TNNC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNNC1
Entrez Gene ID	7134
Full Name	troponin C1, slow skeletal and cardiac type
Synonyms	CMD1Z,CMH13,TN-C,TNC,TNNC
General protein information	Preferred Names troponin C1, slow skeletal and cardiac type Names troponin C, slow skeletal and cardiac muscles cardiac troponin C slow twitch skeletal/cardiac muscle troponin C troponin C type 1 (slow)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.1
Summary	Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008].
Disorder MIM:	191040
Disorder Html:	Cardiomyopathy, dilated, 1Z, 611879 (3); Cardiomyopathy, hypertrophic, 13, 613243 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003280.3	NP_003271.1	troponin C, slow skeletal and cardiac muscles
NM_003280.2	NP_003271.1	troponin C, slow skeletal and cardiac muscles

Pathways from BioSystems

Homologies

	LOC697047	XP_001085656.2
Canis lupus familiaris (dog)	TNNC1	XP_005632514.1
Homo sapiens (human)	TNNC1	NP_003271.1
Xenopus tropicalis (tropical clawed frog)	tnnc1	NP_001004776.1
Bos taurus (cattle)	TNNC1	NP_001029523.1
Rattus norvegicus (Norway rat)	Tnnc1	NP_001029277.1
Gallus gallus (chicken)	TNNC1	NP_990464.1
Pan troglodytes (chimpanzee)	TNNC1	XP_003950175.1
Mus musculus (house mouse)	Tnnc1	NP_033419.1
Danio rerio (zebrafish)	tnnc1a	NP_852475.2

Gene Ontology
Bibliography

Related articles in PubMed

Changes in the dynamics of the cardiac troponin C molecule explain the effects of Ca
Stevens CM, Rayani K, Singh G, Lotfalisalmasi B, Tieleman DP, Tibbits GF
The Journal of biological chemistry292(28)11915-11926(2017 07)

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with ?14 TnT.
Baxley T, Johnson D, Pinto JR, Chalovich JM
Biochemistry56(23)2928-2937(2017 06)

Use of the HEART Pathway with high sensitivity cardiac troponins: A secondary analysis.
Mahler SA, Stopyra JP, Apple FS, Riley RF, Russell GB, Hiestand BC, Hoekstra JW, Lefebvre CW, Nicks BA, Cline DM, Askew KL, Herrington DM, Burke GL, Miller CD
Clinical biochemistry50(7-8)401-407(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that modulation of structural dynamics far from the regulatory Ca2+-binding site is the underlying molecular mechanism for many TNNC1 mutations in patients with hypertrophic cardiomyopathies or familial hypertrophic cardiomyopathies; many mutations affect balance between open and closed conformations; troponin I switch peptide [TnI(SW)] switch peptide binds to TNNC1 and stabilizes the open TNNC1 conformation.
Title: Changes in the dynamics of the cardiac troponin C molecule explain the effects of Ca

Data suggest that mutations in troponin C (TnC) found in patients with hypertrophic cardiomyopathy (A8V, C84Y, and D145E) stabilize the active state of regulated actin (the actin-tropomyosin-troponin complex) to various extents; at a saturating Ca2+ concentration, all TnC mutants investigated increase the level of active M state compared to the wild type.
Title: Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with ?14 TnT.

There was no difference in the test characteristics of the HEART Pathway whether using cTnI or hs-cTnI, with both achieving 100% sensitivity and NPV. Use of hs-cTnT with the HEART Pathway was associated with one missed major adverse cardiac events.
Title: Use of the HEART Pathway with high sensitivity cardiac troponins: A secondary analysis.

Our results (i) confirm that genetic backgrounds of hypertrophic cardiomyopathy and restrictive cardiomyopathy overlap and (ii) indicate that TNNC1 is a likely novel gene for autosomal recessive restrictive cardiomyopathy.
Title: Evidence for troponin C (TNNC1) as a gene for autosomal recessive restrictive cardiomyopathy with fatal outcome in infancy.

contractility is constantly above normal in hearts made hypertrophic by TnC with the A8V mutation
Title: Enhanced troponin I binding explains the functional changes produced by the hypertrophic cardiomyopathy mutation A8V of cardiac troponin C.

The following TNNC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26822	NM_003280.3 Latest version!	Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26822	NM_003280.2	Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNNC1 ( NM_003280.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003271.1 Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.

Price & Availability↑

CloneID	OHu26822
Clone ID Related Accession (Same CDS sequence)	NM_003280.3 , NM_003280.2
Accession Version	NM_003280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	troponin C, slow skeletal and cardiac muscles
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X07897.1 and AJ574625.1. On May 17, 2019 this sequence version replaced NM_003280.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.140968.1, SRR5189655.222916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000232975.8/ ENSP00000232975.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATGACA TCTACAAGGC TGCGGTAGAG CAGCTGACAG AAGAGCAGAA AAATGAGTTC 
AAGGCAGCCT TCGACATCTT CGTGCTGGGC GCTGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGCCAG AACCCCACCC CTGAGGAGCT GCAGGAGATG 
ATCGATGAGG TGGACGAGGA CGGCAGCGGC ACGGTGGACT TTGATGAGTT CCTGGTCATG 
ATGGTTCGGT GCATGAAGGA CGACAGCAAA GGGAAATCTG AGGAGGAGCT GTCTGACCTC 
TTCCGCATGT TTGACAAAAA TGCTGATGGC TACATCGACC TGGATGAGCT GAAGATAATG 
CTGCAGGCTA CAGGCGAGAC CATCACGGAG GACGACATCG AGGAGCTCAT GAAGGACGGA 
GACAAGAACA ACGACGGCCG CATCGACTAT GATGAGTTCC TGGAGTTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003271.1
CDS	27..512
Translation	MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

