TCF20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCF20
Entrez Gene ID	6942
Full Name	transcription factor 20
Synonyms	AR1,SPBP,TCF-20
General protein information	Preferred Names transcription factor 20 Names transcription factor 20 SPRE-binding protein nuclear factor SPBP stromelysin-1 PDGF-responsive element-binding protein stromelysin-1 platelet-derived growth factor-responsive element binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.2\|22q13.3
Summary	This gene encodes a transcription factor that recognizes the platelet-derived growth factor-responsive element in the matrix metalloproteinase 3 promoter. The encoded protein is thought to be a transcriptional coactivator, enhancing the activity of transcription factors such as JUN and SP1. Mutations in this gene are associated with autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].
Disorder MIM:	603107

mRNA and Protein(s)

mRNA	Protein	Name
NM_181492.2	NP_852469.1	transcription factor 20 isoform 2
NM_005650.3	NP_005641.1	transcription factor 20 isoform 1
XM_006724313.3	XP_006724376.1	transcription factor 20 isoform X1
XM_005261722.3	XP_005261779.1	transcription factor 20 isoform X1
XM_011530354.2	XP_011528656.1	transcription factor 20 isoform X3
XM_011530353.2	XP_011528655.1	transcription factor 20 isoform X2

Pathways from BioSystems

Homologies

	TCF20	XP_001107318.2
Canis lupus familiaris (dog)	TCF20	XP_857454.2
Rattus norvegicus (Norway rat)	Tcf20	NP_001124046.1
Homo sapiens (human)	TCF20	NP_005641.1
Pan troglodytes (chimpanzee)	TCF20	XP_003317325.1
Mus musculus (house mouse)	Tcf20	NP_001107612.1
Gallus gallus (chicken)	TCF20	XP_004937744.1
Xenopus tropicalis (tropical clawed frog)	tcf20	XP_004913850.1
Bos taurus (cattle)	TCF20	NP_001179503.1

Gene Ontology
Bibliography

Related articles in PubMed

De novo nonsense and frameshift variants of TCF20 in individuals with intellectual disability and postnatal overgrowth.
Sch?fgen J, Cremer K, Becker J, Wieland T, Zink AM, Kim S, Windheuser IC, Krei? M, Aretz S, Strom TM, Wieczorek D, Engels H
European journal of human genetics : EJHG24(12)1739-1745(2016 12)

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder.
Babbs C, Lloyd D, Pagnamenta AT, Twigg SR, Green J, McGowan SJ, Mirza G, Naples R, Sharma VP, Volpi EV, Buckle VJ, Wall SA, Knight SJ, , Parr JR, Wilkie AO
Journal of medical genetics51(11)737-47(2014 Nov)

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

SPBP is a sulforaphane induced transcriptional coactivator of NRF2 regulating expression of the autophagy receptor p62/SQSTM1.
Darvekar SR, Elvenes J, Brenne HB, Johansen T, Sj?ttem E
PloS one9(1)e85262(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nonsense and one frameshift variant of TCF20 were identified the patients with intellectual disability and postnatal overgrowth. Such de novo variants of TCF20 may represent a novel differential diagnosis in the overgrowth syndrome spectrum.
Title: De novo nonsense and frameshift variants of TCF20 in individuals with intellectual disability and postnatal overgrowth.

mutations in TCF20 are associated with Autism spectrum disorders
Title: De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder.

SPBP is a transcriptional coactivator of NRF2 regulating expression of the autophagy receptor p62.
Title: SPBP is a sulforaphane induced transcriptional coactivator of NRF2 regulating expression of the autophagy receptor p62/SQSTM1.

evolutionary conservation of chromatin binding of SPBP and RAI1
Title: A phylogenetic study of SPBP and RAI1: evolutionary conservation of chromatin binding modules.

Study has identified SPBP as a transcriptional coactivator of AR. We also show that Pax6 inhibits SPBP-mediated enhancement of AR activity on the AR target gene probasin promoter, a repression that was partly reversed by increased expression of SPBP.
Title: Pax6 represses androgen receptor-mediated transactivation by inhibiting recruitment of the coactivator SPBP.

