TOP2A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2A
Entrez Gene ID	7153
Full Name	DNA topoisomerase II alpha
Synonyms	TOP2,TP2A
General protein information	Preferred Names DNA topoisomerase II alpha Names DNA topoisomerase 2-alpha DNA gyrase DNA topoisomerase (ATP-hydrolyzing) DNA topoisomerase II, 170 kD DNA topoisomerase II, alpha isozyme topoisomerase (DNA) II alpha 170kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010].
Disorder MIM:	126430
Disorder Html:	DNA topoisomerase II, resistance to inhibition of, by amsacrine (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011525165.1	XP_011523467.1	DNA topoisomerase 2-alpha isoform X2
XM_005257632.1	XP_005257689.1	DNA topoisomerase 2-alpha isoform X1
NM_001067.3	NP_001058.2	DNA topoisomerase 2-alpha
NM_001067.4	NP_001058.2	DNA topoisomerase 2-alpha
XM_011525165.2	XP_011523467.1	DNA topoisomerase 2-alpha isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of Topoisomerase 2-Alpha Confers a Poor Prognosis in Pancreatic Adenocarcinoma Identified by Co-Expression Analysis.
Zhou Z, Liu S, Zhang M, Zhou R, Liu J, Chang Y, Zhao Q
Digestive diseases and sciences62(10)2790-2800(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings reveal a novel, p53-independent activity of Mdm2 and have important implications for the choice of chemotherapeutic agents in the treatment of Mdm2-overexpressing tumors. Herein is shown that tumor cells with MDM2 amplification are selectively resistant to treatment with topoisomerase II poisons but not other DNA damaging agents
Title: Mdm2 selectively suppresses DNA damage arising from inhibition of topoisomerase II independent of p53.

TOP2A was identified in association with the progression and prognosis of pancreatic ductal adenocarcinoma probably by regulating cell cycle and p53 signaling pathway.
Title: Overexpression of Topoisomerase 2-Alpha Confers a Poor Prognosis in Pancreatic Adenocarcinoma Identified by Co-Expression Analysis.

These results explain why hTOPIIa and hTOPIIa are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses.
Title: Natural variation in a single amino acid substitution underlies physiological responses to topoisomerase II poisons.

The decatenation checkpoint is regulated, not only by topo IIalpha, as previously reported, but also by topo IIbeta. The decatenation checkpoint is most efficient when both isoforms are present. Deletion of most of the C-terminus of topo IIalpha, while preserving the nuclear localization signal (NLS), enhances the decatenation checkpoint and sensitivity to topo II-targeted drugs. Mutation of Y640 in topo IIalpha inhibi...
Title: Roles of the C-terminal domains of topoisomerase II? and topoisomerase II? in regulation of the decatenation checkpoint.

Study found an association between TOP2alpha gene amplification and overexpression of its protein in patients with triple-negative breast cancer.
Title: Overexpression of p53 protein is a marker of poor prognosis in Mexican women with breast cancer.

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21029	NM_001067.4 Latest version!	Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu50627	XM_005257632.1 Latest version!	Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu61097	XM_011525165.2 Latest version!	Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu21029	NM_001067.3	Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu61097	XM_011525165.1	Homo sapiens topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2A ( NM_001067.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001058.2 Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.

Price & Availability↑

CloneID	OHu21029
Clone ID Related Accession (Same CDS sequence)	NM_001067.3 , NM_001067.4
Accession Version	NM_001067.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	DNA topoisomerase 2-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB069825.1, BC140791.1 and AC080112.15. This sequence is a reference standard in the RefSeqGene project. On Jul 7, 2010 this sequence version replaced NM_001067.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140791.1, J04088.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA 
GCTGATGATG TTAAGGGCAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA 
GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA 
GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA 
ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAAA AGCCTGATCC TGCCAAAACC 
AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA 
ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG 
GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG 
TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001058.2
CDS	160..4755
Translation	MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	NM_001067.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001067.3

ORF Insert Sequence:

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA 
GCTGATGATG TTAAGGGCAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA 
GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA 
GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA 
ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAAA AGCCTGATCC TGCCAAAACC 
AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA 
ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG 
GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG 
TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( NM_001067.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001058.2 Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.

