TIE1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017002207.1	XP_016857696.1	tyrosine-protein kinase receptor Tie-1 isoform X2
XM_006710869.1	XP_006710932.1	tyrosine-protein kinase receptor Tie-1 isoform X3
XM_005271163.2	XP_005271220.1	tyrosine-protein kinase receptor Tie-1 isoform X1
NM_005424.5	NP_005415.1	tyrosine-protein kinase receptor Tie-1 isoform 1 precursor
NM_001253357.2	NP_001240286.1	tyrosine-protein kinase receptor Tie-1 isoform 2
XM_017002207.2	XP_016857696.1	tyrosine-protein kinase receptor Tie-1 isoform X2
NM_001253357.1	NP_001240286.1	tyrosine-protein kinase receptor Tie-1 isoform 2
NM_005424.4	NP_005415.1	tyrosine-protein kinase receptor Tie-1 isoform 1 precursor
XM_005271163.1	XP_005271220.1	tyrosine-protein kinase receptor Tie-1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu33752	XM_017002207.2 Latest version!	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu33753	XM_006710869.1 Latest version!	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu19829	NM_005424.5 Latest version!	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu20207	NM_001253357.2 Latest version!	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu33751	XM_005271163.2 Latest version!	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu33752	XM_017002207.1	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu20207	NM_001253357.1	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu19829	NM_005424.4	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu33751	XM_005271163.1	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

TIE1 ( XM_017002207.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857696.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu33752
Clone ID Related Accession (Same CDS sequence)	XM_017002207.1 , XM_017002207.2
Accession Version	XM_017002207.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG CCTTCCTGGG CTCGGGAGCC AGCCTCTGTC TGCACACATC TTTGGCTGAT 
GTTCTTAGCC CTGCTGTGGC CACCTGCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857696.1
CDS	121..2613
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSAFLGSGASLCLHTSLADVLSPAVATCQ

Target ORF information:

RefSeq Version	XM_017002207.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002207.1

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG CCTTCCTGGG CTCGGGAGCC AGCCTCTGTC TGCACACATC TTTGGCTGAT 
GTTCTTAGCC CTGCTGTGGC CACCTGCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_017002207.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857696.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu33752
Clone ID Related Accession (Same CDS sequence)	XM_017002207.1 , XM_017002207.2
Accession Version	XM_017002207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG CCTTCCTGGG CTCGGGAGCC AGCCTCTGTC TGCACACATC TTTGGCTGAT 
GTTCTTAGCC CTGCTGTGGC CACCTGCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857696.1
CDS	119..2611
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSAFLGSGASLCLHTSLADVLSPAVATCQ

Target ORF information:

RefSeq Version	XM_017002207.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002207.2

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG CCTTCCTGGG CTCGGGAGCC AGCCTCTGTC TGCACACATC TTTGGCTGAT 
GTTCTTAGCC CTGCTGTGGC CACCTGCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_006710869.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710932.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X4, mRNA.

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CloneID	OHu33753
Clone ID Related Accession (Same CDS sequence)	XM_006710869.1
Accession Version	XM_006710869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AACTGGAGTT CAACTTAGAG ACGATGCCCC GGATCAACTG TGCAGCTGCA 
GGGAACCCCT TCCCCGTGCG GGGCAGCATA GAGCTACGCA AGCCAGACGG CACTGTGCTC 
CTGTCCACCA AGGCCATTGT GGAGCCAGAG AAGACCACAG CTGAGTTCGA GGTGCCCCGC 
TTGGTTCTTG CGGACAGTGG GTTCTGGGAG TGCCGTGTGT CCACATCTGG CGGCCAAGAC 
AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC 
CTGACCAAGC AGAGCCGCCA GCTTGTGGTC TCCCCGCTGG TCTCGTTCTC TGGGGATGGA 
CCCATCTCCA CTGTCCGCCT GCACTACCGG CCCCAGGACA GTACCATGGA CTGGTCGACC 
ATTGTGGTGG ACCCCAGTGA GAACGTGACG TTAATGAACC TGAGGCCAAA GACAGGATAC 
AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC 
ACCCTCATGA CCACAGACTG TCCTGAGCCT TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT 
GTGGAAGGCA CTGACCGGCT GCGAGTGAGC TGGTCCTTGC CCTTGGTGCC CGGGCCACTG 
GTGGGCGACG GTTTCCTGCT GCGCCTGTGG GACGGGACAC GGGGGCAGGA GCGGCGGGAG 
AACGTCTCAT CCCCCCAGGC CCGCACTGCC CTCCTGACGG GACTCACGCC TGGCACCCAC 
TACCAGCTGG ATGTGCAGCT CTACCACTGC ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA 
CACGTGCTTC TGCCCCCCAG TGGGCCTCCA GCCCCCCGAC ACCTCCACGC CCAGGCCCTC 
TCAGACTCCG AGATCCAGCT GACATGGAAG CACCCGGAGG CTCTGCCTGG GCCAATATCC 
AAGTACGTTG TGGAGGTGCA GGTGGCTGGG GGTGCAGGAG ACCCACTGTG GATAGACGTG 
GACAGGCCTG AGGAGACAAG CACCATCATC CGTGGCCTCA ACGCCAGCAC GCGCTACCTC 
TTCCGCATGC GGGCCAGCAT TCAGGGGCTC GGGGACTGGA GCAACACAGT AGAAGAGTCC 
ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG 
GGCCTGGATC AGCAGCTGAT CCTGGCGGTG GTGGGCTCCG TGTCTGCCAC CTGCCTCACC 
ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC ATCCGCAGAA GCTGCCTGCA TCGGAGACGC 
ACCTTCACCT ACCAGTCAGG CTCGGGCGAG GAGACCATCC TGCAGTTCAG CTCAGGGACC 
TTGACACTTA CCCGGCGGCC AAAACTGCAG CCCGAGCCCC TGAGCTACCC AGTGCTAGAG 
TGGGAGGACA TCACCTTTGA GGACCTCATC GGGGAGGGGA ACTTCGGCCA GGTCATCCGG 
GCCATGATCA AGAAGGACGG GCTGAAGATG AACGCAGCCA TCAAAATGCT GAAAGAGTAT 
GCCTCTGAAA ATGACCATCG TGACTTTGCG GGAGAACTGG AAGTTCTGTG CAAATTGGGG 
CATCACCCCA ACATCATCAA CCTCCTGGGG GCCTGTAAGA ACCGAGGTTA CTTGTATATC 
GCTATTGAAT ATGCCCCCTA CGGGAACCTG CTAGATTTTC TGCGGAAAAG CCGGGTCCTA 
GAGACTGACC CAGCTTTTGC TCGAGAGCAT GGGACAGCCT CTACCCTTAG CTCCCGGCAG 
CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT GGCATGCAGT ACCTGAGTGA GAAGCAGTTC 
ATCCACAGGG ACCTGGCTGC CCGGAATGTG CTGGTCGGAG AGAACCTAGC CTCCAAGATT 
GCAGACTTCG GCCTTTCTCG GGGAGAGGAG GTTTATGTGA AGAAGACGAT GGGGCGTCTC 
CCTGTGCGCT GGATGGCCAT TGAGTCCCTG AACTACAGTG TCTATACCAC CAAGAGTGAT 
GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG ATAGTGAGCC TTGGAGGTAC ACCCTACTGT 
GGCATGACCT GTGCCGAGCT CTATGAAAAG CTGCCCCAGG GCTACCGCAT GGAGCAGCCT 
CGAAACTGTG ACGATGAAGT GTACGAGCTG ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT 
GAGCGACCCC CCTTTGCCCA GATTGCGCTA CAGCTAGGCC GCATGCTGGA AGCCAGGAAG 
GCCTATGTGA ACATGTCGCT GTTTGAGAAC TTCACTTACG CGGGCATTGA TGCCACAGCT 
GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710932.1
CDS	1377..3728
Translation	MASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_006710869.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710869.1

ORF Insert Sequence:

ATGGCCTCAG AACTGGAGTT CAACTTAGAG ACGATGCCCC GGATCAACTG TGCAGCTGCA 
GGGAACCCCT TCCCCGTGCG GGGCAGCATA GAGCTACGCA AGCCAGACGG CACTGTGCTC 
CTGTCCACCA AGGCCATTGT GGAGCCAGAG AAGACCACAG CTGAGTTCGA GGTGCCCCGC 
TTGGTTCTTG CGGACAGTGG GTTCTGGGAG TGCCGTGTGT CCACATCTGG CGGCCAAGAC 
AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC 
CTGACCAAGC AGAGCCGCCA GCTTGTGGTC TCCCCGCTGG TCTCGTTCTC TGGGGATGGA 
CCCATCTCCA CTGTCCGCCT GCACTACCGG CCCCAGGACA GTACCATGGA CTGGTCGACC 
ATTGTGGTGG ACCCCAGTGA GAACGTGACG TTAATGAACC TGAGGCCAAA GACAGGATAC 
AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC 
ACCCTCATGA CCACAGACTG TCCTGAGCCT TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT 
GTGGAAGGCA CTGACCGGCT GCGAGTGAGC TGGTCCTTGC CCTTGGTGCC CGGGCCACTG 
GTGGGCGACG GTTTCCTGCT GCGCCTGTGG GACGGGACAC GGGGGCAGGA GCGGCGGGAG 
AACGTCTCAT CCCCCCAGGC CCGCACTGCC CTCCTGACGG GACTCACGCC TGGCACCCAC 
TACCAGCTGG ATGTGCAGCT CTACCACTGC ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA 
CACGTGCTTC TGCCCCCCAG TGGGCCTCCA GCCCCCCGAC ACCTCCACGC CCAGGCCCTC 
TCAGACTCCG AGATCCAGCT GACATGGAAG CACCCGGAGG CTCTGCCTGG GCCAATATCC 
AAGTACGTTG TGGAGGTGCA GGTGGCTGGG GGTGCAGGAG ACCCACTGTG GATAGACGTG 
GACAGGCCTG AGGAGACAAG CACCATCATC CGTGGCCTCA ACGCCAGCAC GCGCTACCTC 
TTCCGCATGC GGGCCAGCAT TCAGGGGCTC GGGGACTGGA GCAACACAGT AGAAGAGTCC 
ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG 
GGCCTGGATC AGCAGCTGAT CCTGGCGGTG GTGGGCTCCG TGTCTGCCAC CTGCCTCACC 
ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC ATCCGCAGAA GCTGCCTGCA TCGGAGACGC 
ACCTTCACCT ACCAGTCAGG CTCGGGCGAG GAGACCATCC TGCAGTTCAG CTCAGGGACC 
TTGACACTTA CCCGGCGGCC AAAACTGCAG CCCGAGCCCC TGAGCTACCC AGTGCTAGAG 
TGGGAGGACA TCACCTTTGA GGACCTCATC GGGGAGGGGA ACTTCGGCCA GGTCATCCGG 
GCCATGATCA AGAAGGACGG GCTGAAGATG AACGCAGCCA TCAAAATGCT GAAAGAGTAT 
GCCTCTGAAA ATGACCATCG TGACTTTGCG GGAGAACTGG AAGTTCTGTG CAAATTGGGG 
CATCACCCCA ACATCATCAA CCTCCTGGGG GCCTGTAAGA ACCGAGGTTA CTTGTATATC 
GCTATTGAAT ATGCCCCCTA CGGGAACCTG CTAGATTTTC TGCGGAAAAG CCGGGTCCTA 
GAGACTGACC CAGCTTTTGC TCGAGAGCAT GGGACAGCCT CTACCCTTAG CTCCCGGCAG 
CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT GGCATGCAGT ACCTGAGTGA GAAGCAGTTC 
ATCCACAGGG ACCTGGCTGC CCGGAATGTG CTGGTCGGAG AGAACCTAGC CTCCAAGATT 
GCAGACTTCG GCCTTTCTCG GGGAGAGGAG GTTTATGTGA AGAAGACGAT GGGGCGTCTC 
CCTGTGCGCT GGATGGCCAT TGAGTCCCTG AACTACAGTG TCTATACCAC CAAGAGTGAT 
GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG ATAGTGAGCC TTGGAGGTAC ACCCTACTGT 
GGCATGACCT GTGCCGAGCT CTATGAAAAG CTGCCCCAGG GCTACCGCAT GGAGCAGCCT 
CGAAACTGTG ACGATGAAGT GTACGAGCTG ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT 
GAGCGACCCC CCTTTGCCCA GATTGCGCTA CAGCTAGGCC GCATGCTGGA AGCCAGGAAG 
GCCTATGTGA ACATGTCGCT GTTTGAGAAC TTCACTTACG CGGGCATTGA TGCCACAGCT 
GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( NM_005424.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005415.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.

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CloneID	OHu19829
Clone ID Related Accession (Same CDS sequence)	NM_005424.5 , NM_005424.4
Accession Version	NM_005424.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB287371.1, BC038239.1 and R13130.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005424.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038239.1, X60957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372476.8/ ENSP00000361554.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG GCGAGGAGAC CATCCTGCAG TTCAGCTCAG GGACCTTGAC ACTTACCCGG 
CGGCCAAAAC TGCAGCCCGA GCCCCTGAGC TACCCAGTGC TAGAGTGGGA GGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTC GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTGA AGATGAACGC AGCCATCAAA ATGCTGAAAG AGTATGCCTC TGAAAATGAC 
CATCGTGACT TTGCGGGAGA ACTGGAAGTT CTGTGCAAAT TGGGGCATCA CCCCAACATC 
ATCAACCTCC TGGGGGCCTG TAAGAACCGA GGTTACTTGT ATATCGCTAT TGAATATGCC 
CCCTACGGGA ACCTGCTAGA TTTTCTGCGG AAAAGCCGGG TCCTAGAGAC TGACCCAGCT 
TTTGCTCGAG AGCATGGGAC AGCCTCTACC CTTAGCTCCC GGCAGCTGCT GCGTTTCGCC 
AGTGATGCGG CCAATGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGGGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTAGCCTCCA AGATTGCAGA CTTCGGCCTT 
TCTCGGGGAG AGGAGGTTTA TGTGAAGAAG ACGATGGGGC GTCTCCCTGT GCGCTGGATG 
GCCATTGAGT CCCTGAACTA CAGTGTCTAT ACCACCAAGA GTGATGTCTG GTCCTTTGGA 
GTCCTTCTTT GGGAGATAGT GAGCCTTGGA GGTACACCCT ACTGTGGCAT GACCTGTGCC 
GAGCTCTATG AAAAGCTGCC CCAGGGCTAC CGCATGGAGC AGCCTCGAAA CTGTGACGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CCTATGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTACAGCT AGGCCGCATG CTGGAAGCCA GGAAGGCCTA TGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATTGATGCCA CAGCTGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005415.1
CDS	91..3507
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_005424.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005424.5

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG GCGAGGAGAC CATCCTGCAG TTCAGCTCAG GGACCTTGAC ACTTACCCGG 
CGGCCAAAAC TGCAGCCCGA GCCCCTGAGC TACCCAGTGC TAGAGTGGGA GGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTC GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTGA AGATGAACGC AGCCATCAAA ATGCTGAAAG AGTATGCCTC TGAAAATGAC 
CATCGTGACT TTGCGGGAGA ACTGGAAGTT CTGTGCAAAT TGGGGCATCA CCCCAACATC 
ATCAACCTCC TGGGGGCCTG TAAGAACCGA GGTTACTTGT ATATCGCTAT TGAATATGCC 
CCCTACGGGA ACCTGCTAGA TTTTCTGCGG AAAAGCCGGG TCCTAGAGAC TGACCCAGCT 
TTTGCTCGAG AGCATGGGAC AGCCTCTACC CTTAGCTCCC GGCAGCTGCT GCGTTTCGCC 
AGTGATGCGG CCAATGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGGGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTAGCCTCCA AGATTGCAGA CTTCGGCCTT 
TCTCGGGGAG AGGAGGTTTA TGTGAAGAAG ACGATGGGGC GTCTCCCTGT GCGCTGGATG 
GCCATTGAGT CCCTGAACTA CAGTGTCTAT ACCACCAAGA GTGATGTCTG GTCCTTTGGA 
GTCCTTCTTT GGGAGATAGT GAGCCTTGGA GGTACACCCT ACTGTGGCAT GACCTGTGCC 
GAGCTCTATG AAAAGCTGCC CCAGGGCTAC CGCATGGAGC AGCCTCGAAA CTGTGACGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CCTATGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTACAGCT AGGCCGCATG CTGGAAGCCA GGAAGGCCTA TGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATTGATGCCA CAGCTGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( NM_005424.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005415.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.

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CloneID	OHu19829
Clone ID Related Accession (Same CDS sequence)	NM_005424.5 , NM_005424.4
Accession Version	NM_005424.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB287371.1, BC038239.1, R13130.1 and BM970991.1. This sequence is a reference standard in the RefSeqGene project. On Nov 29, 2011 this sequence version replaced NM_005424.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038239.1, X60957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG GCGAGGAGAC CATCCTGCAG TTCAGCTCAG GGACCTTGAC ACTTACCCGG 
CGGCCAAAAC TGCAGCCCGA GCCCCTGAGC TACCCAGTGC TAGAGTGGGA GGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTC GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTGA AGATGAACGC AGCCATCAAA ATGCTGAAAG AGTATGCCTC TGAAAATGAC 
CATCGTGACT TTGCGGGAGA ACTGGAAGTT CTGTGCAAAT TGGGGCATCA CCCCAACATC 
ATCAACCTCC TGGGGGCCTG TAAGAACCGA GGTTACTTGT ATATCGCTAT TGAATATGCC 
CCCTACGGGA ACCTGCTAGA TTTTCTGCGG AAAAGCCGGG TCCTAGAGAC TGACCCAGCT 
TTTGCTCGAG AGCATGGGAC AGCCTCTACC CTTAGCTCCC GGCAGCTGCT GCGTTTCGCC 
AGTGATGCGG CCAATGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGGGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTAGCCTCCA AGATTGCAGA CTTCGGCCTT 
TCTCGGGGAG AGGAGGTTTA TGTGAAGAAG ACGATGGGGC GTCTCCCTGT GCGCTGGATG 
GCCATTGAGT CCCTGAACTA CAGTGTCTAT ACCACCAAGA GTGATGTCTG GTCCTTTGGA 
GTCCTTCTTT GGGAGATAGT GAGCCTTGGA GGTACACCCT ACTGTGGCAT GACCTGTGCC 
GAGCTCTATG AAAAGCTGCC CCAGGGCTAC CGCATGGAGC AGCCTCGAAA CTGTGACGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CCTATGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTACAGCT AGGCCGCATG CTGGAAGCCA GGAAGGCCTA TGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATTGATGCCA CAGCTGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005415.1
CDS	178..3594
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_005424.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005424.4