Target ORF information:

RefSeq Version	NM_003280.3
Organism	Homo sapiens(human)
Definition	Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003280.3

ORF Insert Sequence:

ATGGATGACA TCTACAAGGC TGCGGTAGAG CAGCTGACAG AAGAGCAGAA AAATGAGTTC 
AAGGCAGCCT TCGACATCTT CGTGCTGGGC GCTGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGCCAG AACCCCACCC CTGAGGAGCT GCAGGAGATG 
ATCGATGAGG TGGACGAGGA CGGCAGCGGC ACGGTGGACT TTGATGAGTT CCTGGTCATG 
ATGGTTCGGT GCATGAAGGA CGACAGCAAA GGGAAATCTG AGGAGGAGCT GTCTGACCTC 
TTCCGCATGT TTGACAAAAA TGCTGATGGC TACATCGACC TGGATGAGCT GAAGATAATG 
CTGCAGGCTA CAGGCGAGAC CATCACGGAG GACGACATCG AGGAGCTCAT GAAGGACGGA 
GACAAGAACA ACGACGGCCG CATCGACTAT GATGAGTTCC TGGAGTTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNC1 ( NM_003280.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003271.1 Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.

Price & Availability↑

CloneID	OHu26822
Clone ID Related Accession (Same CDS sequence)	NM_003280.3 , NM_003280.2
Accession Version	NM_003280.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	troponin C, slow skeletal and cardiac muscles
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X07897.1 and AJ574625.1. This sequence is a reference standard in the RefSeqGene project. On Sep 6, 2008 this sequence version replaced NM_003280.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279844.6703.1, ERR279851.2917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACA TCTACAAGGC TGCGGTAGAG CAGCTGACAG AAGAGCAGAA AAATGAGTTC 
AAGGCAGCCT TCGACATCTT CGTGCTGGGC GCTGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGCCAG AACCCCACCC CTGAGGAGCT GCAGGAGATG 
ATCGATGAGG TGGACGAGGA CGGCAGCGGC ACGGTGGACT TTGATGAGTT CCTGGTCATG 
ATGGTTCGGT GCATGAAGGA CGACAGCAAA GGGAAATCTG AGGAGGAGCT GTCTGACCTC 
TTCCGCATGT TTGACAAAAA TGCTGATGGC TACATCGACC TGGATGAGCT GAAGATAATG 
CTGCAGGCTA CAGGCGAGAC CATCACGGAG GACGACATCG AGGAGCTCAT GAAGGACGGA 
GACAAGAACA ACGACGGCCG CATCGACTAT GATGAGTTCC TGGAGTTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003271.1
CDS	27..512
Translation	MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

Target ORF information:

RefSeq Version	NM_003280.2
Organism	Homo sapiens(human)
Definition	Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003280.2

ORF Insert Sequence:

ATGGATGACA TCTACAAGGC TGCGGTAGAG CAGCTGACAG AAGAGCAGAA AAATGAGTTC 
AAGGCAGCCT TCGACATCTT CGTGCTGGGC GCTGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGCCAG AACCCCACCC CTGAGGAGCT GCAGGAGATG 
ATCGATGAGG TGGACGAGGA CGGCAGCGGC ACGGTGGACT TTGATGAGTT CCTGGTCATG 
ATGGTTCGGT GCATGAAGGA CGACAGCAAA GGGAAATCTG AGGAGGAGCT GTCTGACCTC 
TTCCGCATGT TTGACAAAAA TGCTGATGGC TACATCGACC TGGATGAGCT GAAGATAATG 
CTGCAGGCTA CAGGCGAGAC CATCACGGAG GACGACATCG AGGAGCTCAT GAAGGACGGA 
GACAAGAACA ACGACGGCCG CATCGACTAT GATGAGTTCC TGGAGTTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Changes in the dynamics of the cardiac troponin C molecule explain the effects of Ca
The Journal of biological chemistry292(28)11915-11926(2017 07)
Stevens CM,Rayani K,Singh G,Lotfalisalmasi B,Tieleman DP,Tibbits GF

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with ?14 TnT.
Biochemistry56(23)2928-2937(2017 06)
Baxley T,Johnson D,Pinto JR,Chalovich JM

Use of the HEART Pathway with high sensitivity cardiac troponins: A secondary analysis.
Clinical biochemistry50(7-8)401-407(2017 May)
Mahler SA,Stopyra JP,Apple FS,Riley RF,Russell GB,Hiestand BC,Hoekstra JW,Lefebvre CW,Nicks BA,Cline DM,Askew KL,Herrington DM,Burke GL,Miller CD

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TNNC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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