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CloneID	OHu21029
Clone ID Related Accession (Same CDS sequence)	NM_001067.3 , NM_001067.4
Accession Version	NM_001067.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	DNA topoisomerase 2-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB069825.1, BC140791.1 and AC080112.15. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001067.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140791.1, J04088.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000423485.6/ ENSP00000411532.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA 
GCTGATGATG TTAAGGGCAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA 
GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA 
GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA 
ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAAA AGCCTGATCC TGCCAAAACC 
AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA 
ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG 
GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG 
TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001058.2
CDS	106..4701
Translation	MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	NM_001067.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001067.4

ORF Insert Sequence:

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA 
GCTGATGATG TTAAGGGCAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA 
GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA 
GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA 
ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAAA AGCCTGATCC TGCCAAAACC 
AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA 
ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG 
GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG 
TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_005257632.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257689.1 Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50627
Clone ID Related Accession (Same CDS sequence)	XM_005257632.1
Accession Version	XM_005257632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTCA ACAAAATAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TGTTGAAAGA 
ATCTATCAAA AGAAAACACA ATTGGAACAT ATTTTGCTCC GCCCAGACAC CTACATTGGT 
TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATGTTGG CATTAACTAT 
AGGGAAGTCA CTTTTGTTCC TGGTTTGTAC AAAATCTTTG ATGAGATTCT AGTTAATGCT 
GCGGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGATCCGGAA 
AACAATTTAA TTAGTATATG GAATAATGGA AAAGGTATTC CTGTTGTTGA ACACAAAGTT 
GAAAAGATGT ATGTCCCAGC TCTCATATTT GGACAGCTCC TAACTTCTAG TAACTATGAT 
GATGATGAAA AGAAAGTGAC AGGTGGTCGA AATGGCTATG GAGCCAAATT GTGTAACATA 
TTCAGTACCA AATTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG 
ACATGGATGG ATAATATGGG AAGAGCTGGT GAGATGGAAC TCAAGCCCTT CAATGGAGAA 
GATTATACAT GTATCACCTT TCAGCCTGAT TTGTCTAAGT TTAAAATGCA AAGCCTGGAC 
AAAGATATTG TTGCACTAAT GGTCAGAAGA GCATATGATA TTGCTGGATC CACCAAAGAT 
GTCAAAGTCT TTCTTAATGG AAATAAACTG CCAGTAAAAG GATTTCGTAG TTATGTGGAC 
ATGTATTTGA AGGACAAGTT GGATGAAACT GGTAACTCCT TGAAAGTAAT ACATGAACAA 
GTAAACCACA GGTGGGAAGT GTGTTTAACT ATGAGTGAAA AAGGCTTTCA GCAAATTAGC 
TTTGTCAACA GCATTGCTAC ATCCAAGGGT GGCAGACATG TTGATTATGT AGCTGATCAG 
ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA 
GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTAAATG CCTTAATTGA AAACCCAACC 
TTTGACTCTC AGACAAAAGA AAACATGACT TTACAACCCA AGAGCTTTGG ATCAACATGC 
CAATTGAGTG AAAAATTTAT CAAAGCTGCC ATTGGCTGTG GTATTGTAGA AAGCATACTA 
AACTGGGTGA AGTTTAAGGC CCAAGTCCAG TTAAACAAGA AGTGTTCAGC TGTAAAACAT 
AATAGAATCA AGGGAATTCC CAAACTCGAT GATGCCAATG ATGCAGGGGG CCGAAACTCC 
ACTGAGTGTA CGCTTATCCT GACTGAGGGA GATTCAGCCA AAACTTTGGC TGTTTCAGGC 
CTTGGTGTGG TTGGGAGAGA CAAATATGGG GTTTTCCCTC TTAGAGGAAA AATACTCAAT 
