INS-IGF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	INS-IGF2
Entrez Gene ID	723961
Full Name	INS-IGF2 readthrough
Synonyms	INSIGF
General protein information	Preferred Names INS-IGF2 readthrough Names insulin, isoform 2 INS-IGF2 readthrough transcript protein insulin- insulin-like growth factor 2 read-through product
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Summary	This locus includes two alternatively spliced read-through transcript variants which align to the INS gene in the 5' region and to the IGF2 gene in the 3' region. One transcript is predicted to encode a protein which shares the N-terminus with the INS protein but has a distinct and longer C-terminus, whereas the other transcript is a candidate for nonsense-mediated decay (NMD). The transcripts are imprinted and are paternally expressed in the limb and eye. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042376.2	NP_001035835.1	insulin, isoform 2 precursor
NM_001042376.3	NP_001035835.1	insulin, isoform 2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Meta-analysis of genome-wide association studies in African Americans provides insights into the genetic architecture of type 2 diabetes.
Ng MC, Shriner D, Chen BH, Li J, Chen WM, Guo X, Liu J, Bielinski SJ, Yanek LR, Nalls MA, Comeau ME, Rasmussen-Torvik LJ, Jensen RA, Evans DS, Sun YV, An P, Patel SR, Lu Y, Long J, Armstrong LL, Wagenknecht L, Yang L, Snively BM, Palmer ND, Mudgal P, Langefeld CD, Keene KL, Freedman BI, Mychaleckyj JC, Nayak U, Raffel LJ, Goodarzi MO, Chen YD, Taylor HA Jr, Correa A, Sims M, Couper D, Pankow JS, Boerwinkle E, Adeyemo A, Doumatey A, Chen G, Mathias RA, Vaidya D, Singleton AB, Zonderman AB, Igo RP Jr, Sedor JR, , Kabagambe EK, Siscovick DS, McKnight B, Rice K, Liu Y, Hsueh WC, Zhao W, Bielak LF, Kraja A, Province MA, Bottinger EP, Gottesman O, Cai Q, Zheng W, Blot WJ, Lowe WL, Pacheco JA, Crawford DC, , , Grundberg E, , Rich SS, Hayes MG, Shu XO, Loos RJ, Borecki IB, Peyser PA, Cummings SR, Psaty BM, Fornage M, Iyengar SK, Evans MK, Becker DM, Kao WH, Wilson JG, Rotter JI, Sale MM, Liu S, Rotimi CN, Bowden DW,
PLoS genetics10(8)e1004517(2014 Aug)

Autoimmunity against INS-IGF2 protein expressed in human pancreatic islets.
Kanatsuna N, Taneera J, Vaziri-Sani F, Wierup N, Larsson HE, Delli A, Sk?rstrand H, Balhuizen A, Bennet H, Steiner DF, T?rn C, Fex M, Lernmark ?
The Journal of biological chemistry288(40)29013-23(2013 Oct)

Periconception maternal smoking and low education are associated with methylation of INSIGF in children at the age of 17 months.
Obermann-Borst SA, Heijmans BT, Eilers PH, Tobi EW, Steegers EA, Slagboom PE, Steegers-Theunissen RP
Journal of developmental origins of health and disease3(5)315-20(2012 Oct)

Genome-wide association analysis of autoantibody positivity in type 1 diabetes cases.
Plagnol V, Howson JM, Smyth DJ, Walker N, Hafler JP, Wallace C, Stevens H, Jackson L, Simmonds MJ, , Bingley PJ, Gough SC, Todd JA
PLoS genetics7(8)e1002216(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified three known loci (TCF7L2, HMGA2 and KCNQ1) and two novel loci (HLA-B and INS-IGF2) at genome-wide significance (4.15 x 10(-94)
Title: Meta-analysis of genome-wide association studies in African Americans provides insights into the genetic architecture of type 2 diabetes.

INS-IGF2 and insulin may share autoantibody-binding sites, thus complicating the notion that insulin is the primary autoantigen in type 1 diabetes.
Title: Autoimmunity against INS-IGF2 protein expressed in human pancreatic islets.

