HIRA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIRA
Entrez Gene ID	7290
Full Name	histone cell cycle regulator
Synonyms	DGCR1,TUP1,TUPLE1
General protein information	Preferred Names histone cell cycle regulator Names protein HIRA DiGeorge critical region gene 1 HIR histone cell cycle regulation defective homolog A TUP1-like enhancer of split protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008].
Disorder MIM:	600237

mRNA and Protein(s)

mRNA	Protein	Name
NM_003325.3	NP_003316.3	protein HIRA
NM_003325.4	NP_003316.3	protein HIRA

Pathways from BioSystems

Homologies

Homo sapiens (human)	HIRA	NP_003316.3
Bos taurus (cattle)	LOC101906870	XP_005218364.1
Mus musculus (house mouse)	Hira	NP_034565.2
Danio rerio (zebrafish)	hira	XP_696478.2
	HIRA	XP_001112873.2
Drosophila melanogaster (fruit fly)	Hira	NP_572401.2
Arabidopsis thaliana (thale cress)	HIRA	NP_001190009.1
Xenopus tropicalis (tropical clawed frog)	LOC100495450	XP_002931950.2
Pan troglodytes (chimpanzee)	HIRA	XP_001165585.1
Canis lupus familiaris (dog)	HIRA	XP_543550.2
Rattus norvegicus (Norway rat)	Hira	NP_001129232.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009488	XP_310209.4
Oryza sativa (rice)	Os09g0567700	NP_001063964.1
Gallus gallus (chicken)	HIRA	XP_004945677.1
Caenorhabditis elegans (roundworm)	K10D2.1	NP_498101.2

Gene Ontology
Bibliography

Related articles in PubMed

Histone chaperone HIRA deposits histone H3.3 onto foreign viral DNA and contributes to anti-viral intrinsic immunity.
Rai TS, Glass M, Cole JJ, Rather MI, Marsden M, Neilson M, Brock C, Humphreys IR, Everett RD, Adams PD
Nucleic acids research45(20)11673-11683(2017 Nov)

H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements.
Zink LM, Delbarre E, Eberl HC, Keilhauer EC, B?nisch C, P?nzeler S, Bartkuhn M, Collas P, Mann M, Hake SB
Nucleic acids research45(10)5691-5706(2017 Jun)

RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells.
Zhang H, Gan H, Wang Z, Lee JH, Zhou H, Ordog T, Wold MS, Ljungman M, Zhang Z
Molecular cell65(2)272-284(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these.
Title: Histone chaperone HIRA deposits histone H3.3 onto foreign viral DNA and contributes to anti-viral intrinsic immunity.

H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements.
Title: H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements.

PHB has an unexpected nuclear role in human embryonic stem cells that is required for self-renewal and that it acts with HIRA in chromatin organization to link epigenetic organization to a metabolic circuit.
Title: PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation.
Title: RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells.

The abnormal lower expression of the HIRA gene in the myocardium may participate in the pathogenesis of Tetralogy of Fallot.

The following HIRA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIRA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19128	NM_003325.4 Latest version!	Homo sapiens histone cell cycle regulator (HIRA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu19128	NM_003325.3	Homo sapiens histone cell cycle regulator (HIRA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIRA ( NM_003325.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003316.3 Homo sapiens histone cell cycle regulator (HIRA), mRNA.

