DEFA1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEFA1B
Entrez Gene ID	728358
Full Name	defensin alpha 1B
Synonyms	HNP-1,HP-1,HP1
General protein information	Preferred Names defensin alpha 1B Names neutrophil defensin 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042500.1	NP_001035965.1	neutrophil defensin 1 isoform 2 preproprotein
NM_001302265.1	NP_001289194.1	neutrophil defensin 1 isoform 1 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	DEFA1B	NP_001035965.1
Homo sapiens (human)	DEFA1	NP_004075.1
Homo sapiens (human)	DEFA3	NP_005208.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Human defensins facilitate local unfolding of thermodynamically unstable regions of bacterial protein toxins.
Kudryashova E, Quintyn R, Seveau S, Lu W, Wysocki VH, Kudryashov DS
Immunity41(5)709-21(2014 Nov)

Alpha-defensins 1-3 release by dendritic cells is reduced by estrogen.
Escribese MM, Rodr?guez-Garc?a M, Sperling R, Engel SM, Gallart T, Moran TM
Reproductive biology and endocrinology : RB&E9118(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

binding of HNP1 to affected bacterial toxins caused their local unfolding, potentiated their thermal melting and precipitation, exposed new regions for proteolysis, and increased susceptibility to collisional quenchers.
Title: Human defensins facilitate local unfolding of thermodynamically unstable regions of bacterial protein toxins.

Data show that H. pylori induces alpha-defensin release from granulocytes, which may be important in local host response to H. pylori infection in gastroduodenal diseases.
Title: Helicobacter pylori induces the release of alpha-defensin by human granulocytes.

DEFA1B encodes an antibacterial peptide that is bacteriocidal against S. aureus, E. coli, and P. aeruginosa.
Title: Defensins. Natural peptide antibiotics of human neutrophils.

The following DEFA1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEFA1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu12997	NM_001042500.1 Latest version!	Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu57229	NM_001302265.1 Latest version!	Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEFA1B ( NM_001042500.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035965.1 Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12997
Clone ID Related Accession (Same CDS sequence)	NM_001042500.1
Accession Version	NM_001042500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	neutrophil defensin 1 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF238378.5. On Jun 20, 2007 this sequence version replaced XM_001127929.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start site compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY252327.1, HY074599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162823 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2997278 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGACCC TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
CCACTCCAGG CAAGAGCTGA TGAGGTTGCT GCAGCCCCGG AGCAGATTGC AGCGGACATC 
CCAGAAGTGG TTGTTTCCCT TGCATGGGAC GAAAGCTTGG CTCCAAAGCA TCCAGGCTCA 
AGGAAAAACA TGGCCTGCTA TTGCAGAATA CCAGCGTGCA TTGCAGGAGA ACGTCGCTAT 
GGAACCTGCA TCTACCAGGG AAGACTCTGG GCATTCTGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035965.1
CDS	93..377
Translation	MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Target ORF information:

RefSeq Version	NM_001042500.1
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042500.1

ORF Insert Sequence:

ATGAGGACCC TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
CCACTCCAGG CAAGAGCTGA TGAGGTTGCT GCAGCCCCGG AGCAGATTGC AGCGGACATC 
CCAGAAGTGG TTGTTTCCCT TGCATGGGAC GAAAGCTTGG CTCCAAAGCA TCCAGGCTCA 
AGGAAAAACA TGGCCTGCTA TTGCAGAATA CCAGCGTGCA TTGCAGGAGA ACGTCGCTAT 
GGAACCTGCA TCTACCAGGG AAGACTCTGG GCATTCTGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEFA1B ( NM_001302265.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289194.1 Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu57229
Clone ID Related Accession (Same CDS sequence)	NM_001302265.1
Accession Version	NM_001302265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	neutrophil defensin 1 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF238378.5, HY073456.1 and HY252327.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY073456.1, AJ705305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162823 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2997278 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289194.1
CDS	116..421
Translation	MRMVTPAMRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Target ORF information:

RefSeq Version	NM_001302265.1
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302265.1

ORF Insert Sequence:

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Human defensins facilitate local unfolding of thermodynamically unstable regions of bacterial protein toxins.
Immunity41(5)709-21(2014 Nov)
Kudryashova E,Quintyn R,Seveau S,Lu W,Wysocki VH,Kudryashov DS

Alpha-defensins 1-3 release by dendritic cells is reduced by estrogen.
Reproductive biology and endocrinology : RB&E9118(2011 Aug)
Escribese MM,Rodr?guez-Garc?a M,Sperling R,Engel SM,Gallart T,Moran TM

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DEFA1B cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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