UBE2H cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2H
Entrez Gene ID	7328
Full Name	ubiquitin conjugating enzyme E2 H
Synonyms	E2-20K,GID3,UBC8,UBCH,UBCH2
General protein information	Preferred Names ubiquitin conjugating enzyme E2 H Names ubiquitin-conjugating enzyme E2 H (E3-independent) E2 ubiquitin-conjugating enzyme H E2 ubiquitin-conjugating enzyme H GID complex subunit 3, UBC8 homolog ubiquitin carrier protein H ubiquitin conjugating enzyme E2H ubiquitin-conjugating enzyme E2-20K ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) ubiquitin-protein ligase H
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q32.2
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011].
Disorder MIM:	601082

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202498.1	NP_001189427.1	ubiquitin-conjugating enzyme E2 H isoform 3
NM_001202498.2	NP_001189427.1	ubiquitin-conjugating enzyme E2 H isoform 3
NM_182697.2	NP_874356.1	ubiquitin-conjugating enzyme E2 H isoform 2
NM_182697.3	NP_874356.1	ubiquitin-conjugating enzyme E2 H isoform 2
NM_003344.3	NP_003335.1	ubiquitin-conjugating enzyme E2 H isoform 1
NM_003344.4	NP_003335.1	ubiquitin-conjugating enzyme E2 H isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2H	NP_003335.1
Pan troglodytes (chimpanzee)	UBE2H	XP_001144807.2
Gallus gallus (chicken)	UBE2H	NP_001025818.1
Danio rerio (zebrafish)	ube2h	NP_958897.1
Drosophila melanogaster (fruit fly)	Ubc-E2H	NP_727234.1
Caenorhabditis elegans (roundworm)	ubc-8	NP_500245.2
Bos taurus (cattle)	UBE2H	NP_001032703.1
Rattus norvegicus (Norway rat)	Ube2h	NP_001165598.1
Schizosaccharomyces pombe (fission yeast)	ubc8	NP_596617.1
Neurospora crassa	NCU10046	XP_957953.1
Arabidopsis thaliana (thale cress)	UBC5	NP_564817.2
Arabidopsis thaliana (thale cress)	UBC4	NP_568589.1
Magnaporthe oryzae (rice blast fungus)	MGG_00600	XP_003718439.1
Xenopus tropicalis (tropical clawed frog)	ube2h	NP_001004909.1
	UBE2H	XP_001093822.1
Canis lupus familiaris (dog)	UBE2H	XP_857419.1
Eremothecium gossypii	AGOS_AEL045W	NP_984816.1
Arabidopsis thaliana (thale cress)	UBC6	NP_566062.1
Mus musculus (house mouse)	Ube2h	NP_033485.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001068	XP_318292.1
Saccharomyces cerevisiae (baker's yeast)	UBC8	NP_010904.2
Kluyveromyces lactis	KLLA0A11198g	XP_451487.1
Oryza sativa (rice)	Os10g0447100	NP_001064719.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Corominas R, Yang X, Lin GN, Kang S, Shen Y, Ghamsari L, Broly M, Rodriguez M, Tam S, Trigg SA, Fan C, Yi S, Tasan M, Lemmens I, Kuang X, Zhao N, Malhotra D, Michaelson JJ, Vacic V, Calderwood MA, Roth FP, Tavernier J, Horvath S, Salehi-Ashtiani K, Korkin D, Sebat J, Hill DE, Hao T, Vidal M, Iakoucheva LM
Nature communications53650(2014 Apr)

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Doyle JM, Gao J, Wang J, Yang M, Potts PR
Molecular cell39(6)963-74(2010 Sep)

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
David Y, Ziv T, Admon A, Navon A
The Journal of biological chemistry285(12)8595-604(2010 Mar)

Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1.
Lausen J, Pless O, Leonard F, Kuvardina ON, Koch B, Leutz A
The Journal of biological chemistry285(8)5338-46(2010 Feb)

Association study of the ubiquitin conjugating enzyme gene UBE2H in sporadic ALS.
Martin I, Vourc'h P, Mah? M, Th?pault RA, Antar C, V?drine S, Praline J, Camu W, Andres CR, Corcia P,
Amyotrophic lateral sclerosis : official publication of the World Federation of Neurology Research Group on Motor Neuron Diseases10(5-6)432-5(2009 Oct-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tal1 expression activated UBE2H expression, whereas Tal1 knock-down reduced UBE2H expression and ubiquitin transfer activity.
Title: Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1.

Results suggest that the UBE2H gene is not implicated in sporadic ALS.
Title: Association study of the ubiquitin conjugating enzyme gene UBE2H in sporadic ALS.