The following TCF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01400	NM_181492.2 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu21192	NM_005650.3 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu01400	XM_011530354.2 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu21192	XM_006724313.3 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu21192	XM_005261722.3 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu53674	XM_011530353.2 Latest version!	Homo sapiens transcription factor 20 (TCF20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCF20 ( NM_181492.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_852469.1 Homo sapiens transcription factor 20 (TCF20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01400
Clone ID Related Accession (Same CDS sequence)	NM_181492.2 , XM_011530354.2
Accession Version	NM_181492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX247885.11, U19345.1 and BU732753.1. On Jul 11, 2013 this sequence version replaced NM_181492.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region resulting in a frameshift, compared to variant 1. Isoform 2 has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U19345.1, SRR1163655.215901.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852469.1
CDS	37..5853
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	NM_181492.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181492.2

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( NM_005650.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005641.1 Homo sapiens transcription factor 20 (TCF20), transcript variant 1, mRNA.

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CloneID	OHu21192
Clone ID Related Accession (Same CDS sequence)	NM_005650.3 , XM_006724313.3 , XM_005261722.3
Accession Version	NM_005650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF851355.1, AB006630.1 and BU732753.1. This sequence is a reference standard in the RefSeqGene project. On or before Sep 25, 2015 this sequence version replaced XM_011530352.1, NM_005650.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: KF851355.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005641.1
CDS	138..6020
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	NM_005650.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005650.3

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_011530354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528656.1 Homo sapiens transcription factor 20 (TCF20), transcript variant X4, mRNA.

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CloneID	OHu01400
Clone ID Related Accession (Same CDS sequence)	NM_181492.2 , XM_011530354.2
Accession Version	XM_011530354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528656.1
CDS	322..6138
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKVRLWR

Target ORF information:

RefSeq Version	XM_011530354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530354.2

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGG TGAGACTGTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_006724313.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724376.1 Homo sapiens transcription factor 20 (TCF20), transcript variant X1, mRNA.

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CloneID	OHu21192
Clone ID Related Accession (Same CDS sequence)	NM_005650.3 , XM_006724313.3 , XM_005261722.3
Accession Version	XM_006724313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724376.1
CDS	322..6204
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_006724313.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724313.3

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_005261722.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261779.1 Homo sapiens transcription factor 20 (TCF20), transcript variant X2, mRNA.

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CloneID	OHu21192
Clone ID Related Accession (Same CDS sequence)	NM_005650.3 , XM_006724313.3 , XM_005261722.3
Accession Version	XM_005261722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005261722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261779.1
CDS	1341..7223
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_005261722.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261722.3

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGC CTCCCCTTCC GTGCCCTCTC 
CCCCCCTTGC AGAACAAGAC CGCGAAAGGC AGCCTCAGCA CAGAGCAGTC GGAGCGGGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF20 ( XM_011530353.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528655.1 Homo sapiens transcription factor 20 (TCF20), transcript variant X3, mRNA.

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CloneID	OHu53674
Clone ID Related Accession (Same CDS sequence)	XM_011530353.2
Accession Version	XM_011530353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGA ACAAGACCGC GAAAGGCAGC 
CTCAGCACAG AGCAGTCGGA GCGGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528655.1
CDS	322..6171
Translation	MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHTPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEPEIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKNKTAKGSLSTEQSERG

Target ORF information:

RefSeq Version	XM_011530353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor 20 (TCF20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530353.2

ORF Insert Sequence:

