UBE2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2B
Entrez Gene ID	7320
Full Name	ubiquitin conjugating enzyme E2 B
Synonyms	E2-17kDa,HHR6B,HR6B,RAD6B,UBC2
General protein information	Preferred Names ubiquitin conjugating enzyme E2 B Names ubiquitin-conjugating enzyme E2 B E2 protein E2 ubiquitin-conjugating enzyme B RAD6 homolog B ubiquitin carrier protein B ubiquitin conjugating enzyme E2B ubiquitin-conjugating enzyme E2-17 kDa ubiquitin-conjugating enzyme E2B (RAD6 homolog) ubiquitin-protein ligase B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.1
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008].
Disorder MIM:	179095
Disorder Html:	?Male infertility (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003337.3	NP_003328.1	ubiquitin-conjugating enzyme E2 B
NM_003337.4	NP_003328.1	ubiquitin-conjugating enzyme E2 B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2B	XP_517935.2
Bos taurus (cattle)	UBE2B	NP_001032536.1
Rattus norvegicus (Norway rat)	Ube2b	NP_112400.1
Neurospora crassa	NCU09731	XP_964433.2
	UBE2B	XP_001109004.1
Danio rerio (zebrafish)	ube2b	NP_001002747.1
Saccharomyces cerevisiae (baker's yeast)	RAD6	NP_011457.1
Kluyveromyces lactis	KLLA0C05632g	XP_452450.1
Homo sapiens (human)	UBE2B	NP_003328.1
Mus musculus (house mouse)	Ube2b	NP_033484.3
Xenopus tropicalis (tropical clawed frog)	ube2b	NP_001005124.1
Canis lupus familiaris (dog)	UBE2B	XP_850657.1
Gallus gallus (chicken)	LOC100857678	XP_004945057.1
Schizosaccharomyces pombe (fission yeast)	rhp6	NP_592876.1
Magnaporthe oryzae (rice blast fungus)	MGG_01756	XP_003714754.1
Eremothecium gossypii	AGOS_AAR156C	NP_982699.1

Gene Ontology
Bibliography

Related articles in PubMed

RAD6 promotes DNA repair and stem cell signaling in ovarian cancer and is a promising therapeutic target to prevent and treat acquired chemoresistance.
Somasagara RR, Spencer SM, Tripathi K, Clark DW, Mani C, Madeira da Silva L, Scalici J, Kothayer H, Westwell AD, Rocconi RP, Palle K
Oncogene36(48)6680-6690(2017 Nov)

Pharmacological targeting of RAD6 enzyme-mediated translesion synthesis overcomes resistance to platinum-based drugs.
Sanders MA, Haynes B, Nangia-Makker P, Polin LA, Shekhar MP
The Journal of biological chemistry292(25)10347-10363(2017 06)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Interactome Analysis Reveals a Novel Role for RAD6 in the Regulation of Proteasome Activity and Localization in Response to DNA Damage.
An H, Yang L, Wang C, Gan Z, Gu H, Zhang T, Huang X, Liu Y, Li Y, Chang SJ, Lai J, Li YB, Chen S, Sun FL
Molecular and cellular biology37(6)(2017 Mar)

Role of E2-RING Interactions in Governing RNF4-Mediated Substrate Ubiquitination.
DiBello A, Datta AB, Zhang X, Wolberger C
Journal of molecular biology428(23)4639-4650(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RAD6 is upregulated in response to chemotherapy and significantly correlated with expression of ovarian cancer (OC) stem cell signaling genes ALDH1A1 and SOX2 and poor prognosis of OC patients.
Title: RAD6 promotes DNA repair and stem cell signaling in ovarian cancer and is a promising therapeutic target to prevent and treat acquired chemoresistance.

These data deepen insights into the vital role of RAD6/translesion synthesis in platinum drug tolerance and reveal clinical benefits of targeting RAD6 with SMI#9 for managing chemoresistant cancers.
Title: Pharmacological targeting of RAD6 enzyme-mediated translesion synthesis overcomes resistance to platinum-based drugs.