Observational study of gene-disease association. (HuGE Navigator)
Title: A common variant in DRD3 receptor is associated with autism spectrum disorder.

UBE2H gene could be one of the 7q-susceptibility loci for autistic disorder.
Title: Mutation screening and association study of the UBE2H gene on chromosome 7q32 in autistic disorder.

The following UBE2H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21861	NM_001202498.2 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22651	NM_182697.3 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu23115	NM_003344.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu21861	NM_001202498.1	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22651	NM_182697.2	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu23115	NM_003344.3	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2H ( NM_001202498.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189427.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21861
Clone ID Related Accession (Same CDS sequence)	NM_001202498.1 , NM_001202498.2
Accession Version	NM_001202498.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI088146.1, BC006277.2, AC084865.3 and AK026094.1. Transcript Variant: This variant (3) has an alternate splice site in the 5' region, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (3) has a shorter N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI088146.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCT 
CAGTTATTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAATA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA ATACGCCACG 
GAGGAGGCGC TGAAAGAACA GGAAGAGGGT ACCGGGGACA GCTCATCGGA GAGCTCTATG 
TCTGACTTTT CCGAAGATGA GGCCCAGGAT ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189427.1
CDS	220..561
Translation	MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_001202498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202498.1

ORF Insert Sequence:

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCT 
CAGTTATTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAATA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA ATACGCCACG 
GAGGAGGCGC TGAAAGAACA GGAAGAGGGT ACCGGGGACA GCTCATCGGA GAGCTCTATG 
TCTGACTTTT CCGAAGATGA GGCCCAGGAT ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2H ( NM_001202498.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189427.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21861
Clone ID Related Accession (Same CDS sequence)	NM_001202498.1 , NM_001202498.2
Accession Version	NM_001202498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073320.8, BI088146.1, BC006277.2, AC084865.3 and AK026094.1. On May 31, 2019 this sequence version replaced NM_001202498.1. Transcript Variant: This variant (3) has an alternate splice site in the 5' region, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (3) has a shorter N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI088146.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCT 
CAGTTATTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAATA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA ATACGCCACG 
GAGGAGGCGC TGAAAGAACA GGAAGAGGGT ACCGGGGACA GCTCATCGGA GAGCTCTATG 
TCTGACTTTT CCGAAGATGA GGCCCAGGAT ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189427.1
CDS	237..578
Translation	MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_001202498.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202498.2

ORF Insert Sequence:

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCT 
CAGTTATTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAATA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA ATACGCCACG 
GAGGAGGCGC TGAAAGAACA GGAAGAGGGT ACCGGGGACA GCTCATCGGA GAGCTCTATG 
TCTGACTTTT CCGAAGATGA GGCCCAGGAT ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2H ( NM_182697.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_874356.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22651
Clone ID Related Accession (Same CDS sequence)	NM_182697.2 , NM_182697.3
Accession Version	NM_182697.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073320.8, AY302138.1, AC084865.3 and AK026094.1. On Feb 3, 2011 this sequence version replaced NM_182697.1. Transcript Variant: This variant (2) lacks two consecutive in-frame exons in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.233601.1, SRR1803614.138003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCTCAG 
TTATTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAATACAA GCAGAAAATT AAAGAGTACA TCCAGAAATA CGCCACGGAG 
GAGGCGCTGA AAGAACAGGA AGAGGGTACC GGGGACAGCT CATCGGAGAG CTCTATGTCT 
GACTTTTCCG AAGATGAGGC CCAGGATATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_874356.1
CDS	406..864
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_182697.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182697.2

ORF Insert Sequence:

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCTCAG 
TTATTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAATACAA GCAGAAAATT AAAGAGTACA TCCAGAAATA CGCCACGGAG 
GAGGCGCTGA AAGAACAGGA AGAGGGTACC GGGGACAGCT CATCGGAGAG CTCTATGTCT 
GACTTTTCCG AAGATGAGGC CCAGGATATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2H ( NM_182697.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_874356.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22651
Clone ID Related Accession (Same CDS sequence)	NM_182697.2 , NM_182697.3
Accession Version	NM_182697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073320.8, AY302138.1, AC084865.3 and AK026094.1. On May 31, 2019 this sequence version replaced NM_182697.2. Transcript Variant: This variant (2) lacks two consecutive in-frame exons in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.233601.1, SRR1803614.138003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCTCAG 
TTATTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAATACAA GCAGAAAATT AAAGAGTACA TCCAGAAATA CGCCACGGAG 
GAGGCGCTGA AAGAACAGGA AGAGGGTACC GGGGACAGCT CATCGGAGAG CTCTATGTCT 
GACTTTTCCG AAGATGAGGC CCAGGATATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_874356.1
CDS	406..864
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_182697.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182697.3