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGCTTGTGC CCCTGGTCAT TTTGGGGCTG ATTGCCGACT CCAGTGCCAG 
TGTCAGAATG GTGGCACTTG TGACCGGTTC AGTGGTTGTG TCTGCCCCTC TGGGTGGCAT 
GGAGTGCACT GTGAGAAGTC AGACCGGATC CCCCAGATCC TCAACATGGC CTCAGAACTG 
GAGTTCAACT TAGAGACGAT GCCCCGGATC AACTGTGCAG CTGCAGGGAA CCCCTTCCCC 
GTGCGGGGCA GCATAGAGCT ACGCAAGCCA GACGGCACTG TGCTCCTGTC CACCAAGGCC 
ATTGTGGAGC CAGAGAAGAC CACAGCTGAG TTCGAGGTGC CCCGCTTGGT TCTTGCGGAC 
AGTGGGTTCT GGGAGTGCCG TGTGTCCACA TCTGGCGGCC AAGACAGCCG GCGCTTCAAG 
GTCAATGTGA AAGTGCCCCC CGTGCCCCTG GCTGCACCTC GGCTCCTGAC CAAGCAGAGC 
CGCCAGCTTG TGGTCTCCCC GCTGGTCTCG TTCTCTGGGG ATGGACCCAT CTCCACTGTC 
CGCCTGCACT ACCGGCCCCA GGACAGTACC ATGGACTGGT CGACCATTGT GGTGGACCCC 
AGTGAGAACG TGACGTTAAT GAACCTGAGG CCAAAGACAG GATACAGTGT TCGTGTGCAG 
CTGAGCCGGC CAGGGGAAGG AGGAGAGGGG GCCTGGGGGC CTCCCACCCT CATGACCACA 
GACTGTCCTG AGCCTTTGTT GCAGCCGTGG TTGGAGGGCT GGCATGTGGA AGGCACTGAC 
CGGCTGCGAG TGAGCTGGTC CTTGCCCTTG GTGCCCGGGC CACTGGTGGG CGACGGTTTC 
CTGCTGCGCC TGTGGGACGG GACACGGGGG CAGGAGCGGC GGGAGAACGT CTCATCCCCC 
CAGGCCCGCA CTGCCCTCCT GACGGGACTC ACGCCTGGCA CCCACTACCA GCTGGATGTG 
CAGCTCTACC ACTGCACCCT CCTGGGCCCG GCCTCGCCCC CTGCACACGT GCTTCTGCCC 
CCCAGTGGGC CTCCAGCCCC CCGACACCTC CACGCCCAGG CCCTCTCAGA CTCCGAGATC 
CAGCTGACAT GGAAGCACCC GGAGGCTCTG CCTGGGCCAA TATCCAAGTA CGTTGTGGAG 
GTGCAGGTGG CTGGGGGTGC AGGAGACCCA CTGTGGATAG ACGTGGACAG GCCTGAGGAG 
ACAAGCACCA TCATCCGTGG CCTCAACGCC AGCACGCGCT ACCTCTTCCG CATGCGGGCC 
AGCATTCAGG GGCTCGGGGA CTGGAGCAAC ACAGTAGAAG AGTCCACCCT GGGCAACGGG 
CTGCAGGCTG AGGGCCCAGT CCAAGAGAGC CGGGCAGCTG AAGAGGGCCT GGATCAGCAG 
CTGATCCTGG CGGTGGTGGG CTCCGTGTCT GCCACCTGCC TCACCATCCT GGCTGCCCTT 
TTAACCCTGG TGTGCATCCG CAGAAGCTGC CTGCATCGGA GACGCACCTT CACCTACCAG 
TCAGGCTCGG GCGAGGAGAC CATCCTGCAG TTCAGCTCAG GGACCTTGAC ACTTACCCGG 
CGGCCAAAAC TGCAGCCCGA GCCCCTGAGC TACCCAGTGC TAGAGTGGGA GGACATCACC 
TTTGAGGACC TCATCGGGGA GGGGAACTTC GGCCAGGTCA TCCGGGCCAT GATCAAGAAG 
GACGGGCTGA AGATGAACGC AGCCATCAAA ATGCTGAAAG AGTATGCCTC TGAAAATGAC 
CATCGTGACT TTGCGGGAGA ACTGGAAGTT CTGTGCAAAT TGGGGCATCA CCCCAACATC 
ATCAACCTCC TGGGGGCCTG TAAGAACCGA GGTTACTTGT ATATCGCTAT TGAATATGCC 
CCCTACGGGA ACCTGCTAGA TTTTCTGCGG AAAAGCCGGG TCCTAGAGAC TGACCCAGCT 
TTTGCTCGAG AGCATGGGAC AGCCTCTACC CTTAGCTCCC GGCAGCTGCT GCGTTTCGCC 
AGTGATGCGG CCAATGGCAT GCAGTACCTG AGTGAGAAGC AGTTCATCCA CAGGGACCTG 
GCTGCCCGGA ATGTGCTGGT CGGAGAGAAC CTAGCCTCCA AGATTGCAGA CTTCGGCCTT 
TCTCGGGGAG AGGAGGTTTA TGTGAAGAAG ACGATGGGGC GTCTCCCTGT GCGCTGGATG 
GCCATTGAGT CCCTGAACTA CAGTGTCTAT ACCACCAAGA GTGATGTCTG GTCCTTTGGA 
GTCCTTCTTT GGGAGATAGT GAGCCTTGGA GGTACACCCT ACTGTGGCAT GACCTGTGCC 
GAGCTCTATG AAAAGCTGCC CCAGGGCTAC CGCATGGAGC AGCCTCGAAA CTGTGACGAT 
GAAGTGTACG AGCTGATGCG TCAGTGCTGG CGGGACCGTC CCTATGAGCG ACCCCCCTTT 
GCCCAGATTG CGCTACAGCT AGGCCGCATG CTGGAAGCCA GGAAGGCCTA TGTGAACATG 
TCGCTGTTTG AGAACTTCAC TTACGCGGGC ATTGATGCCA CAGCTGAGGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( NM_001253357.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240286.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20207
Clone ID Related Accession (Same CDS sequence)	NM_001253357.2 , NM_001253357.1
Accession Version	NM_001253357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB287371.1, AK300399.1, R13130.1 and BM970991.1. On May 31, 2019 this sequence version replaced NM_001253357.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300399.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCG GGGCGGGGAG GGGCTCGGAC GCCTGGGGCC CGCCCCTGCT GCTGGAGAAG 
GACGACCGTA TCGTGCGCAC CCCGCCCGGG CCACCCCTGC GCCTGGCGCG CAACGGTTCG 
CACCAGGTCA CGCTTCGCGG CTTCTCCAAG CCCTCGGACC TCGTGGGCGT CTTCTCCTGC 
GTGGGCGGTG CTGGGGCGCG GCGCACGCGC GTCATCTACG TGCACAACAG CCCTGGAGCC 
CACCTGCTTC CAGACAAGGT CACACACACT GTGAACAAAG GTGACACCGC TGTACTTTCT 
GCACGTGTGC ACAAGGAGAA GCAGACAGAC GTGATCTGGA AGAGCAACGG ATCCTACTTC 
TACACCCTGG ACTGGCATGA AGCCCAGGAT GGGCGGTTCC TGCTGCAGCT CCCAAATGTG 
CAGCCACCAT CGAGCGGCAT CTACAGTGCC ACTTACCTGG AAGCCAGCCC CCTGGGCAGC 
GCCTTCTTTC GGCTCATCGT GCGGGGTTGT GGGGCTGGGC GCTGGGGGCC AGGCTGTACC 
AAGGAGTGCC CAGGTTGCCT ACATGGAGGT GTCTGCCACG ACCATGACGG CGAATGTGTA 
TGCCCCCCTG GCTTCACTGG CACCCGCTGT GAACAGGCCT GCAGAGAGGG CCGTTTTGGG 
CAGAGCTGCC AGGAGCAGTG CCCAGGCATA TCAGGCTGCC GGGGCCTCAC CTTCTGCCTC 
CCAGACCCCT ATGGCTGCTC TTGTGGATCT GGCTGGAGAG GAAGCCAGTG CCAAGAAGCT 
TGTGCCCCTG GTCATTTTGG GGCTGATTGC CGACTCCAGT GCCAGTGTCA GAATGGTGGC 
ACTTGTGACC GGTTCAGTGG TTGTGTCTGC CCCTCTGGGT GGCATGGAGT GCACTGTGAG 
AAGTCAGACC GGATCCCCCA GATCCTCAAC ATGGCCTCAG AACTGGAGTT CAACTTAGAG 
ACGATGCCCC GGATCAACTG TGCAGCTGCA GGGAACCCCT TCCCCGTGCG GGGCAGCATA 
GAGCTACGCA AGCCAGACGG CACTGTGCTC CTGTCCACCA AGGCCATTGT GGAGCCAGAG 
AAGACCACAG CTGAGTTCGA GGTGCCCCGC TTGGTTCTTG CGGACAGTGG GTTCTGGGAG 
TGCCGTGTGT CCACATCTGG CGGCCAAGAC AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG 
CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC CTGACCAAGC AGAGCCGCCA GCTTGTGGTC 
TCCCCGCTGG TCTCGTTCTC TGGGGATGGA CCCATCTCCA CTGTCCGCCT GCACTACCGG 
CCCCAGGACA GTACCATGGA CTGGTCGACC ATTGTGGTGG ACCCCAGTGA GAACGTGACG 
TTAATGAACC TGAGGCCAAA GACAGGATAC AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG 
GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC ACCCTCATGA CCACAGACTG TCCTGAGCCT 
TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT GTGGAAGGCA CTGACCGGCT GCGAGTGAGC 
TGGTCCTTGC CCTTGGTGCC CGGGCCACTG GTGGGCGACG GTTTCCTGCT GCGCCTGTGG 
GACGGGACAC GGGGGCAGGA GCGGCGGGAG AACGTCTCAT CCCCCCAGGC CCGCACTGCC 
CTCCTGACGG GACTCACGCC TGGCACCCAC TACCAGCTGG ATGTGCAGCT CTACCACTGC 
ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA CACGTGCTTC TGCCCCCCAG TGGGCCTCCA 
GCCCCCCGAC ACCTCCACGC CCAGGCCCTC TCAGACTCCG AGATCCAGCT GACATGGAAG 
CACCCGGAGG CTCTGCCTGG GCCAATATCC AAGTACGTTG TGGAGGTGCA GGTGGCTGGG 
GGTGCAGGAG ACCCACTGTG GATAGACGTG GACAGGCCTG AGGAGACAAG CACCATCATC 
CGTGGCCTCA ACGCCAGCAC GCGCTACCTC TTCCGCATGC GGGCCAGCAT TCAGGGGCTC 
GGGGACTGGA GCAACACAGT AGAAGAGTCC ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC 
CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG GGCCTGGATC AGCAGCTGAT CCTGGCGGTG 
GTGGGCTCCG TGTCTGCCAC CTGCCTCACC ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC 
ATCCGCAGAA GCTGCCTGCA TCGGAGACGC ACCTTCACCT ACCAGTCAGG CTCGGGCGAG 
GAGACCATCC TGCAGTTCAG CTCAGGGACC TTGACACTTA CCCGGCGGCC AAAACTGCAG 
CCCGAGCCCC TGAGCTACCC AGTGCTAGAG TGGGAGGACA TCACCTTTGA GGACCTCATC 
GGGGAGGGGA ACTTCGGCCA GGTCATCCGG GCCATGATCA AGAAGGACGG GCTGAAGATG 
AACGCAGCCA TCAAAATGCT GAAAGAGTAT GCCTCTGAAA ATGACCATCG TGACTTTGCG 
GGAGAACTGG AAGTTCTGTG CAAATTGGGG CATCACCCCA ACATCATCAA CCTCCTGGGG 
GCCTGTAAGA ACCGAGGTTA CTTGTATATC GCTATTGAAT ATGCCCCCTA CGGGAACCTG 
CTAGATTTTC TGCGGAAAAG CCGGGTCCTA GAGACTGACC CAGCTTTTGC TCGAGAGCAT 
GGGACAGCCT CTACCCTTAG CTCCCGGCAG CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT 
GGCATGCAGT ACCTGAGTGA GAAGCAGTTC ATCCACAGGG ACCTGGCTGC CCGGAATGTG 
CTGGTCGGAG AGAACCTAGC CTCCAAGATT GCAGACTTCG GCCTTTCTCG GGGAGAGGAG 
GTTTATGTGA AGAAGACGAT GGGGCGTCTC CCTGTGCGCT GGATGGCCAT TGAGTCCCTG 
AACTACAGTG TCTATACCAC CAAGAGTGAT GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG 
ATAGTGAGCC TTGGAGGTAC ACCCTACTGT GGCATGACCT GTGCCGAGCT CTATGAAAAG 
CTGCCCCAGG GCTACCGCAT GGAGCAGCCT CGAAACTGTG ACGATGAAGT GTACGAGCTG 
ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT GAGCGACCCC CCTTTGCCCA GATTGCGCTA 
CAGCTAGGCC GCATGCTGGA AGCCAGGAAG GCCTATGTGA ACATGTCGCT GTTTGAGAAC 
TTCACTTACG CGGGCATTGA TGCCACAGCT GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240286.1
CDS	143..3424
Translation	MWAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_001253357.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253357.2