Price & Availability↑

CloneID	OHu19128
Clone ID Related Accession (Same CDS sequence)	NM_003325.3 , NM_003325.4
Accession Version	NM_003325.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	protein HIRA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X89887.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2002 this sequence version replaced NM_003325.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X89887.1, BC039835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGCTCC TGAAGCCGAC CTGGGTCAAC CACAATGGCA AGCCGATTTT TTCAGTTGAT 
ATTCACCCTG ACGGGACCAA GTTCGCAACT GGAGGACAAG GGCAGGATTC TGGGAAGGTT 
GTGATCTGGA ATATGTCTCC AGTCCTCCAG GAGGATGACG AGAAGGATGA AAATATTCCC 
AAGATGCTTT GCCAGATGGA CAATCACTTA GCATGTGTGA ACTGTGTGCG GTGGTCAAAC 
AGTGGGATGT ATTTAGCTTC TGGGGGAGAT GACAAACTGA TTATGGTGTG GAAGCGGGCT 
ACGTACATCG GCCCCAGCAC CGTGTTCGGC TCCAGTGGTA AGCTTGCCAA TGTGGAGCAG 
TGGCGGTGTG TCTCTATCCT CCGGAATCAT TCAGGCGATG TGATGGATGT AGCATGGTCT 
CCCCACGATG CCTGGCTAGC CTCATGCAGC GTGGATAACA CTGTCGTCAT CTGGAATGCT 
GTAAAGTTCC CAGAAATTCT AGCTACTCTG AGAGGTCATT CTGGCTTGGT CAAAGGGTTG 
ACATGGGACC CTGTTGGTAA ATACATAGCT TCTCAAGCTG ATGACCGCAG CCTAAAGGTG 
TGGAGGACGC TGGACTGGCA GTTGGAGACC AGCATCACCA AGCCTTTTGA TGAGTGTGGA 
GGAACGACCC ATGTGTTGCG GCTCAGCTGG TCACCTGATG GGCATTACCT GGTGTCTGCC 
CATGCCATGA ACAACTCAGG CCCCACTGCC CAGATCATCG AACGGGAGGG ATGGAAGACC 
AACATGGACT TTGTTGGGCA CCGGAAAGCT GTGACTGTCG TGAAATTCAA CCCAAAAATC 
TTCAAAAAGA AGCAGAAGAA TGGGAGTTCT GCGAAGCCTA GCTGCCCGTA CTGCTGCTGT 
GCTGTTGGCA GCAAGGACCG CTCGCTTTCT GTCTGGCTCA CATGTCTGAA ACGGCCGCTG 
GTGGTCATCC ATGAACTGTT TGACAAATCC ATCATGGATA TTTCCTGGAC TCTGAATGGG 
CTGGGCATCT TGGTATGCTC TATGGACGGC TCTGTGGCAT TCCTCGACTT CTCCCAGGAT 
GAGCTTGGCG ATCCCCTGAG CGAGGAGGAG AAGAGCCGCA TTCACCAGTC CACCTATGGC 
AAGAGCCTAG CCATCATGAC CGAGGCCCAG CTCTCCACAG CCGTCATTGA GAACCCTGAG 
ATGCTCAAGT ACCAGCGAAG GCAGCAGCAG CAGCAGCTGG ACCAGAAGAG TGCTGCGACC 
AGGGAGATGG GCTCAGCCAC CTCAGTCGCA GGCGTTGTCA ACGGGGAGAG TCTTGAAGAT 
ATCAGGAAGA ATCTTTTGAA GAAACAAGTT GAGACTCGGA CAGCAGATGG CCGGAGAAGA 
ATCACGCCTC TCTGCATAGC ACAGCTGGAC ACTGGGGACT TCTCCACGGC ATTCTTTAAC 
AGCATCCCCC TCTCGGGCTC CCTGGCGGGC ACCATGCTCT CTTCTCATAG CAGTCCACAG 