Periconception maternal smoking and low education are associated with dna methylation on INSIGF in the very young child.
Title: Periconception maternal smoking and low education are associated with methylation of INSIGF in children at the age of 17 months.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following INS-IGF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INS-IGF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14598	NM_001042376.3 Latest version!	Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OHu14598	NM_001042376.2	Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

INS-IGF2 ( NM_001042376.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035835.1 Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14598
Clone ID Related Accession (Same CDS sequence)	NM_001042376.2 , NM_001042376.3
Accession Version	NM_001042376.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	insulin, isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ104204.1, AC132217.15 and BQ128162.1. This sequence is a reference standard in the RefSeqGene project. On Oct 8, 2010 this sequence version replaced NM_001042376.1. Transcript Variant: This variant (2, also known as INSIGF short) represents the shorter transcript and is protein-coding. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ104204.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 16531418 readthrough transcript :: includes exons from GeneID 3481, 3630 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGCCT CAGCTTTGTC CCTCTCCTCC TCCACGTCAA CCTGGCCAGA GGGTCTGGAC 
GCCACAGCCA GGGCACCCCC TGCTTTGGTG GTGACTGCTA ATATTGGCCA GGCCGGCGGA 
TCATCGTCCA GGCAGTTTCG GCAGAGAGCC TTGGGCACCA GTGACTCCCC GGTCCTCTTT 
ATCCACTGTC CAGGAGCTGC GGGGACTGCG CAGGGACTAG AGTACAGGGG CCGAAGAGTC 
ACCACCGAGC TTGTGTGGGA GGAGGTGGAT TCCAGCCCCC AGCCCCAGGG CTCTGAATCG 
CTGCCAGCTC AGCCCCCTGC CCAGCCTGCC CCACAGCCTG AGCCCCAGCA GGCCAGAGAG 
CCCAGTCCTG AGGTGAGCTG CTGTGGCCTG TGGCCCAGGC GACCCCAGCG CTCCCAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035835.1
CDS	60..662
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQASALSLSSSTSTWPEGLDATARAPPALVVTANIGQAGGSSSRQFRQRALGTSDSPVLFIHCPGAAGTAQGLEYRGRRVTTELVWEEVDSSPQPQGSESLPAQPPAQPAPQPEPQQAREPSPEVSCCGLWPRRPQRSQN

Target ORF information:

RefSeq Version	NM_001042376.2
Organism	Homo sapiens(human)
Definition	Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042376.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGCCT CAGCTTTGTC CCTCTCCTCC TCCACGTCAA CCTGGCCAGA GGGTCTGGAC 
GCCACAGCCA GGGCACCCCC TGCTTTGGTG GTGACTGCTA ATATTGGCCA GGCCGGCGGA 
TCATCGTCCA GGCAGTTTCG GCAGAGAGCC TTGGGCACCA GTGACTCCCC GGTCCTCTTT 
ATCCACTGTC CAGGAGCTGC GGGGACTGCG CAGGGACTAG AGTACAGGGG CCGAAGAGTC 
ACCACCGAGC TTGTGTGGGA GGAGGTGGAT TCCAGCCCCC AGCCCCAGGG CTCTGAATCG 
CTGCCAGCTC AGCCCCCTGC CCAGCCTGCC CCACAGCCTG AGCCCCAGCA GGCCAGAGAG 
CCCAGTCCTG AGGTGAGCTG CTGTGGCCTG TGGCCCAGGC GACCCCAGCG CTCCCAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS-IGF2 ( NM_001042376.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035835.1 Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14598
Clone ID Related Accession (Same CDS sequence)	NM_001042376.2 , NM_001042376.3
Accession Version	NM_001042376.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	insulin, isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ104204.1, AC132217.15 and BQ128162.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001042376.2. Transcript Variant: This variant (2, also known as INSIGF short) represents the shorter transcript and is protein-coding. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ104204.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 16531418 readthrough transcript :: includes exons from GeneID 3481, 3630 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGCCT CAGCTTTGTC CCTCTCCTCC TCCACGTCAA CCTGGCCAGA GGGTCTGGAC 
GCCACAGCCA GGGCACCCCC TGCTTTGGTG GTGACTGCTA ATATTGGCCA GGCCGGCGGA 
TCATCGTCCA GGCAGTTTCG GCAGAGAGCC TTGGGCACCA GTGACTCCCC GGTCCTCTTT 
ATCCACTGTC CAGGAGCTGC GGGGACTGCG CAGGGACTAG AGTACAGGGG CCGAAGAGTC 
ACCACCGAGC TTGTGTGGGA GGAGGTGGAT TCCAGCCCCC AGCCCCAGGG CTCTGAATCG 
CTGCCAGCTC AGCCCCCTGC CCAGCCTGCC CCACAGCCTG AGCCCCAGCA GGCCAGAGAG 
CCCAGTCCTG AGGTGAGCTG CTGTGGCCTG TGGCCCAGGC GACCCCAGCG CTCCCAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035835.1
CDS	60..662
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQASALSLSSSTSTWPEGLDATARAPPALVVTANIGQAGGSSSRQFRQRALGTSDSPVLFIHCPGAAGTAQGLEYRGRRVTTELVWEEVDSSPQPQGSESLPAQPPAQPAPQPEPQQAREPSPEVSCCGLWPRRPQRSQN