RAD6 promotes proteasome activity and nuclear translocation by enhancing the degradation of PSMF1 and the lamin B receptor.
Title: Interactome Analysis Reveals a Novel Role for RAD6 in the Regulation of Proteasome Activity and Localization in Response to DNA Damage.

These results point to an important role of the affinity between RNF4 and its cognate RAD6B or UBCH5B in governing the multiplicity of substrate ubiquitination.
Title: Role of E2-RING Interactions in Governing RNF4-Mediated Substrate Ubiquitination.

In conclusion, we reveal that miR-455-5p expression is regulated by the TGF-beta-dependent pathway, which subsequently leads to UBE2B down-regulation and contributes to oral cancer tumourigenesis.
Title: Up-regulation of miR-455-5p by the TGF-?-SMAD signalling axis promotes the proliferation of oral squamous cancer cells by targeting UBE2B.

The following UBE2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27652	NM_003337.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27652	NM_003337.3	Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2B ( NM_003337.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003328.1 Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.

Price & Availability↑

CloneID	OHu27652
Clone ID Related Accession (Same CDS sequence)	NM_003337.3 , NM_003337.4
Accession Version	NM_003337.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA523817.1, AK312012.1, M74525.1, BU954901.1 and T88933.1. This sequence is a reference standard in the RefSeqGene project. On Apr 17, 2014 this sequence version replaced NM_003337.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M74525.1, SRR1803615.257995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGACCC CGGCCCGGAG GAGGCTCATG CGGGATTTCA AGCGGTTACA AGAGGACCCA 
CCTGTGGGTG TCAGTGGCGC ACCATCTGAA AACAACATCA TGCAGTGGAA TGCAGTTATA 
TTTGGACCAG AAGGGACACC TTTTGAAGAT GGTACTTTTA AACTAGTAAT AGAATTTTCT 
GAAGAATATC CAAATAAACC ACCAACTGTT AGGTTTTTAT CCAAAATGTT TCATCCAAAT 
GTGTATGCTG ATGGTAGCAT ATGTTTAGAT ATCCTTCAGA ATCGATGGAG TCCAACATAT 
GATGTATCTT CTATCTTAAC ATCAATTCAG TCTCTGCTGG ATGAACCGAA TCCTAACAGT 
CCAGCCAATA GCCAGGCAGC ACAGCTTTAT CAGGAAAACA AACGAGAATA TGAGAAAAGA 
GTTTCGGCCA TTGTTGAACA AAGCTGGAAT GATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003328.1
CDS	421..879
Translation	MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Target ORF information:

RefSeq Version	NM_003337.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003337.3

ORF Insert Sequence:

ATGTCGACCC CGGCCCGGAG GAGGCTCATG CGGGATTTCA AGCGGTTACA AGAGGACCCA 
CCTGTGGGTG TCAGTGGCGC ACCATCTGAA AACAACATCA TGCAGTGGAA TGCAGTTATA 
TTTGGACCAG AAGGGACACC TTTTGAAGAT GGTACTTTTA AACTAGTAAT AGAATTTTCT 
GAAGAATATC CAAATAAACC ACCAACTGTT AGGTTTTTAT CCAAAATGTT TCATCCAAAT 
GTGTATGCTG ATGGTAGCAT ATGTTTAGAT ATCCTTCAGA ATCGATGGAG TCCAACATAT 
GATGTATCTT CTATCTTAAC ATCAATTCAG TCTCTGCTGG ATGAACCGAA TCCTAACAGT 
CCAGCCAATA GCCAGGCAGC ACAGCTTTAT CAGGAAAACA AACGAGAATA TGAGAAAAGA 
GTTTCGGCCA TTGTTGAACA AAGCTGGAAT GATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2B ( NM_003337.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003328.1 Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.