CTACTGCCAC TGGACTCCAG TACCCCTAAC TCCTTCGGCG CCTCGAAGCC TTGCACAGAG 
CCTGTGGTGG CTGCCAGTGC CAGACCTGCA GGCGATTCTG TCAATAAAGA CAGTATGAAT 
GCTACCTCTA CTCCTGCTGC ATTGTCACCT TCTGTGTTAA CGACCCCGTC CAAGATCGAA 
CCCATGAAAG CGTTTGACTC CCGGTTCACA GAGCGGTCCA AAGCCACACC AGGTGCTCCT 
GCCCTGACCA GCATGACTCC GACAGCTGTG GAAAGGTTAA AAGAGCAGAA CCTTGTGAAA 
GAGCTGAGGC CCCGAGACCT CCTGGAGAGC AGCAGTGACA GCGATGAGAA AGTCCCTTTG 
GCTAAGGCTT CCTCACTGTC CAAGCGAAAA CTTGAGCTTG AGGTAGAGAC AGTAGAGAAG 
AAGAAGAAAG GGCGGCCTCG GAAGGACTCT CGTCTCATGC CTGTGTCTCT GTCTGTCCAG 
TCTCCAGCTG CCCTAACCGC AGAGAAGGAG GCCATGTGTC TGTCTGCACC AGCACTTGCA 
CTGAAGCTGC CAATTCCAAG CCCCCAGAGA GCATTCACCC TCCAGGTCAG CTCCGATCCT 
TCCATGTACA TTGAGGTGGA GAATGAAGTG ACAGTGGTGG GGGGCGTGAA GCTGAGCCGC 
CTGAAGTGCA ACCGGGAAGG GAAGGAGTGG GAGACGGTAC TCACCAGCCG GATCCTCACT 
GCTGCGGGCA GCTGTGACGT GGTGTGTGTC GCCTGTGAAA AAAGGATGCT GTCAGTGTTC 
TCCACCTGTG GTCGCCGTCT CCTCTCTCCC ATCCTCCTGC CATCCCCGAT CTCTACTTTG 
CATTGCACAG GCTCCTACGT CATGGCGCTC ACCGCTGCAG CCACACTCTC TGTCTGGGAT 
GTTCACAGAC AGGTGGTTGT GGTGAAAGAA GAGTCTCTAC ACTCCATCCT GGCAGGAAGT 
GATATGACGG TATCACAGAT CTTGCTGACG CAGCATGGAA TCCCAGTAAT GAACCTGTCC 
GATGGGAAGG CGTACTGCTT TAATCCGTCA CTTTCCACAT GGAACCTGGT TTCTGACAAG 
CAGGACTCAC TGGCTCAGTG TGCAGACTTT AGGAGCAGCC TGCCATCCCA GGACGCCATG 
CTGTGCTCAG GACCGTTAGC CATAATCCAG GGCCGCACCT CCAACTCGGG AAGGCAGGCT 
GCCCGGCTCT TCTCCGTGCC TCATGTGGTG CAGCAAGAGA CCACCCTGGC CTACCTAGAG 
AACCAGGTGG CAGCAGCACT CACCCTGCAG TCCAGCCACG AGTACCGCCA TTGGCTCCTC 
GTCTACGCAC GGTACCTCGT AAACGAAGGG TTTGAATACC GACTTCGAGA AATATGCAAG 
GACTTACTGG GTCCGGTTCA CTACTCCACT GGAAGCCAGT GGGAGTCAAC AGTAGTGGGT 
CTGCGGAAGA GGGAGCTGCT GAAGGAGCTG CTACCAGTCA TCGGGCAGAA CCTCCGATTC 
CAGCGCCTCT TCACCGAGTG TCAGGAACAG CTCGACATCC TGAGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003316.3
CDS	221..3274
Translation	MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAATREMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAGTMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIEPMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRKLELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLSDGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVGLRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK

Target ORF information:

RefSeq Version	NM_003325.3
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cell cycle regulator (HIRA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003325.3

ORF Insert Sequence:

ATGAAGCTCC TGAAGCCGAC CTGGGTCAAC CACAATGGCA AGCCGATTTT TTCAGTTGAT 
ATTCACCCTG ACGGGACCAA GTTCGCAACT GGAGGACAAG GGCAGGATTC TGGGAAGGTT 
GTGATCTGGA ATATGTCTCC AGTCCTCCAG GAGGATGACG AGAAGGATGA AAATATTCCC 
AAGATGCTTT GCCAGATGGA CAATCACTTA GCATGTGTGA ACTGTGTGCG GTGGTCAAAC 
AGTGGGATGT ATTTAGCTTC TGGGGGAGAT GACAAACTGA TTATGGTGTG GAAGCGGGCT 
ACGTACATCG GCCCCAGCAC CGTGTTCGGC TCCAGTGGTA AGCTTGCCAA TGTGGAGCAG 
TGGCGGTGTG TCTCTATCCT CCGGAATCAT TCAGGCGATG TGATGGATGT AGCATGGTCT 
CCCCACGATG CCTGGCTAGC CTCATGCAGC GTGGATAACA CTGTCGTCAT CTGGAATGCT 
GTAAAGTTCC CAGAAATTCT AGCTACTCTG AGAGGTCATT CTGGCTTGGT CAAAGGGTTG 
ACATGGGACC CTGTTGGTAA ATACATAGCT TCTCAAGCTG ATGACCGCAG CCTAAAGGTG 
TGGAGGACGC TGGACTGGCA GTTGGAGACC AGCATCACCA AGCCTTTTGA TGAGTGTGGA 
GGAACGACCC ATGTGTTGCG GCTCAGCTGG TCACCTGATG GGCATTACCT GGTGTCTGCC 
CATGCCATGA ACAACTCAGG CCCCACTGCC CAGATCATCG AACGGGAGGG ATGGAAGACC 
AACATGGACT TTGTTGGGCA CCGGAAAGCT GTGACTGTCG TGAAATTCAA CCCAAAAATC 
TTCAAAAAGA AGCAGAAGAA TGGGAGTTCT GCGAAGCCTA GCTGCCCGTA CTGCTGCTGT 
GCTGTTGGCA GCAAGGACCG CTCGCTTTCT GTCTGGCTCA CATGTCTGAA ACGGCCGCTG 
GTGGTCATCC ATGAACTGTT TGACAAATCC ATCATGGATA TTTCCTGGAC TCTGAATGGG 
CTGGGCATCT TGGTATGCTC TATGGACGGC TCTGTGGCAT TCCTCGACTT CTCCCAGGAT 
GAGCTTGGCG ATCCCCTGAG CGAGGAGGAG AAGAGCCGCA TTCACCAGTC CACCTATGGC 
AAGAGCCTAG CCATCATGAC CGAGGCCCAG CTCTCCACAG CCGTCATTGA GAACCCTGAG 
ATGCTCAAGT ACCAGCGAAG GCAGCAGCAG CAGCAGCTGG ACCAGAAGAG TGCTGCGACC 
AGGGAGATGG GCTCAGCCAC CTCAGTCGCA GGCGTTGTCA ACGGGGAGAG TCTTGAAGAT 
ATCAGGAAGA ATCTTTTGAA GAAACAAGTT GAGACTCGGA CAGCAGATGG CCGGAGAAGA 
ATCACGCCTC TCTGCATAGC ACAGCTGGAC ACTGGGGACT TCTCCACGGC ATTCTTTAAC 
AGCATCCCCC TCTCGGGCTC CCTGGCGGGC ACCATGCTCT CTTCTCATAG CAGTCCACAG 
CTACTGCCAC TGGACTCCAG TACCCCTAAC TCCTTCGGCG CCTCGAAGCC TTGCACAGAG 
CCTGTGGTGG CTGCCAGTGC CAGACCTGCA GGCGATTCTG TCAATAAAGA CAGTATGAAT 
GCTACCTCTA CTCCTGCTGC ATTGTCACCT TCTGTGTTAA CGACCCCGTC CAAGATCGAA 
CCCATGAAAG CGTTTGACTC CCGGTTCACA GAGCGGTCCA AAGCCACACC AGGTGCTCCT 
GCCCTGACCA GCATGACTCC GACAGCTGTG GAAAGGTTAA AAGAGCAGAA CCTTGTGAAA 
GAGCTGAGGC CCCGAGACCT CCTGGAGAGC AGCAGTGACA GCGATGAGAA AGTCCCTTTG 
GCTAAGGCTT CCTCACTGTC CAAGCGAAAA CTTGAGCTTG AGGTAGAGAC AGTAGAGAAG 
AAGAAGAAAG GGCGGCCTCG GAAGGACTCT CGTCTCATGC CTGTGTCTCT GTCTGTCCAG 
TCTCCAGCTG CCCTAACCGC AGAGAAGGAG GCCATGTGTC TGTCTGCACC AGCACTTGCA 
CTGAAGCTGC CAATTCCAAG CCCCCAGAGA GCATTCACCC TCCAGGTCAG CTCCGATCCT 
TCCATGTACA TTGAGGTGGA GAATGAAGTG ACAGTGGTGG GGGGCGTGAA GCTGAGCCGC 
CTGAAGTGCA ACCGGGAAGG GAAGGAGTGG GAGACGGTAC TCACCAGCCG GATCCTCACT 
GCTGCGGGCA GCTGTGACGT GGTGTGTGTC GCCTGTGAAA AAAGGATGCT GTCAGTGTTC 
TCCACCTGTG GTCGCCGTCT CCTCTCTCCC ATCCTCCTGC CATCCCCGAT CTCTACTTTG 
CATTGCACAG GCTCCTACGT CATGGCGCTC ACCGCTGCAG CCACACTCTC TGTCTGGGAT 
GTTCACAGAC AGGTGGTTGT GGTGAAAGAA GAGTCTCTAC ACTCCATCCT GGCAGGAAGT 
GATATGACGG TATCACAGAT CTTGCTGACG CAGCATGGAA TCCCAGTAAT GAACCTGTCC 
GATGGGAAGG CGTACTGCTT TAATCCGTCA CTTTCCACAT GGAACCTGGT TTCTGACAAG 
CAGGACTCAC TGGCTCAGTG TGCAGACTTT AGGAGCAGCC TGCCATCCCA GGACGCCATG 
CTGTGCTCAG GACCGTTAGC CATAATCCAG GGCCGCACCT CCAACTCGGG AAGGCAGGCT 
GCCCGGCTCT TCTCCGTGCC TCATGTGGTG CAGCAAGAGA CCACCCTGGC CTACCTAGAG 
AACCAGGTGG CAGCAGCACT CACCCTGCAG TCCAGCCACG AGTACCGCCA TTGGCTCCTC 
GTCTACGCAC GGTACCTCGT AAACGAAGGG TTTGAATACC GACTTCGAGA AATATGCAAG 
GACTTACTGG GTCCGGTTCA CTACTCCACT GGAAGCCAGT GGGAGTCAAC AGTAGTGGGT 
CTGCGGAAGA GGGAGCTGCT GAAGGAGCTG CTACCAGTCA TCGGGCAGAA CCTCCGATTC 
CAGCGCCTCT TCACCGAGTG TCAGGAACAG CTCGACATCC TGAGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIRA ( NM_003325.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003316.3 Homo sapiens histone cell cycle regulator (HIRA), mRNA.