ORF Insert Sequence:

ATGTGGGCCG GGGCGGGGAG GGGCTCGGAC GCCTGGGGCC CGCCCCTGCT GCTGGAGAAG 
GACGACCGTA TCGTGCGCAC CCCGCCCGGG CCACCCCTGC GCCTGGCGCG CAACGGTTCG 
CACCAGGTCA CGCTTCGCGG CTTCTCCAAG CCCTCGGACC TCGTGGGCGT CTTCTCCTGC 
GTGGGCGGTG CTGGGGCGCG GCGCACGCGC GTCATCTACG TGCACAACAG CCCTGGAGCC 
CACCTGCTTC CAGACAAGGT CACACACACT GTGAACAAAG GTGACACCGC TGTACTTTCT 
GCACGTGTGC ACAAGGAGAA GCAGACAGAC GTGATCTGGA AGAGCAACGG ATCCTACTTC 
TACACCCTGG ACTGGCATGA AGCCCAGGAT GGGCGGTTCC TGCTGCAGCT CCCAAATGTG 
CAGCCACCAT CGAGCGGCAT CTACAGTGCC ACTTACCTGG AAGCCAGCCC CCTGGGCAGC 
GCCTTCTTTC GGCTCATCGT GCGGGGTTGT GGGGCTGGGC GCTGGGGGCC AGGCTGTACC 
AAGGAGTGCC CAGGTTGCCT ACATGGAGGT GTCTGCCACG ACCATGACGG CGAATGTGTA 
TGCCCCCCTG GCTTCACTGG CACCCGCTGT GAACAGGCCT GCAGAGAGGG CCGTTTTGGG 
CAGAGCTGCC AGGAGCAGTG CCCAGGCATA TCAGGCTGCC GGGGCCTCAC CTTCTGCCTC 
CCAGACCCCT ATGGCTGCTC TTGTGGATCT GGCTGGAGAG GAAGCCAGTG CCAAGAAGCT 
TGTGCCCCTG GTCATTTTGG GGCTGATTGC CGACTCCAGT GCCAGTGTCA GAATGGTGGC 
ACTTGTGACC GGTTCAGTGG TTGTGTCTGC CCCTCTGGGT GGCATGGAGT GCACTGTGAG 
AAGTCAGACC GGATCCCCCA GATCCTCAAC ATGGCCTCAG AACTGGAGTT CAACTTAGAG 
ACGATGCCCC GGATCAACTG TGCAGCTGCA GGGAACCCCT TCCCCGTGCG GGGCAGCATA 
GAGCTACGCA AGCCAGACGG CACTGTGCTC CTGTCCACCA AGGCCATTGT GGAGCCAGAG 
AAGACCACAG CTGAGTTCGA GGTGCCCCGC TTGGTTCTTG CGGACAGTGG GTTCTGGGAG 
TGCCGTGTGT CCACATCTGG CGGCCAAGAC AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG 
CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC CTGACCAAGC AGAGCCGCCA GCTTGTGGTC 
TCCCCGCTGG TCTCGTTCTC TGGGGATGGA CCCATCTCCA CTGTCCGCCT GCACTACCGG 
CCCCAGGACA GTACCATGGA CTGGTCGACC ATTGTGGTGG ACCCCAGTGA GAACGTGACG 
TTAATGAACC TGAGGCCAAA GACAGGATAC AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG 
GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC ACCCTCATGA CCACAGACTG TCCTGAGCCT 
TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT GTGGAAGGCA CTGACCGGCT GCGAGTGAGC 
TGGTCCTTGC CCTTGGTGCC CGGGCCACTG GTGGGCGACG GTTTCCTGCT GCGCCTGTGG 
GACGGGACAC GGGGGCAGGA GCGGCGGGAG AACGTCTCAT CCCCCCAGGC CCGCACTGCC 
CTCCTGACGG GACTCACGCC TGGCACCCAC TACCAGCTGG ATGTGCAGCT CTACCACTGC 
ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA CACGTGCTTC TGCCCCCCAG TGGGCCTCCA 
GCCCCCCGAC ACCTCCACGC CCAGGCCCTC TCAGACTCCG AGATCCAGCT GACATGGAAG 
CACCCGGAGG CTCTGCCTGG GCCAATATCC AAGTACGTTG TGGAGGTGCA GGTGGCTGGG 
GGTGCAGGAG ACCCACTGTG GATAGACGTG GACAGGCCTG AGGAGACAAG CACCATCATC 
CGTGGCCTCA ACGCCAGCAC GCGCTACCTC TTCCGCATGC GGGCCAGCAT TCAGGGGCTC 
GGGGACTGGA GCAACACAGT AGAAGAGTCC ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC 
CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG GGCCTGGATC AGCAGCTGAT CCTGGCGGTG 
GTGGGCTCCG TGTCTGCCAC CTGCCTCACC ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC 
ATCCGCAGAA GCTGCCTGCA TCGGAGACGC ACCTTCACCT ACCAGTCAGG CTCGGGCGAG 
GAGACCATCC TGCAGTTCAG CTCAGGGACC TTGACACTTA CCCGGCGGCC AAAACTGCAG 
CCCGAGCCCC TGAGCTACCC AGTGCTAGAG TGGGAGGACA TCACCTTTGA GGACCTCATC 
GGGGAGGGGA ACTTCGGCCA GGTCATCCGG GCCATGATCA AGAAGGACGG GCTGAAGATG 
AACGCAGCCA TCAAAATGCT GAAAGAGTAT GCCTCTGAAA ATGACCATCG TGACTTTGCG 
GGAGAACTGG AAGTTCTGTG CAAATTGGGG CATCACCCCA ACATCATCAA CCTCCTGGGG 
GCCTGTAAGA ACCGAGGTTA CTTGTATATC GCTATTGAAT ATGCCCCCTA CGGGAACCTG 
CTAGATTTTC TGCGGAAAAG CCGGGTCCTA GAGACTGACC CAGCTTTTGC TCGAGAGCAT 
GGGACAGCCT CTACCCTTAG CTCCCGGCAG CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT 
GGCATGCAGT ACCTGAGTGA GAAGCAGTTC ATCCACAGGG ACCTGGCTGC CCGGAATGTG 
CTGGTCGGAG AGAACCTAGC CTCCAAGATT GCAGACTTCG GCCTTTCTCG GGGAGAGGAG 
GTTTATGTGA AGAAGACGAT GGGGCGTCTC CCTGTGCGCT GGATGGCCAT TGAGTCCCTG 
AACTACAGTG TCTATACCAC CAAGAGTGAT GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG 
ATAGTGAGCC TTGGAGGTAC ACCCTACTGT GGCATGACCT GTGCCGAGCT CTATGAAAAG 
CTGCCCCAGG GCTACCGCAT GGAGCAGCCT CGAAACTGTG ACGATGAAGT GTACGAGCTG 
ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT GAGCGACCCC CCTTTGCCCA GATTGCGCTA 
CAGCTAGGCC GCATGCTGGA AGCCAGGAAG GCCTATGTGA ACATGTCGCT GTTTGAGAAC 
TTCACTTACG CGGGCATTGA TGCCACAGCT GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( NM_001253357.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240286.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.

