BUB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	699
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Synonyms	BUB1A,BUB1L,hBUB1
General protein information	Preferred Names BUB1 mitotic checkpoint serine/threonine kinase Names mitotic checkpoint serine/threonine-protein kinase BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog budding uninhibited by benzimidazoles 1 homolog mitotic spindle checkpoint kinase putative serine/threonine-protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q13
Summary	This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Disorder MIM:	602452
Disorder Html:	Colorectal cancer with chromosomal instability, somatic (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004336.4	NP_004327.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
NM_001278616.1	NP_001265545.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 2
NM_004336.5	NP_004327.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
NM_001278617.1	NP_001265546.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BUB1	XP_001142040.1
Canis lupus familiaris (dog)	BUB1	XP_005630473.1
Bos taurus (cattle)	BUB1	NP_001095481.1
Gallus gallus (chicken)	BUB1	NP_001012888.1
Homo sapiens (human)	BUB1	NP_004327.1
Rattus norvegicus (Norway rat)	Bub1	NP_001099977.1
Danio rerio (zebrafish)	bub1	XP_002665685.2
Xenopus tropicalis (tropical clawed frog)	bub1	NP_001072830.1
	LOC696598	XP_002808098.1
Mus musculus (house mouse)	Bub1	NP_001106650.1

Gene Ontology
Bibliography

Related articles in PubMed

BubR1 Promotes Bub3-Dependent APC/C Inhibition during Spindle Assembly Checkpoint Signaling.
Overlack K, Bange T, Weissmann F, Faesen AC, Maffini S, Primorac I, M?ller F, Peters JM, Musacchio A
Current biology : CB27(19)2915-2927.e7(2017 Oct)

MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells.
Xu B, Xu T, Liu H, Min Q, Wang S, Song Q
Pharmacology100(5-6)269-282(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions.
Title: Expanding the PP2A Interactome by Defining a B56-Specific SLiM.

Chromosomal rearrangement resulting in BUB1 haploinsufficiency identified in a patient with polycystic liver disease. BUB1 may imply germline causes of genetic instability in PLD.
Title: Chromosomal abnormalities in hepatic cysts point to novel polycystic liver disease genes.

Study shows that BUB1beta and PDZ binding kinase are up-regulated in breast cancer tumors and their expression is associated with improved overall survival (OS) in basal-like tumors but worse OS in luminal and untreated patients.
Title: Transcriptomic analyses identify association between mitotic kinases, PDZ-binding kinase and BUB1, and clinical outcome in breast cancer.

Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation.
Title: The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation.

Inhibition of Bub1 kinase impaired chromosome arm resolution but exerted only minor effects on mitotic progression or spindle assembly checkpoint function.
Title: Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524.

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22340	NM_004336.5 Latest version!	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu22228	NM_001278617.1 Latest version!	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu22242	NM_001278616.1 Latest version!	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu22340	NM_004336.4	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( NM_004336.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004327.1 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22340
Clone ID Related Accession (Same CDS sequence)	NM_004336.4 , NM_004336.5
Accession Version	NM_004336.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AF047471.1. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2013 this sequence version replaced NM_004336.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.115773.1, AK291221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004327.1
CDS	113..3370
Translation	MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK

Target ORF information:

RefSeq Version	NM_004336.4
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004336.4

ORF Insert Sequence:

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( NM_004336.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004327.1 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22340
Clone ID Related Accession (Same CDS sequence)	NM_004336.4 , NM_004336.5
Accession Version	NM_004336.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AF047471.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004336.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF047471.1, AK291221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302759.11/ ENSP00000302530.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004327.1
CDS	69..3326
Translation	MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK

Target ORF information:

RefSeq Version	NM_004336.5
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004336.5

ORF Insert Sequence:

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( NM_001278617.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265546.1 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22228
Clone ID Related Accession (Same CDS sequence)	NM_001278617.1
Accession Version	NM_001278617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AK225317.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAGATGA TGAAGATGAT 
TTATCTGCTG GCTTGGCACT GATTGACCTG GGTCAGAGTA TAGATATGAA ACTTTTTCCA 
AAAGGAACTA TATTCACAGC AAAGTGTGAA ACATCTGGTT TTCAGTGTGT TGAGATGCTC 
AGCAACAAAC CATGGAACTA CCAGATCGAT TACTTTGGGG TTGCTGCAAC AGTATATTGC 
ATGCTCTTTG GCACTTACAT GAAAGTGAAA AATGAAGGAG GAGAGTGTAA GCCTGAAGGT 
CTTTTTAGAA GGCTTCCTCA TTTGGATATG TGGAATGAAT TTTTTCATGT TATGTTGAAT 
ATTCCAGATT GTCATCATCT TCCATCTTTG GATTTGTTAA GGCAAAAGCT GAAGAAAGTA 
TTTCAACAAC ACTATACTAA CAAGATTAGG GCCCTACGTA ATAGGCTAAT TGTACTGCTC 
TTAGAATGTA AGCGTTCACG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265546.1
CDS	113..3199
Translation	MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK

Target ORF information:

RefSeq Version	NM_001278617.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278617.1

ORF Insert Sequence:

ATGGACACCC CGGAAAATGT CCTTCAGATG CTTGAAGCCC ACATGCAGAG CTACAAGGGC 
AATGACCCTC TTGGTGAATG GGAAAGATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAGATGA TGAAGATGAT 
TTATCTGCTG GCTTGGCACT GATTGACCTG GGTCAGAGTA TAGATATGAA ACTTTTTCCA 
AAAGGAACTA TATTCACAGC AAAGTGTGAA ACATCTGGTT TTCAGTGTGT TGAGATGCTC 
AGCAACAAAC CATGGAACTA CCAGATCGAT TACTTTGGGG TTGCTGCAAC AGTATATTGC 
ATGCTCTTTG GCACTTACAT GAAAGTGAAA AATGAAGGAG GAGAGTGTAA GCCTGAAGGT 
CTTTTTAGAA GGCTTCCTCA TTTGGATATG TGGAATGAAT TTTTTCATGT TATGTTGAAT 
ATTCCAGATT GTCATCATCT TCCATCTTTG GATTTGTTAA GGCAAAAGCT GAAGAAAGTA 
TTTCAACAAC ACTATACTAA CAAGATTAGG GCCCTACGTA ATAGGCTAAT TGTACTGCTC 
TTAGAATGTA AGCGTTCACG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( NM_001278616.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265545.1 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22242
Clone ID Related Accession (Same CDS sequence)	NM_001278616.1
Accession Version	NM_001278616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC226101.3 and AK302418.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCC CGGAAAATGT CCTTCAATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265545.1
CDS	113..3310
Translation	MDTPENVLQYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK

Target ORF information:

RefSeq Version	NM_001278616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278616.1

ORF Insert Sequence:

ATGGACACCC CGGAAAATGT CCTTCAATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 
CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 
GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 
AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 
GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 
AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 
CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 
AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 
CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 
GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 
AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 
GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 
GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 
ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 
ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 
CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 
GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 
TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 
CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 
CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 
GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 
ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 
ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 
TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 
AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 
CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 
ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 
GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 
CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 
CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 
GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 
AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 
CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 
GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 
ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 
GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 
GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 
ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 
CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 
GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 
AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 
TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 
CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 
AAGCGTTCAC GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BubR1 Promotes Bub3-Dependent APC/C Inhibition during Spindle Assembly Checkpoint Signaling.
Current biology : CB27(19)2915-2927.e7(2017 Oct)
Overlack K,Bange T,Weissmann F,Faesen AC,Maffini S,Primorac I,M?ller F,Peters JM,Musacchio A

MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells.
Pharmacology100(5-6)269-282(2017)
Xu B,Xu T,Liu H,Min Q,Wang S,Song Q

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BUB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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