Price & Availability↑

CloneID	OHu19128
Clone ID Related Accession (Same CDS sequence)	NM_003325.3 , NM_003325.4
Accession Version	NM_003325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	protein HIRA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000079.1, AC000092.1 and AC000085.5. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_003325.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039835.1, X89887.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263208.5/ ENSP00000263208.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGCTCC TGAAGCCGAC CTGGGTCAAC CACAATGGCA AGCCGATTTT TTCAGTTGAT 
ATTCACCCTG ACGGGACCAA GTTCGCAACT GGAGGACAAG GGCAGGATTC TGGGAAGGTT 
GTGATCTGGA ATATGTCTCC AGTCCTCCAG GAGGATGACG AGAAGGATGA AAATATTCCC 
AAGATGCTTT GCCAGATGGA CAATCACTTA GCATGTGTGA ACTGTGTGCG GTGGTCAAAC 
AGTGGGATGT ATTTAGCTTC TGGGGGAGAT GACAAACTGA TTATGGTGTG GAAGCGGGCT 
ACGTACATCG GCCCCAGCAC CGTGTTCGGC TCCAGTGGTA AGCTTGCCAA TGTGGAGCAG 
TGGCGGTGTG TCTCTATCCT CCGGAATCAT TCAGGCGATG TGATGGATGT AGCATGGTCT 
CCCCACGATG CCTGGCTAGC CTCATGCAGC GTGGATAACA CTGTCGTCAT CTGGAATGCT 
GTAAAGTTCC CAGAAATTCT AGCTACTCTG AGAGGTCATT CTGGCTTGGT CAAAGGGTTG 
ACATGGGACC CTGTTGGTAA ATACATAGCT TCTCAAGCTG ATGACCGCAG CCTAAAGGTG 
TGGAGGACGC TGGACTGGCA GTTGGAGACC AGCATCACCA AGCCTTTTGA TGAGTGTGGA 
GGAACGACCC ATGTGTTGCG GCTCAGCTGG TCACCTGATG GGCATTACCT GGTGTCTGCC 
CATGCCATGA ACAACTCAGG CCCCACTGCC CAGATCATCG AACGGGAGGG ATGGAAGACC 
AACATGGACT TTGTTGGGCA CCGGAAAGCT GTGACTGTCG TGAAATTCAA CCCAAAAATC 
TTCAAAAAGA AGCAGAAGAA TGGGAGTTCT GCGAAGCCTA GCTGCCCGTA CTGCTGCTGT 
GCTGTTGGCA GCAAGGACCG CTCGCTTTCT GTCTGGCTCA CATGTCTGAA ACGGCCGCTG 
GTGGTCATCC ATGAACTGTT TGACAAATCC ATCATGGATA TTTCCTGGAC TCTGAATGGG 
CTGGGCATCT TGGTATGCTC TATGGACGGC TCTGTGGCAT TCCTCGACTT CTCCCAGGAT 
GAGCTTGGCG ATCCCCTGAG CGAGGAGGAG AAGAGCCGCA TTCACCAGTC CACCTATGGC 
AAGAGCCTAG CCATCATGAC CGAGGCCCAG CTCTCCACAG CCGTCATTGA GAACCCTGAG 
ATGCTCAAGT ACCAGCGAAG GCAGCAGCAG CAGCAGCTGG ACCAGAAGAG TGCTGCGACC 
AGGGAGATGG GCTCAGCCAC CTCAGTCGCA GGCGTTGTCA ACGGGGAGAG TCTTGAAGAT 
ATCAGGAAGA ATCTTTTGAA GAAACAAGTT GAGACTCGGA CAGCAGATGG CCGGAGAAGA 
ATCACGCCTC TCTGCATAGC ACAGCTGGAC ACTGGGGACT TCTCCACGGC ATTCTTTAAC 
AGCATCCCCC TCTCGGGCTC CCTGGCGGGC ACCATGCTCT CTTCTCATAG CAGTCCACAG 