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CloneID	OHu20207
Clone ID Related Accession (Same CDS sequence)	NM_001253357.2 , NM_001253357.1
Accession Version	NM_001253357.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB287371.1, AK300399.1, R13130.1 and BM970991.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300399.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCG GGGCGGGGAG GGGCTCGGAC GCCTGGGGCC CGCCCCTGCT GCTGGAGAAG 
GACGACCGTA TCGTGCGCAC CCCGCCCGGG CCACCCCTGC GCCTGGCGCG CAACGGTTCG 
CACCAGGTCA CGCTTCGCGG CTTCTCCAAG CCCTCGGACC TCGTGGGCGT CTTCTCCTGC 
GTGGGCGGTG CTGGGGCGCG GCGCACGCGC GTCATCTACG TGCACAACAG CCCTGGAGCC 
CACCTGCTTC CAGACAAGGT CACACACACT GTGAACAAAG GTGACACCGC TGTACTTTCT 
GCACGTGTGC ACAAGGAGAA GCAGACAGAC GTGATCTGGA AGAGCAACGG ATCCTACTTC 
TACACCCTGG ACTGGCATGA AGCCCAGGAT GGGCGGTTCC TGCTGCAGCT CCCAAATGTG 
CAGCCACCAT CGAGCGGCAT CTACAGTGCC ACTTACCTGG AAGCCAGCCC CCTGGGCAGC 
GCCTTCTTTC GGCTCATCGT GCGGGGTTGT GGGGCTGGGC GCTGGGGGCC AGGCTGTACC 
AAGGAGTGCC CAGGTTGCCT ACATGGAGGT GTCTGCCACG ACCATGACGG CGAATGTGTA 
TGCCCCCCTG GCTTCACTGG CACCCGCTGT GAACAGGCCT GCAGAGAGGG CCGTTTTGGG 
CAGAGCTGCC AGGAGCAGTG CCCAGGCATA TCAGGCTGCC GGGGCCTCAC CTTCTGCCTC 
CCAGACCCCT ATGGCTGCTC TTGTGGATCT GGCTGGAGAG GAAGCCAGTG CCAAGAAGCT 
TGTGCCCCTG GTCATTTTGG GGCTGATTGC CGACTCCAGT GCCAGTGTCA GAATGGTGGC 
ACTTGTGACC GGTTCAGTGG TTGTGTCTGC CCCTCTGGGT GGCATGGAGT GCACTGTGAG 
AAGTCAGACC GGATCCCCCA GATCCTCAAC ATGGCCTCAG AACTGGAGTT CAACTTAGAG 
ACGATGCCCC GGATCAACTG TGCAGCTGCA GGGAACCCCT TCCCCGTGCG GGGCAGCATA 
GAGCTACGCA AGCCAGACGG CACTGTGCTC CTGTCCACCA AGGCCATTGT GGAGCCAGAG 
AAGACCACAG CTGAGTTCGA GGTGCCCCGC TTGGTTCTTG CGGACAGTGG GTTCTGGGAG 
TGCCGTGTGT CCACATCTGG CGGCCAAGAC AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG 
CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC CTGACCAAGC AGAGCCGCCA GCTTGTGGTC 
TCCCCGCTGG TCTCGTTCTC TGGGGATGGA CCCATCTCCA CTGTCCGCCT GCACTACCGG 
CCCCAGGACA GTACCATGGA CTGGTCGACC ATTGTGGTGG ACCCCAGTGA GAACGTGACG 
TTAATGAACC TGAGGCCAAA GACAGGATAC AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG 
GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC ACCCTCATGA CCACAGACTG TCCTGAGCCT 
TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT GTGGAAGGCA CTGACCGGCT GCGAGTGAGC 
TGGTCCTTGC CCTTGGTGCC CGGGCCACTG GTGGGCGACG GTTTCCTGCT GCGCCTGTGG 
GACGGGACAC GGGGGCAGGA GCGGCGGGAG AACGTCTCAT CCCCCCAGGC CCGCACTGCC 
CTCCTGACGG GACTCACGCC TGGCACCCAC TACCAGCTGG ATGTGCAGCT CTACCACTGC 
ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA CACGTGCTTC TGCCCCCCAG TGGGCCTCCA 
GCCCCCCGAC ACCTCCACGC CCAGGCCCTC TCAGACTCCG AGATCCAGCT GACATGGAAG 
CACCCGGAGG CTCTGCCTGG GCCAATATCC AAGTACGTTG TGGAGGTGCA GGTGGCTGGG 
GGTGCAGGAG ACCCACTGTG GATAGACGTG GACAGGCCTG AGGAGACAAG CACCATCATC 
CGTGGCCTCA ACGCCAGCAC GCGCTACCTC TTCCGCATGC GGGCCAGCAT TCAGGGGCTC 
GGGGACTGGA GCAACACAGT AGAAGAGTCC ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC 
CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG GGCCTGGATC AGCAGCTGAT CCTGGCGGTG 
GTGGGCTCCG TGTCTGCCAC CTGCCTCACC ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC 
ATCCGCAGAA GCTGCCTGCA TCGGAGACGC ACCTTCACCT ACCAGTCAGG CTCGGGCGAG 
GAGACCATCC TGCAGTTCAG CTCAGGGACC TTGACACTTA CCCGGCGGCC AAAACTGCAG 
CCCGAGCCCC TGAGCTACCC AGTGCTAGAG TGGGAGGACA TCACCTTTGA GGACCTCATC 
GGGGAGGGGA ACTTCGGCCA GGTCATCCGG GCCATGATCA AGAAGGACGG GCTGAAGATG 
AACGCAGCCA TCAAAATGCT GAAAGAGTAT GCCTCTGAAA ATGACCATCG TGACTTTGCG 
GGAGAACTGG AAGTTCTGTG CAAATTGGGG CATCACCCCA ACATCATCAA CCTCCTGGGG 
GCCTGTAAGA ACCGAGGTTA CTTGTATATC GCTATTGAAT ATGCCCCCTA CGGGAACCTG 
CTAGATTTTC TGCGGAAAAG CCGGGTCCTA GAGACTGACC CAGCTTTTGC TCGAGAGCAT 
GGGACAGCCT CTACCCTTAG CTCCCGGCAG CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT 
GGCATGCAGT ACCTGAGTGA GAAGCAGTTC ATCCACAGGG ACCTGGCTGC CCGGAATGTG 
CTGGTCGGAG AGAACCTAGC CTCCAAGATT GCAGACTTCG GCCTTTCTCG GGGAGAGGAG 
GTTTATGTGA AGAAGACGAT GGGGCGTCTC CCTGTGCGCT GGATGGCCAT TGAGTCCCTG 
AACTACAGTG TCTATACCAC CAAGAGTGAT GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG 
ATAGTGAGCC TTGGAGGTAC ACCCTACTGT GGCATGACCT GTGCCGAGCT CTATGAAAAG 
CTGCCCCAGG GCTACCGCAT GGAGCAGCCT CGAAACTGTG ACGATGAAGT GTACGAGCTG 
ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT GAGCGACCCC CCTTTGCCCA GATTGCGCTA 
CAGCTAGGCC GCATGCTGGA AGCCAGGAAG GCCTATGTGA ACATGTCGCT GTTTGAGAAC 
TTCACTTACG CGGGCATTGA TGCCACAGCT GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240286.1
CDS	230..3511
Translation	MWAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_001253357.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253357.1

ORF Insert Sequence:

ATGTGGGCCG GGGCGGGGAG GGGCTCGGAC GCCTGGGGCC CGCCCCTGCT GCTGGAGAAG 
GACGACCGTA TCGTGCGCAC CCCGCCCGGG CCACCCCTGC GCCTGGCGCG CAACGGTTCG 
CACCAGGTCA CGCTTCGCGG CTTCTCCAAG CCCTCGGACC TCGTGGGCGT CTTCTCCTGC 
GTGGGCGGTG CTGGGGCGCG GCGCACGCGC GTCATCTACG TGCACAACAG CCCTGGAGCC 
CACCTGCTTC CAGACAAGGT CACACACACT GTGAACAAAG GTGACACCGC TGTACTTTCT 
GCACGTGTGC ACAAGGAGAA GCAGACAGAC GTGATCTGGA AGAGCAACGG ATCCTACTTC 
TACACCCTGG ACTGGCATGA AGCCCAGGAT GGGCGGTTCC TGCTGCAGCT CCCAAATGTG 
CAGCCACCAT CGAGCGGCAT CTACAGTGCC ACTTACCTGG AAGCCAGCCC CCTGGGCAGC 
GCCTTCTTTC GGCTCATCGT GCGGGGTTGT GGGGCTGGGC GCTGGGGGCC AGGCTGTACC 
AAGGAGTGCC CAGGTTGCCT ACATGGAGGT GTCTGCCACG ACCATGACGG CGAATGTGTA 
TGCCCCCCTG GCTTCACTGG CACCCGCTGT GAACAGGCCT GCAGAGAGGG CCGTTTTGGG 
CAGAGCTGCC AGGAGCAGTG CCCAGGCATA TCAGGCTGCC GGGGCCTCAC CTTCTGCCTC 
CCAGACCCCT ATGGCTGCTC TTGTGGATCT GGCTGGAGAG GAAGCCAGTG CCAAGAAGCT 
TGTGCCCCTG GTCATTTTGG GGCTGATTGC CGACTCCAGT GCCAGTGTCA GAATGGTGGC 
ACTTGTGACC GGTTCAGTGG TTGTGTCTGC CCCTCTGGGT GGCATGGAGT GCACTGTGAG 
AAGTCAGACC GGATCCCCCA GATCCTCAAC ATGGCCTCAG AACTGGAGTT CAACTTAGAG 
ACGATGCCCC GGATCAACTG TGCAGCTGCA GGGAACCCCT TCCCCGTGCG GGGCAGCATA 
GAGCTACGCA AGCCAGACGG CACTGTGCTC CTGTCCACCA AGGCCATTGT GGAGCCAGAG 
AAGACCACAG CTGAGTTCGA GGTGCCCCGC TTGGTTCTTG CGGACAGTGG GTTCTGGGAG 
TGCCGTGTGT CCACATCTGG CGGCCAAGAC AGCCGGCGCT TCAAGGTCAA TGTGAAAGTG 
CCCCCCGTGC CCCTGGCTGC ACCTCGGCTC CTGACCAAGC AGAGCCGCCA GCTTGTGGTC 
TCCCCGCTGG TCTCGTTCTC TGGGGATGGA CCCATCTCCA CTGTCCGCCT GCACTACCGG 
CCCCAGGACA GTACCATGGA CTGGTCGACC ATTGTGGTGG ACCCCAGTGA GAACGTGACG 
TTAATGAACC TGAGGCCAAA GACAGGATAC AGTGTTCGTG TGCAGCTGAG CCGGCCAGGG 
GAAGGAGGAG AGGGGGCCTG GGGGCCTCCC ACCCTCATGA CCACAGACTG TCCTGAGCCT 
TTGTTGCAGC CGTGGTTGGA GGGCTGGCAT GTGGAAGGCA CTGACCGGCT GCGAGTGAGC 
TGGTCCTTGC CCTTGGTGCC CGGGCCACTG GTGGGCGACG GTTTCCTGCT GCGCCTGTGG 
GACGGGACAC GGGGGCAGGA GCGGCGGGAG AACGTCTCAT CCCCCCAGGC CCGCACTGCC 
CTCCTGACGG GACTCACGCC TGGCACCCAC TACCAGCTGG ATGTGCAGCT CTACCACTGC 
ACCCTCCTGG GCCCGGCCTC GCCCCCTGCA CACGTGCTTC TGCCCCCCAG TGGGCCTCCA 
GCCCCCCGAC ACCTCCACGC CCAGGCCCTC TCAGACTCCG AGATCCAGCT GACATGGAAG 
CACCCGGAGG CTCTGCCTGG GCCAATATCC AAGTACGTTG TGGAGGTGCA GGTGGCTGGG 
GGTGCAGGAG ACCCACTGTG GATAGACGTG GACAGGCCTG AGGAGACAAG CACCATCATC 
CGTGGCCTCA ACGCCAGCAC GCGCTACCTC TTCCGCATGC GGGCCAGCAT TCAGGGGCTC 
GGGGACTGGA GCAACACAGT AGAAGAGTCC ACCCTGGGCA ACGGGCTGCA GGCTGAGGGC 
CCAGTCCAAG AGAGCCGGGC AGCTGAAGAG GGCCTGGATC AGCAGCTGAT CCTGGCGGTG 
GTGGGCTCCG TGTCTGCCAC CTGCCTCACC ATCCTGGCTG CCCTTTTAAC CCTGGTGTGC 
ATCCGCAGAA GCTGCCTGCA TCGGAGACGC ACCTTCACCT ACCAGTCAGG CTCGGGCGAG 
GAGACCATCC TGCAGTTCAG CTCAGGGACC TTGACACTTA CCCGGCGGCC AAAACTGCAG 
CCCGAGCCCC TGAGCTACCC AGTGCTAGAG TGGGAGGACA TCACCTTTGA GGACCTCATC 
GGGGAGGGGA ACTTCGGCCA GGTCATCCGG GCCATGATCA AGAAGGACGG GCTGAAGATG 
AACGCAGCCA TCAAAATGCT GAAAGAGTAT GCCTCTGAAA ATGACCATCG TGACTTTGCG 
GGAGAACTGG AAGTTCTGTG CAAATTGGGG CATCACCCCA ACATCATCAA CCTCCTGGGG 
GCCTGTAAGA ACCGAGGTTA CTTGTATATC GCTATTGAAT ATGCCCCCTA CGGGAACCTG 
CTAGATTTTC TGCGGAAAAG CCGGGTCCTA GAGACTGACC CAGCTTTTGC TCGAGAGCAT 
GGGACAGCCT CTACCCTTAG CTCCCGGCAG CTGCTGCGTT TCGCCAGTGA TGCGGCCAAT 
GGCATGCAGT ACCTGAGTGA GAAGCAGTTC ATCCACAGGG ACCTGGCTGC CCGGAATGTG 
CTGGTCGGAG AGAACCTAGC CTCCAAGATT GCAGACTTCG GCCTTTCTCG GGGAGAGGAG 
GTTTATGTGA AGAAGACGAT GGGGCGTCTC CCTGTGCGCT GGATGGCCAT TGAGTCCCTG 
AACTACAGTG TCTATACCAC CAAGAGTGAT GTCTGGTCCT TTGGAGTCCT TCTTTGGGAG 
ATAGTGAGCC TTGGAGGTAC ACCCTACTGT GGCATGACCT GTGCCGAGCT CTATGAAAAG 
CTGCCCCAGG GCTACCGCAT GGAGCAGCCT CGAAACTGTG ACGATGAAGT GTACGAGCTG 
ATGCGTCAGT GCTGGCGGGA CCGTCCCTAT GAGCGACCCC CCTTTGCCCA GATTGCGCTA 
CAGCTAGGCC GCATGCTGGA AGCCAGGAAG GCCTATGTGA ACATGTCGCT GTTTGAGAAC 
TTCACTTACG CGGGCATTGA TGCCACAGCT GAGGAGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_005271163.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271220.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

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CloneID	OHu33751
Clone ID Related Accession (Same CDS sequence)	XM_005271163.2 , XM_005271163.1
Accession Version	XM_005271163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005271163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGACCGGAT CCCCCAGATC CTCAACATGG CCTCAGAACT GGAGTTCAAC 
TTAGAGACGA TGCCCCGGAT CAACTGTGCA GCTGCAGGGA ACCCCTTCCC CGTGCGGGGC 
AGCATAGAGC TACGCAAGCC AGACGGCACT GTGCTCCTGT CCACCAAGGC CATTGTGGAG 
CCAGAGAAGA CCACAGCTGA GTTCGAGGTG CCCCGCTTGG TTCTTGCGGA CAGTGGGTTC 
TGGGAGTGCC GTGTGTCCAC ATCTGGCGGC CAAGACAGCC GGCGCTTCAA GGTCAATGTG 
AAAGTGCCCC CCGTGCCCCT GGCTGCACCT CGGCTCCTGA CCAAGCAGAG CCGCCAGCTT 
GTGGTCTCCC CGCTGGTCTC GTTCTCTGGG GATGGACCCA TCTCCACTGT CCGCCTGCAC 
TACCGGCCCC AGGACAGTAC CATGGACTGG TCGACCATTG TGGTGGACCC CAGTGAGAAC 
GTGACGTTAA TGAACCTGAG GCCAAAGACA GGATACAGTG TTCGTGTGCA GCTGAGCCGG 
CCAGGGGAAG GAGGAGAGGG GGCCTGGGGG CCTCCCACCC TCATGACCAC AGACTGTCCT 
GAGCCTTTGT TGCAGCCGTG GTTGGAGGGC TGGCATGTGG AAGGCACTGA CCGGCTGCGA 
GTGAGCTGGT CCTTGCCCTT GGTGCCCGGG CCACTGGTGG GCGACGGTTT CCTGCTGCGC 
CTGTGGGACG GGACACGGGG GCAGGAGCGG CGGGAGAACG TCTCATCCCC CCAGGCCCGC 
ACTGCCCTCC TGACGGGACT CACGCCTGGC ACCCACTACC AGCTGGATGT GCAGCTCTAC 
CACTGCACCC TCCTGGGCCC GGCCTCGCCC CCTGCACACG TGCTTCTGCC CCCCAGTGGG 
CCTCCAGCCC CCCGACACCT CCACGCCCAG GCCCTCTCAG ACTCCGAGAT CCAGCTGACA 
TGGAAGCACC CGGAGGCTCT GCCTGGGCCA ATATCCAAGT ACGTTGTGGA GGTGCAGGTG 
GCTGGGGGTG CAGGAGACCC ACTGTGGATA GACGTGGACA GGCCTGAGGA GACAAGCACC 
ATCATCCGTG GCCTCAACGC CAGCACGCGC TACCTCTTCC GCATGCGGGC CAGCATTCAG 
GGGCTCGGGG ACTGGAGCAA CACAGTAGAA GAGTCCACCC TGGGCAACGG GCTGCAGGCT 
GAGGGCCCAG TCCAAGAGAG CCGGGCAGCT GAAGAGGGCC TGGATCAGCA GCTGATCCTG 
GCGGTGGTGG GCTCCGTGTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAACCCTG 
GTGTGCATCC GCAGAAGCTG CCTGCATCGG AGACGCACCT TCACCTACCA GTCAGGCTCG 
GGCGAGGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CACTTACCCG GCGGCCAAAA 
CTGCAGCCCG AGCCCCTGAG CTACCCAGTG CTAGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAGGTC ATCCGGGCCA TGATCAAGAA GGACGGGCTG 
AAGATGAACG CAGCCATCAA AATGCTGAAA GAGTATGCCT CTGAAAATGA CCATCGTGAC 
TTTGCGGGAG AACTGGAAGT TCTGTGCAAA TTGGGGCATC ACCCCAACAT CATCAACCTC 
CTGGGGGCCT GTAAGAACCG AGGTTACTTG TATATCGCTA TTGAATATGC CCCCTACGGG 
AACCTGCTAG ATTTTCTGCG GAAAAGCCGG GTCCTAGAGA CTGACCCAGC TTTTGCTCGA 
GAGCATGGGA CAGCCTCTAC CCTTAGCTCC CGGCAGCTGC TGCGTTTCGC CAGTGATGCG 
GCCAATGGCA TGCAGTACCT GAGTGAGAAG CAGTTCATCC ACAGGGACCT GGCTGCCCGG 
AATGTGCTGG TCGGAGAGAA CCTAGCCTCC AAGATTGCAG ACTTCGGCCT TTCTCGGGGA 
GAGGAGGTTT ATGTGAAGAA GACGATGGGG CGTCTCCCTG TGCGCTGGAT GGCCATTGAG 
TCCCTGAACT ACAGTGTCTA TACCACCAAG AGTGATGTCT GGTCCTTTGG AGTCCTTCTT 
TGGGAGATAG TGAGCCTTGG AGGTACACCC TACTGTGGCA TGACCTGTGC CGAGCTCTAT 
GAAAAGCTGC CCCAGGGCTA CCGCATGGAG CAGCCTCGAA ACTGTGACGA TGAAGTGTAC 
GAGCTGATGC GTCAGTGCTG GCGGGACCGT CCCTATGAGC GACCCCCCTT TGCCCAGATT 
GCGCTACAGC TAGGCCGCAT GCTGGAAGCC AGGAAGGCCT ATGTGAACAT GTCGCTGTTT 
GAGAACTTCA CTTACGCGGG CATTGATGCC ACAGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271220.1
CDS	119..3406
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_005271163.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271163.2

