TRH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRH
Entrez Gene ID	7200
Full Name	thyrotropin releasing hormone
Synonyms	Pro-TRH,TRF
General protein information	Preferred Names thyrotropin releasing hormone Names thyrotropin releasing hormone TSH-releasing factor pro-thyrotropin-releasing hormone prothyroliberin protirelin thyrotropin-releasing factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.1
Summary	This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013].
Disorder MIM:	613879
Disorder Html:	Thyrotropin-releasing hormone deficiency, 275120 (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007117.4	NP_009048.1	thyrotropin releasing hormone preproprotein
NM_007117.5	NP_009048.1	thyrotropin releasing hormone preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRH	NP_009048.1
	TRH	XP_001093550.2
Mus musculus (house mouse)	Trh	NP_033452.2
Rattus norvegicus (Norway rat)	Trh	NP_037178.1
Pan troglodytes (chimpanzee)	TRH	XP_001144565.1
Bos taurus (cattle)	TRH	NP_001094691.1
Canis lupus familiaris (dog)	TRH	XP_005632182.1

Gene Ontology
Bibliography

Related articles in PubMed

TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP.
Brejchov? J, S?kora J, Osta?ov P, Merta L, Roubalov? L, Jan??ek J, Hof M, Svoboda P
Biochimica et biophysica acta1848(3)781-96(2015 Mar)

Biased paternal transmission of TRH variant to female Down syndrome probands: possible correlation with low breast cancer frequency.
Dey A, Chatterjee A, Maiti A, Mukherjee N, Sinha S, Panda CK, Mukhopadhyay K
The International journal of biological markers30(1)e142-7(2015 Feb)

The dynamic pituitary response to escalating-dose TRH stimulation test in hypothyroid patients treated with liothyronine or levothyroxine replacement therapy.
Yavuz S, Linderman JD, Smith S, Zhao X, Pucino F, Celi FS
The Journal of clinical endocrinology and metabolism98(5)E862-6(2013 May)

Thyrotropin-releasing hormone, luteinizing hormone-releasing hormone and substance P immuno-reactivity in post-mortem brain from cases of Alzheimer-type dementia and Down's syndrome.
Yates CM, Harmar AJ, Rosie R, Sheward J, de Levy GS, Simpson J, Maloney AF, Gordon A, Fink G
Brain research258(1)45-52(1983 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that decrease of cholesterol level impaired the functional coupling between the thyrotropin-releasing hormone receptor and the cognate G proteins.
Title: TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP.

TRH rs13097335 may have a protective role toward the development of breast cancer.
Title: Biased paternal transmission of TRH variant to female Down syndrome probands: possible correlation with low breast cancer frequency.

TRH inhibits melanin-concentrating hormone (MCH) neurons by increasing synaptic inhibition from local GABA neurons.
Title: Thyrotropin-releasing hormone (TRH) inhibits melanin-concentrating hormone neurons: implications for TRH-mediated anorexic and arousal actions.

This study introduces TRH as a novel, potent, selective, and evolutionarily highly conserved neuroendocrine factor controlling human pigmentation in situ.
Title: Thyrotropin-releasing hormone selectively stimulates human hair follicle pigmentation.

TRH, LH-RH and substance P are not affected in Alzheimer disease and Down's syndrome.
Title: Thyrotropin-releasing hormone, luteinizing hormone-releasing hormone and substance P immuno-reactivity in post-mortem brain from cases of Alzheimer-type dementia and Down's syndrome.

The following TRH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21186	NM_007117.5 Latest version!	Homo sapiens thyrotropin releasing hormone (TRH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu21186	NM_007117.4	Homo sapiens thyrotropin releasing hormone (TRH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRH ( NM_007117.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009048.1 Homo sapiens thyrotropin releasing hormone (TRH), mRNA.