Target ORF information:

RefSeq Version	NM_001042376.3
Organism	Homo sapiens(human)
Definition	Homo sapiens INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042376.3

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGCCT CAGCTTTGTC CCTCTCCTCC TCCACGTCAA CCTGGCCAGA GGGTCTGGAC 
GCCACAGCCA GGGCACCCCC TGCTTTGGTG GTGACTGCTA ATATTGGCCA GGCCGGCGGA 
TCATCGTCCA GGCAGTTTCG GCAGAGAGCC TTGGGCACCA GTGACTCCCC GGTCCTCTTT 
ATCCACTGTC CAGGAGCTGC GGGGACTGCG CAGGGACTAG AGTACAGGGG CCGAAGAGTC 
ACCACCGAGC TTGTGTGGGA GGAGGTGGAT TCCAGCCCCC AGCCCCAGGG CTCTGAATCG 
CTGCCAGCTC AGCCCCCTGC CCAGCCTGCC CCACAGCCTG AGCCCCAGCA GGCCAGAGAG 
CCCAGTCCTG AGGTGAGCTG CTGTGGCCTG TGGCCCAGGC GACCCCAGCG CTCCCAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Meta-analysis of genome-wide association studies in African Americans provides insights into the genetic architecture of type 2 diabetes.
PLoS genetics10(8)e1004517(2014 Aug)
Ng MC,Shriner D,Chen BH,Li J,Chen WM,Guo X,Liu J,Bielinski SJ,Yanek LR,Nalls MA,Comeau ME,Rasmussen-Torvik LJ,Jensen RA,Evans DS,Sun YV,An P,Patel SR,Lu Y,Long J,Armstrong LL,Wagenknecht L,Yang L,Snively BM,Palmer ND,Mudgal P,Langefeld CD,Keene KL,Freedman BI,Mychaleckyj JC,Nayak U,Raffel LJ,Goodarzi MO,Chen YD,Taylor HA Jr,Correa A,Sims M,Couper D,Pankow JS,Boerwinkle E,Adeyemo A,Doumatey A,Chen G,Mathias RA,Vaidya D,Singleton AB,Zonderman AB,Igo RP Jr,Sedor JR,,Kabagambe EK,Siscovick DS,McKnight B,Rice K,Liu Y,Hsueh WC,Zhao W,Bielak LF,Kraja A,Province MA,Bottinger EP,Gottesman O,Cai Q,Zheng W,Blot WJ,Lowe WL,Pacheco JA,Crawford DC,,,Grundberg E,,Rich SS,Hayes MG,Shu XO,Loos RJ,Borecki IB,Peyser PA,Cummings SR,Psaty BM,Fornage M,Iyengar SK,Evans MK,Becker DM,Kao WH,Wilson JG,Rotter JI,Sale MM,Liu S,Rotimi CN,Bowden DW,

Autoimmunity against INS-IGF2 protein expressed in human pancreatic islets.
The Journal of biological chemistry288(40)29013-23(2013 Oct)
Kanatsuna N,Taneera J,Vaziri-Sani F,Wierup N,Larsson HE,Delli A,Sk?rstrand H,Balhuizen A,Bennet H,Steiner DF,T?rn C,Fex M,Lernmark ?

Periconception maternal smoking and low education are associated with methylation of INSIGF in children at the age of 17 months.
Journal of developmental origins of health and disease3(5)315-20(2012 Oct)
Obermann-Borst SA,Heijmans BT,Eilers PH,Tobi EW,Steegers EA,Slagboom PE,Steegers-Theunissen RP

Genome-wide association analysis of autoantibody positivity in type 1 diabetes cases.
PLoS genetics7(8)e1002216(2011 Aug)
Plagnol V,Howson JM,Smyth DJ,Walker N,Hafler JP,Wallace C,Stevens H,Jackson L,Simmonds MJ,,Bingley PJ,Gough SC,Todd JA

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INS-IGF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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