ACTG2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTG2
Entrez Gene ID	72
Full Name	actin, gamma 2, smooth muscle, enteric
Synonyms	ACT,ACTA3,ACTE,ACTL3,ACTSG,VSCM
General protein information	Preferred Names actin, gamma 2, smooth muscle, enteric Names actin, gamma-enteric smooth muscle actin-like protein alpha-actin-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010].
Disorder MIM:	102545
Disorder Html:	Visceral myopathy, 155310 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199893.2	NP_001186822.1	actin, gamma-enteric smooth muscle isoform 2 precursor
NM_001615.3	NP_001606.1	actin, gamma-enteric smooth muscle isoform 1 precursor
NM_001615.4	NP_001606.1	actin, gamma-enteric smooth muscle isoform 1 precursor
NM_001199893.1	NP_001186822.1	actin, gamma-enteric smooth muscle isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTG2	XP_533002.2
Bos taurus (cattle)	ACTG2	NP_001013610.1
Gallus gallus (chicken)	ACTG2	NP_990503.1
Arabidopsis thaliana (thale cress)	ACT12	NP_190236.1
	ACTG2	XP_001105970.1
Mus musculus (house mouse)	Actg2	NP_033740.2
Danio rerio (zebrafish)	acta2	NP_997785.1
Arabidopsis thaliana (thale cress)	ACT4	NP_001078769.1
Homo sapiens (human)	ACTG2	NP_001606.1
Pan troglodytes (chimpanzee)	ACTG2	XP_001152416.2
Rattus norvegicus (Norway rat)	Actg2	NP_037025.1
Drosophila melanogaster (fruit fly)	Act79B	NP_001262200.1
Oryza sativa (rice)	Os10g0510000	NP_001065034.1
Xenopus tropicalis (tropical clawed frog)	acta2	NP_001011250.1

Gene Ontology
Bibliography

Related articles in PubMed

Prenatal diagnosis of chronic intestinal pseudo-obstruction and paternal somatic mosaicism for the ACTG2 pathogenic variant.
Milunsky A, Lazier J, Baldwin C, Young C, Primack D, Milunsky JM
Prenatal diagnosis37(12)1254-1256(2017 Dec)

Diagnosis of Chronic Intestinal Pseudo-obstruction and Megacystis by Sequencing the ACTG2 Gene.
Milunsky A, Baldwin C, Zhang X, Primack D, Curnow A, Milunsky J
Journal of pediatric gastroenterology and nutrition65(4)384-387(2017 Oct)

Identification of ACTG2 functions as a promoter gene in hepatocellular carcinoma cells migration and tumor metastasis.
Wu Y, Liu ZG, Shi MQ, Yu HZ, Jiang XY, Yang AH, Fu XS, Xu Y, Yang S, Ni H, Shen SJ, Li WD
Biochemical and biophysical research communications491(2)537-544(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACTG2 boosts the metastatic potential of hepatocellular carcinoma in a Notch1-dependent manner.
Title: Identification of ACTG2 functions as a promoter gene in hepatocellular carcinoma cells migration and tumor metastasis.

R257 variant in the ACTG2 appear to be more frequent in populations of Asian ancestry; mutation of this locus could cause alterations of the intestinal and bladder smooth muscle filaments.
Title: Mutation in Actin ?-2 Responsible for Megacystis Microcolon Intestinal Hypoperistalsis Syndrome in 4 Chinese Patients.

Missense variants in ACTG2 were identified in the patients with either megacystis-microcolon-intestinal hypoperistalsis syndrome or intestinal pseudo-obstruction.
Title: Variants of the ACTG2 gene correlate with degree of severity and presence of megacystis in chronic intestinal pseudo-obstruction.