Price & Availability↑

CloneID	OHu21186
Clone ID Related Accession (Same CDS sequence)	NM_007117.4 , NM_007117.5
Accession Version	NM_007117.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Homo sapiens(human)
Product	thyrotropin releasing hormone preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA515022.1, AK313473.1 and AC083906.23. This sequence is a reference standard in the RefSeqGene project. On May 2, 2013 this sequence version replaced NM_007117.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110515.2, AK313473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGGCC CTTGGTTGCT GCTCGCTCTG GCTTTGACCC TGAACCTGAC CGGTGTCCCC 
GGCGGCCGTG CTCAGCCAGA GGCGGCCCAG CAGGAGGCAG TGACGGCCGC GGAGCATCCG 
GGCCTGGATG ACTTCCTGCG CCAGGTGGAG CGCCTCCTCT TCCTCCGGGA AAACATCCAG 
CGGCTGCAAG GGGACCAGGG TGAGCACTCC GCGTCCCAGA TCTTTCAATC TGACTGGCTC 
TCCAAACGTC AGCATCCAGG CAAAAGAGAG GAGGAGGAGG AAGAGGGAGT TGAAGAAGAG 
GAAGAGGAAG AAGGGGGGGC TGTGGGACCC CACAAACGGC AGCACCCTGG CCGACGAGAA 
GATGAGGCTT CATGGTCAGT CGATGTAACC CAGCACAAGC GGCAGCATCC TGGCCGGCGC 
TCCCCCTGGC TTGCATATGC TGTCCCGAAG CGGCAGCACC CAGGCAGAAG GCTGGCAGAT 
CCCAAGGCTC AAAGGAGCTG GGAAGAAGAG GAGGAGGAGG AAGAGAGAGA GGAAGACCTG 
ATGCCTGAAA AACGCCAGCA TCCGGGCAAG AGGGCCCTGG GAGGCCCCTG TGGGCCCCAG 
GGAGCCTATG GTCAAGCGGG CCTTCTGCTG GGGCTCCTGG ATGACCTGAG TAGGAGCCAG 
GGAGCTGAGG AAAAGCGGCA GCACCCTGGT CGGCGGGCAG CCTGGGTCAG AGAGCCCCTG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009048.1
CDS	440..1168
Translation	MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE

Target ORF information:

RefSeq Version	NM_007117.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone (TRH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007117.4

ORF Insert Sequence:

ATGCCCGGCC CTTGGTTGCT GCTCGCTCTG GCTTTGACCC TGAACCTGAC CGGTGTCCCC 
GGCGGCCGTG CTCAGCCAGA GGCGGCCCAG CAGGAGGCAG TGACGGCCGC GGAGCATCCG 
GGCCTGGATG ACTTCCTGCG CCAGGTGGAG CGCCTCCTCT TCCTCCGGGA AAACATCCAG 
CGGCTGCAAG GGGACCAGGG TGAGCACTCC GCGTCCCAGA TCTTTCAATC TGACTGGCTC 
TCCAAACGTC AGCATCCAGG CAAAAGAGAG GAGGAGGAGG AAGAGGGAGT TGAAGAAGAG 
GAAGAGGAAG AAGGGGGGGC TGTGGGACCC CACAAACGGC AGCACCCTGG CCGACGAGAA 
GATGAGGCTT CATGGTCAGT CGATGTAACC CAGCACAAGC GGCAGCATCC TGGCCGGCGC 
TCCCCCTGGC TTGCATATGC TGTCCCGAAG CGGCAGCACC CAGGCAGAAG GCTGGCAGAT 
CCCAAGGCTC AAAGGAGCTG GGAAGAAGAG GAGGAGGAGG AAGAGAGAGA GGAAGACCTG 
ATGCCTGAAA AACGCCAGCA TCCGGGCAAG AGGGCCCTGG GAGGCCCCTG TGGGCCCCAG 
GGAGCCTATG GTCAAGCGGG CCTTCTGCTG GGGCTCCTGG ATGACCTGAG TAGGAGCCAG 
GGAGCTGAGG AAAAGCGGCA GCACCCTGGT CGGCGGGCAG CCTGGGTCAG AGAGCCCCTG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRH ( NM_007117.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_009048.1 Homo sapiens thyrotropin releasing hormone (TRH), mRNA.