CTACTGCCAC TGGACTCCAG TACCCCTAAC TCCTTCGGCG CCTCGAAGCC TTGCACAGAG 
CCTGTGGTGG CTGCCAGTGC CAGACCTGCA GGCGATTCTG TCAATAAAGA CAGTATGAAT 
GCTACCTCTA CTCCTGCTGC ATTGTCACCT TCTGTGTTAA CGACCCCGTC CAAGATCGAA 
CCCATGAAAG CGTTTGACTC CCGGTTCACA GAGCGGTCCA AAGCCACACC AGGTGCTCCT 
GCCCTGACCA GCATGACTCC GACAGCTGTG GAAAGGTTAA AAGAGCAGAA CCTTGTGAAA 
GAGCTGAGGC CCCGAGACCT CCTGGAGAGC AGCAGTGACA GCGATGAGAA AGTCCCTTTG 
GCTAAGGCTT CCTCACTGTC CAAGCGAAAA CTTGAGCTTG AGGTAGAGAC AGTAGAGAAG 
AAGAAGAAAG GGCGGCCTCG GAAGGACTCT CGTCTCATGC CTGTGTCTCT GTCTGTCCAG 
TCTCCAGCTG CCCTAACCGC AGAGAAGGAG GCCATGTGTC TGTCTGCACC AGCACTTGCA 
CTGAAGCTGC CAATTCCAAG CCCCCAGAGA GCATTCACCC TCCAGGTCAG CTCCGATCCT 
TCCATGTACA TTGAGGTGGA GAATGAAGTG ACAGTGGTGG GGGGCGTGAA GCTGAGCCGC 
CTGAAGTGCA ACCGGGAAGG GAAGGAGTGG GAGACGGTAC TCACCAGCCG GATCCTCACT 
GCTGCGGGCA GCTGTGACGT GGTGTGTGTC GCCTGTGAAA AAAGGATGCT GTCAGTGTTC 
TCCACCTGTG GTCGCCGTCT CCTCTCTCCC ATCCTCCTGC CATCCCCGAT CTCTACTTTG 
CATTGCACAG GCTCCTACGT CATGGCGCTC ACCGCTGCAG CCACACTCTC TGTCTGGGAT 
GTTCACAGAC AGGTGGTTGT GGTGAAAGAA GAGTCTCTAC ACTCCATCCT GGCAGGAAGT 
GATATGACGG TATCACAGAT CTTGCTGACG CAGCATGGAA TCCCAGTAAT GAACCTGTCC 
GATGGGAAGG CGTACTGCTT TAATCCGTCA CTTTCCACAT GGAACCTGGT TTCTGACAAG 
CAGGACTCAC TGGCTCAGTG TGCAGACTTT AGGAGCAGCC TGCCATCCCA GGACGCCATG 
CTGTGCTCAG GACCGTTAGC CATAATCCAG GGCCGCACCT CCAACTCGGG AAGGCAGGCT 
GCCCGGCTCT TCTCCGTGCC TCATGTGGTG CAGCAAGAGA CCACCCTGGC CTACCTAGAG 
AACCAGGTGG CAGCAGCACT CACCCTGCAG TCCAGCCACG AGTACCGCCA TTGGCTCCTC 
GTCTACGCAC GGTACCTCGT AAACGAAGGG TTTGAATACC GACTTCGAGA AATATGCAAG 
GACTTACTGG GTCCGGTTCA CTACTCCACT GGAAGCCAGT GGGAGTCAAC AGTAGTGGGT 
CTGCGGAAGA GGGAGCTGCT GAAGGAGCTG CTACCAGTCA TCGGGCAGAA CCTCCGATTC 
CAGCGCCTCT TCACCGAGTG TCAGGAACAG CTCGACATCC TGAGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003316.3
CDS	258..3311
Translation	MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAATREMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAGTMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIEPMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRKLELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLSDGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVGLRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK

Target ORF information:

RefSeq Version	NM_003325.4
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cell cycle regulator (HIRA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003325.4

ORF Insert Sequence:

ATGAAGCTCC TGAAGCCGAC CTGGGTCAAC CACAATGGCA AGCCGATTTT TTCAGTTGAT 
ATTCACCCTG ACGGGACCAA GTTCGCAACT GGAGGACAAG GGCAGGATTC TGGGAAGGTT 
GTGATCTGGA ATATGTCTCC AGTCCTCCAG GAGGATGACG AGAAGGATGA AAATATTCCC 
AAGATGCTTT GCCAGATGGA CAATCACTTA GCATGTGTGA ACTGTGTGCG GTGGTCAAAC 
AGTGGGATGT ATTTAGCTTC TGGGGGAGAT GACAAACTGA TTATGGTGTG GAAGCGGGCT 
ACGTACATCG GCCCCAGCAC CGTGTTCGGC TCCAGTGGTA AGCTTGCCAA TGTGGAGCAG 
TGGCGGTGTG TCTCTATCCT CCGGAATCAT TCAGGCGATG TGATGGATGT AGCATGGTCT 
CCCCACGATG CCTGGCTAGC CTCATGCAGC GTGGATAACA CTGTCGTCAT CTGGAATGCT 
GTAAAGTTCC CAGAAATTCT AGCTACTCTG AGAGGTCATT CTGGCTTGGT CAAAGGGTTG 
ACATGGGACC CTGTTGGTAA ATACATAGCT TCTCAAGCTG ATGACCGCAG CCTAAAGGTG 
TGGAGGACGC TGGACTGGCA GTTGGAGACC AGCATCACCA AGCCTTTTGA TGAGTGTGGA 
GGAACGACCC ATGTGTTGCG GCTCAGCTGG TCACCTGATG GGCATTACCT GGTGTCTGCC 
CATGCCATGA ACAACTCAGG CCCCACTGCC CAGATCATCG AACGGGAGGG ATGGAAGACC 
AACATGGACT TTGTTGGGCA CCGGAAAGCT GTGACTGTCG TGAAATTCAA CCCAAAAATC 
TTCAAAAAGA AGCAGAAGAA TGGGAGTTCT GCGAAGCCTA GCTGCCCGTA CTGCTGCTGT 
GCTGTTGGCA GCAAGGACCG CTCGCTTTCT GTCTGGCTCA CATGTCTGAA ACGGCCGCTG 
GTGGTCATCC ATGAACTGTT TGACAAATCC ATCATGGATA TTTCCTGGAC TCTGAATGGG 
CTGGGCATCT TGGTATGCTC TATGGACGGC TCTGTGGCAT TCCTCGACTT CTCCCAGGAT 
GAGCTTGGCG ATCCCCTGAG CGAGGAGGAG AAGAGCCGCA TTCACCAGTC CACCTATGGC 
AAGAGCCTAG CCATCATGAC CGAGGCCCAG CTCTCCACAG CCGTCATTGA GAACCCTGAG 
ATGCTCAAGT ACCAGCGAAG GCAGCAGCAG CAGCAGCTGG ACCAGAAGAG TGCTGCGACC 
AGGGAGATGG GCTCAGCCAC CTCAGTCGCA GGCGTTGTCA ACGGGGAGAG TCTTGAAGAT 
ATCAGGAAGA ATCTTTTGAA GAAACAAGTT GAGACTCGGA CAGCAGATGG CCGGAGAAGA 
ATCACGCCTC TCTGCATAGC ACAGCTGGAC ACTGGGGACT TCTCCACGGC ATTCTTTAAC 
AGCATCCCCC TCTCGGGCTC CCTGGCGGGC ACCATGCTCT CTTCTCATAG CAGTCCACAG 
CTACTGCCAC TGGACTCCAG TACCCCTAAC TCCTTCGGCG CCTCGAAGCC TTGCACAGAG 
CCTGTGGTGG CTGCCAGTGC CAGACCTGCA GGCGATTCTG TCAATAAAGA CAGTATGAAT 
GCTACCTCTA CTCCTGCTGC ATTGTCACCT TCTGTGTTAA CGACCCCGTC CAAGATCGAA 
CCCATGAAAG CGTTTGACTC CCGGTTCACA GAGCGGTCCA AAGCCACACC AGGTGCTCCT 
GCCCTGACCA GCATGACTCC GACAGCTGTG GAAAGGTTAA AAGAGCAGAA CCTTGTGAAA 