ACTG2 is expressed in a fraction of small intestinal neuroendocrine tumors, can inhibit cell growth in vitro, and is positively regulated by miR-145.
Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

A heterozygous missense variant in ACTG2 was identified that impaired actin polymerization in sporadic Megacystis microcolon intestinal hypoperistalsis syndrome.
Title: ACTG2 variants impair actin polymerization in sporadic Megacystis Microcolon Intestinal Hypoperistalsis Syndrome.

The following ACTG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27857	NM_001199893.2 Latest version!	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu27860	NM_001615.4 Latest version!	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu27860	NM_001615.3	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu27857	NM_001199893.1	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTG2 ( NM_001199893.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186822.1 Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27857
Clone ID Related Accession (Same CDS sequence)	NM_001199893.2 , NM_001199893.1
Accession Version	NM_001199893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	actin, gamma-enteric smooth muscle isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304523.1, X16940.1, BC012617.1 and AC073046.7. On May 31, 2019 this sequence version replaced NM_001199893.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, that results in a shorter isoform (2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304523.1, SRR7410570.204228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGATCT GGCACCACTC CTTCTACAAT GAGCTGCGTG TAGCACCTGA AGAGCACCCC 
ACCCTGCTCA CAGAGGCTCC CCTAAATCCC AAGGCCAACA GGGAAAAGAT GACCCAGATC 
ATGTTTGAAA CCTTCAATGT CCCTGCCATG TACGTCGCCA TTCAAGCTGT GCTCTCCCTC 
TATGCCTCTG GCCGCACGAC AGGCATCGTC CTGGATTCAG GTGATGGCGT CACCCACAAT 
GTCCCCATCT ATGAAGGCTA TGCCCTGCCC CATGCCATCA TGCGCCTGGA CTTGGCTGGC 
CGTGACCTCA CGGACTACCT CATGAAGATC CTCACAGAGA GAGGCTATTC CTTTGTGACC 
ACAGCTGAGA GAGAAATTGT GCGAGACATC AAGGAGAAGC TGTGCTATGT GGCCCTGGAT 
TTTGAGAATG AGATGGCCAC AGCAGCTTCC TCTTCCTCCC TGGAGAAGAG CTATGAGCTG 
CCAGATGGGC AGGTTATCAC CATTGGCAAT GAGCGCTTCC GCTGCCCTGA GACCCTCTTC 
CAGCCTTCCT TTATTGGCAT GGAGTCCGCT GGAATTCATG AGACAACCTA CAATTCCATC 
ATGAAGTGTG ACATTGACAT CCGTAAGGAC TTATATGCCA ACAATGTCCT CTCTGGGGGC 
ACCACCATGT ACCCTGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCCCCC 
AGCACCATGA AGATCAAGAT TATTGCTCCC CCAGAGCGGA AGTACTCAGT CTGGATCGGG 
GGCTCTATCC TGGCCTCTCT CTCCACCTTC CAGCAGATGT GGATCAGCAA GCCTGAGTAT 
GATGAGGCAG GGCCCTCCAT TGTCCACAGG AAGTGCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186822.1
CDS	81..1082
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001199893.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199893.2

ORF Insert Sequence:

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGATCT GGCACCACTC CTTCTACAAT GAGCTGCGTG TAGCACCTGA AGAGCACCCC 
ACCCTGCTCA CAGAGGCTCC CCTAAATCCC AAGGCCAACA GGGAAAAGAT GACCCAGATC 
ATGTTTGAAA CCTTCAATGT CCCTGCCATG TACGTCGCCA TTCAAGCTGT GCTCTCCCTC 
TATGCCTCTG GCCGCACGAC AGGCATCGTC CTGGATTCAG GTGATGGCGT CACCCACAAT 
GTCCCCATCT ATGAAGGCTA TGCCCTGCCC CATGCCATCA TGCGCCTGGA CTTGGCTGGC 
CGTGACCTCA CGGACTACCT CATGAAGATC CTCACAGAGA GAGGCTATTC CTTTGTGACC 
ACAGCTGAGA GAGAAATTGT GCGAGACATC AAGGAGAAGC TGTGCTATGT GGCCCTGGAT 
TTTGAGAATG AGATGGCCAC AGCAGCTTCC TCTTCCTCCC TGGAGAAGAG CTATGAGCTG 
CCAGATGGGC AGGTTATCAC CATTGGCAAT GAGCGCTTCC GCTGCCCTGA GACCCTCTTC 
CAGCCTTCCT TTATTGGCAT GGAGTCCGCT GGAATTCATG AGACAACCTA CAATTCCATC 
ATGAAGTGTG ACATTGACAT CCGTAAGGAC TTATATGCCA ACAATGTCCT CTCTGGGGGC 
ACCACCATGT ACCCTGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCCCCC 
AGCACCATGA AGATCAAGAT TATTGCTCCC CCAGAGCGGA AGTACTCAGT CTGGATCGGG 
GGCTCTATCC TGGCCTCTCT CTCCACCTTC CAGCAGATGT GGATCAGCAA GCCTGAGTAT 
GATGAGGCAG GGCCCTCCAT TGTCCACAGG AAGTGCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG2 ( NM_001199893.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186822.1 Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27857
Clone ID Related Accession (Same CDS sequence)	NM_001199893.2 , NM_001199893.1
Accession Version	NM_001199893.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Homo sapiens(human)
Product	actin, gamma-enteric smooth muscle isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI850192.1, AK304523.1, X16940.1 and BC012617.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, that results in a shorter isoform (2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304523.1, ERR279826.5295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGATCT GGCACCACTC CTTCTACAAT GAGCTGCGTG TAGCACCTGA AGAGCACCCC 
ACCCTGCTCA CAGAGGCTCC CCTAAATCCC AAGGCCAACA GGGAAAAGAT GACCCAGATC 
ATGTTTGAAA CCTTCAATGT CCCTGCCATG TACGTCGCCA TTCAAGCTGT GCTCTCCCTC 
TATGCCTCTG GCCGCACGAC AGGCATCGTC CTGGATTCAG GTGATGGCGT CACCCACAAT 
GTCCCCATCT ATGAAGGCTA TGCCCTGCCC CATGCCATCA TGCGCCTGGA CTTGGCTGGC 
CGTGACCTCA CGGACTACCT CATGAAGATC CTCACAGAGA GAGGCTATTC CTTTGTGACC 
ACAGCTGAGA GAGAAATTGT GCGAGACATC AAGGAGAAGC TGTGCTATGT GGCCCTGGAT 
TTTGAGAATG AGATGGCCAC AGCAGCTTCC TCTTCCTCCC TGGAGAAGAG CTATGAGCTG 
CCAGATGGGC AGGTTATCAC CATTGGCAAT GAGCGCTTCC GCTGCCCTGA GACCCTCTTC 
CAGCCTTCCT TTATTGGCAT GGAGTCCGCT GGAATTCATG AGACAACCTA CAATTCCATC 
ATGAAGTGTG ACATTGACAT CCGTAAGGAC TTATATGCCA ACAATGTCCT CTCTGGGGGC 
ACCACCATGT ACCCTGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCCCCC 
AGCACCATGA AGATCAAGAT TATTGCTCCC CCAGAGCGGA AGTACTCAGT CTGGATCGGG 
GGCTCTATCC TGGCCTCTCT CTCCACCTTC CAGCAGATGT GGATCAGCAA GCCTGAGTAT 
GATGAGGCAG GGCCCTCCAT TGTCCACAGG AAGTGCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186822.1
CDS	123..1124
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001199893.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199893.1

ORF Insert Sequence:

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGATCT GGCACCACTC CTTCTACAAT GAGCTGCGTG TAGCACCTGA AGAGCACCCC 
ACCCTGCTCA CAGAGGCTCC CCTAAATCCC AAGGCCAACA GGGAAAAGAT GACCCAGATC 
ATGTTTGAAA CCTTCAATGT CCCTGCCATG TACGTCGCCA TTCAAGCTGT GCTCTCCCTC 
TATGCCTCTG GCCGCACGAC AGGCATCGTC CTGGATTCAG GTGATGGCGT CACCCACAAT 
GTCCCCATCT ATGAAGGCTA TGCCCTGCCC CATGCCATCA TGCGCCTGGA CTTGGCTGGC 
CGTGACCTCA CGGACTACCT CATGAAGATC CTCACAGAGA GAGGCTATTC CTTTGTGACC 
ACAGCTGAGA GAGAAATTGT GCGAGACATC AAGGAGAAGC TGTGCTATGT GGCCCTGGAT 
TTTGAGAATG AGATGGCCAC AGCAGCTTCC TCTTCCTCCC TGGAGAAGAG CTATGAGCTG 
CCAGATGGGC AGGTTATCAC CATTGGCAAT GAGCGCTTCC GCTGCCCTGA GACCCTCTTC 
CAGCCTTCCT TTATTGGCAT GGAGTCCGCT GGAATTCATG AGACAACCTA CAATTCCATC 
ATGAAGTGTG ACATTGACAT CCGTAAGGAC TTATATGCCA ACAATGTCCT CTCTGGGGGC 
ACCACCATGT ACCCTGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCCCCC 
AGCACCATGA AGATCAAGAT TATTGCTCCC CCAGAGCGGA AGTACTCAGT CTGGATCGGG 
GGCTCTATCC TGGCCTCTCT CTCCACCTTC CAGCAGATGT GGATCAGCAA GCCTGAGTAT 
GATGAGGCAG GGCCCTCCAT TGTCCACAGG AAGTGCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG2 ( NM_001615.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001606.1 Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27860
Clone ID Related Accession (Same CDS sequence)	NM_001615.3 , NM_001615.4
Accession Version	NM_001615.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	actin, gamma-enteric smooth muscle isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI850192.1, X16940.1 and BC012617.1. This sequence is a reference standard in the RefSeqGene project. On May 5, 2005 this sequence version replaced NM_001615.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279842.5323.1, ERR279825.7880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGGGTG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA TGAGGCTCAG 
AGCAAGCGAG GGATCCTAAC TCTCAAATAC CCCATTGAAC ACGGCATCAT CACCAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAATG AGCTGCGTGT AGCACCTGAA 
GAGCACCCCA CCCTGCTCAC AGAGGCTCCC CTAAATCCCA AGGCCAACAG GGAAAAGATG 
ACCCAGATCA TGTTTGAAAC CTTCAATGTC CCTGCCATGT ACGTCGCCAT TCAAGCTGTG 
CTCTCCCTCT ATGCCTCTGG CCGCACGACA GGCATCGTCC TGGATTCAGG TGATGGCGTC 
ACCCACAATG TCCCCATCTA TGAAGGCTAT GCCCTGCCCC ATGCCATCAT GCGCCTGGAC 
TTGGCTGGCC GTGACCTCAC GGACTACCTC ATGAAGATCC TCACAGAGAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTATGTG 
GCCCTGGATT TTGAGAATGA GATGGCCACA GCAGCTTCCT CTTCCTCCCT GGAGAAGAGC 
TATGAGCTGC CAGATGGGCA GGTTATCACC ATTGGCAATG AGCGCTTCCG CTGCCCTGAG 
ACCCTCTTCC AGCCTTCCTT TATTGGCATG GAGTCCGCTG GAATTCATGA GACAACCTAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGTAAGGACT TATATGCCAA CAATGTCCTC 
TCTGGGGGCA CCACCATGTA CCCTGGCATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
CTGGCCCCCA GCACCATGAA GATCAAGATT ATTGCTCCCC CAGAGCGGAA GTACTCAGTC 
TGGATCGGGG GCTCTATCCT GGCCTCTCTC TCCACCTTCC AGCAGATGTG GATCAGCAAG 
CCTGAGTATG ATGAGGCAGG GCCCTCCATT GTCCACAGGA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001606.1
CDS	123..1253
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001615.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001615.3