Price & Availability↑

CloneID	OHu21186
Clone ID Related Accession (Same CDS sequence)	NM_007117.4 , NM_007117.5
Accession Version	NM_007117.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	thyrotropin releasing hormone preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313473.1 and AC083906.23. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_007117.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110515.2, AK313473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302649.4/ ENSP00000303452.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGGCC CTTGGTTGCT GCTCGCTCTG GCTTTGACCC TGAACCTGAC CGGTGTCCCC 
GGCGGCCGTG CTCAGCCAGA GGCGGCCCAG CAGGAGGCAG TGACGGCCGC GGAGCATCCG 
GGCCTGGATG ACTTCCTGCG CCAGGTGGAG CGCCTCCTCT TCCTCCGGGA AAACATCCAG 
CGGCTGCAAG GGGACCAGGG TGAGCACTCC GCGTCCCAGA TCTTTCAATC TGACTGGCTC 
TCCAAACGTC AGCATCCAGG CAAAAGAGAG GAGGAGGAGG AAGAGGGAGT TGAAGAAGAG 
GAAGAGGAAG AAGGGGGGGC TGTGGGACCC CACAAACGGC AGCACCCTGG CCGACGAGAA 
GATGAGGCTT CATGGTCAGT CGATGTAACC CAGCACAAGC GGCAGCATCC TGGCCGGCGC 
TCCCCCTGGC TTGCATATGC TGTCCCGAAG CGGCAGCACC CAGGCAGAAG GCTGGCAGAT 
CCCAAGGCTC AAAGGAGCTG GGAAGAAGAG GAGGAGGAGG AAGAGAGAGA GGAAGACCTG 
ATGCCTGAAA AACGCCAGCA TCCGGGCAAG AGGGCCCTGG GAGGCCCCTG TGGGCCCCAG 
GGAGCCTATG GTCAAGCGGG CCTTCTGCTG GGGCTCCTGG ATGACCTGAG TAGGAGCCAG 
GGAGCTGAGG AAAAGCGGCA GCACCCTGGT CGGCGGGCAG CCTGGGTCAG AGAGCCCCTG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009048.1
CDS	113..841
Translation	MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE

Target ORF information:

RefSeq Version	NM_007117.5
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone (TRH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007117.5

ORF Insert Sequence:

ATGCCCGGCC CTTGGTTGCT GCTCGCTCTG GCTTTGACCC TGAACCTGAC CGGTGTCCCC 
GGCGGCCGTG CTCAGCCAGA GGCGGCCCAG CAGGAGGCAG TGACGGCCGC GGAGCATCCG 
GGCCTGGATG ACTTCCTGCG CCAGGTGGAG CGCCTCCTCT TCCTCCGGGA AAACATCCAG 
CGGCTGCAAG GGGACCAGGG TGAGCACTCC GCGTCCCAGA TCTTTCAATC TGACTGGCTC 
TCCAAACGTC AGCATCCAGG CAAAAGAGAG GAGGAGGAGG AAGAGGGAGT TGAAGAAGAG 
GAAGAGGAAG AAGGGGGGGC TGTGGGACCC CACAAACGGC AGCACCCTGG CCGACGAGAA 
GATGAGGCTT CATGGTCAGT CGATGTAACC CAGCACAAGC GGCAGCATCC TGGCCGGCGC 
TCCCCCTGGC TTGCATATGC TGTCCCGAAG CGGCAGCACC CAGGCAGAAG GCTGGCAGAT 
CCCAAGGCTC AAAGGAGCTG GGAAGAAGAG GAGGAGGAGG AAGAGAGAGA GGAAGACCTG 
ATGCCTGAAA AACGCCAGCA TCCGGGCAAG AGGGCCCTGG GAGGCCCCTG TGGGCCCCAG 
GGAGCCTATG GTCAAGCGGG CCTTCTGCTG GGGCTCCTGG ATGACCTGAG TAGGAGCCAG 
GGAGCTGAGG AAAAGCGGCA GCACCCTGGT CGGCGGGCAG CCTGGGTCAG AGAGCCCCTG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP.
Biochimica et biophysica acta1848(3)781-96(2015 Mar)
Brejchov? J,S?kora J,Osta?ov P,Merta L,Roubalov? L,Jan??ek J,Hof M,Svoboda P

Biased paternal transmission of TRH variant to female Down syndrome probands: possible correlation with low breast cancer frequency.
The International journal of biological markers30(1)e142-7(2015 Feb)
Dey A,Chatterjee A,Maiti A,Mukherjee N,Sinha S,Panda CK,Mukhopadhyay K

The dynamic pituitary response to escalating-dose TRH stimulation test in hypothyroid patients treated with liothyronine or levothyroxine replacement therapy.
The Journal of clinical endocrinology and metabolism98(5)E862-6(2013 May)
Yavuz S,Linderman JD,Smith S,Zhao X,Pucino F,Celi FS

Thyrotropin-releasing hormone, luteinizing hormone-releasing hormone and substance P immuno-reactivity in post-mortem brain from cases of Alzheimer-type dementia and Down's syndrome.
Brain research258(1)45-52(1983 Jan)
Yates CM,Harmar AJ,Rosie R,Sheward J,de Levy GS,Simpson J,Maloney AF,Gordon A,Fink G

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TRH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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