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGACCGGAT CCCCCAGATC CTCAACATGG CCTCAGAACT GGAGTTCAAC 
TTAGAGACGA TGCCCCGGAT CAACTGTGCA GCTGCAGGGA ACCCCTTCCC CGTGCGGGGC 
AGCATAGAGC TACGCAAGCC AGACGGCACT GTGCTCCTGT CCACCAAGGC CATTGTGGAG 
CCAGAGAAGA CCACAGCTGA GTTCGAGGTG CCCCGCTTGG TTCTTGCGGA CAGTGGGTTC 
TGGGAGTGCC GTGTGTCCAC ATCTGGCGGC CAAGACAGCC GGCGCTTCAA GGTCAATGTG 
AAAGTGCCCC CCGTGCCCCT GGCTGCACCT CGGCTCCTGA CCAAGCAGAG CCGCCAGCTT 
GTGGTCTCCC CGCTGGTCTC GTTCTCTGGG GATGGACCCA TCTCCACTGT CCGCCTGCAC 
TACCGGCCCC AGGACAGTAC CATGGACTGG TCGACCATTG TGGTGGACCC CAGTGAGAAC 
GTGACGTTAA TGAACCTGAG GCCAAAGACA GGATACAGTG TTCGTGTGCA GCTGAGCCGG 
CCAGGGGAAG GAGGAGAGGG GGCCTGGGGG CCTCCCACCC TCATGACCAC AGACTGTCCT 
GAGCCTTTGT TGCAGCCGTG GTTGGAGGGC TGGCATGTGG AAGGCACTGA CCGGCTGCGA 
GTGAGCTGGT CCTTGCCCTT GGTGCCCGGG CCACTGGTGG GCGACGGTTT CCTGCTGCGC 
CTGTGGGACG GGACACGGGG GCAGGAGCGG CGGGAGAACG TCTCATCCCC CCAGGCCCGC 
ACTGCCCTCC TGACGGGACT CACGCCTGGC ACCCACTACC AGCTGGATGT GCAGCTCTAC 
CACTGCACCC TCCTGGGCCC GGCCTCGCCC CCTGCACACG TGCTTCTGCC CCCCAGTGGG 
CCTCCAGCCC CCCGACACCT CCACGCCCAG GCCCTCTCAG ACTCCGAGAT CCAGCTGACA 
TGGAAGCACC CGGAGGCTCT GCCTGGGCCA ATATCCAAGT ACGTTGTGGA GGTGCAGGTG 
GCTGGGGGTG CAGGAGACCC ACTGTGGATA GACGTGGACA GGCCTGAGGA GACAAGCACC 
ATCATCCGTG GCCTCAACGC CAGCACGCGC TACCTCTTCC GCATGCGGGC CAGCATTCAG 
GGGCTCGGGG ACTGGAGCAA CACAGTAGAA GAGTCCACCC TGGGCAACGG GCTGCAGGCT 
GAGGGCCCAG TCCAAGAGAG CCGGGCAGCT GAAGAGGGCC TGGATCAGCA GCTGATCCTG 
GCGGTGGTGG GCTCCGTGTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAACCCTG 
GTGTGCATCC GCAGAAGCTG CCTGCATCGG AGACGCACCT TCACCTACCA GTCAGGCTCG 
GGCGAGGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CACTTACCCG GCGGCCAAAA 
CTGCAGCCCG AGCCCCTGAG CTACCCAGTG CTAGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAGGTC ATCCGGGCCA TGATCAAGAA GGACGGGCTG 
AAGATGAACG CAGCCATCAA AATGCTGAAA GAGTATGCCT CTGAAAATGA CCATCGTGAC 
TTTGCGGGAG AACTGGAAGT TCTGTGCAAA TTGGGGCATC ACCCCAACAT CATCAACCTC 
CTGGGGGCCT GTAAGAACCG AGGTTACTTG TATATCGCTA TTGAATATGC CCCCTACGGG 
AACCTGCTAG ATTTTCTGCG GAAAAGCCGG GTCCTAGAGA CTGACCCAGC TTTTGCTCGA 
GAGCATGGGA CAGCCTCTAC CCTTAGCTCC CGGCAGCTGC TGCGTTTCGC CAGTGATGCG 
GCCAATGGCA TGCAGTACCT GAGTGAGAAG CAGTTCATCC ACAGGGACCT GGCTGCCCGG 
AATGTGCTGG TCGGAGAGAA CCTAGCCTCC AAGATTGCAG ACTTCGGCCT TTCTCGGGGA 
GAGGAGGTTT ATGTGAAGAA GACGATGGGG CGTCTCCCTG TGCGCTGGAT GGCCATTGAG 
TCCCTGAACT ACAGTGTCTA TACCACCAAG AGTGATGTCT GGTCCTTTGG AGTCCTTCTT 
TGGGAGATAG TGAGCCTTGG AGGTACACCC TACTGTGGCA TGACCTGTGC CGAGCTCTAT 
GAAAAGCTGC CCCAGGGCTA CCGCATGGAG CAGCCTCGAA ACTGTGACGA TGAAGTGTAC 
GAGCTGATGC GTCAGTGCTG GCGGGACCGT CCCTATGAGC GACCCCCCTT TGCCCAGATT 
GCGCTACAGC TAGGCCGCAT GCTGGAAGCC AGGAAGGCCT ATGTGAACAT GTCGCTGTTT 
GAGAACTTCA CTTACGCGGG CATTGATGCC ACAGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_005271163.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271220.1 Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

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CloneID	OHu33751
Clone ID Related Accession (Same CDS sequence)	XM_005271163.2 , XM_005271163.1
Accession Version	XM_005271163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGACCGGAT CCCCCAGATC CTCAACATGG CCTCAGAACT GGAGTTCAAC 
TTAGAGACGA TGCCCCGGAT CAACTGTGCA GCTGCAGGGA ACCCCTTCCC CGTGCGGGGC 
AGCATAGAGC TACGCAAGCC AGACGGCACT GTGCTCCTGT CCACCAAGGC CATTGTGGAG 
CCAGAGAAGA CCACAGCTGA GTTCGAGGTG CCCCGCTTGG TTCTTGCGGA CAGTGGGTTC 
TGGGAGTGCC GTGTGTCCAC ATCTGGCGGC CAAGACAGCC GGCGCTTCAA GGTCAATGTG 
AAAGTGCCCC CCGTGCCCCT GGCTGCACCT CGGCTCCTGA CCAAGCAGAG CCGCCAGCTT 
GTGGTCTCCC CGCTGGTCTC GTTCTCTGGG GATGGACCCA TCTCCACTGT CCGCCTGCAC 
TACCGGCCCC AGGACAGTAC CATGGACTGG TCGACCATTG TGGTGGACCC CAGTGAGAAC 
GTGACGTTAA TGAACCTGAG GCCAAAGACA GGATACAGTG TTCGTGTGCA GCTGAGCCGG 
CCAGGGGAAG GAGGAGAGGG GGCCTGGGGG CCTCCCACCC TCATGACCAC AGACTGTCCT 
GAGCCTTTGT TGCAGCCGTG GTTGGAGGGC TGGCATGTGG AAGGCACTGA CCGGCTGCGA 
GTGAGCTGGT CCTTGCCCTT GGTGCCCGGG CCACTGGTGG GCGACGGTTT CCTGCTGCGC 
CTGTGGGACG GGACACGGGG GCAGGAGCGG CGGGAGAACG TCTCATCCCC CCAGGCCCGC 
ACTGCCCTCC TGACGGGACT CACGCCTGGC ACCCACTACC AGCTGGATGT GCAGCTCTAC 
CACTGCACCC TCCTGGGCCC GGCCTCGCCC CCTGCACACG TGCTTCTGCC CCCCAGTGGG 
CCTCCAGCCC CCCGACACCT CCACGCCCAG GCCCTCTCAG ACTCCGAGAT CCAGCTGACA 
TGGAAGCACC CGGAGGCTCT GCCTGGGCCA ATATCCAAGT ACGTTGTGGA GGTGCAGGTG 
GCTGGGGGTG CAGGAGACCC ACTGTGGATA GACGTGGACA GGCCTGAGGA GACAAGCACC 
ATCATCCGTG GCCTCAACGC CAGCACGCGC TACCTCTTCC GCATGCGGGC CAGCATTCAG 
GGGCTCGGGG ACTGGAGCAA CACAGTAGAA GAGTCCACCC TGGGCAACGG GCTGCAGGCT 
GAGGGCCCAG TCCAAGAGAG CCGGGCAGCT GAAGAGGGCC TGGATCAGCA GCTGATCCTG 
GCGGTGGTGG GCTCCGTGTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAACCCTG 
GTGTGCATCC GCAGAAGCTG CCTGCATCGG AGACGCACCT TCACCTACCA GTCAGGCTCG 
GGCGAGGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CACTTACCCG GCGGCCAAAA 
CTGCAGCCCG AGCCCCTGAG CTACCCAGTG CTAGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAGGTC ATCCGGGCCA TGATCAAGAA GGACGGGCTG 
AAGATGAACG CAGCCATCAA AATGCTGAAA GAGTATGCCT CTGAAAATGA CCATCGTGAC 
TTTGCGGGAG AACTGGAAGT TCTGTGCAAA TTGGGGCATC ACCCCAACAT CATCAACCTC 
CTGGGGGCCT GTAAGAACCG AGGTTACTTG TATATCGCTA TTGAATATGC CCCCTACGGG 
AACCTGCTAG ATTTTCTGCG GAAAAGCCGG GTCCTAGAGA CTGACCCAGC TTTTGCTCGA 
GAGCATGGGA CAGCCTCTAC CCTTAGCTCC CGGCAGCTGC TGCGTTTCGC CAGTGATGCG 
GCCAATGGCA TGCAGTACCT GAGTGAGAAG CAGTTCATCC ACAGGGACCT GGCTGCCCGG 
AATGTGCTGG TCGGAGAGAA CCTAGCCTCC AAGATTGCAG ACTTCGGCCT TTCTCGGGGA 
GAGGAGGTTT ATGTGAAGAA GACGATGGGG CGTCTCCCTG TGCGCTGGAT GGCCATTGAG 
TCCCTGAACT ACAGTGTCTA TACCACCAAG AGTGATGTCT GGTCCTTTGG AGTCCTTCTT 
TGGGAGATAG TGAGCCTTGG AGGTACACCC TACTGTGGCA TGACCTGTGC CGAGCTCTAT 
GAAAAGCTGC CCCAGGGCTA CCGCATGGAG CAGCCTCGAA ACTGTGACGA TGAAGTGTAC 
GAGCTGATGC GTCAGTGCTG GCGGGACCGT CCCTATGAGC GACCCCCCTT TGCCCAGATT 
GCGCTACAGC TAGGCCGCAT GCTGGAAGCC AGGAAGGCCT ATGTGAACAT GTCGCTGTTT 
GAGAACTTCA CTTACGCGGG CATTGATGCC ACAGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271220.1
CDS	121..3408
Translation	MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVSATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_005271163.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271163.1

