TTC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTC4
Entrez Gene ID	7268
Full Name	tetratricopeptide repeat domain 4
Synonyms	CNS1
General protein information	Preferred Names tetratricopeptide repeat domain 4 Names tetratricopeptide repeat protein 4 TPR repeat protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p32.3
Summary	This gene encodes a protein that contains tetratricopeptide (TPR) repeats, which often mediate protein-protein interactions and chaperone activity. The encoded protein interacts with heat shock proteins 70 and 90. Alternative splicing results in multiple transcript variants. Naturally-occuring readthrough transcription occurs from upstream gene MROH (maestro heat-like repeat family member 7) to this gene. [provided by RefSeq, Apr 2014].
Disorder MIM:	606753

mRNA and Protein(s)

mRNA	Protein	Name
NM_004623.5	NP_004614.3	tetratricopeptide repeat protein 4 isoform 1
NM_001291333.1	NP_001278262.1	tetratricopeptide repeat protein 4 isoform 2
NM_004623.4	NP_004614.3	tetratricopeptide repeat protein 4 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TTC4	NP_004614.3
Pan troglodytes (chimpanzee)	TTC4	XP_001153260.1
Mus musculus (house mouse)	Ttc4	NP_082485.1
Magnaporthe oryzae (rice blast fungus)	MGG_06164	XP_003712044.1
Drosophila melanogaster (fruit fly)	Dpit47	NP_001260729.1
Eremothecium gossypii	AGOS_AGL268C	NP_986399.1
Bos taurus (cattle)	TTC4	NP_001015554.1
Danio rerio (zebrafish)	ttc4	NP_001002122.1
Caenorhabditis elegans (roundworm)	C17G10.2	NP_495087.1
Kluyveromyces lactis	KLLA0B09966g	XP_451972.1
Schizosaccharomyces pombe (fission yeast)	cns1	NP_594238.1
Xenopus tropicalis (tropical clawed frog)	ttc4	XP_002931478.2
Canis lupus familiaris (dog)	TTC4	XP_853176.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012044	XP_320482.3
Saccharomyces cerevisiae (baker's yeast)	CNS1	NP_009713.1
Neurospora crassa	NCU06340	XP_962374.1
	TTC4	XP_001114145.1
Gallus gallus (chicken)	TTC4	XP_426665.3

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity.
Shang J, Xia T, Han QQ, Zhao X, Hu MM, Shu HB, Guo L
Proteomics(2017 Dec)

Two distinct nuclear localization signals in mammalian MSL1 regulate its function.
Dmitriev RI, Pestov NB, Shakhparonov MI, Okkelman IA
Journal of cellular biochemistry115(11)1967-73(2014 Nov)

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, Berger B, Gingras AC, Lindquist S
Cell158(2)434-448(2014 Jul)

Molecular cochaperones: tumor growth and cancer treatment.
Calderwood SK
Scientifica2013217513(2013)

The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells.
Crevel G, Bennett D, Cotterill S
PloS one3(3)e0001737(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies identi fi ed a novel signal of nuclear localization (NLS) in all MSL1 protein isoforms and found that the combination of both NLS allows for its intra-nuclear focal accumulation and nuclear transport of TTC4 while all MSL1 isoforms affect H4K16Ac.
Title: Two distinct nuclear localization signals in mammalian MSL1 regulate its function.

TTC4 is highly expressed in malignant melanoma
Title: The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells.

