003350.3 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu42297	XM_005251300.2 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu61214	XM_011517583.3 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu98191	XM_017013808.2 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu03303	NM_003350.2	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu61214	XM_011517583.2	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu98191	XM_017013808.1	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2V2 ( NM_003350.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003341.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.

Price & Availability↑

CloneID	OHu03303
Clone ID Related Accession (Same CDS sequence)	NM_003350.2 , NM_003350.3
Accession Version	NM_003350.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF049140.1. On Jan 4, 2001 this sequence version replaced NM_003350.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF049140.1, SRR1660803.108140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCT CCACAGGAGT TAAAGTTCCT CGTAATTTTC GCTTGTTGGA AGAACTTGAA 
GAAGGACAAA AAGGAGTAGG CGACGGTACA GTTAGCTGGG GCCTTGAAGA TGATGAAGAT 
ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003341.1
CDS	22..459
Translation	MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	NM_003350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003350.2

ORF Insert Sequence:

ATGGCGGTCT CCACAGGAGT TAAAGTTCCT CGTAATTTTC GCTTGTTGGA AGAACTTGAA 
GAAGGACAAA AAGGAGTAGG CGACGGTACA GTTAGCTGGG GCCTTGAAGA TGATGAAGAT 
ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( NM_003350.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003341.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.

Price & Availability↑

CloneID	OHu03303
Clone ID Related Accession (Same CDS sequence)	NM_003350.2 , NM_003350.3
Accession Version	NM_003350.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021236.10. On Aug 17, 2018 this sequence version replaced NM_003350.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.427974.1, SRR1660805.19159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000523111.7/ ENSP00000428209.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCT CCACAGGAGT TAAAGTTCCT CGTAATTTTC GCTTGTTGGA AGAACTTGAA 
GAAGGACAAA AAGGAGTAGG CGACGGTACA GTTAGCTGGG GCCTTGAAGA TGATGAAGAT 
ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003341.1
CDS	25..462
Translation	MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	NM_003350.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003350.3

ORF Insert Sequence:

ATGGCGGTCT CCACAGGAGT TAAAGTTCCT CGTAATTTTC GCTTGTTGGA AGAACTTGAA 
GAAGGACAAA AAGGAGTAGG CGACGGTACA GTTAGCTGGG GCCTTGAAGA TGATGAAGAT 
ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( XM_005251300.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251357.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu42297
Clone ID Related Accession (Same CDS sequence)	XM_005251300.2
Accession Version	XM_005251300.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251357.1
CDS	201..518
Translation	MTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	XM_005251300.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251300.2

ORF Insert Sequence:

ATGACACTTA CAAGGTGGAC AGGCATGATT ATTGGGCCAC CAAGGACAAA TTATGAAAAC 
AGAATATATA GCCTGAAAGT AGAATGTGGA CCTAAATACC CAGAAGCTCC TCCGTCAGTT 
AGATTTGTAA CAAAAATTAA TATGAACGGA ATAAATAATT CCAGTGGGAT GGTGGATGCC 
CGGAGCATAC CAGTGTTAGC AAAATGGCAA AATTCATATA GCATTAAAGT TGTACTTCAA 
GAGCTAAGAC GTCTAATGAT GTCCAAAGAA AATATGAAGC TTCCACAGCC ACCAGAAGGA 
CAAACATACA ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( XM_011517583.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515885.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61214
Clone ID Related Accession (Same CDS sequence)	XM_011517583.2 , XM_011517583.3
Accession Version	XM_011517583.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG GGGCGCACGT AGAAGAAGAA GAGACTGCCG GTGACAAGGA CGTGGAAGAG 
GGCGTTCCCT TTCCTGGGGA ACTTGTCTGG AATAGAGAAG GAGTTAAAGT TCCTCGTAAT 
TTTCGCTTGT TGGAAGAACT TGAAGAAGGA CAAAAAGGAG TAGGCGACGG TACAGTTAGC 
TGGGGCCTTG AAGATGATGA AGATATGACA CTTACAAGGT GGACAGGCAT GATTATTGGG 
CCACCAAGGA CAAATTATGA AAACAGAATA TATAGCCTGA AAGTAGAATG TGGACCTAAA 
TACCCAGAAG CTCCTCCGTC AGTTAGATTT GTAACAAAAA TTAATATGAA CGGAATAAAT 
AATTCCAGTG GGATGGTGGA TGCCCGGAGC ATACCAGTGT TAGCAAAATG GCAAAATTCA 
TATAGCATTA AAGTTGTACT TCAAGAGCTA AGACGTCTAA TGATGTCCAA AGAAAATATG 
AAGCTTCCAC AGCCACCAGA AGGACAAACA TACAACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515885.1
CDS	16..537
Translation	MEVGAHVEEEETAGDKDVEEGVPFPGELVWNREGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	XM_011517583.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517583.2

ORF Insert Sequence:

ATGGAAGTAG GGGCGCACGT AGAAGAAGAA GAGACTGCCG GTGACAAGGA CGTGGAAGAG 
GGCGTTCCCT TTCCTGGGGA ACTTGTCTGG AATAGAGAAG GAGTTAAAGT TCCTCGTAAT 
TTTCGCTTGT TGGAAGAACT TGAAGAAGGA CAAAAAGGAG TAGGCGACGG TACAGTTAGC 
TGGGGCCTTG AAGATGATGA AGATATGACA CTTACAAGGT GGACAGGCAT GATTATTGGG 
CCACCAAGGA CAAATTATGA AAACAGAATA TATAGCCTGA AAGTAGAATG TGGACCTAAA 
TACCCAGAAG CTCCTCCGTC AGTTAGATTT GTAACAAAAA TTAATATGAA CGGAATAAAT 
AATTCCAGTG GGATGGTGGA TGCCCGGAGC ATACCAGTGT TAGCAAAATG GCAAAATTCA 
TATAGCATTA AAGTTGTACT TCAAGAGCTA AGACGTCTAA TGATGTCCAA AGAAAATATG 
AAGCTTCCAC AGCCACCAGA AGGACAAACA TACAACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( XM_011517583.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515885.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61214
Clone ID Related Accession (Same CDS sequence)	XM_011517583.2 , XM_011517583.3
Accession Version	XM_011517583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011517583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG GGGCGCACGT AGAAGAAGAA GAGACTGCCG GTGACAAGGA CGTGGAAGAG 
GGCGTTCCCT TTCCTGGGGA ACTTGTCTGG AATAGAGAAG GAGTTAAAGT TCCTCGTAAT 
TTTCGCTTGT TGGAAGAACT TGAAGAAGGA CAAAAAGGAG TAGGCGACGG TACAGTTAGC 
TGGGGCCTTG AAGATGATGA AGATATGACA CTTACAAGGT GGACAGGCAT GATTATTGGG 
CCACCAAGGA CAAATTATGA AAACAGAATA TATAGCCTGA AAGTAGAATG TGGACCTAAA 
TACCCAGAAG CTCCTCCGTC AGTTAGATTT GTAACAAAAA TTAATATGAA CGGAATAAAT 
AATTCCAGTG GGATGGTGGA TGCCCGGAGC ATACCAGTGT TAGCAAAATG GCAAAATTCA 
TATAGCATTA AAGTTGTACT TCAAGAGCTA AGACGTCTAA TGATGTCCAA AGAAAATATG 
AAGCTTCCAC AGCCACCAGA AGGACAAACA TACAACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515885.1
CDS	16..537
Translation	MEVGAHVEEEETAGDKDVEEGVPFPGELVWNREGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	XM_011517583.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517583.3

ORF Insert Sequence:

ATGGAAGTAG GGGCGCACGT AGAAGAAGAA GAGACTGCCG GTGACAAGGA CGTGGAAGAG 
GGCGTTCCCT TTCCTGGGGA ACTTGTCTGG AATAGAGAAG GAGTTAAAGT TCCTCGTAAT 
TTTCGCTTGT TGGAAGAACT TGAAGAAGGA CAAAAAGGAG TAGGCGACGG TACAGTTAGC 
TGGGGCCTTG AAGATGATGA AGATATGACA CTTACAAGGT GGACAGGCAT GATTATTGGG 
CCACCAAGGA CAAATTATGA AAACAGAATA TATAGCCTGA AAGTAGAATG TGGACCTAAA 
TACCCAGAAG CTCCTCCGTC AGTTAGATTT GTAACAAAAA TTAATATGAA CGGAATAAAT 
AATTCCAGTG GGATGGTGGA TGCCCGGAGC ATACCAGTGT TAGCAAAATG GCAAAATTCA 
TATAGCATTA AAGTTGTACT TCAAGAGCTA AGACGTCTAA TGATGTCCAA AGAAAATATG 
AAGCTTCCAC AGCCACCAGA AGGACAAACA TACAACAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( XM_017013808.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869297.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.

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CloneID	OHu98191
Clone ID Related Accession (Same CDS sequence)	XM_017013808.1 , XM_017013808.2
Accession Version	XM_017013808.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAG TTAAAGTTCC TCGTAATTTT CGCTTGTTGG AAGAACTTGA AGAAGGACAA 
AAAGGAGTAG GCGACGGTAC AGTTAGCTGG GGCCTTGAAG ATGATGAAGA TATGACACTT 
ACAAGGTGGA CAGGCATGAT TATTGGGCCA CCAAGGACAA ATTATGAAAA CAGAATATAT 
AGCCTGAAAG TAGAATGTGG ACCTAAATAC CCAGAAGCTC CTCCGTCAGT TAGATTTGTA 
ACAAAAATTA ATATGAACGG AATAAATAAT TCCAGTGGGA TGGTGGATGC CCGGAGCATA 
CCAGTGTTAG CAAAATGGCA AAATTCATAT AGCATTAAAG TTGTACTTCA AGAGCTAAGA 
CGTCTAATGA TGTCCAAAGA AAATATGAAG CTTCCACAGC CACCAGAAGG ACAAACATAC 
AACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869297.1
CDS	16..444
Translation	MPRVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	XM_017013808.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013808.1

