BUB1 cDNA ORF clone, Homo sapiens(human)

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22340 NM_004336.5
Latest version!
Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu22228 NM_001278617.1
Latest version!
Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu22242 NM_001278616.1
Latest version!
Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu22340 NM_004336.4 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22340
    Clone ID Related Accession (Same CDS sequence) NM_004336.4 , NM_004336.5
    Accession Version NM_004336.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AF047471.1. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2013 this sequence version replaced NM_004336.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.115773.1, AK291221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004327.1
    CDS113..3370
    Translation

    Target ORF information:

    RefSeq Version NM_004336.4
    Organism Homo sapiens(human)
    Definition Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004336.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22340
    Clone ID Related Accession (Same CDS sequence) NM_004336.4 , NM_004336.5
    Accession Version NM_004336.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AF047471.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004336.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF047471.1, AK291221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302759.11/ ENSP00000302530.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004327.1
    CDS69..3326
    Translation

    Target ORF information:

    RefSeq Version NM_004336.5
    Organism Homo sapiens(human)
    Definition Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004336.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22228
    Clone ID Related Accession (Same CDS sequence) NM_001278617.1
    Accession Version NM_001278617.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Homo sapiens(human)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AK225317.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAGATGA TGAAGATGAT
    TTATCTGCTG GCTTGGCACT GATTGACCTG GGTCAGAGTA TAGATATGAA ACTTTTTCCA
    AAAGGAACTA TATTCACAGC AAAGTGTGAA ACATCTGGTT TTCAGTGTGT TGAGATGCTC
    AGCAACAAAC CATGGAACTA CCAGATCGAT TACTTTGGGG TTGCTGCAAC AGTATATTGC
    ATGCTCTTTG GCACTTACAT GAAAGTGAAA AATGAAGGAG GAGAGTGTAA GCCTGAAGGT
    CTTTTTAGAA GGCTTCCTCA TTTGGATATG TGGAATGAAT TTTTTCATGT TATGTTGAAT
    ATTCCAGATT GTCATCATCT TCCATCTTTG GATTTGTTAA GGCAAAAGCT GAAGAAAGTA
    TTTCAACAAC ACTATACTAA CAAGATTAGG GCCCTACGTA ATAGGCTAAT TGTACTGCTC
    TTAGAATGTA AGCGTTCACG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265546.1
    CDS113..3199
    Translation

    Target ORF information:

    RefSeq Version NM_001278617.1
    Organism Homo sapiens(human)
    Definition Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
    AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAGATGA TGAAGATGAT
    TTATCTGCTG GCTTGGCACT GATTGACCTG GGTCAGAGTA TAGATATGAA ACTTTTTCCA
    AAAGGAACTA TATTCACAGC AAAGTGTGAA ACATCTGGTT TTCAGTGTGT TGAGATGCTC
    AGCAACAAAC CATGGAACTA CCAGATCGAT TACTTTGGGG TTGCTGCAAC AGTATATTGC
    ATGCTCTTTG GCACTTACAT GAAAGTGAAA AATGAAGGAG GAGAGTGTAA GCCTGAAGGT
    CTTTTTAGAA GGCTTCCTCA TTTGGATATG TGGAATGAAT TTTTTCATGT TATGTTGAAT
    ATTCCAGATT GTCATCATCT TCCATCTTTG GATTTGTTAA GGCAAAAGCT GAAGAAAGTA
    TTTCAACAAC ACTATACTAA CAAGATTAGG GCCCTACGTA ATAGGCTAAT TGTACTGCTC
    TTAGAATGTA AGCGTTCACG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22242
    Clone ID Related Accession (Same CDS sequence) NM_001278616.1
    Accession Version NM_001278616.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AK302418.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACACCC CGGAAAATGT CCTTCAATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265545.1
    CDS113..3310
    Translation

    Target ORF information:

    RefSeq Version NM_001278616.1
    Organism Homo sapiens(human)
    Definition Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACACCC CGGAAAATGT CCTTCAATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
    AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG
    AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT
    GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT
    CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT
    GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC
    AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA
    CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT
    AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT
    TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT
    GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG
    AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT
    CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA
    GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG
    CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT
    AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT
    CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT
    GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG
    AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG
    GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA
    GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA
    ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC
    ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT
    CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT
    GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC
    TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA
    CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA
    CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT
    GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC
    ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC
    ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT
    TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA
    AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG
    CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC
    ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA
    GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC
    CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC
    TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT
    CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG
    GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA
    AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA
    CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT
    GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC
    TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA
    ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA
    GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT
    GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT
    ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA
    CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT
    GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA
    AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT
    TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA
    CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT
    AAGCGTTCAC GAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.