ORF Insert Sequence:

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGGGTG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA TGAGGCTCAG 
AGCAAGCGAG GGATCCTAAC TCTCAAATAC CCCATTGAAC ACGGCATCAT CACCAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAATG AGCTGCGTGT AGCACCTGAA 
GAGCACCCCA CCCTGCTCAC AGAGGCTCCC CTAAATCCCA AGGCCAACAG GGAAAAGATG 
ACCCAGATCA TGTTTGAAAC CTTCAATGTC CCTGCCATGT ACGTCGCCAT TCAAGCTGTG 
CTCTCCCTCT ATGCCTCTGG CCGCACGACA GGCATCGTCC TGGATTCAGG TGATGGCGTC 
ACCCACAATG TCCCCATCTA TGAAGGCTAT GCCCTGCCCC ATGCCATCAT GCGCCTGGAC 
TTGGCTGGCC GTGACCTCAC GGACTACCTC ATGAAGATCC TCACAGAGAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTATGTG 
GCCCTGGATT TTGAGAATGA GATGGCCACA GCAGCTTCCT CTTCCTCCCT GGAGAAGAGC 
TATGAGCTGC CAGATGGGCA GGTTATCACC ATTGGCAATG AGCGCTTCCG CTGCCCTGAG 
ACCCTCTTCC AGCCTTCCTT TATTGGCATG GAGTCCGCTG GAATTCATGA GACAACCTAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGTAAGGACT TATATGCCAA CAATGTCCTC 
TCTGGGGGCA CCACCATGTA CCCTGGCATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
CTGGCCCCCA GCACCATGAA GATCAAGATT ATTGCTCCCC CAGAGCGGAA GTACTCAGTC 
TGGATCGGGG GCTCTATCCT GGCCTCTCTC TCCACCTTCC AGCAGATGTG GATCAGCAAG 
CCTGAGTATG ATGAGGCAGG GCCCTCCATT GTCCACAGGA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG2 ( NM_001615.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001606.1 Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27860
Clone ID Related Accession (Same CDS sequence)	NM_001615.3 , NM_001615.4
Accession Version	NM_001615.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	actin, gamma-enteric smooth muscle isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI850192.1, X16940.1, BC012617.1 and AC073046.7. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001615.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012617.1, AK312955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000345517.8/ ENSP00000295137.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGGGTG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA TGAGGCTCAG 
AGCAAGCGAG GGATCCTAAC TCTCAAATAC CCCATTGAAC ACGGCATCAT CACCAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAATG AGCTGCGTGT AGCACCTGAA 
GAGCACCCCA CCCTGCTCAC AGAGGCTCCC CTAAATCCCA AGGCCAACAG GGAAAAGATG 
ACCCAGATCA TGTTTGAAAC CTTCAATGTC CCTGCCATGT ACGTCGCCAT TCAAGCTGTG 
CTCTCCCTCT ATGCCTCTGG CCGCACGACA GGCATCGTCC TGGATTCAGG TGATGGCGTC 
ACCCACAATG TCCCCATCTA TGAAGGCTAT GCCCTGCCCC ATGCCATCAT GCGCCTGGAC 
TTGGCTGGCC GTGACCTCAC GGACTACCTC ATGAAGATCC TCACAGAGAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTATGTG 
GCCCTGGATT TTGAGAATGA GATGGCCACA GCAGCTTCCT CTTCCTCCCT GGAGAAGAGC 
TATGAGCTGC CAGATGGGCA GGTTATCACC ATTGGCAATG AGCGCTTCCG CTGCCCTGAG 
ACCCTCTTCC AGCCTTCCTT TATTGGCATG GAGTCCGCTG GAATTCATGA GACAACCTAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGTAAGGACT TATATGCCAA CAATGTCCTC 
TCTGGGGGCA CCACCATGTA CCCTGGCATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
CTGGCCCCCA GCACCATGAA GATCAAGATT ATTGCTCCCC CAGAGCGGAA GTACTCAGTC 
TGGATCGGGG GCTCTATCCT GGCCTCTCTC TCCACCTTCC AGCAGATGTG GATCAGCAAG 
CCTGAGTATG ATGAGGCAGG GCCCTCCATT GTCCACAGGA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001606.1
CDS	81..1211
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001615.4
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 2, smooth muscle (ACTG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001615.4