Price & Availability↑

CloneID	OHu27652
Clone ID Related Accession (Same CDS sequence)	NM_003337.3 , NM_003337.4
Accession Version	NM_003337.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312012.1, M74525.1, BU954901.1 and T88933.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_003337.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.241935.1, SRR1803613.64513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265339.7/ ENSP00000265339.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGACCC CGGCCCGGAG GAGGCTCATG CGGGATTTCA AGCGGTTACA AGAGGACCCA 
CCTGTGGGTG TCAGTGGCGC ACCATCTGAA AACAACATCA TGCAGTGGAA TGCAGTTATA 
TTTGGACCAG AAGGGACACC TTTTGAAGAT GGTACTTTTA AACTAGTAAT AGAATTTTCT 
GAAGAATATC CAAATAAACC ACCAACTGTT AGGTTTTTAT CCAAAATGTT TCATCCAAAT 
GTGTATGCTG ATGGTAGCAT ATGTTTAGAT ATCCTTCAGA ATCGATGGAG TCCAACATAT 
GATGTATCTT CTATCTTAAC ATCAATTCAG TCTCTGCTGG ATGAACCGAA TCCTAACAGT 
CCAGCCAATA GCCAGGCAGC ACAGCTTTAT CAGGAAAACA AACGAGAATA TGAGAAAAGA 
GTTTCGGCCA TTGTTGAACA AAGCTGGAAT GATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003328.1
CDS	28..486
Translation	MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Target ORF information:

RefSeq Version	NM_003337.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003337.4

ORF Insert Sequence:

ATGTCGACCC CGGCCCGGAG GAGGCTCATG CGGGATTTCA AGCGGTTACA AGAGGACCCA 
CCTGTGGGTG TCAGTGGCGC ACCATCTGAA AACAACATCA TGCAGTGGAA TGCAGTTATA 
TTTGGACCAG AAGGGACACC TTTTGAAGAT GGTACTTTTA AACTAGTAAT AGAATTTTCT 
GAAGAATATC CAAATAAACC ACCAACTGTT AGGTTTTTAT CCAAAATGTT TCATCCAAAT 
GTGTATGCTG ATGGTAGCAT ATGTTTAGAT ATCCTTCAGA ATCGATGGAG TCCAACATAT 
GATGTATCTT CTATCTTAAC ATCAATTCAG TCTCTGCTGG ATGAACCGAA TCCTAACAGT 
CCAGCCAATA GCCAGGCAGC ACAGCTTTAT CAGGAAAACA AACGAGAATA TGAGAAAAGA 
GTTTCGGCCA TTGTTGAACA AAGCTGGAAT GATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RAD6 promotes DNA repair and stem cell signaling in ovarian cancer and is a promising therapeutic target to prevent and treat acquired chemoresistance.
Oncogene36(48)6680-6690(2017 Nov)
Somasagara RR,Spencer SM,Tripathi K,Clark DW,Mani C,Madeira da Silva L,Scalici J,Kothayer H,Westwell AD,Rocconi RP,Palle K

Pharmacological targeting of RAD6 enzyme-mediated translesion synthesis overcomes resistance to platinum-based drugs.
The Journal of biological chemistry292(25)10347-10363(2017 06)
Sanders MA,Haynes B,Nangia-Makker P,Polin LA,Shekhar MP

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Interactome Analysis Reveals a Novel Role for RAD6 in the Regulation of Proteasome Activity and Localization in Response to DNA Damage.
Molecular and cellular biology37(6)(2017 Mar)
An H,Yang L,Wang C,Gan Z,Gu H,Zhang T,Huang X,Liu Y,Li Y,Chang SJ,Lai J,Li YB,Chen S,Sun FL

Role of E2-RING Interactions in Governing RNF4-Mediated Substrate Ubiquitination.
Journal of molecular biology428(23)4639-4650(2016 Nov)
DiBello A,Datta AB,Zhang X,Wolberger C

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UBE2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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