ORF Insert Sequence:

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCTCAG 
TTATTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAATACAA GCAGAAAATT AAAGAGTACA TCCAGAAATA CGCCACGGAG 
GAGGCGCTGA AAGAACAGGA AGAGGGTACC GGGGACAGCT CATCGGAGAG CTCTATGTCT 
GACTTTTCCG AAGATGAGGC CCAGGATATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2H ( NM_003344.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003335.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23115
Clone ID Related Accession (Same CDS sequence)	NM_003344.3 , NM_003344.4
Accession Version	NM_003344.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073320.8, AB451490.1, AC084865.3 and AK026094.1. On Feb 3, 2011 this sequence version replaced NM_003344.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.234534.1, SRR1803614.40744.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCT CAGTTATTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAATA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA ATACGCCACG GAGGAGGCGC TGAAAGAACA GGAAGAGGGT 
ACCGGGGACA GCTCATCGGA GAGCTCTATG TCTGACTTTT CCGAAGATGA GGCCCAGGAT 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003335.1
CDS	406..957
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_003344.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003344.3

ORF Insert Sequence:

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCT CAGTTATTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAATA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA ATACGCCACG GAGGAGGCGC TGAAAGAACA GGAAGAGGGT 
ACCGGGGACA GCTCATCGGA GAGCTCTATG TCTGACTTTT CCGAAGATGA GGCCCAGGAT 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2H ( NM_003344.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003335.1 Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23115
Clone ID Related Accession (Same CDS sequence)	NM_003344.3 , NM_003344.4
Accession Version	NM_003344.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 H isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073320.8, AB451490.1, AC084865.3 and AK026094.1. On May 17, 2019 this sequence version replaced NM_003344.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.234534.1, SRR1660803.11792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355621.8/ ENSP00000347836.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCT CAGTTATTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAATA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA ATACGCCACG GAGGAGGCGC TGAAAGAACA GGAAGAGGGT 
ACCGGGGACA GCTCATCGGA GAGCTCTATG TCTGACTTTT CCGAAGATGA GGCCCAGGAT 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003335.1
CDS	406..957
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_003344.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 H (UBE2H), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003344.4

ORF Insert Sequence:

ATGTCATCTC CCAGTCCGGG CAAGAGGCGG ATGGACACGG ACGTGGTCAA GCTCATCGAG 
AGTAAACATG AGGTTACGAT CCTGGGAGGA CTTAATGAAT TTGTAGTGAA GTTTTATGGA 
CCACAAGGAA CACCATATGA AGGCGGAGTA TGGAAAGTTA GAGTGGACCT ACCTGATAAA 
TACCCTTTCA AATCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCT CAGTTATTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAATA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA ATACGCCACG GAGGAGGCGC TGAAAGAACA GGAAGAGGGT 
ACCGGGGACA GCTCATCGGA GAGCTCTATG TCTGACTTTT CCGAAGATGA GGCCCAGGAT 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Nature communications53650(2014 Apr)
Corominas R,Yang X,Lin GN,Kang S,Shen Y,Ghamsari L,Broly M,Rodriguez M,Tam S,Trigg SA,Fan C,Yi S,Tasan M,Lemmens I,Kuang X,Zhao N,Malhotra D,Michaelson JJ,Vacic V,Calderwood MA,Roth FP,Tavernier J,Horvath S,Salehi-Ashtiani K,Korkin D,Sebat J,Hill DE,Hao T,Vidal M,Iakoucheva LM

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Molecular cell39(6)963-74(2010 Sep)
Doyle JM,Gao J,Wang J,Yang M,Potts PR

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
The Journal of biological chemistry285(12)8595-604(2010 Mar)
David Y,Ziv T,Admon A,Navon A

Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1.
The Journal of biological chemistry285(8)5338-46(2010 Feb)
Lausen J,Pless O,Leonard F,Kuvardina ON,Koch B,Leutz A

Association study of the ubiquitin conjugating enzyme gene UBE2H in sporadic ALS.
Amyotrophic lateral sclerosis : official publication of the World Federation of Neurology Research Group on Motor Neuron Diseases10(5-6)432-5(2009 Oct-Dec)
Martin I,Vourc'h P,Mah? M,Th?pault RA,Antar C,V?drine S,Praline J,Camu W,Andres CR,Corcia P,

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

UBE2H cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報