GTTCGAGAAG CTTCTCATAA GCAGATCATG GAAAATGCTG AGATTAACAA TATCATCAAG 
ATTGTGGGTC TTCAGTACAA GAAAAACTAT GAAGATGAAG ATTCATTGAA GACGCTTCGT 
TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCACAT CAAAGGCTTG 
CTGATTAATT TTATCCATCA CAACTGGCCC TCTCTTCTGC GACATCGTTT TCTGGAGGAA 
TTTATCACTC CCATTGTAAA GGTATCTAAA AACAAGCAAG AAATGGCATT TTACAGCCTT 
CCTGAATTTG AAGAGTGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAATAT 
TACAAAGGTT TGGGCACCAG CACATCAAAG GAAGCTAAAG AATACTTTGC AGATATGAAA 
AGACATCGTA TCCAGTTCAA ATATTCTGGT CCTGAAGATG ATGCTGCTAT CAGCCTGGCC 
TTTAGCAAAA AACAGATAGA TGATCGAAAG GAATGGTTAA CTAATTTCAT GGAGGATAGA 
AGACAACGAA AGTTACTTGG GCTTCCTGAG GATTACTTGT ATGGACAAAC TACCACATAT 
CTGACATATA ATGACTTCAT CAACAAGGAA CTTATCTTGT TCTCAAATTC TGATAACGAG 
AGATCTATCC CTTCTATGGT GGATGGTTTG AAACCAGGTC AGAGAAAGGT TTTGTTTACT 
TGCTTCAAAC GGAATGACAA GCGAGAAGTA AAGGTTGCCC AATTAGCTGG ATCAGTGGCT 
GAAATGTCTT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT CAATTTGGCT 
CAGAATTTTG TGGGTAGCAA TAATCTAAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACC 
AGGCTACATG GTGGCAAGGA TTCTGCTAGT CCACGATACA TCTTTACAAT GCTCAGCTCT 
TTGGCTCGAT TGTTATTTCC ACCAAAAGAT GATCACACGT TGAAGTTTTT ATATGATGAC 
AACCAGCGTG TTGAGCCTGA ATGGTACATT CCTATTATTC CCATGGTGCT GATAAATGGT 
GCTGAAGGAA TCGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATGT GCGTGAAATT 
GTAAATAACA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC 
AAGAACTTCA AGGGTACTAT TGAAGAACTG GCTCCAAATC AATATGTGAT TAGTGGTGAA 
GTAGCTATTC TTAATTCTAC AACCATTGAA ATCTCAGAGC TTCCCGTCAG AACATGGACC 
CAGACATACA AAGAACAAGT TCTAGAACCC ATGTTGAATG GCACCGAGAA GACACCTCCT 
CTCATAACAG ACTATAGGGA ATACCATACA GATACCACTG TGAAATTTGT TGTGAAGATG 
ACTGAAGAAA AACTGGCAGA GGCAGAGAGA GTTGGACTAC ACAAAGTCTT CAAACTCCAA 
ACTAGTCTCA CATGCAACTC TATGGTGCTT TTTGACCACG TAGGCTGTTT AAAGAAATAT 
GACACGGTGT TGGATATTCT AAGAGACTTT TTTGAACTCA GACTTAAATA TTATGGATTA 
AGAAAAGAAT GGCTCCTAGG AATGCTTGGT GCTGAATCTG CTAAACTGAA TAATCAGGCT 
CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATCATTG AAAATAAGCC TAAGAAAGAA 
TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC CTGGAAAGAA 
GCCCAGCAAA AGGTTCCAGA TGAAGAAGAA AATGAAGAGA GTGACAACGA AAAGGAAACT 
GAAAAGAGTG ACTCCGTAAC AGATTCTGGA CCAACCTTCA ACTATCTTCT TGATATGCCC 
CTTTGGTATT TAACCAAGGA AAAGAAAGAT GAACTCTGCA GGCTAAGAAA TGAAAAAGAA 
CAAGAGCTGG ACACATTAAA AAGAAAGAGT CCATCAGATT TGTGGAAAGA AGACTTGGCT 
ACATTTATTG AAGAATTGGA GGCTGTTGAA GCCAAGGAAA AACAAGATGA ACAAGTCGGA 
CTTCCTGGGA AAGGGGGGAA GGCCAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT 
TCTCCGCGTG GTCAAAGAGT CATTCCACGA ATAACCATAG AAATGAAAGC AGAGGCAGAA 
AAGAAAAATA AAAAGAAAAT TAAGAATGAA AATACTGAAG GAAGCCCTCA AGAAGATGGT 
GTGGAACTAG AAGGCCTAAA ACAAAGATTA GAAAAGAAAC AGAAAAGAGA ACCAGGTACA 
AAGACAAAGA AACAAACTAC ATTGGCATTT AAGCCAATCA AAAAAGGAAA GAAGAGAAAT 
CCCTGGTCTG ATTCAGAATC AGATAGGAGC AGTGACGAAA GTAATTTTGA TGTCCCTCCA 
CGAGAAACAG AGCCACGGAG AGCAGCAACA AAAACAAAAT TCACAATGGA TTTGGATTCA 
GATGAAGATT TCTCAGATTT TGATGAAAAA ACTGATGATG AAGATTTTGT CCCATCAGAT 
GCTAGTCCAC CTAAGACCAA AACTTCCCCA AAACTTAGTA ACAAAGAACT GAAACCACAG 
AAAAGTGTCG TGTCAGACCT TGAAGCTGAT GATGTTAAGG GCAGTGTACC ACTGTCTTCA 
AGCCCTCCTG CTACACATTT CCCAGATGAA ACTGAAATTA CAAACCCAGT TCCTAAAAAG 
AATGTGACAG TGAAGAAGAC AGCAGCAAAA AGTCAGTCTT CCACCTCCAC TACCGGTGCC 
AAAAAAAGGG CTGCCCCAAA AGGAACTAAA AGGGATCCAG CTTTGAATTC TGGTGTCTCT 
CAAAAGCCTG ATCCTGCCAA AACCAAGAAT CGCCGCAAAA GGAAGCCATC CACTTCTGAT 
GATTCTGACT CTAATTTTGA GAAAATTGTT TCGAAAGCAG TCACAAGCAA GAAATCCAAG 
GGGGAGAGTG ATGACTTCCA TATGGACTTT GACTCAGCTG TGGCTCCTCG GGCAAAATCT 
GTACGGGCAA AGAAACCTAT AAAGTACCTG GAAGAGTCAG ATGAAGATGA TCTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257689.1
CDS	138..4697
Translation	MQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_005257632.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257632.1