ORF Insert Sequence:

ATGGTCTGGC GGGTGCCCCC TTTCTTGCTC CCCATCCTCT TCTTGGCTTC TCATGTGGGC 
GCGGCGGTGG ACCTGACGCT GCTGGCCAAC CTGCGGCTCA CGGACCCCCA GCGCTTCTTC 
CTGACTTGCG TGTCTGGGGA GGCCGGGGCG GGGAGGGGCT CGGACGCCTG GGGCCCGCCC 
CTGCTGCTGG AGAAGGACGA CCGTATCGTG CGCACCCCGC CCGGGCCACC CCTGCGCCTG 
GCGCGCAACG GTTCGCACCA GGTCACGCTT CGCGGCTTCT CCAAGCCCTC GGACCTCGTG 
GGCGTCTTCT CCTGCGTGGG CGGTGCTGGG GCGCGGCGCA CGCGCGTCAT CTACGTGCAC 
AACAGCCCTG GAGCCCACCT GCTTCCAGAC AAGGTCACAC ACACTGTGAA CAAAGGTGAC 
ACCGCTGTAC TTTCTGCACG TGTGCACAAG GAGAAGCAGA CAGACGTGAT CTGGAAGAGC 
AACGGATCCT ACTTCTACAC CCTGGACTGG CATGAAGCCC AGGATGGGCG GTTCCTGCTG 
CAGCTCCCAA ATGTGCAGCC ACCATCGAGC GGCATCTACA GTGCCACTTA CCTGGAAGCC 
AGCCCCCTGG GCAGCGCCTT CTTTCGGCTC ATCGTGCGGG GTTGTGGGGC TGGGCGCTGG 
GGGCCAGGCT GTACCAAGGA GTGCCCAGGT TGCCTACATG GAGGTGTCTG CCACGACCAT 
GACGGCGAAT GTGTATGCCC CCCTGGCTTC ACTGGCACCC GCTGTGAACA GGCCTGCAGA 
GAGGGCCGTT TTGGGCAGAG CTGCCAGGAG CAGTGCCCAG GCATATCAGG CTGCCGGGGC 
CTCACCTTCT GCCTCCCAGA CCCCTATGGC TGCTCTTGTG GATCTGGCTG GAGAGGAAGC 
CAGTGCCAAG AAGACCGGAT CCCCCAGATC CTCAACATGG CCTCAGAACT GGAGTTCAAC 
TTAGAGACGA TGCCCCGGAT CAACTGTGCA GCTGCAGGGA ACCCCTTCCC CGTGCGGGGC 
AGCATAGAGC TACGCAAGCC AGACGGCACT GTGCTCCTGT CCACCAAGGC CATTGTGGAG 
CCAGAGAAGA CCACAGCTGA GTTCGAGGTG CCCCGCTTGG TTCTTGCGGA CAGTGGGTTC 
TGGGAGTGCC GTGTGTCCAC ATCTGGCGGC CAAGACAGCC GGCGCTTCAA GGTCAATGTG 
AAAGTGCCCC CCGTGCCCCT GGCTGCACCT CGGCTCCTGA CCAAGCAGAG CCGCCAGCTT 
GTGGTCTCCC CGCTGGTCTC GTTCTCTGGG GATGGACCCA TCTCCACTGT CCGCCTGCAC 
TACCGGCCCC AGGACAGTAC CATGGACTGG TCGACCATTG TGGTGGACCC CAGTGAGAAC 
GTGACGTTAA TGAACCTGAG GCCAAAGACA GGATACAGTG TTCGTGTGCA GCTGAGCCGG 
CCAGGGGAAG GAGGAGAGGG GGCCTGGGGG CCTCCCACCC TCATGACCAC AGACTGTCCT 
GAGCCTTTGT TGCAGCCGTG GTTGGAGGGC TGGCATGTGG AAGGCACTGA CCGGCTGCGA 
GTGAGCTGGT CCTTGCCCTT GGTGCCCGGG CCACTGGTGG GCGACGGTTT CCTGCTGCGC 
CTGTGGGACG GGACACGGGG GCAGGAGCGG CGGGAGAACG TCTCATCCCC CCAGGCCCGC 
ACTGCCCTCC TGACGGGACT CACGCCTGGC ACCCACTACC AGCTGGATGT GCAGCTCTAC 
CACTGCACCC TCCTGGGCCC GGCCTCGCCC CCTGCACACG TGCTTCTGCC CCCCAGTGGG 
CCTCCAGCCC CCCGACACCT CCACGCCCAG GCCCTCTCAG ACTCCGAGAT CCAGCTGACA 
TGGAAGCACC CGGAGGCTCT GCCTGGGCCA ATATCCAAGT ACGTTGTGGA GGTGCAGGTG 
GCTGGGGGTG CAGGAGACCC ACTGTGGATA GACGTGGACA GGCCTGAGGA GACAAGCACC 
ATCATCCGTG GCCTCAACGC CAGCACGCGC TACCTCTTCC GCATGCGGGC CAGCATTCAG 
GGGCTCGGGG ACTGGAGCAA CACAGTAGAA GAGTCCACCC TGGGCAACGG GCTGCAGGCT 
GAGGGCCCAG TCCAAGAGAG CCGGGCAGCT GAAGAGGGCC TGGATCAGCA GCTGATCCTG 
GCGGTGGTGG GCTCCGTGTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAACCCTG 
GTGTGCATCC GCAGAAGCTG CCTGCATCGG AGACGCACCT TCACCTACCA GTCAGGCTCG 
GGCGAGGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CACTTACCCG GCGGCCAAAA 
CTGCAGCCCG AGCCCCTGAG CTACCCAGTG CTAGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAGGTC ATCCGGGCCA TGATCAAGAA GGACGGGCTG 
AAGATGAACG CAGCCATCAA AATGCTGAAA GAGTATGCCT CTGAAAATGA CCATCGTGAC 
TTTGCGGGAG AACTGGAAGT TCTGTGCAAA TTGGGGCATC ACCCCAACAT CATCAACCTC 
CTGGGGGCCT GTAAGAACCG AGGTTACTTG TATATCGCTA TTGAATATGC CCCCTACGGG 
AACCTGCTAG ATTTTCTGCG GAAAAGCCGG GTCCTAGAGA CTGACCCAGC TTTTGCTCGA 
GAGCATGGGA CAGCCTCTAC CCTTAGCTCC CGGCAGCTGC TGCGTTTCGC CAGTGATGCG 
GCCAATGGCA TGCAGTACCT GAGTGAGAAG CAGTTCATCC ACAGGGACCT GGCTGCCCGG 
AATGTGCTGG TCGGAGAGAA CCTAGCCTCC AAGATTGCAG ACTTCGGCCT TTCTCGGGGA 
GAGGAGGTTT ATGTGAAGAA GACGATGGGG CGTCTCCCTG TGCGCTGGAT GGCCATTGAG 
TCCCTGAACT ACAGTGTCTA TACCACCAAG AGTGATGTCT GGTCCTTTGG AGTCCTTCTT 
TGGGAGATAG TGAGCCTTGG AGGTACACCC TACTGTGGCA TGACCTGTGC CGAGCTCTAT 
GAAAAGCTGC CCCAGGGCTA CCGCATGGAG CAGCCTCGAA ACTGTGACGA TGAAGTGTAC 
GAGCTGATGC GTCAGTGCTG GCGGGACCGT CCCTATGAGC GACCCCCCTT TGCCCAGATT 
GCGCTACAGC TAGGCCGCAT GCTGGAAGCC AGGAAGGCCT ATGTGAACAT GTCGCTGTTT 
GAGAACTTCA CTTACGCGGG CATTGATGCC ACAGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TIE1
Entrez Gene ID	7075
Full Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
Synonyms	JTK14,TIE
General protein information	Preferred Names tyrosine kinase with immunoglobulin like and EGF like domains 1 Names tyrosine-protein kinase receptor Tie-1 tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.2
Summary	This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	600222

Bos taurus (cattle)	TIE1	NP_776390.1
Rattus norvegicus (Norway rat)	Tie1	NP_445997.1
Gallus gallus (chicken)	TIE1	XP_004936746.1
Danio rerio (zebrafish)	tie1	XP_001334673.5
Pan troglodytes (chimpanzee)	TIE1	XP_001173341.1
Canis lupus familiaris (dog)	TIE1	XP_539652.3
Mus musculus (house mouse)	Tie1	NP_035717.2
Xenopus tropicalis (tropical clawed frog)	tie1	XP_002931650.2
Homo sapiens (human)	TIE1	NP_005415.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TIE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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