The following TTC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19488	NM_004623.5 Latest version!	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu14473	NM_001291333.1 Latest version!	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu19488	NM_004623.4	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC4 ( NM_004623.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004614.3 Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19488
Clone ID Related Accession (Same CDS sequence)	NM_004623.5 , NM_004623.4
Accession Version	NM_004623.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	tetratricopeptide repeat protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025524.1, AK222562.1 and DB362353.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004623.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK025524.1, SRR1803614.121838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371281.4/ ENSP00000360329.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGCTAGGAAT ATCAGGCTCT CAGAAGCTGC CTGTGAGGAT 
GAAGATTCAG CCTCAGAAGG TCTAGGTGAG CTTTTCCTGG ATGGACTCAG CACTGAGAAC 
CCCCATGGAG CCAGGCTGAG TCTAGATGGC CAGGGCAGGC TGAGCTGGCC TGTGCTCTTT 
CTGTACCCAG AGTATGCCCA GTCGGACTTC ATCTCTGCTT TTCATGAGGA CTCCAGGTTT 
ATTGATCATC TAATGGTGAT GTTTGGTGAA ACACCCTCTT GGGACCTAGA GCAAAAATAT 
TGCCCTGATA ATTTGGAGGT CTACTTTGAG GATGAGGACA GGGCAGAACT ATACCGGGTG 
CCTGCCAAGA GCACCTTGCT ACAGGTTCTA CAGCACCAGA GGTACTTTGT AAAAGCCCTG 
ACACCAGCAT TTTTGGTCTG TGTAGGATCC TCTCCTTTTT GCAAGAATTT TCTCCGGGGG 
AGAAAGGTGT ACCAGATACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004614.3
CDS	49..1212
Translation	MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMSRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

Target ORF information:

RefSeq Version	NM_004623.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004623.5

ORF Insert Sequence:

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGCTAGGAAT ATCAGGCTCT CAGAAGCTGC CTGTGAGGAT 
GAAGATTCAG CCTCAGAAGG TCTAGGTGAG CTTTTCCTGG ATGGACTCAG CACTGAGAAC 
CCCCATGGAG CCAGGCTGAG TCTAGATGGC CAGGGCAGGC TGAGCTGGCC TGTGCTCTTT 
CTGTACCCAG AGTATGCCCA GTCGGACTTC ATCTCTGCTT TTCATGAGGA CTCCAGGTTT 
ATTGATCATC TAATGGTGAT GTTTGGTGAA ACACCCTCTT GGGACCTAGA GCAAAAATAT 
TGCCCTGATA ATTTGGAGGT CTACTTTGAG GATGAGGACA GGGCAGAACT ATACCGGGTG 
CCTGCCAAGA GCACCTTGCT ACAGGTTCTA CAGCACCAGA GGTACTTTGT AAAAGCCCTG 
ACACCAGCAT TTTTGGTCTG TGTAGGATCC TCTCCTTTTT GCAAGAATTT TCTCCGGGGG 
AGAAAGGTGT ACCAGATACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC4 ( NM_001291333.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278262.1 Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14473
Clone ID Related Accession (Same CDS sequence)	NM_001291333.1
Accession Version	NM_001291333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	tetratricopeptide repeat protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139244.21. Transcript Variant: This variant (2) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AL539823.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGTCTACTTT GAGGATGAGG ACAGGGCAGA ACTATACCGG 
GTGCCTGCCA AGAGCACCTT GCTACAGGTT CTACAGCACC AGAGGTACTT TGTAAAAGCC 
CTGACACCAG CATTTTTGGT CTGTGTAGGA TCCTCTCCTT TTTGCAAGAA TTTTCTCCGG 
GGGAGAAAGG TGTACCAGAT ACGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278262.1
CDS	88..954
Translation	MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMSRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

Target ORF information:

RefSeq Version	NM_001291333.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291333.1

ORF Insert Sequence:

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGTCTACTTT GAGGATGAGG ACAGGGCAGA ACTATACCGG 
GTGCCTGCCA AGAGCACCTT GCTACAGGTT CTACAGCACC AGAGGTACTT TGTAAAAGCC 
CTGACACCAG CATTTTTGGT CTGTGTAGGA TCCTCTCCTT TTTGCAAGAA TTTTCTCCGG 
GGGAGAAAGG TGTACCAGAT ACGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC4 ( NM_004623.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004614.3 Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19488
Clone ID Related Accession (Same CDS sequence)	NM_004623.5 , NM_004623.4
Accession Version	NM_004623.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Homo sapiens(human)
Product	tetratricopeptide repeat protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025524.1, AK222562.1 and DB362353.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_004623.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK025524.1, SRR1803611.203139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGCTAGGAAT ATCAGGCTCT CAGAAGCTGC CTGTGAGGAT 
GAAGATTCAG CCTCAGAAGG TCTAGGTGAG CTTTTCCTGG ATGGACTCAG CACTGAGAAC 
CCCCATGGAG CCAGGCTGAG TCTAGATGGC CAGGGCAGGC TGAGCTGGCC TGTGCTCTTT 
CTGTACCCAG AGTATGCCCA GTCGGACTTC ATCTCTGCTT TTCATGAGGA CTCCAGGTTT 
ATTGATCATC TAATGGTGAT GTTTGGTGAA ACACCCTCTT GGGACCTAGA GCAAAAATAT 
TGCCCTGATA ATTTGGAGGT CTACTTTGAG GATGAGGACA GGGCAGAACT ATACCGGGTG 
CCTGCCAAGA GCACCTTGCT ACAGGTTCTA CAGCACCAGA GGTACTTTGT AAAAGCCCTG 
ACACCAGCAT TTTTGGTCTG TGTAGGATCC TCTCCTTTTT GCAAGAATTT TCTCCGGGGG 
AGAAAGGTGT ACCAGATACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004614.3
CDS	88..1251
Translation	MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMSRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

Target ORF information:

RefSeq Version	NM_004623.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tetratricopeptide repeat domain 4 (TTC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004623.4

ORF Insert Sequence:

ATGGAACAAC CTGGGCAGGA TCCCACCTCA GACGACGTCA TGGACTCGTT CCTGGAAAAG 
TTCCAGAGCC AGCCTTACCG TGGCGGCTTT CATGAGGACC AGTGGGAGAA GGAATTTGAA 
AAGGTCCCCC TATTTATGTC GAGAGCGCCA TCAGAAATTG ATCCCAGGGA GAATCCTGAC 
TTGGCTTGTC TCCAGTCAAT TATTTTTGAT GAGGAGCGTT CTCCAGAAGA ACAGGCCAAG 
ACCTATAAAG ATGAGGGCAA TGATTACTTT AAAGAAAAAG ACTACAAGAA AGCTGTAATT 
TCATACACTG AAGGCTTAAA GAAGAAATGT GCAGATCCTG ATTTGAATGC TGTCCTTTAT 
ACCAACCGGG CAGCAGCACA GTACTATCTG GGCAATTTTC GTTCTGCTCT CAATGATGTG 
ACAGCTGCCA GAAAGCTAAA ACCCTGCCAC CTCAAAGCAA TAATAAGAGG TGCCTTATGC 
CATCTGGAAC TGAAACACTT TGCCGAGGCC GTGAACTGGT GTGATGAGGG ACTGCAAATA 
GATGCCAAAG AGAAGAAGCT TCTGGAAATG AGGGCTAAAG CAGACAAGCT GAAGCGAATT 
GAACAGAGGG ATGTGAGGAA AGCCAACTTG AAAGAAAAGA AGGAGAGGAA TCAGAATGAG 
GCTTTACTCC AGGCCATCAA GGCTAGGAAT ATCAGGCTCT CAGAAGCTGC CTGTGAGGAT 
GAAGATTCAG CCTCAGAAGG TCTAGGTGAG CTTTTCCTGG ATGGACTCAG CACTGAGAAC 
CCCCATGGAG CCAGGCTGAG TCTAGATGGC CAGGGCAGGC TGAGCTGGCC TGTGCTCTTT 
CTGTACCCAG AGTATGCCCA GTCGGACTTC ATCTCTGCTT TTCATGAGGA CTCCAGGTTT 
ATTGATCATC TAATGGTGAT GTTTGGTGAA ACACCCTCTT GGGACCTAGA GCAAAAATAT 
TGCCCTGATA ATTTGGAGGT CTACTTTGAG GATGAGGACA GGGCAGAACT ATACCGGGTG 
CCTGCCAAGA GCACCTTGCT ACAGGTTCTA CAGCACCAGA GGTACTTTGT AAAAGCCCTG 
ACACCAGCAT TTTTGGTCTG TGTAGGATCC TCTCCTTTTT GCAAGAATTT TCTCCGGGGG 
AGAAAGGTGT ACCAGATACG ATGA