ORF Insert Sequence:

ATGCAAGTCA ACAAAATAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TGTTGAAAGA 
ATCTATCAAA AGAAAACACA ATTGGAACAT ATTTTGCTCC GCCCAGACAC CTACATTGGT 
TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATGTTGG CATTAACTAT 
AGGGAAGTCA CTTTTGTTCC TGGTTTGTAC AAAATCTTTG ATGAGATTCT AGTTAATGCT 
GCGGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGATCCGGAA 
AACAATTTAA TTAGTATATG GAATAATGGA AAAGGTATTC CTGTTGTTGA ACACAAAGTT 
GAAAAGATGT ATGTCCCAGC TCTCATATTT GGACAGCTCC TAACTTCTAG TAACTATGAT 
GATGATGAAA AGAAAGTGAC AGGTGGTCGA AATGGCTATG GAGCCAAATT GTGTAACATA 
TTCAGTACCA AATTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG 
ACATGGATGG ATAATATGGG AAGAGCTGGT GAGATGGAAC TCAAGCCCTT CAATGGAGAA 
GATTATACAT GTATCACCTT TCAGCCTGAT TTGTCTAAGT TTAAAATGCA AAGCCTGGAC 
AAAGATATTG TTGCACTAAT GGTCAGAAGA GCATATGATA TTGCTGGATC CACCAAAGAT 
GTCAAAGTCT TTCTTAATGG AAATAAACTG CCAGTAAAAG GATTTCGTAG TTATGTGGAC 
ATGTATTTGA AGGACAAGTT GGATGAAACT GGTAACTCCT TGAAAGTAAT ACATGAACAA 
GTAAACCACA GGTGGGAAGT GTGTTTAACT ATGAGTGAAA AAGGCTTTCA GCAAATTAGC 
TTTGTCAACA GCATTGCTAC ATCCAAGGGT GGCAGACATG TTGATTATGT AGCTGATCAG 
ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA 
GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTAAATG CCTTAATTGA AAACCCAACC 
TTTGACTCTC AGACAAAAGA AAACATGACT TTACAACCCA AGAGCTTTGG ATCAACATGC 
CAATTGAGTG AAAAATTTAT CAAAGCTGCC ATTGGCTGTG GTATTGTAGA AAGCATACTA 
AACTGGGTGA AGTTTAAGGC CCAAGTCCAG TTAAACAAGA AGTGTTCAGC TGTAAAACAT 
AATAGAATCA AGGGAATTCC CAAACTCGAT GATGCCAATG ATGCAGGGGG CCGAAACTCC 
ACTGAGTGTA CGCTTATCCT GACTGAGGGA GATTCAGCCA AAACTTTGGC TGTTTCAGGC 
CTTGGTGTGG TTGGGAGAGA CAAATATGGG GTTTTCCCTC TTAGAGGAAA AATACTCAAT 
GTTCGAGAAG CTTCTCATAA GCAGATCATG GAAAATGCTG AGATTAACAA TATCATCAAG 
ATTGTGGGTC TTCAGTACAA GAAAAACTAT GAAGATGAAG ATTCATTGAA GACGCTTCGT 
TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCACAT CAAAGGCTTG 
CTGATTAATT TTATCCATCA CAACTGGCCC TCTCTTCTGC GACATCGTTT TCTGGAGGAA 
TTTATCACTC CCATTGTAAA GGTATCTAAA AACAAGCAAG AAATGGCATT TTACAGCCTT 
CCTGAATTTG AAGAGTGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAATAT 
TACAAAGGTT TGGGCACCAG CACATCAAAG GAAGCTAAAG AATACTTTGC AGATATGAAA 
AGACATCGTA TCCAGTTCAA ATATTCTGGT CCTGAAGATG ATGCTGCTAT CAGCCTGGCC 
TTTAGCAAAA AACAGATAGA TGATCGAAAG GAATGGTTAA CTAATTTCAT GGAGGATAGA 
AGACAACGAA AGTTACTTGG GCTTCCTGAG GATTACTTGT ATGGACAAAC TACCACATAT 
CTGACATATA ATGACTTCAT CAACAAGGAA CTTATCTTGT TCTCAAATTC TGATAACGAG 
AGATCTATCC CTTCTATGGT GGATGGTTTG AAACCAGGTC AGAGAAAGGT TTTGTTTACT 
TGCTTCAAAC GGAATGACAA GCGAGAAGTA AAGGTTGCCC AATTAGCTGG ATCAGTGGCT 
GAAATGTCTT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT CAATTTGGCT 
CAGAATTTTG TGGGTAGCAA TAATCTAAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACC 