ATGCAGTCCT TTCGGGAGCA AAGCAGTTAC CACGGAAACC AGCAAAGCTA CCCACAGGAG 
GTACACGGCT CATCCCGGCT AGAAGAGTTC AGCCCTCGTC AGGCCCAGAT GTTCCAGAAT 
TTTGGAGGTA CAGGTGGCAG TAGTGGCAGC AGTGGCAGTG GCAGTGGTGG TGGACGACGA 
GGAGCAGCAG CTGCTGCGGC AGCGATGGCT AGCGAGACCT CTGGCCATCA AGGTTACCAG 
GGTTTCAGGA AAGAGGCTGG AGATTTTTAC TACATGGCAG GCAACAAAGA CCCCGTGACT 
ACAGGAACCC CACAGCCTCC TCAGCGAAGG CCTTCTGGGC CTGTGCAGAG CTATGGACCC 
CCCCAGGGGA GCAGCTTTGG CAATCAGTAT GGGAGTGAGG GTCATGTGGG CCAGTTTCAA 
GCACAGCACT CTGGCCTTGG CGGTGTGTCA CATTATCAGC AGGATTACAC TGGGCCTTTC 
TCTCCAGGGA GTGCTCAGTA CCAACAGCAG GCTTCCAGCC AGCAGCAGCA GCAGCAAGTC 
CAGCAGTTGA GACAACAGCT TTACCAGTCC CATCAGCCCC TGCCACAGGC CACTGGCCAA 
CCAGCATCCA GCTCATCCCA TCTACAGCCA ATGCAGCGGC CCTCAACTCT GCCATCCTCT 
GCTGCTGGTT ACCAGTTAAG AGTGGGTCAG TTTGGCCAAC ACTATCAGTC TTCTGCTTCC 
TCCTCCTCCT CCTCCTCCTT CCCTTCACCA CAGCGTTTTA GCCAGTCTGG ACAGAGCTAT 
GATGGCAGTT ACAATGTGAA TGCTGGATCT CAGTATGAAG GACACAATGT GGGTTCTAAT 
GCACAGGCTT ATGGAACACA ATCCAATTAC AGCTATCAGC CTCAATCTAT GAAGAATTTT 
GAACAGGCAA AGATTCCACA AGGGACCCAA CAGGGGCAGC AGCAGCAGCA ACCGCAGCAA 
CAACAACACC CTTCTCAGCA TGTGATGCAG TATACTAACG CTGCCACCAA GCTGCCCCTG 
CAAAGCCAAG TGGGGCAGTA CAACCAGCCT GAGGTTCCTG TGAGGTCCCC CATGCAGTTT 
CACCAGAACT TCAGCCCCAT TTCTAACCCT TCTCCAGCTG CCTCTGTGGT TCAGTCTCCA 
AGCTGTAGTT CTACCCCATC TCCTCTCATG CAGACTGGGG AGAATCTCCA GTGTGGGCAA 
GGCAGTGTGC CTATGGGTTC CAGAAACAGA ATTTTACAGT TAATGCCTCA ACTCAGTCCA 
ACCCCATCAA TGATGCCCAG TCCTAATTCT CATGCTGCAG GCTTCAAAGG GTTTGGACTA 
GAAGGGGTAC CAGAAAAGCG ACTGACAGAT CCTGGGTTGA GTAGTTTGAG TGCTCTGAGT 
ACTCAAGTGG CCAATCTTCC TAACACTGTC CAGCACATGT TACTTTCTGA TGCCCTGACT 
CCTCAGAAGA AGACCTCCAA GAGGCCCTCA TCTTCCAAGA AAGCAGATAG CTGCACAAAT 
TCTGAAGGCT CCTCACAACC TGAAGAACAG CTGAAGTCCC CTATGGCAGA GTCATTAGAT 
GGAGGCTGCT CCAGCAGTTC AGAGGATCAA GGCGAGAGAG TGCGGCAACT AAGTGGCCAG 
AGCACCAGCT CTGACACCAC CTACAAGGGT GGAGCCTCTG AGAAAGCTGG CTCCTCACCG 
GCACAAGGTG CTCAGAATGA ACCCCCCAGA CTCAATGCTA GTCCTGCCGC AAGAGAAGAG 
GCCACCTCAC CAGGCGCTAA GGACATGCCA TTGTCATCCG ACGGGAACCC AAAGGTTAAT 
GAGAAGACTG TTGGGGTGAT TGTCTCCCGG GAAGCCATGA CAGGTCGGGT AGAAAAGCCT 
GGTGGACAAG ATAAAGGCTC CCAAGAGGAT GATCCTGCAG CCACTCAAAG GCCACCTAGC 
AATGGTGGGG CAAAGGAAAC CAGTCATGCA TCACTTCCCC AGCCAGAGCC TCCAGGAGGA 
GGAGGGAGCA AAGGAAACAA GAATGGCGAT AACAACTCCA ACCATAATGG AGAAGGAAAT 
GGCCAGAGTG GCCACTCTGC AGCGGGCCCT GGTTTTACGA GCAGAACTGA GCCTAGCAAA 
TCTCCTGGAA GTCTGCGCTA TAGTTACAAA GATAGTTTCG GGTCAGCCGT GCCACGAAAT 
GTCAGTGGCT TTCCTCAGTA TCCTACAGGG CAAGAAAAGG GAGATTTCAC TGGCCATGGG 
GAACGAAAGG GTAGAAATGA AAAATTCCCA AGCCTCCTGC AGGAAGTGCT TCAGGGTTAC 