ORF Insert Sequence:

ATGTGTGAAG AGGAGACCAC CGCGCTCGTG TGTGACAATG GCTCTGGCCT GTGCAAGGCA 
GGCTTCGCAG GAGATGATGC CCCCCGGGCT GTCTTCCCCT CCATTGTGGG CCGCCCTCGC 
CACCAGGGTG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA TGAGGCTCAG 
AGCAAGCGAG GGATCCTAAC TCTCAAATAC CCCATTGAAC ACGGCATCAT CACCAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAATG AGCTGCGTGT AGCACCTGAA 
GAGCACCCCA CCCTGCTCAC AGAGGCTCCC CTAAATCCCA AGGCCAACAG GGAAAAGATG 
ACCCAGATCA TGTTTGAAAC CTTCAATGTC CCTGCCATGT ACGTCGCCAT TCAAGCTGTG 
CTCTCCCTCT ATGCCTCTGG CCGCACGACA GGCATCGTCC TGGATTCAGG TGATGGCGTC 
ACCCACAATG TCCCCATCTA TGAAGGCTAT GCCCTGCCCC ATGCCATCAT GCGCCTGGAC 
TTGGCTGGCC GTGACCTCAC GGACTACCTC ATGAAGATCC TCACAGAGAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTATGTG 
GCCCTGGATT TTGAGAATGA GATGGCCACA GCAGCTTCCT CTTCCTCCCT GGAGAAGAGC 
TATGAGCTGC CAGATGGGCA GGTTATCACC ATTGGCAATG AGCGCTTCCG CTGCCCTGAG 
ACCCTCTTCC AGCCTTCCTT TATTGGCATG GAGTCCGCTG GAATTCATGA GACAACCTAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGTAAGGACT TATATGCCAA CAATGTCCTC 
TCTGGGGGCA CCACCATGTA CCCTGGCATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
CTGGCCCCCA GCACCATGAA GATCAAGATT ATTGCTCCCC CAGAGCGGAA GTACTCAGTC 
TGGATCGGGG GCTCTATCCT GGCCTCTCTC TCCACCTTCC AGCAGATGTG GATCAGCAAG 
CCTGAGTATG ATGAGGCAGG GCCCTCCATT GTCCACAGGA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prenatal diagnosis of chronic intestinal pseudo-obstruction and paternal somatic mosaicism for the ACTG2 pathogenic variant.
Prenatal diagnosis37(12)1254-1256(2017 Dec)
Milunsky A,Lazier J,Baldwin C,Young C,Primack D,Milunsky JM

Diagnosis of Chronic Intestinal Pseudo-obstruction and Megacystis by Sequencing the ACTG2 Gene.
Journal of pediatric gastroenterology and nutrition65(4)384-387(2017 Oct)
Milunsky A,Baldwin C,Zhang X,Primack D,Curnow A,Milunsky J

Identification of ACTG2 functions as a promoter gene in hepatocellular carcinoma cells migration and tumor metastasis.
Biochemical and biophysical research communications491(2)537-544(2017 09)
Wu Y,Liu ZG,Shi MQ,Yu HZ,Jiang XY,Yang AH,Fu XS,Xu Y,Yang S,Ni H,Shen SJ,Li WD

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ACTG2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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