AGGCTACATG GTGGCAAGGA TTCTGCTAGT CCACGATACA TCTTTACAAT GCTCAGCTCT 
TTGGCTCGAT TGTTATTTCC ACCAAAAGAT GATCACACGT TGAAGTTTTT ATATGATGAC 
AACCAGCGTG TTGAGCCTGA ATGGTACATT CCTATTATTC CCATGGTGCT GATAAATGGT 
GCTGAAGGAA TCGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATGT GCGTGAAATT 
GTAAATAACA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC 
AAGAACTTCA AGGGTACTAT TGAAGAACTG GCTCCAAATC AATATGTGAT TAGTGGTGAA 
GTAGCTATTC TTAATTCTAC AACCATTGAA ATCTCAGAGC TTCCCGTCAG AACATGGACC 
CAGACATACA AAGAACAAGT TCTAGAACCC ATGTTGAATG GCACCGAGAA GACACCTCCT 
CTCATAACAG ACTATAGGGA ATACCATACA GATACCACTG TGAAATTTGT TGTGAAGATG 
ACTGAAGAAA AACTGGCAGA GGCAGAGAGA GTTGGACTAC ACAAAGTCTT CAAACTCCAA 
ACTAGTCTCA CATGCAACTC TATGGTGCTT TTTGACCACG TAGGCTGTTT AAAGAAATAT 
GACACGGTGT TGGATATTCT AAGAGACTTT TTTGAACTCA GACTTAAATA TTATGGATTA 
AGAAAAGAAT GGCTCCTAGG AATGCTTGGT GCTGAATCTG CTAAACTGAA TAATCAGGCT 
CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATCATTG AAAATAAGCC TAAGAAAGAA 
TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC CTGGAAAGAA 
GCCCAGCAAA AGGTTCCAGA TGAAGAAGAA AATGAAGAGA GTGACAACGA AAAGGAAACT 
GAAAAGAGTG ACTCCGTAAC AGATTCTGGA CCAACCTTCA ACTATCTTCT TGATATGCCC 
CTTTGGTATT TAACCAAGGA AAAGAAAGAT GAACTCTGCA GGCTAAGAAA TGAAAAAGAA 
CAAGAGCTGG ACACATTAAA AAGAAAGAGT CCATCAGATT TGTGGAAAGA AGACTTGGCT 
ACATTTATTG AAGAATTGGA GGCTGTTGAA GCCAAGGAAA AACAAGATGA ACAAGTCGGA 
CTTCCTGGGA AAGGGGGGAA GGCCAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT 
TCTCCGCGTG GTCAAAGAGT CATTCCACGA ATAACCATAG AAATGAAAGC AGAGGCAGAA 
AAGAAAAATA AAAAGAAAAT TAAGAATGAA AATACTGAAG GAAGCCCTCA AGAAGATGGT 
GTGGAACTAG AAGGCCTAAA ACAAAGATTA GAAAAGAAAC AGAAAAGAGA ACCAGGTACA 
AAGACAAAGA AACAAACTAC ATTGGCATTT AAGCCAATCA AAAAAGGAAA GAAGAGAAAT 
CCCTGGTCTG ATTCAGAATC AGATAGGAGC AGTGACGAAA GTAATTTTGA TGTCCCTCCA 
CGAGAAACAG AGCCACGGAG AGCAGCAACA AAAACAAAAT TCACAATGGA TTTGGATTCA 
GATGAAGATT TCTCAGATTT TGATGAAAAA ACTGATGATG AAGATTTTGT CCCATCAGAT 
GCTAGTCCAC CTAAGACCAA AACTTCCCCA AAACTTAGTA ACAAAGAACT GAAACCACAG 
AAAAGTGTCG TGTCAGACCT TGAAGCTGAT GATGTTAAGG GCAGTGTACC ACTGTCTTCA 
AGCCCTCCTG CTACACATTT CCCAGATGAA ACTGAAATTA CAAACCCAGT TCCTAAAAAG 
AATGTGACAG TGAAGAAGAC AGCAGCAAAA AGTCAGTCTT CCACCTCCAC TACCGGTGCC 
AAAAAAAGGG CTGCCCCAAA AGGAACTAAA AGGGATCCAG CTTTGAATTC TGGTGTCTCT 
CAAAAGCCTG ATCCTGCCAA AACCAAGAAT CGCCGCAAAA GGAAGCCATC CACTTCTGAT 
GATTCTGACT CTAATTTTGA GAAAATTGTT TCGAAAGCAG TCACAAGCAA GAAATCCAAG 
GGGGAGAGTG ATGACTTCCA TATGGACTTT GACTCAGCTG TGGCTCCTCG GGCAAAATCT 
GTACGGGCAA AGAAACCTAT AAAGTACCTG GAAGAGTCAG ATGAAGATGA TCTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_011525165.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523467.1 Homo sapiens topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