CACCACCACC CTGACAGGAG ATATTCTAGG AGTACTCAAG AGCATCAGGG GATGGCTGGT 
AGCCTAGAAG GAACCACAAG GCCCAATGTC TTGGTTAGTC AAACCAATGA ATTAGCTAGC 
AGGGGCCTTC TGAACAAAAG CATTGGGTCT CTATTAGAAA ATCCCCACTG GGGCCCCTGG 
GAAAGGAAAT CAAGCAGCAC AGCTCCTGAA ATGAAACAGA TCAATTTGAC TGACTATCCA 
ATTCCCAGAA AGTTTGAAAT AGAGCCTCAG TCATCAGCAC ATGAGCCTGG GGGTTCCCTC 
TCTGAAAGAA GATCAGTGAT CTGTGATATT TCTCCACTAA GACAGATTGT CAGGGACCCA 
GGGGCTCACT CACTGGGACA CATGAGTGCC GACACCAGAA TTGGGAGGAA TGACCGTCTC 
AATCCAACTT TAAGTCAGTC GGTCATTCTT CCTGGTGGTT TGGTGTCCAT GGAAACCAAG 
CTGAAATCCC AGAGCGGGCA GATAAAAGAG GAAGACTTTG AACAGTCTAA ATCTCAAGCT 
AGTTTCAACA ACAAGAAATC TGGAGACCAC TGCCATCCTC CTAGCATCAA GCATGAGTCT 
TACCGCGGCA ATGCCAGCCC TGGAGCAGCA ACCCATGATT CCCTTTCAGA CTATGGCCCG 
CAAGACAGCA GACCCACGCC AATGCGGCGG GTCCCTGGCA GAGTTGGTGG TCGGGAGGGC 
ATGAGGGGTC GGTCCCCTTC TCAATATCAT GACTTTGCAG AAAAATTGAA AATGTCTCCT 
GGGCGGAGCA GAGGCCCAGG GGGAGACCCT CATCACATGA ATCCACACAT GACCTTTTCA 
GAGAGGGCTA ACCGGAGTTC TTTACACACT CCCTTTTCTC CCAACTCAGA AACCCTGGCC 
TCTGCTTATC ATGCAAATAC TCGGGCTCAT GCTTATGGGG ACCCTAACGC AGGTTTGAAT 
TCTCAGCTGC ATTATAAGAG ACAGATGTAC CAACAGCAAC CAGAGGAGTA TAAAGACTGG 
AGCAGCGGTT CTGCTCAGGG AGTAATTGCT GCAGCACAGC ACAGGCAGGA GGGGCCACGG 
AAGAGTCCAA GGCAGCAGCA GTTTCTTGAC AGAGTACGGA GCCCTCTGAA AAATGACAAA 
GATGGTATGA TGTATGGCCC ACCAGTGGGG ACTTACCATG ACCCCAGTGC CCAGGAGGCT 
GGGCGCTGCC TAATGTCTAG TGATGGTCTG CCTAACAAGG GCATGGAATT AAAGCATGGC 
TCCCAGAAGT TACAAGAATC CTGTTGGGAT CTTTCTCGGC AAACTTCTCC AGCCAAAAGC 
AGCGGTCCTC CAGGAATGTC CAGTCAAAAA AGGTATGGGC CGCCCCATGA GACTGATGGA 
CATGGACTAG CTGAGGCTAC ACAGTCATCC AAACCTGGTA GTGTTATGCT GAGACTTCCA 
GGCCAGGAGG ATCATTCTTC TCAAAACCCC TTAATCATGA GGAGGCGTGT TCGTTCTTTT 
ATCTCTCCCA TTCCCAGTAA GAGACAGTCA CAAGATGTAA AGAACAGTAG CACTGAAGAT 
AAAGGTCGCC TCCTTCACTC ATCAAAAGAA GGCGCTGATA AAGCATTCAA TTCCTATGCC 
CATCTTTCTC ACAGTCAGGA TATCAAGTCT ATCCCTAAGA GAGATTCCTC CAAGGACCTT 
CCAAGTCCAG ATAGTAGAAA CTGCCCTGCT GTTACCCTCA CAAGCCCTGC TAAGACCAAA 
ATACTGCCCC CACGGAAAGG ACGGGGATTG AAATTGGAAG CTATAGTTCA GAAGATTACA 
TCCCCAAATA TTAGGAGGAG CGCATCTTCG AACAGTGCGG AGGCTGGGGG AGACACGGTT 
ACGCTTGATG ATATACTGTC TTTGAAGAGT GGTCCTCCTG AAGGTGGGAG TGTTGCTGTT 
CAGGATGCTG ACATAGAGAA GAGAAAAGGT GAGGTGGCTT CGGACCTAGT CAGTCCAGCA 
AACCAGGAGT TGCACGTAGA GAAACCTCTT CCAAGGTCTT CAGAAGAGTG GCGTGGCAGC 
GTGGATGACA AAGTGAAGAC AGAGACACAT GCAGAAACAG TTACTGCCGG AAAGGAACCC 
CCTGGTGCCA TGACATCCAC AACCTCACAG AAGCCTGGTA GTAACCAAGG GAGACCAGAT 
GGTTCCCTGG GTGGAACAGC ACCTTTAATC TTTCCAGACT CAAAGAATGT ACCTCCAGTG 