GAGCTGAGGC CCCGAGACCT CCTGGAGAGC AGCAGTGACA GCGATGAGAA AGTCCCTTTG 
GCTAAGGCTT CCTCACTGTC CAAGCGAAAA CTTGAGCTTG AGGTAGAGAC AGTAGAGAAG 
AAGAAGAAAG GGCGGCCTCG GAAGGACTCT CGTCTCATGC CTGTGTCTCT GTCTGTCCAG 
TCTCCAGCTG CCCTAACCGC AGAGAAGGAG GCCATGTGTC TGTCTGCACC AGCACTTGCA 
CTGAAGCTGC CAATTCCAAG CCCCCAGAGA GCATTCACCC TCCAGGTCAG CTCCGATCCT 
TCCATGTACA TTGAGGTGGA GAATGAAGTG ACAGTGGTGG GGGGCGTGAA GCTGAGCCGC 
CTGAAGTGCA ACCGGGAAGG GAAGGAGTGG GAGACGGTAC TCACCAGCCG GATCCTCACT 
GCTGCGGGCA GCTGTGACGT GGTGTGTGTC GCCTGTGAAA AAAGGATGCT GTCAGTGTTC 
TCCACCTGTG GTCGCCGTCT CCTCTCTCCC ATCCTCCTGC CATCCCCGAT CTCTACTTTG 
CATTGCACAG GCTCCTACGT CATGGCGCTC ACCGCTGCAG CCACACTCTC TGTCTGGGAT 
GTTCACAGAC AGGTGGTTGT GGTGAAAGAA GAGTCTCTAC ACTCCATCCT GGCAGGAAGT 
GATATGACGG TATCACAGAT CTTGCTGACG CAGCATGGAA TCCCAGTAAT GAACCTGTCC 
GATGGGAAGG CGTACTGCTT TAATCCGTCA CTTTCCACAT GGAACCTGGT TTCTGACAAG 
CAGGACTCAC TGGCTCAGTG TGCAGACTTT AGGAGCAGCC TGCCATCCCA GGACGCCATG 
CTGTGCTCAG GACCGTTAGC CATAATCCAG GGCCGCACCT CCAACTCGGG AAGGCAGGCT 
GCCCGGCTCT TCTCCGTGCC TCATGTGGTG CAGCAAGAGA CCACCCTGGC CTACCTAGAG 
AACCAGGTGG CAGCAGCACT CACCCTGCAG TCCAGCCACG AGTACCGCCA TTGGCTCCTC 
GTCTACGCAC GGTACCTCGT AAACGAAGGG TTTGAATACC GACTTCGAGA AATATGCAAG 
GACTTACTGG GTCCGGTTCA CTACTCCACT GGAAGCCAGT GGGAGTCAAC AGTAGTGGGT 
CTGCGGAAGA GGGAGCTGCT GAAGGAGCTG CTACCAGTCA TCGGGCAGAA CCTCCGATTC 
CAGCGCCTCT TCACCGAGTG TCAGGAACAG CTCGACATCC TGAGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone chaperone HIRA deposits histone H3.3 onto foreign viral DNA and contributes to anti-viral intrinsic immunity.
Nucleic acids research45(20)11673-11683(2017 Nov)
Rai TS,Glass M,Cole JJ,Rather MI,Marsden M,Neilson M,Brock C,Humphreys IR,Everett RD,Adams PD

H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements.
Nucleic acids research45(10)5691-5706(2017 Jun)
Zink LM,Delbarre E,Eberl HC,Keilhauer EC,B?nisch C,P?nzeler S,Bartkuhn M,Collas P,Mann M,Hake SB

RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells.
Molecular cell65(2)272-284(2017 Jan)
Zhang H,Gan H,Wang Z,Lee JH,Zhou H,Ordog T,Wold MS,Ljungman M,Zhang Z

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HIRA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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