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CloneID	OHu61097
Clone ID Related Accession (Same CDS sequence)	XM_011525165.1 , XM_011525165.2
Accession Version	XM_011525165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523467.1
CDS	106..4200
Translation	MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKP

Target ORF information:

RefSeq Version	XM_011525165.1
Organism	Homo sapiens(human)
Definition	Homo sapiens topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525165.1

ORF Insert Sequence:

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_011525165.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523467.1 Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA.

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CloneID	OHu61097
Clone ID Related Accession (Same CDS sequence)	XM_011525165.1 , XM_011525165.2
Accession Version	XM_011525165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523467.1
CDS	112..4206
Translation	MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKP

Target ORF information:

RefSeq Version	XM_011525165.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525165.2

ORF Insert Sequence:

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG 
GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC 
ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA 
GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC 
AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT 
AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT 
GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT 
TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC 
ATGACTTTAC AACCCAAGAG CTTTGGATCA ACATGCCAAT TGAGTGAAAA ATTTATCAAA 
GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA 
CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT 
GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG 
ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACG CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTA 
TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA GTGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT 
TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT 
CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC 
AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA 
GAAGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT 
GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA 
AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CGTCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA 
GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC 
CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA 
GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT 
TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG 
AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT 
GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CGCGTGGTCA AAGAGTCATT 
CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG 
GCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT 
AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA 
GCAACAAAAA CAAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT 
GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT 
TCCCCAAAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of Topoisomerase 2-Alpha Confers a Poor Prognosis in Pancreatic Adenocarcinoma Identified by Co-Expression Analysis.
Digestive diseases and sciences62(10)2790-2800(2017 10)
Zhou Z,Liu S,Zhang M,Zhou R,Liu J,Chang Y,Zhao Q

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TOP2A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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