ORF Insert Sequence:

ATGCCAAGAG TTAAAGTTCC TCGTAATTTT CGCTTGTTGG AAGAACTTGA AGAAGGACAA 
AAAGGAGTAG GCGACGGTAC AGTTAGCTGG GGCCTTGAAG ATGATGAAGA TATGACACTT 
ACAAGGTGGA CAGGCATGAT TATTGGGCCA CCAAGGACAA ATTATGAAAA CAGAATATAT 
AGCCTGAAAG TAGAATGTGG ACCTAAATAC CCAGAAGCTC CTCCGTCAGT TAGATTTGTA 
ACAAAAATTA ATATGAACGG AATAAATAAT TCCAGTGGGA TGGTGGATGC CCGGAGCATA 
CCAGTGTTAG CAAAATGGCA AAATTCATAT AGCATTAAAG TTGTACTTCA AGAGCTAAGA 
CGTCTAATGA TGTCCAAAGA AAATATGAAG CTTCCACAGC CACCAGAAGG ACAAACATAC 
AACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2V2 ( XM_017013808.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869297.1 Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu98191
Clone ID Related Accession (Same CDS sequence)	XM_017013808.1 , XM_017013808.2
Accession Version	XM_017013808.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 variant 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017013808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAG TTAAAGTTCC TCGTAATTTT CGCTTGTTGG AAGAACTTGA AGAAGGACAA 
AAAGGAGTAG GCGACGGTAC AGTTAGCTGG GGCCTTGAAG ATGATGAAGA TATGACACTT 
ACAAGGTGGA CAGGCATGAT TATTGGGCCA CCAAGGACAA ATTATGAAAA CAGAATATAT 
AGCCTGAAAG TAGAATGTGG ACCTAAATAC CCAGAAGCTC CTCCGTCAGT TAGATTTGTA 
ACAAAAATTA ATATGAACGG AATAAATAAT TCCAGTGGGA TGGTGGATGC CCGGAGCATA 
CCAGTGTTAG CAAAATGGCA AAATTCATAT AGCATTAAAG TTGTACTTCA AGAGCTAAGA 
CGTCTAATGA TGTCCAAAGA AAATATGAAG CTTCCACAGC CACCAGAAGG ACAAACATAC 
AACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869297.1
CDS	16..444
Translation	MPRVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

Target ORF information:

RefSeq Version	XM_017013808.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013808.2

ORF Insert Sequence:

ATGCCAAGAG TTAAAGTTCC TCGTAATTTT CGCTTGTTGG AAGAACTTGA AGAAGGACAA 
AAAGGAGTAG GCGACGGTAC AGTTAGCTGG GGCCTTGAAG ATGATGAAGA TATGACACTT 
ACAAGGTGGA CAGGCATGAT TATTGGGCCA CCAAGGACAA ATTATGAAAA CAGAATATAT 
AGCCTGAAAG TAGAATGTGG ACCTAAATAC CCAGAAGCTC CTCCGTCAGT TAGATTTGTA 
ACAAAAATTA ATATGAACGG AATAAATAAT TCCAGTGGGA TGGTGGATGC CCGGAGCATA 
CCAGTGTTAG CAAAATGGCA AAATTCATAT AGCATTAAAG TTGTACTTCA AGAGCTAAGA 
CGTCTAATGA TGTCCAAAGA AAATATGAAG CTTCCACAGC CACCAGAAGG ACAAACATAC 
AACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.
Biochimica et biophysica acta1854(8)967-78(2015 Aug)
Chai F,Li HY,Wang W,Zhu XJ,Li Y,Wang S,Guo L,Zhang LK,Xiao G

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.
Nature structural & molecular biology22(8)597-602(2015 Aug)
Branigan E,Plechanovov? A,Jaffray EG,Naismith JH,Hay RT

[Roles of hMMS2 gene in reversing the oxaliplatin tolerance of human colon carcinoma cells].
Yi chuan = Hereditas36(4)346-53(2014 Apr)
Zhang L,Sui Y,Wang T,Li L,Li Y,Jin C,Xu F

The tomato Fni3 lysine-63-specific ubiquitin-conjugating enzyme and suv ubiquitin E2 variant positively regulate plant immunity.
The Plant cell25(9)3615-31(2013 Sep)
Mural RV,Liu Y,Rosebrock TR,Brady JJ,Hamera S,Connor RA,Martin GB,Zeng L

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UBE2V2 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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