GGCATATTGG CCCCTGAGGC AAACCCCAAG GCTGAAGAGA AGGAGAACGA TACAGTGACG 
ATTTCACCGA AGCAAGAGGG TTTCCCTCCA AAGGGATATT TCCCATCAGG AAAGAAGAAG 
GGGAGACCCA TTGGTAGTGT GAATAAGCAA AAGAAACAGC AGCAGCCACC GCCTCCACCC 
CCTCAGCCCC CACAGATACC AGAAGGTTCT GCAGATGGAG AGCCAAAGCC AAAAAAACAG 
AGGCAAAGGA GGGAGAGAAG GAAGCCTGGG GCCCAGCCGA GGAAGCGAAA AACCAAACAA 
GCAGTTCCCA TTGTGGAACC CCAAGAACCT GAGATCAAAC TAAAATATGC CACCCAGCCA 
CTGGATAAAA CTGATGCCAA GAACAAGTCT TTTTACCCTT ACATCCATGT AGTAAATAAG 
TGTGAACTTG GAGCCGTTTG TACAATCATC AATGCTGAGG AAGAAGAACA GACCAAATTA 
GTGAGGGGCA GGAAGGGTCA GAGGTCACTG ACCCCTCCAC CTAGCAGCAC TGAAAGCAAG 
GCGCTCCCGG CCTCGTCCTT TATGCTGCAG GGACCTGTTG TGACAGAGTC TTCGGTTATG 
GGGCACCTGG TTTGCTGTCT GTGTGGCAAG TGGGCCAGTT ACCGGAACAT GGGTGACCTC 
TTTGGACCTT TTTATCCCCA AGATTATGCA GCCACTCTCC CGAAGAATCC ACCTCCTAAG 
AGGGCCACAG AAATGCAGAG CAAAGTTAAG GTACGGCACA AAAGTGCTTC TAATGGCTCC 
AAGACGGACA CTGAGGAGGA GGAAGAGCAG CAGCAGCAGC AGAAGGAGCA GAGAAGCCTG 
GCCGCACACC CCAGGTTTAA GCGGCGCCAC CGCTCGGAAG ACTGTGGTGG AGGCCCTCGG 
TCCCTGTCCA GGGGGCTCCC TTGTAAAAAA GCAGCCACTG AGGGCAGCAG TGAAAAGACT 
GTTTTGGACT CGAAGCCCTC CGTGCCCACC ACTTCAGAAG GTGGCCCTGA GCTGGAGTTA 
CAAATCCCTG AACTACCTCT TGACAGCAAT GAATTTTGGG TCCATGAGGG TTGTATTCTC 
TGGGCCAATG GAATCTACCT GGTTTGTGGC AGGCTCTATG GCCTGCAGGA AGCGCTGGAA 
ATAGCCAGAG AGATGAAATG TTCCCACTGC CAGGAGGCAG GCGCCACCTT GGGCTGCTAC 
AACAAAGGCT GCTCCTTCCG ATACCATTAC CCGTGTGCCA TTGATGCAGA TTGTTTGCTA 
CATGAGGAGA ACTTCTCGGT GAGGTGCCCT AAGCACAAGA ACAAGACCGC GAAAGGCAGC 
CTCAGCACAG AGCAGTCGGA GCGGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De novo nonsense and frameshift variants of TCF20 in individuals with intellectual disability and postnatal overgrowth.
European journal of human genetics : EJHG24(12)1739-1745(2016 12)
Sch?fgen J,Cremer K,Becker J,Wieland T,Zink AM,Kim S,Windheuser IC,Krei? M,Aretz S,Strom TM,Wieczorek D,Engels H

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder.
Journal of medical genetics51(11)737-47(2014 Nov)
Babbs C,Lloyd D,Pagnamenta AT,Twigg SR,Green J,McGowan SJ,Mirza G,Naples R,Sharma VP,Volpi EV,Buckle VJ,Wall SA,Knight SJ,,Parr JR,Wilkie AO

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

SPBP is a sulforaphane induced transcriptional coactivator of NRF2 regulating expression of the autophagy receptor p62/SQSTM1.
PloS one9(1)e85262(2014)
Darvekar SR,Elvenes J,Brenne HB,Johansen T,Sj?ttem E

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TCF20 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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